CINXE.COM
Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model - Peeref
<!DOCTYPE html> <html lang="en"> <head> <link rel="dns-prefetch" href="//www.peeref.com"> <link rel="dns-prefetch" href="//peeref-open.s3.amazonaws.com"> <link rel="dns-prefetch" href="//www.w3.org"> <link rel="dns-prefetch" href="//doi.org"> <link rel="dns-prefetch" href="//www.linkedin.com"> <link rel="dns-prefetch" href="//twitter.com"> <link rel="dns-prefetch" href="//api.qrserver.com"> <link rel="dns-prefetch" href="//www.facebook.com"> <link rel="dns-prefetch" href="//www.googletagmanager.com"> <meta charset="utf-8"> <title>Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model - Peeref</title> <meta http-equiv="X-UA-Compatible" content="IE=edge, chrome=1"> <meta name="keywords" content="manuscript, research article, comment, full text"> <meta name="description" content="Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=no" /> <meta property="og:locale" content="en"> <meta property="og:title" content="Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model - Peeref"> <meta property="og:description" content="Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model"> <meta property="og:url" content="https://www.peeref.com/works/83645405"> <meta property="og:site_name" content="Peeref"> <meta property="og:image" content=""> <meta name="csrf-token" content="Y4TJ6cuFVsvL1xHl6GEeATL1ssVNM1BbbMZGUbEP"> <meta http-equiv="Cache-Control" content="no-transform" /> <meta http-equiv="Cache-Control" content="no-siteapp" /> <link rel="shortcut icon" href="https://www.peeref.com/favicon.ico"> <link href="https://peeref-open.s3.amazonaws.com/css/frameworks.bd380c8996823ba04442.css" rel="stylesheet"> <link href="https://peeref-open.s3.amazonaws.com/css/app.3cfd9992ceaf352e0491.css" rel="stylesheet"> <script> if(window.self !== window.top) { location.href = '/static/html/forbidden.html'; } </script> <script> window.Config = { token: "Y4TJ6cuFVsvL1xHl6GEeATL1ssVNM1BbbMZGUbEP", url: "https://www.peeref.com/works/83645405", lang: "en", prefix: "", isMobile: "", assetDomain: "https://peeref-open.s3.amazonaws.com/" }; </script> </head> <body> <div id="app"> <div class="bg-warning position-relative w-100 text-center py-2" style="top: 0; z-index: 100000;"> <strong class="text-danger">Peeref no longer supports Internet Explorer. </strong> <a href="/static/html/browser.html">Please upgrade your browser.</a> </div> <nav id="nav" class="navbar navbar-default navbar-static-top navbar-inverse"> <div class="container"> <div class="navbar-header"> <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#app-navbar-collapse" aria-expanded="false"> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> <a class="navbar-brand p-3" href="https://www.peeref.com"> <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" width="108px" viewBox="0 0 1052 302"><g fill="#FFF"><path d="M227.6,92.9c5.2,13,17.9,22.1,32.7,22.1c19.4,0,35.2-15.8,35.2-35.2s-15.8-35.2-35.2-35.2 c-19.4,0-35.2,15.8-35.2,35.2c0,2.3,0.2,4.5,0.6,6.7l-98.1,24.6L154,59c3.2,1.1,6.6,1.8,10.1,1.8c16.4,0,29.7-13.3,29.7-29.7c0-16.4-13.3-29.7-29.7-29.7s-29.7,13.3-29.7,29.7c0,10.5,5.4,19.7,13.6,25l-27.9,55.1L90.5,94.2c1.4-3.2,2.1-6.8,2.1-10.6 c0-14.9-12.1-27-27-27c-14.9,0-27,12.1-27,27c0,14.9,12.1,27,27,27c8.8,0,16.6-4.2,21.6-10.7l28.5,16.3l-62.2,49.9 c-5.6-5.3-13.1-8.6-21.4-8.6c-17.1,0-30.9,13.8-30.9,30.9c0,17.1,13.8,30.9,30.9,30.9S63,205.4,63,188.4c0-6.3-1.9-12.3-5.2-17.2 l60-48.1l-7.5,106.4c-19.1,0.6-34.4,16.2-34.4,35.5c0,19.6,15.9,35.5,35.5,35.5c19.6,0,35.5-15.9,35.5-35.5c0-17.7-13-32.4-30-35.1 l7.6-107.5l87,67.9c-4.1,6.1-6.4,13.5-6.4,21.4c0,21.4,17.4,38.8,38.8,38.8s38.8-17.4,38.8-38.8c0-21.4-17.4-38.8-38.8-38.8 c-11.1,0-21.2,4.7-28.2,12.2L129,117.6L227.6,92.9z"></path></g><g fill="#FFF"><path d="M368.2,232.3V70.6h52.4c19.9,0,32.8,0.8,38.8,2.4c9.3,2.4,17,7.7,23.3,15.8c6.2,8.1,9.4,18.6,9.4,31.5c0,9.9-1.8,18.3-5.4,25c-3.6,6.8-8.2,12.1-13.7,15.9c-5.6,3.9-11.2,6.4-16.9,7.7c-7.8,1.5-19.1,2.3-33.9,2.3h-21.3v61H368.2z M400.8,98v45.9h17.9c12.9,0,21.5-0.8,25.8-2.5c4.3-1.7,7.7-4.3,10.2-7.9c2.5-3.6,3.7-7.8,3.7-12.6c0-5.9-1.7-10.7-5.2-14.6c-3.5-3.8-7.8-6.2-13.1-7.2c-3.9-0.7-11.7-1.1-23.5-1.1H400.8z"></path><path d="M586.5,195l30.9,5.2c-4,11.3-10.2,19.9-18.8,25.9c-8.6,5.9-19.3,8.9-32.2,8.9c-20.4,0-35.4-6.7-45.2-20c-7.7-10.7-11.6-24.1-11.6-40.4c0-19.4,5.1-34.6,15.2-45.6c10.1-11,23-16.5,38.5-16.5c17.4,0,31.2,5.8,41.3,17.3c10.1,11.5,14.9,29.1,14.5,52.9h-77.7c0.2,9.2,2.7,16.3,7.5,21.5c4.8,5.1,10.7,7.7,17.9,7.7c4.9,0,8.9-1.3,12.2-4S584.8,200.9,586.5,195z M588.3,163.7c-0.2-9-2.5-15.8-6.9-20.5c-4.4-4.7-9.8-7-16.1-7c-6.8,0-12.4,2.5-16.8,7.4c-4.4,4.9-6.6,11.6-6.5,20.1H588.3z"></path><path d="M712.2,195l30.9,5.2c-4,11.3-10.2,19.9-18.8,25.9c-8.6,5.9-19.3,8.9-32.2,8.9c-20.4,0-35.4-6.7-45.2-20c-7.7-10.7-11.6-24.1-11.6-40.4c0-19.4,5.1-34.6,15.2-45.6c10.1-11,23-16.5,38.5-16.5c17.4,0,31.2,5.8,41.3,17.3c10.1,11.5,14.9,29.1,14.5,52.9H667c0.2,9.2,2.7,16.3,7.5,21.5c4.8,5.1,10.7,7.7,17.9,7.7c4.9,0,8.9-1.3,12.2-4S710.5,200.9,712.2,195z M713.9,163.7c-0.2-9-2.5-15.8-6.9-20.5c-4.4-4.7-9.8-7-16.1-7c-6.8,0-12.4,2.5-16.8,7.4c-4.4,4.9-6.6,11.6-6.5,20.1H713.9z"></path><path d="M799.6,232.3h-31V115.2h28.8v16.7c4.9-7.9,9.4-13.1,13.3-15.6c3.9-2.5,8.4-3.8,13.4-3.8c7.1,0,13.9,1.9,20.4,5.8l-9.6,27c-5.2-3.4-10.1-5.1-14.6-5.1c-4.3,0-8,1.2-11,3.6c-3,2.4-5.4,6.7-7.1,13c-1.7,6.3-2.6,19.3-2.6,39.3V232.3z"></path><path d="M925.7,195l30.9,5.2c-4,11.3-10.2,19.9-18.8,25.9c-8.6,5.9-19.3,8.9-32.2,8.9c-20.4,0-35.4-6.7-45.2-20c-7.7-10.7-11.6-24.1-11.6-40.4c0-19.4,5.1-34.6,15.2-45.6c10.1-11,23-16.5,38.5-16.5c17.4,0,31.2,5.8,41.3,17.3c10.1,11.5,14.9,29.1,14.5,52.9h-77.7c0.2,9.2,2.7,16.3,7.5,21.5c4.8,5.1,10.7,7.7,17.9,7.7c4.9,0,8.9-1.3,12.2-4S924,200.9,925.7,195z M927.5,163.7c-0.2-9-2.5-15.8-6.9-20.5c-4.4-4.7-9.8-7-16.1-7c-6.8,0-12.4,2.5-16.8,7.4c-4.4,4.9-6.6,11.6-6.5,20.1H927.5z"></path><path d="M970,115.2h17.2v-8.8c0-9.9,1-17.2,3.1-22.1c2.1-4.9,6-8.8,11.6-11.9c5.6-3.1,12.7-4.6,21.3-4.6c8.8,0,17.5,1.3,25.9,4l-4.2,21.6c-4.9-1.2-9.7-1.8-14.2-1.8c-4.5,0-7.7,1-9.7,3.1c-1.9,2.1-2.9,6.1-2.9,12.1v8.3h23.2v24.4h-23.2v92.8h-31v-92.8H970V115.2z"></path></g></svg> </a> </div> <ul class="collapse navbar-collapse mb-0" id="app-navbar-collapse"> <ul class="nav navbar-nav"> <li class="dropdown dropdown-hover"> <a href="javascript:">Publish <span class="caret"></span></a> <ul class="dropdown-menu dropdown-menu-inverse"> <li><a href="https://www.peeref.com/posters">Posters</a></li> <li><a href="https://www.peeref.com/abstracts">Abstracts</a></li> </ul> </li> <li class="dropdown dropdown-hover"> <a href="javascript:">Discuss <span class="caret"></span></a> <ul class="dropdown-menu dropdown-menu-inverse"> <li><a href="https://www.peeref.com/hubs">Hubs</a></li> <li><a href="https://www.peeref.com/questions">Questions</a></li> </ul> </li> <li><a href="https://www.peeref.com/works">Papers</a></li> <li><a href="https://www.peeref.com/webinars">Webinars</a></li> <li><a href="https://www.peeref.com/funding">Funding</a></li> <li class="dropdown dropdown-hover"> <a href="javascript:">Publishing Tools <span class="caret"></span></a> <ul class="dropdown-menu dropdown-menu-inverse"> <li><a href="https://www.peeref.com/journals">Journals</a></li> <li><a href="https://www.peeref.com/connect">Connect</a></li> <li><a href="https://www.peeref.com/e-collections">Collections</a></li> <li><a href="https://www.peeref.com/reviewer">Reviewers</a></li> </ul> </li> <li class="dropdown dropdown-hover"> <a href="javascript:">Events <span class="caret"></span></a> <ul class="dropdown-menu dropdown-menu-inverse"> <li><a href="https://www.peeref.com/poster-competition">Poster Challenger</a></li> <li><a href="https://www.peeref.com/webinar-competition">Video Presentation</a></li> <li><a href="https://www.peeref.com/artwork-competition">Art of Science</a></li> <li><a href="https://www.peeref.com/events/reviewer-roulette">Reviewer Roulette</a></li> </ul> </li> </ul> <ul id="auth-menu" class="nav navbar-nav navbar-right" style="display: inline-flex; align-items: center;"> <li> <a href="https://www.peeref.com/login"> <strong>My Account</strong> </a> </li> </ul> <ul class="nav navbar-nav navbar-right" style="display: inline-flex; align-items: center; margin-left: 20px;"> <li id="language" class="d-none d-xl-inline-flex"> <a href="javascript:"> <div class="current"> <i class="ivu-icon ivu-icon-md-globe"></i> EN </div> </a> <div class="selection"> <a rel="alternate" hreflang="en" href="https://www.peeref.com/works/83645405" > <span>English</span> </a> <a rel="alternate" hreflang="zh" href="https://www.peeref.com/zh/works/83645405" > <span>中文</span> </a> </div> </li> </ul> </ul> </div> </nav> <main> <div id="top-info-banner" class="container-fluid mb-0"> <div class="container"> <div class="d-flex align-items-center" style="margin-top: 30px;"> <span class="text-white"> <strong class="f18">☆</strong> <span class="f16">4.5</span> </span> <span class="mx-3"></span> <span class="tag">Article</span> </div> <h1 class="title title-for-article"> Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model </h1> <div class="help-links-left"> <p class="pub-info"> FORENSIC SCIENCE INTERNATIONAL-GENETICS (2024) </p> </div> </div> </div> <div id="article-sticky-navbar"> <div class="container"> <div class="d-flex justify-content-between flex-wrap flex-md-nowrap"> <div class="d-flex align-items-center mb-2"> <ul class="nav nav-underline f16 font-weight-bold"> <li class="active"> <a href="javascript:;"> Overview </a> </li> <li class=""> <a href="https://www.peeref.com/works/83645405/comments"> Write a Review </a> </li> </ul> </div> <div class="d-flex align-items-center justify-content-md-end flex-wrap flex-md-nowrap"> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://doi.org/10.1016/j.fsigen.2024.103098" target="_blank" class="btn btn-warning btn-circle"> <i class="ivu-icon ivu-icon-md-copy f16"></i> <strong>Get Full Text</strong> </a> </div> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://www.peeref.com/works/83645405/add-to-collection" class="btn btn-success btn-circle"> <strong>Add to Collection</strong> </a> </div> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <button class="btn btn-success btn-circle" id="reading-btn"> <strong>Further Reading</strong> </button> </div> <div class="flex-shrink-0 mt-3 mt-md-0"> <div class="dropdown"> <button class="font-weight-bold f24 ivu-btn ivu-btn-default ivu-btn-circle ivu-btn-large ivu-btn-icon-only dropdown-toggle" data-toggle="dropdown"> <i class="ivu-icon ivu-icon-md-more"></i> </button> <ul class="dropdown-menu dropdown-menu-right"> <li> <a href="#" data-target="#export-citation" data-toggle="modal"> <i class="ivu-icon ivu-icon-md-quote text-muted mr-1"></i> Export Citation </a> </li> <li> <a href="#" data-target="#share-paper" data-toggle="modal"> <i class="ivu-icon ivu-icon-md-share-alt text-muted mr-1"></i> Share Paper </a> </li> <li> <a href="https://www.peeref.com/works/83645405/references"> <i class="ivu-icon ivu-icon-md-list text-muted mr-1"></i> References </a> </li> </ul> </div> </div> </div> </div> </div> </div> <div id="article-details" class="container"> <div class="col-md-4 px-0 pr-md-3"> <div class="f15 panel-box rounded shadow-none border"> <div class="mb-3 pb-3"> <h4 class="mt-0">Journal</h4> <div class="f16"> <h5 class="title f16"> <a href="https://www.peeref.com/journals/2908/forensic-science-international-genetics"> FORENSIC SCIENCE INTERNATIONAL-GENETICS </a> </h5> <span> Volume 73, Issue -, Pages - </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Publisher</h4> <div class="f16"> <h5 class="title f16 text-primary"> ELSEVIER IRELAND LTD </h5> <div class="my-2"> DOI: 10.1016/j.fsigen.2024.103098 </div> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Keywords</h4> <div class="f16"> RT-RPA; CRISPR-Cas; LFA; ALAS2; Blood Identification </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Categories</h4> <div class="f16"> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Genetics+%26+Heredity" target="_blank" class="text-dark btn btn-link p-0 text-left"> Genetics & Heredity </a> </span> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Medicine%2C+Legal" target="_blank" class="text-dark btn btn-link p-0 text-left"> Medicine, Legal </a> </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Funding</h4> <div class="f16"> <ol class=""> <li>National Science and Technology Council [MOST 110-2320-B-015-001, MOST 111-2320-B-015-001-MY2]</li> </ol> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 text-center">Ask authors/readers for more resources</h4> <div class="requests"> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/file.png" alt=""> </div> <h4>Protocol</h4> <p> <a href="https://www.peeref.com/works/83645405/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/experiment.png" alt=""> </div> <h4>Reagent</h4> <p> <a href="https://www.peeref.com/works/83645405/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> </div> </div> </div> <div class="col-md-8 px-0 pl-md-3"> <div id="article-summary-panel" class="mb-4"> <ul class="nav nav-tabs" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#ai_summary" data-toggle="tab" class="summary-tab mx-0 f16 text-dark"> <strong>Automated Summary</strong> <strong class="text-danger ml-1"><i>New</i></strong> </a> </li> <li class=""> <a href="#raw_abstract" data-toggle="tab" class="abstract-tab mx-0 f16 text-dark"> <strong>Abstract</strong> </a> </li> </ul> <div class="tab-content border border-top-0"> <div id="ai_summary" class="tab-pane active"> <div class="summary-panel panel-box mb-0 rounded shadow-none"> <div class="f16">A blood test method based on recombinase polymerase amplification, CRISPR-Cas, and lateral flow assay is reported, which is rapid, sensitive, and specific, and can detect the ALAS2 marker in blood. The test can use extracted RNA or directly added body fluids as templates, with a low detection limit, and can only detect blood, not other body fluids. When peripheral blood is mixed with saliva or semen, the test results will be affected. This method is expected to be applied in places far from the laboratory, such as crime scenes.</div> </div> </div> <div id="raw_abstract" class="tab-pane "> <div class="abstract-panel panel-box mb-0 rounded shadow-none"> <div class="f16">A rapid, sensitive and specific test for blood is reported based on a novel application of recombinase polymerase amplification integrated with CRISPR-Cas and lateral flow assay (LFA). The blood specific marker ALAS2 was used as the target to record the presence of blood. The assay used either RNA extracted from a body fluid as a template, or omitting this extraction step and using a direct approach where the questioned body fluid was added directly to the assay. The assay only detected blood (all peripheral blood and some menstrual blood samples) and no other body fluid (semen, saliva, or vaginal fluid). The limit of detection varied from an initial template of 0.195 ng extracted RNA (27 dilution) or 0.0218 mu L (26 dilution) liquid peripheral blood. The assay gave the expected result when peripheral blood was mixed with saliva: ratios of peripheral blood/saliva at 19:1, 3:1, 1:1, 1:3 and 1:19 all gave a positive result using extracted RNA. By contrast, only three ratios of peripheral blood and saliva gave a positive result for blood (19:1, 3:1 and 1:1) when adding these two body fluids directly. When peripheral blood was mixed with semen there was a strong inhibition of the assay and ALAS2 could only be detected at ratio of 19:1 using RNA. Using reconstituted peripheral bloodstains gave comparable results to liquid peripheral blood. This is the first application of RT-RPA integrated CRISPR and combined with a LFA assay to detect body fluid-specific RNA. The proposed method opens up the potential to perform this method remote from laboratories such as at crime scenes.</div> </div> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Authors</h4> <div class="mb-3"> <article-authors tid="83645405" list="[{"name":"Chih-Wen Su","sequence":1},{"name":"Yi-Che Hsu","sequence":2},{"name":"Li-Chin Tsai","sequence":3},{"name":"James Chun- Lee","sequence":4},{"name":"Adrian Linacre","sequence":5},{"name":"Hsing-Mei Hsieh","sequence":6}]" verified="[]" page="work" ></article-authors> </div> <div class="alert alert-warning mb-0"> <h5 class="mt-0 bg-warning text-dark px-3 rounded d-inline-block"> I am an author on this paper </h5> <div class="font-weight-bold f13"> Click your name to claim this paper and add it to your profile. </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Reviews</h4> <div class="d-flex flex-wrap flex-md-nowrap"> <div class="flex-grow-1"> <h4 class="f16"> Primary Rating <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="The primary rating indicates the level of overall quality for the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap align-items-center alert mb-0"> <div class="d-flex align-items-center justify-content-center"> <Rate disabled allow-half value="4.5" style="font-size: 28px;"></Rate> <strong class="f20 m-3" style="color: #f5a623;">4.5</strong> </div> <div class="text-muted mx-4"> Not enough ratings </div> </div> <h4 class="f16"> Secondary Ratings <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="Secondary ratings independently reflect strengths or weaknesses of the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap alert"> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Novelty</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Significance</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Scientific rigor</h5> <strong class="mx-4">-</strong> </div> </div> </div> <div class="flex-shrink-0"> <div class="border bg-light py-2 px-4"> <h5 class="mb-1">Rate this paper</h5> <Rate class="f24" @on-change="function(value){ location.href='https://www.peeref.com/works/83645405/comments?rating='+value }"></Rate> </div> </div> </div> </div> <div id="collection" class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Recommended</h4> <div class="my-3"> <ul class="nav nav-pills border-bottom pb-3" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#articles_from_related" data-toggle="tab" class="mx-0 f15"> <strong>Related</strong> </a> </li> <li class=""> <a href="#articles_from_authors" data-toggle="tab" class="mx-0 f15"> <strong>From Same Authors</strong> </a> </li> <li class=""> <a href="#articles_from_journal" data-toggle="tab" class="mx-0 f15"> <strong>From Same Journal</strong> </a> </li> </ul> <div class="tab-content"> <div id="articles_from_related" class="tab-pane active"> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Food Science & Technology </span> </div> <h4> <a href="https://www.peeref.com/works/82920490" class="text-dark hover-underline">RT-RPA and RPA-LFA assay for rapid and ultrasensitive detection of Vibrio parahaemolyticus</a> </h4> <p class="text-ellipsis-2">Antuo Hu, Huan Chen, Changzheng Shi, Zhaoxin Lu, Fanqiang Meng, Xiaomei Bie</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2893.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed two rapid detection methods based on RPA technology, RT-RPA and RPA-LFA, using two new specific targets of Vibrio parahaemolyticus. These methods can detect V. parahaemolyticus within 20-25 minutes with high specificity and sensitivity, and a dual RT-RPA and dual RPA-LFA method was also constructed. These methods are significant for food safety emergency response. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FOOD CONTROL</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82920490/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Infectious Diseases </span> </div> <h4> <a href="https://www.peeref.com/works/82032644" class="text-dark hover-underline">CRISPR-Cas based diagnostic tools: Bringing diagnosis out of labs</a> </h4> <p class="text-ellipsis-2">Abu Sufiyan Chhipa, Ekta Radadiya, Snehal Patel</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2290.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article discusses the applicability of the CRISPR-Cas system in the detection of infectious diseases. The system combines the advantages of PCR and antigen detection techniques, with high sensitivity and specificity. Limitations and future research directions are also pointed out. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82032644/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Microbiology </span> </div> <h4> <a href="https://www.peeref.com/works/81758923" class="text-dark hover-underline">RPA-CRISPR/Cas12a-LFA combined with a digital visualization instrument to detect Toxoplasma gondii in stray dogs and cats in Zhejiang province, China</a> </h4> <p class="text-ellipsis-2">Hao Sun, Jiyuan Fan, Hongkun Chu, Yafan Gao, Jiawen Fang, Qinli Wu, Haojie Ding, Xunhui Zhuo, Qingming Kong, Hangjun Lv, Bin Zheng, Shaohong Lu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/10633.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed an RPA-CRISPR/Cas12a assay for the detection of Toxoplasma gondii, which is highly accurate and sensitive, and can detect T. gondii within 55 minutes without cross-reactivity with other parasites. The assay was combined with a digital visualization instrument to reduce false-negative results. The researchers validated the assay by establishing a mouse model and investigating the prevalence of T. gondii in stray cats and dogs in Zhejiang province. The assay is promising for on-site surveillance and early diagnosis of T. gondii infection. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">MICROBIOLOGY SPECTRUM</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81758923/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Environmental Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/26066029" class="text-dark hover-underline">Generation and application of a novel high-throughput detection based on RPA-CRISPR technique to sensitively monitor pathogenic microorganisms in the environment</a> </h4> <p class="text-ellipsis-2">Li Liu, Jin-Jing Duan, Xing-Yi Wei, Huan Hu, Yuan-Bo Wang, Pan-Pan Jia, De-Sheng Pei</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7412.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a high-throughput and highly sensitive method for monitoring Staphylococcus aureus in water using the RPA-CRISPR/Cas12a detection system was developed. This method combines nucleic acid amplification and the activity of the CRISPR/Cas12a system to achieve rapid and accurate detection of S. aureus in water samples within 35 minutes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE OF THE TOTAL ENVIRONMENT</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26066029/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/82382536" class="text-dark hover-underline">Identification of Viruses Infecting Phalaenopsis Orchids Using Nanopore Sequencing and Development of an RT-RPA-CRISPR/Cas12a for Rapid Visual Detection of Nerine Latent Virus</a> </h4> <p class="text-ellipsis-2">Hyo-Jeong Lee, Hae-Jun Kim, In-Sook Cho, Rae-Dong Jeong</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/3812.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study used MinION to detect viruses in Phalaenopsis orchids and developed the RT-RPA-CRISPR/Cas12a method for rapid and accurate diagnosis of NeLV infection, with high sensitivity, providing a new strategy for orchid virus disease detection. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82382536/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biophysics </span> </div> <h4> <a href="https://www.peeref.com/works/82532966" class="text-dark hover-underline">Sensitive and specific CRISPR-Cas12a assisted nanopore with RPA for Monkeypox detection</a> </h4> <p class="text-ellipsis-2">Md. Ahasan Ahamed, Muhammad Asad Ullah Khalid, Ming Dong, Anthony J. Politza, Zhikun Zhang, Aneesh Kshirsagar, Tianyi Liu, Weihua Guan</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1203.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed a highly sensitive and specific isothermal RPA-SCAN method for monkeypox virus detection, which addresses the obstacles of PCR-SCAN in point-of-care applications and has the potential to detect other infectious pathogens. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOSENSORS & BIOELECTRONICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82532966/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Environmental Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/81770426" class="text-dark hover-underline">Revolutionizing aquatic eco-environmental monitoring: Utilizing the RPA-Cas-FQ detection platform for zooplankton</a> </h4> <p class="text-ellipsis-2">Huan Hu, Li Liu, Xing-Yi Wei, Jin-Jing Duan, Jiao-Yun Deng, De-Sheng Pei</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7412.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The RPA-CRISPR/Cas technology has great advantages in molecular diagnostics and pathogen detection, but its application potential in aquatic ecosystem monitoring has not been fully explored. In this paper, using zooplankton as a model, we optimized the RPA-CRISPR/Cas12a fluorescence detection platform to achieve rapid and accurate qualitative and semi-quantitative detection, and developed a simple identification procedure for zooplankton. The platform showed strong performance in the detection of real environmental DNA samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE OF THE TOTAL ENVIRONMENT</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81770426/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/81952131" class="text-dark hover-underline">Novel CRISPR/SpRY system for rapid detection of CRISPR/Cas-mediated gene editing in rice</a> </h4> <p class="text-ellipsis-2">Zhixun Su, Xiaofu Wang, Xiaoyun Chen, Lin Ding, Xiaoqun Zeng, Junfeng Xu, Cheng Peng</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/514.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed a novel and efficient method, the CRISPR/SpRY detection platform, for rapid screening of CRISPR/Cas9-induced mutants. The platform is based on CRISPR/SpRY-mediated in vitro cleavage and was validated using genetically edited rice samples. The method is simple to operate, instrument-free, cost-effective, and time-efficient, and can detect various types of mutations with a detection limit as low as 1%. The significance of this platform lies in its liberation from the limitations of protospacer adjacent motif sequences, expanding the scope and versatility of CRISPR-based detection platforms. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICA CHIMICA ACTA</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81952131/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Virology </span> </div> <h4> <a href="https://www.peeref.com/works/81801727" class="text-dark hover-underline">On-Site and Visual Detection of the H5 Subtype Avian Influenza Virus Based on RT-RPA and CRISPR/Cas12a</a> </h4> <p class="text-ellipsis-2">Xu Zhou, Siwen Wang, Yue Ma, Yongping Jiang, Yanbing Li, Jianzhong Shi, Guohua Deng, Guobin Tian, Huihui Kong, Xiurong Wang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/8207.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed a novel H5 virus detection method using CRISPR/Cas12a and RT-RPA technologies, which can directly visualize the detection results, accurately identify H5 viruses with high specificity, no cross-reactivity, low detection limit, comparable and slightly superior to RT-qPCR, and high positive detection rate in clinical samples. This method has high sensitivity, specificity, and field applicability, and can be used as an effective tool for the early detection and management of H5 subtype AIV outbreaks. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">VIRUSES-BASEL</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81801727/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Multidisciplinary </span> </div> <h4> <a href="https://www.peeref.com/works/26182969" class="text-dark hover-underline">Endonuclease-Assisted PAM-free Recombinase Polymerase Amplification Coupling with CRISPR/Cas12a (E-PfRPA/Cas) for Sensitive Detection of DNA Methylation</a> </h4> <p class="text-ellipsis-2">Shiying Zhou, Jiangbo Dong, Liyuan Deng, Guixue Wang, Mei Yang, Yongzhong Wang, Danqun Huo, Changjun Hou</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/10311.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The proposed method combines endonucleases, CRISPR/Cas, and recombinases to detect DNA methylation with high sensitivity. It shows great potential for the analysis of tumor-specific genes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ACS SENSORS</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26182969/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Virology </span> </div> <h4> <a href="https://www.peeref.com/works/33936922" class="text-dark hover-underline">Rapid detection of avian influenza virus based on CRISPR-Cas12a</a> </h4> <p class="text-ellipsis-2">Xu Zhou, Siwen Wang, Yue Ma, Yanbing Li, Guohua Deng, Jianzhong Shi, Xiurong Wang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/8054.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A reverse transcription recombinase polymerase amplification (RT-RPA)/CRISPR method was developed for rapid and sensitive detection of avian influenza virus (AIV). The new system has the potential to be an accurate, user-friendly, and inexpensive platform for point-of-care testing applications. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">VIROLOGY JOURNAL</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33936922/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Virology </span> </div> <h4> <a href="https://www.peeref.com/works/82621968" class="text-dark hover-underline">Development of a Rapid Epstein-Barr Virus Detection System Based on Recombinase Polymerase Amplification and a Lateral Flow Assay</a> </h4> <p class="text-ellipsis-2">Yidan Sun, Danni Tang, Nan Li, Yudong Wang, Meimei Yang, Chao Shen</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/8207.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Three EBV detection systems were developed with a minimum detection limit of 103 copies, which can be applied to cell line testing. The systems are simple to operate and the results can be visualized quickly, helping to ensure cell quality and experimental safety, and may be used for EBV detection in clinical samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">VIRUSES-BASEL</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82621968/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Public, Environmental & Occupational Health </span> </div> <h4> <a href="https://www.peeref.com/works/33459792" class="text-dark hover-underline">Detecting SARS-CoV-2 BA.2, BA.4, and BA.5 Variants Utilizing a Robust RT-RPA-CRISPR/Cas12a-Based Method-China, 2023</a> </h4> <p class="text-ellipsis-2">Meihui Luo, Yang Pan, Yaqing He, A. Ruhan, Changcheng Wu, Baoying Huang, Roujian Lu, Li Zhao, Bo Peng, Fei Ye, Huijuan Wang, Yuda Chen, Zhen Li, Daitao Zhang, Wenling Wang, Wenjie Tan</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed a rapid and accurate CRISPR-based detection method that can effectively detect new variants of SARS-CoV-2, aiding in tracking the emergence of novel variants. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">CHINA CDC WEEKLY</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33459792/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/82363976" class="text-dark hover-underline">Harnessing CRISPR-Cas adaptation for RNA recording and beyond</a> </h4> <p class="text-ellipsis-2">Gyeong-Seok Oh, Seongjin An, Sungchul Kim</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1241.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article reviews the CRISPR-Cas system in the adaptive immune mechanism of prokaryotes, especially the molecular structure and function of the RT-fused Cas1-Cas2 integrase and its potential application as a directional RNA-recording tool. Outstanding questions and future research directions in this field are also emphasized. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BMB REPORTS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82363976/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Veterinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/25800841" class="text-dark hover-underline">Rapid detection of bovine rotavirus a by isothermal reverse transcription recombinase polymerase amplification assays</a> </h4> <p class="text-ellipsis-2">Yuelin Liu, Libing Liu, Jinfeng Wang, Xiaoxia Sun, Yaxin Gao, Wanzhe Yuan, Jianchang Wang, Ruiwen Li</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1276.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, two RT-RPA assays (real-time RT-RPA and LFS RT-RPA) were successfully developed for the rapid detection of bovine rotavirus A (BRVA) in cattle fecal samples. The assays showed high specificity, sensitivity, and diagnostic accuracy. The developed RT-RPA assays have great potential for use in under-equipped diagnostic laboratories and point-of-need diagnosis at quarantine stations and farms, which is important for controlling BRVA-associated diarrhea in cattle herds. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BMC VETERINARY RESEARCH</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/25800841/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_authors" class="tab-pane "> <div class="nodata my-4">No Data Available</div> </div> <div id="articles_from_journal" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83039808" class="text-dark hover-underline">Application of a newly constructed NGS panel with 45 X-linked microhaplotypes demonstrates the unique value of X-MH for kinship testing and mixture analysis</a> </h4> <p class="text-ellipsis-2">Guanju Ma, Kailiang Liu, Chaolong Lu, Qingqing Du, Mengjie Zhang, Qian Wang, Guangping Fu, Junyan Wang, Chunling Ma, Bin Cong, Shujin Li, Lihong Fu</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study screened 63 X-MHs, evaluated their performance, and calculated population parameters. The panel performed well in personal identification and paternity testing, and showed unique advantages in complex kinship and male DNA mixture analyses. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83039808/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84361787" class="text-dark hover-underline">CRISPR-Cas technology in forensic investigations: Principles, applications, and ethical considerations</a> </h4> <p class="text-ellipsis-2">Ana Filipa Sobral, Ricardo Jorge Dinis-Oliveira, Daniel Jose Barbosa</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR-Cas systems are adaptive immune systems in bacteria, and CRISPR-Cas-based technologies can be used for precise genome editing. Forensic science is used to investigate and solve legal issues, and CRISPR-Cas-based methods have potential applications in the field of forensic science, but also raise ethical concerns and potential abuse of personal genetic information. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84361787/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83532016" class="text-dark hover-underline">Dense SNP-based analyses complement forensic anthropology biogeographical ancestry assessments</a> </h4> <p class="text-ellipsis-2">Sammed N. Mandape, Bruce Budowle, Heather McKiernan, Donia Slack, Sarah Mittelman, Kristen Mittelman, David Mittelman</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Forensic anthropological examinations can estimate the ancestry of human remains, but they have limitations. Genome-wide SNP testing provides a more accurate method for ancestry estimation. The study found inconsistencies between anthropological and genomic ancestry estimates, especially for admixed populations. Further validation studies and policy improvements are crucial for improving the accuracy of ancestry estimation and reducing misinformation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83532016/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83533129" class="text-dark hover-underline">Shotgun DNA sequencing for human identification: Dynamic SNP selection and likelihood ratio calculations accounting for errors</a> </h4> <p class="text-ellipsis-2">Mikkel Meyer Andersen, Marie-Louise Kampmann, Alberte Honore Jepsen, Niels Morling, Poul Svante Eriksen, Claus Bursting, Jeppe Dyrberg Andersen</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This paper presents a statistical model for human identification using shotgun sequencing data, which takes into account the sequencing error rate and can be applied to highly degraded low-quality DNA samples. An open-source R package, wgsLR, is also introduced for implementing the method. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83533129/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84342778" class="text-dark hover-underline">SMART: STR Mixture Analysis and Resolution Tools</a> </h4> <p class="text-ellipsis-2">Xianchao Ji, Lianjiang Chi, Lan Wu Chen, Jianchao Chen, Anxin Yan, Yongjiu Li, Zheng Tu, Jian Ye, Hua Chen</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article presents SMART, a software for analyzing STR mixture profiles developed within the fully continuous model framework. SMART integrates multiple models and offers various functions, such as likelihood ratio calculation, genotype resolution, and database search. Its performance was evaluated using laboratory samples and the PROVEDIt dataset, showing high sensitivity, specificity, and precision, as well as computational efficiency. SMART is expected to be a valuable tool in forensic investigations, improving the accuracy and reliability of criminal justice outcomes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84342778/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83225861" class="text-dark hover-underline">Profiling age and body fluid DNA methylation markers using nanopore adaptive sampling</a> </h4> <p class="text-ellipsis-2">Zaka Wing-Sze Yuen, Somasundhari Shanmuganandam, Maurice Stanley, Simon Jiang, Nadine Hein, Runa Daniel, Dennis McNevin, Cameron Jack, Eduardo Eyras</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> DNA methylation plays a crucial role in physiological processes and can be used as a biomarker for body fluid identification and age prediction. Current methylation detection methods rely on various techniques and markers, requiring specialized DNA preparation and biochemical treatments. This study used nanopore adaptive sampling technology to simultaneously identify age-associated and body fluid-specific methylation markers without the need for specialized DNA preparation or biochemical treatments. The technology was consistent with whole-genome bisulfite sequencing data and identified new sites strongly correlated with age. This study lays the foundation for the development of nanopore-based methods for age prediction and body fluid identification. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83225861/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83599477" class="text-dark hover-underline">The role of cats in human DNA transfer</a> </h4> <p class="text-ellipsis-2">Heidi Monkman, Roland A. H. van Oorschot, Mariya Goray</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated whether cats are reservoirs and vectors for human DNA transfer. The results showed that human DNA was prevalent on all cats, and its distribution followed a certain pattern. In addition, short-term patting contact could lead to the transfer of human DNA to cats. These findings suggest that we should consider the role of animals in DNA reporting and collect DNA evidence from animals in cases involving interactions between animals and perpetrators. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83599477/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84866026" class="text-dark hover-underline">Uncovering genetic signatures of the Walser migration in the Alps: Patterns of diversity and differentiation</a> </h4> <p class="text-ellipsis-2">Peter Resutik, Joelle Schneider, Simon Aeschbacher, Magnus Dehli Vigeland, Mario Gysi, Corinne Moser, Chiara Barbieri, Paul Widmer, Mathias Currat, Adelgunde Kratzer, Michael Kruetzen, Cordula Haas, Natasha Arora</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated the genetic diversity and differentiation of the Walser people. By comparing samples from Walser, Walser-homeland, and non-Walser Alpine communities, as well as an idealized Swiss reference population, it was found that Walser-homeland and Walser communities showed low to moderate genetic differentiation from other communities, with more remote communities showing stronger differentiation. Additionally, the rare haplogroup W6 was identified in the Walser communities. This study contributes to understanding the genetic characteristics of the Walser people, but also highlights the need for more comprehensive research. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84866026/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84265916" class="text-dark hover-underline">Towards the identification of body fluids using RT-LAMP isothermal amplification coupled with CRISPR-Cas12a</a> </h4> <p class="text-ellipsis-2">Courtney R. H. Lynch, Olivia L. Martin, Craig Billington, Rachel Fleming</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This paper describes a new technique for the detection of body fluids that combines RT-LAMP amplification with CRISPR-mediated fluorescent detection to rapidly detect mRNA markers in body fluids at a single temperature. The technique has high sensitivity and specificity and can be used for body fluid identification in forensic screening laboratories. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84265916/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83112863" class="text-dark hover-underline">Improved individual identification in DNA mixtures of unrelated or related contributors through massively parallel sequencing</a> </h4> <p class="text-ellipsis-2">Zhiyong Liu, Enlin Wu, Ran Li, Jiajun Liu, Yu Zang, Bin Cong, Riga Wu, Bo Xie, Hongyu Sun</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study evaluated the impact of potential kinship on individual identification, including MPS performance, the influence of genetic markers on kinship and NOC inference, the probability distribution of MAC and TAC, trends in LR values, and comparisons of length- and sequence-based STR genotypes. Results showed that multiple genetic markers improved the accuracy of kinship and NOC inference, the LR value of the POI depended on the mixing ratio, and the correct kinship hypothesis yielded more conservative LR values. In addition, using sequence-based STR genotypes increased the power of individual identification and the accuracy of mixture ratio inference. The MGIEasy Signature Identification Library Prep kit demonstrated robust individual identification capabilities and is suitable for forensic DNA mixture interpretation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83112863/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83911917" class="text-dark hover-underline">A preliminary study on detecting human DNA in aquatic environments: Potential of eDNA in forensics</a> </h4> <p class="text-ellipsis-2">Marie Antony Dass, Craig D. H. Sherman, Roland A. H. van Oorschot, Dadna Hartman, Gemma Carter, Annalisa Durdle</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated the detection and degradation rates of human eDNA in saltwater and freshwater samples, as well as the recovery of STR profiles and mtDNA sequencing. Results showed that human eDNA could be detected for up to 36-84 hours in water samples, with a detection limit of 205 copies/μl. Partial STR profiles could be recovered within 24 hours, and full mtDNA profiles could be recovered from freshwater samples within 48 hours and remained detectable for up to 72 hours in saltwater. This study emphasizes the importance of considering and incorporating human eDNA analysis in forensic practice. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83911917/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84482295" class="text-dark hover-underline">Benchmarking for genotyping and imputation using degraded DNA for forensic applications across diverse populations</a> </h4> <p class="text-ellipsis-2">Elena I. Zavala, Rori V. Rohlfs, Priya Moorjani</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated computational methods for handling degraded DNA in forensic applications. By simulating sequencing data of varying qualities, the performance of common genotyping and imputation methods was tested on different SNP panels. The results showed that parameters and methods developed for ancient DNA analysis performed better in degraded DNA analysis. Additionally, using a population reference panel representative of worldwide populations improved genotyping accuracy, but the low SNP density of commonly used forensic SNP panels could impact the reliability of the analysis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84482295/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84819293" class="text-dark hover-underline">The (in)dependence of single-cell data inferences on model constructs</a> </h4> <p class="text-ellipsis-2">Catherine M. Grgicak, Klaas Slooten, Robert G. Cowell, Qhawe Bhembe, Desmond S. Lun</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Single-cell analysis is important in forensics, but different models may interpret the data differently. This study compared the weight of evidence on single-cell electropherograms among three models and found that they were mostly consistent, but there were some differences in some cases. The study also found that extreme stuttering was the main cause of the differences and proposed some interpretive adaptations to improve the situation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84819293/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/84919066" class="text-dark hover-underline">Independent evaluation of an 11-CpG panel for age estimation in blood</a> </h4> <p class="text-ellipsis-2">Mie Rath Refn, Marie-Louise Kampmann, Agnes Vyoni, Jacob Tfelt-Hansen, Erik Sorensen, Sisse Rye Ostrowski, Mette Kongstad, Anastasia Aliferi, Federica Giangasparo, Niels Morling, David Ballard, Claus Borsting, Vania Pereira</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study evaluated the 11-CpG panel for age estimation and optimized it. The optimized panel was used to type Danish blood samples, and the results showed that DNA methylation at the 11 CpG loci was significantly correlated with age. A Danish age prediction model was constructed and compared with the original model, and the results were similar, but the original model had a bias towards underestimation, while the new model did not. The assay can reasonably accurately estimate the age of a single-source donor. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84919066/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83547292" class="text-dark hover-underline">Forensic efficiency evaluation of a mtDNA whole genome sequencing system constructed with long fragment amplification strategy on DNA nanoball sequencing platform</a> </h4> <p class="text-ellipsis-2">Man Chen, Chong Chen, Ning Li, Yuerong Su, Wei Cui, Yan Huang, Meiming Cai, Bofeng Zhu</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study evaluated a novel mtDNA whole genome sequencing system using long fragment amplification strategy on the DNA nanoball sequencing platform. The system demonstrated high sequencing quality and specific mtDNA sequencing efficiencies on positive control DNA and FTA bloodstain samples. In addition, the system sequencing efficiency was also confirmed among different kinds of samples. In summary, the system showed high performance in analyzing mtDNA sequence information, and had great prospects in forensic application and maternal genetic research. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83547292/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="modal fade" id="export-citation" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"><span>×</span></button> <h4 class="modal-title">Export Citation <b class="text-primary"></b></h4> </div> <div class="modal-body"> <div class="my-3 px-4 f16"> <form action="https://www.peeref.com/works/citation/download" method="GET" target="_blank"> <div class="radio"> <label> <input type="radio" name="fileType" value="PlainText" checked> <strong>Plain Text</strong> </label> </div> <div class="radio"> <label> <input type="radio" name="fileType" value="RIS"> <strong>RIS</strong> <span>- Export format compatible with most major reference management software such as Endnote and Zotero</span> </label> </div> <div class="radio"> <label> <input type="radio" name="fileType" value="BibTeX"> <strong>BibTeX</strong> <span>- Export format compatible with LaTeX</span> </label> </div> <input type="hidden" name="articleId" value="83645405"> <div class="mt-lg-4 text-center"> <button class="citation-download-btn btn btn-primary"> Export </button> </div> </form> </div> </div> </div> </div> </div> <div class="modal fade" id="share-paper" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"><span>×</span></button> <h4 class="modal-title">Share Paper <b class="text-primary"></b></h4> </div> <div class="modal-body"> <div class="my-4"> <div class="social-share"> <a class="social-share-item email" href="mailto:?subject=Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model&body=https://www.peeref.com/works/83645405" target="_blank"> <img src="https://peeref-open.s3.amazonaws.com/images/social-share-email.svg" height="16"> </a> <a class="social-share-item linkedin" href="https://www.linkedin.com/shareArticle/?url=https://www.peeref.com/works/83645405" target="_blank"> <img src="https://peeref-open.s3.amazonaws.com/images/social-share-linkedin.svg" height="16"> </a> <a class="social-share-item twitter" href="https://twitter.com/share?url=https://www.peeref.com/works/83645405" target="_blank"> <img src="https://peeref-open.s3.amazonaws.com/images/social-share-twitter.svg" height="16"> </a> <a class="social-share-item wechat" href="javascipt:;" onclick="window.open('https://api.qrserver.com/v1/create-qr-code/?size=154x154&data=https://www.peeref.com/works/83645405', 'newwindow', 'height=300, width=300')"> <img src="https://peeref-open.s3.amazonaws.com/images/social-share-wechat.svg" height="16"> </a> <a class="social-share-item facebook" href="https://www.facebook.com/sharer/sharer.php?u=https://www.peeref.com/works/83645405" target="_blank"> <img src="https://peeref-open.s3.amazonaws.com/images/social-share-facebook.svg" height="16"> </a> </div> </div> </div> </div> </div> </div> <back-top-button></back-top-button> </main> <div class="container"> <div class="footer"> <div class="col-md-10"> <div class="d-flex flex-wrap" style="font-size: 15px;"> <a href="https://www.peeref.com/works" class="mx-3 px-2"> Papers </a> <a href="https://www.peeref.com/posters" class="mx-3 px-2"> Posters </a> <a href="https://www.peeref.com/abstracts" class="mx-3 px-2"> Abstracts </a> <a href="https://www.peeref.com/webinars" class="mx-3 px-2"> Webinars </a> <a href="https://www.peeref.com/questions" class="mx-3 px-2"> Questions </a> <a href="https://www.peeref.com/hubs" class="mx-3 px-2"> Hubs </a> <a href="https://www.peeref.com/funding" class="mx-3 px-2"> Funding </a> <a href="https://www.peeref.com/journals" class="mx-3 px-2"> Journals </a> <a href="https://www.peeref.com/connect" class="mx-3 px-2"> Connect </a> <a href="https://www.peeref.com/e-collections" class="mx-3 px-2"> Collections </a> <a href="https://www.peeref.com/reviewer" class="mx-3 px-2"> Reviewers </a> <a href="https://www.peeref.com/about" class="mx-3 px-2"> About Us </a> <a href="https://www.peeref.com/about/faq" class="mx-3 px-2"> FAQs </a> <a href="https://www.peeref.com/mobile-app" class="mx-3 px-2"> Mobile App </a> <a href="https://www.peeref.com/about/privacy" class="mx-3 px-2"> Privacy Policy </a> <a href="https://www.peeref.com/about/terms-of-use" class="mx-3 px-2"> Terms of Use </a> </div> </div> <div class="col-md-2"> <div class="d-flex justify-content-end"> <div class="sns"> <a href="https://www.facebook.com/Peeref-113216326987528" target="_blank"> <i class="ivu-icon ivu-icon-logo-facebook"></i> </a> <a href="https://twitter.com/Peeref1" target="_blank"> <i class="ivu-icon ivu-icon-logo-twitter"></i> </a> </div> </div> </div> </div> <div class="copyright"> © Peeref 2019-2025. All rights reserved. </div> </div> </div> <script src="https://peeref-open.s3.amazonaws.com/js/manifest.42bb1b6291289ba30c76.js"></script> <script src="https://peeref-open.s3.amazonaws.com/js/vendor.362294362b899c82d030.js"></script> <script src="https://peeref-open.s3.amazonaws.com/js/app.ee6e6ad3c472f6e3f93d.js"></script> <script> $(function() { $('#reading-btn').click(function() { const navHeight = $("#article-sticky-navbar").height(); const readingAreaTop = $("#collection").offset().top; const readingAreaHeight = readingAreaTop - navHeight - 30; $("html, body").animate( { scrollTop: readingAreaHeight }, 400 ); return false; }) }); </script> <script src="https://peeref-open.s3.amazonaws.com/vendor/MathJax-274/MathJax.js?config=default" async></script> <script async src="https://www.googletagmanager.com/gtag/js?id=UA-152048790-1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'UA-152048790-1'); </script> <script type="text/javascript" id="cookiebanner" src="https://peeref-open.s3.amazonaws.com/js/cookiebanner.min.js" data-position="bottom" data-cookie="_uc_" data-expires="Wed, 31 Dec 2025 00:00:00 GMT" data-font-size="15px" data-close-text="Accept" data-close-precedes="false" data-close-style="border-radius: 5px; margin: 3px 15px; padding: 3px 10px; display: inline-block; font-weight: bold; background: rgb(245, 207, 71); color: rgb(16, 75, 125);" data-linkmsg="" data-moreinfo-decoration="underline" data-moreinfo="https://www.peeref.com/about/privacy" data-bg="rgb(16, 75, 125)" data-link="rgb(255, 255, 255)" data-message="Peeref uses cookies to improve your experience. Please read our <a>Privacy Policy</a> for more details."> </script> </body> </html>