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fade-in " data-src="//admeshop.com/cdn/shop/files/DrugInteractionsNEG_14ed349d-b85f-4571-8089-fbb3f5420cec_{width}x.png?v=1702464243" data-widths="[180, 220, 300, 360, 460, 540, 720, 900, 1080, 1296, 1512, 1728, 2048]" data-aspectratio="1.0" data-sizes="auto" alt="" > <noscript> <img class="rimage__image" src="//admeshop.com/cdn/shop/files/DrugInteractionsNEG_14ed349d-b85f-4571-8089-fbb3f5420cec_1024x1024.png?v=1702464243" alt=""> </noscript> </div> </div> </div> </div> </a></div></div> <div class="slideshow-controls"> <div class="slideshow-controls__arrows"></div> </div> <span class="product-label-list"><script id="variant-label-8031076352168-45608853373096" type="text/template"></script><div class="product-label-container"></div></span> </div> <div class="title-row" > <h1 class="title">CYP inhibition, hepatocytes</h1> </div> <div class="" > <div class="product-description rte cf"><span>The test compound is preincubated with human hepatocytes for 0 and 30min. Thereafter, CYP activity is measured by incubating the hepatocytes with a cocktail of CYP-specific substrates (1A2, 2B6, 2C8, 2C9, 2D6, 2C19 and 3A). Direct inhibition is observed from the samples preincubated for 0 min as reduced level of CYP-specific metabolite formation in the incubations compared to solvent samples (IC50 determination). Time-dependent (TDI) inhibition is detected as changes in inhibition curves after preincubation for 0 and 30 min (IC50 shift determination).</span></div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item is-open" open> <summary class="cc-accordion-item__title"><div class="with-icon"> <span class="material-symbols-outlined"> check </span> <span class="with-icon__beside">Protocol</span> </div></summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p>Test concentrations: 6</p><p>Time points: 1</p><p>Replicates: 2</p><p>Positive controls</p><p>UPLC/MS/MS analysis</p></div> </div> </details> </div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item is-open" open> <summary class="cc-accordion-item__title"><div class="with-icon"> <span class="material-symbols-outlined"> check </span> <span class="with-icon__beside">Deliverables</span> </div></summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p><span class="metafield-multi_line_text_field">Direct inhibition as % inhibition or as IC50 for each CYP, tentative Ki values, time-dependent inhibition as IC50 shift or as % inhibition<br /> </span></p></div> </div> </details> </div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item is-open" open> <summary class="cc-accordion-item__title"><div class="with-icon"> <span class="material-symbols-outlined"> check </span> <span class="with-icon__beside">Reporting</span> </div></summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p><span class="metafield-multi_line_text_field">Full Word/PDF report<br /> </span></p></div> </div> </details> </div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item"> <summary class="cc-accordion-item__title">Background</summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p>Inhibition of cytochrome P450 (CYP) enzymes by a new chemical entity (NCE) may decrease the metabolism of co-medicated drugs. We offer assays for individual enzymes as well as a cocktail approach to determine inhibition towards multiple enzymes in the same incubation. Liver microsomes are recommended for high-throughput screenings and for mechanistic approach. In addition, CYP inhibition evaluation can be conducted using pooled hepatocytes, for compounds where hepatocytes are a better suited model.</p></div> </div> </details> </div> </div> </div> </div> <div class="detail product-column-right" data-cc-animate data-cc-animate-delay="0.2s"> <div class="product-form" data-ajax-add-to-cart="true" data-product-id="8031076352168" data-enable-history-state="true"> <div class="buy-buttons-row" > <form method="post" action="/cart/add" id="product-form-template--16460963283112__main-8031076352168" accept-charset="UTF-8" class="form product-purchase-form" enctype="multipart/form-data" data-product-id="8031076352168"><input type="hidden" name="form_type" value="product" /><input type="hidden" name="utf8" value="✓" /> <select name="id" class="original-selector hidden" aria-label="Options"> <option value="45608853373096" selected="selected" data-stock="">Default Title</option> </select> <div class="quantity-submit-row input-row "> <div class="quantity-submit-row__submit input-row"><button class="button button--large" type="submit" data-add-to-cart-text="Add to quote request">Add to quote request</button> </div> </div> <input type="hidden" name="product-id" value="8031076352168" /><input type="hidden" name="section-id" value="template--16460963283112__main" /></form> </div> <div class="lightish-spaced-row-above only-in-quickbuy"> <a class="more" href="/products/cyp-inhibition-hepatocytes"> <span class="beside-svg underline">More details</span> <span class="icon--small icon-natcol ltr-icon"> <span class="material-symbols-outlined"> chevron_right </span></span> </a> </div> </div> <script type="application/json" id="ProductJson-8031076352168">{"id":8031076352168,"title":"CYP inhibition, hepatocytes","handle":"cyp-inhibition-hepatocytes","description":"\u003cspan\u003eThe test compound is preincubated with human hepatocytes for 0 and 30min. 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Thereafter, CYP activity is measured by incubating the hepatocytes with a cocktail of CYP-specific substrates (1A2, 2B6, 2C8, 2C9, 2D6, 2C19 and 3A). Direct inhibition is observed from the samples preincubated for 0 min as reduced level of CYP-specific metabolite formation in the incubations compared to solvent samples (IC50 determination). Time-dependent (TDI) inhibition is detected as changes in inhibition curves after preincubation for 0 and 30 min (IC50 shift determination).\u003c\/span\u003e"}</script> </div> </div> </div> <script type="application/ld+json"> { "@context": "http://schema.org/", "@type": "Product", "name": "CYP inhibition, hepatocytes", "url": "https:\/\/admeshop.com\/products\/cyp-inhibition-hepatocytes","image": [ "https:\/\/admeshop.com\/cdn\/shop\/files\/DrugInteractionsNEG_14ed349d-b85f-4571-8089-fbb3f5420cec_1299x.png?v=1702464243" ],"description": "The test compound is preincubated with human hepatocytes for 0 and 30min. Thereafter, CYP activity is measured by incubating the hepatocytes with a cocktail of CYP-specific substrates (1A2, 2B6, 2C8, 2C9, 2D6, 2C19 and 3A). Direct inhibition is observed from the samples preincubated for 0 min as reduced level of CYP-specific metabolite formation in the incubations compared to solvent samples (IC50 determination). 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