Developing a Systemic Monoclonal Antibody Therapy for the Treatment of Large Burn Injuries

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Antibody Therapy for the Treatment of Large Burn Injuries</title> <meta name="description" content="Developing a Systemic Monoclonal Antibody Therapy for the Treatment of Large Burn Injuries"> <meta name="keywords" content="biodistribution, burn, flightless, systemic, fnAbs"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img 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publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Developing a Systemic Monoclonal Antibody Therapy for the Treatment of Large Burn Injuries</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alireza%20Hassanshahi">Alireza Hassanshahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Xanthe%20Strudwick"> Xanthe Strudwick</a>, <a href="https://publications.waset.org/abstracts/search?q=Zlatko%20Kopecki"> Zlatko Kopecki</a>, <a href="https://publications.waset.org/abstracts/search?q=Allison%20J%20Cowin"> Allison J Cowin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Studies have shown that Flightless (Flii) is elevated in human wounds, including burns, and reducing the level of Flii is a promising approach for improving wound repair and reducing scar formation. The most effective approach has been to neutralise Flii activity using localized, intradermal application of function blocking monoclonal antibodies. However, large surface area burns are difficult to treat by intradermal injection of therapeutics, so the aim of this study was to investigate if a systemic injection of a monoclonal antibody against Flii could improve healing in mice following burn injury. Flii neutralizing antibodies (FnAbs) were labelled with Alxa-Fluor-680 for biodistribution studies and the healing effects of systemically administered FnAbs to mice with burn injuries. A partial thickness, 7% (70mm2) total body surface area scald burn injury was created on the dorsal surface of mice (n=10/group), and 100碌L of Alexa-Flour-680-labeled FnAbs were injected into the intraperitoneal cavity (IP) at time of injury. The burns were imaged on days 0, 1, 2, 3, 4, and 7 using IVIS Lumina S5 Imaging System, and healing was assessed macroscopically, histologically, and using immunohistochemistry. Fluorescent radiance efficiency measurements showed that IP injected Alexa-Fluor-680-FnAbs localized at the site of burn injury from day 1, remaining there for the whole 7-day study. The burns treated with FnAbs showed a reduction in macroscopic wound area and an increased rate of epithelialization compared to controls. Immunohistochemistry for NIMP-R14 showed a reduction in the inflammatory infiltrate, while CD31/VEGF staining showed improved angiogenesis post-systemic FnAb treatment. These results suggest that systemically administered FnAbs are active within the burn site and can improve healing outcomes. The clinical application of systemically injected Flii monoclonal antibodies could therefore be a potential approach for promoting the healing of large surface area burns immediately after injury. <iframe src="https://publications.waset.org/abstracts/154516.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biodistribution" title="biodistribution">biodistribution</a>, <a href="https://publications.waset.org/abstracts/search?q=burn" title=" burn"> burn</a>, <a href="https://publications.waset.org/abstracts/search?q=flightless" title=" flightless"> flightless</a>, <a href="https://publications.waset.org/abstracts/search?q=systemic" title=" systemic"> systemic</a>, <a href="https://publications.waset.org/abstracts/search?q=fnAbs" title=" fnAbs"> fnAbs</a> </p> <a href="https://publications.waset.org/abstracts/154516/developing-a-systemic-monoclonal-antibody-therapy-for-the-treatment-of-large-burn-injuries" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/154516.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">172</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> 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Developing a Systemic Monoclonal Antibody Therapy for the Treatment of Large Burn Injuries