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class="breadcrumb_last" aria-current="page">Lyophilization in the pharmaceutical industry</span></span></p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-2964b189 elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="2964b189" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-4742de37" data-id="4742de37" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-6d6cb80d elementor-widget elementor-widget-theme-post-featured-image elementor-widget-image" data-id="6d6cb80d" data-element_type="widget" data-widget_type="theme-post-featured-image.default"> <div class="elementor-widget-container"> <img fetchpriority="high" width="512" height="328" src="https://demaco-cryogenics.com/wp-content/uploads/2023/02/lyophilized.png" class="attachment-full size-full wp-image-50057" alt="lyophilized" srcset="https://demaco-cryogenics.com/wp-content/uploads/2023/02/lyophilized.png 512w, https://demaco-cryogenics.com/wp-content/uploads/2023/02/lyophilized-300x192.png 300w" sizes="(max-width: 512px) 100vw, 512px" /> </div> </div> <div class="elementor-element elementor-element-5c9a9c8 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading" data-id="5c9a9c8" data-element_type="widget" data-widget_type="theme-post-title.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Lyophilization in the pharmaceutical industry</h2> </div> </div> <div class="elementor-element elementor-element-c5cd23e elementor-widget elementor-widget-theme-post-content" data-id="c5cd23e" data-element_type="widget" data-widget_type="theme-post-content.default"> <div class="elementor-widget-container"> <div data-elementor-type="wp-post" data-elementor-id="50046" class="elementor elementor-50046" data-elementor-post-type="blog"> <section class="elementor-section elementor-top-section elementor-element elementor-element-77c001f elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="77c001f" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1e3fff8" data-id="1e3fff8" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-4bf1a27 elementor-widget elementor-widget-text-editor" data-id="4bf1a27" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p><em>Lyophilization</em>, also known as freeze-drying, plays an essential role in the pharmaceutical industry. This industry has been using freeze-drying techniques for many years to extend the shelf life of various medicines.<br />How exactly does <em>lyophilization</em> work? What does a lyophilizer do, and why is this <a href="/cryogenics/cryogenic-applications/"><em>cryogenic application</em></a> so valuable for the pharmaceutical industry? Read on for detailed answers to these questions.</p><h2>What is lyophilization?</h2><p><em>Lyophilization</em> takes place in a freeze dryer. In this (often) <a href="https://demaco-cryogenics.com/blog/cryogenic-freezer/"><em>cryogenic freezer</em></a>, a product is first brought to a low temperature, after which almost all the moisture is removed from it.<br />In most cases, this process consists of three steps:</p><h3>1. Freezing the product.</h3><p>Lyophilization begins with freezing a product at a temperature of around -40掳C to -50掳C at atmospheric pressure. Liquid nitrogen is often used during this phase as a fast, environmentally friendly, and affordable refrigerant. Rapid freezing can be essential when freeze-drying substances that are sensitive to crystal formation.<br />The product is cooled to below the triple point during this first stage. This ensures that sublimation, rather than melting, occurs in the primary drying phase.</p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-0f23270 elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="0f23270" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b45e638" data-id="b45e638" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-c26f752 elementor-widget elementor-widget-image" data-id="c26f752" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img fetchpriority="high" fetchpriority="high" decoding="async" width="512" height="445" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%20512%20445'%3E%3C/svg%3E" class="attachment-large size-large wp-image-50062" alt="water phase diagram" data-lazy-srcset="https://demaco-cryogenics.com/wp-content/uploads/2023/02/water-phase-diagram.jpg 512w, https://demaco-cryogenics.com/wp-content/uploads/2023/02/water-phase-diagram-300x261.jpg 300w" data-lazy-sizes="(max-width: 512px) 100vw, 512px" data-lazy-src="https://demaco-cryogenics.com/wp-content/uploads/2023/02/water-phase-diagram.jpg" /><noscript><img fetchpriority="high" fetchpriority="high" decoding="async" width="512" height="445" src="https://demaco-cryogenics.com/wp-content/uploads/2023/02/water-phase-diagram.jpg" class="attachment-large size-large wp-image-50062" alt="water phase diagram" srcset="https://demaco-cryogenics.com/wp-content/uploads/2023/02/water-phase-diagram.jpg 512w, https://demaco-cryogenics.com/wp-content/uploads/2023/02/water-phase-diagram-300x261.jpg 300w" sizes="(max-width: 512px) 100vw, 512px" /></noscript> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-81a39f4 elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="81a39f4" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b389566" data-id="b389566" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-d78880a elementor-widget elementor-widget-text-editor" data-id="d78880a" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Source: <a href="https://naeye.net/15503/science/triple-point-of-water-the-temperature-where-all-three-phases-coexist/" target="_blank" rel="noopener">Na Eye</a><br>The triple point of water</p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-a31d06c elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="a31d06c" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1012f30" data-id="1012f30" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-7f96806 elementor-widget elementor-widget-text-editor" data-id="7f96806" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <h3>2. Primary drying phase (sublimation).</h3><p>Next, the cooled product is slightly heated at a reduced pressure, which causes the sublimation, as mentioned earlier, to take place. A vacuum is also created in the freeze dryer, further accelerating the sublimation process.<br />During this phase, the formed water vapors are accumulated by a condenser, which causes the vapors to take on a solid form.</p><p>The vast majority of moisture is removed from the product during the primary drying phase, while its structure remains optimally intact.</p><h3>3. Secondary drying phase (absorption)</h3><p>In the final stage, secondary drying, known as absorption, takes place. The ionically bonded water molecules are extracted from the product during this phase.<br />During this phase, the temperature rises slightly, sometimes even above 0 掳C. The vacuum is then breached using an inert gas, such as nitrogen, after which the product is sealed.<br />On completing these three steps, the product has a porous shape and often contains no more than 1-4% water.</p><h3><em>Lyophilization</em> in the pharmaceutical industry</h3><p><em>Lyophilization</em> is mainly used in the food industry for food preservation and in the pharmaceutical industry to produce and preserve (mainly biopharmaceutical) pharmaceuticals.<br />Many of these pharmaceuticals contain complex molecular structures, which are unstable in liquid form. Therefore, if these medicines are stored undried, their shelf life is limited.<br />When the moisture is removed from the structures, they stabilize considerably, allowing the medication to be stored for an extended period and reconstituted later by adding moisture again.<br />Examples of pharmaceutical materials treated using<em> lyophilization</em> are antibiotics, vaccines, bacteria, and diagnostic drugs.</p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-8b68f1b elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="8b68f1b" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-145a604" data-id="145a604" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-e610802 elementor-widget elementor-widget-image" data-id="e610802" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img decoding="async" width="512" height="288" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%20512%20288'%3E%3C/svg%3E" class="attachment-large size-large wp-image-50047" alt="diagram of a lyophilizer" data-lazy-srcset="https://demaco-cryogenics.com/wp-content/uploads/2023/02/diagram-of-a-lyophilizer.png 512w, https://demaco-cryogenics.com/wp-content/uploads/2023/02/diagram-of-a-lyophilizer-300x169.png 300w" data-lazy-sizes="(max-width: 512px) 100vw, 512px" data-lazy-src="https://demaco-cryogenics.com/wp-content/uploads/2023/02/diagram-of-a-lyophilizer.png" /><noscript><img decoding="async" width="512" height="288" src="https://demaco-cryogenics.com/wp-content/uploads/2023/02/diagram-of-a-lyophilizer.png" class="attachment-large size-large wp-image-50047" alt="diagram of a lyophilizer" srcset="https://demaco-cryogenics.com/wp-content/uploads/2023/02/diagram-of-a-lyophilizer.png 512w, https://demaco-cryogenics.com/wp-content/uploads/2023/02/diagram-of-a-lyophilizer-300x169.png 300w" sizes="(max-width: 512px) 100vw, 512px" /></noscript> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-6d85002 elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="6d85002" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b1fe174" data-id="b1fe174" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-59f615c elementor-widget elementor-widget-text-editor" data-id="59f615c" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>The above diagram of <a href="https://lubrizolcdmo.com/blog/lyophilization-of-pharmaceuticals-an-overview/#:~:text=Lyophilization%20transforms%20a%20drug%20product,small%20and%20large%20molecule%20drugs." target="_blank" rel="noopener">Lubrizol CDMO</a> clearly shows the process of lyophilization in the pharmaceutical industry. The vials containing the drug are cooled and dried by using a condenser. A vacuum is also created in the application to speed up the sublimation process.</p><h2><br />Challenges with <em>lyophilization</em></h2><p>Lyophilization is a complex process involving several challenges. For example, the composition of each substance or drug is different, and how to perform the lyophilization must be examined for each material. All aspects and components of the process must be perfectly matched to the specific material to avoid damage. This research requires a lot of time and expertise.<br />During lyophilization, products are exposed to the environment for a considerable time, creating contamination risk. Sterile lyophilization is therefore extremely important, and freeze-dryers for drugs are subject to stringent safety requirements. Large-scale use of lyophilization is therefore costly.</p><h2><br /><em>Lyophilization</em> and cryogenics</h2><p>So, what is the role of cryogenics in lyophilization?<br />Freeze dryers can be found in many different forms. Small, large, and with different cooling systems. Mechanical or cryogenic. In the pharmaceutical industry, many freeze-dryers use liquid nitrogen for the first stage of the freeze-drying process: freezing the product.<br />Cryogenic freeze dryers offer several <a href="https://www.spscientific.com/DownloadFile.aspx?fileid=2662&filename=Advances" target="_blank" rel="noopener">advantages</a>, including:</p><ul><li>Low temperatures are <strong>easier</strong> achieved than with mechanical refrigeration. This is because liquid nitrogen has a temperature as low as – 196 掳C.</li><li>Cooling of the products is accomplished <strong>quicker</strong>, often a prerequisite for the lyophilization of vaccines.</li><li>The system does not require refrigerants that are harmful to the <strong>environment.</strong></li><li>Compared to mechanical systems, the <strong>cost</strong> of cooling using liquid nitrogen is often lower.</li><li>A cryogenic cooling system requires <strong>less maintenance,</strong> again reducing the overall cost of the system.</li><li>A cryogenic cooling system is usually <strong>quieter</strong> than a mechanical system.</li></ul><p>For these reasons, cryogenic lyophilization systems are prevalent in the pharmaceutical industry. Hence Demaco often works together with this industry and supplies the necessary infrastructures to make cryogenic <em>lyophilization</em> possible. </p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-80089ff elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="80089ff" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f02c099" data-id="f02c099" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-7f6b9c9 elementor-widget elementor-widget-image" data-id="7f6b9c9" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img decoding="async" width="512" height="328" src="https://demaco-cryogenics.com/wp-content/uploads/2023/02/lyophilized.png" class="attachment-large size-large wp-image-50057" alt="lyophilized" srcset="https://demaco-cryogenics.com/wp-content/uploads/2023/02/lyophilized.png 512w, https://demaco-cryogenics.com/wp-content/uploads/2023/02/lyophilized-300x192.png 300w" sizes="(max-width: 512px) 100vw, 512px" /> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-e367b31 elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="e367b31" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5066b7e" data-id="5066b7e" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-ee12a78 elementor-widget elementor-widget-text-editor" data-id="ee12a78" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <h2>Demaco’s infrastructures for the pharmaceutical industry</h2> <p>For a cryogenic freeze dryer to function, cryogenic liquid is required. Which, in most cases, as outlined above, is liquid nitrogen.</p> <p>Companies in the pharmaceutical industry that utilize cryogenic <em>lyophilization</em> have one or more cryogenic tanks, also known as <a href="/blog/cryogenic-storage-dewar/">dewars</a>, located inside or outside their building. These dewars contain the required liquid nitrogen.</p> <p>As an expert in cryogenic infrastructures, Demaco ensures that the liquid nitrogen reaches the freeze dryer at the proper pressure from these storage tanks while in optimum quality.</p> <p>In cooperation with the customer, our experts evaluate the requirements for a specific cryogenic application. We will also provide the proper <a href="https://demaco-cryogenics.com/products/vacuum-insulated-transfer-lines/">vacuum insulated transfer lines</a> (VIP), the proper couplings, and any additional <a href="https://demaco-cryogenics.com/products/">quality improvement products</a>.</p> <p>With years of experience in the pharmaceutical industry, we know exactly what a perfect infrastructure for a cryogenic freeze dryer needs.</p> <h2>Would you like to know more?</h2> <p>Do you have questions about the installation of a freeze dryer? Or would you like to know more about Demaco’s products and services? Feel free to <a href="https://demaco-cryogenics.com/contact/">contact</a> us or take a look at our <a href="https://demaco-cryogenics.com/products/">products</a> and <a href="https://demaco-cryogenics.com/projects/">projects</a>.</p> </div> </div> </div> </div> </div> </section> </div> </div> </div> <section class="elementor-section elementor-inner-section elementor-element elementor-element-1812088 elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="1812088" data-element_type="section"> <div class="elementor-container elementor-column-gap-default"> <div class="elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-89a5695" data-id="89a5695" data-element_type="column"> <div class="elementor-widget-wrap"> </div> </div> <div class="elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-81c0342" data-id="81c0342" data-element_type="column"> <div 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Noord-Scharwoude <br />The Netherlands</p><p><span style="color: #ffffff;"><a style="color: #ffffff;" href="tel:+31 226 33 21 00">T: +31 226 33 21 00</a></span><br /><span style="color: #ffffff;"><a style="color: #ffffff;" href="mailto:info@demaco-cryogenics.com">E: info@demaco-cryogenics.com</a></span></p> </div> </div> <div class="elementor-element elementor-element-f890a3e elementor-widget elementor-widget-text-editor" data-id="f890a3e" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p><strong>Post address</strong><br />PO Box 4<br />1723 ZG, Noord-Scharwoude <br />The Netherlands</p> </div> </div> </div> </div> <div class="elementor-column elementor-col-25 elementor-top-column elementor-element elementor-element-2cd42ec" data-id="2cd42ec" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-c7b2d2c elementor-widget 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data-popmake="{"id":46863,"slug":"product-sheet-download","theme_id":46836,"cookies":[],"triggers":[{"type":"click_open","settings":{"cookie_name":"","extra_selectors":".download-product-sheet"}}],"mobile_disabled":null,"tablet_disabled":null,"meta":{"display":{"stackable":false,"overlay_disabled":false,"scrollable_content":false,"disable_reposition":false,"size":"medium","responsive_min_width":"0%","responsive_min_width_unit":false,"responsive_max_width":"640px","responsive_max_width_unit":false,"custom_width":"640px","custom_width_unit":false,"custom_height":"380px","custom_height_unit":false,"custom_height_auto":false,"location":"center top","position_from_trigger":false,"position_top":"100","position_left":"0","position_bottom":"0","position_right":"0","position_fixed":false,"animation_type":"fade","animation_speed":"350","animation_origin":"center top","overlay_zindex":false,"zindex":"1999999999"},"close":{"text":"","button_delay":"0","overlay_click":false,"esc_press":false,"f4_press":false},"click_open":[]}}"> <div id="popmake-46863" class="pum-container popmake theme-46836 pum-responsive pum-responsive-medium responsive size-medium"> <div id="pum_popup_title_46863" class="pum-title popmake-title"> Receive product sheets </div> <div class="pum-content popmake-content" tabindex="0"> <p>Already a customer of Demaco Holland B.V.? You already have access to the linked files but feel free to submit a new request.<br /> <div class='gf_browser_chrome gform_wrapper gravity-theme gform-theme--no-framework' data-form-theme='gravity-theme' data-form-index='0' id='gform_wrapper_16' ><div id='gf_16' class='gform_anchor' tabindex='-1'></div> <div class='gform_heading'> <p class='gform_required_legend'>"<span class="gfield_required gfield_required_asterisk">*</span>" indicates required fields</p> </div><form method='post' enctype='multipart/form-data' target='gform_ajax_frame_16' id='gform_16' action='/blog/lyophilization-in-the-pharmaceutical-industry-2/#gf_16' data-formid='16' novalidate> <input type='hidden' class='gforms-pum' value='{"closepopup":false,"closedelay":0,"openpopup":false,"openpopup_id":0}' /> <div class='gform-body gform_body'><div id='gform_fields_16' class='gform_fields top_label form_sublabel_below description_below validation_below'><fieldset id="field_16_1" class="gfield gfield--type-name 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You already have access to the linked files but feel free to submit a new request.<br /> <div class='gf_browser_chrome gform_wrapper gravity-theme gform-theme--no-framework' data-form-theme='gravity-theme' data-form-index='0' id='gform_wrapper_27' ><div id='gf_27' class='gform_anchor' tabindex='-1'></div> <div class='gform_heading'> <p class='gform_required_legend'>"<span class="gfield_required gfield_required_asterisk">*</span>" indicates required fields</p> </div><form method='post' enctype='multipart/form-data' target='gform_ajax_frame_27' id='gform_27' action='/blog/lyophilization-in-the-pharmaceutical-industry-2/#gf_27' data-formid='27' novalidate> <input type='hidden' class='gforms-pum' value='{"closepopup":false,"closedelay":0,"openpopup":false,"openpopup_id":0}' /> <div class='gform-body gform_body'><div id='gform_fields_27' class='gform_fields top_label form_sublabel_below description_below validation_below'><fieldset id="field_27_1" class="gfield gfield--type-name 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class="gfield gfield--type-checkbox gfield--type-choice gfield_contains_required field_sublabel_below gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_27_4" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >Select product sheet<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></legend><div class='ginput_container ginput_container_checkbox'><div class='gfield_checkbox gfield_choice--select_all_enabled' id='input_27_4'><div class='gchoice gchoice_27_4_1'> <input class='gfield-choice-input' name='input_4.1' type='checkbox' value='VacuumInsulation' id='choice_27_4_1' tabindex='55' /> <label for='choice_27_4_1' id='label_27_4_1' class='gform-field-label gform-field-label--type-inline'>The five advantages of vacuum insulation</label> </div><div class='gchoice gchoice_27_4_2'> <input class='gfield-choice-input' name='input_4.2' type='checkbox' 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type='hidden' class='gform_hidden' name='gform_theme' data-js='gform_theme_27' id='gform_theme_27' value='gravity-theme' /> <input type='hidden' class='gform_hidden' name='gform_style_settings' data-js='gform_style_settings_27' id='gform_style_settings_27' value='' /> <input type='hidden' class='gform_hidden' name='is_submit_27' value='1' /> <input type='hidden' class='gform_hidden' name='gform_submit' value='27' /> <input type='hidden' class='gform_hidden' name='gform_unique_id' value='' /> <input type='hidden' class='gform_hidden' name='state_27' value='WyJbXSIsImIzZGQxOWJiNmVlNjlhMTM4OWM0ZDYzMzZjMmU5ODMzIl0=' /> <input type='hidden' autocomplete='off' class='gform_hidden' name='gform_target_page_number_27' id='gform_target_page_number_27' value='0' /> <input type='hidden' autocomplete='off' class='gform_hidden' name='gform_source_page_number_27' id='gform_source_page_number_27' value='1' /> <input type='hidden' name='gform_field_values' value='' /> </div> </form> </div> <iframe 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100;if(is_form){jQuery('#gform_wrapper_27').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_27').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_27').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ jQuery(document).scrollTop(jQuery('#gform_wrapper_27').offset().top - mt); }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_27').val();gformInitSpinner( 27, 'https://demaco-cryogenics.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [27, current_page]);window['gf_submitting_27'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = 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postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [27, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 27, currentPage: current_page } } ); gform.utils.trigger( { event: 'gform/post_render', native: false, data: { formId: 27, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } );} ); </script> </p> </div> <button type="button" class="pum-close popmake-close" aria-label="Close"> X </button> </div> </div> <div id="pum-54434" role="dialog" aria-modal="false" class="pum pum-overlay pum-theme-46836 pum-theme-default-theme popmake-overlay click_open" data-popmake="{"id":54434,"slug":"download-lng","theme_id":46836,"cookies":[{"event":"on_popup_close","settings":{"name":"pum-54434","key":"","session":false,"path":"1","time":"1 month"}}],"triggers":[{"type":"click_open","settings":{"extra_selectors":""}}],"mobile_disabled":null,"tablet_disabled":null,"meta":{"display":{"stackable":false,"overlay_disabled":false,"scrollable_content":false,"disable_reposition":false,"size":"medium","responsive_min_width":"0%","responsive_min_width_unit":false,"responsive_max_width":"100%","responsive_max_width_unit":false,"custom_width":"640px","custom_width_unit":false,"custom_height":"380px","custom_height_unit":false,"custom_height_auto":false,"location":"center top","position_from_trigger":false,"position_top":"100","position_left":"0","position_bottom":"0","position_right":"0","position_fixed":false,"animation_type":"fade","animation_speed":"350","animation_origin":"center top","overlay_zindex":false,"zindex":"1999999999"},"close":{"text":"","button_delay":"0","overlay_click":false,"esc_press":false,"f4_press":false},"click_open":[]}}"> <div id="popmake-54434" class="pum-container popmake theme-46836 pum-responsive pum-responsive-medium responsive size-medium"> <div class="pum-content popmake-content" tabindex="0"> <p>Already a customer of Demaco Holland B.V.? You already have access to the linked files but feel free to submit a new request.<br /> </p> </div> <button type="button" class="pum-close popmake-close" aria-label="Close"> X </button> </div> </div> <div id="pum-48865" role="dialog" aria-modal="false" aria-labelledby="pum_popup_title_48865" class="pum pum-overlay pum-theme-46836 pum-theme-default-theme popmake-overlay click_open" data-popmake="{"id":48865,"slug":"watch-webinar-2","theme_id":46836,"cookies":[],"triggers":[{"type":"click_open","settings":{"extra_selectors":""}}],"mobile_disabled":null,"tablet_disabled":null,"meta":{"display":{"stackable":false,"overlay_disabled":false,"scrollable_content":false,"disable_reposition":false,"size":"medium","responsive_min_width":"0%","responsive_min_width_unit":false,"responsive_max_width":"100%","responsive_max_width_unit":false,"custom_width":"640px","custom_width_unit":false,"custom_height":"380px","custom_height_unit":false,"custom_height_auto":false,"location":"center top","position_from_trigger":false,"position_top":"100","position_left":"0","position_bottom":"0","position_right":"0","position_fixed":false,"animation_type":"fade","animation_speed":"350","animation_origin":"center top","overlay_zindex":false,"zindex":"1999999999"},"close":{"text":"","button_delay":"0","overlay_click":false,"esc_press":false,"f4_press":false},"click_open":[]}}"> <div id="popmake-48865" class="pum-container popmake theme-46836 pum-responsive pum-responsive-medium responsive size-medium"> <div id="pum_popup_title_48865" class="pum-title popmake-title"> Watch webinar </div> <div class="pum-content popmake-content" tabindex="0"> <div class='gf_browser_chrome gform_wrapper gravity-theme gform-theme--no-framework' data-form-theme='gravity-theme' data-form-index='0' id='gform_wrapper_25' ><div id='gf_25' class='gform_anchor' tabindex='-1'></div><form method='post' enctype='multipart/form-data' target='gform_ajax_frame_25' id='gform_25' action='/blog/lyophilization-in-the-pharmaceutical-industry-2/#gf_25' data-formid='25' novalidate> <input type='hidden' class='gforms-pum' value='{"closepopup":false,"closedelay":0,"openpopup":false,"openpopup_id":0}' /> <div class='gform-body gform_body'><div id='gform_fields_25' class='gform_fields top_label form_sublabel_below description_below validation_below'><fieldset id="field_25_1" class="gfield gfield--type-name gfield_contains_required field_sublabel_above gfield--no-description field_description_below hidden_label field_validation_below gfield_visibility_visible" data-js-reload="field_25_1" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >Name<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_complex ginput_container ginput_container--name no_prefix has_first_name no_middle_name has_last_name no_suffix gf_name_has_2 ginput_container_name gform-grid-row' 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gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_email'> <input name='input_2' id='input_25_2' type='email' value='' class='large' tabindex='54' aria-required="true" aria-invalid="false" /> </div></div><fieldset id="field_25_6" class="gfield gfield--type-checkbox gfield--type-choice gfield--width-full gfield_contains_required field_sublabel_below gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_25_6" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >Select webinar<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_container ginput_container_checkbox'><div class='gfield_checkbox ' id='input_25_6'><div class='gchoice gchoice_25_6_1'> <input class='gfield-choice-input' name='input_6.1' type='checkbox' value='The fundamentals of the liquid hydrogen supply chain' 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