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ENZYME - 1.14.13.246 4beta-methylsterol monooxygenase
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property="schema:alternateName">1.14.13.246</span></h1><div class="main-elmt" style="justify-self:center;"> <table class="type-1"> <tr><th colspan="2">Accepted Name</th></tr> <tr><td colspan="2"><strong property="schema:name">4beta-methylsterol monooxygenase </strong></td></tr> <tr><th colspan="2">Reaction catalysed</th></tr> <tr><td colspan="2">a 3beta-hydroxy-4,4-dimethylsteroid<span class="color_d2"> + </span>3 NADH<span class="color_d2"> + </span>3 O2<span class="color_d2"> + </span>2 H(+)<span class="color_d2"> = </span>a 3beta-hydroxy-4alpha-methylsteroid-4beta-carboxylate<span class="color_d2"> + </span>3 NAD(+)<span class="color_d2"> + </span>4 H2O</td></tr> <tr><th colspan="2">Comment(s)</th></tr> <tr><td colspan="2"><ul class="cc"><li>Contains a Rieske [2Fe-2S] iron-sulfur cluster. </li><li>This bacterial enzyme (SdmA) participates in the biosynthesis of bacterial sterols. </li><li>Together with SdmB it forms an enzyme system that removes one methyl group from the C-4 position of 4,4-dimethylated steroid molecules. </li><li>SdmA catalyzes three successive oxidations of the C-4beta methyl group, turning it into a carboxylate group; the second enzyme, SdmB, is a bifunctional enzyme that catalyzes two different activities. </li><li>As EC <a href="/EC/1.1.1.417">1.1.1.417</a> it catalyzes an oxidative decarboxylation that results in reduction of the 3beta-hydroxy group at the C-3 carbon to an oxo group. </li><li>As EC <a href="/EC/1.1.1.270">1.1.1.270</a> it reduces the 3-oxo group back to a 3beta-hydroxyl. </li><li>Unlike the animal/fungal enzyme EC <a href="/EC/1.14.18.9">1.14.18.9</a> and the plant enzymes EC <a href="/EC/1.14.18.10">1.14.18.10</a> and EC <a href="/EC/1.14.18.11">1.14.18.11</a>, this enzyme acts preferentially on the 4beta-methyl group. </li><li>Since no epimerization of the remaining C-4alpha methyl group occurs, the enzyme can only remove one methyl group, leaving a 4alpha- monomethylated product. </li></ul></td></tr> <tr><th colspan="2">Cross-references</th></tr> <tr><td>BRENDA</td><td><a href="http://www.brenda-enzymes.org/enzyme.php?ecno=1.14.13.246">1.14.13.246</a></td></tr> <tr><td>EC2PDB</td><td><a href="https://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/enzymes/GetPage.pl?ec_number=1.14.13.246">1.14.13.246</a></td></tr> <tr><td>ExplorEnz</td><td><a href="http://www.enzyme-database.org/query.php?ec=1.14.13.246">1.14.13.246</a></td></tr> <tr><td>PRIAM enzyme-specific profiles</td><td><a href="http://priam.prabi.fr/cgi-bin/PRIAM_profiles_CurrentRelease.pl?EC=1.14.13.246">1.14.13.246</a></td></tr> <tr><td>KEGG Ligand Database for Enzyme Nomenclature</td><td><a href="http://www.genome.ad.jp/dbget-bin/www_bget?ec:1.14.13.246">1.14.13.246</a></td></tr> <tr><td>IUBMB Enzyme Nomenclature</td><td><a href="https://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC1/14/13/246.html">1.14.13.246</a></td></tr> <tr><td>IntEnz</td><td><a href="https://www.ebi.ac.uk/intenz/query?cmd=SearchEC&ec=1.14.13.246">1.14.13.246</a></td></tr> <tr><td>MEDLINE</td><td><a href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=PureSearch&db=PubMed&details_term=1.14.13.246[EC/RN%20Number]">Find literature relating to 1.14.13.246</a></td></tr> <tr><td>MetaCyc</td><td><a href="https://biocyc.org/META/NEW-IMAGE?type=EC-NUMBER&object=EC-1.14.13.246">1.14.13.246</a></td></tr> <tr><td>Rhea expert-curated reactions</td><td><a href="https://www.rhea-db.org/rhea?query=ec%3A1.14.13.246">1.14.13.246</a></td></tr> <tr><td>UniProtKB/Swiss-Prot</td><td><table> <tr><td><a href="https://www.uniprot.org/uniprot/Q60A55">Q60A55</a>, SDMA_METCA</td></table> </td></tr> </table> <br><a property="schema:sameAs" href="/cgi-bin/enzyme/get-enzyme-entry?1.14.13.246">View entry in original ENZYME format</a> <br><a property="schema:sameAs" href="/EC/1.14.13.246.txt">View entry in raw text format (no links)</a><br> <div property="schema:about" typeof="schema:DataSet"><a property="schema:url" href="https://www.uniprot.org/uniprotkb?query=(ec:1.14.13.246)%20AND%20(reviewed:true)">All UniProtKB/Swiss-Prot entries referenced in this entry</a>, with possibility to download in different formats, align etc. </div> All <a href="/EC/1.14.13.-">ENZYME</a> / <a href="https://www.uniprot.org/uniprotkb?query=(ec:1.14.13.-)%20AND%20(reviewed:true)">UniProtKB/Swiss-Prot</a> entries corresponding to 1.14.13.-<br> </span><span property="itemListElement" typeof="ListItem">All <a href="/EC/1.14.-.-">ENZYME</a> / <a href="https://www.uniprot.org/uniprotkb?query=(ec:1.14.-.-)%20AND%20(reviewed:true)">UniProtKB/Swiss-Prot</a> entries corresponding to 1.14.-.-<br> All <a href="/EC/1.-.-.-">ENZYME</a> / <a href="https://www.uniprot.org/uniprotkb?query=(ec:1.-.-.-)%20AND%20(reviewed:true)">UniProtKB/Swiss-Prot</a> entries corresponding to 1.-.-.-<br> </div> </div> </main> <!-- end of main, start of footer --> <footer class="footer-grid"> <div 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