Mass spectrometry | Research equipment and services

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class="section__content"> </div> </div> <div id="block-uq-standard-theme-content" class="block block-system block-system-main-block"> <article about="/facilities/mass-spectrometry"> <div> <div class="uq-hero " data-gtm-category="Hero banner"> <div class="uq-container"> <div class="uq-hero__content"> <h1 class="uq-hero__title">Mass spectrometry</h1> </div> </div> </div> <div class="uq-container"> <div class="uq-sidebar-layout"> <div class="uq-sidebar-layout__sidebar"> <div id="local-nav-app"></div> </div> <div class="uq-sidebar-layout__main"> <div class="uq-section "> <p>Mass spectrometry has a range of applications, including molecular discovery, identification, characterisation and quantification. UQ has mass spectrometry equipment at a number of locations.</p> <div class="uq-accordion uq-accordion--is-manual" data-gtm-category="Accordion" role="tablist" aria-label="Accordion button group"> <div class="uq-accordion__item" role="presentation"> <button class="uq-accordion__toggle" data-gtm-no-link="data-gtm-no-link" aria-controls="accordion" aria-expanded="false" id="accordion-title"> <h2>Centre for Clinical Research (UQCCR)</h2> </button> <div class="uq-accordion__content" aria-hidden="true" id="accordion" aria-labelledby="accordion-title"> <h3>Services</h3> <p>The mass spectrometry facility at UQCCR is located on the Royal Brisbane and Women鈥檚 Hospital site at Herston and is NATA accredited for research, development, and evaluation (ISO/ IEC 17025).</p> <p>The MS facility team have extensive experience in a wide range of application areas including the identification of proteins/metabolites/lipids/small molecules, quantitation workflows including labelled and label free (SWATH and MRM), intact protein analysis and characterisation, and lipidomics workflows. The facility also provides training on sample preparation and the use of the specific instruments.</p> <h3>Equipment</h3> <ul> <li>SCIEX TripleTOF 5600 System x 2</li> <li>SCIEX QTRAP 5500 System</li> <li>SCIEX TOF/TOF 5800 System</li> <li>Shimadzu LCMS 8050 System</li> <li>Agilent 6490 System</li> <li>SelexION庐 Differential Ion Mobility (DMS)</li> <li>Shimadzu Nexera UHPLC Systems</li> <li>Eksigent NanoLC 400 Systems</li> <li>Eksigent MicroLC 200 System</li> <li>Agilent 1290 Infinity</li> <li>TriVersa Nanomate System</li> <li>Agilent 1100 HPLC systems with UV detector and fractionator</li> <li>Hamilton MicroLab庐 Star Robotic system</li> <li>Agilent 3100 Offgel Electrophoresis Fractionator</li> <li>Various software solutions for protein identification, quantitation of analytes, intact protein characterisation and lipid analysis</li> </ul> <h3>Contact</h3> <div class="highlight" data-gtm-category="Callout box"> <p><strong>Dr Sarah Reed</strong><br> Head, Mass Spectrometry Facility</p> <p>Website: <a href="https://clinical-research.centre.uq.edu.au/services/mass-spectrometry-services">Mass Spectrometry Facility</a><br> Phone: <a href="tel:+61733465007">07 3346 5007</a><br> Email: <a href="mailto:msf-uqccr@uq.edu.au">msf-uqccr@uq.edu.au</a><br> Location: Herston campus</p> </div> </div> </div> <div class="uq-accordion__item" role="presentation"> <button class="uq-accordion__toggle" data-gtm-no-link="data-gtm-no-link" aria-controls="accordion--2" aria-expanded="false" id="accordion--2-title"> <h2>Centre for Microscopy and Microanalysis (CMM) and Centre for Advanced Imaging (CAI)</h2> </button> <div class="uq-accordion__content" aria-hidden="true" id="accordion--2" aria-labelledby="accordion--2-title"> <h3>Services</h3> <p>Mass Spectrometry Imaging produces the spatial distribution of various analyte classes present in tissue samples mounted on microscope slides. By bringing MSI together with other imaging modalities such as microscopy and MRI, a more holistic picture of biomolecular imaging can be achieved.</p> <p>Within the facility we have a number of MALDI-TOF/TOF systems, and an atmospheric pressure MALDI which can be coupled to a QqTOF. We also have a JEOL MALDI Spiral-TOF, capable of higher mass resolving power (75,000). Another addition is a Bruker TIMS TOF Flex with MALDI-2, an instrument capable of both MALDI and Electrospray, making it a very useful instrument for Mass Spectrometry Imaging and companion LC-MS analysis.</p> <p>We have installed a Waters MRT, which is DESI and MALDI enabled, and offers mass resolving power in excess of 200k, making it a very powerful instrument for MS Imaging and LC-MS. The broad range of instruments allow us to perform Mass Spectrometry Imaging for a diverse range of analytes.</p> <p>In addition to mass spectrometers, we have a suite of supporting equipment including HPLC-spotting robots, and matrix deposition devices (both spray and vacuum sublimation based). A range of supporting software is also available, most of which can be accessed remotely.</p> <h3>Equipment</h3> <ul> <li>Bruker Autoflex Speed (MALDI-TOF/TOF)</li> <li>Burker Ultraflextreme (MALDI-TOF/TOF)</li> <li>Bruker MicroTOF Q II (QqTOF)</li> <li>Bruker TIMS TOF Flex with MALDI-2 (MALDI and ESI QqTOF with ion mobility)</li> <li>Waters MRT (MALDI and DESI)</li> <li>JEOL Spiral TOF (MALDI-TOF/TOF)</li> <li>Masstech AP-MALDI source</li> <li>Thermo nanoHPLC with Proteineer Spotting Robot</li> <li>Thermo HPLC with Thermo QqQ MS</li> <li>Bruker Imageprep</li> <li>Vacuum Sublimation Device</li> <li>Sunchrom Matrix Sprayer</li> <li>Sunchrom Digester</li> <li>Basic histology including microtome for MSI sample prep</li> <li>Software available includes Mascot, SCILs Lab, Flex Imaging, ClinProt Tools, PEAKS Studio X, Metaboscape</li> </ul> <h3>Contact</h3> <div class="highlight" data-gtm-category="Callout box"> <p><strong>Dr Brett Hamilton</strong><br> <br> Website: <a href="https://cmm.centre.uq.edu.au/about/cmm-facilities">Centre for Microscopy and Microanalysis</a><br> Phone: <a href="tel:+61406609223">0406 609 223</a><br> Email: <a href="mailto:cmm@uq.edu.au">cmm@uq.edu.au</a><br> Location: St Lucia campus</p> </div> </div> </div> <div class="uq-accordion__item" role="presentation"> <button class="uq-accordion__toggle" data-gtm-no-link="data-gtm-no-link" aria-controls="accordion--3" aria-expanded="false" id="accordion--3-title"> <h2>Institute for Molecular Bioscience (IMB)</h2> </button> <div class="uq-accordion__content" aria-hidden="true" id="accordion--3" aria-labelledby="accordion--3-title"> <h3>Services</h3> <p>IMB鈥檚 mass spectrometry facility is home to a suite of state-of-the-art mass spectrometry, high-performance liquid chromatography, and robotic instrumentation that have been refined and optimised to investigate biological systems in a high-throughput qualitative and quantitative manner.</p> <p>The 11 available systems within the facility provide researchers with the resources to investigate a broad range of mass spectrometric applications, including molecular discovery, identification, characterisation and quantification.</p> <h3>Equipment</h3> <ul> <li>SCIEX Triple TOF 6600 and 5600 Electrospray Triple TOF systems</li> <li>Agilent 6545 TOF Electrospray QqTOF</li> <li>SCIEX 5800 MALDI TOF-TOF</li> <li>SCIEX 4000 QTRAP Triple Quadrupole-Linear Ion Trap</li> <li>SCIEX 4000 Triple Quadrupole</li> </ul> <h3>Contact</h3> <div class="highlight" data-gtm-category="Callout box"> <p><strong>Alun Jones</strong><br> Facility Manager</p> <p>Website: <a href="https://imb.uq.edu.au/mass-spectrometry">Institute of Molecular Bioscience</a><br> Phone: <a href="tel:+61733462996">07 3346 2996</a><br> Email: <a href="mailto:a.jones@imb.uq.edu.au">a.jones@imb.uq.edu.au</a><br> Location: St Lucia campus</p> </div> </div> </div> <div class="uq-accordion__item" role="presentation"> <button class="uq-accordion__toggle" data-gtm-no-link="data-gtm-no-link" aria-controls="accordion--4" aria-expanded="false" id="accordion--4-title"> <h2>School of Chemistry and Molecular Biosciences (SCMB)</h2> </button> <div class="uq-accordion__content" aria-hidden="true" id="accordion--4" aria-labelledby="accordion--4-title"> <h3>Services</h3> <p>The SCMB mass spectrometry facility has a range of instruments for analysis for both biological and synthetic compounds, including proteomics and metabolomics, analysis of natural products, synthetic compounds.</p> <p>Analyses include accurate mass measurement (small molecules and intact proteins), quantitation (SWATH, ITRAQ, SILAC, MRM assays); identification of proteins and other compounds; characterisation of post-translational modifications on proteins (glycosylation, phosphorylation, methylation etc).</p> <h3>Equipment</h3> <ul> <li>Sciex ZenoTOF 7600 with Waters M-Class UPLC</li> <li>Sciex X500B QTOF with Exion UPLC</li> <li>Thermo Orbitrap Elite with ETD with Dionex UltiMate 3000 RSLCnanoHPLC</li> <li>Sciex TripleTof 5600 with Shimadzu Prominence nanoHPLC</li> <li>Sciex QTRAP 5500 with Shimadzu Nexera UPLC</li> <li>Bruker MicrOTof-Q with Dionex UltiMate 3000 HPLC</li> <li>Thermo LCQ Fleet ion trap</li> <li>ProteinPilot v5.0 software</li> <li>ProteomeDiscoverer v4.0 software</li> <li>PeakView v2.1 software with SWATH processing script</li> <li>Molecule Profiler software</li> <li>ProSight software</li> <li>Protein Deconvolution software</li> <li>Shimadzu Nexera UPLC system</li> <li>Agilent 1100/1200 capillary HPLC system</li> <li>Sciex OS-Q</li> <li>Sciex OS-MQ</li> <li>Byonic software</li> </ul> <h3>Contact</h3> <div class="highlight" data-gtm-category="Callout box"> <p><strong>Dr Amanda Nouwens</strong></p> <p>Website: <a href="https://scmb.uq.edu.au/">School of Chemistry and Molecular Biosciences</a><br> Phone: <a href="tel:+61733649490">07 3364 9490</a><br> Email: <a href="mailto:scmbms@uq.edu.au">scmbms@uq.edu.au</a><br> Location: St Lucia campus</p> </div> </div> </div> <div class="uq-accordion__item" role="presentation"> <button class="uq-accordion__toggle" data-gtm-no-link="data-gtm-no-link" aria-controls="accordion--5" aria-expanded="false" id="accordion--5-title"> <h2>Proteomics - Translational Research Institute (TRI)</h2> </button> <div class="uq-accordion__content" aria-hidden="true" id="accordion--5" aria-labelledby="accordion--5-title"> <h3>Services</h3> <p>The facility is available to research, clinical and industry users from TRI, UQ and the broader community. TRI Proteomics provides expert services in sample analysis, access to specialised equipment, as well as training and assistance.</p> <h3>Equipment</h3> <p>The following equipment is available to all users as part of the 'Core Facilities at TRI':</p> <ul> <li>Agilent 3100 Off-gel Electrophoresis (fractionator)</li> <li>Bioruptor homogeniser</li> <li>Direct detect (protein quantitation)</li> <li>Bravo robot (automated sample preparation)</li> <li>LC Q-exactive plus Mass Spectrometer</li> <li>LC Q-exactive HF Mass Spectrometer.</li> </ul> <p>The majority of equipment is TRI owned.</p> <h3>Contact</h3> <div class="highlight" data-gtm-category="Callout box"> <p>Website: <a href="https://www.tri.edu.au/proteomics">Translational Research Institute</a><br> Email: <a href="mailto:proteomiccore@tri.edu.au">proteomiccore@tri.edu.au</a><br> Location: Princess Alexandra Hospital campus</p> </div> </div> </div> </div> </div> </div> </div> </div> </div> </article> </div> </main> <footer 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