Synthetic Biology and Engineering - Journal

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src="/uploads/2024/05/28/171687534826sk.jpg" alt="Synthetic Biology and Engineering-logo"> </a> </div> </div> <div class="flex-grow-1 pl-5 pb-2"> <div class="d-flex justify-content-center"> <div class="left-title"> <h1 class="d-flex justify-content-between align-items-center"> Synthetic Biology and Engineering <a class="orange-color mb-0" href="/journals/sbe/apc"> <img src="/style/image/open_access.png"> Open Access </a> </h1> <div class="d-flex"> <div class="flex-grow-1"> <div class="right-title d-flex align-items-center"> <p class="text-right mr-2">ISSN: 2958-9053 <span>(Online)</span></p> <p class="text-right mr-2">2958-9045 <span>(Print)</span></p> <p class="text-right mr-2"></p> </div> <div class="item-text"> <p><strong>An Official Journal of <a href="https://synbio-zeng.lab.westlake.edu.cn/English/Home.htm">Center of Synthetic Biology and Integrated Bioengineering, Westlake University</a></strong></p> <strong><em>Synthetic Biology and Engineering</em></strong> (<strong>SBE</strong>) is an international, peer-reviewed, open access journal dealing with interdisciplinary research of synthetic biology, from living systems to industry translation, published quarterly online by SCIEPublish. </div> </div> <div class="code-img"> <img src="/uploads/2024/05/22/1716354774gg3j.png"> </div> </div> </div> </div> </div> </div> </div> </section> <section class="mb-3 book-column"> <div class="my-body-container padding0"> <div class="book-item-fixed default-hide pt-2 pb-2"> <div class="d-flex align-items-center"> <div class="left-logo mr-3"> <a href="/" alt="Back to the homepage"> <svg xmlns="http://www.w3.org/2000/svg" class="navbar-logo" xml:space="preserve" version="1.0" viewBox="0 0 5.08 1.933"> <path d="M1.021 1.245a.29.29 0 0 1-.211-.054l-.027-.023-.003-.003.056-.066.003.004a.3.3 0 0 0 .043.033.2.2 0 0 0 .128.027l.024-.007.019-.01a.07.07 0 0 0 .022-.032.1.1 0 0 0 0-.036l-.004-.014a.1.1 0 0 0-.016-.02.1.1 0 0 0-.027-.017L.994 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6a.5.5 0 0 1-.708-.708L9.293 8 3.646 2.354a.5.5 0 0 1 0-.708z"></path> <path fill-rule="evenodd" d="M7.646 1.646a.5.5 0 0 1 .708 0l6 6a.5.5 0 0 1 0 .708l-6 6a.5.5 0 0 1-.708-.708L13.293 8 7.646 2.354a.5.5 0 0 1 0-.708z"></path> </svg> </a> </div> </div> </div> </section> <section class="mb-1 avatar-news-item"> <div class="my-body-container"> <div class="section-heading border-top-0"> <h3 class="section-title"> Editors-in-Chief </h3> </div> <ul class="row mt-3"> <li class="col pb-2 mb-2"> <div class="d-flex align-items-center height100"> <div class="avatar-container"> <a class="avatar-img" href="/journals/sbe/editors"> <img src="/uploads/2022/10/20/16662469113w4w.jpg" class="avatar img-thumbnail"> </a> </div> <div class="flex-grow-1 ml-3"> <h5>Prof. Dr. An-Ping Zeng</h5> <p>Center of Synthetic Biology and Integrated Bioengineering, School of Engineering, Westlake University, Hangzhou, 310024, China</p> </div> </div> </li> <li class="col pb-2 mb-2"> <div class="d-flex align-items-center height100"> <div class="avatar-container"> <a class="avatar-img" href="/journals/sbe/editors"> <img src="/uploads/2022/10/10/1665390067sbe2.jpg" class="avatar img-thumbnail"> </a> </div> <div class="flex-grow-1 ml-3"> <h5>Prof. Dr. Shang-Tian Yang</h5> <p>Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA</p> </div> </div> </li> </ul> </div> </section> <section class="articles-list1 mb-3"> <div class="my-body-container padding0"> <div class="section-heading"> <h3 class="section-title"> Articles <span>(36)</span> <a class="right" href="/journals/sbe/articles" target="_blank"> All articles <svg xmlns="http://www.w3.org/2000/svg" width="12" height="12" fill="currentColor" class="bi bi-chevron-double-right" viewBox="0 0 16 16"> <path fill-rule="evenodd" d="M3.646 1.646a.5.5 0 0 1 .708 0l6 6a.5.5 0 0 1 0 .708l-6 6a.5.5 0 0 1-.708-.708L9.293 8 3.646 2.354a.5.5 0 0 1 0-.708z"/> <path fill-rule="evenodd" d="M7.646 1.646a.5.5 0 0 1 .708 0l6 6a.5.5 0 0 1 0 .708l-6 6a.5.5 0 0 1-.708-.708L13.293 8 7.646 2.354a.5.5 0 0 1 0-.708z"/> </svg> </a> </h3> </div> <ul class="nav nav-tabs mt-2"> <li class="nav-item"><a class="nav-link active" id="all-tab" data-toggle="tab" href="#id-all">Latest published</a></li> <li class="nav-item"><a class="nav-link" id="downloaded-tab" data-toggle="tab" href="#id-downloaded">Most downloaded</a></li> <li class="nav-item"><a class="nav-link" id="popular-tab" data-toggle="tab" href="#id-popular" role="tab">Most popular</a></li> </ul> <div class="tab-content" id="myTabContent"> <div class="tab-pane fade show active" id="id-all"> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>14 November 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/334">Sortase A-Mediated Enzyme Assembly on Multimeric Protein for Improving Mevalonate Production</a> </h3> <p class="article-abseract clamp">Microorganisms have been extensively studied for their production of valuable chemicals. However, conventional gene fusion approaches often lack versatility and can result in enzyme inactivation. This study explored an alternative strategy for inducing metabolic channeling through sortase A-mediated ligation of metabolic enzymes. Sortase A recognizes specific amino acid sequences and selectively conjugates proteins at these sites. We focused on mevalonate production as a proof-of-concept to enhance the yield by assembling metabolic enzymes on a protein scaffold using sortase A. Although metabolic enzyme complexes were successfully formed using streptavidin as a scaffold, production did not improve. The use of CutA as a scaffold led to a 1.32-fold increase in production compared with that of the strain without the scaffold, demonstrating the efficacy of CutA in mevalonate production. These findings suggest that using sortase A to assemble metabolic enzymes onto a scaffold can effectively enhance microbial bioproduction.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=MunenoriHashimoto" target="_blank"> Munenori Hashimoto </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=MasahiroFujitani" target="_blank"> Masahiro Fujitani </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=TakuyaMatsumoto" target="_blank"> Takuya Matsumoto* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=RyosukeYamada" target="_blank"> Ryosuke Yamada </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=HiroyasuOgino" target="_blank"> Hiroyasu Ogino </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202411/14/b89538a7b3b1d1981a24c21ed9a6f4de.png" data-lightbox="image-1" data-title=""><img src="/uploads/image/202411/14/b89538a7b3b1d1981a24c21ed9a6f4de.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>13 November 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/332">Recent Advances in Developing Aldehyde-Accumulating Microbes and Future Perspective of Biosynthetic Applications</a> </h3> <p class="article-abseract clamp">Aldehydes are a class of compounds that contain carbonyl groups in their side chains and are widely used in industries such as fragrances, flavoring compounds, and pharmaceutical intermediates. In recent years, there has been a substantial rise in the application of microbial synthesis to generate aldehyde compounds and their derivatives. This review will conduct an in-depth analysis of the literature related to the manipulation of microorganisms for aldehyde accumulation and the subsequent generation of aldehyde-derived products using metabolic engineering and synthetic biology principles. Furthermore, the review further highlights the prospects and future potential of employing these aldehyde-accumulating microorganisms to synthesize a diverse range of value-added chemicals.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=YueyangChen" target="_blank"> Yueyang Chen </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=JianFan" target="_blank"> Jian Fan </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=QiwenMo" target="_blank"> Qiwen Mo </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=YongmingChoe" target="_blank"> Yongming Choe </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=HaofengChen" target="_blank"> Haofeng Chen </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=YangZhang" target="_blank"> Yang Zhang </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=ZheyongXue" target="_blank"> Zheyong Xue* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=JifengYuan" target="_blank"> Jifeng Yuan* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/11/14/fbafa73128b08cbab3ccd9faf7564226.png" data-lightbox="image-2" data-title=""><img src="/uploads/2024/11/14/fbafa73128b08cbab3ccd9faf7564226.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>30 September 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/294">Synthetic Biology in Nigeria: The Level of Awareness amongst Stakeholders</a> </h3> <p class="article-abseract clamp">Synthetic biology, an emerging field at the intersection of biotechnology and engineering, has seen a global surge in application and awareness, necessitating a comprehensive understanding of its safe potentials to drive the bio-economy. This study aimed to assess the awareness and perceptions of synthetic biology among Nigerian biosciences stakeholders, including researchers, academicians, policymakers and students. The study employed a purposive online survey targeting diverse bioscience individuals and groups across Nigeria’s six geopolitical zones. The study received 107 responses from balanced gender representation with majority within the age group of 31<b>–</b>45 years old. The findings revealed a significant knowledge gap, with only 27.1% of respondents familiar with synthetic biology and 23.4% entirely unaware of it. Most respondents associated synthetic biology with biotechnology or genetic engineering and identified its applications to be in agriculture, medicine, environmental sustainability and research. Despite recognizing its benefits, many expressed concerns about safety, ethics, and regulation; notably, 43.9% of the respondents had concerns about synthetic biology with primary focus on safety and ethical implications. As regards the regulation of synthetic biology, the study showed that 80.4% of the respondents supported the need for synthetic biology regulation with few of the respondents (16.8%) aware of existing agency mandated to regulate synthetic biology. The respondents provided valuable insights into the various ways synthetic biology can be advanced in Nigeria which include increased awareness and capacity building, engagement through social media platforms, integration into education curricula and increased funding and investment in the research. The overall sentiment towards synthetic biology was positive, with 81.3% supporting its practice and 76.6% recognizing its positive global impact. However, a significant portion of respondents remained undecided. This study concludes that there is substantial gap in the knowledge of synthetic biology among bioscience stakeholders in Nigeria and the need for a heightened advocacy including continuous conferences and symposiums for the Nigeria bioscience community on the global potentials, concerns and regulation of synthetic biology. This will foster the acceptance of safe and responsible synthetic biology in Nigeria, thereby contributing to the broader national bio-economy development.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Jude ChukwuemekeIgborgbor" target="_blank"> Jude Chukwuemeke Igborgbor* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=OnyekaKingsleyNwosu" target="_blank"> Onyeka Kingsley Nwosu </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=AbubakarMadika" target="_blank"> Abubakar Madika </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=GeoffreyOtim" target="_blank"> Geoffrey Otim </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=EmmanuelAdamolekun" target="_blank"> Emmanuel Adamolekun </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/09/30/cb9583ca983d779761720392979f378e.png" data-lightbox="image-3" data-title=""><img src="/uploads/2024/09/30/cb9583ca983d779761720392979f378e.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>26 August 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/258">Delivery of Novel Replicating Vectors to <i>Synechococcus </i>sp.<i> </i>PCC 7002 Via Natural Transformation of Plasmid Multimers</a> </h3> <p class="article-abseract clamp">In most cyanobacteria, genetic engineering efforts currently rely upon chromosomal integration; a time-consuming process due to their polyploid nature. To enhance strain construction, here we develop and characterize two novel replicating plasmids for use in <i>Synechococcus </i>sp. PCC 7002. Following an initial screen of plasmids comprising seven different origins of replication, two were found capable of replication: one based on the WVO1 broad host range plasmid and the other a shuttle vector derived from pCB2.4 from <i>Synechocystis</i> sp. PCC 6803. These were then used to construct a set of new replicating plasmids, which were shown to be both co-transformable and stably maintained in PCC 7002 at copy numbers between 7<b>–</b>16 and 0.6<b>–</b>1.4, respectively. Lastly, we demonstrate the importance of using multimeric plasmids during natural transformation of PCC 7002, with higher order multimers providing a 30-fold increase in transformation efficiency relative to monomeric plasmids. Useful considerations and methods for enhancing multimer content in plasmid samples are also presented.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=CodyKamoku" target="_blank"> Cody Kamoku </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=CheyannaCooper" target="_blank"> Cheyanna Cooper </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=AshleyStraub" target="_blank"> Ashley Straub </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=NathanMiller" target="_blank"> Nathan Miller </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=DavidR.Nielsen" target="_blank"> David R. Nielsen* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/08/26/376bb87ae097c4bfacb697813ef8208f.png" data-lightbox="image-4" data-title=""><img src="/uploads/2024/08/26/376bb87ae097c4bfacb697813ef8208f.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>22 August 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/255">Current Application of Modeling and Cell-Free System for Synthetic Gene Circuit Design</a> </h3> <p class="article-abseract clamp">The desire to harness nature’s capability of precise gene expression regulation has motivated the pursuit of synthetic gene circuits. However, designing and building novel synthetic gene circuits with predictable dynamics is nontrivial. To facilitate the design, cell-free systems have emerged as an effective alternative testbed to living biological systems in characterizing and prototyping synthetic gene regulatory networks, given its relative simplicity and designability in terms of cellular contents. Meanwhile, as parameterizing and analyzing first principle-based models can shed light on the required kinetic parameter values, thus the specific regulatory components, for the desired dynamics, coupling mathematical modeling with cell-free experiments has become an effective approach in exploring novel synthetic gene circuits. In this mini-review, we provide an overview of current progress on using deterministic first principle-based mathematical modeling in conjunction with cell-free systems, in designing and characterizing novel gene circuits, as well as the standing challenges and issues with this approach.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Thales R.Spartalis" target="_blank"> Thales R. Spartalis </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=AndresLizano" target="_blank"> Andres Lizano </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Caroline E.Copeland" target="_blank"> Caroline E. Copeland </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Yong-ChanKwon" target="_blank"> Yong-Chan Kwon* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=XunTang" target="_blank"> Xun Tang* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/08/22/9bf36cbc67f22ed3b8daf549d3692342.png" data-lightbox="image-5" data-title=""><img src="/uploads/2024/08/22/9bf36cbc67f22ed3b8daf549d3692342.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>14 August 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/248">Application of Synthetic Biology to the Biosynthesis of Polyketides</a> </h3> <p class="article-abseract clamp">Polyketides (PKs) are a large class of secondary metabolites produced by microorganisms and plants, characterized by highly diverse structures and broad biological activities. They have wide market and application prospects in medicine, agriculture, and the food industry. The complex chemical structures and multiple steps of natural polyketides result in yield that cannot be met by purely synthetic methods. With the development of synthetic biology, a number of novel technologies and synthetic strategies have been developed for the efficient synthesis of polyketides. This paper first introduces polyketides from different sources and classifications, then the reconstruction of biosynthetic pathways is described using a “bottom-up” synthetic biology approach. Through methods such as enhancing precursors, relieving feedback inhibition, and dynamic regulation, the efficient production of polyketides is achieved. Finally, the challenges faced by polyketides research and future development directions are discussed.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=XiaChen" target="_blank"> Xia Chen </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=XinyingLi" target="_blank"> Xinying Li </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=GenlinZhang" target="_blank"> Genlin Zhang* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=ChaoWang" target="_blank"> Chao Wang* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=ChunLi" target="_blank"> Chun Li* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/08/14/38b8252194b97bafda3577a00d408075.png" data-lightbox="image-6" data-title=""><img src="/uploads/2024/08/14/38b8252194b97bafda3577a00d408075.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>17 June 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/215">Cytosine Deaminase-Assisted Mutator for Genome Evolution in <i>Cupriavidus necator</i></a> </h3> <p class="article-abseract clamp"><i>Cupriavidus necator</i> H16 has been intensively explored for its potential as a versatile microbial cell factory, especially for its CO<sub>2</sub> fixation capability over the past few decades. However, rational metabolic engineering remains challenging in the construction of microbial cell factories with complex phenotypes due to the limited understanding of its metabolic regulatory network. To overcome this obstacle, laboratory adaptive evolution emerges as an alternative. In the present study, CAM (cytosine deaminase-assisted mutator) was established for the genome evolution of <i>C. necator</i>, addressing the issue of low mutation rates. By fusing cytosine deaminase with single-stranded binding proteins, CAM introduced genome-wide C-to-T mutations during DNA replication. This innovative approach could boost mutation rates, thereby expediting laboratory adaptive evolution. The applications of CAM were demonstrated in improving cell factory robustness and substrate utilization, with H<sub>2</sub>O<sub>2</sub> resistance and ethylene glycol utilization as illustrative case studies. This genetic tool not only facilitates the development of efficient cell factories but also opens avenues for exploring the intricate phenotype-genotype relationships in <i>C. necator</i>.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=HaojiePan" target="_blank"> Haojie Pan </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=ZhijiaoWang" target="_blank"> Zhijiao Wang </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=JiazhangLian" target="_blank"> Jiazhang Lian* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/06/17/faccf6ae395b9d06de3fddda3f161c96.jpg" data-lightbox="image-7" data-title=""><img src="/uploads/2024/06/17/faccf6ae395b9d06de3fddda3f161c96.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>24 May 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/192">Current Progress on Microbial <sc>l</sc>-malic Acid Production</a> </h3> <p class="article-abseract clamp">As an important intermediate in the tricarboxylic acid (TCA) cycle, <sc>l</sc>-malic acid (<sc>l</sc>-MA) is also one of the 12 important platform bulk chemicals with high added value. Owing to its various applications in food, pharmaceuticals, cosmetics and industry, the global <sc>l</sc>-MA market size is growing year by year. Over the last few decades, increasing concerns regarding fossil fuels depletion and excessive CO<sub>2</sub> emissions have led the global commitment to fostering a green economy and sustainable development. Alternatively, the sustainable microbial fermentation of <sc>l</sc>-MA has gradually attracted more and more attention. Here, this review summarizes the common <sc>l</sc>-MA biosynthesis pathways and compares the differences between different chassis microorganisms as well. Moreover, regulation strategies of genetic metabolic engineering and fermentation process to boost the <sc>l</sc>-MA production are summarized, and the research status of <sc>l</sc>-MA production from the cheaper substrates is also discussed. Finally, the direction of further exploration of industrialized <sc>l</sc>-MA biosynthesis is proposed, which provides a theoretical guidance on promoting technological innovation in industrial <sc>l</sc>-MA production.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=LuMou" target="_blank"> Lu Mou </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=MinQiu" target="_blank"> Min Qiu </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=WankuiJiang" target="_blank"> Wankui Jiang </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=WenmingZhang" target="_blank"> Wenming Zhang </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=FengxueXin" target="_blank"> Fengxue Xin </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=YujiaJiang" target="_blank"> Yujia Jiang* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=MinJiang" target="_blank"> Min Jiang* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2024/05/24/0a0ce41575802a5cc06df312c5616cd7.jpg" data-lightbox="image-8" data-title=""><img src="/uploads/2024/05/24/0a0ce41575802a5cc06df312c5616cd7.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>20 May 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/188"><p class="12title"> Advancements in the Bio-degradation of Plastic Waste into Value-added Chemicals: A Recent Perspective </p></a> </h3> <p class="article-abseract clamp">Plastics are an essential component of modern life, but the plastic waste has caused significant environmental pollution and economic losses. The effective solution to these problems is the biodegradation and high-value conversion of plastic waste. After biodegradation, plastic waste is broken into smaller molecules and eventually transformed into innocuous substances like water, carbon dioxide and biomass. High-value conversion enables plastic waste to be converted into products with higher economic value and environmental friendliness. Based on this, we summarize the biodegradation methods of bioplastics and analyze the shortage of these methods. Subsequently, we summarize the progress of converting the degradation products into value-added chemicals, comprehensively analyze the advantages and disadvantages of these bioconversion process, and propose some strategies to address these disadvantages. Finally, we analyze the significance of establishing a microbial-based conversion process that integrates the degradation and the conversion, and propose some potential strategies.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=MingdaLi" target="_blank"> Mingda Li </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=ZhenyaChen" target="_blank"> Zhenya Chen* </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Yi-XinHuo" target="_blank"> Yi-Xin Huo </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202405/20/f2d059e30da0b748348856e5a0d6285e.jpg" data-lightbox="image-9" data-title=""><img src="/uploads/image/202405/20/f2d059e30da0b748348856e5a0d6285e.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>13 May 2024</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/184">Expression of Redox Partner Fusions for Light Driven Cytochrome P450s in the Cyanobacterium <i>Synechocystis </i>sp.<i> </i>PCC. 6803</a> </h3> <p class="article-abseract clamp">Cytochrome P450s (P450s) catalyze stereo- and regioselective monooxygenations in the biosynthesis of a wide range of valuable natural compounds. The turnover of P450s requires dedicated electron transfer, usually via a NADPH-dependent reductase. The need for an NADPH-dependent reductase can be circumvented if expressed in photosynthetic organisms by exploiting the photosynthetic reducing power. However, partitioning reducing equivalents towards the P450s needs further optimization. Using our model P450, SbCYP79A1, we have previously shown that by targeting this P450 to the thylakoid membrane, the P450 can obtain its reducing power directly from photosystem I via soluble ferredoxin. Furthermore, we demonstrated using transient expression that fusing a soluble electron carrier to this P450 improves electron partitioning towards the P450 in tobacco. In order to characterize these fusions in a stably transformed organism, we expressed three different redox partner fusions in the cyanobacterium <em>Synechocystis</em> sp. PCC. 6803. We show that biochemical trends observed in the tobacco system are recapitulated in stably transformed <em>Synechocystis</em> sp. PCC. 6803. Overall, the FMN binding domain fusion produces the most oxime per unit of enzyme with and without the presence of the endogenous competing electron sink FNR and NADP<sup>+</sup>. However, the overall yield of oxime is comparable to the other strains, due to poor steady state levels of the fusion protein. <em>Synechocystis</em> sp. PCC. strains expressing the P450-FMN fusion also display a chlorotic phenotype that can be rescued by switching the nitrogen source from nitrate to ammonia, implying impaired nitrate assimilation. Optimizing electron transport towards the P450 is indeed possible <em>in vivo</em> but also highlights interference with native metabolic processes.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=LawrenceSutardja" target="_blank"> Lawrence Sutardja </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Silas BusckMellor" target="_blank"> Silas Busck Mellor </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=NadiaDodge" target="_blank"> Nadia Dodge </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=AnnemarieMatthes" target="_blank"> Annemarie Matthes </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=MeikeBurow" target="_blank"> Meike Burow </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=AgnieszkaZygadloNielsen" target="_blank"> Agnieszka Zygadlo Nielsen </a> </div> <div class="author-name"> <a href="https://scholar.google.com/scholar?q=Poul ErikJensen" target="_blank"> Poul Erik Jensen* </a> </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202405/27/f7ff61771613648e214e4cad9909af4d.png" data-lightbox="image-10" data-title=""><img src="/uploads/image/202405/27/f7ff61771613648e214e4cad9909af4d.png" class=""></a> </div> </div> </div> </div> <div class="tab-pane fade" id="id-downloaded"> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Commentary</h4> <span>14 September 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/69">Synthetic Biology Industry in China: Current State and Future Prospects</a> </h3> <p class="article-abseract clamp">In this article, we provided an overview of the current state of the SynBio industry in China with a focus on its research and technology, its main applications, and major players. We also discussed future prospects including the challenges and advantages of the SynBio industry in China.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> WeiLuo </div> <div class="author-name"> YangZhang </div> <div class="author-name"> JunPeng </div> <div class="author-name"> LishanZhao </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/09/15/e694ff0b48101afa63ebf749d34bfecb.png" data-lightbox="image-1" data-title=""><img src="/uploads/2023/09/15/e694ff0b48101afa63ebf749d34bfecb.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>31 October 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/81">Metabolic Engineering of Microorganisms Towards the Biomanufacturing of Non-Natural C5 and C6 Chemicals</a> </h3> <p class="article-abseract clamp">Five-carbon (C5) and six-carbon (C6) chemicals are essential components in the manufacturing of a variety of pharmaceuticals, fuels, polymers, and other materials. However, the predominant reliance on chemical synthesis methods and unsustainable feedstock sources has placed significant strain on Earth’s finite fossil resources and the environment. To address this challenge and promote sustainability, significant efforts have been undertaken to re-program microorganisms through metabolic engineering and synthetic biology approaches allowing for bio-based manufacturing of these compounds. This review provides a comprehensive overview of the advancements in microbial production of commercially significant non-natural C5 chemicals, including 1-pentanol, 1,5-pentanediol, cadaverine, δ-valerolactam, glutaric acid, glutaconic acid, and 5-hydroxyvaleric acid, as well as C6 chemicals, including<em> cis</em>, <em>cis</em>-muconic acid, adipic acid, 1,6-hexamethylenediamine, 6-aminocaproic acid, β-methyl-δ-valerolactone, 1-hexanol, ε-caprolactone, 6-hydroxyhexanoic acid, and 1,6-hexanediol.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Ashley Tseng </div> <div class="author-name"> VannaNguyen </div> <div class="author-name"> YuhengLin </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202310/31/540afeee76f09f635eaf5b37063bb913.png" data-lightbox="image-2" data-title=""><img src="/uploads/image/202310/31/540afeee76f09f635eaf5b37063bb913.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Perspective</h4> <span>29 December 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/103">A Perspective in Future Biomanufacturing: Challenges in Industrial Fermentation—Understanding and Controlling Microbial Lifespan and Aging</a> </h3> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Shang-Tian Yang </div> <div class="author-name"> Geng Wang </div> <div class="author-name"> Zhen Qin </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202312/29/e81be6a85cba077477f6e2131d65d69b.png" data-lightbox="image-3" data-title=""><img src="/uploads/image/202312/29/e81be6a85cba077477f6e2131d65d69b.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>13 March 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/24">Design of Oscillatory Networks through Post-Translational Control of Network Components</a> </h3> <p class="article-abseract clamp">Many essential functions in biological systems, including cell cycle progression and circadian rhythm regulation, are governed by the periodic behaviors of specific molecules. These periodic behaviors arise from the precise arrangement of components in biomolecular networks that generate oscillatory output signals. The dynamic properties of individual components of these networks, such as maturation delays and degradation rates, often play a key role in determining the network's oscillatory behavior. In this study, we explored the post-translational modulation of network components as a means to generate genetic circuits with oscillatory behaviors and perturb the oscillation features. Specifically, we used the NanoDeg platform—A bifunctional molecule consisting of a target-specific nanobody and a degron tag—to control the degradation rates of the circuit’s components and predicted the effect of NanoDeg-mediated post-translational depletion of a key circuit component on the behavior of a series of proto-oscillating network topologies. We modeled the behavior of two main classes of oscillators, namely relaxation oscillator topologies (the activator-repressor and the Goodwin oscillator) and ring oscillator topologies (repressilators). We identified two main mechanisms by which non-oscillating networks could be induced to oscillate through post-translational modulation of network components: an increase in the separation of timescales of network components and mitigation of the leaky expression of network components. These results are in agreement with previous findings describing the effect of timescale separation and mitigation of leaky expression on oscillatory behaviors. This work thus validates the use of tools to control protein degradation rates as a strategy to modulate existing oscillatory signals and construct oscillatory networks. In addition, this study provides the design rules to implement such an approach based on the control of protein degradation rates using the NanoDeg platform, which does not require genetic manipulation of the network components and can be adapted to virtually any cellular protein. This work also establishes a framework to explore the use of tools for post-translational perturbations of biomolecular networks and generates desired behaviors of the network output.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Brianna E.K. Jayanthi </div> <div class="author-name"> Shridhar Jayanthi </div> <div class="author-name"> Laura Segatori </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/04/04/82741c75c3e6de4609eac6451153dc1c.png" data-lightbox="image-4" data-title=""><img src="/uploads/2023/04/04/82741c75c3e6de4609eac6451153dc1c.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>01 September 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/66"><em>In Vitro </em>BioTransformation (ivBT): Definitions, Opportunities, and Challenges</a> </h3> <p class="article-abseract clamp">Great needs always motivate the birth and development of new disciplines and tools. Here we propose <em>in vitro</em> BioTransformation (ivBT) as a new biomanufacturing platform, between the two dominant platforms—microbial fermentation and enzymatic biocatalysis. ivBT mediated by <em>in vitro </em>synthetic enzymatic biosystems (ivSEBs) is an emerging biomanufacturing platform for the production of biocommodities (i.e., low-value and bulk biochemicals). ivSEB is the <em>in vitro </em>reconstruction of artificial (non-natural) enzymatic pathways with numerous natural enzymes, artificial enzymes, and/or (biomimetic or natural) coenzymes without living cell’s constraints, such as cell duplication, basic metabolisms, complicated regulation, bioenergetics, and so on. The two great needs (i.e., food security and the carbon-neutral renewable energy system) have motivated the birth and development of ivBT. Food security could be addressed by making artificial food from nonfood lignocellulose and artificial photosynthesis for starch synthesis from CO2. The carbon-neutral renewable energy system could be addressed by the construction of the electricity-hydrogen-carbohydrate cycle, where starch could be a high density of hydrogen carrier (up to 14.8% H<sub>2</sub> <em>wt</em>/<em>wt</em>) and an electricity storage compound (greater than 3000 Wh/kg). Also, ivBT can make a number of biocommodities, such as inositol, healthy sweeteners (e.g., D-allulose, D-tagatose, D-mannose), advanced biofuels, polymer precursors, organic acids, and so on. The industrial biomanufacturing of the first several biocommodities (e.g., <em>myo</em>-inositol, D-tagatose, D-mannose, and cellulosic starch) would wipe off any prejudice and doubt on ivBT. Huge potential markets of biocommodities with more than tens of trillions of Chinese Yuan would motivate scientists and engineers to address the remaining technical challenges and develop new tools within the next decade.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Yi-HengP. JobZhang </div> <div class="author-name"> Zhiguang Zhu </div> <div class="author-name"> ChunYou </div> <div class="author-name"> Lingling Zhang </div> <div class="author-name"> KuanqingLiu </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/09/13/d16d3cd7dd72a022fa3a1bf6b719af95.png" data-lightbox="image-5" data-title=""><img src="/uploads/2023/09/13/d16d3cd7dd72a022fa3a1bf6b719af95.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Editorial</h4> <span>13 December 2022</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/9">A New Phase of Synthetic Biology and A New Journal for Its Twin with Engineering for Biomanufacturing</a> </h3> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> An-Ping Zeng </div> <div class="author-name"> Shang-Tian Yang </div> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>10 May 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/41">Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of <em>Escherichia coli</em></a> </h3> <p class="article-abseract clamp">1,2,4-butanetriol (BT) is a compound of high interest with applications in pharmaceutical and materials. In this work, we designed a novel biosynthetic pathway for BT from glucose via a nonessential amino acid homoserine. This non-natural pathway used an engineered phosphoserine transaminase (SerC<sub>R42W/R77W</sub>) to achieve the deamination of homoserine to 4-hydroxy-2-oxobutanoic acid (HOBA). Three consecutive enzymes including a lactate dehydrogenase, a 4-hydroxybutyrate CoA-transferase and a bifunctional aldehyde/alcohol dehydrogenase are used to catalyze HOBA to BT. To enhance the carbon flux to homoserine, a homoserine-producing <i>Escherichia coli </i>was developed by improving the overexpression of two relevant key genes <i>met</i>L and <i>lys</i>C (V339A). The simultaneous overexpression of the genes encoding these enzymes for the homoserine-derived BT pathway enabled production of 19.6 mg/L BT from glucose in the homoserine-producing <i>E. coli</i>.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Yujun Zhang </div> <div class="author-name"> Lin Chen </div> <div class="author-name"> Antu Thomas </div> <div class="author-name"> An-Ping Zeng </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202305/10/19894961cb2a3424091a4c68cb9a0a49.png" data-lightbox="image-7" data-title=""><img src="/uploads/image/202305/10/19894961cb2a3424091a4c68cb9a0a49.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>16 February 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/18">Increasing Nutritional Value of Cyanobacteria by Engineering Valine, Phenylalanine, and Fatty Acid Production</a> </h3> <p class="article-abseract clamp">In 2020, the United Nations estimated that 2.37 billion people globally were without food or unable to eat a healthy balanced diet. The number of people with insufficient nutrition has increased in the short term due to COVID-19 pandemic and longer-term climate change is leading to shifts in arable land and water availability leading to a continued need to develop scalable sources of nutrition. One of the options that can yield high food mass per square foot of land use is the high-density culture of microalgae or other photosynthetic microorganisms. While photosynthetic microorganisms may provide high amounts of biomass with a small land footprint, the nutritional value of unmodified microorganisms may be limited. This mini-review presents the base nutritional value in terms of macro- and micronutrients of several cyanobacteria (<em>Nostoc</em>, <em>Anabaena</em>, <em>Spirulina</em>) in relation to established human nutritional requirements as a starting point for better utilization of cyanobacteria as nutritional supplements. It also discusses synthetic biology approaches that have been implemented in different organisms to increase the production of L-valine, L-phenylalanine, and fatty acids demonstrating some common genetic engineering design approaches and some approaches that are organism-specific.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> NickLopez-Riveira </div> <div class="author-name"> StephenS.Fong </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/03/17/690ed8b1f098362429ff28b0bbfa2302.jpg" data-lightbox="image-8" data-title=""><img src="/uploads/2023/03/17/690ed8b1f098362429ff28b0bbfa2302.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>31 May 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/46">Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered <em>Corynebacterium glutamicum</em></a> </h3> <p class="article-abseract clamp">The implementation of bioprocesses in an economically feasible and industrial competitive manner requires the optimal allocation of resources for a balanced distribution between biomass formation and product synthesis. The decoupling of growth and production in two-stage bioprocesses, aiming to ensure sufficient growth before the onset of production, is particularly relevant when target products inhibit growth. In order to avoid expensive inducer molecules, continuing process monitoring, elaborate individual process optimization, and strain engineering, we developed and applied nitrogen deprivation-induced expression of genes for product biosynthesis. Two native nitrogen deprivation-inducible promoters were identified and shown to function for dynamic growth-decoupled gene expression or CRISPRi-mediated gene knockdown in <em>C. glutamicum</em> with superior induction factors than the standard IPTG-inducible P<em><sub>trc</sub></em> promoter. Valorization of xylose to produce either the sugar acid xylonic acid or the sugar alcohol xylitol from xylose as sole source of carbon and energy was demonstrated. Competitive titers of up to 34 g·L<sup>−1</sup> xylonate and 13 g·L<sup>−1</sup> xylitol were achieved in two-stage processes. We discussed that the transfer to bioprocesses with <em>C. glutamicum</em> using carbon sources other than xylose appears straightforward in particular regarding production of growth-inhibitory compounds by their growth-decoupled fermentative production.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> LynnS.Schwardmann </div> <div class="author-name"> Marielle Rieks </div> <div class="author-name"> Volker F.Wendisch </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202306/01/3a05930c0569dac333e98790d09abed3.jpg" data-lightbox="image-9" data-title=""><img src="/uploads/image/202306/01/3a05930c0569dac333e98790d09abed3.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>07 February 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/17">Production of Highly Modified C<sub>30</sub>-carotenoids with Singlet Oxygen-quenching Activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic Acid, and Its Three Intermediates Using Genes from <em>Planococcus maritimus</em> Strain iso-3</a> </h3> <p class="article-abseract clamp"><i>Planococcus maritimus</i> strain iso-3 was previously isolated from intertidal sediment in the North Sea and was found to produce a highly modified C<sub>30</sub>-carotenoid, methyl-5-glucosyl-5,6-dihydro-4,4’-diapolycopenoate, as the final product. In this study, we analyzed the function of the carotenoid terminal oxidase <i>crtP</i> (renamed <i>cruO</i>) and aldehyde dehydrogenase <i>aldH</i> genes in <i>P. maritimus</i> strain iso-3 and elucidated the carotenoid biosynthetic pathway for this strain at the gene level. We produced four novel C<sub>30</sub>-carotenoids with potent singlet oxygen-quenching activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic acid and its three intermediates, which were obtained using <i>E. coli</i> cells carrying the <i>cruO</i> (and <i>aldH</i>) gene(s) in addition to the known <i>P. maritimus</i> carotenogenic genes.</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> MoeHagiwara </div> <div class="author-name"> ChinatsuMaehara </div> <div class="author-name"> MihoTakemura </div> <div class="author-name"> NorihikoMisawa </div> <div class="author-name"> KazutoshiShindo </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/03/20/f2f644f9f6a345e666c17f48d3f27884.jpg" data-lightbox="image-10" data-title=""><img src="/uploads/2023/03/20/f2f644f9f6a345e666c17f48d3f27884.jpg" class=""></a> </div> </div> </div> </div> <div class="tab-pane fade" id="id-popular"> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>10 May 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/41">Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of <em>Escherichia coli</em></a> </h3> <p class="article-abseract clamp"> 1,2,4-butanetriol (BT) is a compound of high interest with applications in pharmaceutical and materials. In this work, we designed a novel biosynthetic pathway for BT from glucose via a nonessential amino acid homoserine. This non-natural pathway used an engineered phosphoserine transaminase (SerC<sub>R42W/R77W</sub>) to achieve the deamination of homoserine to 4-hydroxy-2-oxobutanoic acid (HOBA). Three consecutive enzymes including a lactate dehydrogenase, a 4-hydroxybutyrate CoA-transferase and a bifunctional aldehyde/alcohol dehydrogenase are used to catalyze HOBA to BT. To enhance the carbon flux to homoserine, a homoserine-producing <i>Escherichia coli </i>was developed by improving the overexpression of two relevant key genes <i>met</i>L and <i>lys</i>C (V339A). The simultaneous overexpression of the genes encoding these enzymes for the homoserine-derived BT pathway enabled production of 19.6 mg/L BT from glucose in the homoserine-producing <i>E. coli</i>.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Yujun Zhang </div> <div class="author-name"> Lin Chen </div> <div class="author-name"> Antu Thomas </div> <div class="author-name"> An-Ping Zeng </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202305/10/19894961cb2a3424091a4c68cb9a0a49.png" data-lightbox="image-1" data-title=""><img src="/uploads/image/202305/10/19894961cb2a3424091a4c68cb9a0a49.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>06 April 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/37">Coiled Coils as Versatile Modules for Mammalian Cell Regulation</a> </h3> <p class="article-abseract clamp"> Synthetic biology is a rapidly growing field that allows us to better understand biological processes at the molecular level, and enables therapeutic interventions and biotechnological applications. One of the most powerful tools in synthetic biology is the small, customizable, and modular protein–protein interaction domains, which is used to regulate a wide variety of processes within mammalian cells. Here we review designed coiled coil dimers that represent a set of heterodimerization domains with many advantages. These dimers have been useful for directing the localization of selected proteins within cells, enhancing chemical or light-regulated transcription, creating fast proteolysis-based responsive systems and protein secretion, genome editing, and cell–cell interaction motifs. Additionally, we will discuss how these building blocks are used in diverse applications, such as CAR T cell regulation and genome editing. Finally, we will look at the potential for future advances in synthetic biology using these building modules.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> EsteraMerljak </div> <div class="author-name"> AnjaGolob-Urbanc </div> <div class="author-name"> TjašaPlaper </div> <div class="author-name"> RomanJerala </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202304/06/af615dbcbcc0797634bcdd2bf05cabc7.png" data-lightbox="image-2" data-title=""><img src="/uploads/image/202304/06/af615dbcbcc0797634bcdd2bf05cabc7.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>15 March 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/26"><em>Thermoanaerobacter</em> Species: The Promising Candidates for Lignocellulosic Biofuel Production</a> </h3> <p class="article-abseract clamp"> <i>Thermoanaerobacter</i> species, which have broad substrate range and high operating temperature, can directly utilize lignocellulosic materials for biofuels production. Compared with the mesophilic process, thermophilic process shows greater prospects in consolidated bioprocessing (CBP) due to its relatively higher efficiency of lignocellulose degradation and lower risk of microbial contamination. Additionally, thermophilic conditions can reduce cooling costs, and further facilitate downstream product recovery. This review comprehensively summarizes the advances of <i>Thermoanaerobacter </i>species in lignocellulosic biorefinery, including their performance on substrates utilization, and genetic modification or other strategies for enhanced biofuels production. Furthermore, bottlenecks of sugar co-fermentation, metabolic engineering, and bioprocessing are also discussed.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> KaiqunDai </div> <div class="author-name"> ChunyunQu </div> <div class="author-name"> HongxinFu </div> <div class="author-name"> JufangWang </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/03/16/38350647173d6984597f3467fa715ebb.jpg" data-lightbox="image-3" data-title=""><img src="/uploads/2023/03/16/38350647173d6984597f3467fa715ebb.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Editorial</h4> <span>13 December 2022</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/9">A New Phase of Synthetic Biology and A New Journal for Its Twin with Engineering for Biomanufacturing</a> </h3> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> An-Ping Zeng </div> <div class="author-name"> Shang-Tian Yang </div> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>13 March 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/24">Design of Oscillatory Networks through Post-Translational Control of Network Components</a> </h3> <p class="article-abseract clamp"> Many essential functions in biological systems, including cell cycle progression and circadian rhythm regulation, are governed by the periodic behaviors of specific molecules. These periodic behaviors arise from the precise arrangement of components in biomolecular networks that generate oscillatory output signals. The dynamic properties of individual components of these networks, such as maturation delays and degradation rates, often play a key role in determining the network's oscillatory behavior. In this study, we explored the post-translational modulation of network components as a means to generate genetic circuits with oscillatory behaviors and perturb the oscillation features. Specifically, we used the NanoDeg platform—A bifunctional molecule consisting of a target-specific nanobody and a degron tag—to control the degradation rates of the circuit’s components and predicted the effect of NanoDeg-mediated post-translational depletion of a key circuit component on the behavior of a series of proto-oscillating network topologies. We modeled the behavior of two main classes of oscillators, namely relaxation oscillator topologies (the activator-repressor and the Goodwin oscillator) and ring oscillator topologies (repressilators). We identified two main mechanisms by which non-oscillating networks could be induced to oscillate through post-translational modulation of network components: an increase in the separation of timescales of network components and mitigation of the leaky expression of network components. These results are in agreement with previous findings describing the effect of timescale separation and mitigation of leaky expression on oscillatory behaviors. This work thus validates the use of tools to control protein degradation rates as a strategy to modulate existing oscillatory signals and construct oscillatory networks. In addition, this study provides the design rules to implement such an approach based on the control of protein degradation rates using the NanoDeg platform, which does not require genetic manipulation of the network components and can be adapted to virtually any cellular protein. This work also establishes a framework to explore the use of tools for post-translational perturbations of biomolecular networks and generates desired behaviors of the network output.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Brianna E.K. Jayanthi </div> <div class="author-name"> Shridhar Jayanthi </div> <div class="author-name"> Laura Segatori </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/04/04/82741c75c3e6de4609eac6451153dc1c.png" data-lightbox="image-5" data-title=""><img src="/uploads/2023/04/04/82741c75c3e6de4609eac6451153dc1c.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>31 October 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/81">Metabolic Engineering of Microorganisms Towards the Biomanufacturing of Non-Natural C5 and C6 Chemicals</a> </h3> <p class="article-abseract clamp"> Five-carbon (C5) and six-carbon (C6) chemicals are essential components in the manufacturing of a variety of pharmaceuticals, fuels, polymers, and other materials. However, the predominant reliance on chemical synthesis methods and unsustainable feedstock sources has placed significant strain on Earth’s finite fossil resources and the environment. To address this challenge and promote sustainability, significant efforts have been undertaken to re-program microorganisms through metabolic engineering and synthetic biology approaches allowing for bio-based manufacturing of these compounds. This review provides a comprehensive overview of the advancements in microbial production of commercially significant non-natural C5 chemicals, including 1-pentanol, 1,5-pentanediol, cadaverine, δ-valerolactam, glutaric acid, glutaconic acid, and 5-hydroxyvaleric acid, as well as C6 chemicals, including<em> cis</em>, <em>cis</em>-muconic acid, adipic acid, 1,6-hexamethylenediamine, 6-aminocaproic acid, β-methyl-δ-valerolactone, 1-hexanol, ε-caprolactone, 6-hydroxyhexanoic acid, and 1,6-hexanediol.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> Ashley Tseng </div> <div class="author-name"> VannaNguyen </div> <div class="author-name"> YuhengLin </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202310/31/540afeee76f09f635eaf5b37063bb913.png" data-lightbox="image-6" data-title=""><img src="/uploads/image/202310/31/540afeee76f09f635eaf5b37063bb913.png" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>07 February 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/17">Production of Highly Modified C<sub>30</sub>-carotenoids with Singlet Oxygen-quenching Activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic Acid, and Its Three Intermediates Using Genes from <em>Planococcus maritimus</em> Strain iso-3</a> </h3> <p class="article-abseract clamp"> <i>Planococcus maritimus</i> strain iso-3 was previously isolated from intertidal sediment in the North Sea and was found to produce a highly modified C<sub>30</sub>-carotenoid, methyl-5-glucosyl-5,6-dihydro-4,4’-diapolycopenoate, as the final product. In this study, we analyzed the function of the carotenoid terminal oxidase <i>crtP</i> (renamed <i>cruO</i>) and aldehyde dehydrogenase <i>aldH</i> genes in <i>P. maritimus</i> strain iso-3 and elucidated the carotenoid biosynthetic pathway for this strain at the gene level. We produced four novel C<sub>30</sub>-carotenoids with potent singlet oxygen-quenching activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic acid and its three intermediates, which were obtained using <i>E. coli</i> cells carrying the <i>cruO</i> (and <i>aldH</i>) gene(s) in addition to the known <i>P. maritimus</i> carotenogenic genes.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> MoeHagiwara </div> <div class="author-name"> ChinatsuMaehara </div> <div class="author-name"> MihoTakemura </div> <div class="author-name"> NorihikoMisawa </div> <div class="author-name"> KazutoshiShindo </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/03/20/f2f644f9f6a345e666c17f48d3f27884.jpg" data-lightbox="image-7" data-title=""><img src="/uploads/2023/03/20/f2f644f9f6a345e666c17f48d3f27884.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>22 May 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/45">Fed-batch Self-regulated Fermentation of Glucose to Co-produce Glycerol and 1,3-propanediol by Recombinant <em>Escherichia coli</em></a> </h3> <p class="article-abseract clamp"> As important bio-chemicals, glycerol and 1,3-propanediol (1,3-PDO) have been widely used in numerous fields, e.g., polymers, cosmetics, foods, lubricants, medicines, and so on. Bio-based 1,3-PDO is generally produced from glycerol or glucose by natural or recombinant strains, during which organic acids are always co-produced. In this work, acetic acid was also co-produced when 1,3-PDO was obtained from glucose by a recombinant strain of E. coli MG1655. Usually, a base was added to adjust the fermentation pH, resulting in the accumulation of organic salts and difficulty in the next down streaming process. Herein, a novel strategy was developed to consume the produced acetic acid by cells self in fed-batch self-regulated fermentation. The recombinant <em>E. coli</em> cells produced 48.33 g/L glycerol and 61.27 g/L 1,3-PDO with a total mass yield of 45.6% and without any other byproducts at the end of 5 fed-batch fermentations. The initial buffer pH, glucose concentration, pulse feeding sugar amount, time for a single batch fermentation and reducing acid were investigated by a series of comparative experiments. This fed-batch self-regulated fermentation has potential for the co-production of 1,3-PDO and glycerol, and will highlight the subsequent modification of recombinant <em>E. coli</em> strain by synthetic biology.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> GuiminLiu </div> <div class="author-name"> CaiFeng </div> <div class="author-name"> ZhiweiZhu </div> <div class="author-name"> YaqinSun </div> <div class="author-name"> ZhilongXiu </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202305/23/75bc1af5d3741ed37d162d7c6cbeefef.jpg" data-lightbox="image-8" data-title=""><img src="/uploads/image/202305/23/75bc1af5d3741ed37d162d7c6cbeefef.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Review</h4> <span>16 February 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/18">Increasing Nutritional Value of Cyanobacteria by Engineering Valine, Phenylalanine, and Fatty Acid Production</a> </h3> <p class="article-abseract clamp"> In 2020, the United Nations estimated that 2.37 billion people globally were without food or unable to eat a healthy balanced diet. The number of people with insufficient nutrition has increased in the short term due to COVID-19 pandemic and longer-term climate change is leading to shifts in arable land and water availability leading to a continued need to develop scalable sources of nutrition. One of the options that can yield high food mass per square foot of land use is the high-density culture of microalgae or other photosynthetic microorganisms. While photosynthetic microorganisms may provide high amounts of biomass with a small land footprint, the nutritional value of unmodified microorganisms may be limited. This mini-review presents the base nutritional value in terms of macro- and micronutrients of several cyanobacteria (<em>Nostoc</em>, <em>Anabaena</em>, <em>Spirulina</em>) in relation to established human nutritional requirements as a starting point for better utilization of cyanobacteria as nutritional supplements. It also discusses synthetic biology approaches that have been implemented in different organisms to increase the production of L-valine, L-phenylalanine, and fatty acids demonstrating some common genetic engineering design approaches and some approaches that are organism-specific.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> NickLopez-Riveira </div> <div class="author-name"> StephenS.Fong </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/2023/03/17/690ed8b1f098362429ff28b0bbfa2302.jpg" data-lightbox="image-9" data-title=""><img src="/uploads/2023/03/17/690ed8b1f098362429ff28b0bbfa2302.jpg" class=""></a> </div> </div> </div> <div class="d-flex article-box pt-3 pb-3"> <div class="sc-width-200 pr-3"> <h4>Article</h4> <span>19 July 2023</span> </div> <div class="sc-flex-value1 d-flex flex-wrap"> <div> <h3 class="article-title"> <a class="anchor" href="/article/pii/52">Hydroxybenzoic Acid Production Using Metabolically Engineered <em>Corynebacterium glutamicum</em></a> </h3> <p class="article-abseract clamp"> Hydroxybenzoic acids (HBAs), including 4-HBA, 3-HBA, and 2-HBA, are valuable platform chemicals for production of commodity materials and fine chemicals. Herein, we employed metabolic engineering techniques to enhance the production of these HBAs in <em>Corynebacterium glutamicum</em> ATCC 13032. Our approach augmented the shikimate pathway and eliminated genes associated with HBA degradation, particularly phenol 2-monooxygenase encoded by <em>cg2966</em>. Increased titers of 3-HBA and 4-HBA were achieved via selection of suitable promoters for 3-hydroxybenzoate synthase and chorismate pyruvate lyase. A tac-M1 promoter was suitable for chorismate pyruvate lyase expression and 8.3 g/L of 4-HBA production was achieved. Efficient production of 2-HBA was enabled by maintaining a balanced expression of isochorismate synthase and isochorismate pyruvate lyase. Consequently, strains KSD5-tacM1-H and KSD5-J2-PE exhibited production levels of 19.2 g/L of 3-HBA and 12.9 g/L of 2-HBA, respectively, using 1 L jar fermenter containing 80 g/L of glucose. Therefore, this engineered strain platform holds significant potential for production of other valuable products derived from chorismate.utf-8</p> </div> <div class="authors-list align-self-end"> <i class="fal fa-user"></i> <div class="author-name"> MisaDoke </div> <div class="author-name"> MayumiKishida </div> <div class="author-name"> YuukiHirata </div> <div class="author-name"> MarikoNakano </div> <div class="author-name"> MayoHorita </div> <div class="author-name"> DaisukeNonaka </div> <div class="author-name"> YutaroMori </div> <div class="author-name"> RyosukeFujiwara </div> <div class="author-name"> AkihikoKondo </div> <div class="author-name"> ShuheiNoda </div> <div class="author-name"> TsutomuTanaka </div> </div> </div> <div class="article-img"> <div class="img-thumbnail"> <a href="/uploads/image/202307/19/c0fd6dfe2dfc1ff59f7876c2bd02a4fb.png" data-lightbox="image-10" data-title=""><img src="/uploads/image/202307/19/c0fd6dfe2dfc1ff59f7876c2bd02a4fb.png" class=""></a> </div> </div> </div> </div> </div> </div> </section> <section id="recent-posts-4" class="widget widget_recent_entries news-card mb-2"> <div class="my-body-container padding0"> <div class="section-heading"> <h3 class="section-title"> News </h3> </div> <ul class="d-flex align-content-around flex-wrap news-list row row-cols-1 row-cols-xl-4 row-cols-lg-3 row-cols-md-2 row-cols-sm-1 mt-3 ml-n2 mr-n2"> <li class="col mb-3 pl-2 pr-2 pb-3 d-flex flex-wrap"> <div> <div class="img"> <a href="https://www.sciepublish.com/journals/sbe/awards/Best_Paper_Award_2026" target="_blank" rel="nofollow"><img src="/uploads/2024/09/11/17260356500f43.png" alt="Best Paper Award 2026" /></a> </div> <div class="uk-card-title"> <a class="anchor" href="https://www.sciepublish.com/journals/sbe/awards/Best_Paper_Award_2026" target="_blank" rel="nofollow">Best Paper Award 2026</a> </div> </div>  </li> <li class="col mb-3 pl-2 pr-2 pb-3 d-flex flex-wrap"> <div> <div class="img"> <a href="/news/nextgen-omics-us-2025"> <img src="/uploads/2024/04/25/1714014572qy93.jpg" alt="NextGen Omics US 2025" /> </a> </div> <div class="uk-card-title"> <a class="anchor" href="/news/nextgen-omics-us-2025">NextGen Omics US 2025</a> </div> </div> <div class="news-date align-self-end">MARCH 2025 | BOSTON, MA</div> </li> <li class="col mb-3 pl-2 pr-2 pb-3 d-flex flex-wrap"> <div> <div class="img"> <a href="/news/ecb-ibs-2024"> <img src="/uploads/2024/04/25/1714016364vqnu.jpg" alt="ECB-IBS 2024" /> </a> </div> <div class="uk-card-title"> <a class="anchor" href="/news/ecb-ibs-2024">ECB-IBS 2024</a> </div> </div> <div class="news-date align-self-end">30 June - 3 July 2024 丨 Rotterdam, The Netherlands</div> </li> <li class="col mb-3 pl-2 pr-2 pb-3 d-flex flex-wrap"> <div> <div class="img"> <a href="/news/sba3-0-international-synthetic-biology-ai-and-biosecurity-conference-in-africa"> <img src="/uploads/2024/04/25/1714022465nrt7.jpg" alt="SBA3.0 International Synthetic Biology, AI, and Biosecurity Conference in Africa" /> </a> </div> <div class="uk-card-title"> <a class="anchor" href="/news/sba3-0-international-synthetic-biology-ai-and-biosecurity-conference-in-africa">SBA3.0 International Synthetic Biology, AI, and Biosecurity Conference in Africa</a> </div> </div> <div class="news-date align-self-end">17th-19th July 2024丨Nairobi, Kenya</div> </li> <div class="white-border"></div> </ul> </div> </section> <section id="recent-posts-4" class="widget widget_recent_entries mb-3"> <div class="my-body-container padding0"> <div class="section-heading"> <h3 class="section-title"> Topic Collection </h3> </div> <div class="ts-box2 article-box pt-3 pb-3"> <div class="text articles-list"> <h3 class="article-title mb-2"> <a href="/journals/sbe/special_issues/molecular_tools">Molecular Tools in Synthetic Biology</a> </h3> <div class="post-tags mt-10"> <ul> <li class="article-abseract clamp card-text article-authors">Topic Editor: <span>Bernd Mueller-Roeber</span> <span>Daniel Schindler</span> </li> <li class="authors-list"> <a class="author-name" href="javascript:;"> <i class="fal fa-clock"></i></span> <span class="article-date">Deadline: 30 September 2024</span> </a> </li> </ul> </div> </div> </div> <div class="ts-box2 article-box pt-3 pb-3"> <div class="text articles-list"> <h3 class="article-title mb-2"> <a href="/journals/sbe/special_issues/syn_bio_chem_fuels">Synthetic Biology in the Manufacturing of Chemicals and Fuels</a> </h3> <div class="post-tags mt-10"> <ul> <li class="mb-2 article-abseract"> <p> <span class="mr-2">Topic in</span>Synthetic Biology; Genome Editing; CRISPR; Biofuels; Biochemicals; Non-conventional Microorganisms; Cell Free System; Carbon Metabolism; Metabolic Pathways; Carbon Sequestration; Environmental Sustainability </p> </li> <li class="article-abseract clamp card-text article-authors">Topic Editor: <span>Yi Wang</span> <span>Jufang Wang</span> <span>George N. 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