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aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">Why does media matter?</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-2704 post type-post status-publish format-standard has-post-thumbnail category-bioprocessing tag-car-t-cell-therapy-manufacturing tag-cell-therapy-media tag-ex-vivo-cell-culture tag-gibco-cts-optmizer-one-sfm tag-serum-free-t-cell-medium tag-t-cell-culture-media tag-t-cell-culture-medium tag-t-cell-expansion-medium tag-t-cell-manufacturing tag-t-cell-media entry" aria-label="Why does media matter?"><header class="entry-header"><h1 class="entry-title">Why does media matter?</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/lifeinthelabstaff/" title="Posts by Life in the Lab Staff" rel="author">Life in the Lab Staff</a> </span> <span class="post-info-date"> 03.11.2024 </span> </header><div class="entry-content"><p><img decoding="async" class="alignnone wp-image-2706 size-full" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cts-optmizer-one-sfm.png" alt="CTS OpTmizer One SFM family of products" width="1570" height="1024" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cts-optmizer-one-sfm.png 1570w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cts-optmizer-one-sfm-300x196.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cts-optmizer-one-sfm-1024x668.png 1024w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cts-optmizer-one-sfm-768x501.png 768w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cts-optmizer-one-sfm-1536x1002.png 1536w" sizes="(max-width: 1570px) 100vw, 1570px" /></p> <p>Every material used at every stage of a cell therapy workflow can impact the efficacy of the final deliverable drug. <a href="https://www.thermofisher.com/in/en/home/life-science/bioproduction/gibco-bioprocessing/cell-therapy.html">Gibco CTS OpTmizer One Serum Free Medium (SFM)</a> takes this into account. Not only is this thoughtfully designed nutrition for T-cells, but it is an animal origin-free (AOF) formulation.</p> <p>Why is that important? The risk of contamination with an adventitious agent is a serious concern when starting with a small sample of cells and a waiting patient. An animal origin-free formulation helps mitigate this risk.</p> <p>Of course, there are other critical factors that a cell culture medium directly influences. Manufacturers tend to prefer the early memory T-cell phenotype. They also need high viability, low variability, and a medium that is flexible to scale up to other vessels.</p> <h2>Enhanced T-cell expansion, high viability, and early memory cell phenotype</h2> <p> </p> <p>The Gibco CTS OpTmizer family of media has been used in over 200 clinical trials, so it’s fair to say we know media. Data shows that our newest addition—the CTS OpTmizer One SFM—helps to meet manufacturer needs with robust T-cell expansion, while maintaining high T-cell viability and early memory phenotype.</p> <p><a href="https://assets.thermofisher.com/TFS-Assets/BPD/Application-Notes/cts-optmizer-one-sfm-offers-enhanced-t-cell-expansion-high-viability-application-note.pdf" target="_blank" rel="noopener">In a study</a> of healthy donors, the CTS OpTmizer One SFM supported comparable or higher total cell counts relative to those supported with two AOF media and one xeno-free medium from other suppliers (<strong>Figure 1</strong>).</p> <p> </p> <p><img decoding="async" class="aligncenter wp-image-2707 size-full" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion.png" alt="CTS OpTmizer One SFM supported comparable or statistically higher average total cell counts" width="3460" height="1865" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion.png 3460w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion-300x162.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion-1024x552.png 1024w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion-768x414.png 768w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion-1536x828.png 1536w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/healthy-donor-cell-expansion-2048x1104.png 2048w" sizes="(max-width: 3460px) 100vw, 3460px" /></p> <p style="text-align: center"><strong>Figure 1. Healthy donor cell expansion.</strong></p> <p>In addition, through day 10, CD3+ cells with CTS OpTmizer One SFM maintained comparable or higher average expression of CCR7+ CD62L+, CD27+ CD62L+, and CD45RO+ CD62L+ early memory cell phenotype markers (<strong>Figure 2</strong>).</p> <p><a href="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype.png"><img loading="lazy" decoding="async" class="wp-image-2710 alignnone" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-300x162.png" alt=" By day 10, with CTS OpTmizer One SFM, CD3+ cells demonstrated a comparable, or statistically higher average expression of early memory cell phenotype markers" width="351" height="190" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-300x162.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-1024x552.png 1024w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-768x414.png 768w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-1536x828.png 1536w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-2048x1104.png 2048w" sizes="(max-width: 351px) 100vw, 351px" /></a> <a href="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2.png"><img loading="lazy" decoding="async" class="wp-image-2711 alignnone" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2-300x162.png" alt="CTS OpTmizer One SFM supported comparable or statistically higher average total cell counts" width="351" height="190" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2-300x162.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2-1024x552.png 1024w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2-768x414.png 768w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2-1536x828.png 1536w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cel-phenotype-image-2-2048x1104.png 2048w" sizes="(max-width: 351px) 100vw, 351px" /></a><a href="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3.png"><img loading="lazy" decoding="async" class="wp-image-2712 aligncenter" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3-300x177.png" alt="CTS OpTmizer One SFM supported comparable or statistically higher average total cell counts" width="351" height="207" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3-300x177.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3-1024x603.png 1024w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3-768x452.png 768w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3-1536x904.png 1536w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/t-cell-phenotype-img-3-2048x1206.png 2048w" sizes="(max-width: 351px) 100vw, 351px" /></a></p> <p style="text-align: center"><strong>Figure 2. Healthy Donor T cell Phenotype.</strong></p> <p>In a study of diseased donors, CTS OpTmizer One SFM supported a more consistent and stronger expansion of 284-fold by day 10 relative to media from other suppliers for two donors with AML (<strong>Figure 3</strong>). While cells from two CLL diseased donors demonstrated a higher average expansion of 80-fold with CTS OpTmizer SFM.</p> <p><a href="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/aml-donor-cells.png"><img loading="lazy" decoding="async" class="alignnone size-medium wp-image-2718" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/aml-donor-cells-300x162.png" alt="By day 10, AML donor cells achieved more consistent and robust 284-fold expansion with CTS OpTmizer One SFM compared to those with the CM1 and CM3 media from other suppliers" width="340" height="188" /></a> <a href="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cll-donor-cells.png"><img loading="lazy" decoding="async" class="alignnone size-medium wp-image-2719" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/03/cll-donor-cells-300x162.png" alt="by day 10 showed a higher near 80-fold average expansion with CTS OpTmizer One SFM compared to those with the CM1 medium" width="340" height="188" /></a></p> <p style="text-align: center"><strong>Figure 3. Diseased donor cell expansion.</strong></p> <p>The media also supported comparably high viability and more consistent maintenance of early memory cell phenotype than the other suppliers’ media in both diseased donor cells.</p> <p>To ask again, why is this important? The answer is that cell therapy media plays a crucial role in the potency and efficacy of the final drug product. That is why thoughtfully designed nutrition is so important.</p> <p>Read about these experiments in detail in this application note, discussing how <a href="https://assets.thermofisher.com/TFS-Assets/BPD/Application-Notes/cts-optmizer-one-sfm-offers-enhanced-t-cell-expansion-high-viability-application-note.pdf" target="_blank" rel="noopener">CTS OpTmizer One SFM offers enhanced T-cell expansion and high viability while maintaining early memory cell phenotype.</a></p> <p>Please visit our website to learn more about <a href="https://www.thermofisher.com/us/en/home/life-science/bioproduction/gibco-bioprocessing/cell-therapy/media.html">cell therapy media</a>.</p> <h2>Recommended resources</h2> <ul> <li><strong>Application note: </strong><a href="https://assets.thermofisher.com/TFS-Assets/BPD/Application-Notes/cts-optmizer-one-sfm-offers-enhanced-t-cell-expansion-high-viability-application-note.pdf" target="_blank" rel="noopener">CTS OpTmizer One SFM offers enhanced T cell expansion and high viability while maintaining early memory cell phenotype</a></li> <li><strong>Flyer:</strong> <a href="https://assets.thermofisher.com/TFS-Assets/BPD/Flyers/cell-nutrition-delivers-young-healthy-tcells-flyer.pdf" target="_blank" rel="noopener">Cell nutrition that delivers young, healthy T cells for your process</a></li> <li><strong>Whitepaper: </strong><a href="https://assets.thermofisher.com/TFS-Assets/BPD/Reference-Materials/gibco-bpc-line-expansion-whitepaper.pdf" target="_blank" rel="noopener">Streamlining your cell therapy workflow: exploring the benefits of larger bioprocess container sizes</a></li> </ul> <p class="react-spectrum-RichTextEditor-paragraph"> Intended use of the products mentioned on this page vary. 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