CINXE.COM

<!DOCTYPE html> <html lang="en-US"> <head > <meta charset="UTF-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <meta name='robots' content='index, follow, max-image-preview:large, max-snippet:-1, max-video-preview:-1' />  <title>Engineer Your Protein Using Directed Evolution Methods - Behind the Bench</title> <link rel="canonical" href="https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/" /> <meta property="og:locale" content="en_US" /> <meta property="og:type" content="article" /> <meta property="og:title" content="Engineer Your Protein Using Directed Evolution Methods" /> <meta property="og:description" content="Would you like to evolve your protein of interest but are tired of error-prone PCR approaches? Libraries based on synthetic templates have many advantages and could be the solution for you. Synthetic biology has now paved the way to create genes and libraries that require no natural template at all—almost any design vision is possible." /> <meta property="og:url" content="https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/" /> <meta property="og:site_name" content="Behind the Bench" /> <meta property="article:publisher" content="https://www.facebook.com/thermofisher" /> <meta property="article:published_time" content="2017-03-17T20:40:13+00:00" /> <meta property="article:modified_time" content="2017-03-20T19:20:09+00:00" /> <meta property="og:image" content="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/blog20hero201600x35020_1_-1.png" /> <meta property="og:image:width" content="1600" /> <meta property="og:image:height" content="350" /> <meta property="og:image:type" content="image/png" /> <meta name="author" content="Behind The Bench Staff" /> <meta name="twitter:card" content="summary_large_image" /> <meta name="twitter:creator" content="@thermofisher" /> <meta name="twitter:site" content="@thermofisher" /> <meta name="twitter:label1" content="Written by" /> <meta name="twitter:data1" content="Behind The Bench Staff" /> <meta name="twitter:label2" content="Est. reading time" /> <meta name="twitter:data2" content="5 minutes" /> <script type="application/ld+json" class="yoast-schema-graph">{"@context":"https://schema.org","@graph":[{"@type":"WebPage","@id":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/","url":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/","name":"Engineer Your Protein Using Directed Evolution Methods - Behind the Bench","isPartOf":{"@id":"https://www.thermofisher.com/blog/behindthebench/#website"},"primaryImageOfPage":{"@id":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/#primaryimage"},"image":{"@id":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/#primaryimage"},"thumbnailUrl":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/blog20hero201600x35020_1_-1.png","datePublished":"2017-03-17T20:40:13+00:00","dateModified":"2017-03-20T19:20:09+00:00","author":{"@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/9bbb2d67616274d3d4a21a584f7f4a0c"},"breadcrumb":{"@id":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/#primaryimage","url":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/blog20hero201600x35020_1_-1.png","contentUrl":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/blog20hero201600x35020_1_-1.png","width":1600,"height":350},{"@type":"BreadcrumbList","@id":"https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.thermofisher.com/blog/behindthebench/"},{"@type":"ListItem","position":2,"name":"Engineer Your Protein Using Directed Evolution Methods"}]},{"@type":"WebSite","@id":"https://www.thermofisher.com/blog/behindthebench/#website","url":"https://www.thermofisher.com/blog/behindthebench/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.thermofisher.com/blog/behindthebench/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/9bbb2d67616274d3d4a21a584f7f4a0c","name":"Behind The Bench Staff","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/image/","url":"https://secure.gravatar.com/avatar/d6e5815e57520094d932400e2af233b9?s=96&d=mm&r=g","contentUrl":"https://secure.gravatar.com/avatar/d6e5815e57520094d932400e2af233b9?s=96&d=mm&r=g","caption":"Behind The Bench Staff"},"url":"https://www.thermofisher.com/blog/author/behind-the-bench-staff/"}]}</script>  <link rel='dns-prefetch' href='//www.thermofisher.com' /> <link rel='dns-prefetch' href='//use.fontawesome.com' /> <link rel="alternate" type="application/rss+xml" title="Behind the Bench » Feed" href="https://www.thermofisher.com/blog/behindthebench/feed/" /> <link rel="alternate" type="application/rss+xml" title="Behind the Bench » Comments Feed" href="https://www.thermofisher.com/blog/behindthebench/comments/feed/" /> <link rel="alternate" type="application/rss+xml" title="Behind the Bench » Engineer Your Protein Using Directed Evolution Methods Comments Feed" href="https://www.thermofisher.com/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/feed/" />  <script src="https://cdn.optimizely.com/js/8156647427.js"></script>  <script> (function(h,o,t,j,a,r){ h.hj=h.hj||function(){(h.hj.q=h.hj.q||[]).push(arguments)}; h._hjSettings={hjid:373708,hjsv:5}; a=o.getElementsByTagName('head')[0]; r=o.createElement('script');r.async=1; r.src=t+h._hjSettings.hjid+j+h._hjSettings.hjsv; a.appendChild(r); })(window,document,'//static.hotjar.com/c/hotjar-','.js?sv='); </script>  <script src="//www.googletagmanager.com/gtag/js?id=G-K4QTN4KM25" data-cfasync="false" data-wpfc-render="false" type="text/javascript" async></script> <script data-cfasync="false" data-wpfc-render="false" type="text/javascript"> var mi_version = '9.2.0'; var mi_track_user = true; var mi_no_track_reason = ''; var MonsterInsightsDefaultLocations = {"page_location":"https:\/\/admin.acceleratingscience.com\/behindthebench\/engineer-your-protein-using-directed-evolution-methods\/"}; if ( typeof MonsterInsightsPrivacyGuardFilter === 'function' ) { var MonsterInsightsLocations = (typeof MonsterInsightsExcludeQuery === 'object') ? MonsterInsightsPrivacyGuardFilter( MonsterInsightsExcludeQuery ) : MonsterInsightsPrivacyGuardFilter( MonsterInsightsDefaultLocations ); } else { var MonsterInsightsLocations = (typeof MonsterInsightsExcludeQuery === 'object') ? MonsterInsightsExcludeQuery : MonsterInsightsDefaultLocations; } var disableStrs = [ 'ga-disable-G-K4QTN4KM25', ]; /* Function to detect opted out users */ function __gtagTrackerIsOptedOut() { for (var index = 0; index < disableStrs.length; index++) { if (document.cookie.indexOf(disableStrs[index] + '=true') > -1) { return true; } } return false; } /* Disable tracking if the opt-out cookie exists. */ if (__gtagTrackerIsOptedOut()) { for (var index = 0; index < disableStrs.length; index++) { window[disableStrs[index]] = true; } } /* Opt-out function */ function __gtagTrackerOptout() { for (var index = 0; index < disableStrs.length; index++) { document.cookie = disableStrs[index] + '=true; expires=Thu, 31 Dec 2099 23:59:59 UTC; path=/'; window[disableStrs[index]] = true; } } if ('undefined' === typeof gaOptout) { function gaOptout() { __gtagTrackerOptout(); } } window.dataLayer = window.dataLayer || []; window.MonsterInsightsDualTracker = { helpers: {}, trackers: {}, }; if (mi_track_user) { function __gtagDataLayer() { dataLayer.push(arguments); } function __gtagTracker(type, name, parameters) { if (!parameters) { parameters = {}; } if (parameters.send_to) { __gtagDataLayer.apply(null, arguments); return; } if (type === 'event') { parameters.send_to = monsterinsights_frontend.v4_id; var hookName = name; if (typeof parameters['event_category'] !== 'undefined') { hookName = parameters['event_category'] + ':' + name; } if (typeof MonsterInsightsDualTracker.trackers[hookName] !== 'undefined') { MonsterInsightsDualTracker.trackers[hookName](parameters); } else { __gtagDataLayer('event', name, parameters); } } else { __gtagDataLayer.apply(null, arguments); } } __gtagTracker('js', new Date()); __gtagTracker('set', { 'developer_id.dZGIzZG': true, }); if ( MonsterInsightsLocations.page_location ) { __gtagTracker('set', MonsterInsightsLocations); } __gtagTracker('config', 'G-K4QTN4KM25', {"use_amp_client_id":"true","forceSSL":"true","author":"user-120","category":"lab-tips-and-tricks,product-news","published_at":"2017-03-17T20:40:13+00:00","tags":"untagged"} ); window.gtag = __gtagTracker; (function () { /* https://developers.google.com/analytics/devguides/collection/analyticsjs/ */ /* ga and __gaTracker compatibility shim. */ var noopfn = function () { return null; }; var newtracker = function () { return new Tracker(); }; var Tracker = function () { return null; }; var p = Tracker.prototype; p.get = noopfn; p.set = noopfn; p.send = function () { var args = Array.prototype.slice.call(arguments); args.unshift('send'); __gaTracker.apply(null, args); }; var __gaTracker = function () { var len = arguments.length; if (len === 0) { return; } var f = arguments[len - 1]; if (typeof f !== 'object' || f === null || typeof f.hitCallback !== 'function') { if ('send' === arguments[0]) { var hitConverted, hitObject = false, action; if ('event' === arguments[1]) { if ('undefined' !== typeof arguments[3]) { hitObject = { 'eventAction': arguments[3], 'eventCategory': arguments[2], 'eventLabel': arguments[4], 'value': arguments[5] ? arguments[5] : 1, } } } if ('pageview' === arguments[1]) { if ('undefined' !== typeof arguments[2]) { hitObject = { 'eventAction': 'page_view', 'page_path': arguments[2], } } } if (typeof arguments[2] === 'object') { hitObject = arguments[2]; } if (typeof arguments[5] === 'object') { Object.assign(hitObject, arguments[5]); } if ('undefined' !== typeof arguments[1].hitType) { hitObject = arguments[1]; if ('pageview' === hitObject.hitType) { hitObject.eventAction = 'page_view'; } } if (hitObject) { action = 'timing' === arguments[1].hitType ? 'timing_complete' : hitObject.eventAction; hitConverted = mapArgs(hitObject); __gtagTracker('event', action, hitConverted); } } return; } function mapArgs(args) { var arg, hit = {}; var gaMap = { 'eventCategory': 'event_category', 'eventAction': 'event_action', 'eventLabel': 'event_label', 'eventValue': 'event_value', 'nonInteraction': 'non_interaction', 'timingCategory': 'event_category', 'timingVar': 'name', 'timingValue': 'value', 'timingLabel': 'event_label', 'page': 'page_path', 'location': 'page_location', 'title': 'page_title', 'referrer' : 'page_referrer', }; for (arg in args) { if (!(!args.hasOwnProperty(arg) || !gaMap.hasOwnProperty(arg))) { hit[gaMap[arg]] = args[arg]; } else { hit[arg] = args[arg]; } } return hit; } try { f.hitCallback(); } catch (ex) { } }; __gaTracker.create = newtracker; __gaTracker.getByName = newtracker; __gaTracker.getAll = function () { return []; }; __gaTracker.remove = noopfn; __gaTracker.loaded = true; window['__gaTracker'] = __gaTracker; })(); } else { console.log(""); (function () { function __gtagTracker() { return null; } window['__gtagTracker'] = __gtagTracker; window['gtag'] = __gtagTracker; })(); } </script>  <link rel='stylesheet' id='jetpack_related-posts-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/jetpack/modules/related-posts/related-posts.css?ver=20240116' type='text/css' media='all' /> <link rel='stylesheet' id='accelerating-science-blogs-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/themes/accelerating-science-blogs/style.css?ver=1.2.9.3' type='text/css' media='all' /> <link rel='stylesheet' id='wp-block-library-css' href='https://www.thermofisher.com/blog//wp-includes/css/dist/block-library/style.min.css?ver=6.6.1' type='text/css' media='all' /> <link rel='stylesheet' id='mediaelement-css' href='https://www.thermofisher.com/blog//wp-includes/js/mediaelement/mediaelementplayer-legacy.min.css?ver=4.2.17' type='text/css' media='all' /> <link rel='stylesheet' id='wp-mediaelement-css' href='https://www.thermofisher.com/blog//wp-includes/js/mediaelement/wp-mediaelement.min.css?ver=6.6.1' type='text/css' media='all' /> <style id='jetpack-sharing-buttons-style-inline-css' type='text/css'> .jetpack-sharing-buttons__services-list{display:flex;flex-direction:row;flex-wrap:wrap;gap:0;list-style-type:none;margin:5px;padding:0}.jetpack-sharing-buttons__services-list.has-small-icon-size{font-size:12px}.jetpack-sharing-buttons__services-list.has-normal-icon-size{font-size:16px}.jetpack-sharing-buttons__services-list.has-large-icon-size{font-size:24px}.jetpack-sharing-buttons__services-list.has-huge-icon-size{font-size:36px}@media print{.jetpack-sharing-buttons__services-list{display:none!important}}.editor-styles-wrapper .wp-block-jetpack-sharing-buttons{gap:0;padding-inline-start:0}ul.jetpack-sharing-buttons__services-list.has-background{padding:1.25em 2.375em} </style> <style id='classic-theme-styles-inline-css' type='text/css'> /*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} </style> <style id='global-styles-inline-css' type='text/css'> :root{--wp--preset--aspect-ratio--square: 1;--wp--preset--aspect-ratio--4-3: 4/3;--wp--preset--aspect-ratio--3-4: 3/4;--wp--preset--aspect-ratio--3-2: 3/2;--wp--preset--aspect-ratio--2-3: 2/3;--wp--preset--aspect-ratio--16-9: 16/9;--wp--preset--aspect-ratio--9-16: 9/16;--wp--preset--color--black: #000000;--wp--preset--color--cyan-bluish-gray: #abb8c3;--wp--preset--color--white: #ffffff;--wp--preset--color--pale-pink: #f78da7;--wp--preset--color--vivid-red: #cf2e2e;--wp--preset--color--luminous-vivid-orange: #ff6900;--wp--preset--color--luminous-vivid-amber: #fcb900;--wp--preset--color--light-green-cyan: #7bdcb5;--wp--preset--color--vivid-green-cyan: #00d084;--wp--preset--color--pale-cyan-blue: #8ed1fc;--wp--preset--color--vivid-cyan-blue: #0693e3;--wp--preset--color--vivid-purple: #9b51e0;--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple: linear-gradient(135deg,rgba(6,147,227,1) 0%,rgb(155,81,224) 100%);--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan: linear-gradient(135deg,rgb(122,220,180) 0%,rgb(0,208,130) 100%);--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange: linear-gradient(135deg,rgba(252,185,0,1) 0%,rgba(255,105,0,1) 100%);--wp--preset--gradient--luminous-vivid-orange-to-vivid-red: linear-gradient(135deg,rgba(255,105,0,1) 0%,rgb(207,46,46) 100%);--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray: linear-gradient(135deg,rgb(238,238,238) 0%,rgb(169,184,195) 100%);--wp--preset--gradient--cool-to-warm-spectrum: linear-gradient(135deg,rgb(74,234,220) 0%,rgb(151,120,209) 20%,rgb(207,42,186) 40%,rgb(238,44,130) 60%,rgb(251,105,98) 80%,rgb(254,248,76) 100%);--wp--preset--gradient--blush-light-purple: linear-gradient(135deg,rgb(255,206,236) 0%,rgb(152,150,240) 100%);--wp--preset--gradient--blush-bordeaux: linear-gradient(135deg,rgb(254,205,165) 0%,rgb(254,45,45) 50%,rgb(107,0,62) 100%);--wp--preset--gradient--luminous-dusk: linear-gradient(135deg,rgb(255,203,112) 0%,rgb(199,81,192) 50%,rgb(65,88,208) 100%);--wp--preset--gradient--pale-ocean: linear-gradient(135deg,rgb(255,245,203) 0%,rgb(182,227,212) 50%,rgb(51,167,181) 100%);--wp--preset--gradient--electric-grass: linear-gradient(135deg,rgb(202,248,128) 0%,rgb(113,206,126) 100%);--wp--preset--gradient--midnight: linear-gradient(135deg,rgb(2,3,129) 0%,rgb(40,116,252) 100%);--wp--preset--font-size--small: 13px;--wp--preset--font-size--medium: 20px;--wp--preset--font-size--large: 36px;--wp--preset--font-size--x-large: 42px;--wp--preset--spacing--20: 0.44rem;--wp--preset--spacing--30: 0.67rem;--wp--preset--spacing--40: 1rem;--wp--preset--spacing--50: 1.5rem;--wp--preset--spacing--60: 2.25rem;--wp--preset--spacing--70: 3.38rem;--wp--preset--spacing--80: 5.06rem;--wp--preset--shadow--natural: 6px 6px 9px rgba(0, 0, 0, 0.2);--wp--preset--shadow--deep: 12px 12px 50px rgba(0, 0, 0, 0.4);--wp--preset--shadow--sharp: 6px 6px 0px rgba(0, 0, 0, 0.2);--wp--preset--shadow--outlined: 6px 6px 0px -3px rgba(255, 255, 255, 1), 6px 6px rgba(0, 0, 0, 1);--wp--preset--shadow--crisp: 6px 6px 0px rgba(0, 0, 0, 1);}:where(.is-layout-flex){gap: 0.5em;}:where(.is-layout-grid){gap: 0.5em;}body .is-layout-flex{display: flex;}.is-layout-flex{flex-wrap: wrap;align-items: center;}.is-layout-flex > :is(*, div){margin: 0;}body .is-layout-grid{display: grid;}.is-layout-grid > :is(*, div){margin: 0;}:where(.wp-block-columns.is-layout-flex){gap: 2em;}:where(.wp-block-columns.is-layout-grid){gap: 2em;}:where(.wp-block-post-template.is-layout-flex){gap: 1.25em;}:where(.wp-block-post-template.is-layout-grid){gap: 1.25em;}.has-black-color{color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-color{color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-color{color: var(--wp--preset--color--white) !important;}.has-pale-pink-color{color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-color{color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-color{color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-color{color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-color{color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-color{color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-color{color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-color{color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-color{color: var(--wp--preset--color--vivid-purple) !important;}.has-black-background-color{background-color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-background-color{background-color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-background-color{background-color: var(--wp--preset--color--white) !important;}.has-pale-pink-background-color{background-color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-background-color{background-color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-background-color{background-color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-background-color{background-color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-background-color{background-color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-background-color{background-color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-background-color{background-color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-background-color{background-color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-background-color{background-color: var(--wp--preset--color--vivid-purple) !important;}.has-black-border-color{border-color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-border-color{border-color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-border-color{border-color: var(--wp--preset--color--white) !important;}.has-pale-pink-border-color{border-color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-border-color{border-color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-border-color{border-color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-border-color{border-color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-border-color{border-color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-border-color{border-color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-border-color{border-color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-border-color{border-color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-border-color{border-color: var(--wp--preset--color--vivid-purple) !important;}.has-vivid-cyan-blue-to-vivid-purple-gradient-background{background: var(--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple) !important;}.has-light-green-cyan-to-vivid-green-cyan-gradient-background{background: var(--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan) !important;}.has-luminous-vivid-amber-to-luminous-vivid-orange-gradient-background{background: var(--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange) !important;}.has-luminous-vivid-orange-to-vivid-red-gradient-background{background: var(--wp--preset--gradient--luminous-vivid-orange-to-vivid-red) !important;}.has-very-light-gray-to-cyan-bluish-gray-gradient-background{background: var(--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray) !important;}.has-cool-to-warm-spectrum-gradient-background{background: var(--wp--preset--gradient--cool-to-warm-spectrum) !important;}.has-blush-light-purple-gradient-background{background: var(--wp--preset--gradient--blush-light-purple) !important;}.has-blush-bordeaux-gradient-background{background: var(--wp--preset--gradient--blush-bordeaux) !important;}.has-luminous-dusk-gradient-background{background: var(--wp--preset--gradient--luminous-dusk) !important;}.has-pale-ocean-gradient-background{background: var(--wp--preset--gradient--pale-ocean) !important;}.has-electric-grass-gradient-background{background: var(--wp--preset--gradient--electric-grass) !important;}.has-midnight-gradient-background{background: var(--wp--preset--gradient--midnight) !important;}.has-small-font-size{font-size: var(--wp--preset--font-size--small) !important;}.has-medium-font-size{font-size: var(--wp--preset--font-size--medium) !important;}.has-large-font-size{font-size: var(--wp--preset--font-size--large) !important;}.has-x-large-font-size{font-size: var(--wp--preset--font-size--x-large) !important;} :where(.wp-block-post-template.is-layout-flex){gap: 1.25em;}:where(.wp-block-post-template.is-layout-grid){gap: 1.25em;} :where(.wp-block-columns.is-layout-flex){gap: 2em;}:where(.wp-block-columns.is-layout-grid){gap: 2em;} :root :where(.wp-block-pullquote){font-size: 1.5em;line-height: 1.6;} </style> <link rel='stylesheet' id='mashsb-styles-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashsharer/assets/css/mashsb.min.css?ver=4.0.47' type='text/css' media='all' /> <style id='mashsb-styles-inline-css' type='text/css'> .mashsb-count {color:#555759;}[class^="mashicon-"] .text, [class*=" mashicon-"] .text{ text-indent: -9999px !important; line-height: 0px; display: block; } [class^="mashicon-"] .text:after, [class*=" mashicon-"] .text:after { content: "" !important; text-indent: 0; font-size:13px; display: block !important; } [class^="mashicon-"], [class*=" mashicon-"] { width:25%; text-align: center !important; } [class^="mashicon-"] .icon:before, [class*=" mashicon-"] .icon:before { float:none; margin-right: 0; } .mashsb-buttons a{ margin-right: 3px; margin-bottom:3px; min-width: 0; width: 41px; } .onoffswitch, .onoffswitch-inner:before, .onoffswitch-inner:after .onoffswitch2, .onoffswitch2-inner:before, .onoffswitch2-inner:after { margin-right: 0px; width: 41px; line-height: 41px; } </style> <link rel='stylesheet' id='mashnet-styles-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashshare-networks/assets/css/mashnet.min.css?ver=2.5.3' type='text/css' media='all' /> <link rel='stylesheet' id='mashsas-styles-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashshare-select-and-share/assets/css/mashsas.min.css?ver=1.0.5' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_reset_css-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/legacy/css/formreset.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_formsmain_css-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/legacy/css/formsmain.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_ready_class_css-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/legacy/css/readyclass.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_browsers_css-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/legacy/css/browsers.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='globals-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/themes/accelerating-science-blogs/global-style.css?ver=1.2.9.3' type='text/css' media='all' /> <link rel='stylesheet' id='bfa-font-awesome-css' href='https://use.fontawesome.com/releases/v5.15.4/css/all.css?ver=2.0.3' type='text/css' media='all' /> <link rel='stylesheet' id='bfa-font-awesome-v4-shim-css' href='https://use.fontawesome.com/releases/v5.15.4/css/v4-shims.css?ver=2.0.3' type='text/css' media='all' /> <style id='bfa-font-awesome-v4-shim-inline-css' type='text/css'> @font-face { font-family: 'FontAwesome'; src: url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.eot'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.eot?#iefix') format('embedded-opentype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.woff2') format('woff2'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.woff') format('woff'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.ttf') format('truetype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.svg#fontawesome') format('svg'); } @font-face { font-family: 'FontAwesome'; src: url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.eot'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.eot?#iefix') format('embedded-opentype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.woff2') format('woff2'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.woff') format('woff'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.ttf') format('truetype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.svg#fontawesome') format('svg'); } @font-face { font-family: 'FontAwesome'; src: url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.eot'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.eot?#iefix') format('embedded-opentype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.woff2') format('woff2'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.woff') format('woff'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.ttf') format('truetype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.svg#fontawesome') format('svg'); unicode-range: U+F004-F005,U+F007,U+F017,U+F022,U+F024,U+F02E,U+F03E,U+F044,U+F057-F059,U+F06E,U+F070,U+F075,U+F07B-F07C,U+F080,U+F086,U+F089,U+F094,U+F09D,U+F0A0,U+F0A4-F0A7,U+F0C5,U+F0C7-F0C8,U+F0E0,U+F0EB,U+F0F3,U+F0F8,U+F0FE,U+F111,U+F118-F11A,U+F11C,U+F133,U+F144,U+F146,U+F14A,U+F14D-F14E,U+F150-F152,U+F15B-F15C,U+F164-F165,U+F185-F186,U+F191-F192,U+F1AD,U+F1C1-F1C9,U+F1CD,U+F1D8,U+F1E3,U+F1EA,U+F1F6,U+F1F9,U+F20A,U+F247-F249,U+F24D,U+F254-F25B,U+F25D,U+F267,U+F271-F274,U+F279,U+F28B,U+F28D,U+F2B5-F2B6,U+F2B9,U+F2BB,U+F2BD,U+F2C1-F2C2,U+F2D0,U+F2D2,U+F2DC,U+F2ED,U+F328,U+F358-F35B,U+F3A5,U+F3D1,U+F410,U+F4AD; } </style> <link rel='stylesheet' id='boxzilla-css' href='https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/boxzilla/assets/css/styles.css?ver=3.3.1' type='text/css' media='all' /> <script type="text/javascript" id="jetpack_related-posts-js-extra"> /* <![CDATA[ */ var related_posts_js_options = {"post_heading":"h4"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/jetpack/_inc/build/related-posts/related-posts.min.js?ver=20240116" id="jetpack_related-posts-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/google-analytics-premium/assets/js/frontend-gtag.min.js?ver=9.2.0" id="monsterinsights-frontend-script-js" async="async" data-wp-strategy="async"></script> <script data-cfasync="false" data-wpfc-render="false" type="text/javascript" id='monsterinsights-frontend-script-js-extra'>/* <![CDATA[ */ var monsterinsights_frontend = {"js_events_tracking":"true","download_extensions":"doc,pdf,ppt,zip,xls,docx,pptx,xlsx","inbound_paths":"[]","home_url":"https:\/\/admin.acceleratingscience.com\/behindthebench","hash_tracking":"false","v4_id":"G-K4QTN4KM25"};/* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/jquery.min.js?ver=3.7.1" id="jquery-core-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/jquery-migrate.min.js?ver=3.4.1" id="jquery-migrate-js"></script> <script type="text/javascript" id="mashsb-js-extra"> /* <![CDATA[ */ var mashsb = {"shares":"2","round_shares":"1","animate_shares":"0","dynamic_buttons":"0","share_url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/engineer-your-protein-using-directed-evolution-methods\/","title":"Engineer+Your+Protein+Using+Directed+Evolution+Methods","image":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/03\/blog20hero201600x35020_1_-1.png","desc":"Would you like to evolve your protein of interest but are tired of error-prone PCR approaches? Libraries based on synthetic templates have many advantages and could be the solution for you.\nSynthetic biology has now paved \u2026","hashtag":"thermofisher","subscribe":"content","subscribe_url":"","activestatus":"1","singular":"1","twitter_popup":"1","refresh":"1","nonce":"e79464e1f0","postid":"10283","servertime":"1732697985","ajaxurl":"https:\/\/www.thermofisher.com\/blog\/wp-admin\/admin-ajax.php"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashsharer/assets/js/mashsb.min.js?ver=4.0.47" id="mashsb-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashshare-google-analytics/assets/js/mashga.min.js?ver=1.0.9" id="mashga-js"></script> <script type="text/javascript" id="mashnet-js-extra"> /* <![CDATA[ */ var mashnet = {"body":"Check out this article I just read:","subject":"Check out this article from Accelerating Science","pinterest_select":"1","pinterest_image":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/03\/blog20hero201600x35020_1_-1.png","pinterest_desc":"Would you like to evolve your protein of interest but are tired of error-prone PCR approaches? Libraries based on synthetic templates have many advantages and could be the solution for you.\nSynthetic biology has now paved \u2026"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashshare-networks/assets/js/mashnet.min.js?ver=2.5.3" id="mashnet-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/underscore.min.js?ver=1.13.4" id="underscore-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/backbone.min.js?ver=1.5.0" id="backbone-js"></script> <script type="text/javascript" id="wp-api-js-js-extra"> /* <![CDATA[ */ var WP_API_Settings = {"root":"https:\/\/admin.acceleratingscience.com\/behindthebench","nonce":"29e0fbf17e"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/wp-search-live/public/assets/js/util--wp-api.js?ver=0.9" id="wp-api-js-js"></script> <script type="text/javascript" id="mashsas-js-extra"> /* <![CDATA[ */ var mashsas = {"minchars":"10","appid":"1603510369972799","facebook":"1","twitter":"1","mail":"1"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/mashshare-select-and-share/assets/js/mashsas.min.js?ver=1.0.5" id="mashsas-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/js/jquery.json.min.js?ver=2.8.17" id="gform_json-js"></script> <script type="text/javascript" id="gform_gravityforms-js-extra"> /* <![CDATA[ */ var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gform_i18n = {"datepicker":{"days":{"monday":"Mo","tuesday":"Tu","wednesday":"We","thursday":"Th","friday":"Fr","saturday":"Sa","sunday":"Su"},"months":{"january":"January","february":"February","march":"March","april":"April","may":"May","june":"June","july":"July","august":"August","september":"September","october":"October","november":"November","december":"December"},"firstDay":1,"iconText":"Select date"}}; var gf_legacy_multi = {"1":"1","":"1"}; var gform_gravityforms = {"strings":{"invalid_file_extension":"This type of file is not allowed. Must be one of the following:","delete_file":"Delete this file","in_progress":"in progress","file_exceeds_limit":"File exceeds size limit","illegal_extension":"This type of file is not allowed.","max_reached":"Maximum number of files reached","unknown_error":"There was a problem while saving the file on the server","currently_uploading":"Please wait for the uploading to complete","cancel":"Cancel","cancel_upload":"Cancel this upload","cancelled":"Cancelled"},"vars":{"images_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms\/images"}}; /* ]]> */ </script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/js/gravityforms.min.js?ver=2.8.17" id="gform_gravityforms-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/js/chosen.jquery.min.js?ver=2.8.17" id="gform_chosen-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/assets/js/dist/utils.min.js?ver=50c7bea9c2320e16728e44ae9fde5f26" id="gform_gravityforms_utils-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/themes/accelerating-science-blogs/js/single-scripts.js?ver=1.2.9.3" id="single-scripts-js"></script> <link rel="https://api.w.org/" href="https://www.thermofisher.com/blog/behindthebench/wp-json/" /><link rel="alternate" title="JSON" type="application/json" href="https://www.thermofisher.com/blog/behindthebench/wp-json/wp/v2/posts/10283" /><link rel="EditURI" type="application/rsd+xml" title="RSD" href="https://www.thermofisher.com/blog/xmlrpc.php?rsd" /> <link rel='shortlink' href='https://www.thermofisher.com/blog/behindthebench/?p=10283' /> <link rel="alternate" title="oEmbed (JSON)" type="application/json+oembed" href="https://www.thermofisher.com/blog/behindthebench/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fengineer-your-protein-using-directed-evolution-methods%2F" /> <link rel="alternate" title="oEmbed (XML)" type="text/xml+oembed" href="https://www.thermofisher.com/blog/behindthebench/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fengineer-your-protein-using-directed-evolution-methods%2F&format=xml" /> <meta property="fb:pages" content="258357757657262" />  <link rel="icon" href="https://www.thermofisher.com/blog/behindthebench/wp-content/themes/accelerating-science-blogs/images/favicon.ico" />  <script async src="https://www.googletagmanager.com/gtag/js?id=UA-34877942-1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'UA-34877942-1'); </script>  <script> !function(f,b,e,v,n,t,s){if(f.fbq)return;n=f.fbq=function(){n.callMethod? n.callMethod.apply(n,arguments):n.queue.push(arguments)};if(!f._fbq)f._fbq=n; n.push=n;n.loaded=!0;n.version='2.0';n.queue=[];t=b.createElement(e);t.async=!0; t.src=v;s=b.getElementsByTagName(e)[0];s.parentNode.insertBefore(t,s)}(window, document,'script','https://connect.facebook.net/en_US/fbevents.js'); fbq('init', '305572349637096'); fbq('track', "PageView");</script> <noscript><img height="1" width="1" style="display:none" src="https://www.facebook.com/tr?id=305572349637096&ev=PageView&noscript=1" /></noscript>   <script async src="https://www.googletagmanager.com/gtag/js?id=AW-1013120100"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'AW-1013120100'); </script>  <script> gtag('event', 'conversion', {'send_to': 'AW-1013120100/_RFLCLT6yqsBEOT4i-MD'}); </script> <script type="application/ld+json"> {"@context":"http:\/\/schema.org","@type":"BlogPosting","mainEntityofPage":{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/engineer-your-protein-using-directed-evolution-methods\/"},"headline":"Engineer Your Protein Using Directed Evolution Methods","datePublished":"03\/17\/2017","dateModified":"03\/17\/2017","publisher":{"@type":"Organization","name":"Thermo Fisher Scientific","URL":"https:\/\/www.thermofisher.com\/us\/en\/home.html","logo":{"@type":"ImageObject","url":"https:\/\/www.thermofisher.com\/content\/dam\/LifeTech\/Images\/Header\/logo-color.png"}},"image":{"@type":"ImageObject","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/03\/blog20hero201600x35020_1_-1.png","width":1600,"height":350},"author":{"@type":"Person","name":"Behind The Bench Staff"}} </script> <script type="text/javascript" src="https://www.thermofisher.com/etc/designs/lifetech/clientlibs/digitaldata.js" defer="defer"></script> <script id="tagmanager" type="text/javascript" src='https://assets.adobedtm.com/launch-ENeecb77f1160a4928a492ff040f7110c7.min.js' defer="defer"></script> <script> (function($) { $(document).ready(function() { digitalData.setPageType('blog'); }) })(jQuery); </script> <style type="text/css" id="wp-custom-css"> /* Welcome to Custom CSS! To learn how this works, see http://wp.me/PEmnE-Bt */blockquote { width: 450px; color:#FFF; background: #7d7e7d; /* Old browsers */ background: -moz-linear-gradient(top, #7d7e7d 0%, #0e0e0e 100%); /* FF3.6+ */ background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,#7d7e7d), color-stop(100%,#0e0e0e)); /* Chrome,Safari4+ */ background: -webkit-linear-gradient(top, #7d7e7d 0%,#0e0e0e 100%); /* Chrome10+,Safari5.1+ */ background: -o-linear-gradient(top, #7d7e7d 0%,#0e0e0e 100%); /* Opera 11.10+ */ background: -ms-linear-gradient(top, #7d7e7d 0%,#0e0e0e 100%); /* IE10+ */ background: linear-gradient(to bottom, #7d7e7d 0%,#0e0e0e 100%); /* W3C */ filter: progid:DXImageTransform.Microsoft.gradient( startColorstr=’#7d7e7d’, endColorstr=’#0e0e0e’,GradientType=0 ); /* IE6-9 */ border: 1px solid #ccc; border-radius: 6px; box-shadow: 1px 1px 1px #ccc; font-style: italic; } </style> </head> <body class="post-template-default single single-post postid-10283 single-format-standard header-image full-width-content genesis-breadcrumbs-visible"><div class="site-container"><header class="site-header"><div class="wrap"><div class="title-area"><p class="site-title"><a href="https://www.thermofisher.com/" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></p></div><div class="widget-area header-widget-area"><section id="nav_menu-2" class="widget widget_nav_menu"><div class="widget-wrap"><nav class="nav-header"><ul id="menu-main-menu" class="menu genesis-nav-menu"><li id="menu-item-15" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-15"><a><span >Categories</span></a> <ul class="sub-menu"> <li id="menu-item-22" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-22"><a href="https://www.thermofisher.com/blog/materials/"><span >Advancing Materials</span></a></li> <li id="menu-item-21" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-21"><a href="https://www.thermofisher.com/blog/mining/"><span >Advancing Mining</span></a></li> <li id="menu-item-17937" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-17937"><a href="https://www.thermofisher.com/blog/analyteguru/"><span >AnalyteGuru</span></a></li> <li id="menu-item-20" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-20"><a href="https://www.thermofisher.com/blog/metals/"><span >Analyzing Metals</span></a></li> <li id="menu-item-12913" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-12913"><a href="https://www.thermofisher.com/blog/ask-a-scientist/"><span >Ask a Scientist</span></a></li> <li id="menu-item-16" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-16"><a href="https://www.thermofisher.com/blog/behindthebench/"><span >Behind the Bench</span></a></li> <li id="menu-item-17769" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-17769"><a href="https://www.thermofisher.com/blog/biotechnology/"><span >Biotech at Scale</span></a></li> <li id="menu-item-16657" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-16657"><a href="https://www.thermofisher.com/blog/clinical-conversations/"><span >Clinical Conversations</span></a></li> <li id="menu-item-19" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-19"><a href="https://www.thermofisher.com/blog/food/"><span >Examining Food</span></a></li> <li id="menu-item-10924" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-10924"><a href="https://www.thermofisher.com/blog/identifying-threats/"><span >Identifying Threats</span></a></li> <li id="menu-item-16704" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-16704"><a href="https://www.thermofisher.com/blog/semiconductors/"><span >Illuminating Semiconductors</span></a></li> <li id="menu-item-16705" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-16705"><a href="https://www.thermofisher.com/blog/atomic-resolution/"><span >Life in Atomic Resolution</span></a></li> <li id="menu-item-10922" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-10922"><a href="https://www.thermofisher.com/blog/life-in-the-lab/"><span >Life in the Lab</span></a></li> <li id="menu-item-16658" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-16658"><a href="https://www.thermofisher.com/blog/oempowered/"><span >OEMpowered</span></a></li> <li id="menu-item-12812" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-12812"><a href="https://www.thermofisher.com/blog/connectedlab/"><span >The Connected Lab</span></a></li> </ul> </li> <li id="menu-item-24" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-24"><a href="https://www.thermofisher.com/blog/about-us/"><span >About Us</span></a></li> <li id="menu-item-25" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-25"><a href="https://www.thermofisher.com/blog/contact-us/?site=behindthebench"><span >Contact</span></a></li> </ul></nav></div></section> </div><div class="breadcrumb"><span class="breadcrumb-link-wrap"><a href="https://www.thermofisher.com/blog/?">Accelerating Science</a></span><span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/behindthebench/"><span class="breadcrumb-link-text-wrap">Behind the Bench</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/behindthebench/lab-tips-and-tricks/"><span class="breadcrumb-link-text-wrap">Lab Tips and Tricks</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">Engineer Your Protein Using Directed Evolution Methods</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-10283 post type-post status-publish format-standard has-post-thumbnail category-lab-tips-and-tricks category-product-news entry" aria-label="Engineer Your Protein Using Directed Evolution Methods"><header class="entry-header"><h1 class="entry-title">Engineer Your Protein Using Directed Evolution Methods</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/behind-the-bench-staff/" title="Posts by Behind The Bench Staff" rel="author">Behind The Bench Staff</a> </span> <span class="post-info-date"> 03.17.2017 </span> </header><div class="entry-content"><p><img decoding="async" src="https://www.thermofisher.com/blog/wp-content/uploads/sites/9/2017/03/blog20hero201600x35020_1_-1.png" /></p> <p><span>Would you like to </span><a href="https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/directed-evolution.html?cid=bid_SB_GNS_DirectedEvol_blog_20170316de" target="_blank"><span>evolve your protein of interest</span></a><span> but are tired of error-prone PCR approaches? Libraries based on synthetic templates have many advantages and could be the solution for you.</span></p> <p><a href="http://www.thermofisher.com/us/en/home/life-science/cloning/synthetic-biology.html?cid=bid_SB_GNS_DirectedEvol_blog_20170316synbio" target="_blank"><span>Synthetic biology</span></a><span> has now paved the way to create genes and libraries that require no natural template at all—almost any design vision is possible. Synthetic genes are well suited for optimizing protein expression in a non-natural host or to acquire genes that are hard to find in natural sources. </span><a href="https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis.html?cid=bid_SB_GNS_DirectedEvol_blog_20170316gns" target="_blank"><span>DNA synthesis</span></a><span> is based on the orderly assembly of short, chemically produced oligonucleotides. The same methodology can also be used to synthesize a library that mimics the error-prone PCR. During automated oligonucleotide synthesis, a definable frequency of misincorporation of an alternative residue is allowed for certain positions by adding distinct impurities to the corresponding reagent bottle. Based on these ‘spiked’ oligonucleotides, a controlled randomization library can be assembled:</span></p> <ul> <li><span><span>        </span></span><span>With the statistically expected ratio of transitions and transversions</span></li> <li><span><span>        </span></span><span>Where the average number of substitutions can be fine-tuned very accurately</span></li> <li><span><span>        </span></span><span>Where the positions of possible substitutions are not entirely random anymore, but limited to regions of interest defined solely by the investigator</span></li> </ul> <p><strong><span>How many mutations should one variant carry on average?</span></strong></p> <p><span>In general, a good rule of thumb is to limit the complexity of the library to still allow for the occurrence of the wild type gene in the screened subset. This helps ensure that the examined variants don’t diverge too much from the original protein, which could result in a loss of function for the complete pool.</span></p> <p><strong><span>Definition of regions of interest for which mutations should be introduced?</span></strong></p> <p><span>This question can be approached in many different ways, depending on the individual project. If the three dimensional structure of the investigated protein (or a highly similar one) is available, the amino acid residues likely related to the protein’s function (for example the active site of an enzyme) can sometimes be deduced and confirmed by single point mutations. If this data is available, it is a good idea to limit randomness to only these positions and to those that are in close three-dimensional proximity in a degenerated library. The remaining scaffold of the protein may be left unmodified.</span></p> <p><span>Another approach uses the availability of natural homologous sequences in protein or DNA databases. These can be aligned according to their similarity to identify regions of conservation and variation, reflecting the natural selection pressure for the particular gene family. Depending on the overall similarity within the family and the scope of the aimed protein adaptation, a library design will provide for new mutations either in the conserved or the variable part of the gene. One example is antibodies which have a very conserved framework, but are highly variable within the antigen-binding pockets and are best randomized in these regions to identify new binding specificities.</span></p> <p><span>If no such data is available, or if it does not correlate with the protein performance, the protein function topology can be mapped ahead of designing evolution experiments. A common tool is an alanine scan, which comprises the generation of all consecutive variants with each single amino acid being changed to alanine. The testing of these variants for maintenance or loss of function is an indicator of the importance of each protein position and usually identifies clusters of interest for further mutagenesis. The extreme application of this strategy is to change each individual amino acid not only to alanine but to all 19 non–wild type residues. This ‘sequential permutation’ of a 300 amino acid protein, involves the screening of 300 x 19 = 5,700 variants, which is manageable, even with low-throughput assays.</span></p> <p><span><div style="width: 809px" class="wp-caption alignmiddle"><img decoding="async" src="https://www.thermofisher.com/blog/wp-content/uploads/sites/9/2017/03/directed20evolution20workflow-1.jpg" style="vertical-align: middle" width="799" height="131" /><p class="wp-caption-text">Figure 1. Example workflow for streamlined identification of beneficial mutations within your target protein</p></div></span></p> <p><span>The functional analysis of these mutants generates a data matrix containing information about the importance of each amino acid position regarding overall function, and which non–wild type amino acid contributes to the adaptation of the protein towards the technical demands. In this type of experiment it is also common to identify beneficial mutations at amino acid positions that were not previously considered to be related to protein performance. With this valuable information at hand, it is possible to combine the advancing single mutations in a new combinatorial library to screen for synergistic beneficial effects. These second-round libraries typically concentrate on not more than 10 sites with either the wild type or the improving amino acid present. Thus, the size of this library is only ~210 = 1024, and can be fully screened even in complicated assays. As the demand for adapted proteins for industrial and medical applications increases, so will the scope of approaches to tailor them.</span></p> <p><span>Get more information and case studies on our </span><a href="https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/directed-evolution.html?cid=bid_SB_GNS_DirectedEvol_blog_20170316de" target="_blank"><span>Invitrogen™ GeneArt™ Directed Evolution services website >></span></a></p> <p><span>Learn more about our Directed Evolution methods for protein engineering in our recorded webinar. Time-, effort-, and cost- effective alternatives for your protein research will be discussed. This webinar includes an exemplary case study comparing traditional error-prone PCR method versus a rationally designed library.</span></p> <p><a href="https://find.thermofisher.com/synbio/geneartdnalibrarieswebinar/fb/webinar-registration-38225X-589043.html?cid=bid_SB_GNS_DirectedEvol_blog_20170316wbnr" target="_blank"><span>Watch our webinar: Directed evolution methods for protein engineering >></span></a></p> <p><em><span> </span></em></p> <p><em><span>For Research Use Only. Not for use in diagnostic procedures.</span></em></p> <p><span>Sources:</span></p> <p><span>https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/directed-evolution.html</span></p> <p> </p> <p> </p> <aside class="mashsb-container mashsb-main "><div class="mashsb_above_buttons">Share this article </div><div class="mashsb-box"><div class="mashsb-buttons"><a class="mashicon-facebook mash-large mashsb-noshadow" href="https://www.facebook.com/sharer.php?u=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fengineer-your-protein-using-directed-evolution-methods%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Facebook</span></a><a class="mashicon-linkedin mash-large mashsb-noshadow" href="https://www.linkedin.com/shareArticle?trk=Engineer+Your+Protein+Using+Directed+Evolution+Methods&url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fengineer-your-protein-using-directed-evolution-methods%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Linkedin</span></a><a class="mashicon-twitter mash-large mashsb-noshadow" href="https://twitter.com/intent/tweet?text=Engineer%20Your%20Protein%20Using%20Directed%20Evolution%20Methods&url=https://www.thermofisher.com/blog/behindthebench/?p=10283&via=thermofisher" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Twitter</span></a><a class="mashicon-mail mash-large mashsb-noshadow" href="mailto:?subject=Check%20out%20this%20article%20from%20Accelerating%20Science&body=Check%20out%20this%20article%20I%20just%20read%3Ahttps%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fengineer-your-protein-using-directed-evolution-methods%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Mail</span></a><div class="onoffswitch2 mash-large mashsb-noshadow" style="display:none"></div></div> </div> <div style="clear:both"></div><div class="mashsb-toggle-container"></div></aside> </div></article> <div id="respond" class="comment-respond"> <h3 id="reply-title" class="comment-reply-title">Leave a Reply <small><a rel="nofollow" id="cancel-comment-reply-link" href="/behindthebench/engineer-your-protein-using-directed-evolution-methods/#respond" style="display:none;">Cancel reply</a></small></h3><form action="https://www.thermofisher.com/blog/behindthebench/wp-comments-post.php" method="post" id="commentform" class="comment-form" novalidate><p class="comment-notes"><span id="email-notes">Your email address will not be published.</span> <span class="required-field-message">Required fields are marked <span class="required">*</span></span></p><p class="comment-form-comment"><label for="comment">Comment <span class="required">*</span></label> <textarea id="comment" name="comment" cols="45" rows="8" maxlength="65525" required></textarea></p><p class="comment-form-author"><label for="author">Name <span class="required">*</span></label> <input id="author" name="author" type="text" value="" size="30" maxlength="245" autocomplete="name" required /></p> <p class="comment-form-email"><label for="email">Email <span class="required">*</span></label> <input id="email" name="email" type="email" value="" size="30" maxlength="100" aria-describedby="email-notes" autocomplete="email" required /></p> <p class="comment-form-url"><label for="url">Website</label> <input id="url" name="url" type="url" value="" size="30" maxlength="200" autocomplete="url" /></p> <p class="form-submit"><input name="submit" type="submit" id="submit" class="submit" value="Post Comment" /> <input type='hidden' name='comment_post_ID' value='10283' id='comment_post_ID' /> <input type='hidden' name='comment_parent' id='comment_parent' value='0' /> </p><p style="display: none;"><input type="hidden" id="akismet_comment_nonce" name="akismet_comment_nonce" value="d0fee1feda" /></p><p style="display: none !important;" class="akismet-fields-container" data-prefix="ak_"><label>Δ<textarea name="ak_hp_textarea" cols="45" rows="8" maxlength="100"></textarea></label><input type="hidden" id="ak_js_2" name="ak_js" value="113"/><script>document.getElementById( "ak_js_2" ).setAttribute( "value", ( new Date() ).getTime() );</script></p></form> </div> <div class="adjacent-entry-pagination pagination"><div class="pagination-previous alignleft"><i class="fa fa-icon fa-chevron-left"></i> <a href="https://www.thermofisher.com/blog/behindthebench/can-ancestry-snps-generate-investigative-leads/" rel="prev">Can Ancestry SNPs Generate Investigative Leads?</a></div><div class="pagination-next alignright"><a href="https://www.thermofisher.com/blog/behindthebench/fortune-favors-the-prepared-mind-developing-the-zika-triplex-assay/" rel="next">Fortune Favors the Prepared Mind – Developing the Zika Triplex Assay</a> <i class="fa fa-icon fa-chevron-right"></i></div></div></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2024 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. All Rights Reserved.</p></div><span class="social-links"><p>Talk to us</p><a href="https://www.facebook.com/thermofisher" class="facebook" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-facebook"></i></a><a href="https://www.linkedin.com/company/thermo-fisher-scientific" class="linkedin" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-linkedin"></i></a><a href="https://twitter.com/thermofisher" class="twitter" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-twitter"></i></a><a href="https://www.youtube.com/c/thermofisher" class="youtube" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-youtube"></i></a></span></div></footer></div><div style="display: none;"><div id="boxzilla-box-10626-content"><p><h4>Get news and research reviews on the topic of your choice, right in your inbox.</h4> </p> <p><a id="subscribe" class="button" href="#">Subscribe Now</a></p> <div id="subscribe-form"> <div class='gf_browser_ie gf_browser_ie7 gform_wrapper gform_legacy_markup_wrapper gform-theme--no-framework' data-form-theme='legacy' data-form-index='1' id='gform_wrapper_1' ><form method='post' enctype='multipart/form-data' id='gform_1' action='/blog/behindthebench/engineer-your-protein-using-directed-evolution-methods/' formid='1'> <div class='gform-body gform_body'><ul id='gform_fields_1' class='gform_fields top_label form_sublabel_above description_below validation_below'><li id="field_1_4" class="gfield gfield--type-text first-name field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_4" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_4'>First Name</label><div class='ginput_container ginput_container_text'><input name='input_4' id='input_1_4' type='text' value='' class='large' placeholder='First Name' aria-invalid="false" /> </div></li><li id="field_1_5" class="gfield gfield--type-text last-name field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_5" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_5'>Last Name</label><div class='ginput_container ginput_container_text'><input name='input_5' id='input_1_5' type='text' value='' class='large' placeholder='Last Name' aria-invalid="false" /> </div></li><li id="field_1_2" class="gfield gfield--type-email email gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_2" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_2'>Email<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_email'> <input name='input_2' id='input_1_2' type='email' value='' class='large' placeholder='Email' aria-required="true" aria-invalid="false" /> </div></li><li id="field_1_6" class="gfield gfield--type-text company field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_6" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_6'>Company</label><div class='ginput_container ginput_container_text'><input name='input_6' id='input_1_6' type='text' value='' class='large' placeholder='Company' aria-invalid="false" /> </div></li><li id="field_1_7" class="gfield gfield--type-select country gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_7" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_7'>Select your country/region<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_select'><select name='input_7' id='input_1_7' class='large gfield_select' aria-required="true" aria-invalid="false" ><option value='' selected='selected' class='gf_placeholder'>Select your country/region</option><option value='United States' >United States</option><option value='Canada' >Canada</option><option value='Afghanistan' >Afghanistan</option><option value='Albania' >Albania</option><option value='Algeria' >Algeria</option><option value='American Samoa' >American Samoa</option><option value='Andorra' >Andorra</option><option value='Angola' >Angola</option><option value='Anguilla' >Anguilla</option><option value='Antarctica' >Antarctica</option><option value='Antigua and Barbuda' >Antigua and Barbuda</option><option value='Argentina' >Argentina</option><option value='Armenia' >Armenia</option><option value='Aruba' >Aruba</option><option value='Australia' >Australia</option><option value='Austria' >Austria</option><option value='Azerbaijan' >Azerbaijan</option><option value='Bahamas' >Bahamas</option><option value='Bahrain' >Bahrain</option><option value='Bangladesh' >Bangladesh</option><option value='Barbados' >Barbados</option><option value='Belarus' >Belarus</option><option value='Belgium' >Belgium</option><option value='Belize' >Belize</option><option value='Benin' >Benin</option><option value='Bermuda' >Bermuda</option><option value='Bhutan' >Bhutan</option><option value='Bolivia' >Bolivia</option><option value='Bosnia and Herzegovina' >Bosnia and Herzegovina</option><option value='Botswana' >Botswana</option><option value='Bouvet Island' >Bouvet Island</option><option value='Brazil' >Brazil</option><option value='British Indian Ocean Territory' >British Indian Ocean Territory</option><option value='Brunei Darussalam' >Brunei Darussalam</option><option value='Bulgaria' >Bulgaria</option><option value='Burkina Faso' >Burkina Faso</option><option value='Burundi' >Burundi</option><option value='Cambodia' >Cambodia</option><option value='Cameroon' >Cameroon</option><option value='Cape Verde' >Cape Verde</option><option value='Cayman Islands' >Cayman Islands</option><option value='Central African Republic' >Central African Republic</option><option value='Chad' >Chad</option><option value='Chile' >Chile</option><option value='China' >China</option><option value='Christmas Island' >Christmas Island</option><option value='Cocos (Keeling) Islands' >Cocos (Keeling) Islands</option><option value='Colombia' >Colombia</option><option value='Comoros' >Comoros</option><option value='Congo' >Congo</option><option value='Congo, The Democratic Republic of' >Congo, The Democratic Republic of</option><option value='Cook Islands' >Cook Islands</option><option value='Costa Rica' >Costa Rica</option><option value='Cote D'Ivoire' >Cote D'Ivoire</option><option value='Croatia' >Croatia</option><option value='Cuba' >Cuba</option><option value='Cyprus' >Cyprus</option><option value='Czech Republic' >Czech Republic</option><option value='Denmark' >Denmark</option><option value='Djibouti' >Djibouti</option><option value='Dominica' >Dominica</option><option value='Dominican Republic' >Dominican Republic</option><option value='East Timor' >East Timor</option><option value='Ecuador' >Ecuador</option><option value='Egypt' >Egypt</option><option value='El Salvador' >El Salvador</option><option value='Equatorial Guinea' >Equatorial Guinea</option><option value='Eritrea' >Eritrea</option><option value='Estonia' >Estonia</option><option value='Ethiopia' >Ethiopia</option><option value='Falkland Islands (Malvinas)' >Falkland Islands (Malvinas)</option><option value='Faroe Islands' >Faroe Islands</option><option value='Fiji' >Fiji</option><option value='Finland' >Finland</option><option value='Fmr Yugoslav Rep of Macedonia' >Fmr Yugoslav Rep of Macedonia</option><option value='France' >France</option><option value='French Guiana' >French Guiana</option><option value='French Polynesia' >French Polynesia</option><option value='French Southern Territories' >French Southern Territories</option><option value='Gabon' >Gabon</option><option value='Gambia' >Gambia</option><option value='Georgia' >Georgia</option><option value='Germany' >Germany</option><option value='Ghana' >Ghana</option><option value='Gibraltar' >Gibraltar</option><option value='Greece' >Greece</option><option value='Greenland' >Greenland</option><option value='Grenada' >Grenada</option><option value='Guadeloupe' >Guadeloupe</option><option value='Guam' >Guam</option><option value='Guatemala' >Guatemala</option><option value='Guinea' >Guinea</option><option value='Guinea-Bissau' >Guinea-Bissau</option><option value='Guyana' >Guyana</option><option value='Haiti' >Haiti</option><option value='Heard and McDonald Islands' >Heard and McDonald Islands</option><option value='Holy See (Vatican City State)' >Holy See (Vatican City State)</option><option value='Honduras' >Honduras</option><option value='Hong Kong' >Hong Kong</option><option value='Hungary' >Hungary</option><option value='Iceland' >Iceland</option><option value='India' >India</option><option value='Indonesia' >Indonesia</option><option value='Iran (Islamic Republic Of)' >Iran (Islamic Republic Of)</option><option value='Iraq' >Iraq</option><option value='Ireland' >Ireland</option><option value='Israel' >Israel</option><option value='Italy' >Italy</option><option value='Jamaica' >Jamaica</option><option value='Japan' >Japan</option><option value='Jordan' >Jordan</option><option value='Kazakstan' >Kazakstan</option><option value='Kenya' >Kenya</option><option value='Kiribati' >Kiribati</option><option value='Korea, Democratic People's Rep' >Korea, Democratic People's Rep</option><option value='Korea, Republic of' >Korea, Republic of</option><option value='Kuwait' >Kuwait</option><option value='Kyrgyzstan' >Kyrgyzstan</option><option value='Lao People's Democratic Rep' >Lao People's Democratic Rep</option><option value='Latvia' >Latvia</option><option value='Lebanon' >Lebanon</option><option value='Lesotho' >Lesotho</option><option value='Liberia' >Liberia</option><option value='Libyan Arab Jamahiriya' >Libyan Arab Jamahiriya</option><option value='Liechtenstein' >Liechtenstein</option><option value='Lithuania' >Lithuania</option><option value='Luxembourg' >Luxembourg</option><option value='Macau' >Macau</option><option value='Madagascar' >Madagascar</option><option value='Malawi' >Malawi</option><option value='Malaysia' >Malaysia</option><option value='Maldives' >Maldives</option><option value='Mali' >Mali</option><option value='Malta' >Malta</option><option value='Marshall Islands' >Marshall Islands</option><option value='Martinique' >Martinique</option><option value='Mauritania' >Mauritania</option><option value='Mauritius' >Mauritius</option><option value='Mayotte' >Mayotte</option><option value='Mexico' >Mexico</option><option value='Micronesia, Federated States' >Micronesia, Federated States</option><option value='Moldova, Republic of' >Moldova, Republic of</option><option value='Monaco' >Monaco</option><option value='Mongolia' >Mongolia</option><option value='Montserrat' >Montserrat</option><option value='Morocco' >Morocco</option><option value='Mozambique' >Mozambique</option><option value='Myanmar' >Myanmar</option><option value='Namibia' >Namibia</option><option value='Nauru' >Nauru</option><option value='Nepal' >Nepal</option><option value='Netherlands' >Netherlands</option><option value='Netherlands Antilles' >Netherlands Antilles</option><option value='New Caledonia' >New Caledonia</option><option value='New Zealand' >New Zealand</option><option value='Nicaragua' >Nicaragua</option><option value='Niger' >Niger</option><option value='Nigeria' >Nigeria</option><option value='Niue' >Niue</option><option value='Norfolk Island' >Norfolk Island</option><option value='Northern Mariana Islands' >Northern Mariana Islands</option><option value='Norway' >Norway</option><option value='Oman' >Oman</option><option value='Pakistan' >Pakistan</option><option value='Palau' >Palau</option><option value='Panama' >Panama</option><option value='Papua New Guinea' >Papua New Guinea</option><option value='Paraguay' >Paraguay</option><option value='Peru' >Peru</option><option value='Philippines' >Philippines</option><option value='Pitcairn' >Pitcairn</option><option value='Poland' >Poland</option><option value='Portugal' >Portugal</option><option value='Puerto Rico' >Puerto Rico</option><option value='Qatar' >Qatar</option><option value='Reunion' >Reunion</option><option value='Romania' >Romania</option><option value='Russian Federation' >Russian Federation</option><option value='Rwanda' >Rwanda</option><option value='Saint Helena' >Saint Helena</option><option value='Saint Kitts and Nevis' >Saint Kitts and Nevis</option><option value='Saint Lucia' >Saint Lucia</option><option value='Saint Pierre and Miquelon' >Saint Pierre and Miquelon</option><option value='Samoa' >Samoa</option><option value='San Marino' >San Marino</option><option value='Sao Tome and Principe' >Sao Tome and Principe</option><option value='Saudi Arabia' >Saudi Arabia</option><option value='Senegal' >Senegal</option><option value='Seychelles' >Seychelles</option><option value='Sierra Leone' >Sierra Leone</option><option value='Singapore' >Singapore</option><option value='Slovakia' >Slovakia</option><option value='Slovenia' >Slovenia</option><option value='Solomon Islands' >Solomon Islands</option><option value='Somalia' >Somalia</option><option value='South Africa' >South Africa</option><option value='Spain' >Spain</option><option value='Sri Lanka' >Sri Lanka</option><option value='Sth Georgia & Sth Sandwich Is' >Sth Georgia & Sth Sandwich Is</option><option value='St Vincent and the Grenadines' >St Vincent and the Grenadines</option><option value='Sudan' >Sudan</option><option value='Suriname' >Suriname</option><option value='Svalbard and Jan Mayen' >Svalbard and Jan Mayen</option><option value='Swaziland' >Swaziland</option><option value='Sweden' >Sweden</option><option value='Switzerland' >Switzerland</option><option value='Syrian Arab Republic' >Syrian Arab Republic</option><option value='Taiwan, Province of China' >Taiwan, Province of China</option><option value='Tajikistan' >Tajikistan</option><option value='Tanzania, United Republic of' >Tanzania, United Republic of</option><option value='Thailand' >Thailand</option><option value='Togo' >Togo</option><option value='Tokelau' >Tokelau</option><option value='Tonga' >Tonga</option><option value='Trinidad and Tobago' >Trinidad and Tobago</option><option value='Tunisia' >Tunisia</option><option value='Turkey' >Turkey</option><option value='Turkmenistan' >Turkmenistan</option><option value='Turks and Caicos Islands' >Turks and Caicos Islands</option><option value='Tuvalu' >Tuvalu</option><option value='Uganda' >Uganda</option><option value='Ukraine' >Ukraine</option><option value='United Arab Emirates' >United Arab Emirates</option><option value='United Kingdom' >United Kingdom</option><option value='Uruguay' >Uruguay</option><option value='US Minor Outlying Islands' >US Minor Outlying Islands</option><option value='Uzbekistan' >Uzbekistan</option><option value='Vanuatu' >Vanuatu</option><option value='Venezuela' >Venezuela</option><option value='Vietnam' >Vietnam</option><option value='Virgin Islands (British)' >Virgin Islands (British)</option><option value='Virgin Islands (U.S.)' >Virgin Islands (U.S.)</option><option value='Wallis and Futuna Islands' >Wallis and Futuna Islands</option><option value='Western Sahara' >Western Sahara</option><option value='Yemen' >Yemen</option><option value='Yugoslavia' >Yugoslavia</option><option value='Zambia' >Zambia</option><option value='Zimbabwe' >Zimbabwe</option></select></div></li><li id="field_1_8" class="gfield gfield--type-select sub-category gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_8" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_8'>Pick a category<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_select'><select name='input_8' id='input_1_8' class='large gfield_select' aria-required="true" aria-invalid="false" ><option value='' selected='selected' class='gf_placeholder'>Pick a category</option><option value='Accelerating Microscopy' >Accelerating Microscopy</option><option value='Advancing Materials' >Advancing Materials</option><option value='Advancing Mining' >Advancing Mining</option><option value='Analyzing Metals' >Analyzing Metals</option><option value='Behind the Bench' selected='selected'>Behind the Bench</option><option value='Examining Food' >Examining Food</option><option value='Identifying Threats' >Identifying Threats</option><option value='Life in the Lab' >Life in the Lab</option></select></div></li><li id="field_1_9" class="gfield gfield--type-checkbox gfield--type-choice gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_9" ><label class='gfield_label gform-field-label screen-reader-text gfield_label_before_complex' ><span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_checkbox'><ul class='gfield_checkbox' id='input_1_9'><li class='gchoice gchoice_1_9_1'> <input class='gfield-choice-input' name='input_9.1' type='checkbox' value='1' id='choice_1_9_1' /> <label for='choice_1_9_1' id='label_1_9_1' class='gform-field-label gform-field-label--type-inline'>I would like to receive information about content, events, products, services and promotions from Thermo Fisher Scientific and its affiliates. I agree for the Thermo Fisher Scientific group (thermofisher.com/legalentities) to contact me by email and/or telephone to inform me of events, products, services and/or promotions offered in connection with the brands Thermo Scientific™, Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent ™, Unity Lab Services™, Fisher Scientifc™. I can withdraw my consent and unsubscribe at any time by emailing reply@thermofisher.com By submitting my data, I give consent to the collection, processing and use of my personal data in accordance with the Thermo Fisher Scientific Policy (thermofisher.com/privacypolicy).</label> </li></ul></div></li><li id="field_1_10" class="gfield gfield--type-hidden gform_hidden field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_10" ><div class='ginput_container ginput_container_text'><input name='input_10' id='input_1_10' type='hidden' class='gform_hidden' aria-invalid="false" value='Behind the Bench' /></div></li><li id="field_1_11" class="gfield gfield--type-honeypot gform_validation_container field_sublabel_above gfield--has-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_11" ><label class='gfield_label gform-field-label' for='input_1_11'>Name</label><div class='ginput_container'><input name='input_11' id='input_1_11' type='text' value='' autocomplete='new-password'/></div><div class='gfield_description' id='gfield_description_1_11'>This field is for validation purposes and should be left unchanged.</div></li></ul></div> <div class='gform_footer top_label'> <input type='submit' id='gform_submit_button_1' class='gform_button button' value='Subscribe' onclick='if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} ' onkeypress='if( event.keyCode == 13 ){ if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} jQuery("#gform_1").trigger("submit",[true]); }' /> <input type='hidden' class='gform_hidden' name='is_submit_1' value='1' /> <input type='hidden' class='gform_hidden' name='gform_submit' value='1' /> <input type='hidden' class='gform_hidden' name='gform_unique_id' value='' /> <input type='hidden' class='gform_hidden' name='state_1' value='WyJbXSIsImFiYmI5YzY2YWJjNDlmYzYwZjUzMTBiZjQ0NGIwZGNmIl0=' /> <input type='hidden' class='gform_hidden' name='gform_target_page_number_1' id='gform_target_page_number_1' value='0' /> <input type='hidden' class='gform_hidden' name='gform_source_page_number_1' id='gform_source_page_number_1' value='1' /> <input type='hidden' name='gform_field_values' value='' /> </div> <p style="display: none !important;" class="akismet-fields-container" data-prefix="ak_"><label>Δ<textarea name="ak_hp_textarea" cols="45" rows="8" maxlength="100"></textarea></label><input type="hidden" id="ak_js_3" name="ak_js" value="182"/><script>document.getElementById( "ak_js_3" ).setAttribute( "value", ( new Date() ).getTime() );</script></p></form> </div><script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {gformInitSpinner( 1, 'https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery('#gform_ajax_frame_1').on('load',function(){var contents = jQuery(this).contents().find('*').html();var is_postback = contents.indexOf('GF_AJAX_POSTBACK') >= 0;if(!is_postback){return;}var form_content = jQuery(this).contents().find('#gform_wrapper_1');var is_confirmation = jQuery(this).contents().find('#gform_confirmation_wrapper_1').length > 0;var is_redirect = contents.indexOf('gformRedirect(){') >= 0;var is_form = form_content.length > 0 && ! is_redirect && ! is_confirmation;var mt = parseInt(jQuery('html').css('margin-top'), 10) + parseInt(jQuery('body').css('margin-top'), 10) + 100;if(is_form){jQuery('#gform_wrapper_1').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_1').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_1').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_1').val();gformInitSpinner( 1, 'https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [1, current_page]);window['gf_submitting_1'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = contents;}jQuery('#gform_wrapper_1').replaceWith(confirmation_content);jQuery(document).trigger('gform_confirmation_loaded', [1]);window['gf_submitting_1'] = false;wp.a11y.speak(jQuery('#gform_confirmation_message_1').text());}else{jQuery('#gform_1').append(contents);if(window['gformRedirect']) {gformRedirect();}}jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "current_page", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } );} ); /* ]]> */ </script> </div> </div></div><script>if(window.location.search.indexOf('post_id')!=-1){ window.close();}jQuery(document).ready(function ($) { $('p').mashSelectionSharer();});</script>  <script type="text/html" id="wpls--tmpl"> <li class="search-result"> <a href="<%= post.link %>"> <div class="wpls--item-title-wrap"> <h4 class="wpls--item-title"><%= post.title %></h4> </div> </a> </li> </script> <script type="text/javascript"> /* MonsterInsights Scroll Tracking */ if ( typeof(jQuery) !== 'undefined' ) { jQuery( document ).ready(function(){ function monsterinsights_scroll_tracking_load() { if ( ( typeof(__gaTracker) !== 'undefined' && __gaTracker && __gaTracker.hasOwnProperty( "loaded" ) && __gaTracker.loaded == true ) || ( typeof(__gtagTracker) !== 'undefined' && __gtagTracker ) ) { (function(factory) { factory(jQuery); }(function($) { /* Scroll Depth */ "use strict"; var defaults = { percentage: true }; var $window = $(window), cache = [], scrollEventBound = false, lastPixelDepth = 0; /* * Plugin */ $.scrollDepth = function(options) { var startTime = +new Date(); options = $.extend({}, defaults, options); /* * Functions */ function sendEvent(action, label, scrollDistance, timing) { if ( 'undefined' === typeof MonsterInsightsObject || 'undefined' === typeof MonsterInsightsObject.sendEvent ) { return; } var paramName = action.toLowerCase(); var fieldsArray = { send_to: 'G-K4QTN4KM25', non_interaction: true }; fieldsArray[paramName] = label; if (arguments.length > 3) { fieldsArray.scroll_timing = timing MonsterInsightsObject.sendEvent('event', 'scroll_depth', fieldsArray); } else { MonsterInsightsObject.sendEvent('event', 'scroll_depth', fieldsArray); } } function calculateMarks(docHeight) { return { '25%' : parseInt(docHeight * 0.25, 10), '50%' : parseInt(docHeight * 0.50, 10), '75%' : parseInt(docHeight * 0.75, 10), /* Cushion to trigger 100% event in iOS */ '100%': docHeight - 5 }; } function checkMarks(marks, scrollDistance, timing) { /* Check each active mark */ $.each(marks, function(key, val) { if ( $.inArray(key, cache) === -1 && scrollDistance >= val ) { sendEvent('Percentage', key, scrollDistance, timing); cache.push(key); } }); } function rounded(scrollDistance) { /* Returns String */ return (Math.floor(scrollDistance/250) * 250).toString(); } function init() { bindScrollDepth(); } /* * Public Methods */ /* Reset Scroll Depth with the originally initialized options */ $.scrollDepth.reset = function() { cache = []; lastPixelDepth = 0; $window.off('scroll.scrollDepth'); bindScrollDepth(); }; /* Add DOM elements to be tracked */ $.scrollDepth.addElements = function(elems) { if (typeof elems == "undefined" || !$.isArray(elems)) { return; } $.merge(options.elements, elems); /* If scroll event has been unbound from window, rebind */ if (!scrollEventBound) { bindScrollDepth(); } }; /* Remove DOM elements currently tracked */ $.scrollDepth.removeElements = function(elems) { if (typeof elems == "undefined" || !$.isArray(elems)) { return; } $.each(elems, function(index, elem) { var inElementsArray = $.inArray(elem, options.elements); var inCacheArray = $.inArray(elem, cache); if (inElementsArray != -1) { options.elements.splice(inElementsArray, 1); } if (inCacheArray != -1) { cache.splice(inCacheArray, 1); } }); }; /* * Throttle function borrowed from: * Underscore.js 1.5.2 * http://underscorejs.org * (c) 2009-2013 Jeremy Ashkenas, DocumentCloud and Investigative Reporters & Editors * Underscore may be freely distributed under the MIT license. */ function throttle(func, wait) { var context, args, result; var timeout = null; var previous = 0; var later = function() { previous = new Date; timeout = null; result = func.apply(context, args); }; return function() { var now = new Date; if (!previous) previous = now; var remaining = wait - (now - previous); context = this; args = arguments; if (remaining <= 0) { clearTimeout(timeout); timeout = null; previous = now; result = func.apply(context, args); } else if (!timeout) { timeout = setTimeout(later, remaining); } return result; }; } /* * Scroll Event */ function bindScrollDepth() { scrollEventBound = true; $window.on('scroll.scrollDepth', throttle(function() { /* * We calculate document and window height on each scroll event to * account for dynamic DOM changes. */ var docHeight = $(document).height(), winHeight = window.innerHeight ? window.innerHeight : $window.height(), scrollDistance = $window.scrollTop() + winHeight, /* Recalculate percentage marks */ marks = calculateMarks(docHeight), /* Timing */ timing = +new Date - startTime; checkMarks(marks, scrollDistance, timing); }, 500)); } init(); }; /* UMD export */ return $.scrollDepth; })); jQuery.scrollDepth(); } else { setTimeout(monsterinsights_scroll_tracking_load, 200); } } monsterinsights_scroll_tracking_load(); }); } /* End MonsterInsights Scroll Tracking */ </script><script type="text/javascript" id="wpls-script-js-extra"> /* <![CDATA[ */ var wp_search_vars = {"helperText":"one more character","api":"https:\/\/admin.acceleratingscience.com\/wp-json\/as\/v1\/all-posts\/9"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/wp-search-live/public/assets/js/wp-live-search.js?ver=0.9" id="wpls-script-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/comment-reply.min.js?ver=6.6.1" id="comment-reply-js" async="async" data-wp-strategy="async"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/dom-ready.min.js?ver=f77871ff7694fffea381" id="wp-dom-ready-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/hooks.min.js?ver=2810c76e705dd1a53b18" id="wp-hooks-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/i18n.min.js?ver=5e580eb46a90c2b997e6" id="wp-i18n-js"></script> <script type="text/javascript" id="wp-i18n-js-after"> /* <![CDATA[ */ wp.i18n.setLocaleData( { 'text direction\u0004ltr': [ 'ltr' ] } ); /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/a11y.min.js?ver=d90eebea464f6c09bfd5" id="wp-a11y-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/js/placeholders.jquery.min.js?ver=2.8.17" id="gform_placeholder-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/assets/js/dist/vendor-theme.min.js?ver=54e7080aa7a02c83aa61fae430b9d869" id="gform_gravityforms_theme_vendors-js"></script> <script type="text/javascript" id="gform_gravityforms_theme-js-extra"> /* <![CDATA[ */ var gform_theme_config = {"common":{"form":{"honeypot":{"version_hash":"4a792b070322e711c2521ee9802c04ea"}}},"hmr_dev":"","public_path":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/plugins\/gravityforms\/assets\/js\/dist\/"}; /* ]]> */ </script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/gravityforms/assets/js/dist/scripts-theme.min.js?ver=bab19fd84843dabc070e73326d787910" id="gform_gravityforms_theme-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/ui/core.min.js?ver=1.13.3" id="jquery-ui-core-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/ui/tabs.min.js?ver=1.13.3" id="jquery-ui-tabs-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/themes/accelerating-science-blogs/js/scripts.js?ver=1.2.9.3" id="scripts-js"></script> <script type="text/javascript" id="boxzilla-js-extra"> /* <![CDATA[ */ var boxzilla_options = {"testMode":"","boxes":[{"id":10626,"icon":"×","content":"","css":{"position":"bottom-right"},"trigger":{"method":"percentage","value":35},"animation":"fade","cookie":{"triggered":0,"dismissed":0},"rehide":false,"position":"bottom-right","screenWidthCondition":null,"closable":true,"post":{"id":10626,"title":"Single Call to Action","slug":"single-call-to-action"}}]}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/boxzilla/assets/js/script.js?ver=3.3.1" id="boxzilla-js"></script> <script defer type="text/javascript" src="https://www.thermofisher.com/blog/behindthebench/wp-content/plugins/akismet/_inc/akismet-frontend.js?ver=1722950842" id="akismet-frontend-js"></script> <script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() { jQuery(document).on('gform_post_render', function(event, formId, currentPage){if(formId == 1) {gformInitChosenFields('#input_1_7,#input_1_8','No results matched');if(typeof Placeholders != 'undefined'){ Placeholders.enable(); }} } );jQuery(document).on('gform_post_conditional_logic', function(event, formId, fields, isInit){gformInitChosenFields('#input_1_7,#input_1_8','No results matched');} ) } ); /* ]]> */ </script> <script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "1", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, 1] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: 1 } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } ); /* ]]> */ </script> <script type="text/javascript" src="/K1tG87Y8lf3h/SMnlrP/_GYOkD/LOVucQL4wVLzt1/YxxmKQ/CQB/oTCJAXxg"></script></body></html>