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Search results for: Dunaliella sp.
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style="font-size:1.6rem;">Search results for: Dunaliella sp.</h1> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> The Effect of Temperature and Salinity on the Growth and Carotenogenesis of Three Dunaliella Species (Dunaliella sp. Lake Isolate, D. salina CCAP 19/18, and D. bardawil LB 2538) Cultivated under Laboratory Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Imen%20Hamed">Imen Hamed</a>, <a href="https://publications.waset.org/search?q=Burcu%20Ak"> Burcu Ak</a>, <a href="https://publications.waset.org/search?q=Oya%20I%C5%9F%C4%B1k"> Oya I艧谋k</a>, <a href="https://publications.waset.org/search?q=Leyla%20Uslu"> Leyla Uslu</a>, <a href="https://publications.waset.org/search?q=Kubilay%20Kaz%C4%B1m%20Vursavu%C5%9F"> Kubilay Kaz谋m Vursavu艧</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>In this study, 3 species of <em>Dunaliella</em> (<em>Dunaliella</em> sp. Salt Lake isoalte (Tuz <em>Gölü),</em> <em>Dunaliella salina</em> CCAP19/18, and <em>Dunaliella bardawil</em> LB 2538) and their optical density, dry matter, chlorophyll <em>a, </em>total carotenoids, and β-carotene production were investigated in a batch system. The aim of this research was to compare carotenoids, and β-carotene production were investigated in a batch those 3 species. Therefore 2 stress factors were used: 2 different temperatures (20°C and 30°C) and 2 different salinities (30‰, and 60‰) were tested over a 17-day study. The highest growth and chlorophyll <em>a</em> was reported for <em>Dunaliella </em>sp. under 20°C/30‰ and 20°C/60‰ conditions respectively followed by <em>D. bardawil</em> and <em>D. salina</em>. Significant differences were noticed (p<0.05) for the other 3 species. The growth decreased as temperature and salinity increased since the lowest growth was noticed for the 30°C/60‰ group. The chlorophyll <em>a</em> content decreased also as temperature increased however when the NaCl concentration increased an augmentation of the content was noticed . In the 17<sup>th</sup> day of experiment the highest carotenoids concentration was reported for <em>D. bardawil </em>20°C/30‰ (65,639±0,400 μg.mL<sup>−</sup><sup>1</sup>) and the most important β carotene concentration was for <em>D</em>. <em>salina</em> 20°C/60‰ (8,98E-07±0,013 mol/L).</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Dunaliella%20sp." title="Dunaliella sp.">Dunaliella sp.</a>, <a href="https://publications.waset.org/search?q=Dunaliella%20salina" title=" Dunaliella salina"> Dunaliella salina</a>, <a href="https://publications.waset.org/search?q=Dunaliella%20bardawil" title=" Dunaliella bardawil"> Dunaliella bardawil</a>, <a href="https://publications.waset.org/search?q=stress%20factors" title=" stress factors"> stress factors</a>, <a href="https://publications.waset.org/search?q=pigments" title=" pigments"> pigments</a>, <a href="https://publications.waset.org/search?q=growth." title=" growth."> growth.</a> </p> <a href="https://publications.waset.org/10008774/the-effect-of-temperature-and-salinity-on-the-growth-and-carotenogenesis-of-three-dunaliella-species-dunaliella-sp-lake-isolate-d-salina-ccap-1918-and-d-bardawil-lb-2538-cultivated-under-laboratory-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10008774/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10008774/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10008774/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10008774/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10008774/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10008774/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10008774/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10008774/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10008774/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10008774/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10008774.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1007</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Surviving Abiotic Stress: The Relationship between High Light and High Salt Tolerance</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Rutanachai%20Thaipratum">Rutanachai Thaipratum</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The mechanism of abiotic stress tolerance is crucial for plants to survive in harsh condition and the knowledge of this mechanism can be use to solve the problem of declining productivity of plants or crops around the world. However in-depth description is still unclear and it is argued, in particular that there is a relationship between high salinity tolerance and the ability to tolerate high light condition. In this study, Dunaliella salina, which can withstand high salt was used as a model. Chlorophyll fluorometer for nonphotochemical quenching (NPQ) measurement and high-performance liquid chromatography for pigment determination was used. The results show that NPQ value and the amount of pigment were increased along with the levels of salinity. However, it establish a clear relationship between high salt and high light but the further study to optimized the solutions mentioned above is still required. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Abiotic%20stress%20tolerance" title="Abiotic stress tolerance">Abiotic stress tolerance</a>, <a href="https://publications.waset.org/search?q=Dunaliella%20salina" title=" Dunaliella salina"> Dunaliella salina</a>, <a href="https://publications.waset.org/search?q=Nonphotochemical%0Aquenching" title=" Nonphotochemical quenching"> Nonphotochemical quenching</a>, <a href="https://publications.waset.org/search?q=Zeaxanthin." title=" Zeaxanthin."> Zeaxanthin.</a> </p> <a href="https://publications.waset.org/3778/surviving-abiotic-stress-the-relationship-between-high-light-and-high-salt-tolerance" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/3778/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/3778/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/3778/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/3778/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/3778/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/3778/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/3778/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/3778/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/3778/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/3778/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/3778.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1636</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Ethyl Methane Sulfonate-Induced Dunaliella salina KU11 Mutants Affected for Growth Rate, Cell Accumulation and Biomass</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Vongsathorn%20Ngampuak">Vongsathorn Ngampuak</a>, <a href="https://publications.waset.org/search?q=Yutachai%20Chookaew"> Yutachai Chookaew</a>, <a href="https://publications.waset.org/search?q=Wipawee%20Dejtisakdi"> Wipawee Dejtisakdi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p><em>Dunaliella salina</em> has great potential as a system for generating commercially valuable products, including beta-carotene, pharmaceuticals, and biofuels. Our goal is to improve this potential by enhancing growth rate and other properties of <em>D. salina</em> under optimal growth conditions. We used ethyl methane sulfonate (EMS) to generate random mutants in <em>D. salina </em>KU11, a strain classified in Thailand. In a preliminary experiment, we first treated <em>D. salina</em> cells with 0%, 0.8%, 1.0%, 1.2%, 1.44% and 1.66% EMS to generate a killing curve. After that, we randomly picked 30 candidates from approximately 300 isolated survivor colonies from the 1.44% EMS treatment (which permitted 30% survival) as an initial test of the mutant screen. Among the 30 survivor lines, we found that 2 strains (mutant #17 and #24) had significantly improved growth rates and cell number accumulation at stationary phase approximately up to 1.8 and 1.45 fold, respectively, 2 strains (mutant #6 and #23) had significantly decreased growth rates and cell number accumulation at stationary phase approximately down to 1.4 and 1.35 fold, respectively, while 26 of 30 lines had similar growth rates compared with the wild type control. We also analyzed cell size for each strain and found there was no significant difference comparing all mutants with the wild type. In addition, mutant #24 had shown an increase of biomass accumulation approximately 1.65 fold compared with the wild type strain on day 5 that was entering early stationary phase. From these preliminary results, it could be feasible to identify <em>D. salina</em> mutants with significant improved growth rate, cell accumulation and biomass production compared to the wild type for the further study; this makes it possible to improve this microorganism as a platform for biotechnology application.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Dunaliella%20salina" title="Dunaliella salina">Dunaliella salina</a>, <a href="https://publications.waset.org/search?q=mutant" title=" mutant"> mutant</a>, <a href="https://publications.waset.org/search?q=ethyl%20methane%20sulfonate" title=" ethyl methane sulfonate"> ethyl methane sulfonate</a>, <a href="https://publications.waset.org/search?q=growth%20rate" title=" growth rate"> growth rate</a>, <a href="https://publications.waset.org/search?q=biomass." title=" biomass."> biomass.</a> </p> <a href="https://publications.waset.org/10004961/ethyl-methane-sulfonate-induced-dunaliella-salina-ku11-mutants-affected-for-growth-rate-cell-accumulation-and-biomass" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10004961/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10004961/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10004961/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10004961/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10004961/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10004961/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10004961/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10004961/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10004961/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10004961/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10004961.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1848</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Codon-optimized Carbonic Anhydrase from Dunaliella species: Expression and Characterization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Seung%20Pil%20Pack">Seung Pil Pack</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Carbonic anhydrases (CAs) has been focused as biological catalysis for CO2 sequestration process because it can catalyze the conversion of CO2 to bicarbonate. Here, codon-optimized sequence of 伪 type-CA cloned from Duneliala species. (DsCAopt) was constructed, expressed, and characterized. The expression level in E. coli BL21(DE3) was better for codon-optimized DsCAopt than intact sequence of DsCAopt. DsCAopt enzyme shows high-stability at pH 7.6/10.0. In final, we demonstrated that in the Ca2+ solution, DsCAopt enzyme can catalyze well the conversion of CO2 to CaCO3, as the calcite form. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Carbonic%20anhydrase" title="Carbonic anhydrase">Carbonic anhydrase</a>, <a href="https://publications.waset.org/search?q=Codon-optimization" title=" Codon-optimization"> Codon-optimization</a>, <a href="https://publications.waset.org/search?q=Duneliala%20species" title=" Duneliala species"> Duneliala species</a>, <a href="https://publications.waset.org/search?q=CO2%20sequestration" title=" CO2 sequestration"> CO2 sequestration</a> </p> <a href="https://publications.waset.org/8093/codon-optimized-carbonic-anhydrase-from-dunaliella-species-expression-and-characterization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/8093/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/8093/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/8093/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/8093/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/8093/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/8093/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/8093/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/8093/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/8093/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/8093/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/8093.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1646</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Oil Extraction from Microalgae Dunalliela sp. by Polar and Non-Polar Solvents</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=A.%20Zonouzi">A. Zonouzi</a>, <a href="https://publications.waset.org/search?q=M.%20Auli"> M. Auli</a>, <a href="https://publications.waset.org/search?q=M.%20Javanmard%20Dakheli"> M. Javanmard Dakheli</a>, <a href="https://publications.waset.org/search?q=M.%20A.%20Hejazi"> M. A. Hejazi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Microalgae are tiny photosynthetic plants. Nowadays, microalgae are being used as nutrient-dense foods and sources of fine chemicals. They have significant amounts of lipid, carotenoids, vitamins, protein, minerals, chlorophyll, and pigments. Oil extraction from algae is a hotly debated topic currently because introducing an efficient method could decrease the process cost. This can determine the sustainability of algae-based foods. Scientific research works show that solvent extraction using chloroform/methanol (2:1) mixture is one of the efficient methods for oil extraction from algal cells, but both methanol and chloroform are toxic solvents, and therefore, the extracted oil will not be suitable for food application. In this paper, the effect of two food grade solvents (hexane and hexane/ isopropanol) on oil extraction yield from microalgae <em>Dunaliella </em>sp. was investigated and the results were compared with chloroform/methanol (2:1) extraction yield. It was observed that the oil extraction yield using hexane, hexane/isopropanol (3:2) and chloroform/methanol (2:1) mixture were 5.4, 13.93, and 17.5 (% w/w, dry basis), respectively. The fatty acid profile derived from GC illustrated that the palmitic (36.62%), oleic (18.62%), and stearic acids (19.08%) form the main portion of fatty acid composition of microalgae <em>Dunalliela </em>sp. oil. It was concluded that, the addition of isopropanol as polar solvent could increase the extraction yield significantly. Isopropanol solves cell wall phospholipids and enhances the release of intercellular lipids, which improves accessing of hexane to fatty acids.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Fatty%20acid%20profile" title="Fatty acid profile">Fatty acid profile</a>, <a href="https://publications.waset.org/search?q=Microalgae" title=" Microalgae"> Microalgae</a>, <a href="https://publications.waset.org/search?q=Oil%20extraction" title=" Oil extraction"> Oil extraction</a>, <a href="https://publications.waset.org/search?q=Polar%20solvent." title=" Polar solvent. 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