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UV Vis | UV Vis Spectrophotometer Resources | Thermo Fisher Scientific - SG
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468bd07150dbbf Source: /tf/js/dist/digitaldata.js */ (function () { "use strict"; /** * Invoking a Function as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script> <!-- performance-begin --> <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. Please try again later.", "MINICART_ERROR_2" :"Please enter a quantity in the range of 1 to 999.", "MINICART_ERROR_3" :"Could not add item(s) to cart.", "MINICART_ERROR_4" :"Could not remove item from cart.", "MINICART_ERROR_5" :"Could not create/restore cart.", "MINICART_ERROR_6" :"Unable to retrieve cart details. Please try again later.", "MINICART_MESSAGE_1" :"Adding item(s) to cart...", "MINICART_MESSAGE_2" :"Removing item(s) from cart...", "MINICART_CART_NAME_LABEL_1" :"Recently added to the", "MINICART_NOTE_1" : "Your total price and product details can be viewed in the cart.", MINICART_SUMMARY : "Cart summary", MINICART_PRICE : "Price", MINICART_PRODUCT : "Product", LIST_PRICE : "Price", YOUR_PRICE : "Your price", WEB_PRICE: "Online offer", PRICE_ENDS: "ends", SIGN_IN: "Sign in", //New mini cart CATALOG_NUMBER: "Catalog number:", CONTACT_US: "Contact us", RFQ: "Request quote", LEARN_WHERE_TO_BUY: "Learn where to buy", CURRENCY: "USD", //End new mini cart "CHANGE_CART" :"Change cart", "VIEW_ALL" :"View All", "ITEM" :"item", "ITEMS" :"items", "CLOSE" :"Close", "SUBTOTAL" :"Subtotal", "TOTAL" :"Total", "MANAGE_CART" :"Manage Cart", "FIELD" :"field", "GLOBAL_ERROR_1" :"Please choose one or both of the options for", "GLOBAL_ERROR_2" :"is not a valid e-mail address.", "GLOBAL_ERROR_3" :"Unsupported character(s) were found in field", "GLOBAL_ERROR_4" :"and will be replaced with plain text equivalents (where available). 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class="cmp-separator__horizontal-rule"/> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-d9a54c2393"> <div class="cmp-sectiontitle"> <h2 id="product-literature" class="cmp-sectiontitle__text" data-anchor-nav-title="Product literature">UV-Vis spectrophotometer product literature</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-adb8f05578" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-accordion aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--12"><div id="accordion-812be481dc" class="cmp-accordion" data-cmp-is="accordion"> <div 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href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/uv-vis-spectroscopy-drinking-water-quality-an1217-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: UV-Vis Spectroscopy for Drinking Water Quality: Chlorine and Iron Analysis</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-d7fe3da3e2"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/uv-visible-absorption-measurements-an1218-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Use of UV-Visible Absorption Measurements for Pt-Co Color Analysis and Yellowness Index Calculations</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-312de6f733"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/unlocking-adv-calculation-capabilities-insight-pro-uv-spectroscopy-an1175-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Unlocking Advanced Calculation Capabilities in Insight Pro for UV-Visible Spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-e89c7a093d"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/uvn-peptide-analysis-app-note-mcs-an1180-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Peptide Analysis Use of UV-Visible Second Derivative Spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-712eaa8721"> <a class="cmp-ctaitem__anchor" 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href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/GENESYS-metallic-nanoparticle-app-note-1620150.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: UV-Visible Measurements of Metallic Nanoparticles for Sensing Applications</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-641158259f"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/iqoq-app-note-1529000.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Instrument Qualification: A Guide to IQ/OQ Procedures</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-772da3b76c"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/band-gap-analysis-uv-visible-spectroscopy-an54685-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Band Gap Analysis through UV-Visible Spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-699d6a4281"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/analyzing-curved-surfaces-uv-visible-techniques-an54662-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Analyzing Curved Surfaces with UV-Visible Techniques: Measuring Blue-Light Reductions Lenses</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-a05ca1689a"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/analysis-chlorophyll-food%20products-uv-visible-absorption-an54628-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Analysis of chlorophyll content in food products through UV-Visible absorption measurements</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-4c9bb01172"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets%2FCAD%2FApplication-Notes%2FSolid%20State%20Analysis%20Evo%20App%20Note-AN54650-EN.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Solid-State Materials: Analysis Through UV-Visible Spectroscopic Techniques</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-2a3877e7f0"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/monitoring-enzyme-kinetics-uv-visible-absorption-spectroscopy-an56399.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Monitoring enzyme kinetics using UV-Visible absorption spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-13eda573c2"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/protein-aggregation-uv-visible-absorption-spectroscopy-en-an53585.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Protein aggregation identified through UV-Visible absorption spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-ce40e98b4c"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/analysis-dna-melting-uv-visible-an56369-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Analysis of DNA melting through UV-Visible absorption spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-78b2950995"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/anti-factor-xa-and-IIa-assays-for-unfractioned-heparin-activity-an53623-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Anti-Factor Xa and IIa Assays for Unfractioned Heparin Activity</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-3e0129a5f1"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/AN53072-determination-total-protein-concentration-bca-assay.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Determination of total protein concentration</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-6447fd5815"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/AN53158-spectral-transmission-testing-pharmaceutical-containers.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Spectral transmission testing of pharmaceutical containers: Using an Evolution Spectrophotometer with an ISA-220 Integrating Sphere Accessory for testing according to USP <671> and USP <661.2</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-49fea98120"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/AN53349-spectrophotometric-analysis-ibuprofen.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Spectrophotometric analysis of ibuprofen according to USP and EP monographs</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-66edc1a11e"> <a class="cmp-ctaitem__anchor" href="http://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/color-analysis-pharmaceutical-an56364-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Color analysis for pharmaceutical products using UV-Visible absorption techniques</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-a9b7352cd8"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/vitamin-a-%20quantification-evo-app-note-AN54651.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Analysis of Vitamin A Within Cod Liver Oil: Use for Derivatives in UV-Visible Absorption Techniques</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-b3cf8fc4f1"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/AN54663-angle-dependent-reflection-analysis-evo-app-note-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Angle-dependent reflection analysis of smart phone privacy screens</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-36fcd575c0"> <a class="cmp-ctaitem__anchor" href="http://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/genesys-smartqc-appnote-fl54698-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Application note: Setting pass/fail criteria with GENESYS Smart QC: Sports drink quality testing</span> </a> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-2073612353" class="cmp-text"> <p><span class="h3">Brochures</span></p> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-e2ddad7b0b"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/brochures/BR53086-beercraft-software-brochure.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Brochure: BeerCraft Software</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-5ad750c021"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/brochures/BR53458-evolution-family.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Brochure: Evolution UV-Vis Spectrophotometers </span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-fd3c4b630a"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/brochures/BR53062-uv-vis-fiber-optic-probes.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Brochure: Fiber optic probes</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-fa2a227b71"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/brochures/BR52989-genesys-family-brochure.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Brochure: GENESYS Spectrophotometers</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-d48558e0eb"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/brochures/genesys-uv-vis-spectrophotometer-accessories-brochure-BR52302.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Brochure: GENESYS Spectrophotometer Accessories</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-151080b981"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/brochures/thermo-scientific-insight-pro-software-evolution-uv-vis-spectrophotometers-BR53467.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Brochure: Insight Pro for Evolution</span> </a> </div> </div> <div class="cmp-p-ctaitem 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class="cmp-ctaitem" id="ctaitem-90f6989ccd"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Reference-Materials/CS53348-omega-yeast-testimonial-genesys-spectrophotometer.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Case study: Craft brewing case study - Omega Yeast</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-6bd051efe0"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Reference-Materials/CS53172-pFriem-case-study-beer-quality.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Case study: How pFriem Family Brewers use UV-Vis for Quality Control</span> </a> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-585582267" class="cmp-text"> <p><span class="font-size-22">Compendiums</span></p> </div> </div> <div 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m--btn-link"><div class="cmp-ctaitem" id="ctaitem-420a57743a"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Flyers/FL53176-genesys-beer-analyzers-leasing-flyer.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Flyer: BeerCraft Financing Programs for Craft Brewery Operations </span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-4eececb892"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Flyers/vis-uvvis-spectroscopy-education-solutions-FL52991.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Flyer: Vis and UV-Vis Spectroscopy Solutions for Education</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-e4970b6a96"> <a class="cmp-ctaitem__anchor" 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m--btn-link"><div class="cmp-ctaitem" id="ctaitem-991d8d0326"> <a class="cmp-ctaitem__anchor" href="/sg/en/home/global/forms/industrial/uv-vis-lesson-plan-compendium.html"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Lesson plan: Teaching tomorrow’s scientists today: Lesson plans and resources for UV-Visible spectroscopic techniques</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-6be49780df"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/CAD/Flyers/genesys-od600-measurements-lesson-plan-FL64716.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Lesson plan: Bacterial Growth Curves: Analysis through OD600 measurements</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-f8f6770330"> <a class="cmp-ctaitem__anchor" 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href="https://assets.thermofisher.com/TFS-Assets/MSD/Scientific-Resources/FL53099-food-dyes-beers-law-qc-lesson-plan.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Lesson Plan: Food Dyes and Beer's Law</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-bd1835e26a"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Flyers/FL53103-kinetics-blue-dye-hypochlorite-bleach.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Lesson Plan: Kinetics of Blue Dye with Hypochlorite Bleach</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-6cf0884305"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Flyers/FL53104-spectophotometric-determination-trace-iron-solution.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Lesson Plan: Spectrophotometric Determination of Trace Iron in Solution</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-71a420aa76"> <a class="cmp-ctaitem__anchor" href="http://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/physical-chemistry-kinetics%20lp-fl54692-en.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Lesson Plan: Complex reaction monitoring with UV-Visible absorption spectroscopy: KMnO4 + sugar</span> </a> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-1525324947" class="cmp-text"> <p><span class="h3">Smart notes</span></p> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-e5b5d687ca"> <a class="cmp-ctaitem__anchor" 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href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/SN53076-analyze-beer-color-uv-vis.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Smart Note: How to Analyze Beer Color</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-f53546599f"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/SN53085-analyze-beer-vdk-uv-vis.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Smart Note: How to Analyze Vicinal Diketones (VDK) in Beer</span> </a> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-299470394" class="cmp-text"> <p><span class="h3">Specification sheets</span></p> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-b9b047db8e"> <a class="cmp-ctaitem__anchor" 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href="/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FMSD%2FSpecification-Sheets%2FPS53460-evolution-one-one-plus-spectrophotometer-guaranteed-specifications.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Specification Sheet: Evolution One/One Plus - Guaranteed Specifications</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-9c3fcd17c9"> <a class="cmp-ctaitem__anchor" href="/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FMSD%2FSpecification-Sheets%2FPS53459-evolution-one-one-plus-spectrophotometer-typical-specifications.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Specification Sheet: Evolution One/One Plus - Typical Specifications</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-bf60efbe6a"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Specification-Sheets/PS53461-evolution-pro-spectrophotometer.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Specification Sheet: Evolution Pro Spectrophotometer</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-4b2b14608b"> <a class="cmp-ctaitem__anchor" href="http://assets.thermofisher.com/TFS-Assets/MSD/Specification-Sheets/PS53313-integrating-sphere-accessory.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Specification Sheet: Integrating Sphere Accessory for Evolution One Plus</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-ddc092d42e"> <a class="cmp-ctaitem__anchor" 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</div> <div class="cmp-p-text mt-l1"> <div id="text-1486178063" class="cmp-text"> <p><span class="h3">Technical notes</span></p> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-b344edd5ef"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Technical-Notes/TN52236-differences-bacterial-optical-density-uv-vis.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Technical Note: Differences in Bacterial Optical Density Measurements between UV-Visible Spectrophotometers</span> </a> </div> </div> <div class="cmp-p-text mt-l1"> <div id="text-711849919" class="cmp-text"> <p><span class="h3">User guides</span></p> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-1897735202"> <a class="cmp-ctaitem__anchor" 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class="cmp-ctaitem__text">Security Suite Software for Evolution series and NanoDrop One/OneC Spectrophotometers frequently asked questions (FAQs)</span> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-6df0040640" class="cmp-container"> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-5e45c3dca0"> <div class="cmp-sectiontitle"> <h2 id="webinars" class="cmp-sectiontitle__text" data-anchor-nav-title="Webinars">UV-Vis spectrophotometer webinars</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-a9a6267c9f" class="cmp-container"> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-6bb5cdc811"> <a class="cmp-ctaitem__anchor" href="/sg/en/home/global/forms/industrial/analysis-of-semiconductors-through-uv-visible-techniques.html" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: Analysis of Semiconductors through UV-Visible Techniques</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-55e1a7685a"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/1964492303001/default_default/index.html?videoId=6337943323112" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: UV-Visible Derivative Spectroscopy: Theory and Applications</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-0c9d9e5e5e"> <a class="cmp-ctaitem__anchor" 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<span class="cmp-ctaitem__text">Webinar series: Alternative protein and plant-based meat</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-451eabdf7b"> <a class="cmp-ctaitem__anchor" href="/sg/en/home/global/forms/industrial/dna-melting-temperature-webinar.html"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: DNA Melting Temperature Analysis using UV-Vis Spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-30ccf1f66a"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/665001591001/blevZSYeT_default/index.html?videoId=6296559930001" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: Identification of protein aggregation through UV-Vis Spectroscopy </span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-5594062d4a"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/665001591001/blevZSYeT_default/index.html?videoId=6274435849001" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: Improving quality of bacterial culture and oligonucleotides with UV/Vis spectroscopy to accelerate mRNA vaccine production</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-55a13ebdb9"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/665001591001/blevZSYeT_default/index.html?videoId=5195247486001" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: Improving reliability of UV-Visible spectroscopy data for QA/QC</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-03ee3594ca"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/665001591001/blevZSYeT_default/index.html?videoId=6274435849001" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: mRNA vaccine development and manufacturing workflows enabled by UV-Vis spectroscopy</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-b5d7d1dc44"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/665001591001/I9XluuSon_default/index.html?videoId=6274437796001" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: Revealing the next advancement for Evolution UV-Vis Spectrophotometers - Evolution OnePlusPro</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-d967da4a60"> <a class="cmp-ctaitem__anchor" href="https://players.brightcove.net/665001591001/blevZSYeT_default/index.html?videoId=5192785313001" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: Teaching spectroscopy in the high school chemistry classroom</span> </a> </div> </div> <div class="cmp-p-ctaitem m--btn-link"><div class="cmp-ctaitem" id="ctaitem-45a97b7e52"> <a class="cmp-ctaitem__anchor" href="/sg/en/home/global/forms/industrial/uv-vis-analysis-of-food-samples..html" target="_blank"> <span class="cmp-ctaitem__icon icon-24-playButton-mono"></span> <span class="cmp-ctaitem__text">Webinar: UV-Vis analysis of food samples</span> </a> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-7c974e316f" class="cmp-container"> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-1124d907b3"> <div class="cmp-sectiontitle"> <h2 id="spectroscopy-principles" class="cmp-sectiontitle__text" data-anchor-nav-title="Principles of spectroscopy">UV-Vis spectrophotometer principles</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-57f6eac191" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-image aem-GridColumn aem-GridColumn--default--12"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/visible-light-spectrum-1000x350.jpg" data-asset="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/visible-light-spectrum-1000x350.jpg" data-title="Visible light spectrum" id="image-480143284" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/visible-light-spectrum-1000x350.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Visible light spectrum" title="Visible light spectrum"/> </noscript> <meta itemprop="caption" content="Visible light spectrum"/> </div> <!-- Start Modal--> </div> <div class="cmp-p-image aem-GridColumn aem-GridColumn--default--12"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/spectrophotometers-diagram-1000x350.jpg" data-asset="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/spectrophotometers-diagram-1000x350.jpg" data-title="Spectrophotometry diagram" id="image-1839100103" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/spectrophotometers-diagram-1000x350.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Spectrophotometry diagram" title="Spectrophotometry diagram"/> </noscript> <meta itemprop="caption" content="Spectrophotometry diagram"/> </div> <!-- Start Modal--> </div> <div class="cmp-p-text mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="text-59005075" class="cmp-text"> <p>In the most basic terms, spectrophotometers enable photometric comparisons of relative light intensities across the ultraviolet and visible spectrums. Directing a controlled, constant intensity light source (halogen, deuterium, xenon) across the spectrum or at a specific wavelength through a sample easily can confirm known or calculate unknown characteristics of the sample. The incident light (I<sub>0</sub>) can be redirected backward as <em>reflection</em>, suffer an energy loss as <em>absorption</em>, and pass through transparent or translucent samples as <em>transmission</em>. Samples that are in liquids need to be placed into a test tube or cuvette composed of plastic, glass, or quartz that is compatible with the sample and the measurement wavelength, and placed into a holder in the sample compartment before a measurement can be made. </p> <p> </p> <p>Spectrometers can have different optical setups with a single, double (or split), or dual beam setup. The simplest and most economical units rely on a single beam of light that travels from the wavelength selector (monochromator or filter system) to the detector, but requires separate measurements of the baseline solvent blank to compare with the measurement of the sample. A double beam unit splits the light beam into a reference beam and a sample beam, enabling measurement of a blank and a sample in the sample compartment under the same instrument conditions with a single detector that alternates data collection between the reference and the sample. A dual beam setup uses two beams and two detectors in parallel to measure the sample and reference simultaneously. </p> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-70ed670b44" class="cmp-container"> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-a1399775c3"> <div class="cmp-sectiontitle"> <h2 id="absorbance" class="cmp-sectiontitle__text" data-anchor-nav-title="Absorbance">UV-Vis spectrophotometer absorption</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-1b86f687fa" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 m--match-height aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-dd089cf346" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1788663640" class="cmp-text"> <p>When samples are irradiated with light, they selectively absorb incident light at specific wavelengths. The wavelength with the highest absorbance (λ<sub>max</sub>) is typically used as the analytical wavelength and expressed in nanometers (nm). Absorbance measurements are simple to take and are used to generate spectrum curves. </p> <p> </p> <p>Absorption can provide direct and indirect options for calculating concentration. An example of direct measurement for a protein is at A280, which then allows the calculation of the protein concentration (c) based directly on the sample absorbance (A) at 280 nm and the protein-specific extinction coefficient (ε) using the Beer-Lambert equation c = A / (ε × L), where L is the pathlength. An indirect measurement, such as a colorimetric assay, relies on the generation of a standard curve and a reaction that produces a color change proportional to amount of protein in the sample. </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 mt-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-c2fc6fd3f9" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/spectra-protein-standards-and-resulting-calibration-curve-720x480.jpg" data-asset="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/spectra-protein-standards-and-resulting-calibration-curve-720x480.jpg" data-title="Spectra of protein standards and resulting calibration curve" id="image-1577561959" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/spectra-protein-standards-and-resulting-calibration-curve-720x480.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Spectra of protein standards and resulting calibration curve" title="Spectra of protein standards and resulting calibration curve"/> </noscript> <meta itemprop="caption" content="Spectra of protein standards and resulting calibration curve"/> </div> <!-- Start Modal--> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-bc99963f92" class="cmp-container"> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-1472abf9d2"> <div class="cmp-sectiontitle"> <h2 id="spectral-bandwidth" class="cmp-sectiontitle__text" data-anchor-nav-title="Spectral bandwidth">UV-Vis spectrophotometer spectral bandwidth</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--12 aem-GridColumn--offset--default--0"> <div id="container-09428249a6" class="cmp-container"> <div class="aem-Grid aem-Grid--default--12 "> <div class="cmp-p-text mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="text-1470042365" class="cmp-text"> <p>The slit size coming out of the monochromator governs the spectral bandwidth (or bandpass) of the instrument and affects the ability to discriminate between two neighboring peaks (spectral resolution), the intensity of light that reaches the detector, the measurement time, and the signal-to-noise ratio. A narrower bandwidth spectrophotometer can increase resolution and the signal-to-noise ratio at the expense of measurement duration; a more economical instrument with a wider bandwidth can decrease time-to-results at the expense of decreased resolution and increased background noise. To meet the performance and bandwidth requirements for all global pharmacopoeias including United States Pharmacopoeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP), you can choose the Thermo Scientific <a href="/sg/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/uv-vis-spectrophotometry/instruments/evolution-uv-vis-spectrophotometer.html#features" data-uw-rm-brl="false">Evolution Pro instrument</a>, or choose the <a href="/sg/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/uv-vis-spectrophotometry/instruments/evolution-uv-vis-spectrophotometer.html#features" data-uw-rm-brl="false">Evolution One series</a> models to meet all requirements for USP and EP.</p> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-bad7237a35" class="cmp-container"> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-1fd48dc430"> <div class="cmp-sectiontitle"> <h2 id="transmittance" class="cmp-sectiontitle__text" data-anchor-nav-title="Transmittance">Percentage of sample transmission</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container aem-GridColumn aem-GridColumn--default--12"> <div id="container-583ce715e8" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 aem-Grid--phone--12 "> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-26b16f1d47" class="cmp-container"> <div class="cmp-p-text mt-l1"> <div id="text-1753854808" class="cmp-text"> <p>Transmittance measures the amount of light that passes through a sample and can provide a quantitative information based on known reflective and transmissive properties of a material. Transmittance values are typically reported as a percentage, comparing the ratio of the light reaching the detector to the incident light entering the sample. A sample that is perfectly transparent transmits all the light (100% T); a completely opaque sample transmits none of the light (0% T). This measurement can be used to calculate the concentration of chemicals in solution, test the clarity of water and thin films, assess the grade of products like maple syrup, and more. </p> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn--default--none aem-GridColumn--phone--none aem-GridColumn--phone--12 aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--phone--0 aem-GridColumn--offset--default--0"> <div id="container-0d4408e71b" class="cmp-container"> <div class="cmp-p-image"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/beer-lambert-law-illustration-720x480.jpg" data-asset="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/beer-lambert-law-illustration-720x480.jpg" data-title="Beer-Lambert Law illustration" id="image-154272754" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/beer-lambert-law-illustration-720x480.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="Beer-Lambert Law illustration" title="Beer-Lambert Law illustration"/> </noscript> <meta itemprop="caption" content="Beer-Lambert Law illustration"/> </div> <!-- Start Modal--> </div> <div class="cmp-p-text mt-l1"> <div id="text-1796459512" class="cmp-text"> <p><span class="muted"><span class="font-size-14">To calculate transmittance (T), the amount of light exiting the sample (I) is divided by the amount of incident light entering the sample (I<sub>0</sub>): T= I/I<sub>0</sub>. Typically, these measurements are reported as percentage transmittance by multiplying by 100: %T= 100(I/I<sub>0</sub>). There is a logarithmic relationship between absorbance and transmittance, where A = - log<sub>10</sub>T: when absorbance equals 0, transmittance is 100% T and when absorbance equals 1 transmittance is 10%.</span></span></p> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-f5bbb933ac" class="cmp-container"> <div class="cmp-separator"> <hr class="cmp-separator__horizontal-rule"/> </div> </div> </div> </div> </div> </div> <div class="cmp-p-sectioncontainer m--indent-l1"><div class="cmp-sectioncontainer" id="sectioncontainer-b19543d327"> <div class="cmp-sectiontitle"> <h2 id="reflectance" class="cmp-sectiontitle__text" data-anchor-nav-title="Reflectance">Percentage of reflectance measurements</h2> </div> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-db96ae5441" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-text mt-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="text-1658720050" class="cmp-text"> <p>Samples in solid or solution form can reflect the incident light used for spectrophotometric analysis. When a surface is very smooth, it can produce a mirror-like, specular reflection, where the incident ray of light reflects as a single ray of light at a reflection angle that equals the incident angle. Rough surfaces and those that scatter light inside the sample instead produce a diffuse reflection where even a single angle of incidence can result in a reflection that dissipates through a broad distribution of angles. Measuring the transmission of light of these materials using a traditional sample holder will result in only a fraction of light reaching the detector, leading to reduced spectral quality and information loss. However, using an <a href="/order/catalog/product/222-269400?SID=srch-hj-222-269400" data-uw-rm-brl="false">integrating sphere accessory</a> enables all light passing through the sample to be collected for total transmission measurements by positioning the sample at the entrance point or for total or diffuse reflectance measurements by positioning the sample at the exit port.</p> </div> </div> <div class="cmp-p-image aem-GridColumn aem-GridColumn--default--12"> <div data-cmp-is="image" data-cmp-src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/integrating-sphere-1000x350.jpg" data-asset="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/integrating-sphere-1000x350.jpg" data-title="With an integrating sphere accessory, you can position the sample at the entrance point to collect total transmission data or at the exit point for total or diffuse reflectance measurements." id="image-109343224" class="cmp-image " itemscope itemtype="http://schema.org/ImageObject"> <noscript data-cmp-hook-image="noscript"> <img src="/content/dam/tfsite/images/storefront/spectroscopy/uv-vis/integrating-sphere-1000x350.jpg" class="cmp-image__image" loading="lazy" itemprop="contentUrl" data-cmp-hook-image="image" alt="With an integrating sphere accessory, you can position the sample at the entrance point to collect total transmission data or at the exit point for total or diffuse reflectance measurements." title="With an integrating sphere accessory, you can position the sample at the entrance point to collect total transmission data or at the exit point for total or diffuse reflectance measurements."/> </noscript> <meta itemprop="caption" content="With an integrating sphere accessory, you can position the sample at the entrance point to collect total transmission data or at the exit point for total or diffuse reflectance measurements."/> </div> <!-- Start Modal--> </div> </div> </div> </div> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-d298eea6d9" class="cmp-container"> <div class="cmp-p-ctaitem"><div class="cmp-ctaitem" id="ctaitem-39917bcb6c"> <a class="cmp-ctaitem__anchor" href="https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/AN53158-spectral-transmission-testing-pharmaceutical-containers.pdf" target="_blank"> <span class="cmp-ctaitem__icon icon-24-document-mono"></span> <span class="cmp-ctaitem__text">Read our application note: Spectral transmission testing of pharmaceutical containers</span> </a> </div> </div> </div> </div> </div> </div> </div> <div class="cmp-p-experiencefragment"><div id="experiencefragment-dedf7a95fa" class="cmp-experiencefragment cmp-experiencefragment--fruo-legal-statement"> <div id="container-eb94143b1a" class="cmp-container"> <div class="aem-Grid aem-Grid--12 aem-Grid--default--12 "> <div class="cmp-p-container p-l1 aem-GridColumn aem-GridColumn--default--12"> <div id="container-6984ebfee2" class="cmp-container"> <div class="cmp-p-text"> <div id="text-93296864" class="cmp-text"> <p>For Research Use Only. 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