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aneuploidy</title> <meta name="description" content="Search results for: aneuploidy"> <meta name="keywords" content="aneuploidy"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="aneuploidy"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 9</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: aneuploidy</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> The Prevalence of X-Chromosome Aneuploidy in Recurrent Pregnancy Loss</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rim%20Frikha">Rim Frikha</a>, <a href="https://publications.waset.org/abstracts/search?q=Nouha%20Bouayed"> Nouha Bouayed</a>, <a href="https://publications.waset.org/abstracts/search?q=Afifa%20Sellami"> Afifa Sellami</a>, <a href="https://publications.waset.org/abstracts/search?q=Nozha%20Chakroun"> Nozha Chakroun</a>, <a href="https://publications.waset.org/abstracts/search?q=Salima%20Douad"> Salima Douad</a>, <a href="https://publications.waset.org/abstracts/search?q=Leila%20Keskes"> Leila Keskes</a>, <a href="https://publications.waset.org/abstracts/search?q=Tarek%20Rebai"> Tarek Rebai</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recurrent pregnancy loss (RPL), classically defined as the occurrence of two or more failed pregnancies, is a serious reproductive problem, in which, chromosomal rearrangements in either carrier are a major cause; mainly the chromosome aneuploidy. This study was conducted to determine the frequency and contribution of X-chromosome aneuploidy in recurrent pregnancy loss. A retrospective study was carried out among 100 couples with more than 2 miscarriages, referred to our genetic counseling. In all the cases the detailed reproductive histories were taken. Chromosomal analysis was performed using RHG banding in peripheral blood. Of a total of 100 couples; 3 patients with a detected X-chromosome aneuploidy were identified with an overall frequency of 3%. Chromosome abnormalities are as below: a Turner syndrome with 45, X/46, XX mosaicism, a 47, XXX, and a Klinefelter syndrome with 46, XY/47, XXY. These data show a high incidence of X-chromosome aneuploidy; mainly with mosaicism; in RPL. Thus, couples with such chromosomal abnormality should be referred to a clinical geneticist with whom the option of pre-implantation genetic diagnosis in subsequent pregnancy should be discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aneuploidy" title="aneuploidy">aneuploidy</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20testing" title=" genetic testing"> genetic testing</a>, <a href="https://publications.waset.org/abstracts/search?q=recurrent%20pregnancy%20loss" title="recurrent pregnancy loss">recurrent pregnancy loss</a>, <a href="https://publications.waset.org/abstracts/search?q=X-chromosome" title=" X-chromosome"> X-chromosome</a> </p> <a href="https://publications.waset.org/abstracts/45376/the-prevalence-of-x-chromosome-aneuploidy-in-recurrent-pregnancy-loss" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45376.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">360</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Assessment of Sperm Aneuploidy Using Advanced Sperm Fish Technique in Infertile Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Archana%20S.">Archana S.</a>, <a href="https://publications.waset.org/abstracts/search?q=Usha%20Rani%20G."> Usha Rani G.</a>, <a href="https://publications.waset.org/abstracts/search?q=Anand%20Balakrishnan"> Anand Balakrishnan</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanjana%20R."> Sanjana R.</a>, <a href="https://publications.waset.org/abstracts/search?q=Solomon%20F."> Solomon F.</a>, <a href="https://publications.waset.org/abstracts/search?q=Vijayalakshmi%20J."> Vijayalakshmi J.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: There is evidence that male factors contribute to the infertility of up to 50% of couples, who are evaluated and treated for infertility using advanced assisted reproductive technologies. Genetic abnormalities, including sperm chromosome aneuploidy as well as structural aberrations, are one of the major causes of male infertility. Recent advances in technology expedite the evaluation of sperm aneuploidy. The purpose of the study was to de-termine the prevalence of sperm aneuploidy in infertile males and the degree of association between DNA fragmentation and sperm aneuploidy. Methods: In this study, 75 infertile men were included, and they were divided into four abnormal groups (Oligospermia, Terato-spermia, Asthenospermia and Oligoasthenoteratospermia (OAT)). Men with children who were normozoospermia served as the control group. The Fluorescence in situ hybridization (FISH) method was used to test for sperm aneuploidy, and the Sperm Chromatin Dispersion Assay (SCDA) was used to measure the fragmentation of sperm DNA. Spearman's correla-tion coefficient was used to evaluate the relationship between sperm aneuploidy and sperm DNA fragmentation along with age. P < 0.05 was regarded as significant. Results: 75 partic-ipants' ages varied from 28 to 48 years old (35.5±5.1). The percentage of spermatozoa bear-ing X and Y was determined to be statistically significant (p-value < 0.05) and was found to be 48.92% and 51.18% of CEP X X 1 – nucish (CEP XX 1) [100] and CEP Y X 1 – nucish (CEP Y X 1) [100]. When compared to the rate of DNA fragmentation, it was discovered that infertile males had a greater frequency of sperm aneuploidy. Asthenospermia and OAT groups in sex chromosomal aneuploidy were significantly correlated (p<0.05). Conclusion: Sperm FISH and SCDA assay results showed increased sperm aneuploidy frequency, and DNA fragmentation index in infertile men compared with fertile men. There is a significant relationship observed between sperm aneuploidy and DNA fragmentation in OAT patients. When evaluating male variables and idiopathic infertility, the sperm FISH screening method can be used as a valuable diagnostic tool. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ale%20infertility" title="ale infertility">ale infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=dfi%20%28dna%20fragmentation%20assay%29%20%28scd-sperm%20chromatin%20dispersion%29.art%20%28artificial%20reproductive%20technology%29" title=" dfi (dna fragmentation assay) (scd-sperm chromatin dispersion).art (artificial reproductive technology)"> dfi (dna fragmentation assay) (scd-sperm chromatin dispersion).art (artificial reproductive technology)</a>, <a href="https://publications.waset.org/abstracts/search?q=trisomy" title=" trisomy"> trisomy</a>, <a href="https://publications.waset.org/abstracts/search?q=aneuploidy" title=" aneuploidy"> aneuploidy</a>, <a href="https://publications.waset.org/abstracts/search?q=fish%20%28fluorescence%20in-situ%20hybridization%29" title=" fish (fluorescence in-situ hybridization)"> fish (fluorescence in-situ hybridization)</a>, <a href="https://publications.waset.org/abstracts/search?q=oat%20%28oligoasthoteratospermia%29" title=" oat (oligoasthoteratospermia)"> oat (oligoasthoteratospermia)</a> </p> <a href="https://publications.waset.org/abstracts/176566/assessment-of-sperm-aneuploidy-using-advanced-sperm-fish-technique-in-infertile-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/176566.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">54</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Efficacy of Preimplantation Genetic Screening in Women with a Spontaneous Abortion History with Eukaryotic or Aneuploidy Abortus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jayeon%20Kim">Jayeon Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Eunjung%20Yu"> Eunjung Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Taeki%20Yoon"> Taeki Yoon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Most spontaneous miscarriage is believed to be a consequence of embryo aneuploidies. Transferring eukaryotic embryos selected by PGS is expected to decrease the miscarriage rate. Current PGS indications include advanced maternal age, recurrent pregnancy loss, repeated implantation failure. Recently, use of PGS for healthy women without above indications for the purpose of improving in vitro fertilization (IVF) outcomes is on the rise. However, it is still controversy about the beneficial effect of PGS in this population, especially, in women with a history of no more than 2 miscarriages or miscarriage of eukaryotic abortus. This study aimed to investigate if karyotyping result of abortus is a good indicator of preimplantation genetic screening (PGS) in subsequent IVF cycle in women with a history of spontaneous abortion. A single-center retrospective cohort study was performed. Women who had spontaneous abortion(s) (less than 3) and dilatation and evacuation, and subsequent IVF from January 2016 to November 2016 were included. Their medical information was extracted from the charts. Clinical pregnancy was defined as presence of a gestational sac with fetal heart beat detected on ultrasound in week 7. Statistical analysis was performed using SPSS software. Total 234 women were included. 121 out of 234 (51.7%) underwent karyotyping of the abortus, and 113 did not have the abortus karyotyped. Embryo biopsy was performed on 3 or 5 days after oocyte retrieval, followed by embryo transfer (ET) on a fresh or frozen cycle. The biopsied materials were subjected to microarray comparative genomic hybridization. Clinical pregnancy rate per ET was compared between PGS and non-PGS group in each study group. Patients were grouped by two criteria: karyotype of the abortus from previous miscarriage (unknown fetal karyotype (n=89, Group 1), eukaryotic abortus (n=36, Group 2) or aneuploidy abortus (n=67, Group 3)), and pursuing PGS in subsequent IVF cycle (pursuing PGS (PGS group, n=105) or not pursuing PGS (non-PGS group, n=87)). The PGS group was significantly older and had higher number of retrieved oocytes and prior miscarriages compared to non-PGS group. There were no differences in BMI and AMH level between those two groups. In PGS group, the mean number of transferable embryos (eukaryotic embryo) was 1.3 ± 0.7, 1.5 ± 0.5 and 1.4 ± 0.5, respectively (p = 0.049). In 42 cases, ET was cancelled because all embryos biopsied turned out to be abnormal. In all three groups (group 1, 2, and 3), clinical pregnancy rates were not statistically different between PGS and non-PGS group (Group 1: 48.8% vs. 52.2% (p=0.858), Group 2: 70% vs. 73.1% (p=0.730), Group 3: 42.3% vs. 46.7% (p=0.640), in PGS and non-PGS group, respectively). In both groups who had miscarriage with eukaryotic and aneuploidy abortus, the clinical pregnancy rate between IVF cycles with and without PGS was not different. When we compare miscarriage and ongoing pregnancy rate, there were no significant differences between PGS and non-PGS group in all three groups. Our results show that the routine application of PGS in women who had less than 3 miscarriages would not be beneficial, even in cases that previous miscarriage had been caused by fetal aneuploidy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=preimplantation%20genetic%20diagnosis" title="preimplantation genetic diagnosis">preimplantation genetic diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=miscarriage" title=" miscarriage"> miscarriage</a>, <a href="https://publications.waset.org/abstracts/search?q=kpryotyping" title=" kpryotyping"> kpryotyping</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20fertilization" title=" in vitro fertilization"> in vitro fertilization</a> </p> <a href="https://publications.waset.org/abstracts/75730/efficacy-of-preimplantation-genetic-screening-in-women-with-a-spontaneous-abortion-history-with-eukaryotic-or-aneuploidy-abortus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75730.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">181</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Embryonic Aneuploidy – Morphokinetic Behaviors as a Potential Diagnostic Biomarker</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Banafsheh%20Nikmehr">Banafsheh Nikmehr</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohsen%20Bahrami"> Mohsen Bahrami</a>, <a href="https://publications.waset.org/abstracts/search?q=Yueqiang%20Song"> Yueqiang Song</a>, <a href="https://publications.waset.org/abstracts/search?q=Anuradha%20Koduru"> Anuradha Koduru</a>, <a href="https://publications.waset.org/abstracts/search?q=Ayse%20K.%20Vuruskan"> Ayse K. Vuruskan</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongkun%20Lu"> Hongkun Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Mallory%20Pitts"> Mallory Pitts</a>, <a href="https://publications.waset.org/abstracts/search?q=Tolga%20B.%20Mesen"> Tolga B. Mesen</a>, <a href="https://publications.waset.org/abstracts/search?q=Tamer%20M.%20Yalcinkaya"> Tamer M. Yalcinkaya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The number of people who receive in vitro fertilization (IVF) treatment has increased on a startling trajectory over the past two decades. Despite advances in this field, particularly the introduction of intracytoplasmic sperm injection (ICSI) and the preimplantation genetic screening (PGS), the IVF success remains low. A major factor contributing to IVF failure is embryonic aneuploidy (abnormal chromosome content), which often results in miscarriage and birth defects. Although PGS is often used as the standard diagnostic tool to identify aneuploid embryos, it is an invasive approach that could affect the embryo development, and yet inaccessible to many patients due its high costs. As such, there is a clear need for a non-invasive cost-effective approach to identify euploid embryos for single embryo transfer (SET). The reported differences between morphokinetic behaviors of aneuploid and euploid embryos has shown promise to address this need. However, current literature is inconclusive and further research is urgently needed to translate current findings into clinical diagnostics. In this ongoing study, we found significant differences between morphokinetic behaviors of euploid and aneuploid embryos that provides important insights and reaffirms the promise of such behaviors for developing non-invasive methodologies. Methodology—A total of 242 embryos (euploid: 149, aneuploid: 93) from 74 patients who underwent IVF treatment in Carolinas Fertility Clinics in Winston-Salem, NC, were analyzed. All embryos were incubated in an EmbryoScope incubator. The patients were randomly selected from January 2019 to June 2021 with most patients having both euploid and aneuploid embryos. All embryos reached the blastocyst stage and had known PGS outcomes. The ploidy assessment was done by a third-party testing laboratory on day 5-7 embryo biopsies. The morphokinetic variables of each embryo were measured by the EmbryoViewer software (Uniesense FertiliTech) on time-lapse images using 7 focal depths. We compared the time to: pronuclei fading (tPNf), division to 2,3,…,9 cells (t2, t3,…,t9), start of embryo compaction (tSC), Morula formation (tM), start of blastocyst formation (tSC), blastocyst formation (tB), and blastocyst expansion (tEB), as well as intervals between them (e.g., c23 = t3 – t2). We used a mixed regression method for our statistical analyses to account for the correlation between multiple embryos per patient. Major Findings— The average age of the patients was 35.04 yrs. The average patient age associated with euploid and aneuploid embryos was not different (P = 0.6454). We found a significant difference in c45 = t5-t4 (P = 0.0298). Our results indicated this interval on average lasts significantly longer for aneuploid embryos - c45(aneuploid) = 11.93hr vs c45(euploid) = 7.97hr. In a separate analysis limited to embryos from the same patients (patients = 47, total embryos=200, euploid=112, aneuploid=88), we obtained the same results (P = 0.0316). The statistical power for this analysis exceeded 87%. No other variable was different between the two groups. Conclusion— Our results demonstrate the importance of morphokinetic variables as potential biomarkers that could aid in non-invasively characterizing euploid and aneuploid embryos. We seek to study a larger population of embryos and incorporate the embryo quality in future studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=IVF" title="IVF">IVF</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo" title=" embryo"> embryo</a>, <a href="https://publications.waset.org/abstracts/search?q=euploidy" title=" euploidy"> euploidy</a>, <a href="https://publications.waset.org/abstracts/search?q=aneuploidy" title=" aneuploidy"> aneuploidy</a>, <a href="https://publications.waset.org/abstracts/search?q=morphokinteic" title=" morphokinteic"> morphokinteic</a> </p> <a href="https://publications.waset.org/abstracts/156024/embryonic-aneuploidy-morphokinetic-behaviors-as-a-potential-diagnostic-biomarker" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156024.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">88</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Non-Invasive Pre-Implantation Genetic Assessment Using NGS in IVF Clinical Routine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Katalin%20Gombos">Katalin Gombos</a>, <a href="https://publications.waset.org/abstracts/search?q=Bence%20G%C3%A1lik"> Bence Gálik</a>, <a href="https://publications.waset.org/abstracts/search?q=Krisztina%20Ildik%C3%B3%20Kal%C3%A1cs"> Krisztina Ildikó Kalács</a>, <a href="https://publications.waset.org/abstracts/search?q=Krisztina%20G%C3%B6d%C3%B6ny"> Krisztina Gödöny</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%81kos%20V%C3%A1rnagy"> Ákos Várnagy</a>, <a href="https://publications.waset.org/abstracts/search?q=J%C3%B3zsef%20B%C3%B3dis"> József Bódis</a>, <a href="https://publications.waset.org/abstracts/search?q=Attila%20Gyenesei"> Attila Gyenesei</a>, <a href="https://publications.waset.org/abstracts/search?q=G%C3%A1bor%20L.%20Kov%C3%A1cs"> Gábor L. Kovács</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Although non-invasive pre-implantation genetic testing for aneuploidy (NIPGT-A) is potentially appropriate to assess chromosomal ploidy of the embryo, practical application of it in a routine IVF center has not been started in the absence of a recommendation. We developed a comprehensive workflow for a clinically applicable strategy for NIPGT-A based on next-generation sequencing (NGS) technology. We performed MALBAC whole genome amplification and NGS on spent blastocyst culture media of Day 3 embryos fertilized with intra-cytoplasmic sperm injection (ICSI). Spent embryonic culture media of morphologically good quality score embryos were enrolled in further analysis with the blank culture media as background control. Chromosomal abnormalities were identified by an optimized bioinformatics pipeline applying a copy number variation (CNV) detecting algorithm. We demonstrate a comprehensive workflow covering both wet- and dry-lab procedures supporting a clinically applicable strategy for NIPGT-A. It can be carried out within 48 h which is critical for the same-cycle blastocyst transfer, but also suitable for “freeze all” and “elective frozen embryo” strategies. The described integrated approach of non-invasive evaluation of embryonic DNA content of the culture media can potentially supplement existing pre-implantation genetic screening methods. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=next%20generation%20sequencing" title="next generation sequencing">next generation sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20fertilization" title=" in vitro fertilization"> in vitro fertilization</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo%20assessment" title=" embryo assessment"> embryo assessment</a>, <a href="https://publications.waset.org/abstracts/search?q=non-invasive%20pre-implantation%20genetic%20testing" title=" non-invasive pre-implantation genetic testing"> non-invasive pre-implantation genetic testing</a> </p> <a href="https://publications.waset.org/abstracts/143714/non-invasive-pre-implantation-genetic-assessment-using-ngs-in-ivf-clinical-routine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143714.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">156</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Clinical Outcome after in Vitro Fertilization in Women Aged 40 Years and Above: Reasonable Cut-Off Age for Successful Pregnancy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eun%20Jeong%20Yu">Eun Jeong Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Inn%20Soo%20Kang"> Inn Soo Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=Tae%20Ki%20Yoon"> Tae Ki Yoon</a>, <a href="https://publications.waset.org/abstracts/search?q=Mi%20Kyoung%20Koong"> Mi Kyoung Koong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Advanced female age is associated with higher cycle cancelation rates, lower clinical pregnancy rate, increased miscarriage and aneuploidy rates in IVF (In Vitro Fertilization) cycles. This retrospective cohort study was conducted at a Cha Fertility Center, Seoul Station. All fresh non-donor IVF cycles performed in women aged 40 years and above from January 2016 to December 2016 were reviewed. Donor/recipient treatment, PGD/PGS (Preimplantation Genetic Diagnosis/ Preimplantation Genetic Screening) were excluded from analysis. Of the 1,166 cycles from 753 women who completed ovulation induction, 1,047 were appropriate for the evaluation according to inclusion and exclusion criterion. IVF cycles were categorized according to age and grouped into the following 1-year age groups: 40, 41, 42, 43, 44, 45 and > 46. The mean age of patients was 42.4 ± 1.8 years. The median AMH (Anti-Mullerian Hormone) level was 1.2 ± 1.5 ng/mL. The mean number of retrieved oocytes was 4.9 ± 4.3. The clinical pregnancy rate and live birth rate in women > 40 years significantly decreased with each year of advancing age (p < 0.001). The clinical pregnancy rate decreased from 21% at the age of 40 years to 0% at ages above 45 years. Live birth rate decreased from 12.3% to 0%, respectively. There were no clinical pregnancy outcomes among 95 patients aged above 45 years of age. The overall miscarriage rate was 40.7% (range, 36.7%-70%). The transfer of at least one good quality embryo was associated with about 4-9% increased chance of a clinical pregnancy rate. Therefore, IVF in old age women less than 46 had a reasonable chance for successful pregnancy outcomes especially when good quality embryo is transferred. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=advanced%20maternal%20age" title="advanced maternal age">advanced maternal age</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20fertilization" title=" in vitro fertilization"> in vitro fertilization</a>, <a href="https://publications.waset.org/abstracts/search?q=pregnancy%20rate" title=" pregnancy rate"> pregnancy rate</a>, <a href="https://publications.waset.org/abstracts/search?q=live%20birth%20rate" title=" live birth rate"> live birth rate</a> </p> <a href="https://publications.waset.org/abstracts/95316/clinical-outcome-after-in-vitro-fertilization-in-women-aged-40-years-and-above-reasonable-cut-off-age-for-successful-pregnancy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/95316.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">144</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Qf-Pcr as a Rapid Technique for Routine Prenatal Diagnosis of Fetal Aneuploidies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20H.%20Atef">S. H. Atef </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The most common chromosomal abnormalities identified at birth are aneuploidies of chromosome 21, 18, 13, X and Y. Prenatal diagnosis of fetal aneuploidies is routinely done by traditional cytogenetic culture, a major drawback of this technique is the long period of time required to reach a diagnosis. In this study, we evaluated the QF-PCR as a rapid technique for prenatal diagnosis of common aneuploidies. Method:This work was carried out on Sixty amniotic fluid samples taken from patients with one or more of the following indications: Advanced maternal age (3 case), abnormal biochemical markers (6 cases), abnormal ultrasound (12 cases) or previous history of abnormal child (39 cases).Each sample was tested by QF-PCR and traditional cytogenetic. Aneuploidy screenings were performed amplifying four STRs on chromosomes 21, 18, 13, two pseudoautosomal,one X linked, as well as the AMXY and SRY; markers were distributed in two multiplex QFPCR assays (S1 and S2) in order to reduce the risk of sample mishandling. Results: All the QF-PCR results were successful, while there was two culture failures, only one of them was repeated. No discrepancy was seen between the results of both techniques. Fifty six samples showed normal patterns, three sample showed trisomy 21, successfully detected by both techniques and one sample showed normal pattern by QF-PCR but could not be compared to the cytogenetics due to culture failure, the pregnancy outcome of this case was a normal baby. Conclusion: Our study concluded that QF-PCR is a reliable technique for prenatal diagnosis of the common chromosomal aneuploidies. It has the advantages over the cytogenetic culture of being faster with the results appearing within 24-48 hours, simpler, doesn't need a highly qualified staff, less prone to failure and more cost effective. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=QF-PCR" title="QF-PCR">QF-PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=traditional%20cytogenetic%20fetal%20aneuploidies" title=" traditional cytogenetic fetal aneuploidies"> traditional cytogenetic fetal aneuploidies</a>, <a href="https://publications.waset.org/abstracts/search?q=trisomy%2021" title=" trisomy 21"> trisomy 21</a>, <a href="https://publications.waset.org/abstracts/search?q=prenatal%20diagnosis" title=" prenatal diagnosis "> prenatal diagnosis </a> </p> <a href="https://publications.waset.org/abstracts/36049/qf-pcr-as-a-rapid-technique-for-routine-prenatal-diagnosis-of-fetal-aneuploidies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/36049.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">417</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Enhancing Mitochondrial Activity and Metabolism in Aging Female Germ Cells: Synergistic Effects of Dual ROCK and ROS Inhibition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kuan-Hao%20Tsui">Kuan-Hao Tsui</a>, <a href="https://publications.waset.org/abstracts/search?q=Li-Te%20Lin"> Li-Te Lin</a>, <a href="https://publications.waset.org/abstracts/search?q=Chia-Jung%20Li"> Chia-Jung Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The combination of Y-27632 and Vitamin C significantly enhances the quality of aging germ cells by reducing reactive oxygen species (ROS) production, restoring mitochondrial membrane potential balance, and promoting mitochondrial fusion. The age-related decline in oocyte quality contributes to reduced fertility, increased aneuploidy, and diminished embryo quality, with mitochondrial dysfunction in both oocytes and granulosa cells being a key factor in this decline. Experiments on aging germ cells investigated the effects of the Y-27632 and Vitamin C combination. In vivo studies involved aged mice to assess oocyte maturation and ROS accumulation during culture. The assessment included mitochondrial activity, ROS levels, mitochondrial membrane potential, and mitochondrial dynamics. Cellular energy metabolism and ATP production were also measured. The combination treatment effectively addressed mitochondrial dysfunction and regulated cellular energy metabolism, promoting oxygen respiration and increasing ATP production. In aged mice, this supplement treatment enhanced in vitro oocyte maturation and prevented ROS accumulation in aging oocytes during culture. While these findings are promising, further research is needed to explore the long-term effects and potential side effects of the Y-27632 and Vitamin C combination. Additionally, translating these findings to human subjects requires careful consideration. Overall, the study suggests that the Y-27632 and Vitamin C combination could be a promising intervention to mitigate aging-related dysfunction in germ cells, potentially enhancing oocyte quality, particularly in the context of in vitro fertilization. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ovarian%20aging" title="ovarian aging">ovarian aging</a>, <a href="https://publications.waset.org/abstracts/search?q=supplements" title=" supplements"> supplements</a>, <a href="https://publications.waset.org/abstracts/search?q=ROS" title=" ROS"> ROS</a>, <a href="https://publications.waset.org/abstracts/search?q=mitochondria" title=" mitochondria"> mitochondria</a> </p> <a href="https://publications.waset.org/abstracts/186480/enhancing-mitochondrial-activity-and-metabolism-in-aging-female-germ-cells-synergistic-effects-of-dual-rock-and-ros-inhibition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186480.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">40</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Prediction of Live Birth in a Matched Cohort of Elective Single Embryo Transfers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohsen%20Bahrami">Mohsen Bahrami</a>, <a href="https://publications.waset.org/abstracts/search?q=Banafsheh%20Nikmehr"> Banafsheh Nikmehr</a>, <a href="https://publications.waset.org/abstracts/search?q=Yueqiang%20Song"> Yueqiang Song</a>, <a href="https://publications.waset.org/abstracts/search?q=Anuradha%20Koduru"> Anuradha Koduru</a>, <a href="https://publications.waset.org/abstracts/search?q=Ayse%20K.%20Vuruskan"> Ayse K. Vuruskan</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongkun%20Lu"> Hongkun Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Tamer%20M.%20Yalcinkaya"> Tamer M. Yalcinkaya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recent years, we have witnessed an explosion of studies aimed at using a combination of artificial intelligence (AI) and time-lapse imaging data on embryos to improve IVF outcomes. However, despite promising results, no study has used a matched cohort of transferred embryos which only differ in pregnancy outcome, i.e., embryos from a single clinic which are similar in parameters, such as: morphokinetic condition, patient age, and overall clinic and lab performance. Here, we used time-lapse data on embryos with known pregnancy outcomes to see if the rich spatiotemporal information embedded in this data would allow the prediction of the pregnancy outcome regardless of such critical parameters. Methodology—We did a retrospective analysis of time-lapse data from our IVF clinic utilizing Embryoscope 100% of the time for embryo culture to blastocyst stage with known clinical outcomes, including live birth vs nonpregnant (embryos with spontaneous abortion outcomes were excluded). We used time-lapse data from 200 elective single transfer embryos randomly selected from January 2019 to June 2021. Our sample included 100 embryos in each group with no significant difference in patient age (P=0.9550) and morphokinetic scores (P=0.4032). Data from all patients were combined to make a 4th order tensor, and feature extraction were subsequently carried out by a tensor decomposition methodology. The features were then used in a machine learning classifier to classify the two groups. Major Findings—The performance of the model was evaluated using 100 random subsampling cross validation (train (80%) - test (20%)). The prediction accuracy, averaged across 100 permutations, exceeded 80%. We also did a random grouping analysis, in which labels (live birth, nonpregnant) were randomly assigned to embryos, which yielded 50% accuracy. Conclusion—The high accuracy in the main analysis and the low accuracy in random grouping analysis suggest a consistent spatiotemporal pattern which is associated with pregnancy outcomes, regardless of patient age and embryo morphokinetic condition, and beyond already known parameters, such as: early cleavage or early blastulation. Despite small samples size, this ongoing analysis is the first to show the potential of AI methods in capturing the complex morphokinetic changes embedded in embryo time-lapse data, which contribute to successful pregnancy outcomes, regardless of already known parameters. The results on a larger sample size with complementary analysis on prediction of other key outcomes, such as: euploidy and aneuploidy of embryos will be presented at the meeting. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=IVF" title="IVF">IVF</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo" title=" embryo"> embryo</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=time-lapse%20imaging%20data" title=" time-lapse imaging data"> time-lapse imaging data</a> </p> <a href="https://publications.waset.org/abstracts/156028/prediction-of-live-birth-in-a-matched-cohort-of-elective-single-embryo-transfers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156028.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">92</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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