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class="highwire-journal-article-marker-start"></span><h1 id="article-title-1" itemprop="headline">EDTA</h1> <p id="p-1"><a href="/lookup/doi/10.1101/pdb.caut2769" class="caution"></a>EDTA (ethylenediamenetetraacetic acid) </p> <p id="p-2"><a href="/lookup/doi/10.1101/pdb.caut2249" class="caution"></a>NaOH </p> <p id="p-3">To prepare EDTA at 0.5 M (pH 8.0): Add 186.1 g of disodium EDTA•2H<sub>2</sub>O to 800 mL of H<sub>2</sub>O. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (~20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving. The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to ~8.0 by the addition of NaOH. </p><span class="highwire-journal-article-marker-end"></span></div> <div class="social-bookmarking"> <ul class="social-bookmark-links"> <li class="social-bookmarking-item social-bookmarking-item-citeulike"><a href="/external-ref?tag_url=http://cshprotocols.cshlp.org/cgi/content/long/2006/1/pdb.rec8030&title=EDTA+--+%202006%20%281%29%3A%20pdb.rec8030+--+0-87969-577-3&doi=10.1101/pdb.rec8030&link_type=CITEULIKE"><img src="/shared/img/common/social-bookmarking/citeulike.gif" alt="Add to CiteULike" title="CiteULike" /></a><span class="soc-bm-link-text">CiteULike</span></li> <li class="social-bookmarking-item social-bookmarking-item-delicious"><a href="/external-ref?tag_url=http://cshprotocols.cshlp.org/cgi/content/long/2006/1/pdb.rec8030&title=EDTA+--+%202006%20%281%29%3A%20pdb.rec8030+--+0-87969-577-3&doi=10.1101/pdb.rec8030&link_type=DEL_ICIO_US"><img 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