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viability</title> <meta name="description" content="Search results for: sperm viability"> <meta name="keywords" content="sperm viability"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="sperm viability"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 792</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: sperm viability</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">792</span> Supplementation of Fig Fruit (Ficus carica linn.) Extract in Extender on Sperm Motility and Viability of Native Chicken Semen after Cooling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20Isnaini">N. Isnaini</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Wahjuningsih"> S. Wahjuningsih</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fig fruit is the fruit of a tropical plant with content of flavanoids, vitamins A, C, and E which are antioxidants that effectively prevent and neutralize free radicals. This study was conducted to evaluate the supplementation of fig fruit extract in a physiological NaCl-based diluent on sperm motility and viability of native chicken semen after cooling. Semen was collected from 4 male mature chocks using massage method. Fresh semen evaluated for colour, pH, volume, concentration, mass motility, individual motility, life sperm and sperm abnormality. Semen was diluted with physiological NaCl-based extender supplemented with different levels of fig fruit extract (0, 10, 20 and 30 %) v/v with the ratio of 1 semen: 4 diluter. Semen used had mass motility of 2+ and motility of 70%. Immediately after dilution semen was stored in 3-5 °C and sperm motility and viability percentage were observed at 0, 12 and 24 h. The obtained data were analyze with Analysis of Variant (ANOVA) and Least Significant Difference were determined. The experiment was designed using completely random design (4 treatments and 10 replications). The results showed that the level of fig fruit extract had very significant effect (P < 0,01) on sperm motility and viability percentage in 0, 12 and 24 h of cooling. It can be concluded that the best fig fruit extract level for resulting optimal sperm motility and viability was 10%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chock" title="chock">chock</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=fig%20fruit%20extract" title=" fig fruit extract"> fig fruit extract</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm" title=" sperm"> sperm</a> </p> <a href="https://publications.waset.org/abstracts/39274/supplementation-of-fig-fruit-ficus-carica-linn-extract-in-extender-on-sperm-motility-and-viability-of-native-chicken-semen-after-cooling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39274.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">306</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">791</span> Gonadotoxic and Cytotoxic Effect of Induced Obesity via Monosodium Glutamate on Mus musculus Testis Cytoarchitecture and Sperm Parameter</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=I.%20Nur%20Hilwani">I. Nur Hilwani</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Nasibah"> R. Nasibah</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Nurdiana"> S. Nurdiana</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20J.%20Norashirene"> M. J. Norashirene</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Impaired fertility may be the result of indirect consumption of anti-fertility agents through food. Monosodium glutamate (MSG) has been widely used as food additive, flavour enhancer and included in vaccines. This study focuses in determining the gonadotoxic and cytotoxic effect of MSG on selected sperm parameters such as sperm viability, sperm membrane integrity and testes cytoarchitecture of male mice via histological examination to determine its effect on spermatogenesis. Twenty-four Mus musculus were randomly divided into 4 groups and given intraperitoneal injections (IP) daily for 14 days of different MSG concentrations at 250, 500 and 1000mg/kg MSG to body weight to induce obesity. Saline was given to control group. Mice were sacrificed and analysis revealed abnormalities in values for sperm parameters and damages to testes cytoarchitecture of male mice. The results recorded decreased viability (p<0.05) and integrity of sperm membrane (p>0.05) with degenerative structures in seminiferous tubule of testes. The results indicated various implications of MSG on male mice reproductive system which has consequences in fertility potential. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sperm%20parameter" title="sperm parameter">sperm parameter</a>, <a href="https://publications.waset.org/abstracts/search?q=testes%20histology" title=" testes histology"> testes histology</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20viability" title=" sperm viability"> sperm viability</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20membrane%20integrity" title=" sperm membrane integrity"> sperm membrane integrity</a> </p> <a href="https://publications.waset.org/abstracts/13066/gonadotoxic-and-cytotoxic-effect-of-induced-obesity-via-monosodium-glutamate-on-mus-musculus-testis-cytoarchitecture-and-sperm-parameter" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13066.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">347</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">790</span> The Effect of Lepidium Meyenii on Viability, Motility, and Sperm Morphology in Treatment of Infertility Among Adult Male Wistar Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arefeh%20Sabzipour">Arefeh Sabzipour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present work, the effect of Lepidium meyenii on viability, motility, and sperm morphology in the treatment of infertility of adult male Wistar rats was evaluated. 21 male Wistar rats were adopted, fed and brought up in the same conditions to reach the weight of 230±5 g. after that, they were randomly divided into three groups, including two experimental groups and one control group, each group consisted of 7 rates. Lepidium meyenii was extracted and pulverized. Mice in the control group were treated with distilled water, and experimental groups were gavage with alcoholic juice extracted from Lepidium meyenii once a day for 10 consecutive days. After rates were killed, the testes were isolated. Different parameters includes semen volume in mice, sperm count, sperm motility, morphology, and viability, were evaluated. The results shows that sperm motility and sperm survival indices were significantly different between groups, and sperm count and sperm morphology indices were not significantly different. Sperm motility index in intervention group 1 was equal to 77.00±2.499 and was significantly higher than the one in intervention group two (70.14±3.579, P=0.018) and control group (69.43 ±7.323, P=0.018). Sperm survival index was 91.14 ± 2.410 in intervention group 1, 79.43± 5.062 in intervention group 2, and 76.71.6.651 in the control group (P<0.001). Based on the results of the present study, Lepidium meyenii had great effect on improving sperm indices of mice, especially sperm motility index and sperm survival index. Sperm count index and sperm morphology index, although increased, were not statistically significant. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=infertility" title="infertility">infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=lepidium" title=" lepidium"> lepidium</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20morphology" title=" sperm morphology"> sperm morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20survival" title=" sperm survival"> sperm survival</a> </p> <a href="https://publications.waset.org/abstracts/166117/the-effect-of-lepidium-meyenii-on-viability-motility-and-sperm-morphology-in-treatment-of-infertility-among-adult-male-wistar-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/166117.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">789</span> The Effect of Lepidium Meyenii on Viability, Motility, and Sperm Morphology in Treatment of Infertility Among Adult Male Wistar Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arefeh%20Sabzipour">Arefeh Sabzipour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present work, the effect of Lepidium meyenii on viability, motility, and sperm morphology in the treatment of infertility of adult male Wistar rats was evaluated. 21 male Wistar rats were adopted, fed and brought up in the same conditions to reach the weight of 230±5 g. after that, they were randomly divided into three groups, including two experimental groups and one control group, each group consisted of 7 rates. Lepidium meyenii was extracted and pulverized. Mice in the control group were treated with distilled water, and experimental groups were gavage with alcoholic juice extracted from Lepidium meyenii once a day for 10 consecutive days. After rates were killed, the testes were isolated. Different parameters includes semen volume in mice, sperm count, sperm motility, morphology, and viability, were evaluated. The results shows that sperm motility and sperm survival indices were significantly different between groups, and sperm count and sperm morphology indices were not significantly different. Sperm motility index in intervention group 1 was equal to 77.00±2.499 and was significantly higher than the one in intervention group two (70.14±3.579, P=0.018) and control group (69.43 ±7.323, P=0.018). Sperm survival index was 91.14 ± 2.410 in intervention group 1, 79.43± 5.062 in intervention group 2, and 76.71.6.651 in the control group (P<0.001). Based on the results of the present study, Lepidium meyenii had great effect on improving sperm indices of mice, especially sperm motility index and sperm survival index. Sperm count index and sperm morphology index, although increased, were not statistically significant. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=infertility" title="infertility">infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=lepidium%20meyenii" title=" lepidium meyenii"> lepidium meyenii</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20morphology" title=" sperm morphology"> sperm morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20survival" title=" sperm survival"> sperm survival</a> </p> <a href="https://publications.waset.org/abstracts/166135/the-effect-of-lepidium-meyenii-on-viability-motility-and-sperm-morphology-in-treatment-of-infertility-among-adult-male-wistar-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/166135.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">80</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">788</span> The Effect of Lepidium Meyenii on Viability, Motility, and Sperm Morphology in Treatment of Infertility Among Adult Male Wistar Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arefeh%20Sabzipour">Arefeh Sabzipour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present work, the effect of Lepidium meyenii on viability, motility, and sperm morphology in the treatment of infertility of adult male Wistar rats was evaluated. 21 male Wistar rats were adopted, fed and brought up in the same conditions to reach the weight of 230±5 g. after that they were randomly divided into three groups including two experimental groups and one control group, each group consisted of 7 rates. Lepidium meyenii was extracted and pulverized. Mice in the control group were treated with distilled water and experimental groups were gavage with alcoholic juice extracted from Lepidium meyenii once a day for 10 consecutive days. After rates were killed, the testes were isolated. Different parameters includes semen volume in mice, sperm count, sperm motility, morphology, and viability were evaluated. The results shows that sperm motility and sperm survival indices were significantly different between groups and sperm count and sperm morphology indices were not significantly different. Sperm motility index in intervention group 1 was equal to 77.00±2.499 and was significantly higher than the one in intervention group two (70.14±3.579, P=0.018) and control group (69.43 ±7.323, P=0.018). Sperm survival index was 91.14 ± 2.410 in intervention group 1, 79.43± 5.062 in intervention group 2, and 76.71.6.651 in control group (P<0.001). Based on the results of the present study, Lepidium meyenii had great effect on improving sperm indices of mice, especially sperm motility index and sperm survival index. Sperm count index and sperm morphology index, although increased, were not statistically significant. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=infertility" title="infertility">infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=Lepidium%20meyenii" title=" Lepidium meyenii"> Lepidium meyenii</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20morphology" title=" sperm morphology"> sperm morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20survival" title=" sperm survival"> sperm survival</a> </p> <a href="https://publications.waset.org/abstracts/165987/the-effect-of-lepidium-meyenii-on-viability-motility-and-sperm-morphology-in-treatment-of-infertility-among-adult-male-wistar-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165987.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">94</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">787</span> Inductions of CaC₂ on Sperm Morphology and Viability of the Albino Mice (Mus musculus)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dike%20H.%20Ogbuagu">Dike H. Ogbuagu</a>, <a href="https://publications.waset.org/abstracts/search?q=Etsede%20J.%20Oritsematosan"> Etsede J. Oritsematosan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work investigated possible inductions of CaC₂, often misused by fruit vendors to stimulate artificial ripening, on mammalian sperm morphology and viability. Thirty isogenic strains of male albino mice, Mus musculus (age≈ 8weeks; weight= 32.5±2.0g) were acclimatized (ambient temperature 28.0±1.0°C) for 2 weeks and fed standard growers mash and water ad libutum. They were later exposed to graded toxicant concentrations (w/w) of 2.5000, 1.2500, 0.6250, and 0.3125% in 4 cages. A control cage was also established. After 5 weeks, 3 animals from each cage were sacrificed by cervical dislocation and the cauda epididymis excised. Sperm morphology and viability were determined by microscopic procedures. The ANOVA, means plots, Student’s t-test and variation plots were used to analyze data. The common abnormalities observed included Double Head, Pin Head, Knobbed Head, No Tail and With Hook. The higher toxicant concentrations induced significantly lower body weights [F(829.899) ˃ Fcrit(4.19)] and more abnormalities [F(26.52) ˃ Fcrit(4.00)] at P˂0.05. Sperm cells in the control setup were significantly more viable than those in the 0.625% (t=0.005) and 2.500% toxicant doses (t=0.018) at the 95% confidence limit. CaC₂ appeared to induced morphological abnormalities and reduced viability in sperm cells of M. musculus. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=artificial%20ripening" title="artificial ripening">artificial ripening</a>, <a href="https://publications.waset.org/abstracts/search?q=calcium%20carbide" title=" calcium carbide"> calcium carbide</a>, <a href="https://publications.waset.org/abstracts/search?q=fruit%20vendors" title=" fruit vendors"> fruit vendors</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20morphology" title=" sperm morphology"> sperm morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20viability" title=" sperm viability"> sperm viability</a> </p> <a href="https://publications.waset.org/abstracts/43338/inductions-of-cac2-on-sperm-morphology-and-viability-of-the-albino-mice-mus-musculus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43338.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">222</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">786</span> Effects of SNP in Semen Diluents on Motility, Viability and Lipid Peroxidation of Sperm of Bulls</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hamid%20Reza%20Khodaei">Hamid Reza Khodaei</a>, <a href="https://publications.waset.org/abstracts/search?q=Behnaz%20Mahdavi"> Behnaz Mahdavi</a>, <a href="https://publications.waset.org/abstracts/search?q=Alireza%20Banitaba"> Alireza Banitaba</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nitric oxide (NO) plays an important role in all sexual activities of animals. It is made in body from NO syntheses enzyme and L-arginin molecule. NO can make band with sulfur-iron complexes and due to production of steroid sexual hormones related to enzymes which have this complex, NO can change the activity of these enzymes. NO affects many cells including endothelial cells of veins, macrophages and mast cells. These cells are found in testis leydig cells and therefore are important source of NO in testis tissue. Minimizing damages to sperm at the time of sperm freezing and thawing is really important. The goal of this study was to determine the function of NO before freezing and its effects on quality and viability of sperms after thawing and incubation. 4 Holstein bulls were selected from the age of 4, and artificial insemination was done for 3 weeks (2 times a week). Treatments were 0, 10, 50 and 100 nm of sodium nitroprusside (SNP). Data analysis was performed by SAS98 program. Also, mean comparison was done using Duncan's multiple ranges test (P<0.05). Concentrations used were found to increase motility and viability of spermatozoa at 1, 2 and 3 hours after thawing significantly (P<0.05) but there was no significant difference at zero time. SNP levels reduced the amount of lipid peroxidation in sperm membrane, increased acrosome health and improved samples membranes especially in 50 and 100 nm treatments. According to results, adding SNP to semen diluents increases motility and viability of spermatozoa. Also, it reduces lipid peroxidation in sperm membrane and improves sperm function. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sperm%20motility" title="sperm motility">sperm motility</a>, <a href="https://publications.waset.org/abstracts/search?q=nitric%20oxide" title=" nitric oxide"> nitric oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20peroxidation" title=" lipid peroxidation"> lipid peroxidation</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatozoa" title=" spermatozoa"> spermatozoa</a> </p> <a href="https://publications.waset.org/abstracts/15112/effects-of-snp-in-semen-diluents-on-motility-viability-and-lipid-peroxidation-of-sperm-of-bulls" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15112.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">657</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">785</span> Cryoinjuries in Sperm Cells: Effect of Adaptation of Steps in Cryopreservation Protocol for Boar Semen upon Post-Thaw Sperm Quality</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aftab%20Ali">Aftab Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cryopreservation of semen is one of the key factors for a successful breeding business along with other factors. To achieve high fertility in boar, one should know about spermatozoa response to different treatments proceeds during cryopreservation. The running project is highly focused on cryopreservation and its effects on sperm quality parameters in both boar and bull semen. Semen sample from A, B, C, and D, were subjected to different thawing conditions and were analyzed upon different treatments in the study. Parameters like sperm cell motility, viability, acrosome, DNA integrity, and phospholipase C zeta were detected by different established methods. Different techniques were used to assess different parameters. Motility was detected using computer assisted sperm analysis, phospholipase C zeta using luminometry while viability, acrosome integrity, and DNA integrity were analyzed using flow cytometry. Thawing conditions were noted to have an effect on sperm quality parameters with motility being the most critical parameter. The results further indicated that the most critical step during cryopreservation of boar semen is when sperm cells are subjected to freezing and thawing. The findings of the present study provide insight that; boar semen cryopreservation is still suboptimal in comparison to bull semen cryopreservation. Thus, there is a need to conduct more research to improve the fertilizing potential of cryopreserved boar semen. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cryopreservation" title="cryopreservation">cryopreservation</a>, <a href="https://publications.waset.org/abstracts/search?q=computer%20assisted%20sperm" title=" computer assisted sperm"> computer assisted sperm</a>, <a href="https://publications.waset.org/abstracts/search?q=flow%20cytometry" title=" flow cytometry"> flow cytometry</a>, <a href="https://publications.waset.org/abstracts/search?q=luminometry" title=" luminometry"> luminometry</a> </p> <a href="https://publications.waset.org/abstracts/104731/cryoinjuries-in-sperm-cells-effect-of-adaptation-of-steps-in-cryopreservation-protocol-for-boar-semen-upon-post-thaw-sperm-quality" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104731.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">148</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">784</span> Effects of Adding Sodium Nitroprusside in Semen Diluents on Motility, Viability and Lipid Peroxidation of Sperm of Holstein Bulls</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leila%20Karshenas">Leila Karshenas</a>, <a href="https://publications.waset.org/abstracts/search?q=Hamid%20Reza%20Khodaei"> Hamid Reza Khodaei</a>, <a href="https://publications.waset.org/abstracts/search?q=Behnaz%20Mahdavi"> Behnaz Mahdavi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We know that nitric oxide (NO) plays an important role in all sexual activities of animals. It is made in body from NO synthase enzyme and L-arginin molecule. NO can bound with sulfur-iron complexes and because production of steroid sexual hormones is related to enzymes which have this complex, NO can change the activity of these enzymes. NO affects many cells including endothelial cells of veins, macrophages and mast cells. These cells are found in testis leydig cells and therefore are important source of NO in testis tissue. Minimizing damages to sperm at the time of sperm freezing and thawing is really important. The goal of this study was to determine the function of NO before freezing and its effects on quality and viability of sperms after thawing and incubation. 4 Holstein bulls were selected from the age of 4, and artificial insemination was done for 3 weeks (2 times a week). Treatments were 0, 10, 50 and 100 nm of sodium nitroprusside (SNP). Data analysis was performed by SAS98 program. Also, mean comparison was done using Duncan's multiple ranges test (P<0.05). Concentrations used was found to increase motility and viability of spermatozoa at 1, 2 and 3 hours after thawing significantly (P<0.05), but there was no significant difference at zero time. SNP levels reduced the amount of lipid peroxidation in sperm membrane, increased acrosome health and improved sample membranes especially in 50 and 100 nm treatments. According to results, adding SNP to semen diluents increases motility and viability of spermatozoa. Also, it reduces lipid peroxidation in sperm membrane and improves sperm function. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sperm%20motility" title="sperm motility">sperm motility</a>, <a href="https://publications.waset.org/abstracts/search?q=nitric%20oxide" title=" nitric oxide"> nitric oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20peroxidation" title=" lipid peroxidation"> lipid peroxidation</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatozoa" title=" spermatozoa"> spermatozoa</a> </p> <a href="https://publications.waset.org/abstracts/12296/effects-of-adding-sodium-nitroprusside-in-semen-diluents-on-motility-viability-and-lipid-peroxidation-of-sperm-of-holstein-bulls" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12296.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">360</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">783</span> Protein and MDA (Malondialdehyde) Profil of Bull Sperm and Seminal Plasma After Freezing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sri%20Rahayu">Sri Rahayu</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Dwi%20Susan"> M. Dwi Susan</a>, <a href="https://publications.waset.org/abstracts/search?q=Aris%20Soewondo"> Aris Soewondo</a>, <a href="https://publications.waset.org/abstracts/search?q=W.%20M.%20Agung%20Pramana"> W. M. Agung Pramana</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Semen is an organic fluid (seminal plasma) that contain spermatozoa. Proteins are one of the major seminal plasma components that modulate sperm functionality, influence sperm capacitation and maintaining the stability of the membrane. Semen freezing is a procedure to preserve sperm cells. The process causes decrease in sperm viability due to temperature shock and oxidation stress. Oxidation stress is a disturbance on phosphorylation that increases ROS concentration, and it produces lipid peroxide in spermatozoa membrane resulted in high MDA (malondialdehyde) concentration. The objective of this study was to examine the effect of freezing on protein and MDA profile of bovine sperm cell and seminal plasma after freezing. Protein and MDA of sperm cell and seminal plasma were isolated from 10 sample. Protein profiles was analyzed by SDS PAGE with separating gel 12,5 %. The concentration of MDA was measured by spectrophotometer. The results of the research indicated that freezing of semen cause lost of the seminal plasma proteins with molecular with 20, 10, and 9 kDa. In addition, the result research showed that protein of the sperm (26, 10, 9, 7, and 6 kDa) had been lost. There were difference MDA concentration of seminal plasma and sperm cell were increase after freezing. MDA concentration of seminal plasma before and after freezing were 2.2 and 2.4 nmol, respectively. MDA concentration of sperm cell before and after freezing were 1,5 and 1.8 nmol, respectively. In conclusion, there were differences protein profiles of spermatozoa before and after semen freezing and freezing cause increasing of the MDA concentration. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=MDA" title="MDA">MDA</a>, <a href="https://publications.waset.org/abstracts/search?q=semen%20freezing" title=" semen freezing"> semen freezing</a>, <a href="https://publications.waset.org/abstracts/search?q=SDS%20PAGE" title=" SDS PAGE"> SDS PAGE</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20profile" title=" protein profile"> protein profile</a> </p> <a href="https://publications.waset.org/abstracts/9455/protein-and-mda-malondialdehyde-profil-of-bull-sperm-and-seminal-plasma-after-freezing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/9455.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">275</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">782</span> Spermiogram Values of Fertile Men in Malatya Region</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aliseydi%20Bozkurt">Aliseydi Bozkurt</a>, <a href="https://publications.waset.org/abstracts/search?q=Ugur%20Y%C4%B1lmaz"> Ugur Yılmaz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: It was aimed to evaluate the current status of semen parameters in fertile males with one or more children and whose wife having a pregnancy for the last 1-12 months in Malatya region. Methods: Sperm samples were obtained from 131 voluntary fertile men. In each analysis, sperm volume (ml), number of sperm (sperm/ml), sperm motility and sperm viscosity were examined with Makler device. Classification was made according to World Health Organization (WHO) criteria. Results: Mean ejaculate volume ranged from 1.5 ml to 5.5 ml, sperm count ranged from 27 to 180 million/ml and motility ranged from 35 to 90%. Sperm motility was found to be on average; 69.9% in A, 7.6% in B, 8.7% in C, 13.3% in D category. Conclusion: The mean spermiogram values of fertile males in Malatya region were found to be similar to those in fertile males determined by the WHO. This study has a regional classification value in terms of spermiogram values. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fertile%20men" title="fertile men">fertile men</a>, <a href="https://publications.waset.org/abstracts/search?q=infertility" title=" infertility"> infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=spermiogram" title=" spermiogram"> spermiogram</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20motility" title=" sperm motility"> sperm motility</a> </p> <a href="https://publications.waset.org/abstracts/96646/spermiogram-values-of-fertile-men-in-malatya-region" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96646.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">352</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">781</span> Effect of Electromagnetic Radiation on Reproductive System of Male Rat</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rohit%20Gautam">Rohit Gautam</a>, <a href="https://publications.waset.org/abstracts/search?q=Kumari%20Vandana%20Singh"> Kumari Vandana Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Jayprakash%20Nirala"> Jayprakash Nirala</a>, <a href="https://publications.waset.org/abstracts/search?q=Nina%20Nancy%20Murmu"> Nina Nancy Murmu</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramovatar%20Meena"> Ramovatar Meena</a>, <a href="https://publications.waset.org/abstracts/search?q=Paulraj%20Rajamani"> Paulraj Rajamani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mobile phones have become a vital part of everyone’s life. Mobile phone and mobile phone towers emit RF-EMR (Radiofrequency Electromagnetic Radiation), which becomes a cause of concern to the general public. The study was designed to evaluate the effect of 3G (RF-EMR) on the reproductive system of male Wistar rats. Adult male Wistar rats were used for the study. Animals were divided into two groups, RF-exposed, and sham-exposed (control). RF-exposed rats were exposed to radio frequency radiation (2100 MHz) for 2 hours/day for 45 days. Emitted power density and specific absorption rate (SAR) values were measured during exposure. At the end of the exposure, testis and epididymis were excised out, and their weights were recorded. Sperm cell count, morphology, viability, and reactive oxygen species (ROS) levels were checked. Lipid peroxidation and sperm mitochondrial activity were measured. Histopathology of testis and ultrastructure analysis of sperm were also checked. Result showed a decrease in organ weight and sperm count with alteration in the sperm morphology in exposed group rats. A significant decrease in sperm viability, membrane integrity, and mitochondrial activity was found. Also, an increase in lipid peroxidation and ROS level were found in exposed group animals as compared to control. It may be concluded that exposure to radiofrequency radiation emits from mobile phones leads to oxidative stress-mediated changes in reproductive parameters. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electromagnetic%20radiation" title="electromagnetic radiation">electromagnetic radiation</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a>, <a href="https://publications.waset.org/abstracts/search?q=reactive%20oxygen%20species" title=" reactive oxygen species"> reactive oxygen species</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm" title=" sperm"> sperm</a> </p> <a href="https://publications.waset.org/abstracts/117774/effect-of-electromagnetic-radiation-on-reproductive-system-of-male-rat" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/117774.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">178</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">780</span> Sustaining the Mitochondrial Transcription Factor A in Sperm</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Betty%20Anson">Betty Anson</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Researchers have found that mature sperm cells are not only devoid of mature MTDNA (mitochondrial DNA) but also lack a particular protein essential for DNA maintenance, known as mitochondrial transcription factor A, or TFAM (transcription factor A mitochondria). As a result, children get the DNA of certain important body functions only from their mothers. More experiments show that TFAM appears to burn out when it is used as a source of energy for sperm movement. This study investigates alternative sources of energy for sperm movement that could sustain the existence of TFAM. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mItochondria" title="mItochondria">mItochondria</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA" title=" DNA"> DNA</a>, <a href="https://publications.waset.org/abstracts/search?q=TFAM" title=" TFAM"> TFAM</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm" title=" sperm"> sperm</a> </p> <a href="https://publications.waset.org/abstracts/173267/sustaining-the-mitochondrial-transcription-factor-a-in-sperm" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173267.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">74</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">779</span> In vitro Effects of Amygdalin on the Functional Competence of Rabbit Spermatozoa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marek%20Halen%C3%A1r">Marek Halenár</a>, <a href="https://publications.waset.org/abstracts/search?q=Eva%20Tvrd%C3%A1"> Eva Tvrdá</a>, <a href="https://publications.waset.org/abstracts/search?q=Tom%C3%A1%C5%A1%20Slanina"> Tomáš Slanina</a>, <a href="https://publications.waset.org/abstracts/search?q=%C4%BDubom%C3%ADr%20Ondru%C5%A1ka"> Ľubomír Ondruška</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduard%20Koles%C3%A1r"> Eduard Kolesár</a>, <a href="https://publications.waset.org/abstracts/search?q=Peter%20Mass%C3%A1nyi"> Peter Massányi</a>, <a href="https://publications.waset.org/abstracts/search?q=Adriana%20Koles%C3%A1rov%C3%A1"> Adriana Kolesárová </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present <em>in vitro</em> study was designed to reveal whether amygdalin (AMG) is able to cause changes to the motility, viability and mitochondrial activity of rabbit spermatozoa. New Zealand White rabbits (n = 10) aged four months were used in the study. Semen samples were collected from each animal and used for the <em>in vitro </em>incubation. The samples were divided into five equal parts and diluted with saline supplemented with 0, 0.5, 1, 2.5 and 5 mg/mL AMG. At times 0h, 3h and 5h spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system, cell viability was examined with the metabolic activity (MTT) assay, and the eosin-nigrosin staining technique was used to evaluate the viability of rabbit spermatozoa. All AMG concentrations exhibited stimulating effects on the spermatozoa activity, as shown by a significant preservation of the motility (P<0.05 with respect to 0.5 mg/mL and 1 mg/mL AMG; Time 5 h) and mitochondrial activity (P< 0.05 in case of 0.5 mg/mL AMG; P< 0.01 in case of 1 mg/mL AMG; P < 0.001 with respect to 2.5 mg/mL and 5 mg/mL AMG; Time 5 h). None of the AMG doses supplemented had any significant impact of the spermatozoa viability. In conclusion, the data revealed that short-term co-incubation of spermatozoa with AMG may result in a higher preservation of the sperm structural integrity and functional activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=amygdalin" title="amygdalin">amygdalin</a>, <a href="https://publications.waset.org/abstracts/search?q=CASA" title=" CASA"> CASA</a>, <a href="https://publications.waset.org/abstracts/search?q=mitochondrial%20activity" title=" mitochondrial activity"> mitochondrial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=motility" title=" motility"> motility</a>, <a href="https://publications.waset.org/abstracts/search?q=rabbits" title=" rabbits"> rabbits</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatozoa" title=" spermatozoa"> spermatozoa</a>, <a href="https://publications.waset.org/abstracts/search?q=viability" title=" viability"> viability</a> </p> <a href="https://publications.waset.org/abstracts/55018/in-vitro-effects-of-amygdalin-on-the-functional-competence-of-rabbit-spermatozoa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/55018.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">778</span> Sexual Health And Male Fertility: Improving Sperm Health With Focus On Technology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Diana%20Peninger">Diana Peninger</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Over 10% of couples in the U.S. have infertility problems, with roughly 40% traceable to the male partner. Yet, little attention has been given to improving men’s contribution to the conception process. One solution that is showing promise in increasing conception rates for IVF and other assisted reproductive technology treatments is a first-of-its-kind semen collection that has been engineered to mitigate sperm damage caused by traditional collection methods. Patients are able to collect semen at home and deliver to clinics within 48 hours for use in fertility analysis and treatment, with less stress and improved specimen viability. This abstract will share these findings along with expert insight and tips to help attendees understand the key role sperm collection plays in addressing and treating reproductive issues, while helping to improve patient outcomes and success. Our research was to determine if male reproductive outcomes can be increased by improving sperm specimen health with a focus on technology. We utilized a redesigned semen collection cup (patented as the Device for Improved Semen Collection/DISC—U.S. Patent 6864046 – known commercially as a ProteX) that met a series of physiological parameters. Previous research demonstrated significant improvement in semen perimeters (motility forward, progression, viability, and longevity) and overall sperm biochemistry when the DISC is used for collection. Animal studies have also shown dramatic increases in pregnancy rates. Our current study compares samples collected in the DISC, next-generation DISC (DISCng), and a standard specimen cup (SSC), dry, with the 1 mL measured amount of media and media in excess ( 5mL). Both human and animal testing will be included. With sperm counts declining at alarming rates due to environmental, lifestyle, and other health factors, accurate evaluations of sperm health are critical to understanding reproductive health, origins, and treatments of infertility. An increase in the health of the sperm as measured by extensive semen parameter analysis and improved semen parameters stable for 48 hours, expanding the processing time from 1 hour to 48 hours were also demonstrated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=reprodutive" title="reprodutive">reprodutive</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm" title=" sperm"> sperm</a>, <a href="https://publications.waset.org/abstracts/search?q=male" title=" male"> male</a>, <a href="https://publications.waset.org/abstracts/search?q=infertility" title=" infertility"> infertility</a> </p> <a href="https://publications.waset.org/abstracts/147626/sexual-health-and-male-fertility-improving-sperm-health-with-focus-on-technology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147626.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">129</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">777</span> The Evaluation of the Effects of Atypical Antipsychotics on Sperm Quality by Computer-Assisted Sperm Analysis in Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=O.%20Atli%20Eklioglu">O. Atli Eklioglu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Atypical antipsychotics such as quetiapine, olanzapine, and risperidone have been frequently and chronically used to treat psychiatric disorders accompanied by psychosis mainly schizophrenia. Since these drugs are commonly used in male patients of reproductive age, it is required to determine the possible effects of them on the reproductive system. In this study, it was aimed to evaluate the possible toxic effects of quetiapine, olanzapine and risperidone, which are the most frequently prescribed and chronically used psychiatric drugs, on sperm parameters. For this purpose, quetiapine (10, 20 and 40 mg/kg), olanzapine (2.5, 5 and 10 mg/kg), and risperidone (1.25, 2.5 and 3 mg/kg) were administered to male rats for 28 consecutive days. At the end of this period, sperm concentration, motility, and morphology were investigated by a computer-assisted sperm analysis system. According to the results, sperm parameters were negatively affected by antipsychotic use. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=quetiapine" title="quetiapine">quetiapine</a>, <a href="https://publications.waset.org/abstracts/search?q=olanzapine" title=" olanzapine"> olanzapine</a>, <a href="https://publications.waset.org/abstracts/search?q=risperidone" title=" risperidone"> risperidone</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20count" title=" sperm count"> sperm count</a>, <a href="https://publications.waset.org/abstracts/search?q=motility" title=" motility"> motility</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20morphology" title=" sperm morphology"> sperm morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=computer-assisted%20sperm%20analysis" title=" computer-assisted sperm analysis"> computer-assisted sperm analysis</a> </p> <a href="https://publications.waset.org/abstracts/97528/the-evaluation-of-the-effects-of-atypical-antipsychotics-on-sperm-quality-by-computer-assisted-sperm-analysis-in-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/97528.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">152</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">776</span> In vitro Effects of Salvia officinalis on Bovine Spermatozoa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eva%20Tvrd%C3%A1">Eva Tvrdá</a>, <a href="https://publications.waset.org/abstracts/search?q=Boris%20Botman"> Boris Botman</a>, <a href="https://publications.waset.org/abstracts/search?q=Marek%20Halen%C3%A1r"> Marek Halenár</a>, <a href="https://publications.waset.org/abstracts/search?q=Tom%C3%A1%C5%A1%20Slanina"> Tomáš Slanina</a>, <a href="https://publications.waset.org/abstracts/search?q=Norbert%20Luk%C3%A1%C4%8D"> Norbert Lukáč</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <em>In vitro</em> storage and processing of animal semen represents a risk factor to spermatozoa vitality, potentially leading to reduced fertility. A variety of substances isolated from natural sources may exhibit protective or antioxidant properties on the spermatozoon, thus extending the lifespan of stored ejaculates. This study compared the ability of different concentrations of the <em>Salvia officinalis</em> extract on the motility, mitochondrial activity, viability and reactive oxygen species (ROS) production by bovine spermatozoa during different time periods (0, 2, 6 and 24 h) of <em>in vitro</em> culture. Spermatozoa motility was assessed using the Computer-assisted sperm analysis (CASA) system. Cell viability was examined using the metabolic activity MTT assay, the eosin-nigrosin staining technique was used to evaluate the sperm viability and ROS generation was quantified using luminometry. The CASA analysis revealed that the motility in the experimental groups supplemented with 0.5-2 µg/mL <em>Salvia</em> extract was significantly lower in comparison with the control (P<0.05; Time 24 h). At the same time, a long-term exposure of spermatozoa to concentrations ranging between 0.05 µg/mL and 2 µg/mL had a negative impact on the mitochondrial metabolism (P<0.05; Time 24 h). The viability staining revealed that 0.001-1 µg/mL <em>Salvia</em> extract had no effects on bovine male gametes, however 2 µg/mL <em>Salvia</em> had a persisting negative effect on spermatozoa (P<0.05). Furthermore 0.05-2 µg/mL <em>Salvia</em> exhibited an immediate ROS-promoting effect on the sperm culture (P>0.05; Time 0 h and 2 h), which remained significant throughout the entire <em>in vitro</em> culture (P<0.05; Time 24 h). Our results point out to the necessity to examine specific effects the biomolecules present in <em>Salvia officinalis</em> may have individually or collectively on the <em>in vitro</em> sperm vitality and oxidative profile. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bulls" title="bulls">bulls</a>, <a href="https://publications.waset.org/abstracts/search?q=CASA" title=" CASA"> CASA</a>, <a href="https://publications.waset.org/abstracts/search?q=MTT%20test" title=" MTT test"> MTT test</a>, <a href="https://publications.waset.org/abstracts/search?q=reactive%20oxygen%20species" title=" reactive oxygen species"> reactive oxygen species</a>, <a href="https://publications.waset.org/abstracts/search?q=sage" title=" sage"> sage</a>, <a href="https://publications.waset.org/abstracts/search?q=Salvia%20officinalis" title=" Salvia officinalis"> Salvia officinalis</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatozoa" title=" spermatozoa"> spermatozoa</a> </p> <a href="https://publications.waset.org/abstracts/56562/in-vitro-effects-of-salvia-officinalis-on-bovine-spermatozoa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56562.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">338</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">775</span> Seminal Attributes, Cooling Procedure and Post Thaw Quality of Semen of Indigenous Khari Bucks (Capra hircus) of Nepal</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pankaj%20Kumar%20Jha">Pankaj Kumar Jha</a>, <a href="https://publications.waset.org/abstracts/search?q=Saroj%20Sapkota"> Saroj Sapkota</a>, <a href="https://publications.waset.org/abstracts/search?q=Dil%20Bahadur%20%20Gurung"> Dil Bahadur Gurung</a>, <a href="https://publications.waset.org/abstracts/search?q=Raju%20Kadel"> Raju Kadel</a>, <a href="https://publications.waset.org/abstracts/search?q=Neena%20Amatya%20Gorkhali"> Neena Amatya Gorkhali</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhola%20Shankar%20Shrestha"> Bhola Shankar Shrestha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study was conducted to evaluate the seminal attributes, effectiveness of cooling process and post-thawed semen quality of a Nepalese indigenous Khari buck. Thirty-two ejaculates, 16 from each buck were studied for seminal attributes of fresh semen: volume, color, mass activity, motility, viability, sperm concentration, and morphology. The pooled mean values for each seminal attributes were: volume 0.7±0.3 ml; colour 3.1±0.3 (milky white); mass activity 3.8±0.4 (rapid wave motion with formation of eddies at the end of waves to very rapid wave motion with distinct eddies formation); sperm motility 80.9±5.6%; sperm viability 94.6±2.0%; sperm concentration 2597.0±406.8x106/ml; abnormal acrosome, mid-piece and tail 10.7±1.8% and abnormal head 5±1.7%. For freezing semen, further 6 ejaculates from each buck were studied with Tris based egg yolk citrate extender. The pooled mean values of motility and viability of post diluted semen for 90 and 120 minutes each for cooling and glycerol equilibration were 73.8±4.8%, 88.1±2.6% and 69.2±6.0%, 85.0±1.7%, respectively. The pooled mean values of post thaw motility and viability with advancement of preservation time were: 0hour 49.0±4.6%, 81.2±1.9%; 2nd day 41±2.2%, 79±1%; 5th day 41±2.2%, 78.6±0.9% and 10th day 41±2.2%, 78.6±0.9%. We concluded from the above study that the seminal attributes and results of post-thaw semen quality were satisfactory and in accordance with other work in foreign countries, which indicated the feasibility of cryopreserving buck semen. For more validation, research with large number of bucks, different types of diluents and freezing trials by removing seminal plasma followed by pregnancy rate is recommended. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cryopreservation" title="cryopreservation">cryopreservation</a>, <a href="https://publications.waset.org/abstracts/search?q=Nepalese%20indigenous%20Khari%20%28Hill%20goat%29%20buck" title=" Nepalese indigenous Khari (Hill goat) buck"> Nepalese indigenous Khari (Hill goat) buck</a>, <a href="https://publications.waset.org/abstracts/search?q=post-thaw%20semen%20quality" title=" post-thaw semen quality"> post-thaw semen quality</a>, <a href="https://publications.waset.org/abstracts/search?q=seminal%20attributes" title=" seminal attributes"> seminal attributes</a> </p> <a href="https://publications.waset.org/abstracts/62710/seminal-attributes-cooling-procedure-and-post-thaw-quality-of-semen-of-indigenous-khari-bucks-capra-hircus-of-nepal" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/62710.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">402</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">774</span> Non-thermal Plasma Promotes Boar Sperm Quality Through Increasing AMPK Methylation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jiaojiao%20Zhang">Jiaojiao Zhang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Boar sperm quality, as an important indicator of reproductive efficiency, directly affects the efficiency of livestock production. Here, this study was conducted to improve the boar sperm quality by using a non-thermal dielectric barrier discharge (DBD) plasma. Our results showed that DBD plasma exposure at 2.1 W for 15 s could improve boar sperm quality by increasing the exon methylation level of adenosine monophosphate-activated protein kinase (AMPK) and thus improving the glycolytic flux, mitochondrial function, and antioxidant capacity without damaging the integrity of sperm DNA and acrosome. In addition, DBD plasma could rescue DNA methyltransferase inhibitor decitabine-caused low sperm quality by reducing oxidative stress and mitochondrial damage. Therefore, the application of non-thermal plasma provides a new strategy for reducing sperm oxidative damage and improving sperm quality, which shows great potential in assisted reproduction to solve the problem of male infertility. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=non-thermal%20DBD%20plasma" title="non-thermal DBD plasma">non-thermal DBD plasma</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20quality" title=" sperm quality"> sperm quality</a>, <a href="https://publications.waset.org/abstracts/search?q=AMPK%20methylation" title=" AMPK methylation"> AMPK methylation</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20metabolism" title=" energy metabolism"> energy metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20capacity" title=" antioxidant capacity"> antioxidant capacity</a> </p> <a href="https://publications.waset.org/abstracts/193854/non-thermal-plasma-promotes-boar-sperm-quality-through-increasing-ampk-methylation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193854.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">9</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">773</span> Investigation of Some Sperm Quality Parameters of Farmed and Wild-Caught Meagre (Argyrosomus regius Asso, 1801)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=%C5%9Eefik%20Surhan%20Tabako%C4%9Flu">Şefik Surhan Tabakoğlu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hipolito%20Fern%C3%A1ndez-Palacios"> Hipolito Fernández-Palacios</a>, <a href="https://publications.waset.org/abstracts/search?q=Dominique%20Schuchardt"> Dominique Schuchardt</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmut%20Ali%20G%C3%B6k%C3%A7e"> Mahmut Ali Gökçe</a>, <a href="https://publications.waset.org/abstracts/search?q=Celal%20Erba%C5%9F"> Celal Erbaş</a>, <a href="https://publications.waset.org/abstracts/search?q=O%C4%9Fuz%20Ta%C5%9Fbozan"> Oğuz Taşbozan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study aimed to clarify some sperm quality parameters such as volumetric sperm quantity, motility, motility duration, sperm density, total number of spermatozoa and pH of meagre (Argyrosomus regius ASSO, 1801) individuals kept in farming conditions and caught from wild (las palmas, gran canary). The sperm was collected in glass tubes graded in millimetres and sperm volume registered immediately following collection by abdominal massage. The sperm quality parameters including motility, total number of spermatozoa and spermatozoa density were determined with computer assisted sperm analysis (CASA) program. The duration of spermatozoa movement was assessed using a sensitive chronometer (1/100s) that was started simultaneously with the addition of activation solution into the sample. Sperm pH was measured with standard pH electrodes within five minutes of sampling. At the end of the study, while amount of sperm (5.20±0.33 ml), duration of motility (7.23±0.7 m) and total number of spermatozoa (131.40±12.22 x10^9) were different statistically (p < 0,05), motility (% 81.03±6.59), pH (7.30±0.08), sperm density (25.27±9.42 x10^9/ml) and morphologic parameters were not significantly different between the two groups. According to our results, amount of sperm, duration of motility and total number of spermatozoa were better in farmed group than that of the other group. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seriola%20rivoliana" title="Seriola rivoliana">Seriola rivoliana</a>, <a href="https://publications.waset.org/abstracts/search?q=meagre" title=" meagre"> meagre</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20quality" title=" sperm quality"> sperm quality</a>, <a href="https://publications.waset.org/abstracts/search?q=motility" title=" motility"> motility</a>, <a href="https://publications.waset.org/abstracts/search?q=motility%20duration" title=" motility duration"> motility duration</a> </p> <a href="https://publications.waset.org/abstracts/67842/investigation-of-some-sperm-quality-parameters-of-farmed-and-wild-caught-meagre-argyrosomus-regius-asso-1801" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67842.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">375</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">772</span> Comparative Evaluation of Different Extenders and Sperm Protectors to Keep the Spermatozoa Viable for More than 24 Hours</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20M.%20Raseona">A. M. Raseona</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20M.%20Barry"> D. M. Barry</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20L.%20Nedambale"> T. L. Nedambale</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Preservation of semen is an important process to ensure that semen quality is sufficient for assisted reproductive technology. This study evaluated the effectiveness of different extenders to preserve Nguni bull semen stored at controlled room temperature 24 °C for three days, as an alternative to frozen-thawed semen straws used for artificial insemination. Semen samples were collected from two Nguni bulls using an electro-ejaculator and transported to the laboratory for evaluation. Pooled semen was aliquot into three extenders Triladyl, Ham’s F10 and M199 at a dilution ratio of 1:4 then stored at controlled room temperature 24 °C. Sperm motility was analysed after 0, 24, 48 and 72 hours. Morphology and viability were analysed after 72 hours. The study was replicated four times and data was analysed by analysis of variance (ANOVA). Triladyl showed higher viability percentage and consistent total motility for three days. Ham’s F10 showed higher progressive motility compared to the other extenders. There was no significant difference in viability between Ham’s F10 and M199. No significant difference was also observed in total abnormality between the two Nguni bulls. In conclusion, Nguni semen can be preserved in Triladyl or Ham’s F10 and M199 culture media stored at 24 °C and stay alive for three days. Triladyl proved to be the best extender showing high viability and consistency in total motility as compared to Ham’s F10 and M199. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bull%20semen" title="bull semen">bull semen</a>, <a href="https://publications.waset.org/abstracts/search?q=artificial%20insemination" title=" artificial insemination"> artificial insemination</a>, <a href="https://publications.waset.org/abstracts/search?q=Triladyl" title=" Triladyl"> Triladyl</a>, <a href="https://publications.waset.org/abstracts/search?q=Ham%E2%80%99s%20F10" title=" Ham’s F10"> Ham’s F10</a>, <a href="https://publications.waset.org/abstracts/search?q=M199" title=" M199"> M199</a>, <a href="https://publications.waset.org/abstracts/search?q=viability" title=" viability"> viability</a> </p> <a href="https://publications.waset.org/abstracts/28623/comparative-evaluation-of-different-extenders-and-sperm-protectors-to-keep-the-spermatozoa-viable-for-more-than-24-hours" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28623.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">500</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">771</span> Impact of Propolis on Cryopreservation of Arctic Charr (Salvelinus alpinus) Sperm</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20A.%20El-Battawy">K. A. El-Battawy</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Brannas"> E. Brannas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cryopreservation of sperm causes damages and adversely affected sperm motility and viability resulting in lower hatching rates. The aim of this study is to determine whether propolis has potential protective effect on cryopreservation and fertilization ability of spermatozoa of Salvelinusalpinus. The extenders were prepared by using simple glucose solution (0.3 M glucose) to which 10% Me2SO added with different levels of propolis (0.4, 0.8 and 1 mg/ ml) and 10% egg yolk (as a control without propolis). The pooled semen samples diluted at the ratio of 1:3 by the extenders were subjected to cryopreservation. The percentage and duration of motility and fertilization tests of cryopreserved sperm samples have been done immediately after thawing and compared with control and fresh semen. The extenders containing propolis showed higher percentage motility and motility duration than control group (P < 0.05). Especially the group II (0.8 mg/ ml propolis) and the group III (1 mg/ ml propolis) showed significant positive effects on both post thaw motility and hatching ability. In conclusion, this study confirms that the propolis is an appropriate cryoptrotective agent in fish semen and it maintained the integrity of the spermatozoa during the cryopreservation process. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=propolis" title="propolis">propolis</a>, <a href="https://publications.waset.org/abstracts/search?q=arctic%20charr" title=" arctic charr"> arctic charr</a>, <a href="https://publications.waset.org/abstracts/search?q=semen" title=" semen"> semen</a>, <a href="https://publications.waset.org/abstracts/search?q=cryopreservation" title=" cryopreservation"> cryopreservation</a> </p> <a href="https://publications.waset.org/abstracts/41789/impact-of-propolis-on-cryopreservation-of-arctic-charr-salvelinus-alpinus-sperm" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41789.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">286</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">770</span> Old Swimmers Tire Quickly: The Effect of Time on Quality of Thawed versus Washed Sperm</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Emily%20Hamilton">Emily Hamilton</a>, <a href="https://publications.waset.org/abstracts/search?q=Adiel%20Kahana"> Adiel Kahana</a>, <a href="https://publications.waset.org/abstracts/search?q=Ron%20Hauser"> Ron Hauser</a>, <a href="https://publications.waset.org/abstracts/search?q=Shimi%20Barda"> Shimi Barda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> BACKGROUND: In the male fertility and sperm bank unit of Tel Aviv Sourasky medical center, women are treated with intrauterine insemination (IUI) using washed sperm from their partner or thawed sperm from a selected donor. In most cases, the women perform the IUI treatment in Sourasky, but sometimes they ask to undergo the insemination procedure in another clinic with their own fertility doctor. In these cases, the sperm sample is prepared at the Sourasky lab and the patient is inseminated after arriving to her doctor. Our laboratory has previously found that time negatively affects several parameters of thawed sperm, and we estimate that it has more severe and significant effect than on washed sperm. AIM: To examine the effect of time on the quality of washed sperm versus thawed sperm. METHODS: Sperm samples were collected from men referred for semen analysis. Each ejaculate was allowed to liquefy for at least 20 min at 37°C and analyzed for sperm motility and vitality percentage and DNA fragmentation index (Time 0). Subsequently, 1ml of the sample was divided into two parts, 1st part was washed only and the 2nd part was washed, frozen and thawed. Time 1 analysis occurred immediately after sperm washing or thawing. Time 2 analysis occurred 75 minutes after time 1. Statistical analysis was performed using Student t-test. P values<0.05 were considered significant. RESULTS: Preliminary data showed that time had a greater impact on the average percentages of sperm motility and vitality in thawed compared to washed sperm samples (26%±10% vs. 21%±10% and 21%±9% vs. 9%±10%, respectively). An additional trend towards increased average DNA fragmentation percentage in thawed samples compared to washed samples was observed (46%±18% vs. 25%±24%). CONCLUSION: Time negatively effects sperm quality. The effect is greater in thawed samples compared to fresh samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ART" title="ART">ART</a>, <a href="https://publications.waset.org/abstracts/search?q=male%20fertility" title=" male fertility"> male fertility</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20cryopreservation" title=" sperm cryopreservation"> sperm cryopreservation</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20quality" title=" sperm quality"> sperm quality</a> </p> <a href="https://publications.waset.org/abstracts/136935/old-swimmers-tire-quickly-the-effect-of-time-on-quality-of-thawed-versus-washed-sperm" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/136935.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">194</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">769</span> Antioxidants Effects on Sperm Parameter in Varicocelized Male Rat</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehdi%20Abbasi">Mehdi Abbasi</a>, <a href="https://publications.waset.org/abstracts/search?q=Masoumeh%20Majidi%20Zolbin"> Masoumeh Majidi Zolbin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Varicocele is one of the common causes of infertility in 30-50% of married men which occurs within the spermatic cord. It can be considered as an abnormal dilatation and stasis of veins of the pampiniform plexus that drain the testis. It occurs in 15-20% of the male population. Inducible nitric oxide synthase (NOS) activity has been frequently reported in varicose veins. Several studies have considered the relationship between varicocele and semen NO concentrations. NOS isoforms have been shown to regulate a number of functions, e.g., sperm motility and maturation and germ cell apoptosis in the testes. In adult patients with varicocele, the amount of NO levels in the varicose veins are 25 times higher than in serum of peripheral veins. The aim of this study was to review the effect of different antioxidant that we applied so far on sperm parameters as well as sperm DNA fragmentation. The findings of this study suggest that antioxidants improve sperm parameters which are associated with infertility in varicocelized rats, and treatment can reduce damage to sperm DNA and increase the chance of fertility. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title="antioxidant">antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=rat" title=" rat"> rat</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20parameter" title=" sperm parameter"> sperm parameter</a>, <a href="https://publications.waset.org/abstracts/search?q=varicocele" title=" varicocele"> varicocele</a> </p> <a href="https://publications.waset.org/abstracts/84230/antioxidants-effects-on-sperm-parameter-in-varicocelized-male-rat" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84230.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">279</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">768</span> Region-Specific Secretory Protein, α2M, in Male Reproductive Tract of the Blue Crab And Its Dynamics during Sperm transit towards Female Spermatheca</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thanyaporn%20Senarai">Thanyaporn Senarai</a>, <a href="https://publications.waset.org/abstracts/search?q=Rapeepun%20Vanichviriyakit"> Rapeepun Vanichviriyakit</a>, <a href="https://publications.waset.org/abstracts/search?q=Shinji%20Miyata"> Shinji Miyata</a>, <a href="https://publications.waset.org/abstracts/search?q=Chihiro%20Sato"> Chihiro Sato</a>, <a href="https://publications.waset.org/abstracts/search?q=Prapee%20Sretarugsa"> Prapee Sretarugsa</a>, <a href="https://publications.waset.org/abstracts/search?q=Wattana%20Weerachatyanukul"> Wattana Weerachatyanukul</a>, <a href="https://publications.waset.org/abstracts/search?q=Ken%20Kitajima"> Ken Kitajima</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we characterized a region-specific 250 kDa protein that was secreted of MSD fluid, which is believed to play dual functions in forming a spermatophoric wall for sperm physical protection, and in sperm membrane modification as part of sperm maturation process. The partial amino acid sequence and N-terminal sequencing revealed that the MSD-specific 250 kDa protein showed a high similarity with a plasma-rich protein, α-2 macroglobulin (α2M), so termed pp-α2M. This protein was a large glycoprotein contained predominantly mannose and GlcNAc. The expression of pp-α2M mRNA was detected in spermatic duct (SD), androgenic gland (AG) and hematopoietic tissue, while the protein expression was rather specific to the apical cytoplasm of MSD epithelium. The secretory pp-α2M in MSD fluid was acquired onto the MSD sperm membrane and was also found within the matrix of the acrosome. Distally, pp-α2M was removed from spermathecal sperm membrane, while its level kept constant in the sperm AC. Together the results indicate that pp-α2M is a 250 kDa region-specific secretory protein which plays roles in sperm physical protection and also acts as maturation factor in the P. pelagicus sperm. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha-2%20macroglobulin" title="alpha-2 macroglobulin">alpha-2 macroglobulin</a>, <a href="https://publications.waset.org/abstracts/search?q=blue%20swimming%20crab" title=" blue swimming crab"> blue swimming crab</a>, <a href="https://publications.waset.org/abstracts/search?q=sperm%20maturation" title=" sperm maturation"> sperm maturation</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatic%20duct" title=" spermatic duct "> spermatic duct </a> </p> <a href="https://publications.waset.org/abstracts/60090/region-specific-secretory-protein-a2m-in-male-reproductive-tract-of-the-blue-crab-and-its-dynamics-during-sperm-transit-towards-female-spermatheca" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60090.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">329</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">767</span> The Post Thawing Quality of Boer Goat Semen after Freezing by Mr. Frosty System Using Commercial Diluter </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gatot%20Ciptadi">Gatot Ciptadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mudawamah"> Mudawamah</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20P.%20Putra"> R. P. Putra</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Wahjuningsih"> S. Wahjuningsih</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20M.%20Munazaroh"> A. M. Munazaroh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The success rate of Artificial Insemination (AI) application, particularly in the field at the farmer level is highly dependent on the quality of the sperms one post thawing. The objective of this research was to determine the effect of freezing method (-1oC/ minute) using Mr. Frosty system with commercial diluents on the post-thawing quality of Boer goat semen. Method use is experimental design with the completely randomized design (CRD) with 4 treatments of commercial diluter percentage (v/v). Freezing semen was cryopreserved in 2 main final temperatures of –45 oC (Freezer) and –196 oC (liquid nitrogen). Result showed that different commercial diluter is influenced on viability motility and abnormalities of Boer semen. Pre-freezing qualities of viability, motilities and abnormalities was 88.67+4.16 %, 66.33 +1.53 % and 4.67+ 0.57 % respectively. Meanwhile, post-thawing qualities is considered as good as standard qualities at least more than 40 % (51.0+6.5%). The percentage of commercial diluents were influenced highly significant (P<0.01).The best diluents ration is 1:4 (v/v) for both final sperms stocked. However freezing sperm conserved in -196 oC is better than –45 oC (i.e. motility 39.3.94 % vs. 51.0 + 6.5 %). It was concluded that Mr. frosty system was considered as the feasible method for freezing semen in the reason for practical purposes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sperm%20quality" title="sperm quality">sperm quality</a>, <a href="https://publications.waset.org/abstracts/search?q=goat" title=" goat"> goat</a>, <a href="https://publications.waset.org/abstracts/search?q=viability" title=" viability"> viability</a>, <a href="https://publications.waset.org/abstracts/search?q=diluteR" title=" diluteR"> diluteR</a> </p> <a href="https://publications.waset.org/abstracts/39622/the-post-thawing-quality-of-boer-goat-semen-after-freezing-by-mr-frosty-system-using-commercial-diluter" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39622.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">259</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">766</span> Influence of Age on Some Testicular and Spermatic Parameters in Kids and Bucks in Local Breed Arbia in Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Boukhalfa%20Djemouai">Boukhalfa Djemouai</a>, <a href="https://publications.waset.org/abstracts/search?q=Belkadi%20Souhila"> Belkadi Souhila</a>, <a href="https://publications.waset.org/abstracts/search?q=Safsaf%20Boubakeur"> Safsaf Boubakeur</a> </p> <p class="card-text"><strong>Abstract:</strong></p> To increase the profitability of the national herd so that it can meet the needs of the population, Algeria has proceeded to the introduction of new reproductive biotechnologies, including artificial insemination on natural heat, by induction and heat synchronization. This biotechnology uses the male way for the creation and dissemination of genetic progress. The study has focused on 30 goat kids and bucks local breed aged between 03 and 24 months, divided into 03 groups 03-06 months[Grp 1; n=9], 07-10 months [Grp 2; n=13] and 11-24 months [Grp 3; n=8], in order to determine the influence of age on testicular evolution by measurements of testis and scrotum, and the epididymis sperm parameters evaluation. These parameters are influenced by age variations (sperm and spermocytogram). The examined parameters have focused on testicular weight (grams), the scrotal circumference (cm), mass mobility (%), vitality rate (%), sperm concentration (x 109), and percentage of abnormal spermatozoa (%). The ANOVA reveals a significance effect of age on parameters: testis weight, scrotal circumference, sperm concentration, motility varying between high (p < 0.01) to very high significance (p < 0.001), while in viability and abnormalities no significance was observed between all groups. The value of these parameters increased significantly until the age of 02 years, while that of sperm abnormalities has increased in Grp2. The histological study of testicular development shows that the genetic spermatozoa function characterized by cell proliferation, which is more and more intense starting from the age of 05 months and can be considered as an age of puberty in the local breed goat Arbia and increases with animal age. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=kids%20and%20bucks" title="kids and bucks">kids and bucks</a>, <a href="https://publications.waset.org/abstracts/search?q=epididymis%20sperm" title=" epididymis sperm"> epididymis sperm</a>, <a href="https://publications.waset.org/abstracts/search?q=testicular%20measurements" title=" testicular measurements"> testicular measurements</a>, <a href="https://publications.waset.org/abstracts/search?q=Arbia%20breed" title=" Arbia breed"> Arbia breed</a> </p> <a href="https://publications.waset.org/abstracts/125017/influence-of-age-on-some-testicular-and-spermatic-parameters-in-kids-and-bucks-in-local-breed-arbia-in-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/125017.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">132</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">765</span> Assessment of Sperm Aneuploidy Using Advanced Sperm Fish Technique in Infertile Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Archana%20S.">Archana S.</a>, <a href="https://publications.waset.org/abstracts/search?q=Usha%20Rani%20G."> Usha Rani G.</a>, <a href="https://publications.waset.org/abstracts/search?q=Anand%20Balakrishnan"> Anand Balakrishnan</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanjana%20R."> Sanjana R.</a>, <a href="https://publications.waset.org/abstracts/search?q=Solomon%20F."> Solomon F.</a>, <a href="https://publications.waset.org/abstracts/search?q=Vijayalakshmi%20J."> Vijayalakshmi J.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: There is evidence that male factors contribute to the infertility of up to 50% of couples, who are evaluated and treated for infertility using advanced assisted reproductive technologies. Genetic abnormalities, including sperm chromosome aneuploidy as well as structural aberrations, are one of the major causes of male infertility. Recent advances in technology expedite the evaluation of sperm aneuploidy. The purpose of the study was to de-termine the prevalence of sperm aneuploidy in infertile males and the degree of association between DNA fragmentation and sperm aneuploidy. Methods: In this study, 75 infertile men were included, and they were divided into four abnormal groups (Oligospermia, Terato-spermia, Asthenospermia and Oligoasthenoteratospermia (OAT)). Men with children who were normozoospermia served as the control group. The Fluorescence in situ hybridization (FISH) method was used to test for sperm aneuploidy, and the Sperm Chromatin Dispersion Assay (SCDA) was used to measure the fragmentation of sperm DNA. Spearman's correla-tion coefficient was used to evaluate the relationship between sperm aneuploidy and sperm DNA fragmentation along with age. P < 0.05 was regarded as significant. Results: 75 partic-ipants' ages varied from 28 to 48 years old (35.5±5.1). The percentage of spermatozoa bear-ing X and Y was determined to be statistically significant (p-value < 0.05) and was found to be 48.92% and 51.18% of CEP X X 1 – nucish (CEP XX 1) [100] and CEP Y X 1 – nucish (CEP Y X 1) [100]. When compared to the rate of DNA fragmentation, it was discovered that infertile males had a greater frequency of sperm aneuploidy. Asthenospermia and OAT groups in sex chromosomal aneuploidy were significantly correlated (p<0.05). Conclusion: Sperm FISH and SCDA assay results showed increased sperm aneuploidy frequency, and DNA fragmentation index in infertile men compared with fertile men. There is a significant relationship observed between sperm aneuploidy and DNA fragmentation in OAT patients. When evaluating male variables and idiopathic infertility, the sperm FISH screening method can be used as a valuable diagnostic tool. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ale%20infertility" title="ale infertility">ale infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=dfi%20%28dna%20fragmentation%20assay%29%20%28scd-sperm%20chromatin%20dispersion%29.art%20%28artificial%20reproductive%20technology%29" title=" dfi (dna fragmentation assay) (scd-sperm chromatin dispersion).art (artificial reproductive technology)"> dfi (dna fragmentation assay) (scd-sperm chromatin dispersion).art (artificial reproductive technology)</a>, <a href="https://publications.waset.org/abstracts/search?q=trisomy" title=" trisomy"> trisomy</a>, <a href="https://publications.waset.org/abstracts/search?q=aneuploidy" title=" aneuploidy"> aneuploidy</a>, <a href="https://publications.waset.org/abstracts/search?q=fish%20%28fluorescence%20in-situ%20hybridization%29" title=" fish (fluorescence in-situ hybridization)"> fish (fluorescence in-situ hybridization)</a>, <a href="https://publications.waset.org/abstracts/search?q=oat%20%28oligoasthoteratospermia%29" title=" oat (oligoasthoteratospermia)"> oat (oligoasthoteratospermia)</a> </p> <a href="https://publications.waset.org/abstracts/176566/assessment-of-sperm-aneuploidy-using-advanced-sperm-fish-technique-in-infertile-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/176566.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">54</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">764</span> Collection, Cryopreservation, and Fertilizing Potential of Bovine Spermatozoa Collected from the Epididymis Evaluated by Conventional Techniques and by Flow Cytometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20H.%20Moreira%20da%20Silva">M. H. Moreira da Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Valadao"> L. Valadao</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Moreira%20da%20Silva"> F. Moreira da Silva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present study, the fertilizing capacity of bovine spermatozoa was evaluated before and after its cryopreservation. For this, the testicles of 100 bulls slaughtered on Terceira Island were dissected, the epididymal tails were separated, and semen was recovered by the flotation method and then evaluated by phase contrast microscopy and by flow cytometry. For phase contrast microscopy, a drop of semen was used to evaluate the percentage of motile spermatozoa (from 0 to 100%) and motility (from 0 to 5). After determining the concentration and the abnormal forms, semen was diluted to a final concentration of 50 x 106 spz/ml and evaluated by flow cytometer for membrane and acrosome integrity using the conjugation of fluorescent probes propidium iodide (PI) and Arachis hypogea agglutinin (FITC-PNA). Freezing was carried out in a programmable semen freezer, using 0.25 ml straws, in a total of 20 x 106 viable sperm per straw with glycerol as a cryoprotectant in a final concentration of 0.58 M. It was observed that, on average, a total of 7.25 ml of semen was collected from each bull. The viability and vitality rates were respectively 83.22 ± 7.52% and 3.8 ± 0.4 before freezing, decreasing to 58.81 ± 11.99% and 3.6 ± 0.6, respectively, after thawing. Regarding cytoplasmic droplets, it was observed that a high percentage of spermatozoa had medial cytoplasmic droplets (38.47%), with only 3.32% and 0.15% presenting proximal and distal cytoplasmic drops, respectively. By flow cytometry, it was observed that before freezing, the percentage of sperm with the damaged plasma membrane and intact acrosome was 3.61 ± 0.99%, increasing slightly to 4.21 ± 1.86% after cryopreservation (p<0.05). Regarding spermatozoa with damaged plasma membrane and acrosome, the percentage before freezing was 3.37±1.87%, increasing to 4.34 ±1.16% after thawing, and no significant differences were observed between these two values. For the percentage of sperm with the intact plasma membrane and damaged acrosome, this value was 2.04 ± 2.34% before freezing, decreasing to 0.89 ± 0.48% after thawing (p<0.05). The percentage of sperm with the intact plasma membrane and acrosome before freezing was 90.99±2.75%, with a slight decrease to 90.57±3.15% after thawing (p<0.05). From this study, it can be clearly concluded that, after the slaughtering of bulls, the spermatozoa can be recovered from the epididymis and cryopreserved, maintaining an excellent rate of sperm viability and quality after thawing. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bovine%20semen" title="bovine semen">bovine semen</a>, <a href="https://publications.waset.org/abstracts/search?q=epididymis" title=" epididymis"> epididymis</a>, <a href="https://publications.waset.org/abstracts/search?q=cryopreservation" title=" cryopreservation"> cryopreservation</a>, <a href="https://publications.waset.org/abstracts/search?q=fertility%20assessment" title=" fertility assessment"> fertility assessment</a> </p> <a href="https://publications.waset.org/abstracts/158029/collection-cryopreservation-and-fertilizing-potential-of-bovine-spermatozoa-collected-from-the-epididymis-evaluated-by-conventional-techniques-and-by-flow-cytometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/158029.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">89</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">763</span> Effects of Injection of eCG and Oxytocin on Semen Characteristics of Zel Rams in Nonbreeding Season</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Khosro%20Ghazvinian">Khosro Ghazvinian</a>, <a href="https://publications.waset.org/abstracts/search?q=Reza%20Narenji%20Sani"> Reza Narenji Sani</a>, <a href="https://publications.waset.org/abstracts/search?q=Touba%20Khodaiean"> Touba Khodaiean</a>, <a href="https://publications.waset.org/abstracts/search?q=Melika%20Moezifar"> Melika Moezifar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many previous studies have reported that eCG was effective for completing spermatogenesis. In mice, eCG increased testes weight. In addition, Oxytocin (OT) was important in sperm transition and sperm motility in domestic animals. Peripheral circulation of OT also, was increased during sex incitement and ejaculation The objective of this study was to investigate the effect of IM injection of eCG and OT on semen characteristics in Zel rams in out of breeding season. Eighteen 3-year-old Zel adult rams were randomly divided into five equal groups (control and four treatment groups). 0.9% NaCl (1 ml) was injected IM into each ram in the control group, whereas eCG was administered IM at a single dose of 400 IU and 600 IU to each ram in the two eCG treatment groups and OT was administered IM at a single dose of 5 IU and 10 IU to each ram in the other two OT treatment groups. Semen samples were taken by an electroejaculator from all rams 10 min after the IM injection of 0.9% NaCl, eCG, or OT. eCG did not alter semen volume, and OT did not alter sperm motility or abnormal sperm, in comparison to the control values. Mass activity, sperm motility and total sperm number increased significantly in eCG group compared to the control group; and semen volume, mass activity, total sperm number of the OT treatment groups increased significantly compared to the control group. Exogenous 600 IU eCG and 10 IU OT increase mass activity, total sperm number, lived sperm and sperm concentration in Zel rams. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=eCG" title="eCG">eCG</a>, <a href="https://publications.waset.org/abstracts/search?q=oxytocine" title=" oxytocine"> oxytocine</a>, <a href="https://publications.waset.org/abstracts/search?q=semen%20characteristics" title=" semen characteristics"> semen characteristics</a>, <a href="https://publications.waset.org/abstracts/search?q=Zel%20Ram" title=" Zel Ram"> Zel Ram</a>, <a href="https://publications.waset.org/abstracts/search?q=nonbreeding%20season" title=" nonbreeding season"> nonbreeding season</a> </p> <a href="https://publications.waset.org/abstracts/47108/effects-of-injection-of-ecg-and-oxytocin-on-semen-characteristics-of-zel-rams-in-nonbreeding-season" class="btn btn-primary btn-sm">Procedia</a> <a 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