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Silva</title> <meta name="description" content="Search results for: Elisa Silva"> <meta name="keywords" content="Elisa Silva"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Elisa Silva"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 787</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Elisa Silva</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">787</span> Novel Nanomagnetic Beads Based- Latex Agglutination Assay for Rapid Diagnosis of Human Schistosomiasis Haematobium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20Aly">Ibrahim Aly</a>, <a href="https://publications.waset.org/abstracts/search?q=Rabab%20Zalat"> Rabab Zalat</a>, <a href="https://publications.waset.org/abstracts/search?q=Bahaa%20EL%20Deen%20W.%20El%20Aswad"> Bahaa EL Deen W. El Aswad</a>, <a href="https://publications.waset.org/abstracts/search?q=Ismail%20M.%20Moharm"> Ismail M. Moharm</a>, <a href="https://publications.waset.org/abstracts/search?q=Basam%20M.%20Masoud"> Basam M. Masoud</a>, <a href="https://publications.waset.org/abstracts/search?q=Tarek%20Diab"> Tarek Diab</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of the present study was to evaluate the novel nanomagnetic beads based–latex agglutination assay (NMB-LAT) as a simple test for diagnosis of S. haematobium as well as standardize the novel nanomagnetic beads based –ELISA (NMB-ELISA). According to urine examination this study included 85 S. haematobium infected patients, 30 other parasites infected patients and 25 negative control samples. The sensitivity of novel NMB-LAT was 82.4% versus 96.5% and 88.2% for NMB-ELISA and currently used sandwich ELISA respectively. The specificity of NMB-LAT was 83.6% versus 96.3% and 87.3% for NMB-ELISA and currently used sandwich ELISA respectively. In conclusion, the novel NMB-ELISA is a valuable applicable diagnostic technique for diagnosis of human schistosomiasis haematobium. The novel NMB-ELISA assay is a suitable applicable diagnostic method in field survey especially when followed by ELISA as a confirmatory test in query false negative results. Trials are required to increase the sensitivity and specificity of NMB-ELISA assay. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title="diagnosis">diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=iatex%20agglutination" title=" iatex agglutination"> iatex agglutination</a>, <a href="https://publications.waset.org/abstracts/search?q=nanomagnetic%20beads" title=" nanomagnetic beads"> nanomagnetic beads</a>, <a href="https://publications.waset.org/abstracts/search?q=sandwich%20ELISA" title=" sandwich ELISA"> sandwich ELISA</a> </p> <a href="https://publications.waset.org/abstracts/2898/novel-nanomagnetic-beads-based-latex-agglutination-assay-for-rapid-diagnosis-of-human-schistosomiasis-haematobium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2898.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">382</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">786</span> The Differences between Direct Examination and ELISA Test during the Diagnosis of Fasciolosis in Jaundiced Slaughtered Sheep in Iraq</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Azad%20A.%20Meerkhan">Azad A. Meerkhan</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20Hani%20Razak"> Alaa Hani Razak</a>, <a href="https://publications.waset.org/abstracts/search?q=Bayan%20M.%20S.%20Younis"> Bayan M. S. Younis</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The efficiency of enzyme-linked immunosorbent assay (ELISA) in sheep infected with Fasciola hepatica was studied. 232 jaundiced sheep among 5208 sheep slaughter in the Duhok abattoir (regardless of the age and gender) between the period of May. 2012 to Oct. 2012 were examined by direct examination (Searching of adult ﬂukes in the bile duct) and by Enzyme-linked immunosorbent assay (ELISA) to detect the prevalence of fascioliasis in the studied population which showed a high observed infection ratio in Sep. 2012 (12.2%) with the high (ELISA) result of infection in May. 2012 (25.36%). Significant differences were found between the two ways in all of the months with the highest difference in May. 2012 and the net deference between the both ways was 6.91%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fascioliasis" title="fascioliasis">fascioliasis</a>, <a href="https://publications.waset.org/abstracts/search?q=Fasciola%20hepatica" title=" Fasciola hepatica"> Fasciola hepatica</a>, <a href="https://publications.waset.org/abstracts/search?q=layers" title=" layers"> layers</a>, <a href="https://publications.waset.org/abstracts/search?q=liver%20fluk" title=" liver fluk"> liver fluk</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=direct%20examination" title=" direct examination"> direct examination</a> </p> <a href="https://publications.waset.org/abstracts/1910/the-differences-between-direct-examination-and-elisa-test-during-the-diagnosis-of-fasciolosis-in-jaundiced-slaughtered-sheep-in-iraq" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1910.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">322</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">785</span> A Field Study of Monochromatic Light Effects on Antibody Responses to Newcastle Disease by HI Test and the Correlation with ELISA</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seyed%20Mehrzad%20Pahlavani">Seyed Mehrzad Pahlavani</a>, <a href="https://publications.waset.org/abstracts/search?q=Mozaffar%20Haji%20Jafari%20Anaraki"> Mozaffar Haji Jafari Anaraki</a>, <a href="https://publications.waset.org/abstracts/search?q=Sayma%20Mohammadi"> Sayma Mohammadi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A total of 34700 day-old broilers were exposed to green, blue and yellow light using a light-emitting diode system for 6 weeks to investigate the effects of light wave length on antibody responses to Newcastle disease by HI test and the correlation with ELISA. 3 poultry house broiler farms with the same conditions was selected and the lightening system of each was set according to the requirement. Blood samples were taken from 20 chicks on days 1, 24 and 46 and the Newcastle virus specific antibody was titered in serum using HI an ELISA test. On day 24, the probability value of more than 0/05 was observed in HI and ELISA tests of all groups while at the end of breeding period, the average HI serum antibody titer was more in the green light than the yellow one while the blue light was not significantly different from both. At the last titration, the green light has got the highest titer of Newcastle antibodies. There were no significant differences of Newcastle antibody titers between all groups and ages in broiler pullets in ELISA. According to the sampling and analysis of HI and ELISA serum tests, there were no significant relationships between all broiler pullets breeding in green, blue and yellow light on days 24 and 46 and the P-value was more than 0/05. It is suggested that the monochromatic light is effective on broilers immunity against Newcastle disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=monochromatic%20light" title="monochromatic light">monochromatic light</a>, <a href="https://publications.waset.org/abstracts/search?q=Newcastle%20disease" title=" Newcastle disease"> Newcastle disease</a>, <a href="https://publications.waset.org/abstracts/search?q=HI%20test" title=" HI test"> HI test</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA%20test" title=" ELISA test"> ELISA test</a> </p> <a href="https://publications.waset.org/abstracts/6039/a-field-study-of-monochromatic-light-effects-on-antibody-responses-to-newcastle-disease-by-hi-test-and-the-correlation-with-elisa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/6039.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">657</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">784</span> Diagnosis of Rotavirus Infection among Egyptian Children by Using Different Laboratory Techniques</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20A.%20Alhammad">Mohamed A. Alhammad</a>, <a href="https://publications.waset.org/abstracts/search?q=Hadia%20A.%20Abou-Donia"> Hadia A. Abou-Donia</a>, <a href="https://publications.waset.org/abstracts/search?q=Mona%20H.%20Hashish"> Mona H. Hashish</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20N.%20Massoud"> Mohamed N. Massoud</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Rotavirus is the leading etiologic agent of severe diarrheal disease in infants and young children worldwide. The present study was aimed 1) to detect rotavirus infection as a cause of diarrhoea among children under 5 years of age using the two serological methods (ELISA and LA) and the PCR technique (2) to evaluate the three methodologies used for human RV detection in stool samples. Materials and Methods: This study was carried out on 247 children less than 5 years old, diagnosed clinically as acute gastroenteritis and attending Alexandria University Children Hospital at EL-Shatby. Rotavirus antigen was screened by ELISA and LA tests in all stool samples, whereas only 100 samples were subjected to RT-PCR method for detection of rotavirus RNA. Results: Out of the 247 studied cases with diarrhoea, rotavirus antigen was detected in 83 (33.6%) by ELISA and 73 (29.6%) by LA, while the 100 cases tested by RT-PCR showed that 44% of them had rotavirus RNA. Rotavirus diarrhoea was significantly presented with a marked seasonal peak during autumn and winter (61.4%). Conclusion: The present study confirms the huge burden of rotavirus as a major cause of acute diarrhoea in Egyptian infants and young children. It was concluded that; LA is equal in sensitivity to ELISA, ELISA is more specific than LA, and RT-PCR is more specific than ELISA and LA in diagnosis of rotavirus infection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rotavirus" title="rotavirus">rotavirus</a>, <a href="https://publications.waset.org/abstracts/search?q=diarrhea" title=" diarrhea"> diarrhea</a>, <a href="https://publications.waset.org/abstracts/search?q=immunoenzyme%20techniques" title=" immunoenzyme techniques"> immunoenzyme techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=latex%20fixation%20tests" title=" latex fixation tests"> latex fixation tests</a>, <a href="https://publications.waset.org/abstracts/search?q=RT-PCR" title=" RT-PCR"> RT-PCR</a> </p> <a href="https://publications.waset.org/abstracts/21006/diagnosis-of-rotavirus-infection-among-egyptian-children-by-using-different-laboratory-techniques" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21006.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">370</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">783</span> Detection of Helicobacter Pylori by PCR and ELISA Methods in Patients with Hyperlipidemia </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Simin%20Khodabakhshi">Simin Khodabakhshi</a>, <a href="https://publications.waset.org/abstracts/search?q=Hossein%20Rassi"> Hossein Rassi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hyperlipidemia refers to any of several acquired or genetic disorders that result in a high level of lipids circulating in the blood. Helicobacter pylori infection is a contributing factor in the progression of hyperlipidemia with serum lipid changes. The aim of this study was to detect of Helicobacter pylori by PCR and serological methods in patients with hyperlipidemia. In this case-control study, 174 patients with hyperlipidemia and 174 healthy controls were studied. Also, demographics, physical and biochemical parameters were performed in all samples. The DNA extracted from blood specimens was amplified by H pylori cagA specific primers. The results show that H. pylori cagA positivity was detected in 79% of the hyperlipidemia and in 56% of the control group by ELISA test and 49% of the hyperlipidemia and in 24% of the control group by PCR test. Prevalence of H. pylori infection was significantly higher in hyperlipidemia as compared to controls. In addition, patients with hyperlipidemia had significantly higher values for triglyceride, total cholesterol, LDL-C, waist to hip ratio, body mass index, diastolic and systolic blood pressure and lower levels of HDL-C than control participants (all p < 0.0001). Our result detected the ELISA was a rapid and cost-effective detection and considering the high prevalence of cytotoxigenic H. pylori strains, cag A is suggested as a promising target for PCR and ELISA tests for detection of infection with toxigenic strains. In general, it can be concluded that molecular analysis of H. pylori cagA and clinical parameters are important in early detection of hyperlipidemia and atherosclerosis with H. pylori infection by PCR and ELISA tests. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Helicobacter%20pylori" title="Helicobacter pylori">Helicobacter pylori</a>, <a href="https://publications.waset.org/abstracts/search?q=hyperlipidemia" title=" hyperlipidemia"> hyperlipidemia</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA "> ELISA </a> </p> <a href="https://publications.waset.org/abstracts/85468/detection-of-helicobacter-pylori-by-pcr-and-elisa-methods-in-patients-with-hyperlipidemia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/85468.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">199</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">782</span> Nano-Immunoassay for Diagnosis of Active Schistosomal Infection </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Manal%20M.%20Kame">Manal M. Kame</a>, <a href="https://publications.waset.org/abstracts/search?q=Hanan%20G.%20El-Baz"> Hanan G. El-Baz</a>, <a href="https://publications.waset.org/abstracts/search?q=Zeinab%20A.Demerdash"> Zeinab A.Demerdash</a>, <a href="https://publications.waset.org/abstracts/search?q=Engy%20M.%20Abd%20El-Moneem"> Engy M. Abd El-Moneem</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20A.%20Hendawy"> Mohamed A. Hendawy</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20R.%20Bayoumi"> Ibrahim R. Bayoumi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is a constant need to improve the performance of current diagnostic assays of schistosomiasis as well as develop innovative testing strategies to meet new testing challenges. This study aims at increasing the diagnostic efficiency of monoclonal antibody (MAb)-based antigen detection assays through gold nanoparticles conjugated with specific anti-Schistosoma mansoni monoclonal antibodies. In this study, several hybidoma cell lines secreting MAbs against adult worm tegumental Schistosoma antigen (AWTA) were produced at Immunology Department of Theodor Bilharz Research Institute and preserved in liquid nitrogen. One MAb (6D/6F) was chosen for this study due to its high reactivity to schistosome antigens with highest optical density (OD) values. Gold nanoparticles (AuNPs) were functionalized and conjugated with MAb (6D/6F). The study was conducted on serum samples of 116 subjects: 71 patients with S. mansoni eggs in their stool samples group (gp 1), 25 with other parasites (gp2) and 20 negative healthy controls (gp3). Patients in gp1 were further subdivided according to egg count in their stool samples into Light infection {≤ 50 egg per gram(epg) (n= 17)}, moderate {51-100 epg (n= 33)} and severe infection {>100 epg(n= 21)}. Sandwich ELISA was performed using (AuNPs -MAb) for detection of circulating schistosomal antigen (CSA) levels in serum samples of all groups and the results were compared with that after using MAb/ sandwich ELISA system. Results Gold- MAb/ ELISA system reached a lower detection limit of 10 ng/ml compared to 85 ng/ml on using MAb/ ELISA and the optimal concentrations of AuNPs -MAb were found to be 12 folds less than that of MAb/ ELISA system for detection of CSA. The sensitivity and specificity of sandwich ELISA for detection of CSA levels using AuNPs -MAb were 100% & 97.8 % respectively compared to 87.3% &93.38% respectively on using MAb/ ELISA system. It was found that CSA was detected in 9 out of 71 S.mansoni infected patients on using AuNPs - MAb/ ELISA system and was not detected by MAb/ ELISA system. All those patients (9) was found to have an egg count below 50 epg feces (patients with light infections). ROC curve analyses revealed that sandwich ELISA using gold-MAb was an excellent diagnostic investigator that could differentiate Schistosoma patients from healthy controls, on the other hand it revealed that sandwich ELISA using MAb was not accurate enough as it could not recognize nine out of 71 patients with light infections. Conclusion Our data demonstrated that: Loading gold nanoparticles with MAb (6D/6F) increases the sensitivity and specificity of sandwich ELISA for detection of CSA, thus active (early) and light infections could be easily detected. Moreover this binding will decrease the amount of MAb consumed in the assay and lower the coast. The significant positive correlation that was detected between ova count (intensity of infection) and OD reading in sandwich ELISA using gold- MAb enables its use to detect the severity of infections and follow up patients after treatment for monitoring of cure. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Schistosomiasis" title="Schistosomiasis">Schistosomiasis</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=gold" title=" gold"> gold</a>, <a href="https://publications.waset.org/abstracts/search?q=monoclonal%20antibodies" title=" monoclonal antibodies"> monoclonal antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA "> ELISA </a> </p> <a href="https://publications.waset.org/abstracts/15512/nano-immunoassay-for-diagnosis-of-active-schistosomal-infection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15512.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">371</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">781</span> Detection of Total Aflatoxin in Flour of Wheat and Maize Samples in Albania Using ELISA</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aferdita%20Dinaku">Aferdita Dinaku</a>, <a href="https://publications.waset.org/abstracts/search?q=Jonida%20Canaj"> Jonida Canaj</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aflatoxins are potentially toxic metabolites produced by certain kinds of fungi (molds) that are found naturally all over the world; they can contaminate food crops and pose a serious health threat to humans by mutagenic and carcinogenic effects. Several types of aflatoxin (14 or more) occur in nature. In Albanian nutrition, cereals (especially wheat and corn) are common ingredients in some traditional meals. This study aimed to investigate the presence of aflatoxins in the flour of wheat and maize that are consumed in Albania’s markets. The samples were collected randomly in different markets in Albania and detected by the ELISA method, measured in 450 nm. The concentration of total aflatoxins was analyzed by enzyme-linked immunosorbent assay (ELISA), and they were ranged between 0.05-1.09 ppb. However, the screened mycotoxin levels in the samples were lower than the maximum permissible limits of European Commission No 1881/2006 (4 μg/kg). The linearity of calibration curves was good for total aflatoxins (B1, B2, G1, G2, M1) (R²=0.99) in the concentration range 0.005-4.05 ppb. The samples were analyzed in two replicated measurements and for each sample, the standard deviation (statistical parameter) is calculated. The results showed that the flour samples are safe, but the necessity of performing such tests is necessary. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aflatoxins" title="aflatoxins">aflatoxins</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA%20technique" title=" ELISA technique"> ELISA technique</a>, <a href="https://publications.waset.org/abstracts/search?q=food%20contamination" title=" food contamination"> food contamination</a>, <a href="https://publications.waset.org/abstracts/search?q=flour" title=" flour"> flour</a> </p> <a href="https://publications.waset.org/abstracts/132620/detection-of-total-aflatoxin-in-flour-of-wheat-and-maize-samples-in-albania-using-elisa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/132620.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">157</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">780</span> Comparison of Aflatoxin B1 Levels in Iranian and Indian Spices by ELISA Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amir%20Sasan%20Mozaffari%20Nejad">Amir Sasan Mozaffari Nejad </a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study was carried out to detect the presence of aflatoxin B1 (AFB1) in 36 samples of spices from Iran and India that was included of chilli powder (n=12), black pepper powder (n=12) and whole black pepper (n=12). Enzyme-linked immunosorbent assay (ELISA) method was used for analysing the samples. Aflatoxin B1 was found in all the spices samples, the concentration of AFB1 in Iranian samples was ranged from 63.16 to 626.81 ng/kg and in Indian samples was ranged from 31.15 to 245.94 ng/kg. The mean of AFB1 concentration in the chilli powder was significantly higher (P < 0.05) than the whole and powdered black pepper. However, none of the samples exceeded the maximum prescribed limit i.e. 5 µg/kg of European Union regulations for aflatoxin B1. The occurrence of AFB1 in spices samples could be a potential hazard for public health. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aflatoxin%20B1" title="Aflatoxin B1">Aflatoxin B1</a>, <a href="https://publications.waset.org/abstracts/search?q=chilli" title=" chilli"> chilli</a>, <a href="https://publications.waset.org/abstracts/search?q=black%20pepper" title=" black pepper"> black pepper</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=Iran" title=" Iran"> Iran</a>, <a href="https://publications.waset.org/abstracts/search?q=India" title=" India"> India</a> </p> <a href="https://publications.waset.org/abstracts/2110/comparison-of-aflatoxin-b1-levels-in-iranian-and-indian-spices-by-elisa-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2110.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">441</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">779</span> Enhancing the Sensitivity of Antigen Based Sandwich ELISA for COVID-19 Diagnosis in Saliva Using Gold Conjugated Nanobodies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Manal%20Kamel">Manal Kamel</a>, <a href="https://publications.waset.org/abstracts/search?q=Sara%20Maher"> Sara Maher</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Development of sensitive non-invasive tests for detection of SARS-CoV-2 antigens is imperative to manage the extent of infection throughout the population, yet, it is still challenging. Here, we designed and optimized a sandwich enzyme-linked immunosorbent assay (ELISA) for SARS-CoV-2 S1 antigen detection in saliva. Both saliva samples and nasopharyngeal swapswere collected from 170 PCR-confirmed positive and negative cases. Gold nanoparticles (AuNPs) were conjugated with S1protein receptor binding domain (RBD) nanobodies. Recombinant S1 monoclonal antibodies (S1mAb) as primery antibody and gold conjugated nanobodies as secondary antibody were employed in sandwich ELISA. Our developed system were optimized to achieve 87.5 % sensitivity and 100% specificity for saliva samples compared to 89 % and 100% for nasopharyngeal swaps, respectively. This means that saliva could be a suitable replacement for nasopharyngeal swaps No cross reaction was detected with other corona virus antigens. These results revealed that our developed ELISAcould be establishedas a new, reliable, sensitive, and non-invasive test for diagnosis of SARS-CoV-2 infection, using the easily collected saliva samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=COVID%2019" title="COVID 19">COVID 19</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=nanobodies" title=" nanobodies"> nanobodies</a> </p> <a href="https://publications.waset.org/abstracts/148038/enhancing-the-sensitivity-of-antigen-based-sandwich-elisa-for-covid-19-diagnosis-in-saliva-using-gold-conjugated-nanobodies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/148038.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">134</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">778</span> A Novel Nano-Chip Card Assay as Rapid Test for Diagnosis of Lymphatic Filariasis Compared to Nano-Based Enzyme Linked Immunosorbent Assay</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20Aly">Ibrahim Aly</a>, <a href="https://publications.waset.org/abstracts/search?q=Manal%20Ahmed"> Manal Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20M.%20El-Shall"> Mahmoud M. El-Shall</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Filariasis is a parasitic disease caused by small roundworms. The filarial worms are transmitted and spread by blood-feeding black flies and mosquitoes. Lymphatic filariasis (Elephantiasis) is caused by Wuchereriabancrofti, Brugiamalayi, and Brugiatimori. Elimination of Lymphatic filariasis necessitates an increasing demand for valid, reliable, and rapid diagnostic kits. Nanodiagnostics involve the use of nanotechnology in clinical diagnosis to meet the demands for increased sensitivity, specificity, and early detection in less time. The aim of this study was to evaluate the nano-based enzymelinked immunosorbent assay (ELISA) and novel nano-chip card as a rapid test for detection of filarial antigen in serum samples of human filariasis in comparison with traditional -ELISA. Serum samples were collected from an infected human with filarial gathered across Egypt's governorates. After receiving informed consenta total of 45 blood samples of infected individuals residing in different villages in Gharbea governorate, which isa nonendemic region for bancroftianfilariasis, healthy persons living in nonendemic locations (20 persons), as well as sera from 20 other parasites, affected patients were collected. The microfilaria was checked in thick smears of 20 µl night blood samples collected during 20-22 hrs. All of these individuals underwent the following procedures: history taking, clinical examination, and laboratory investigations, which included examination of blood samples for microfilaria using thick blood film and serological tests for detection of the circulating filarial antigen using polyclonal antibody- ELISA, nano-based ELISA, and nano-chip card. In the present study, a recently reported polyoclonal antibody specific to tegumental filarial antigen was used in developing nano-chip card and nano-ELISA compared to traditional ELISA for the detection of circulating filarial antigen in sera of patients with bancroftianfilariasis. The performance of the ELISA was evaluated using 45 serum samples. The ELISA was positive with sera from microfilaremicbancroftianfilariasis patients (n = 36) with a sensitivity of 80 %. Circulating filarial antigen was detected in 39/45 patients who were positive for circulating filarial antigen using nano-ELISA with a sensitivity of 86.6 %. On the other hand, 42 out of 45 patients were positive for circulating filarial antigen using nano-chip card with a sensitivity of 93.3%.In conclusion, using a novel nano-chip assay could potentially be a promising alternative antigen detection test for bancroftianfilariasis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lymphatic%20filariasis" title="lymphatic filariasis">lymphatic filariasis</a>, <a href="https://publications.waset.org/abstracts/search?q=nanotechnology" title=" nanotechnology"> nanotechnology</a>, <a href="https://publications.waset.org/abstracts/search?q=rapid%20diagnosis" title=" rapid diagnosis"> rapid diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=elisa%20technique" title=" elisa technique"> elisa technique</a> </p> <a href="https://publications.waset.org/abstracts/149798/a-novel-nano-chip-card-assay-as-rapid-test-for-diagnosis-of-lymphatic-filariasis-compared-to-nano-based-enzyme-linked-immunosorbent-assay" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149798.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">115</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">777</span> Serological Screening of Barrier Maintained Rodent Colony</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=R.%20Posia">R. Posia</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Mistry"> J. Mistry</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Kamani"> K. Kamani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The health screening of laboratory rodents is essential for ensuring animal health and the validity of biomedical research data. Routine health monitoring is necessary to verify the effectiveness of biosecurity and the specific pathogen free (SPF) status of the colony. The present screening was performed in barrier maintained rat (Rattus norvegicus) colony. Rats were maintained under a controlled environment and strict biosecurity in the facility. The screening was performed on quarterly bases from randomly selected animals from breeding and or maintenance colonies. Selected animals were subject to blood collection under isoflurane anaesthesia. Serum was separated from the collected blood and stored samples at -60 ± 10 °C until further use. A total of 88 samples were collected quarterly bases from animals in a year. In the serological test, enzyme-linked immunosorbent assay (ELISA) was used for screening of serum samples against sialodacryoadenitis virus (SDAV), Sendai virus (SV), and Kilham’s rat virus (KRV). ELISA kits were procured from XpressBio, USA. Test serum samples were run along with positive control, negative control serum in 96 well ELISA plates as per the procedure recommended by the vendor. Test ELISA plate reading was taken in the microplate reader. This screening observed that none of the samples was observed positive for the sialodacryoadenitis virus (SDAV), Sendai virus (SV), and Kilham’s rat virus (KRV), indicating that effectiveness of biosecurity practices followed in the rodent colony. The result of serological screening helps us to declare that our rodent colony is specifically pathogen free for these pathogens. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosecurity" title="biosecurity">biosecurity</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=specific%20pathogen%20free" title=" specific pathogen free"> specific pathogen free</a>, <a href="https://publications.waset.org/abstracts/search?q=serological%20screening" title=" serological screening"> serological screening</a>, <a href="https://publications.waset.org/abstracts/search?q=serum" title=" serum"> serum</a> </p> <a href="https://publications.waset.org/abstracts/163395/serological-screening-of-barrier-maintained-rodent-colony" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163395.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">77</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">776</span> RF Propagation Analysis in Outdoor Environments Using RSSI Measurements Applied in ZigBee Sensor Networks</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Teles%20de%20Sales%20Bezerra">Teles de Sales Bezerra</a>, <a href="https://publications.waset.org/abstracts/search?q=Saulo%20Aislan%20da%20Silva%20Eleuterio"> Saulo Aislan da Silva Eleuterio</a>, <a href="https://publications.waset.org/abstracts/search?q=Jos%C3%A9%20Anderson%20Rodrigues%20de%20Souza"> José Anderson Rodrigues de Souza</a>, <a href="https://publications.waset.org/abstracts/search?q=Jeronimo%20Silva%20Rocha"> Jeronimo Silva Rocha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Propagation in radio frequency is a constant concern in the application of Wireless Sensor Networks (WSN), the behavior of an environment determines how good the quality of signal reception. The objective of this paper is to analyze the behavior of a WSN in an environment for agriculture where environmental variables are present and correlate the capture of values received signal strength (RSSI) with a propagation model. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=propagation" title="propagation">propagation</a>, <a href="https://publications.waset.org/abstracts/search?q=WSN" title=" WSN"> WSN</a>, <a href="https://publications.waset.org/abstracts/search?q=agriculture" title=" agriculture"> agriculture</a>, <a href="https://publications.waset.org/abstracts/search?q=quality" title=" quality"> quality</a> </p> <a href="https://publications.waset.org/abstracts/20471/rf-propagation-analysis-in-outdoor-environments-using-rssi-measurements-applied-in-zigbee-sensor-networks" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20471.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">755</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">775</span> The Arabian Camel (Camelus dromedarius) as a Major Reservoir of Q Fever in Saudi Arabia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mansour%20F.%20Hussein">Mansour F. Hussein</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20A.%20Alshaikh"> Mohammed A. Alshaikh</a>, <a href="https://publications.waset.org/abstracts/search?q=Riyadh%20S.%20Al-Jumaah"> Riyadh S. Al-Jumaah</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20GarelNabi"> A. GarelNabi</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Al-Khalifa"> I. Al-Khalifa</a>, <a href="https://publications.waset.org/abstracts/search?q=Osama%20B.%20Mohammed"> Osama B. Mohammed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Serum samples from 489 male and female camels were tested for antibodies against C. burnetii using indirect enzyme-linked immunosorbent assay (ELISA). Antibodies to C. burnetii were recorded in sera of 252 (51.64%) camels. Significant differences in prevalence were found between male and female camels, juvenile and adult camels, different ecotypes and different sampling locations. 307 camels were simultaneously tested for C. burnetii antibodies by ELISA and indirect immunofluorescence (IFA). Close agreement was found between the results of the two tests. A high prevalence of C. burnetii antibodies was also recorded in milk samples tested by ELISA. Clinical samples from serologically positive camels were subjected to PCR analysis using primers which amplify the repetitive transposon-like and transposase gene regions of C. burnetii. Positive DNA amplification was obtained from both regions, with highest shedding of C. burnetii in faecal samples (27.59%) followed, in descending order, by urine (23.81%), blood (15.85%) and milk (6.5%). The present results indicate that camels are a major reservoir of C. burnetii in Saudi Arabia. The high prevalence of infection in camels, the poor sanitary standards under which the animals are kept and the consumption of raw camel milk indicate that camels could also be a major source of transmission of Q fever to humans in Saudi Arabia. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arabian%20camel" title="Arabian camel">Arabian camel</a>, <a href="https://publications.waset.org/abstracts/search?q=Camelus%20dromedarius" title=" Camelus dromedarius"> Camelus dromedarius</a>, <a href="https://publications.waset.org/abstracts/search?q=Coxiella%20brunetii" title=" Coxiella brunetii"> Coxiella brunetii</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=immunofluoresence" title=" immunofluoresence"> immunofluoresence</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a> </p> <a href="https://publications.waset.org/abstracts/8944/the-arabian-camel-camelus-dromedarius-as-a-major-reservoir-of-q-fever-in-saudi-arabia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/8944.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">653</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">774</span> Determination of Some Etiologic Agents in Calves with Diarrhea</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nermin%20Isik">Nermin Isik</a>, <a href="https://publications.waset.org/abstracts/search?q=Ozlem%20Derinbay%20Ekici"> Ozlem Derinbay Ekici</a>, <a href="https://publications.waset.org/abstracts/search?q=Oguzhan%20Avci"> Oguzhan Avci</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study was to determination of role infection in neonatal calves in Central Anatolian, Turkey. A total 300 fecal samples were collected from diarrheic neonatal calves, aged between 0–90 days from Konya, Karaman, and Aksaray from January to April 2014. Fecal specimens from calves with clinically diarrheic symptoms were examined for the presence of Bovine Coronavirus, Bovine Rotavirus, Cryptosporidium sp., and E. coli by commercially available capture direct enzyme linked immunosorbent assay (ELISA) kit and Modified Ziehl Neelsen method (MZN). Calves were grouped according to their age as follows: 1-14, 15-29, and 30-90 days. Cryptosporidium sp. infection was detected in 52.8%, 58.8%, and 39.2% by ELISA and 33.9%, 47%, 26.7% by MZN in the respective age groups. The seroprevalance of Rotavirus (12.5 %, 40 %, 12.5 %), Coronavirus (2.5%, 0%, 3.5%) and E. coli (5%, 4.7%, 8.9%) infections were determined according to the age groups respectively. Cryptosporidium sp. was the most detected enteropathogen (52 %) of calves and coronavirus was the least detected (2 %). The detection rate of the mixed enfection was 12.3%. In conclusion, it must be evaluated by mix infections in calves with diarrhea. These results will provide an important contribution against the factors that cause diarrhea <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cryptosporidium%20sp." title="cryptosporidium sp.">cryptosporidium sp.</a>, <a href="https://publications.waset.org/abstracts/search?q=bovine%20coronavirus" title=" bovine coronavirus"> bovine coronavirus</a>, <a href="https://publications.waset.org/abstracts/search?q=bovine%20rotavirus" title=" bovine rotavirus"> bovine rotavirus</a>, <a href="https://publications.waset.org/abstracts/search?q=E.coli" title=" E.coli"> E.coli</a>, <a href="https://publications.waset.org/abstracts/search?q=calve" title=" calve"> calve</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a> </p> <a href="https://publications.waset.org/abstracts/27516/determination-of-some-etiologic-agents-in-calves-with-diarrhea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27516.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">552</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">773</span> A Proposal of Ontology about Brazilian Government Transparency Portal</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Estela%20Mayra%20de%20Moura%20Vianna">Estela Mayra de Moura Vianna</a>, <a href="https://publications.waset.org/abstracts/search?q=Thiago%20Jos%C3%A9%20Tavares%20%C3%81vila"> Thiago José Tavares Ávila</a>, <a href="https://publications.waset.org/abstracts/search?q=Bruno%20Morais%20Silva"> Bruno Morais Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Diego%20Henrique%20Bezerra"> Diego Henrique Bezerra</a>, <a href="https://publications.waset.org/abstracts/search?q=Paulo%20Henrique%20Gomes%20Silva"> Paulo Henrique Gomes Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Alan%20Pedro%20da%20Silva"> Alan Pedro da Silva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Brazilian Federal Constitution defines the access to information as a crucial right of the citizen and the Law on Access to Public Information, which regulates this right. Accordingly, the Fiscal Responsibility Act, 2000, amended in 2009 by the “Law of Transparency”, began demanding a wider disclosure of public accounts for the society, including electronic media for public access. Thus, public entities began to create "Transparency Portals," which aim to gather a diversity of data and information. However, this information, in general, is still published in formats that do not simplify understanding of the data by citizens and that could be better especially available for audit purposes. In this context, a proposal of ontology about Brazilian Transparency Portal can play a key role in how these data will be better available. This study aims to identify and implement in ontology, the data model about Transparency Portal ecosystem, with emphasis in activities that use these data for some applications, like audits, press activities, social government control, and others. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=audit" title="audit">audit</a>, <a href="https://publications.waset.org/abstracts/search?q=government%20transparency" title=" government transparency"> government transparency</a>, <a href="https://publications.waset.org/abstracts/search?q=ontology" title=" ontology"> ontology</a>, <a href="https://publications.waset.org/abstracts/search?q=public%20sector" title=" public sector"> public sector</a> </p> <a href="https://publications.waset.org/abstracts/14657/a-proposal-of-ontology-about-brazilian-government-transparency-portal" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14657.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">506</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">772</span> Hepatitis E among Pregnant Women in Urmia, Iran</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakieh%20Rostamzadeh%20Khameneh">Zakieh Rostamzadeh Khameneh</a>, <a href="https://publications.waset.org/abstracts/search?q=Nariman%20Sepehrvand"> Nariman Sepehrvand</a>, <a href="https://publications.waset.org/abstracts/search?q=Khalkhali-Zahra%20Shirmohamadi"> Khalkhali-Zahra Shirmohamadi </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Although the hepatitis E virus mostly causes a self-limited disease in general population, the disease is more severe in pregnant women. Hepatitis E accounts for about 10% of pregnancy-associated deaths in southern Asia. Methods: 136 pregnant women who referred to urban health centers of Urmia for pursuing pregnancy-related health services were selected randomly and enrolled in a descriptive, cross-sectional study. Each subject was tested for the presence of anti-HEV IgG antibody using an enzyme-linked immunosorbent assay (ELISA, Dia.Pro). Results: The mean age among 136 pregnant women was 25.12±4.91 years old (range of 14-39 years). Only five cases (3.6%) among all 136 subjects were demonstrated to be seropositive for anti-HEV IgG using ELISA method. There was no significant difference between age (P=0.88), income level (P=0.19) of two seropositive and seronegative groups. All seropositive cases were from urban areas. Conclusion: The seroprevalence of anti-HEV IgG is low in the population of pregnant women in Urmia, Iran. Because of limited sample size in this study, we recommend to perform further studies with larger sample size in other regions of Iran in order to be able to systematically generalize the findings of studies to the population of Iranian pregnant women. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pregnancy" title="pregnancy">pregnancy</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatitis%20E" title=" hepatitis E"> hepatitis E</a>, <a href="https://publications.waset.org/abstracts/search?q=women" title=" women"> women</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA "> ELISA </a> </p> <a href="https://publications.waset.org/abstracts/14584/hepatitis-e-among-pregnant-women-in-urmia-iran" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14584.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">302</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">771</span> The Regulation on Human Exposure to Electromagnetic Fields for Brazilian Power System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hugo%20Manoel%20Olivera%20Da%20Silva">Hugo Manoel Olivera Da Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Ricardo%20Silva%20Th%C3%A9%20Pontes"> Ricardo Silva Thé Pontes</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this work, is presented an analysis of the Brazilian regulation on human exposure to electromagnetic fields, which provides limits to electric fields, magnetic and electromagnetic fields. The regulations for the electricity sector was in charge of the Agência Nacional de Energia Elétrica-ANEEL, the Brazilian Electricity Regulatory Agency, that made it through the Normative Resolution Nº 398/2010, resulting in a series of obligations for the agents of the electricity sector, especially in the areas of generation, transmission, and distribution. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adverse%20effects" title="adverse effects">adverse effects</a>, <a href="https://publications.waset.org/abstracts/search?q=electric%20energy" title=" electric energy"> electric energy</a>, <a href="https://publications.waset.org/abstracts/search?q=electric%20and%20magnetic%20fields" title=" electric and magnetic fields"> electric and magnetic fields</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20health" title=" human health"> human health</a>, <a href="https://publications.waset.org/abstracts/search?q=regulation" title=" regulation"> regulation</a> </p> <a href="https://publications.waset.org/abstracts/21066/the-regulation-on-human-exposure-to-electromagnetic-fields-for-brazilian-power-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21066.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">607</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">770</span> Automation of Kitchen Chemical in the Textile Industry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jos%C3%A9%20Luiz%20da%20Silva%20Neto">José Luiz da Silva Neto</a>, <a href="https://publications.waset.org/abstracts/search?q=Renato%20Sipelli%20Silva"> Renato Sipelli Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%89rick%20Arag%C3%A3o%20Ribeiro"> Érick Aragão Ribeiro</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The automation of industrial processes plays a vital role in industries today, becoming an integral and important part of the industrial process and modern production. The process control systems are designed to maximize production, reduce costs and minimize risks in production. However, these systems are generally not deployed methodologies and planning. So that this article describes the development of an automation system of a kitchen preparation of chemicals in the textile industry based on a retrofitting methodology that provides more quality into the process at a lower cost. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=automation" title="automation">automation</a>, <a href="https://publications.waset.org/abstracts/search?q=textile%20industry" title=" textile industry"> textile industry</a>, <a href="https://publications.waset.org/abstracts/search?q=kitchen%20chemical" title=" kitchen chemical"> kitchen chemical</a>, <a href="https://publications.waset.org/abstracts/search?q=information%20integration" title=" information integration"> information integration</a> </p> <a href="https://publications.waset.org/abstracts/41010/automation-of-kitchen-chemical-in-the-textile-industry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41010.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">427</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">769</span> Development and Evaluation of Novel Diagnostic Methods for Infectious Rhinotracheitis of Cattle</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wenxiao%20Liu">Wenxiao Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Kun%20Zhang"> Kun Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yongqing%20Li"> Yongqing Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bovine herpesvirus 1, a member of the genus Variellovirus of the subfamily Alphaherpesvirinae, has caused severe economic cost to the bovine industry. In this study, BoHV-1 glycerol protein gD was expressed in insect cells, and the purified gD was immunized in the Balb/C mice to generate monoclonal antibodies. Based on hybridoma cell fusion techniques, 20 monoclonal antibodies against Bovine herpesvirus 1 have been obtained. Further, mAb 3F8 with neutralizing activity and gD were applied to develop a blocking enzyme-linked immunosorbent assay (Elisa) for detecting neutralizing antibodies against BoHV-1, which shows a significant correlation between the blocking Elisa and VNT. The sensitivity and specificity of the test were estimated to be 94.59% and 93.42%, respectively. Furthermore, antibody pairing tests revealed that mAb 1B6 conjugated to fluorescence microspheres was used as the capture antibody, and mAb 3F9 was used as the detectable antibody to establish the immunochromatographic assay (ICS). The ICS was conducted to detect BoHV-1 in bovine samples with high sensitivity, specificity, and good stability. Clinical sample testing revealed that the results of ICS and real-time PCR have a coincidence rate of 95.42%. Our research confirmed that the ICS is a rapid and reliable method for the diagnosis of BoHV-1. In conclusion, our results lay a solid foundation for the prevention and control of BoHV-1 infection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bovine%20disease" title="bovine disease">bovine disease</a>, <a href="https://publications.waset.org/abstracts/search?q=BoHV-1" title=" BoHV-1"> BoHV-1</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=ICS%20assay" title=" ICS assay"> ICS assay</a> </p> <a href="https://publications.waset.org/abstracts/181179/development-and-evaluation-of-novel-diagnostic-methods-for-infectious-rhinotracheitis-of-cattle" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/181179.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">74</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">768</span> Observatory of Sustainability of the Algarve Region for Tourism: Proposal for Environmental and Sociocultural Indicators</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Miguel%20Jos%C3%A9%20Oliveira">Miguel José Oliveira</a>, <a href="https://publications.waset.org/abstracts/search?q=F%C3%A1tima%20Farinha"> Fátima Farinha</a>, <a href="https://publications.waset.org/abstracts/search?q=Elisa%20M.%20J.%20da%20Silva"> Elisa M. J. da Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Rui%20Lan%C3%A7a"> Rui Lança</a>, <a href="https://publications.waset.org/abstracts/search?q=Manuel%20Duarte%20Pinheiro"> Manuel Duarte Pinheiro</a>, <a href="https://publications.waset.org/abstracts/search?q=C%C3%A1tia%20Miguel"> Cátia Miguel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Observatory of Sustainability of the Algarve Region for Tourism (OBSERVE) will be a valuable tool to assess the sustainability of this region. The OBSERVE tool is designed to provide data and maintain an up-to-date, consistent set of indicators defined to describe the region on the environmental, sociocultural, economic and institutional domains. This ongoing two-year project has the active participation of the Algarve’s stakeholders, since they were consulted and asked to participate in the discussion for the indicators proposal. The environmental and sociocultural indicators chosen must indicate the characteristics of the region and should be in alignment with other global systems used to monitor the sustainability. This paper presents a review of sustainability indicators systems that support the first proposal for the environmental and sociocultural indicators. Others constraints are discussed, namely the existing data and the data available in digital platforms in a format suitable for automatic importation to the platform of OBSERVE. It is intended that OBSERVE will be a valuable tool to assess the sustainability of the region of Algarve. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Algarve" title="Algarve">Algarve</a>, <a href="https://publications.waset.org/abstracts/search?q=development" title=" development"> development</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20indicators" title=" environmental indicators"> environmental indicators</a>, <a href="https://publications.waset.org/abstracts/search?q=observatory" title=" observatory"> observatory</a>, <a href="https://publications.waset.org/abstracts/search?q=sociocultural%20indicators" title=" sociocultural indicators"> sociocultural indicators</a>, <a href="https://publications.waset.org/abstracts/search?q=sustainability" title=" sustainability"> sustainability</a>, <a href="https://publications.waset.org/abstracts/search?q=tourism" title=" tourism"> tourism</a> </p> <a href="https://publications.waset.org/abstracts/107791/observatory-of-sustainability-of-the-algarve-region-for-tourism-proposal-for-environmental-and-sociocultural-indicators" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/107791.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">175</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">767</span> Evaluation of the Hepatitis C Virus and Classical and Modern Immunoassays Used Nowadays to Diagnose It in Tirana</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Stela%20Papa">Stela Papa</a>, <a href="https://publications.waset.org/abstracts/search?q=Klementina%20Puto"> Klementina Puto</a>, <a href="https://publications.waset.org/abstracts/search?q=Migena%20Pllaha"> Migena Pllaha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> HCV is a hepatotropic RNA virus, transmitted primarily via the blood route, which causes progressive disease such as chronic hepatitis, liver cirrhosis, or hepatocellular carcinoma. HCV nowadays is a global healthcare problem. A variety of immunoassays including old and new technologies are being applied to detect HCV in our country. These methods include Immunochromatography assays (ICA), Fluorescence immunoassay (FIA), Enzyme linked fluorescent assay (ELFA), and Enzyme linked immunosorbent assay (ELISA) to detect HCV antibodies in blood serum, which lately is being slowly replaced by more sensitive methods such as rapid automated analyzer chemiluminescence immunoassay (CLIA). The aim of this study is to estimate HCV infection in carriers and chronic acute patients and to evaluate the use of new diagnostic methods. This study was realized from September 2016 to May 2018. During this study period, 2913 patients were analyzed for the presence of HCV by taking samples from their blood serum. The immunoassays performed were ICA, FIA, ELFA, ELISA, and CLIA assays. Concluding, 82% of patients taken in this study, resulted infected with HCV. Diagnostic methods in clinical laboratories are crucial in the early stages of infection, in the management of chronic hepatitis and in the treatment of patients during their disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CLIA" title="CLIA">CLIA</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=Hepatitis%20C%20virus" title=" Hepatitis C virus"> Hepatitis C virus</a>, <a href="https://publications.waset.org/abstracts/search?q=immunoassay" title=" immunoassay"> immunoassay</a> </p> <a href="https://publications.waset.org/abstracts/110783/evaluation-of-the-hepatitis-c-virus-and-classical-and-modern-immunoassays-used-nowadays-to-diagnose-it-in-tirana" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/110783.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">153</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">766</span> Anti-TNF: Possibilities of Rising Anti-Phosphorylcholine Antibodies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Md.%20Mizanur%20Rahman">Md. Mizanur Rahman</a>, <a href="https://publications.waset.org/abstracts/search?q=Anquan%20Liu"> Anquan Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Anna%20Frosteg%C3%A5rd"> Anna Frostegård</a>, <a href="https://publications.waset.org/abstracts/search?q=Johan%20Frosteg%C3%A5rd"> Johan Frostegård</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The role of the human immune system is essential in cardiovascular diseases and atherosclerosis. Activated cells in atherosclerosis produce abundant amounts of cytokines, but the exact mechanisms involved in the effects of these inflammatory cytokines are not clear in atherosclerosis. In a large clinical cohort, we have previously determined that antibodies against phosphorylcholine (anti-PC) are negatively and independently associated with both development of atherosclerosis and also a low risk of cardiovascular disease. Further, we reported that rheumatoid arthritis patients who were non-responders to TNF-inhibitors, where those with low anti-PC levels. Upon anti-TNF treatment, anti-PC levels increased. We, therefore, hypothesised that proinflammatory cytokines such as TNF could play a role in anti-PC regulation. Peripheral blood mononuclear cells (PBMC) were cultured with or without TNF and anti-TNF. The cell supernatants were collected after six days for ELISA measurements. In separate experiments, cells were cultured for 24 hours in both polystyrene plates and ELISPOT plates under a similar condition for ELISA and ELISPOT assays respectively. Total RNA was extracted after 6 hours of cell culture to perform RT-qPCR. Cell viability was confirmed by trypan blue staining and MTT assays. ELISA measurements detected less than 40% of anti-PC in TNF-treated cells, in comparison to control cells, whereas anti-PC production was recovered by anti-TNF treatment. ELISPOT assays showed that TNF suppresses anti-PC production by inhibiting anti-PC producing B-cells. In addition, RT-qPCR and ELISA showed that TNF also has effects also on B-cell activation as BAFF expression was inhibited by TNF treatment. Atherosclerosis is a major cause of cardiovascular diseases, but anti-PC is a protection marker for atherosclerosis development. Our findings show that TNF is a negative regulator of anti-PC production. Immune modulation and rising of anti-PC could be of major significance for the patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti-PC" title="anti-PC">anti-PC</a>, <a href="https://publications.waset.org/abstracts/search?q=Anti-TNF" title=" Anti-TNF"> Anti-TNF</a>, <a href="https://publications.waset.org/abstracts/search?q=atherosclerosis" title=" atherosclerosis"> atherosclerosis</a>, <a href="https://publications.waset.org/abstracts/search?q=cardiovascular%20diseases" title=" cardiovascular diseases"> cardiovascular diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphorylecholine" title=" phosphorylecholine"> phosphorylecholine</a> </p> <a href="https://publications.waset.org/abstracts/44750/anti-tnf-possibilities-of-rising-anti-phosphorylcholine-antibodies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44750.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">243</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">765</span> The Cytomegalovirus Infection among Iranian Kidney Graft Recipients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakieh%20Rostamzadeh">Zakieh Rostamzadeh </a>, <a href="https://publications.waset.org/abstracts/search?q=Nariman%20Sepehrvand-Zahra%20Shirmohamadi"> Nariman Sepehrvand-Zahra Shirmohamadi </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Cytomegalovirus (CMV) infection is one of the most common infectious problems following kidney transplantation. In this study, we are aimed to investigate the CMV infection in the setting of renal transplant recipients in Urmia-Iran, using both ELISA and polymerase chain reaction (PCR) methods. Methods: Ninety-six renal transplant recipients were selected randomly and enrolled in a cross-sectional study. Blood sampling was done via venipuncture, and all sera were investigated for anti-CMV IgM, and the seropositive cases in association with 14 randomly selected seronegative cases were investigated with PCR assay. Results: Thirty-three patients (34.3%) were seropositive for anti-CMV IgM, 3 patients (3.1%) were in borderline range, and 60 patients (62.5%) were seronegative. By considering the patients with borderline anti-CMV IgM levels as seropositive, 37.5% were seropositive for anti-CMV IgM. Among 36 seropositive cases, the CMV infection was confirmed in 19 (52.7%) of them using PCR. Age (P = 0.40), educational status (P = 0.77), history of pre-transplantation dialysis (0.52), history of blood transfusion (P = 0.52), and immunosuppressive regimen were not statistically different among recipients with positive versus negative CMV PCR study results. Conclusion: The seroprevalence of CMV infection was demonstrated to be high in renal transplant recipients of Urmia-Iran. The rate was higher compared to several previous reports in the literature. ELISA method has an appropriate sensitivity to screen the recipients for CMV infection but considering its relatively low specificity, the seropositive cases are better to be confirmed by further PCR study. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytomegalovirus" title="cytomegalovirus">cytomegalovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=renal%20transplantation" title=" renal transplantation"> renal transplantation</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=IgM" title=" IgM"> IgM</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a> </p> <a href="https://publications.waset.org/abstracts/14587/the-cytomegalovirus-infection-among-iranian-kidney-graft-recipients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14587.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">303</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">764</span> Seroprevalence Study of Cystic Echinococcosis and Its Associated Risk Factors in Fars Province, Southern Iran</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20Reza%20Tahamtan">Mahmoud Reza Tahamtan</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Saleh%20Bahreini"> Mohammad Saleh Bahreini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and Purpose: Cystic echinococcosis, caused by the larval stage of Echinococcus granulosus, is a common parasitic infection of humans and is endemic in many parts of the world, including Iran. So that, one percent of those admitted to surgery departments are hydatid cyst patients, and using the ELISA method, the infection rate has been reported in different regions of Iran from 1.2% to 21.4%. Therefore, the aim of this study was to investigate the seroepidemiology of human hydatid cysts in Fars province, southern Iran, by ELISA method. Methods: In this cross-sectional study, 600 serum samples of persons who were referred to the laboratory of Nemazi Hospital in Shiraz for normal tests were examined for the presence of specific Anti-IgG antibodies to hydatid cysts by ELISA method. During the sampling, a structured questionnaire was used to obtain social data of individuals with determinants of risk factors for Cystic echinococcosis. Finally, the results of the ELISA test, along with demographic information completed by individuals, were analyzed using SPSS software. Results: The average age of the subjects in this study was 40.01 ± 9.166. The prevalence of hydatidosis was reported as 5.66% (34/600). The disease was more in the age group of 21-30, people living in villages, working in rural areas, and people with a history of other parasitic diseases. Statistically, a significant difference was observed between the prevalence of the disease and two risk factors, contact with dogs (OR= 0.042; 95%CI: 0.014-0.12; P= 0.001) and washing vegetables with water (OR= 0.08; 95%CI: 0.011-0.56; P= 0.012). Conclusion: The present study showed that hydatid cyst disease has a significant prevalence in this area. Also, based on the results, contact with dogs and not properly washing vegetables are two important factors of disease transmission. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Echinococcus%20granulosus" title="Echinococcus granulosus">Echinococcus granulosus</a>, <a href="https://publications.waset.org/abstracts/search?q=Cystic%20echinococcosis" title=" Cystic echinococcosis"> Cystic echinococcosis</a>, <a href="https://publications.waset.org/abstracts/search?q=hydatid%20cyst" title=" hydatid cyst"> hydatid cyst</a>, <a href="https://publications.waset.org/abstracts/search?q=Fars%20province" title=" Fars province"> Fars province</a> </p> <a href="https://publications.waset.org/abstracts/172678/seroprevalence-study-of-cystic-echinococcosis-and-its-associated-risk-factors-in-fars-province-southern-iran" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/172678.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">97</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">763</span> Effect of Ultrasound on the Hydrolysis of Soy Oil Catalyzed by 1,3-Specific Lipase Abstract </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jamal%20Abd%20Awadallak">Jamal Abd Awadallak</a>, <a href="https://publications.waset.org/abstracts/search?q=Thiago%20Olinek%20Reinehr"> Thiago Olinek Reinehr</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduardo%20Raizer"> Eduardo Raizer</a>, <a href="https://publications.waset.org/abstracts/search?q=Deise%20Molinari"> Deise Molinari</a>, <a href="https://publications.waset.org/abstracts/search?q=Edson%20Antonio"> Edson Antonio</a>, <a href="https://publications.waset.org/abstracts/search?q=Camila%20da%20Silva%20da%20Silva"> Camila da Silva da Silva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The hydrolysis of soy oil catalyzed by 1,3-specific enzyme (Lecitase Ultra) in a well-stirred bioreactor was studied. Two forms of applications of the ultrasound were evaluated aiming to increase reaction rates, wherein the use of probe ultrasound associated with the use of surfactant to pre-emulsify the substrate showed the best results. Two different reaction periods were found: the first where the ultrasound has great influence on reaction rates, and the second where ultrasound influence is minimal. Studies on the time of pre-emulsification, surfactant concentration and enzyme concentration showed that the initial rate of hydrolysis depends on the interfacial area between the oil phase and the aqueous phase containing the enzyme. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=specific%20enzyme" title="specific enzyme">specific enzyme</a>, <a href="https://publications.waset.org/abstracts/search?q=free%20fatty%20acids" title=" free fatty acids"> free fatty acids</a>, <a href="https://publications.waset.org/abstracts/search?q=Hydrolysis" title=" Hydrolysis"> Hydrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=lecitase%20ultra" title=" lecitase ultra"> lecitase ultra</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound" title=" ultrasound"> ultrasound</a> </p> <a href="https://publications.waset.org/abstracts/20337/effect-of-ultrasound-on-the-hydrolysis-of-soy-oil-catalyzed-by-13-specific-lipase-abstract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20337.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">578</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">762</span> Investigation of Enterotoxigenic Staphylococcus aureus in Kitchen of Catering</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=%C3%87i%C4%9Fdem%20Sezer">Çiğdem Sezer</a>, <a href="https://publications.waset.org/abstracts/search?q=Aksem%20Aksoy"> Aksem Aksoy</a>, <a href="https://publications.waset.org/abstracts/search?q=Leyla%20Vatansever"> Leyla Vatansever</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study has been done for the purpose of evaluation of public health and identifying of enterotoxigenic Staphyloccocus aureus in kitchen of catering. In the kitchen of catering, samples have been taken by swabs from surface of equipments which are in the salad section, meat section and bakery section. Samples have been investigated with classical cultural methods in terms of Staphyloccocus aureus. Therefore, as a 10x10 cm area was identified (salad, cutting and chopping surfaces, knives, meat grinder, meat chopping surface) samples have been taken with sterile swabs with helping FTS from this area. In total, 50 samples were obtained. In aseptic conditions, Baird-Parker agar (with egg yolk tellurite) surface was seeded with swabs. After 24-48 hours of incubation at 37°C, the black colonies with 1-1.5 mm diameter and which are surrounded by a zone indicating lecithinase activity were identified as S. aureus after applying Gram staining, catalase, coagulase, glucose and mannitol fermentation and termonuclease tests. Genotypic characterization (Staphylococcus genus and S.aureus species spesific) of isolates was performed by PCR. The ELISA test was applied to the isolates for the identification of staphylococcal enterotoxins (SET) A, B, C, D, E in bacterial cultures. Measurements were taken at 450 nm in an ELISA reader using an Ridascreen-Total set ELISA test kit (r-biopharm R4105-Enterotoxin A, B, C, D, E). The results were calculated according to the manufacturer’s instructions. A total of 50 samples of 97 S. aureus was isolated. This number has been identified as 60 with PCR analysis. According to ELISA test, only 1 of 60 isolates were found to be enterotoxigenic. Enterotoxigenic strains were identified from the surface of salad chopping and cutting. In the kitchen of catering, S. aureus identification indicates a significant source of contamination. Especially, in raw consumed salad preparation phase of contamination is very important. This food can be a potential source of food-borne poisoning their terms, and they pose a significant risk to consumers have been identified. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Staphylococcus%20aureus" title="Staphylococcus aureus">Staphylococcus aureus</a>, <a href="https://publications.waset.org/abstracts/search?q=enterotoxin" title=" enterotoxin"> enterotoxin</a>, <a href="https://publications.waset.org/abstracts/search?q=catering" title=" catering"> catering</a>, <a href="https://publications.waset.org/abstracts/search?q=kitchen" title=" kitchen"> kitchen</a>, <a href="https://publications.waset.org/abstracts/search?q=health" title=" health"> health</a> </p> <a href="https://publications.waset.org/abstracts/15962/investigation-of-enterotoxigenic-staphylococcus-aureus-in-kitchen-of-catering" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15962.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">402</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">761</span> Energy Efficient Heterogeneous System for Wireless Sensor Networks (WSN)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jos%C3%A9%20Anderson%20Rodrigues%20de%20Souza">José Anderson Rodrigues de Souza</a>, <a href="https://publications.waset.org/abstracts/search?q=Teles%20de%20Sales%20Bezerra"> Teles de Sales Bezerra</a>, <a href="https://publications.waset.org/abstracts/search?q=Saulo%20Aislan%20da%20Silva%20Eleuterio"> Saulo Aislan da Silva Eleuterio</a>, <a href="https://publications.waset.org/abstracts/search?q=Jeronimo%20Silva%20Rocha">Jeronimo Silva Rocha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mobile devices are increasingly occupying sectors of society and one of its most important features is mobility. However, the use of mobile devices is subject to the lifetime of the batteries. Thus, the use of energy batteries has become an important issue in the study of wireless network technologies. In this context, new solutions that enable aggregate energy efficiency not only through energy saving, and principally they are evaluated from a more realistic model of energy discharge, if easy adaptation to existing protocols. This paper presents a study on the energy needed and the lifetime for Wireless Sensor Networks (WSN) using a heterogeneous network and applying the LEACH protocol. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=wireless%20sensor%20networks" title="wireless sensor networks">wireless sensor networks</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20efficiency" title=" energy efficiency"> energy efficiency</a>, <a href="https://publications.waset.org/abstracts/search?q=heterogeneous" title=" heterogeneous"> heterogeneous</a>, <a href="https://publications.waset.org/abstracts/search?q=LEACH%20protocol" title=" LEACH protocol"> LEACH protocol</a> </p> <a href="https://publications.waset.org/abstracts/19737/energy-efficient-heterogeneous-system-for-wireless-sensor-networks-wsn" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19737.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">580</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">760</span> Preliminary Prospecting on the Distribution of the Disease of Citrus Tristeza Orchards in the Province of Chlef</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20Djelloul%20Berkane">Ibrahim Djelloul Berkane</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A survey was conducted to assess the presence of the virus in Citrus tristeza one of the main citrus regions of Algeria, namely the Chlef region, using the technique of Direct Tissue Print Immunoprinting Assay (DTBIA) and the Double Sandwich ELISA antibodies. A nursery citrus, lumber yards, and commercial orchards, which are the main varieties cultivated citrus were subjected to samples collected samples for laboratory analysis. 0.91% of the plants tested orchards were infected with CTV, while no positive case was detected at the nursery the yard, however, it is reported that an alarming rate of 10,5% of orchards tested at the common Chettia were infected with tristeza virus. The investigation was launched to identify the vector species tristeza revealed the presence of a vector is important Aphis gossypii. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aphis" title="aphis">aphis</a>, <a href="https://publications.waset.org/abstracts/search?q=chlef" title=" chlef"> chlef</a>, <a href="https://publications.waset.org/abstracts/search?q=citrus" title=" citrus"> citrus</a>, <a href="https://publications.waset.org/abstracts/search?q=DAS-ELISA" title=" DAS-ELISA"> DAS-ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=DTBIA" title=" DTBIA"> DTBIA</a>, <a href="https://publications.waset.org/abstracts/search?q=tristeza" title=" tristeza"> tristeza</a> </p> <a href="https://publications.waset.org/abstracts/56707/preliminary-prospecting-on-the-distribution-of-the-disease-of-citrus-tristeza-orchards-in-the-province-of-chlef" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56707.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">303</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">759</span> ELISA Based hTSH Assessment Using Two Sensitive and Specific Anti-hTSH Polyclonal Antibodies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maysam%20Mard-Soltani">Maysam Mard-Soltani</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamad%20Javad%20Rasaee"> Mohamad Javad Rasaee</a>, <a href="https://publications.waset.org/abstracts/search?q=Saeed%20Khalili"> Saeed Khalili</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdol%20Karim%20Sheikhi"> Abdol Karim Sheikhi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehdi%20Hedayati"> Mehdi Hedayati</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Production of specific antibody responses against hTSH is a cumbersome process due to the high identity between the hTSH and the other members of the glycoprotein hormone family (FSH, LH and HCG) and the high identity between the human hTSH and host animals for antibody production. Therefore, two polyclonal antibodies were purified against two recombinant proteins. Four possible ELISA tests were designed based on these antibodies. These ELISA tests were checked against hTSH and other glycoprotein hormones, and their sensitivity and specificity were assessed. Bioinformatics tools were used to analyze the immunological properties. After the immunogen region selection from hTSH protein, c terminal of B hTSH was selected and applied. Two recombinant genes, with these cut pieces (first: two repeats of C terminal of B hTSH, second: tetanous toxin+B hTSH C terminal), were designed and sub-cloned into the pET32a expression vector. Standard methods were used for protein expression, purification, and verification. Thereafter, immunizations of the white New Zealand rabbits were performed and the serums of them were used for antibody titration, purification and characterization. Then, four ELISA tests based on two antibodies were employed to assess the hTSH and other glycoprotein hormones. The results of these assessments were compared with standard amounts. The obtained results indicated that the desired antigens were successfully designed, sub-cloned, expressed, confirmed and used for <em>in vivo</em> immunization. The raised antibodies were capable of specific and sensitive hTSH detection, while the cross reactivity with the other members of the glycoprotein hormone family was minimum. Among the four designed tests, the test in which the antibody against first protein was used as capture antibody, and the antibody against second protein was used as detector antibody did not show any hook effect up to 50 miu/l. Both proteins have the ability to induce highly sensitive and specific antibody responses against the hTSH. One of the antibody combinations of these antibodies has the highest sensitivity and specificity in hTSH detection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hTSH" title="hTSH">hTSH</a>, <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title=" bioinformatics"> bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20expression" title=" protein expression"> protein expression</a>, <a href="https://publications.waset.org/abstracts/search?q=cross%20reactivity" title=" cross reactivity"> cross reactivity</a> </p> <a href="https://publications.waset.org/abstracts/84047/elisa-based-htsh-assessment-using-two-sensitive-and-specific-anti-htsh-polyclonal-antibodies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84047.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">189</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">758</span> Characterization of Monoclonal Antibodies Specific for Synthetic Cannabinoids</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hiroshi%20Nakayama">Hiroshi Nakayama</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuji%20Ito"> Yuji Ito</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Synthetic cannabinoids have attracted much public attention recently in Japan. 1-pentyl-3-(1-naphthoyl)-indole (JWH-018), 1-pentyl-2-methyl-3-(1-naphthoyl) indole (JWH-015), 1-(5-fluoropentyl)-3- (1-(2,2,3,3- tetramethylcyclopropyl)) indole (XLR-11) and 1-methyl-3- (1-admantyl) indole (JWH-018 adamantyl analog) are known as synthetic cannabinoids and are also considered dangerous illegal drugs in Japan. It has become necessary to develop sensitive and useful methods for detection of synthetic cannabinoids. We produced two monoclonal antibodies (MAb) against synthetic cannabinoids, named NT1 (IgG1) and NT2 (IgG1), using Hybridoma technology. The cross-reactivity of these produced MAbs was evaluated using a competitive enzyme-linked immunosorbent assay (ELISA). In the results, we found both of these antibodies recognize many kinds of synthetic cannabinoids analog. However, neither of these antibodies recognizes naphtoic acid, 1-methyl-indole and indole known as a raw material of synthetic cannabinoid. Thus, the MAbs produced in this study could be a useful tool for the detection of synthetic cannabinoids. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ELISA" title="ELISA">ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=monoclonal%20antibody" title=" monoclonal antibody"> monoclonal antibody</a>, <a href="https://publications.waset.org/abstracts/search?q=sensor" title=" sensor"> sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=synthetic%20cannabinoid" title=" synthetic cannabinoid"> synthetic cannabinoid</a> </p> <a href="https://publications.waset.org/abstracts/51072/characterization-of-monoclonal-antibodies-specific-for-synthetic-cannabinoids" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/51072.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">355</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Elisa%20Silva&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Elisa%20Silva&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Elisa%20Silva&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Elisa%20Silva&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Elisa%20Silva&page=6">6</a></li> <li class="page-item"><a class="page-link" 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