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For the full experience enable JavaScript in your browser.</div></noscript> </div> </div> <div class="row"> <div class="col-md-12 lk-body-title"> <h3 style="display: inline-block;"> Cellulose molecular weight of corn stover with cotreatment </h3> </div> </div> <div class="row content-row"> <div class="content-left"> <div name="webpart" id="webpart_4243" ><div class="panel panel-portal"> <div class="panel-heading"><h3 class="panel-title pull-left" title="Subfolders"><a name="Subfolders" class="labkey-anchor-disabled"><span class="labkey-wp-title-text">Subfolders</span></a></h3> <div class="clearfix"></div></div> <div id="WebPartView778356011" class=" panel-body"> Please log in to view the Subfolder list. <div> </div> <script type="text/javascript"> function customizeProjectWebpart4243(webpartId, pageId, index) { LABKEY.requiresScript(["Ext4","Ext4ClientApi"], function() { Ext4.onReady(function() { var config = {"containerFilter":"CurrentAndFirstChildren","labelPosition":"bottom","hideCreateButton":"false","iconSize":"large","title":"Subfolders","containerTypes":"folder"}; 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} DIV.message-overflow { display:none; } DIV.message-more { position:absolute; bottom:0; right:0 } DIV.message-less { text-align:right; } .message-more A, .message-less A, .message-more A:hover, .message-less A:hover { background-color:#e0e0e0; opacity:0.8; } /* long message collapsed */ TD.message-collapsed DIV.message-container { max-height:120px; overflow-y:hidden; } TD.message-collapsed .message-more { display:inline-block; } TD.message-collapsed .message-less { display:none; } TD.message-collapsed DIV.message-overflow { display:inline-block; -ms-filter: progid:DXImageTransform.Microsoft.gradient(gradientType=0,startColor=0,endColoStr=white); background-image: -webkit-gradient(linear,left top,left bottom,from(rgba(255, 255, 255, 0)),to(rgba(255, 255, 255, 1.0))); background-image: -moz-linear-gradient(top center,rgba(255, 255, 255, 0) 20%,rgba(255, 255, 255, 1.0) 95%); bottom: 0; filter: progid:DXImageTransform.Microsoft.gradient(gradientType=0,startColor=0,endColorStr=white); height: 50px; 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aragausk@utk.edu</p> </div> <p/> </div></div></div><div name="webpart" id="webpart_4249" ><div class="panel panel-portal"> <div class="panel-heading"><h3 class="panel-title pull-left" title="Key Words"><a name="Key Words" class="labkey-anchor-disabled"><span class="labkey-wp-title-text">Key Words</span></a></h3> <span class="dropdown dropdown-rollup pull-right"><a href="#" data-toggle="dropdown" class="dropdown-toggle fa fa-caret-down"></a><ul class="dropdown-menu dropdown-menu-right"><li><a href="/labkey/CBI/ragauskas/Public%20Data/Cellulose%20molecular%20weight%20of%20corn%20stover%20with%20cotreatment/wiki-page.view?name=KeyWords&_print=1" tabindex="0" style="">Print</a></li></ul></span><div class="clearfix"></div></div> <div id="WebPartView1121930175" class=" panel-body">  <div class="labkey-wiki"> <p>Cellulose, molecular weights, corn stover, switchgrass, cotreatment</p> </div> <p/> </div></div></div><div name="webpart" id="webpart_4248" ><div class="panel panel-portal"> <div class="panel-heading"><h3 class="panel-title pull-left" title="Description"><a name="Description" class="labkey-anchor-disabled"><span class="labkey-wp-title-text">Description</span></a></h3> <span class="dropdown dropdown-rollup pull-right"><a href="#" data-toggle="dropdown" class="dropdown-toggle fa fa-caret-down"></a><ul class="dropdown-menu dropdown-menu-right"><li><a href="/labkey/CBI/ragauskas/Public%20Data/Cellulose%20molecular%20weight%20of%20corn%20stover%20with%20cotreatment/wiki-page.view?name=Description&_print=1" tabindex="0" style="">Print</a></li></ul></span><div class="clearfix"></div></div> <div id="WebPartView751273404" class=" panel-body">  <div class="labkey-wiki"> <p>Here we present a dataset of cellulose molecular weights from biomass including corn stover (CS) and switchgrass (SWG) after a cotreatment process. Two types of corn stover labeled as GLBRC-CS and POET-CS were used. Two types of switchgrass were also used labeled as wild-type and COMT switchgrass. The biomass sample was subjected to a cotreatment process consisting of mechanical disruption and consolidated bioprocessing (CBP) process using the bacterium C. thermocellum. The cotreatment for POET-CS was performed as replicated. Cellulose samples were isolated from control, no cotreatment, and cotreated biomass. Cellulose was then derivatized using anhydrous pyridine and phenyl isocyanate over 48 h at 70 ºC. The reaction was quenched by anhydrous methanol. Methanol and water mixture (7:3, v/v) was added dropwise to promote precipitation of the cellulose derivative. The solids were collected by filtration and thoroughly washed with the methanol and water mixture followed by water. The cellulose derivative was dried overnight under vacuum at 40°C. The obtained cellulose tricarbanilates was then dissolved in tetrahydrofuran (THF) at 1.0 mg/mL and the solution was filtered through a 0.45 µm PTFE filter and placed in an auto-sampler vial. The molecular weight of cellulose was measured by Agilent GPC SECurity 1200 system equipped with four Waters Styragel columns (i.e., HR0.5, HR2, HR4, and HR6) and a UV detector. THF was used as the mobile phase. Data collection and processing was performed by Polymer Standards Service WinGPC Unity software (Build 6807). The molecular weight was calculated relative to the polystyrene calibration curve. The data on molecular weights provided information about the effects of cotreatment on corn stover and switchgrass cellulose molecular changes.</p> <p> </p> </div> <p/> </div></div></div><div name="webpart" id="webpart_4274" ><div class="panel panel-portal"> <div class="panel-heading"><h3 class="panel-title pull-left" title="Data Table"><a name="Data Table" class="labkey-anchor-disabled"><span class="labkey-wp-title-text">Data Table</span></a></h3> <span class="dropdown dropdown-rollup pull-right"><a href="#" data-toggle="dropdown" class="dropdown-toggle fa fa-caret-down"></a><ul class="dropdown-menu dropdown-menu-right"><li><a href="/labkey/CBI/ragauskas/Public%20Data/Cellulose%20molecular%20weight%20of%20corn%20stover%20with%20cotreatment/wiki-page.view?name=DataTable&_print=1" tabindex="0" style="">Print</a></li></ul></span><div class="clearfix"></div></div> <div id="WebPartView499463408" class=" panel-body">  <div class="labkey-wiki"> <table cellpadding="0" cellspacing="0" border="1" style="width: 588px;"> <tbody> <tr> <td width="97"> <p> <strong>Sample label</strong> </p> </td><td width="136"> <p> <strong>Cotreatment</strong> </p> </td><td width="79"> <p> <strong>Mw</strong> </p> </td><td width="79"> <p> <strong>Mn</strong> </p> </td><td width="66"> <p> <strong>DPw</strong> </p> </td><td width="66"> <p> <strong>DPn</strong> </p> </td><td width="66"> <p> <strong>PDI</strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>GLBRC-CS</p> </td><td width="136" valign="top"> <p>Raw  control</p> </td><td width="79" valign="top"> <p>1.71E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.85E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>3294</p> </td><td width="66" valign="top"> <p>549</p> </td><td width="66" valign="top"> <p>6.0<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>GLBRC-CS</p> </td><td width="136" valign="top"> <p>No cotreatment, 60 g/L loading</p> </td><td width="79" valign="top"> <p>2.45E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.92E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>4725</p> </td><td width="66" valign="top"> <p>563</p> </td><td width="66" valign="top"> <p>8.4<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>GLBRC-CS</p> </td><td width="136" valign="top"> <p>Cotreatment, 60 g/L loading</p> </td><td width="79" valign="top"> <p>2.09E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.31E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>4028</p> </td><td width="66" valign="top"> <p>445</p> </td><td width="66" valign="top"> <p>9.1<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>POET-CS</p> </td><td width="136" valign="top"> <p>Raw feedstock</p> </td><td width="79" valign="top"> <p>1.61E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.78E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>3096</p> </td><td width="66" valign="top"> <p>535</p> </td><td width="66" valign="top"> <p>5.8<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>POET-CS</p> </td><td width="136" valign="top"> <p>No cotreatment, 60 g/L</p> </td><td width="79" valign="top"> <p>1.47E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.11E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>2832</p> </td><td width="66" valign="top"> <p>406</p> </td><td width="66" valign="top"> <p>7.0<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>POET-CS</p> </td><td width="136" valign="top"> <p>Cotreatment, 60 g/L</p> </td><td width="79" valign="top"> <p>1.18E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>1.75E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>2275</p> </td><td width="66" valign="top"> <p>337</p> </td><td width="66" valign="top"> <p>6.7<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>POET-CS</p> </td><td width="136" valign="top"> <p>Cotreatment, 60 g/L</p> </td><td width="79" valign="top"> <p>1.13E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>1.80E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>2180</p> </td><td width="66" valign="top"> <p>348</p> </td><td width="66" valign="top"> <p>6.3<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>Wild-type Switchgrass</p> </td><td width="136" valign="top"> <p>Raw  control</p> </td><td width="79" valign="top"> <p>2.18E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>3.40E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>4205</p> </td><td width="66" valign="top"> <p>655</p> </td><td width="66" valign="top"> <p>6.4<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>Wild-type Switchgrass</p> </td><td width="136" valign="top"> <p>No cotreatment, 60 g/L loading</p> </td><td width="79" valign="top"> <p>3.21E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.96E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>6190</p> </td><td width="66" valign="top"> <p>571</p> </td><td width="66" valign="top"> <p>10.8<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>Wild-type Switchgrass</p> </td><td width="136" valign="top"> <p>Cotreatment, 60 g/L loading</p> </td><td width="79" valign="top"> <p>2.15E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.05E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>4139</p> </td><td width="66" valign="top"> <p>395</p> </td><td width="66" valign="top"> <p>10.5<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>COMT Switchgrass</p> </td><td width="136" valign="top"> <p>Raw feedstock</p> </td><td width="79" valign="top"> <p>2.83E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>3.56E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>5445</p> </td><td width="66" valign="top"> <p>686</p> </td><td width="66" valign="top"> <p>7.9<strong></strong> </p> </td> </tr> <tr> <td width="97" valign="top"> <p>COMT Switchgrass</p> </td><td width="136" valign="top"> <p>Cotreatment,</p> <p>60 g/L loading</p> </td><td width="79" valign="top"> <p>2.72E+06<strong></strong> </p> </td><td width="79" valign="top"> <p>2.44E+05<strong></strong> </p> </td><td width="66" valign="top"> <p>5234</p> </td><td width="66" valign="top"> <p>470</p> </td><td width="66" valign="top"> <p>11.1<strong></strong> </p> </td> </tr> </tbody> </table> <p>Mn: number average molecular weights; Mw: weight-average molecular weights; DPw: degree of polymerization in weight-average; DPn: degree of polymerization in number-average; PDI: polydispersity index.</p> </div> <p/> </div></div></div> </div> <div class="content-right"> <div class="content-right-spacer"></div> <div name="webpart" id="webpart_4245" ><div class="panel panel-portal"> <div class="panel-heading"><h3 class="panel-title pull-left" title="Pages"><a name="Pages" 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