Register: On-Demand Mouse Hematopoietic Stem and Progenitor Cells CFU Assay Course

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Dr. Damen has extensive experience in the field of hematopoiesis, where her key strengths lie in stem cell biology, protein biochemistry, and cell signaling, especially in the area of erythropoietin. In her role as Senior Scientific Advisor, Hematopoiesis, as well as her past experience as Director of Contract Assay Services at STEMCELL, Dr. Damen has conducted hundreds of customized studies involving the CFU assay to provide pharmaceutical and biotechnology clients with solutions to drug development questions. Dr. Damen actively participates in educational initiatives, regularly providing online talks and presentations at high-profile conferences, such as the Society of Toxicology Annual Meeting and the International Society of Cellular Therapy (ISCT). At STEMCELL, Dr. Damen has been involved in customer training in hematopoietic stem cell biology and colony identification since 2002. </p> </div> <div class="clear"></div> </div> </div> </div> <div class="faq"> <h1 class="question"><i class="fa fa-plus-circle"></i> <span>Dr. Tina Jeng, Scientist, Scientific Support, Hematology</span></h1> <div class="answer" style="display: none;"> <div class="support-info"> <div class="speaker-photo grid_2_lg grid_2_md grid_3_sm grid_3_xs"> <img alt="Dr. Tina Jeng" src="https://cdn.stemcell.com/media/images/pages/training/tina-jeng-350x350.jpg" style="border: 1px solid #F0F0EB;"> </div> <div class="grid_10_lg grid_10_md grid_9_sm grid_12_xs right omega"> <p> Tina Jeng is a Scientist specializing in the hematology portfolio in the Education & Product and Scientific Support (PSS) department at STEMCELL Technologies. She obtained her BSc in Molecular Biology and Biochemistry from the University of British Columbia, where she garnered technical skills and hands-on experience in various in vitro and in vivo hematopoietic stem and progenitor cell (HSPC) characterization assays. Subsequently, she joined Contract Assay Services at STEMCELL Technologies, where she was responsible for designing and executing customized projects to evaluate the effects of candidate therapeutics, including small molecule compounds and biologics, for more than 150 BioTech and Pharmaceutical customers across the globe. In her current role as the Hematology Scientist at STEMCELL, she provides product and scientific support to customers, distributors, and staff globally. She also contributes to various internal and external training programs for products, protocols, and workflows in the hematology portfolio. </p> </div> <div class="clear"></div> </div> </div> </div> </div> <div class="clear"></div> </div> <div class="support-info section" id="curriculum"> <h3>Course Curriculum</h3> <div class="grid_12_lg grid_12_md support-sect"> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="1"> <div class="th">Session 1: Introduction to Mouse Hematopoiesis</div> </div> </div> <div class="tbody"> <div class="tr" row-num="2"> <div class="td top" valign="top"> <p>Critical characteristics of hematopoietic stem vs. progenitor cells and the basic biology of mouse hematopoiesis.</p> <p><strong>Mini Quiz:</strong> Test Your Knowledge</p> <br> <p><em>Estimated time for completion: 20 minutes</em></p> </div> </div> </div> </div> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="3"> <div class="th">Session 2: Measuring Mouse Hematopoiesis</div> </div> </div> <div class="tbody"> <div class="tr" row-num="4"> <div class="td top" valign="top"> <ul class="disc"> <li><strong>Part 1:</strong> Phenotypic assays</li> <li><strong>Part 2:</strong> Functional assays</li> </ul> <p><strong>Mini Quiz:</strong> Test Your Knowledge</p> <br> <p><em>Estimated time for completion: 30 minutes</em></p> </div> </div> </div> </div> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="5"> <div class="th">Session 3: Mouse CFU Assay Technical Considerations</div> </div> </div> <div class="tbody"> <div class="tr" row-num="6"> <div class="td top" valign="top"> <ul class="disc"> <li><strong>Part 1:</strong> Choosing a source of mouse HSPCs</li> <li><strong>Part 2:</strong> Sample collection and preparation</li> <li><strong>Part 3:</strong> Choosing a MethoCult™ formulation</li> <li><strong>Part 4:</strong> CFU assay protocol and analysis overview</li> <li><strong>Part 5:</strong> Standardization of the CFU assay</li> </ul> <p><strong>Mini Quiz:</strong> Test Your Knowledge</p> <p><strong>Virtual Lab:</strong> Step-by-step narrated “<a href="https://www.stemcell.com/technical-resources/cfu-assay-instructions-using-smartdish-for-custom-hematopoietic-training-courses.html">How to Set Up the CFU Assay for Custom Hematopoietic Training Courses Using SmartDish™</a>”</p> <br> <p><em>Estimated time for completion: 60 minutes</em></p> </div> </div> </div> </div> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="7"> <div class="th">Session 4: Designing Your Mouse CFU Assay</div> </div> </div> <div class="tbody"> <div class="tr" row-num="8"> <div class="td top" valign="top"> <ul class="disc"> <li><strong>Part 1:</strong> Customizing the CFU assay for your application</li> <li><strong>Part 2:</strong> Optimizing the CFU assay protocol</li> <li><strong>Part 3:</strong> Case study</li> </ul> <p><strong>Mini Quiz:</strong> Test Your Knowledge</p> <br> <p><em>Estimated time for completion: 35 minutes</em></p> </div> </div> </div> </div> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="7"> <div class="th">Session 5: Manual Mouse Colony Classification and Enumeration</div> </div> </div> <div class="tbody"> <div class="tr" row-num="8"> <div class="td top" valign="top"> <ul class="disc"> <li><strong>Part 1:</strong> Customizing the CFU assay for your application</li> <li><strong>Part 2:</strong> Optimizing the CFU assay protocol</li> <li><strong>Part 3:</strong> Case study</li> </ul> <p><strong>Mini Quiz:</strong> Test Your Knowledge</p> <br> <p><em>Estimated time for completion: 50 minutes</em></p> </div> </div> </div> </div> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="7"> <div class="th">Session 6: Automation of the Mouse CFU Assay Using STEMvision™</div> </div> </div> <div class="tbody"> <div class="tr" row-num="8"> <div class="td top" valign="top"> <ul class="disc"> <li><strong>Part 1:</strong> Automation of the mouse CFU assay using STEMvision™</li> <li><strong>Part 2:</strong> Scoring mouse CFU: Results from STEMvision™</li> <li><strong>Part 3:</strong> Applications of STEMvision™ mouse analysis packages</li> </ul> <p><strong>Mini Quiz:</strong> Test Your Knowledge</p> <p><strong>Virtual Lab:</strong> <a href="https://www.youtube.com/playlist?list=PLSlDllUranmu2CU7ZAsHRhXfRMdra4UE7" target="_blank">Step-by-step narrated “How To” lab video demonstrations for STEMvision™</a></p> <br> <p><em>Estimated time for completion: 50 minutes</em></p> </div> </div> </div> </div> <div class="section table reformat-table mobile"> <div class="thead"> <div class="tr" row-num="5"> <div class="th">Final Assessment</div> </div> </div> <div class="tbody"> <div class="tr" row-num="6"> <div class="td top" valign="top"> <p>Complete this final assessment to test your knowledge and receive a certificate following successful completion.</p><br>  </div> </div> </div> </div> </div> </div>  <div class="general-disclaimer"> This on-demand course is divided into six sessions and a final assessment. Following completion of the course, trainees can choose to receive a certificate of completion. 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