TY - JFULL AU - M.V.R.K Sarma and Krishna Saharan and Lalit Kumar and Ashwani Gautam and Avhijeet Kapoor and Nishant Srivastava and Vikram Sahai and V.S Bisaria PY - 2010/6/ TI - Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81 T2 - International Journal of Biomedical and Biological Engineering SP - 387 EP - 392 VL - 4 SN - 1307-6892 UR - https://publications.waset.org/pdf/6767 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 41, 2010 N2 - The fluorescent pseudomonad strain R81 is a root colonizing rhizobacteria which promotes the growth of many plants by various mechanisms. Its broth containing siderophore (ironchelating compound) and 2,4- diacetyl phloroglucinol (DAPG) is used for preparing bioinoculant formulations for agronomical applications. Glycerol was found to be the best carbon source for improved biomass production. Splitting of nitrogen source to NH4Cl and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan at 0.5 % in the medium increased the siderophore production to 850 mg/l. During batch cultivation of the strain in a bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of siderophore and 20 mg/l of DAPG was achieved when glycerol was 15 g/l and C/N ratio was maintained at 12.5. In case of intermittent feeding of fresh medium during fed-batch cultivation, the dry cell mass was increased to 25 g/l with improved production of DAPG to 70 mg/l. ER -