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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><h3> Detection of cancer driver variants and assessment of tumor mutational burden (TMB), an emerging immuno-oncology biomarker, with next-generation sequencing </h3></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>Immune checkpoint inhibitors have changed the treatment paradigm for a variety of cancers, but not all patients will respond to immunotherapies. Relevant biomarkers have shown to be as important as the therapies since they guide in our quest to help match patients with the appropriate treatment.</p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>Our multi-biomarker next-generation sequencing (NGS)-based assays have been adopted by leading cancer institutions to comprehensively profile thousands of clinical research samples (one example is the <a href="/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/analytical-performance-oncomine-nci-match-trial-assay.html">NCI-Match Trial Assay</a>). Given the complexity of the immune response and tumor biology, additional biomarkers are needed to evaluate their associations with response to immuno-oncology (I-O) therapies.  Tumor mutational burden (TMB) is an exciting biomarker in multiple disease types that may further immunotherapy research.</p></div><div class="text parbase section"><p>The Ion Torrent Oncomine Tumor Mutation Load Assay is a robust, targeted next-generation sequencing (NGS) assay designed for tumor profiling by annotation of cancer driver variants and provides accurate quantification of TMB from limited formalin- fixed, paraffin-embedded (FFPE) samples. Our streamlined, built-in analysis solution allows you to confidently detect cancer driver variants and assess tumor mutational burden in ~2.5 days for your research studies.</p><p><a href="#order"><span class="btn btn-featured">Order now</span></a>    <span class="icon icon-pdf"> </span><a href="http://assets.thermofisher.com/TFS-Assets/CSD/Flyers/oncomine-tumor-mutation-load-assay-flyer.pdf" target="_blank">Download flyer ›</a>    <a href="/sg/en/home/global/forms/clinical/immune-repertoire-assay-plus-request-quote.html">Request more info ›</a><br><br></p></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div container-id="cq-colctrl-lt1" comp-id="A1"><div class="heading section"><h3> How can tumor mutational burden (TMB) be accurately measured from FFPE samples? </h3></div></div><div container-id="cq-colctrl-lt1" comp-id="A2"><div class="text parbase section"><p>Learn more by viewing our featured poster from AACR 2019.</p><p><a href="/sg/en/home/global/forms/life-science/tmb-poster-request.html"><b>Download the poster ›</b></a></p></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"><div container-id="cq-colctrl-lt1" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_ad2fuiparimage_827a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.full.high.jpg/1625578290433.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.full.high.jpg/1625578290433.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.320.high.jpg/1625578290433.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.480.high.jpg/1625578290433.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.320.medium.jpg/1625578290433.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.620.high.jpg/1625578290433.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.320.low.jpg/1625578290433.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_ad2f/uipar/image_827a.img.480.medium.jpg/1625578290433.jpg" alt='aacr2019-tmb-ffpe-poster-thumbnail' title='aacr2019-tmb-ffpe-poster-thumbnail'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_ad2fuiparimage_827a img{ width:100%; max-width: 140px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="well-cmp well-mini well-white section"><div class="well well-white well-mini"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h3><a href="/sg/en/home/global/forms/life-science/tumor-mutation-burden-on-demand-webinar.html">Webinar: Determining tumor mutation load using Oncomine Tumor Mutation Load Assay</a></h3><p><b>Speaker:</b> Dr. Jose Carlos Machado, PhD, Ipatimup, Porto, Portugal</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_a071uiparimage_4944"><a href="/sg/en/home/global/forms/life-science/tumor-mutation-burden-on-demand-webinar.html.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.320.low.png/1625578290559.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.320.medium.png/1625578290559.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.480.medium.png/1625578290559.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.620.high.png/1625578290559.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.full.high.png/1625578290559.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.full.high.png/1625578290559.png,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.320.high.png/1625578290559.png,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.480.high.png/1625578290559.png,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.320.medium.png/1625578290559.png,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.620.high.png/1625578290559.png,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.320.low.png/1625578290559.png,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_a071/uipar/image_4944/foregroundimg.img.480.medium.png/1625578290559.png" alt='webinar' title='webinar'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_a071uiparimage_4944 img{ width:100%; max-width: 1272px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_a071uiparimage_4944 .image-contain" + "," + "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_a071uiparimage_4944 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"></div></div><div style="clear:both"></div><div class="heading section"><h3 id="video"> Video: How can the tumor mutation burden be a game changing immuno-oncology biomarker </h3></div><div class="text parbase section"><p><b>Speaker: </b>Dr Wolfram Jochum, Institute of Pathology, Kantonsspital St.Gallen, Switzerland</p></div><div class="brightcoveplayer section"><div data-video="c42ce9487456"><script type="text/javascript"> var loadEME = true; 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div></div></div></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> The next immuno-oncology biomarker </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_9ad6uiparimage_8832"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.full.high.jpg/1625578290731.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.full.high.jpg/1625578290731.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.320.high.jpg/1625578290731.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.480.high.jpg/1625578290731.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.320.medium.jpg/1625578290731.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.620.high.jpg/1625578290731.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.320.low.jpg/1625578290731.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/well_9ad6/uipar/image_8832/foregroundimg.img.480.medium.jpg/1625578290731.jpg" alt='Jose-machado' title='Jose-machado'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_9ad6uiparimage_8832 img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_9ad6uiparimage_8832 .image-contain" + "," + "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsyswell_9ad6uiparimage_8832 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><blockquote style="font-family: tahoma, arial, helvetica, sans-serif; font-size: 12px;"><i>Tumor mutation load may be the next immuno-oncology biomarker to be implemented in our daily routine clinical research. We used the Oncomine Tumor Mutation Load Assay on a retrospective colon cancer cohort, and were able to separate high and low mutation load samples with results correlating well with the MSI status of the tumors. The assay yielded rapid and robust results with its streamlined informatics. Together with other Oncomine assays, we truly have a comprehensive solution for tumor samples.</i></blockquote><p style="font-family: tahoma, arial, helvetica, sans-serif; font-size: 12px;">Prof. José Carlos Machado, PhD<br> Board of Directors member and Group Coordinator<br> Ipatimup, Porto, Portugal</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="heading section"><div class="h2"><br>Faster TMB insights: from sample-to-answer in less than 3 days </div></div><div class="text parbase section"><p>Our streamlined solution allows you to confidently quantify somatic mutations and assess TMB with limited sample amounts.  For maximum throughput, eight samples can be multiplexed per Ion 540 Chip, in manual and automated library preparation.</p></div><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysimage_6d4f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.320.low.jpg/1625578290827.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.320.medium.jpg/1625578290827.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.480.medium.jpg/1625578290827.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.620.high.jpg/1625578290827.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.full.high.jpg/1625578290827.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.320.high.jpg/1625578290827.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.480.high.jpg/1625578290827.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.320.medium.jpg/1625578290827.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.620.high.jpg/1625578290827.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.320.low.jpg/1625578290827.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/image_6d4f/foregroundimg.img.480.medium.jpg/1625578290827.jpg" alt='workflow-Prep-seq-analyse' title='workflow-Prep-seq-analyse'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysimage_6d4f img{ width:100%; 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div><div class="tab-pane" id="container-930624f2-b607-4a21-bca7-792d82fe5eab"><div class="container-930624f2-b607-4a21-bca7-792d82fe5eab tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Analyze your data with the Ion Reporter Software workflow for TMB in w5.10.</b></p><p><b><a href="https://ionreporter.thermofisher.com/ir/">Test drive Ion Reporter Software ›</a></b></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="brightcoveplayer section"><div data-video="520990a5a719"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-520990a5a719', 10); </script></div><div class="video-container video-single"><div id="span-video-520990a5a719" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-520990a5a719" class="brightcove-container"><script>document.createElement('video')</script><video id="video-520990a5a719" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="3947709518001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Ion Reporter Software and Server – Simplify our bioinformatics path to research results", "description": "From human variant detection to microbial diversity, Ion Reporter™ Software provides an optimized suite of simple data analysis tools that streamline Ion PGM™ and Ion Proton™ System data analysis.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/defa94aa-6617-4c20-872d-ccbe5baedc41/5a6abae0-73f6-4d61-a74d-a11ab6df307e/160x90/match/image.jpg"], "uploadDate": "2014-12-17T14:41:19.794Z", "duration": "PT2M41.472S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/3947709518001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=3947709518001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=3947709518001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><script> var navWorkFlow = $('.nav-workflow'), tallestTab = 0; navWorkFlow.find('li').each(function(){ if ($(this).height() > tallestTab) { tallestTab = $(this).height(); } }); navWorkFlow.find('a').css('height',tallestTab); </script><div class="workflow-accordion accordion" id="accordion-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> Step 1 : Prepare </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-046a537d-5e06-42c1-aaac-f48453eee2d9"><div class="container-046a537d-5e06-42c1-aaac-f48453eee2d9 tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Prepare libraries with the Oncomine Tumor Mutation Load Assay, using as little as 20 ng of DNA FFPE input.</b></p><p><b><a href="/order/catalog/product/A37910?SID=srch-srp-A37910">Buy now ›</a></b></p><p><b>Prepare templates manually or with the Ion Chef Instrument for an automated workflow.</b></p><p><b><a href="/sg/en/home/global/forms/ion-chef-system-contact-form.html">Get a quote ›</a></b></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p style="text-align: right;"><span class="icon-time"></span> Total hands-on time: 30 minutes<br></p></div><div class="brightcoveplayer section"><div data-video="224bff574cb4"><script type="text/javascript"> var loadEME = true; 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var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> Step 2 : Sequence </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-11699d1c-2b5b-49aa-a281-2b7f50f243bf"><div class="container-11699d1c-2b5b-49aa-a281-2b7f50f243bf tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Sequence your samples with an Ion GeneStudio S5 Series system and Ion 540 or Ion 550 Chip.</b></p><p><b><a href="/sg/en/home/global/forms/ion-chef-system-contact-form.html">Get a quote ›</a></b></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p style="text-align: right;"><span class="icon-time"></span> Total hands-on time: < 15 minutes<br></p></div><div class="brightcoveplayer section"><div data-video="c10181cfbdf0"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-c10181cfbdf0', 10); </script></div><div class="video-container video-single"><div id="span-video-c10181cfbdf0" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-c10181cfbdf0" class="brightcove-container"><script>document.createElement('video')</script><video id="video-c10181cfbdf0" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="4823596815001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "The Workflow | Ion S5 Next Generation Sequencing System", "description": "Thermo Fisher Scientific recently announced their new sequencer, the Ion S5™ Sequencer. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> Step 3 : Analyze </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-930624f2-b607-4a21-bca7-792d82fe5eab"><div class="container-930624f2-b607-4a21-bca7-792d82fe5eab tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Analyze your data with the Ion Reporter Software workflow for TMB in w5.10.</b></p><p><b><a href="https://ionreporter.thermofisher.com/ir/">Test drive Ion Reporter Software ›</a></b></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="brightcoveplayer section"><div data-video="464dfb4d0fcf"><script type="text/javascript"> var loadEME = true; 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var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="accordion section"><div class="accordion" id="a0209de5e1fd42dfb61f5a21b298096d" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#a0209de5e1fd42dfb61f5a21b298096d" href="#bioinformatics"><span class="caret"></span> A carefully designed bioinformatics workflow analysis solution </a></div><div class="accordion-body collapse " id="bioinformatics"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>The Ion Reporter analysis workflow has been designed to generate results with a tumor-only workflow. Using the Oncomine Variant Annotator Plugin, driver variants are separated from passenger mutations, allowing the user to focus on the relevant few specific to targeted therapies.  A simple TMB readout (Mutations/Mb) is provided in addition to a detailed analysis of mutation signatures, providing meaningful insights for researching the disease etiology (Figure 1).</p></div><div class="text parbase section"><p style="text-align: center;"><img src="/content/dam/LifeTech/global/clinical/images/2017/NewPieChart2.jpg"></p><p><small><b>Figure 1.</b></small></p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#a0209de5e1fd42dfb61f5a21b298096d" href="#assess"><span class="caret"></span> Assessment of tumor mutational burden: WES or targeted sequencing? </a></div><div class="accordion-body collapse " id="assess"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>A targeted NGS panel can replace exome sequencing, bringing the advantage of a robust assay designed for oncology research specimens such as FFPE tissue. The Oncomine Tumor Mutation Load Assay enables accurate measurements of somatic mutations without the need of a matched normal sample. As panels become smaller in size, the zone of uncertainty associated with estimates of TMB expands rapidly (Figure 2A). Additionally, the coefficient of variance increases rapidly when the size of targeted panels is less than 1 Mb (Figure 2B), especially at low mutation loads. The Oncomine Tumor Mutation Load Assay covers a large genomic footprint of 1.7 Mb, encompassing 1.2 Mb of exonic sequence, to enable accurate mutation counts for samples with a range of tumor mutational burden.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_c59c"><a class="image-enlarge-link" data-lightbox="image_c59c" href="/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/_jcr_content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.jpg/1625578291512.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.320.medium.jpg/1625578291485.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.480.high.jpg/1625578291485.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.620.high.jpg/1625578291485.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.full.high.jpg/1625578291485.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.320.high.jpg/1625578291485.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.480.high.jpg/1625578291485.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.320.medium.jpg/1625578291485.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.620.high.jpg/1625578291485.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.320.low.jpg/1625578291485.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_c59c/foregroundimg.img.480.medium.jpg/1625578291485.jpg" alt='Figure 2A' title='Figure 2A'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_c59c img{ width:100%; max-width: 631px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_c59c .image-contain" + "," + "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_c59c .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><small><span class="icon-zoom-in"></span> Click image to enlarge</small><b>Figure 2A.</b> Zone of uncertainty within 2 standard deviations associated with point estimates of mutation counts as a function of panel size. </small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_4837"><a class="image-enlarge-link" data-lightbox="image_4837" href="/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/_jcr_content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.jpg/1625578291567.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.320.medium.jpg/1625578291540.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.480.high.jpg/1625578291540.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.620.high.jpg/1625578291540.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.full.high.jpg/1625578291540.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.320.high.jpg/1625578291540.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.480.high.jpg/1625578291540.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.320.medium.jpg/1625578291540.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.620.high.jpg/1625578291540.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.320.low.jpg/1625578291540.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_e28f/itemParsys/image_4837/foregroundimg.img.480.medium.jpg/1625578291540.jpg" alt='Figure 2B' title='Figure 2B'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_4837 img{ width:100%; max-width: 631px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_4837 .image-contain" + "," + "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_e28fitemParsysimage_4837 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><small><span class="icon-zoom-in"></span> Click image to enlarge</small><B> Figure 2B.</b> Panel size and coefficient of variance is plotted for point estimates of 10, 20 or 100 mutations per Mb.</small></div></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#a0209de5e1fd42dfb61f5a21b298096d" href="#oncominetumor"><span class="caret"></span> Oncomine Tumor Mutation Load Assay performance: high reproducibility </a></div><div class="accordion-body collapse " id="oncominetumor"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><b>High reproducbility</b><br> TMB was measured by running the assay on eight pairs of replicates. The TMB values were highly correlated (Pearson’s R = 0.99) and demonstrated reproducibility across multiple cancer types and sample types, from cell line DNA to FFPE samples (Figure 3A).  The Oncomine Tumor Mutation Load Assay was tested in a series of retrospectively collected colorectal cancer samples that had been typed for microsatellite instability.  As expected, high microsatellite instability samples had significantly higher TMB values than microsatellite stable samples.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_46d2"><a class="image-enlarge-link" data-lightbox="image_46d2" href="/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/_jcr_content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/backgroundimg.img.jpg/1625578291734.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.320.medium.jpg/1625578291697.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.480.high.jpg/1625578291697.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.620.high.jpg/1625578291697.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.full.high.jpg/1625578291697.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.320.high.jpg/1625578291697.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.480.high.jpg/1625578291697.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.320.medium.jpg/1625578291697.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.620.high.jpg/1625578291697.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.320.low.jpg/1625578291697.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_46d2/foregroundimg.img.480.medium.jpg/1625578291697.jpg" alt='Figure 3A' title='Figure 3A'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_46d2 img{ width:100%; max-width: 696px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_46d2 .image-contain" + "," + "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_46d2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><small><span class="icon-zoom-in"></span> Click image to enlarge</small><b>Figure 3A.</b> Reproducibility of the Oncomine Tumor Mutation Load Assay. Cell lines were obtained from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_c58"><a class="image-enlarge-link" data-lightbox="image_c58" href="/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/_jcr_content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/backgroundimg.img.jpg/1625578291802.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.320.medium.jpg/1625578291754.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.480.high.jpg/1625578291754.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.620.high.jpg/1625578291754.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.full.high.jpg/1625578291754.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.320.high.jpg/1625578291754.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.480.high.jpg/1625578291754.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.320.medium.jpg/1625578291754.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.620.high.jpg/1625578291754.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.320.low.jpg/1625578291754.jpg,https://www.thermofisher.com/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/accordion_4a50/itemspar/accordionitem_401a/itemParsys/image_c58/foregroundimg.img.480.medium.jpg/1625578291754.jpg" alt='Figure 3B' title='Figure 3B'></picture><style> #cq-image-jsp-3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_c58 img{ width:100%; max-width: 696px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_c58 .image-contain" + "," + "#cq-image-jsp-"+"3a2bf50b-7ce6-4eab-86be-b495cf3b4336MainParsysaccordion_4a50itemsparaccordionitem_401aitemParsysimage_c58 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><small><span class="icon-zoom-in"></span> Click image to enlarge</small><b>Figure 3B.</b> Stratification of MSI positive and MSI negative samples with the Oncomine Tumor Mutation Load Assay. MSI status determined by orthogonal method. Data courtesy of Ipatimup.</small></div></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#a0209de5e1fd42dfb61f5a21b298096d').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#a0209de5e1fd42dfb61f5a21b298096d').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><p><b><a href="/sg/en/home/products-and-services/promotions/life-science/next-generation-immuno-oncology-solutions.html">Learn more about our multi-dimensional approach to immuno-oncology ›</a></b><br><b><a href="/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-cancer-research-panel-workflow.html">Learn more about our Oncomine oncology assays for comprehensive genomic profiling ›</a></b></p></div><div class="productlist parbase section"><div id="skulist" style="display:none">A37909, A37910</div><div id='ltajax-4918451445260644858'></div><script type="text/javascript">(function() {$('#ltajax-4918451445260644858').load('/sg/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology/oncomine-tumor-mutation-load-assay/jcr:content/MainParsys/productlist_2b58.ajax.html?skulist=');})(); </script></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2> Resources </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="http://assets.thermofisher.com/TFS-Assets/CSD/posters/association-of-msi-and-tmb-poster.pdf" class="headline"><span class="icon icon-pdf"></span> Poster: Association of MSI and TMB</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/CSD/posters/2018-sitc-poster-conroy-tmb-ring-study.pdf" class="headline"><span class="icon icon-pdf"></span> Poster: Tumor mutational burden (TMB) ring study: Comparison of multiple targeted next-generation sequencing (NGS) platforms</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/CSD/posters/aacr2019-tmb-deamination-ffpe-poster.pdf" class="headline"><span class="icon icon-pdf"></span> Poster: Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNA</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/brochures/sn-000016-da-gallery-enzyme-assays-sn000016-en.pdf" class="headline"><span class="icon icon-pdf"></span> Smart Note: What are Enzyme Assays?</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/brochures/sn-000017-da-gallery-enzyme-master-assays-sn000017-en.pdf" class="headline"><span class="icon icon-pdf"></span> Smart Note: What are the Top 5 Reasons to Consider Fully-automated Enzyme Assays?</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/brochures/in-73823-da-gallery-enzyme-enzyme-master-analyzer-in73823-en.pdf" class="headline"><span class="icon icon-pdf"></span> Infographics: Fully Automated Enzyme Analysis</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/brochures/br-73822-da-gallery-enzyme-enzyme-master-analyzer-br73822-en.pdf" class="headline"><span class="icon icon-pdf"></span> Brochure: Enzyme Assay Automation for Walkaway Efficiency—Gallery Enzyme Master Enzyme Analyzer</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="http://assets.thermofisher.com/TFS-Assets/CSD/posters/tumor-mutation-burden-somatic-mutation-profiling-poster.pdf" class="headline"><span class="icon icon-pdf"></span> Poster: Tumor mutation burden estimation and somatic mutation profiling</a></li><li><a href="/sg/en/home/global/forms/life-science/tmb-poster-request.html" class="headline">Poster: TMB and Genomic Profiling from FFPE Research Samples Using a Targeted NGS Assay</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div><div class="MainInheritParsys iparsys parsys"><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="text_72be text parbase"><p>For Research Use Only. 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