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Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="42380325" href="https://www.academia.edu/Documents/in/Roger_Penrose"><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://independent.academia.edu/AndrzejMackiewicz","location":"/AndrzejMackiewicz","scheme":"https","host":"independent.academia.edu","port":null,"pathname":"/AndrzejMackiewicz","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Roger Penrose"]}" data-trace="false" 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data-dom-id="Pill-react-component-df4430ef-bfc3-4db2-9ec8-e11c033d17a2"></div> <div id="Pill-react-component-df4430ef-bfc3-4db2-9ec8-e11c033d17a2"></div> </a></div></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Andrzej Mackiewicz</h3></div><div class="js-work-strip profile--work_container" data-work-id="38621338"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/38621338/Novel_BRCA1_mutations_and_more_frequent_intron_20_alteration_found_among_236_women_from_Western_Poland"><img alt="Research paper thumbnail of Novel BRCA1 mutations and more frequent intron-20 alteration found among 236 women from Western Poland" class="work-thumbnail" src="https://attachments.academia-assets.com/58698748/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/38621338/Novel_BRCA1_mutations_and_more_frequent_intron_20_alteration_found_among_236_women_from_Western_Poland">Novel BRCA1 mutations and more frequent intron-20 alteration found among 236 women from Western Poland</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AndrzejMackiewicz">Andrzej Mackiewicz</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://torun-pl.academia.edu/MarcinWo%C5%BAniak">Marcin Wo藕niak</a></span></div><div class="wp-workCard_item"><span>Oncogene</span><span>, 1997</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d1d288a2f499984f7f9c180b12373e57" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":58698748,"asset_id":38621338,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/58698748/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="32623469"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/32623469/EPR_Study_of_Iron_Ion_Complexes_in_Human_Blood"><img alt="Research paper thumbnail of EPR Study of Iron Ion Complexes in Human Blood" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/32623469/EPR_Study_of_Iron_Ion_Complexes_in_Human_Blood">EPR Study of Iron Ion Complexes in Human Blood</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AndrzejMackiewicz">Andrzej Mackiewicz</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/EwaLeporowska">Ewa Leporowska</a></span></div><div class="wp-workCard_item"><span>Applied Magnetic Resonance</span><span>, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Electronic states of iron ion complexes in human blood from patients with melanoma have been inve...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Electronic states of iron ion complexes in human blood from patients with melanoma have been investigated by electron paramagnetic resonance (EPR). The measurements were performed at liquid nitrogen temperature (77聽K) on an X-band EPR spectrometer. Numerous types of iron paramagnetic centers have been identified. In several kinds of protein complexes exemplified by methemoglobin, transferrin or ferritin, various forms of trivalent iron have been found. Three groups of patients with typical EPR spectra have been individualized. These groups differed in types and concentration of paramagnetic centers in peripheral blood. A good correlation has been found between the EPR results, the total iron ion complexes concentration and transferrin saturation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="32623469"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="32623469"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32623469; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32623469]").text(description); $(".js-view-count[data-work-id=32623469]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32623469; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='32623469']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 32623469, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=32623469]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":32623469,"title":"EPR Study of Iron Ion Complexes in Human Blood","translated_title":"","metadata":{"abstract":"Electronic states of iron ion complexes in human blood from patients with melanoma have been investigated by electron paramagnetic resonance (EPR). 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A good correlation has been found between the EPR results, the total iron ion complexes concentration and transferrin saturation.","internal_url":"https://www.academia.edu/32623469/EPR_Study_of_Iron_Ion_Complexes_in_Human_Blood","translated_internal_url":"","created_at":"2017-04-24T12:13:56.296-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42799428,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":28636513,"work_id":32623469,"tagging_user_id":42799428,"tagged_user_id":42380325,"co_author_invite_id":null,"email":"a***c@amu.edu.pl","display_order":0,"name":"Andrzej Mackiewicz","title":"EPR Study of Iron Ion Complexes in Human Blood"}],"downloadable_attachments":[],"slug":"EPR_Study_of_Iron_Ion_Complexes_in_Human_Blood","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":42799428,"first_name":"Ewa","middle_initials":"","last_name":"Leporowska","page_name":"EwaLeporowska","domain_name":"independent","created_at":"2016-02-07T04:44:10.817-08:00","display_name":"Ewa Leporowska","url":"https://independent.academia.edu/EwaLeporowska"},"attachments":[],"research_interests":[{"id":116193,"name":"Solid State electronic devices","url":"https://www.academia.edu/Documents/in/Solid_State_electronic_devices"},{"id":158597,"name":"Iron","url":"https://www.academia.edu/Documents/in/Iron"},{"id":300832,"name":"Protein Complex Detection","url":"https://www.academia.edu/Documents/in/Protein_Complex_Detection"},{"id":945101,"name":"Liquid Nitrogen","url":"https://www.academia.edu/Documents/in/Liquid_Nitrogen"},{"id":1430522,"name":"Electron Paramagnetic Resonance","url":"https://www.academia.edu/Documents/in/Electron_Paramagnetic_Resonance"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="31510456"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/31510456/TRIM28_multi_domain_protein_regulates_cancer_stem_cell_population_in_breast_tumor_development"><img alt="Research paper thumbnail of TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/31510456/TRIM28_multi_domain_protein_regulates_cancer_stem_cell_population_in_breast_tumor_development">TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JannikNAndersen">Jannik Andersen</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AndrzejMackiewicz">Andrzej Mackiewicz</a></span></div><div class="wp-workCard_item"><span>Oncotarget</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The expression of Tripartite motif-containing protein 28 (TRIM28)/Kr眉ppel-associated box (KRAB)-a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The expression of Tripartite motif-containing protein 28 (TRIM28)/Kr眉ppel-associated box (KRAB)-associated protein 1 (KAP1), is elevated in at least 14 tumor types, including solid and hematopoietic tumors. High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo. Downregulation of TRIM28 expression in xenografts led to deceased expression of pluripotency and mesenchymal markers, as well as inhibition of signaling pathways involved in the complex mechanism of CSC maintenance. Moreover, TRIM28 depletion reduced the ability of cancer cells to induce tumor growth when subcutaneously injected in limiting dilutions. Our data demonstrate that the downregulation of TRIM28 gene expression reduced the ability of CSCs to self-renew that resulted in significant reduction of tumor growth. Loss of function of TRIM28 leads to dysregulation of cell cycle, cellular response to stress, cancer cell metabolism, and inhibition of oxidative phosphorylation. All these mechanisms directly regulate maintenance of CSC population. Our original results revealed the role of the TRIM28 in regulating the CSC population in breast cancer. These findings may pave the way to novel and more effective therapies targeting cancer stem cells in breast tumors.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="31510456"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="31510456"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 31510456; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=31510456]").text(description); $(".js-view-count[data-work-id=31510456]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 31510456; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='31510456']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 31510456, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=31510456]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":31510456,"title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development","translated_title":"","metadata":{"abstract":"The expression of Tripartite motif-containing protein 28 (TRIM28)/Kr眉ppel-associated box (KRAB)-associated protein 1 (KAP1), is elevated in at least 14 tumor types, including solid and hematopoietic tumors. High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo. Downregulation of TRIM28 expression in xenografts led to deceased expression of pluripotency and mesenchymal markers, as well as inhibition of signaling pathways involved in the complex mechanism of CSC maintenance. Moreover, TRIM28 depletion reduced the ability of cancer cells to induce tumor growth when subcutaneously injected in limiting dilutions. Our data demonstrate that the downregulation of TRIM28 gene expression reduced the ability of CSCs to self-renew that resulted in significant reduction of tumor growth. Loss of function of TRIM28 leads to dysregulation of cell cycle, cellular response to stress, cancer cell metabolism, and inhibition of oxidative phosphorylation. All these mechanisms directly regulate maintenance of CSC population. Our original results revealed the role of the TRIM28 in regulating the CSC population in breast cancer. These findings may pave the way to novel and more effective therapies targeting cancer stem cells in breast tumors.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Oncotarget"},"translated_abstract":"The expression of Tripartite motif-containing protein 28 (TRIM28)/Kr眉ppel-associated box (KRAB)-associated protein 1 (KAP1), is elevated in at least 14 tumor types, including solid and hematopoietic tumors. High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo. Downregulation of TRIM28 expression in xenografts led to deceased expression of pluripotency and mesenchymal markers, as well as inhibition of signaling pathways involved in the complex mechanism of CSC maintenance. Moreover, TRIM28 depletion reduced the ability of cancer cells to induce tumor growth when subcutaneously injected in limiting dilutions. Our data demonstrate that the downregulation of TRIM28 gene expression reduced the ability of CSCs to self-renew that resulted in significant reduction of tumor growth. Loss of function of TRIM28 leads to dysregulation of cell cycle, cellular response to stress, cancer cell metabolism, and inhibition of oxidative phosphorylation. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256067"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21256067/Gene_modified_tumor_vaccines_in_therapy_of_malignant_melanoma"><img alt="Research paper thumbnail of Gene modified tumor vaccines in therapy of malignant melanoma" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256067/Gene_modified_tumor_vaccines_in_therapy_of_malignant_melanoma">Gene modified tumor vaccines in therapy of malignant melanoma</a></div><div class="wp-workCard_item"><span>Otolaryngologia polska. The Polish otolaryngology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Over the years the incidence of malignant melanoma in Poland as well as in other countries has be...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Over the years the incidence of malignant melanoma in Poland as well as in other countries has been continuously increasing. Surgery is a treatment of choice in the early stages of primary lesions. Advanced malignant melanoma however is resistant to chemotherapy or radiotherapy. Therefore there is a need for new, more effective treatments. In the last years biotherapy such as immunotherapy is focusing a lot of attention. Unfortunately, systemic administration of immunostimulatory factors is very often associated with severe side effects. Thus, concepts of specific immunotherapies such as immunogene therapy have been developed. Currently, various gene therapy strategies of malignant melanoma are being evaluated in multiple clinical trials carried out all over the world. They include gene modified cancer vaccines (GMTV) modified with genes encoding (i) cytokines or (ii) costimulatory molecules and dendritic cells modified with (iii) genes encoding tumor antigens or (iv) immunostimulatory factors. Since January 1996 in Department of Cancer Immunology USOMS, at GreatPoland Cancer Center in Poznan, Poland a GMTV has been tested in malignant melanoma patients. For the last 6 years more than 220 patients were enrolled into study of GMTV consisting of melanoma cells modified with genes encoding IL-6 and its agonistic soluble receptor (sIL-6R). More than 25% of objective clinical responses and significant life extension were observed. 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In the last years biotherapy such as immunotherapy is focusing a lot of attention. Unfortunately, systemic administration of immunostimulatory factors is very often associated with severe side effects. Thus, concepts of specific immunotherapies such as immunogene therapy have been developed. Currently, various gene therapy strategies of malignant melanoma are being evaluated in multiple clinical trials carried out all over the world. They include gene modified cancer vaccines (GMTV) modified with genes encoding (i) cytokines or (ii) costimulatory molecules and dendritic cells modified with (iii) genes encoding tumor antigens or (iv) immunostimulatory factors. Since January 1996 in Department of Cancer Immunology USOMS, at GreatPoland Cancer Center in Poznan, Poland a GMTV has been tested in malignant melanoma patients. For the last 6 years more than 220 patients were enrolled into study of GMTV consisting of melanoma cells modified with genes encoding IL-6 and its agonistic soluble receptor (sIL-6R). More than 25% of objective clinical responses and significant life extension were observed. 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Ocena efektu przeciwnowotworowego genetycznie modyfikowanej szczepionki kom贸rkowej w mysim modelu raka nerki" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256066/239_Ocena_efektu_przeciwnowotworowego_genetycznie_modyfikowanej_szczepionki_kom%C3%B3rkowej_w_mysim_modelu_raka_nerki">239. Ocena efektu przeciwnowotworowego genetycznie modyfikowanej szczepionki kom贸rkowej w mysim modelu raka nerki</a></div><div class="wp-workCard_item"><span>Reports of Practical Oncology & Radiotherapy</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256066]").text(description); $(".js-view-count[data-work-id=21256066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256066, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256066,"title":"239. Ocena efektu przeciwnowotworowego genetycznie modyfikowanej szczepionki kom贸rkowej w mysim modelu raka nerki","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"Reports of Practical Oncology \u0026 Radiotherapy"},"translated_abstract":null,"internal_url":"https://www.academia.edu/21256066/239_Ocena_efektu_przeciwnowotworowego_genetycznie_modyfikowanej_szczepionki_kom%C3%B3rkowej_w_mysim_modelu_raka_nerki","translated_internal_url":"","created_at":"2016-01-30T11:06:54.139-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260662,"work_id":21256066,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":2938834,"email":"p***k@beck.pl","display_order":0,"name":"Piotr Grabarczyk","title":"239. 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Adv Exp Med Biol. 2001;495:389-92. Improving the retroviral vector (RV) systems for immunother...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">1. Adv Exp Med Biol. 2001;495:389-92. Improving the retroviral vector (RV) systems for immunotherapy of cancer. Grabarczyk P, Gryska K, Wysocki PJ, Izycki D, Mackiewicz A. 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IL-6 and GM-CSF in tumor rejection model of renal cell cancer PIOTR J. WYSOCKI*, DARIUS...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Page 399. IL-6 and GM-CSF in tumor rejection model of renal cell cancer PIOTR J. WYSOCKI*, DARIUSZ W. 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The GS-NS0 expres- sion system takes advantage of the strong human cytomegalovirus immediate early promoter ex- pression vector and glutamine synthetase</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="990b479bceee49f44499cea2294433f0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784684,"asset_id":21256063,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784684/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256063"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256063"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256063; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256063]").text(description); $(".js-view-count[data-work-id=21256063]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256063; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256063']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256063, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "990b479bceee49f44499cea2294433f0" } } $('.js-work-strip[data-work-id=21256063]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256063,"title":"Expression of soluble recombinant TGF-尾 type II receptor fused with the Fc portion of human IgG1 (sT尾RII-Fc) in NS0 cells","translated_title":"","metadata":{"abstract":"We have constructed and expressed recombinant chimeric soluble TGF-尾 type II receptor fused with the Fc portion of human IgG1 (sT尾RII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. 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resistance to docetaxel-based chemotherapy in metastatic breast cancer patients correlates with a high frequency of BRCA1 mutations. Med Sci Monit 14(7):SC7-SC10" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256062/Primary_resistance_to_docetaxel_based_chemotherapy_in_metastatic_breast_cancer_patients_correlates_with_a_high_frequency_of_BRCA1_mutations_Med_Sci_Monit_14_7_SC7_SC10">Primary resistance to docetaxel-based chemotherapy in metastatic breast cancer patients correlates with a high frequency of BRCA1 mutations. Med Sci Monit 14(7):SC7-SC10</a></div><div class="wp-workCard_item"><span>Medical science monitor: international medical journal of experimental and clinical research</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Docetaxel is a member of the taxoid anticancer agents routinely used in the treatment of high-ris...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Docetaxel is a member of the taxoid anticancer agents routinely used in the treatment of high-risk or metastatic breast cancer. A recent study demonstrated that a low response rate to taxane-based neoadjuvant chemotherapy is associated with BRCA1 mutations. Therefore the frequency of BRCA1 mutations in a population of metastatic docetaxel-refractory patients was analyzed. Nineteen treatment-refractory patients were selected from a group of 175 metastatic breast cancer patients treated with docetaxel-based therapy. DNA isolated from blood or from paraffin-embedded tissue samples was screened for three founder mutations common (&gt;80%) in the Polish population. Additionally, the frequency of BRCA1 mutations was compared with the particular phenotype of breast cancer cells. BRCA1 mutations were found in 5 of the 19 of patients (26.3%), all 5 being of the 7 patients with triple-negative breast cancer (71%, p&lt;0.002). This study indicates that the administration of taxanes, especially...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256062"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256062"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256062; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256062]").text(description); $(".js-view-count[data-work-id=21256062]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256062; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256062']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256062, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256062]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256062,"title":"Primary resistance to docetaxel-based chemotherapy in metastatic breast cancer patients correlates with a high frequency of BRCA1 mutations. 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BRCA1 mutations were found in 5 of the 19 of patients (26.3%), all 5 being of the 7 patients with triple-negative breast cancer (71%, p\u0026lt;0.002). This study indicates that the administration of taxanes, especially...","publication_name":"Medical science monitor: international medical journal of experimental and clinical research"},"translated_abstract":"Docetaxel is a member of the taxoid anticancer agents routinely used in the treatment of high-risk or metastatic breast cancer. A recent study demonstrated that a low response rate to taxane-based neoadjuvant chemotherapy is associated with BRCA1 mutations. Therefore the frequency of BRCA1 mutations in a population of metastatic docetaxel-refractory patients was analyzed. Nineteen treatment-refractory patients were selected from a group of 175 metastatic breast cancer patients treated with docetaxel-based therapy. DNA isolated from blood or from paraffin-embedded tissue samples was screened for three founder mutations common (\u0026gt;80%) in the Polish population. Additionally, the frequency of BRCA1 mutations was compared with the particular phenotype of breast cancer cells. BRCA1 mutations were found in 5 of the 19 of patients (26.3%), all 5 being of the 7 patients with triple-negative breast cancer (71%, p\u0026lt;0.002). This study indicates that the administration of taxanes, especially...","internal_url":"https://www.academia.edu/21256062/Primary_resistance_to_docetaxel_based_chemotherapy_in_metastatic_breast_cancer_patients_correlates_with_a_high_frequency_of_BRCA1_mutations_Med_Sci_Monit_14_7_SC7_SC10","translated_internal_url":"","created_at":"2016-01-30T11:06:53.295-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260693,"work_id":21256062,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":1889654,"email":"p***i@ump.edu.pl","display_order":0,"name":"Piotr Wysocki","title":"Primary resistance to docetaxel-based chemotherapy in metastatic breast cancer patients correlates with a high frequency of BRCA1 mutations. Med Sci Monit 14(7):SC7-SC10"}],"downloadable_attachments":[],"slug":"Primary_resistance_to_docetaxel_based_chemotherapy_in_metastatic_breast_cancer_patients_correlates_with_a_high_frequency_of_BRCA1_mutations_Med_Sci_Monit_14_7_SC7_SC10","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":42380325,"first_name":"Andrzej","middle_initials":null,"last_name":"Mackiewicz","page_name":"AndrzejMackiewicz","domain_name":"independent","created_at":"2016-01-30T11:05:19.160-08:00","display_name":"Andrzej Mackiewicz","url":"https://independent.academia.edu/AndrzejMackiewicz"},"attachments":[],"research_interests":[{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged"},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256061"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21256061/The_importance_of_ceruloplasmin_oxidase_activity_in_patients_with_chronic_lower_limb_atherosclerotic_ischemia"><img alt="Research paper thumbnail of The importance of ceruloplasmin oxidase activity in patients with chronic lower limb atherosclerotic ischemia" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256061/The_importance_of_ceruloplasmin_oxidase_activity_in_patients_with_chronic_lower_limb_atherosclerotic_ischemia">The importance of ceruloplasmin oxidase activity in patients with chronic lower limb atherosclerotic ischemia</a></div><div class="wp-workCard_item"><span>International angiology: a journal of the International Union of Angiology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Inflammatory reactions accompanying ischemia increase the cytokine synthesis what leads to the in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Inflammatory reactions accompanying ischemia increase the cytokine synthesis what leads to the increase in serum ceruloplasmin (Cp) concentration and activity. The aim of the study was to evaluate the association between serum Cp oxidase concentration and activity and the grade of lower extremity ischemia. Moreover, the correlation of Cp concentration and activity with the levels of interleukin 6 (IL-6), C-reacting protein (CRP), and a-1-acid glycoprotein (AGP) in serum was studied. Two groups of patients were examined: 15 patients with moderate (MI) and 32 patients with critical ischemia (CI) of the lower extremities. Cp oxidase activity was measured spectrophotometrically, after incubation with o-dianisidine. The concentration of IL-6 was measured with the ELISA method, and Cp, CRP and AGP concentration by rocket immunoelectrophoresis. Significant increase in Cp oxidase concentration and activity was observed in patients with critical limb ischemia (median: 164.8 U/L), especially ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256061"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256061"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256061; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256061]").text(description); $(".js-view-count[data-work-id=21256061]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256061; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256061']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256061, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256061]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256061,"title":"The importance of ceruloplasmin oxidase activity in patients with chronic lower limb atherosclerotic ischemia","translated_title":"","metadata":{"abstract":"Inflammatory reactions accompanying ischemia increase the cytokine synthesis what leads to the increase in serum ceruloplasmin (Cp) concentration and activity. The aim of the study was to evaluate the association between serum Cp oxidase concentration and activity and the grade of lower extremity ischemia. Moreover, the correlation of Cp concentration and activity with the levels of interleukin 6 (IL-6), C-reacting protein (CRP), and a-1-acid glycoprotein (AGP) in serum was studied. Two groups of patients were examined: 15 patients with moderate (MI) and 32 patients with critical ischemia (CI) of the lower extremities. Cp oxidase activity was measured spectrophotometrically, after incubation with o-dianisidine. The concentration of IL-6 was measured with the ELISA method, and Cp, CRP and AGP concentration by rocket immunoelectrophoresis. Significant increase in Cp oxidase concentration and activity was observed in patients with critical limb ischemia (median: 164.8 U/L), especially ...","publication_name":"International angiology: a journal of the International Union of Angiology"},"translated_abstract":"Inflammatory reactions accompanying ischemia increase the cytokine synthesis what leads to the increase in serum ceruloplasmin (Cp) concentration and activity. The aim of the study was to evaluate the association between serum Cp oxidase concentration and activity and the grade of lower extremity ischemia. Moreover, the correlation of Cp concentration and activity with the levels of interleukin 6 (IL-6), C-reacting protein (CRP), and a-1-acid glycoprotein (AGP) in serum was studied. Two groups of patients were examined: 15 patients with moderate (MI) and 32 patients with critical ischemia (CI) of the lower extremities. Cp oxidase activity was measured spectrophotometrically, after incubation with o-dianisidine. The concentration of IL-6 was measured with the ELISA method, and Cp, CRP and AGP concentration by rocket immunoelectrophoresis. Significant increase in Cp oxidase concentration and activity was observed in patients with critical limb ischemia (median: 164.8 U/L), especially ...","internal_url":"https://www.academia.edu/21256061/The_importance_of_ceruloplasmin_oxidase_activity_in_patients_with_chronic_lower_limb_atherosclerotic_ischemia","translated_internal_url":"","created_at":"2016-01-30T11:06:53.157-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260735,"work_id":21256061,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":3311240,"email":"c***l@wp.pl","display_order":0,"name":"Ryszard Staniszewski","title":"The importance of ceruloplasmin oxidase activity in patients with chronic lower limb atherosclerotic ischemia"}],"downloadable_attachments":[],"slug":"The_importance_of_ceruloplasmin_oxidase_activity_in_patients_with_chronic_lower_limb_atherosclerotic_ischemia","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":42380325,"first_name":"Andrzej","middle_initials":null,"last_name":"Mackiewicz","page_name":"AndrzejMackiewicz","domain_name":"independent","created_at":"2016-01-30T11:05:19.160-08:00","display_name":"Andrzej Mackiewicz","url":"https://independent.academia.edu/AndrzejMackiewicz"},"attachments":[],"research_interests":[{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":82983,"name":"Ischemia","url":"https://www.academia.edu/Documents/in/Ischemia"},{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged"},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged"},{"id":295452,"name":"C reactive protein","url":"https://www.academia.edu/Documents/in/C_reactive_protein"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult"},{"id":383086,"name":"Ceruloplasmin","url":"https://www.academia.edu/Documents/in/Ceruloplasmin"},{"id":462777,"name":"Leg","url":"https://www.academia.edu/Documents/in/Leg"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256060"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21256060/Targeted_therapy_of_renal_cell_cancer"><img alt="Research paper thumbnail of Targeted therapy of renal cell cancer" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256060/Targeted_therapy_of_renal_cell_cancer">Targeted therapy of renal cell cancer</a></div><div class="wp-workCard_item"><span>Current opinion in investigational drugs (London, England: 2000)</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent year...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent years. Elucidation of the crucial role of the Von Hippel-Lindau (VHL) tumor suppressor gene in upregulating growth factors associated with angiogenesis has provided new insight into RCC biology and has identified specific targets for novel therapeutic strategies. For almost two decades, cytokine-based immunotherapy has remained a treatment of choice in advanced RCC patients. However, it has provided only modest improvement in clinical outcome and has been associated with severe toxicity. With the advent of novel therapies directly targeting the VEGF molecule or VEGF receptor signal transduction pathway, the clinical outcome in high-risk, advanced RCC has significantly improved. In phase III clinical trials, novel targeted agents - temsirolimus, sorafenib, sunitinib and bevacizumab - significantly prolonged progression-free survival of patients with metastatic RCC and, crucially, temsirolimus...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256060"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256060"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256060; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256060]").text(description); $(".js-view-count[data-work-id=21256060]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256060; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256060']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256060, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256060]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256060,"title":"Targeted therapy of renal cell cancer","translated_title":"","metadata":{"abstract":"Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent years. Elucidation of the crucial role of the Von Hippel-Lindau (VHL) tumor suppressor gene in upregulating growth factors associated with angiogenesis has provided new insight into RCC biology and has identified specific targets for novel therapeutic strategies. For almost two decades, cytokine-based immunotherapy has remained a treatment of choice in advanced RCC patients. However, it has provided only modest improvement in clinical outcome and has been associated with severe toxicity. With the advent of novel therapies directly targeting the VEGF molecule or VEGF receptor signal transduction pathway, the clinical outcome in high-risk, advanced RCC has significantly improved. In phase III clinical trials, novel targeted agents - temsirolimus, sorafenib, sunitinib and bevacizumab - significantly prolonged progression-free survival of patients with metastatic RCC and, crucially, temsirolimus...","publication_name":"Current opinion in investigational drugs (London, England: 2000)"},"translated_abstract":"Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent years. Elucidation of the crucial role of the Von Hippel-Lindau (VHL) tumor suppressor gene in upregulating growth factors associated with angiogenesis has provided new insight into RCC biology and has identified specific targets for novel therapeutic strategies. For almost two decades, cytokine-based immunotherapy has remained a treatment of choice in advanced RCC patients. However, it has provided only modest improvement in clinical outcome and has been associated with severe toxicity. With the advent of novel therapies directly targeting the VEGF molecule or VEGF receptor signal transduction pathway, the clinical outcome in high-risk, advanced RCC has significantly improved. In phase III clinical trials, novel targeted agents - temsirolimus, sorafenib, sunitinib and bevacizumab - significantly prolonged progression-free survival of patients with metastatic RCC and, crucially, temsirolimus...","internal_url":"https://www.academia.edu/21256060/Targeted_therapy_of_renal_cell_cancer","translated_internal_url":"","created_at":"2016-01-30T11:06:52.977-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260692,"work_id":21256060,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":1889654,"email":"p***i@ump.edu.pl","display_order":0,"name":"Piotr Wysocki","title":"Targeted therapy of renal cell cancer"}],"downloadable_attachments":[],"slug":"Targeted_therapy_of_renal_cell_cancer","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":42380325,"first_name":"Andrzej","middle_initials":null,"last_name":"Mackiewicz","page_name":"AndrzejMackiewicz","domain_name":"independent","created_at":"2016-01-30T11:05:19.160-08:00","display_name":"Andrzej Mackiewicz","url":"https://independent.academia.edu/AndrzejMackiewicz"},"attachments":[],"research_interests":[{"id":9111,"name":"Cytokines","url":"https://www.academia.edu/Documents/in/Cytokines"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":105308,"name":"Renal cell Carcinoma","url":"https://www.academia.edu/Documents/in/Renal_cell_Carcinoma"},{"id":159187,"name":"Drug Delivery 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Part II]</a></div><div class="wp-workCard_item"><span>Post臋py Higieny i Medycyny Do艣wiadczalnej (Advances in Hygiene and Experimental Medicine)</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The development and progress in engineered spider silk manufacturing has enabled its practical ap...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The development and progress in engineered spider silk manufacturing has enabled its practical application. Recombinant spider silk can assemble in several morphological forms such as films, hydrogels, fibers, scaffolds, microcapsules, and micro- and nanospheres. The in vitro fiber formation takes place by mimicking the natural spinning process in the spider spinning gland: in the presence of phosphate ions and dragging forces. Films are obtained by evaporation of solvent from the silk solution, while the result of evaporation of the solvent in the presence of porogens is a silk scaffold. Hydrogels are formed by spontaneous polymerization of silk particles in solutions at low pH. The silk film assembled at the interface of two immiscible phases forms microcapsules. The smallest of the described forms--silk spheres--are obtained by salting out the silk protein solution after addition of the phosphate ions. 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The smallest of the described forms--silk spheres--are obtained by salting out the silk protein solution after addition of the phosphate ions. 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Part I]" class="work-thumbnail" src="https://attachments.academia-assets.com/41784738/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256056/_Engineered_spider_silk_the_intelligent_biomaterial_of_the_future_Part_I_">[Engineered spider silk: the intelligent biomaterial of the future. Part I]</a></div><div class="wp-workCard_item"><span>Post臋py Higieny i Medycyny Do艣wiadczalnej (Advances in Hygiene and Experimental Medicine)</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The unique properties of spider silk such as strength, extensibility, toughness, biocompatibility...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The unique properties of spider silk such as strength, extensibility, toughness, biocompatibility and biodegradability are the reasons for the recent development in silk biomaterial technology. For a long time scientific progress was impeded by limited access to spider silk. However, the development of the molecular biology strategy was a breaking point in synthetic spider silk protein design. The sequences of engineered spider silk are based on the consensus motives of the corresponding natural equivalents. Moreover, the engineered silk proteins may be modified in order to gain a new function. The strategy of the hybrid proteins constructed on the DNA level combines the sequence of engineered silk, which is responsible for the biomaterial structure, with the sequence of polypeptide which allows functionalization of the silk biomaterial. The functional domains may comprise receptor binding sites, enzymes, metal or sugar binding sites and others. Currently, advanced research is being...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="60b221419315a0813ab14aabe05c6e76" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784738,"asset_id":21256056,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784738/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256056"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256056"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256056; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256056]").text(description); $(".js-view-count[data-work-id=21256056]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256056; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256056']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256056, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "60b221419315a0813ab14aabe05c6e76" } } $('.js-work-strip[data-work-id=21256056]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256056,"title":"[Engineered spider silk: the intelligent biomaterial of the future. 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The functional domains may comprise receptor binding sites, enzymes, metal or sugar binding sites and others. Currently, advanced research is being...","publication_name":"Post臋py Higieny i Medycyny Do艣wiadczalnej (Advances in Hygiene and Experimental Medicine)"},"translated_abstract":"The unique properties of spider silk such as strength, extensibility, toughness, biocompatibility and biodegradability are the reasons for the recent development in silk biomaterial technology. For a long time scientific progress was impeded by limited access to spider silk. However, the development of the molecular biology strategy was a breaking point in synthetic spider silk protein design. The sequences of engineered spider silk are based on the consensus motives of the corresponding natural equivalents. Moreover, the engineered silk proteins may be modified in order to gain a new function. The strategy of the hybrid proteins constructed on the DNA level combines the sequence of engineered silk, which is responsible for the biomaterial structure, with the sequence of polypeptide which allows functionalization of the silk biomaterial. The functional domains may comprise receptor binding sites, enzymes, metal or sugar binding sites and others. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256055"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21256055/Optimization_of_a_retroviral_vector_for_transduction_of_human_CD34_positive_cells"><img alt="Research paper thumbnail of Optimization of a retroviral vector for transduction of human CD34 positive cells" class="work-thumbnail" src="https://attachments.academia-assets.com/41784732/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256055/Optimization_of_a_retroviral_vector_for_transduction_of_human_CD34_positive_cells">Optimization of a retroviral vector for transduction of human CD34 positive cells</a></div><div class="wp-workCard_item"><span>Acta biochimica Polonica</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human stem and progenitor cells have recently become objects of intensive studies as an important...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy and regenerative medicine. Retroviral vectors are among the most effective tools for genetic modification of these cells. However, their transduction efficiency strongly depends on the choice of the ex vivo transduction system. The aim of this study was to elaborate a system for retroviral vector transduction of human CD34 positive cells isolated from cord blood. The retroviral vector pMINV EGFP was chosen for transduction of two human erythroblastoid cell lines: KG-1a (CD34 positive) and K562 (CD34 negative). For vector construction, three promoters and two retroviral vector packaging cell lines were used. To optimize the physicochemical conditions of the transduction process, different temperatures of supernatant harvesting, the influence of centrifugation and the presence of transduction enhancing agents were tested. The conditions elaborated with KG-1a cells ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9ebe7539aef04186cfcfee31d9089083" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784732,"asset_id":21256055,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784732/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256055"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256055"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256055; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256055]").text(description); $(".js-view-count[data-work-id=21256055]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256055; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256055']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256055, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9ebe7539aef04186cfcfee31d9089083" } } $('.js-work-strip[data-work-id=21256055]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256055,"title":"Optimization of a retroviral vector for transduction of human CD34 positive cells","translated_title":"","metadata":{"abstract":"Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy and regenerative medicine. Retroviral vectors are among the most effective tools for genetic modification of these cells. However, their transduction efficiency strongly depends on the choice of the ex vivo transduction system. The aim of this study was to elaborate a system for retroviral vector transduction of human CD34 positive cells isolated from cord blood. The retroviral vector pMINV EGFP was chosen for transduction of two human erythroblastoid cell lines: KG-1a (CD34 positive) and K562 (CD34 negative). For vector construction, three promoters and two retroviral vector packaging cell lines were used. To optimize the physicochemical conditions of the transduction process, different temperatures of supernatant harvesting, the influence of centrifugation and the presence of transduction enhancing agents were tested. The conditions elaborated with KG-1a cells ...","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Acta biochimica Polonica"},"translated_abstract":"Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy and regenerative medicine. Retroviral vectors are among the most effective tools for genetic modification of these cells. However, their transduction efficiency strongly depends on the choice of the ex vivo transduction system. The aim of this study was to elaborate a system for retroviral vector transduction of human CD34 positive cells isolated from cord blood. The retroviral vector pMINV EGFP was chosen for transduction of two human erythroblastoid cell lines: KG-1a (CD34 positive) and K562 (CD34 negative). For vector construction, three promoters and two retroviral vector packaging cell lines were used. To optimize the physicochemical conditions of the transduction process, different temperatures of supernatant harvesting, the influence of centrifugation and the presence of transduction enhancing agents were tested. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256054"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21256054/Expression_of_soluble_recombinant_TGF_beta_type_II_receptor_fused_with_the_Fc_portion_of_human_IgG1_sTbetaRII_Fc_in_NS0_cells"><img alt="Research paper thumbnail of Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells" class="work-thumbnail" src="https://attachments.academia-assets.com/41784737/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256054/Expression_of_soluble_recombinant_TGF_beta_type_II_receptor_fused_with_the_Fc_portion_of_human_IgG1_sTbetaRII_Fc_in_NS0_cells">Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells</a></div><div class="wp-workCard_item"><span>Acta biochimica Polonica</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused wi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTbetaRII-Fc binds native TGF-beta1 and TGF-beta3 isoforms and neutralizes their activity in vitro.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="84de84c10785f6abe3b27b4adacc98a8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784737,"asset_id":21256054,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784737/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256054"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256054"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256054; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256054]").text(description); $(".js-view-count[data-work-id=21256054]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256054; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256054']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256054, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "84de84c10785f6abe3b27b4adacc98a8" } } $('.js-work-strip[data-work-id=21256054]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256054,"title":"Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells","translated_title":"","metadata":{"abstract":"We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTbetaRII-Fc binds native TGF-beta1 and TGF-beta3 isoforms and neutralizes their activity in vitro.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Acta biochimica Polonica"},"translated_abstract":"We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTbetaRII-Fc binds native TGF-beta1 and TGF-beta3 isoforms and neutralizes their activity in vitro.","internal_url":"https://www.academia.edu/21256054/Expression_of_soluble_recombinant_TGF_beta_type_II_receptor_fused_with_the_Fc_portion_of_human_IgG1_sTbetaRII_Fc_in_NS0_cells","translated_internal_url":"","created_at":"2016-01-30T11:06:51.951-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260715,"work_id":21256054,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":3311237,"email":"k***r@gmail.com","display_order":0,"name":"Dariusz Kowalczyk","title":"Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 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Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256053"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21256053/BRCA2_mutations_and_androgen_receptor_expression_as_independent_predictors_of_outcome_of_male_breast_cancer_patients"><img alt="Research paper thumbnail of BRCA2 mutations and androgen receptor expression as independent predictors of outcome of male breast cancer patients" class="work-thumbnail" src="https://attachments.academia-assets.com/41784736/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256053/BRCA2_mutations_and_androgen_receptor_expression_as_independent_predictors_of_outcome_of_male_breast_cancer_patients">BRCA2 mutations and androgen receptor expression as independent predictors of outcome of male breast cancer patients</a></div><div class="wp-workCard_item"><span>Clinical cancer research : an official journal of the American Association for Cancer Research</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Germline mutations of the BRCA2 gene are involved in the development of a considerable number of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Germline mutations of the BRCA2 gene are involved in the development of a considerable number of male breast cancer cases. Although phenotypic differences have been observed between sporadic and BRCA-related breast carcinomas, conflicting data exist on the differences in prognosis of women with hereditary and sporadic breast cancer. The purpose of the study was to investigate the prognostic value of BRCA2 status in male breast carcinoma (MBC). We studied 43 male breast cancer patients, including 12 with BRCA2 mutations. Tumor samples were characterized immunohistochemically using antibodies to estrogen receptor, progesterone receptor, and androgen receptor (AR). BRCA2-related tumors presented at the earlier age compared with sporadic tumors (P = 0.005). Patients positive and negative for BRCA2 mutations did not differ with respect to tumor size, lymph node involvement, histological grade, and sex hormone receptor status. Five-year disease-free survival (DFS) and overall survival (OS...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2a59d5310ee1c2bdcd9d0ba8dffa53bf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784736,"asset_id":21256053,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784736/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256053"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256053"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256053; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256053]").text(description); $(".js-view-count[data-work-id=21256053]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256053; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256053']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256053, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2a59d5310ee1c2bdcd9d0ba8dffa53bf" } } $('.js-work-strip[data-work-id=21256053]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256053,"title":"BRCA2 mutations and androgen receptor expression as independent predictors of outcome of male breast cancer patients","translated_title":"","metadata":{"abstract":"Germline mutations of the BRCA2 gene are involved in the development of a considerable number of male breast cancer cases. Although phenotypic differences have been observed between sporadic and BRCA-related breast carcinomas, conflicting data exist on the differences in prognosis of women with hereditary and sporadic breast cancer. The purpose of the study was to investigate the prognostic value of BRCA2 status in male breast carcinoma (MBC). We studied 43 male breast cancer patients, including 12 with BRCA2 mutations. Tumor samples were characterized immunohistochemically using antibodies to estrogen receptor, progesterone receptor, and androgen receptor (AR). BRCA2-related tumors presented at the earlier age compared with sporadic tumors (P = 0.005). Patients positive and negative for BRCA2 mutations did not differ with respect to tumor size, lymph node involvement, histological grade, and sex hormone receptor status. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="4511300" id="papers"><div class="js-work-strip profile--work_container" data-work-id="38621338"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/38621338/Novel_BRCA1_mutations_and_more_frequent_intron_20_alteration_found_among_236_women_from_Western_Poland"><img alt="Research paper thumbnail of Novel BRCA1 mutations and more frequent intron-20 alteration found among 236 women from Western Poland" class="work-thumbnail" src="https://attachments.academia-assets.com/58698748/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/38621338/Novel_BRCA1_mutations_and_more_frequent_intron_20_alteration_found_among_236_women_from_Western_Poland">Novel BRCA1 mutations and more frequent intron-20 alteration found among 236 women from Western Poland</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AndrzejMackiewicz">Andrzej Mackiewicz</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://torun-pl.academia.edu/MarcinWo%C5%BAniak">Marcin Wo藕niak</a></span></div><div class="wp-workCard_item"><span>Oncogene</span><span>, 1997</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d1d288a2f499984f7f9c180b12373e57" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":58698748,"asset_id":38621338,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/58698748/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="38621338"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="38621338"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 38621338; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="32623469"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/32623469/EPR_Study_of_Iron_Ion_Complexes_in_Human_Blood"><img alt="Research paper thumbnail of EPR Study of Iron Ion Complexes in Human Blood" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/32623469/EPR_Study_of_Iron_Ion_Complexes_in_Human_Blood">EPR Study of Iron Ion Complexes in Human Blood</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AndrzejMackiewicz">Andrzej Mackiewicz</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/EwaLeporowska">Ewa Leporowska</a></span></div><div class="wp-workCard_item"><span>Applied Magnetic Resonance</span><span>, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Electronic states of iron ion complexes in human blood from patients with melanoma have been inve...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Electronic states of iron ion complexes in human blood from patients with melanoma have been investigated by electron paramagnetic resonance (EPR). The measurements were performed at liquid nitrogen temperature (77聽K) on an X-band EPR spectrometer. Numerous types of iron paramagnetic centers have been identified. In several kinds of protein complexes exemplified by methemoglobin, transferrin or ferritin, various forms of trivalent iron have been found. Three groups of patients with typical EPR spectra have been individualized. These groups differed in types and concentration of paramagnetic centers in peripheral blood. 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High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo. Downregulation of TRIM28 expression in xenografts led to deceased expression of pluripotency and mesenchymal markers, as well as inhibition of signaling pathways involved in the complex mechanism of CSC maintenance. Moreover, TRIM28 depletion reduced the ability of cancer cells to induce tumor growth when subcutaneously injected in limiting dilutions. Our data demonstrate that the downregulation of TRIM28 gene expression reduced the ability of CSCs to self-renew that resulted in significant reduction of tumor growth. Loss of function of TRIM28 leads to dysregulation of cell cycle, cellular response to stress, cancer cell metabolism, and inhibition of oxidative phosphorylation. All these mechanisms directly regulate maintenance of CSC population. Our original results revealed the role of the TRIM28 in regulating the CSC population in breast cancer. These findings may pave the way to novel and more effective therapies targeting cancer stem cells in breast tumors.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="31510456"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="31510456"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 31510456; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=31510456]").text(description); $(".js-view-count[data-work-id=31510456]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 31510456; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='31510456']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 31510456, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=31510456]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":31510456,"title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development","translated_title":"","metadata":{"abstract":"The expression of Tripartite motif-containing protein 28 (TRIM28)/Kr眉ppel-associated box (KRAB)-associated protein 1 (KAP1), is elevated in at least 14 tumor types, including solid and hematopoietic tumors. High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo. Downregulation of TRIM28 expression in xenografts led to deceased expression of pluripotency and mesenchymal markers, as well as inhibition of signaling pathways involved in the complex mechanism of CSC maintenance. Moreover, TRIM28 depletion reduced the ability of cancer cells to induce tumor growth when subcutaneously injected in limiting dilutions. Our data demonstrate that the downregulation of TRIM28 gene expression reduced the ability of CSCs to self-renew that resulted in significant reduction of tumor growth. Loss of function of TRIM28 leads to dysregulation of cell cycle, cellular response to stress, cancer cell metabolism, and inhibition of oxidative phosphorylation. All these mechanisms directly regulate maintenance of CSC population. Our original results revealed the role of the TRIM28 in regulating the CSC population in breast cancer. These findings may pave the way to novel and more effective therapies targeting cancer stem cells in breast tumors.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Oncotarget"},"translated_abstract":"The expression of Tripartite motif-containing protein 28 (TRIM28)/Kr眉ppel-associated box (KRAB)-associated protein 1 (KAP1), is elevated in at least 14 tumor types, including solid and hematopoietic tumors. High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo. Downregulation of TRIM28 expression in xenografts led to deceased expression of pluripotency and mesenchymal markers, as well as inhibition of signaling pathways involved in the complex mechanism of CSC maintenance. Moreover, TRIM28 depletion reduced the ability of cancer cells to induce tumor growth when subcutaneously injected in limiting dilutions. Our data demonstrate that the downregulation of TRIM28 gene expression reduced the ability of CSCs to self-renew that resulted in significant reduction of tumor growth. Loss of function of TRIM28 leads to dysregulation of cell cycle, cellular response to stress, cancer cell metabolism, and inhibition of oxidative phosphorylation. All these mechanisms directly regulate maintenance of CSC population. Our original results revealed the role of the TRIM28 in regulating the CSC population in breast cancer. These findings may pave the way to novel and more effective therapies targeting cancer stem cells in breast tumors.","internal_url":"https://www.academia.edu/31510456/TRIM28_multi_domain_protein_regulates_cancer_stem_cell_population_in_breast_tumor_development","translated_internal_url":"","created_at":"2017-02-18T20:04:36.815-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":60298097,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":27691240,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":null,"co_author_invite_id":6060537,"email":"p***2@mdanderson.org","display_order":0,"name":"Parantu Shah","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691246,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":null,"co_author_invite_id":6060539,"email":"s***k@gmail.com","display_order":4194304,"name":"Sylwia Mazurek","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691248,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":null,"co_author_invite_id":6060541,"email":"m***i@interia.pl","display_order":6291456,"name":"Marta Klimczak","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691254,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":null,"co_author_invite_id":6060545,"email":"s***r@gmail.com","display_order":7340032,"name":"Patrycja Czerwi艅ska","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691324,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":null,"co_author_invite_id":2320710,"email":"v***s@wp.pl","display_order":7864320,"name":"Violetta Filas","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691337,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":null,"co_author_invite_id":3836047,"email":"s***a@gmail.com","display_order":8126464,"name":"Barbara Soza艅ska","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691348,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":57163307,"co_author_invite_id":null,"email":"t***2@gmail.com","display_order":8257536,"name":"Katarzyna Tomczak","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691349,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":3090642,"co_author_invite_id":null,"email":"p***k@gmail.com","display_order":8323072,"name":"Przemyslaw Biecek","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"},{"id":27691353,"work_id":31510456,"tagging_user_id":60298097,"tagged_user_id":42380325,"co_author_invite_id":null,"email":"a***c@amu.edu.pl","display_order":8355840,"name":"Andrzej Mackiewicz","title":"TRIM28 multi-domain protein regulates cancer stem cell population in breast tumor development"}],"downloadable_attachments":[],"slug":"TRIM28_multi_domain_protein_regulates_cancer_stem_cell_population_in_breast_tumor_development","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":60298097,"first_name":"Jannik","middle_initials":null,"last_name":"Andersen","page_name":"JannikNAndersen","domain_name":"independent","created_at":"2017-02-18T20:03:07.560-08:00","display_name":"Jannik Andersen","url":"https://independent.academia.edu/JannikNAndersen"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256067"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21256067/Gene_modified_tumor_vaccines_in_therapy_of_malignant_melanoma"><img alt="Research paper thumbnail of Gene modified tumor vaccines in therapy of malignant melanoma" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256067/Gene_modified_tumor_vaccines_in_therapy_of_malignant_melanoma">Gene modified tumor vaccines in therapy of malignant melanoma</a></div><div class="wp-workCard_item"><span>Otolaryngologia polska. The Polish otolaryngology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Over the years the incidence of malignant melanoma in Poland as well as in other countries has be...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Over the years the incidence of malignant melanoma in Poland as well as in other countries has been continuously increasing. Surgery is a treatment of choice in the early stages of primary lesions. Advanced malignant melanoma however is resistant to chemotherapy or radiotherapy. Therefore there is a need for new, more effective treatments. In the last years biotherapy such as immunotherapy is focusing a lot of attention. Unfortunately, systemic administration of immunostimulatory factors is very often associated with severe side effects. Thus, concepts of specific immunotherapies such as immunogene therapy have been developed. Currently, various gene therapy strategies of malignant melanoma are being evaluated in multiple clinical trials carried out all over the world. They include gene modified cancer vaccines (GMTV) modified with genes encoding (i) cytokines or (ii) costimulatory molecules and dendritic cells modified with (iii) genes encoding tumor antigens or (iv) immunostimulatory factors. Since January 1996 in Department of Cancer Immunology USOMS, at GreatPoland Cancer Center in Poznan, Poland a GMTV has been tested in malignant melanoma patients. For the last 6 years more than 220 patients were enrolled into study of GMTV consisting of melanoma cells modified with genes encoding IL-6 and its agonistic soluble receptor (sIL-6R). More than 25% of objective clinical responses and significant life extension were observed. The encouraging results formed a basis for design of a phase III prospective, randomized clinical study.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256067"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256067"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256067; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256067]").text(description); $(".js-view-count[data-work-id=21256067]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256067; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256067']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256067, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256067]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256067,"title":"Gene modified tumor vaccines in therapy of malignant melanoma","translated_title":"","metadata":{"abstract":"Over the years the incidence of malignant melanoma in Poland as well as in other countries has been continuously increasing. Surgery is a treatment of choice in the early stages of primary lesions. Advanced malignant melanoma however is resistant to chemotherapy or radiotherapy. Therefore there is a need for new, more effective treatments. In the last years biotherapy such as immunotherapy is focusing a lot of attention. Unfortunately, systemic administration of immunostimulatory factors is very often associated with severe side effects. Thus, concepts of specific immunotherapies such as immunogene therapy have been developed. Currently, various gene therapy strategies of malignant melanoma are being evaluated in multiple clinical trials carried out all over the world. They include gene modified cancer vaccines (GMTV) modified with genes encoding (i) cytokines or (ii) costimulatory molecules and dendritic cells modified with (iii) genes encoding tumor antigens or (iv) immunostimulatory factors. Since January 1996 in Department of Cancer Immunology USOMS, at GreatPoland Cancer Center in Poznan, Poland a GMTV has been tested in malignant melanoma patients. For the last 6 years more than 220 patients were enrolled into study of GMTV consisting of melanoma cells modified with genes encoding IL-6 and its agonistic soluble receptor (sIL-6R). More than 25% of objective clinical responses and significant life extension were observed. The encouraging results formed a basis for design of a phase III prospective, randomized clinical study.","publication_name":"Otolaryngologia polska. The Polish otolaryngology"},"translated_abstract":"Over the years the incidence of malignant melanoma in Poland as well as in other countries has been continuously increasing. Surgery is a treatment of choice in the early stages of primary lesions. Advanced malignant melanoma however is resistant to chemotherapy or radiotherapy. Therefore there is a need for new, more effective treatments. 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Ocena efektu przeciwnowotworowego genetycznie modyfikowanej szczepionki kom贸rkowej w mysim modelu raka nerki" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256066/239_Ocena_efektu_przeciwnowotworowego_genetycznie_modyfikowanej_szczepionki_kom%C3%B3rkowej_w_mysim_modelu_raka_nerki">239. Ocena efektu przeciwnowotworowego genetycznie modyfikowanej szczepionki kom贸rkowej w mysim modelu raka nerki</a></div><div class="wp-workCard_item"><span>Reports of Practical Oncology & Radiotherapy</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256066]").text(description); $(".js-view-count[data-work-id=21256066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256066, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256066,"title":"239. Ocena efektu przeciwnowotworowego genetycznie modyfikowanej szczepionki kom贸rkowej w mysim modelu raka nerki","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"Reports of Practical Oncology \u0026 Radiotherapy"},"translated_abstract":null,"internal_url":"https://www.academia.edu/21256066/239_Ocena_efektu_przeciwnowotworowego_genetycznie_modyfikowanej_szczepionki_kom%C3%B3rkowej_w_mysim_modelu_raka_nerki","translated_internal_url":"","created_at":"2016-01-30T11:06:54.139-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260662,"work_id":21256066,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":2938834,"email":"p***k@beck.pl","display_order":0,"name":"Piotr Grabarczyk","title":"239. 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Adv Exp Med Biol. 2001;495:389-92. Improving the retroviral vector (RV) systems for immunother...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">1. Adv Exp Med Biol. 2001;495:389-92. Improving the retroviral vector (RV) systems for immunotherapy of cancer. Grabarczyk P, Gryska K, Wysocki PJ, Izycki D, Mackiewicz A. 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resistance to docetaxel-based chemotherapy in metastatic breast cancer patients correlates with a high frequency of BRCA1 mutations. Med Sci Monit 14(7):SC7-SC10" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256062/Primary_resistance_to_docetaxel_based_chemotherapy_in_metastatic_breast_cancer_patients_correlates_with_a_high_frequency_of_BRCA1_mutations_Med_Sci_Monit_14_7_SC7_SC10">Primary resistance to docetaxel-based chemotherapy in metastatic breast cancer patients correlates with a high frequency of BRCA1 mutations. Med Sci Monit 14(7):SC7-SC10</a></div><div class="wp-workCard_item"><span>Medical science monitor: international medical journal of experimental and clinical research</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Docetaxel is a member of the taxoid anticancer agents routinely used in the treatment of high-ris...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Docetaxel is a member of the taxoid anticancer agents routinely used in the treatment of high-risk or metastatic breast cancer. A recent study demonstrated that a low response rate to taxane-based neoadjuvant chemotherapy is associated with BRCA1 mutations. Therefore the frequency of BRCA1 mutations in a population of metastatic docetaxel-refractory patients was analyzed. Nineteen treatment-refractory patients were selected from a group of 175 metastatic breast cancer patients treated with docetaxel-based therapy. DNA isolated from blood or from paraffin-embedded tissue samples was screened for three founder mutations common (&gt;80%) in the Polish population. Additionally, the frequency of BRCA1 mutations was compared with the particular phenotype of breast cancer cells. BRCA1 mutations were found in 5 of the 19 of patients (26.3%), all 5 being of the 7 patients with triple-negative breast cancer (71%, p&lt;0.002). 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Significant increase in Cp oxidase concentration and activity was observed in patients with critical limb ischemia (median: 164.8 U/L), especially ...","publication_name":"International angiology: a journal of the International Union of Angiology"},"translated_abstract":"Inflammatory reactions accompanying ischemia increase the cytokine synthesis what leads to the increase in serum ceruloplasmin (Cp) concentration and activity. The aim of the study was to evaluate the association between serum Cp oxidase concentration and activity and the grade of lower extremity ischemia. Moreover, the correlation of Cp concentration and activity with the levels of interleukin 6 (IL-6), C-reacting protein (CRP), and a-1-acid glycoprotein (AGP) in serum was studied. Two groups of patients were examined: 15 patients with moderate (MI) and 32 patients with critical ischemia (CI) of the lower extremities. Cp oxidase activity was measured spectrophotometrically, after incubation with o-dianisidine. 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Significant increase in Cp oxidase concentration and activity was observed in patients with critical limb ischemia (median: 164.8 U/L), especially ...","internal_url":"https://www.academia.edu/21256061/The_importance_of_ceruloplasmin_oxidase_activity_in_patients_with_chronic_lower_limb_atherosclerotic_ischemia","translated_internal_url":"","created_at":"2016-01-30T11:06:53.157-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260735,"work_id":21256061,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":3311240,"email":"c***l@wp.pl","display_order":0,"name":"Ryszard Staniszewski","title":"The importance of ceruloplasmin oxidase activity in patients with chronic lower limb atherosclerotic ischemia"}],"downloadable_attachments":[],"slug":"The_importance_of_ceruloplasmin_oxidase_activity_in_patients_with_chronic_lower_limb_atherosclerotic_ischemia","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":42380325,"first_name":"Andrzej","middle_initials":null,"last_name":"Mackiewicz","page_name":"AndrzejMackiewicz","domain_name":"independent","created_at":"2016-01-30T11:05:19.160-08:00","display_name":"Andrzej Mackiewicz","url":"https://independent.academia.edu/AndrzejMackiewicz"},"attachments":[],"research_interests":[{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":82983,"name":"Ischemia","url":"https://www.academia.edu/Documents/in/Ischemia"},{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged"},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged"},{"id":295452,"name":"C reactive protein","url":"https://www.academia.edu/Documents/in/C_reactive_protein"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult"},{"id":383086,"name":"Ceruloplasmin","url":"https://www.academia.edu/Documents/in/Ceruloplasmin"},{"id":462777,"name":"Leg","url":"https://www.academia.edu/Documents/in/Leg"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256060"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21256060/Targeted_therapy_of_renal_cell_cancer"><img alt="Research paper thumbnail of Targeted therapy of renal cell cancer" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21256060/Targeted_therapy_of_renal_cell_cancer">Targeted therapy of renal cell cancer</a></div><div class="wp-workCard_item"><span>Current opinion in investigational drugs (London, England: 2000)</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent year...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent years. Elucidation of the crucial role of the Von Hippel-Lindau (VHL) tumor suppressor gene in upregulating growth factors associated with angiogenesis has provided new insight into RCC biology and has identified specific targets for novel therapeutic strategies. For almost two decades, cytokine-based immunotherapy has remained a treatment of choice in advanced RCC patients. However, it has provided only modest improvement in clinical outcome and has been associated with severe toxicity. With the advent of novel therapies directly targeting the VEGF molecule or VEGF receptor signal transduction pathway, the clinical outcome in high-risk, advanced RCC has significantly improved. In phase III clinical trials, novel targeted agents - temsirolimus, sorafenib, sunitinib and bevacizumab - significantly prolonged progression-free survival of patients with metastatic RCC and, crucially, temsirolimus...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256060"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256060"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256060; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256060]").text(description); $(".js-view-count[data-work-id=21256060]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256060; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256060']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256060, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21256060]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256060,"title":"Targeted therapy of renal cell cancer","translated_title":"","metadata":{"abstract":"Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent years. Elucidation of the crucial role of the Von Hippel-Lindau (VHL) tumor suppressor gene in upregulating growth factors associated with angiogenesis has provided new insight into RCC biology and has identified specific targets for novel therapeutic strategies. For almost two decades, cytokine-based immunotherapy has remained a treatment of choice in advanced RCC patients. However, it has provided only modest improvement in clinical outcome and has been associated with severe toxicity. With the advent of novel therapies directly targeting the VEGF molecule or VEGF receptor signal transduction pathway, the clinical outcome in high-risk, advanced RCC has significantly improved. In phase III clinical trials, novel targeted agents - temsirolimus, sorafenib, sunitinib and bevacizumab - significantly prolonged progression-free survival of patients with metastatic RCC and, crucially, temsirolimus...","publication_name":"Current opinion in investigational drugs (London, England: 2000)"},"translated_abstract":"Rapid development of treatment strategies for renal cell cancer (RCC) has occurred in recent years. Elucidation of the crucial role of the Von Hippel-Lindau (VHL) tumor suppressor gene in upregulating growth factors associated with angiogenesis has provided new insight into RCC biology and has identified specific targets for novel therapeutic strategies. For almost two decades, cytokine-based immunotherapy has remained a treatment of choice in advanced RCC patients. However, it has provided only modest improvement in clinical outcome and has been associated with severe toxicity. With the advent of novel therapies directly targeting the VEGF molecule or VEGF receptor signal transduction pathway, the clinical outcome in high-risk, advanced RCC has significantly improved. In phase III clinical trials, novel targeted agents - temsirolimus, sorafenib, sunitinib and bevacizumab - significantly prolonged progression-free survival of patients with metastatic RCC and, crucially, temsirolimus...","internal_url":"https://www.academia.edu/21256060/Targeted_therapy_of_renal_cell_cancer","translated_internal_url":"","created_at":"2016-01-30T11:06:52.977-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":42380325,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":14260692,"work_id":21256060,"tagging_user_id":42380325,"tagged_user_id":null,"co_author_invite_id":1889654,"email":"p***i@ump.edu.pl","display_order":0,"name":"Piotr Wysocki","title":"Targeted therapy of renal cell 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"profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256057"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21256057/_Engineered_spider_silk_the_intelligent_biomaterial_of_the_future_Part_II_"><img alt="Research paper thumbnail of [Engineered spider silk: the intelligent biomaterial of the future. 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Part II]</a></div><div class="wp-workCard_item"><span>Post臋py Higieny i Medycyny Do艣wiadczalnej (Advances in Hygiene and Experimental Medicine)</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The development and progress in engineered spider silk manufacturing has enabled its practical ap...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The development and progress in engineered spider silk manufacturing has enabled its practical application. Recombinant spider silk can assemble in several morphological forms such as films, hydrogels, fibers, scaffolds, microcapsules, and micro- and nanospheres. The in vitro fiber formation takes place by mimicking the natural spinning process in the spider spinning gland: in the presence of phosphate ions and dragging forces. Films are obtained by evaporation of solvent from the silk solution, while the result of evaporation of the solvent in the presence of porogens is a silk scaffold. Hydrogels are formed by spontaneous polymerization of silk particles in solutions at low pH. The silk film assembled at the interface of two immiscible phases forms microcapsules. The smallest of the described forms--silk spheres--are obtained by salting out the silk protein solution after addition of the phosphate ions. 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However, the development of the molecular biology strategy was a breaking point in synthetic spider silk protein design. The sequences of engineered spider silk are based on the consensus motives of the corresponding natural equivalents. Moreover, the engineered silk proteins may be modified in order to gain a new function. The strategy of the hybrid proteins constructed on the DNA level combines the sequence of engineered silk, which is responsible for the biomaterial structure, with the sequence of polypeptide which allows functionalization of the silk biomaterial. The functional domains may comprise receptor binding sites, enzymes, metal or sugar binding sites and others. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256055"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21256055/Optimization_of_a_retroviral_vector_for_transduction_of_human_CD34_positive_cells"><img alt="Research paper thumbnail of Optimization of a retroviral vector for transduction of human CD34 positive cells" class="work-thumbnail" src="https://attachments.academia-assets.com/41784732/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256055/Optimization_of_a_retroviral_vector_for_transduction_of_human_CD34_positive_cells">Optimization of a retroviral vector for transduction of human CD34 positive cells</a></div><div class="wp-workCard_item"><span>Acta biochimica Polonica</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human stem and progenitor cells have recently become objects of intensive studies as an important...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy and regenerative medicine. Retroviral vectors are among the most effective tools for genetic modification of these cells. However, their transduction efficiency strongly depends on the choice of the ex vivo transduction system. The aim of this study was to elaborate a system for retroviral vector transduction of human CD34 positive cells isolated from cord blood. The retroviral vector pMINV EGFP was chosen for transduction of two human erythroblastoid cell lines: KG-1a (CD34 positive) and K562 (CD34 negative). For vector construction, three promoters and two retroviral vector packaging cell lines were used. To optimize the physicochemical conditions of the transduction process, different temperatures of supernatant harvesting, the influence of centrifugation and the presence of transduction enhancing agents were tested. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21256054"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21256054/Expression_of_soluble_recombinant_TGF_beta_type_II_receptor_fused_with_the_Fc_portion_of_human_IgG1_sTbetaRII_Fc_in_NS0_cells"><img alt="Research paper thumbnail of Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells" class="work-thumbnail" src="https://attachments.academia-assets.com/41784737/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256054/Expression_of_soluble_recombinant_TGF_beta_type_II_receptor_fused_with_the_Fc_portion_of_human_IgG1_sTbetaRII_Fc_in_NS0_cells">Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells</a></div><div class="wp-workCard_item"><span>Acta biochimica Polonica</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused wi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTbetaRII-Fc binds native TGF-beta1 and TGF-beta3 isoforms and neutralizes their activity in vitro.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="84de84c10785f6abe3b27b4adacc98a8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784737,"asset_id":21256054,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784737/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256054"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256054"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256054; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256054]").text(description); $(".js-view-count[data-work-id=21256054]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256054; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256054']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256054, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "84de84c10785f6abe3b27b4adacc98a8" } } $('.js-work-strip[data-work-id=21256054]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256054,"title":"Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells","translated_title":"","metadata":{"abstract":"We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. 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cancer patients" class="work-thumbnail" src="https://attachments.academia-assets.com/41784736/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21256053/BRCA2_mutations_and_androgen_receptor_expression_as_independent_predictors_of_outcome_of_male_breast_cancer_patients">BRCA2 mutations and androgen receptor expression as independent predictors of outcome of male breast cancer patients</a></div><div class="wp-workCard_item"><span>Clinical cancer research : an official journal of the American Association for Cancer Research</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Germline mutations of the BRCA2 gene are involved in the development of a considerable number of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Germline mutations of the BRCA2 gene are involved in the development of a considerable number of male breast cancer cases. Although phenotypic differences have been observed between sporadic and BRCA-related breast carcinomas, conflicting data exist on the differences in prognosis of women with hereditary and sporadic breast cancer. The purpose of the study was to investigate the prognostic value of BRCA2 status in male breast carcinoma (MBC). We studied 43 male breast cancer patients, including 12 with BRCA2 mutations. Tumor samples were characterized immunohistochemically using antibodies to estrogen receptor, progesterone receptor, and androgen receptor (AR). BRCA2-related tumors presented at the earlier age compared with sporadic tumors (P = 0.005). Patients positive and negative for BRCA2 mutations did not differ with respect to tumor size, lymph node involvement, histological grade, and sex hormone receptor status. Five-year disease-free survival (DFS) and overall survival (OS...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2a59d5310ee1c2bdcd9d0ba8dffa53bf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41784736,"asset_id":21256053,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41784736/download_file?st=MTczMjQwNDA0NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21256053"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21256053"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21256053; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21256053]").text(description); $(".js-view-count[data-work-id=21256053]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21256053; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21256053']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 21256053, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2a59d5310ee1c2bdcd9d0ba8dffa53bf" } } $('.js-work-strip[data-work-id=21256053]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21256053,"title":"BRCA2 mutations and androgen receptor expression as independent predictors of outcome of male breast cancer patients","translated_title":"","metadata":{"abstract":"Germline mutations of the BRCA2 gene are involved in the development of a considerable number of male breast cancer cases. Although phenotypic differences have been observed between sporadic and BRCA-related breast carcinomas, conflicting data exist on the differences in prognosis of women with hereditary and sporadic breast cancer. The purpose of the study was to investigate the prognostic value of BRCA2 status in male breast carcinoma (MBC). We studied 43 male breast cancer patients, including 12 with BRCA2 mutations. Tumor samples were characterized immunohistochemically using antibodies to estrogen receptor, progesterone receptor, and androgen receptor (AR). BRCA2-related tumors presented at the earlier age compared with sporadic tumors (P = 0.005). Patients positive and negative for BRCA2 mutations did not differ with respect to tumor size, lymph node involvement, histological grade, and sex hormone receptor status. 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