Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa by Sulforhodamine-B Assay-Guided Fractionation

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa by Sulforhodamine-B Assay-Guided Fractionation</title> <meta name="description" content="Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa by Sulforhodamine-B Assay-Guided Fractionation"> <meta name="keywords" content="Cytotoxic compounds, half-maximal inhibitory concentration, high-performance liquid chromatography, human HepG2 cells, Nigella sativa seeds, Sulforhodamine-B assay-guided fractionation."> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <meta name="citation_title" content="Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa by Sulforhodamine-B Assay-Guided Fractionation"> <meta name="citation_author" content="Harshani Uggallage"> <meta name="citation_author" content="Kapila D. Dissanayaka"> <meta name="citation_publication_date" content="2022/10/25"> <meta name="citation_journal_title" content="International Journal of Pharmacological and Pharmaceutical Sciences"> <meta name="citation_volume" content="16"> <meta name="citation_issue" content="10"> <meta name="citation_firstpage" content="186"> <meta name="citation_lastpage" content="190"> <meta name="citation_pdf_url" content="https://publications.waset.org/10012756/pdf"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value=""> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 33093</div> </div> </div> </div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa by Sulforhodamine-B Assay-Guided Fractionation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Harshani%20Uggallage">Harshani Uggallage</a>, <a href="https://publications.waset.org/search?q=Kapila%20D.%20Dissanayaka"> Kapila D. Dissanayaka</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>A Sulforhodamine-B assay-guided fractionation on Nigella sativa seeds was conducted to determine the presence of cytotoxic compounds against human hepatoma (HepG2) cells. Initially, a freeze-dried sample of Nigella sativa seeds was sequentially extracted into solvents of increasing polarities. Crude extracts from the sequential extraction of Nigella sativa seeds in chloroform and ethyl acetate showed the highest cytotoxicity. The combined mixture of these two extracts was subjected to bioassay guided fractionation using a modified Kupchan method of partitioning, followed by Sephadex® LH-20 chromatography. This chromatographic separation process resulted in a column fraction with a convincing IC50 (half-maximal inhibitory concentration) value of 13.07 µg/ml, which is considerable for developing therapeutic drug leads against human hepatoma. Reversed phase High-Performance Liquid Chromatography (HPLC) was finally conducted for the same column fraction and the result indicates the presence of one or several main cytotoxic compounds against human HepG2 cells. </p> <iframe src="https://publications.waset.org/10012756.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Cytotoxic%20compounds" title="Cytotoxic compounds">Cytotoxic compounds</a>, <a href="https://publications.waset.org/search?q=half-maximal%20inhibitory%20concentration" title=" half-maximal inhibitory concentration"> half-maximal inhibitory concentration</a>, <a href="https://publications.waset.org/search?q=high-performance%20liquid%20chromatography" title=" high-performance liquid chromatography"> high-performance liquid chromatography</a>, <a href="https://publications.waset.org/search?q=human%20HepG2%20cells" title=" human HepG2 cells"> human HepG2 cells</a>, <a href="https://publications.waset.org/search?q=Nigella%20sativa%20seeds" title=" Nigella sativa seeds"> Nigella sativa seeds</a>, <a href="https://publications.waset.org/search?q=Sulforhodamine-B%20assay-guided%20fractionation." title=" Sulforhodamine-B assay-guided fractionation."> Sulforhodamine-B assay-guided fractionation.</a> </p> <a href="https://publications.waset.org/10012756/investigation-of-cytotoxic-compounds-in-ethyl-acetate-and-chloroform-extracts-of-nigella-sativa-by-sulforhodamine-b-assay-guided-fractionation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10012756/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10012756/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10012756/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10012756/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10012756/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10012756/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10012756/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10012756/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10012756/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10012756/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10012756.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">446</span> </span> <p class="card-text"><strong>References:</strong></p> <br>[1] J. M. Pezzuto, “Plant-derived anticancer agents,” Biochemical Pharmacology, vol. 53, pp. 121-133, 1997. <br>[2] C. Y. Wang, X. Y. Bai, and C. H. Wang, “Traditional Chinese Medicine: A Treasured Natural Resource of Anticancer Drug Research and Development” The American Journal of Chinese Medicine, vol. 42, pp. 543-559, 2014. <br>[3] S. Iddamaldeniya, N. Wickramasinghe, I. Thabrew, N. Ratnatunge, and M. Thammitiyagodage, “Protection against diethylnitrosoamine-induced hepatocarcinogenesis by an indigenous medicine comprised of Nigella sativa, Hemidesmus indicus and Smilax glabra: a preliminary study,” Carcinogenesis, vol. 2, pp. 1-6, 2003. <br>[4] M. I. Thabrew, R. R. Mitry, M. A. Morsy, and R. D. Hughes, “Cytotoxic effects of a decoction of Nigella sativa, Hemidesmus indicus and Smilax glabra on human hepatoma HepG2 cells,” Life Sciences, vol. 77, pp. 1319-1330, 2005. <br>[5] S. R. Samarakoon, I. Thabrew, P. B. Galhena, D. De Silva, and K. H. Tennekoon, “A comparison of the cytotoxic potential of standardized aqueous and ethanolic extracts of a polyherbal mixture comprised of Nigella sativa (seeds), Hemidesmus indicus (roots) and Smilax glabra (rhizome),” Pharmacognosy research, vol. 2, pp. 335-342, 2010. <br>[6] M. T. Donato, A. Lahoz, J. V. Castell, and M. J. Gómez-Lechón, “Cell lines: a tool for in vitro drug metabolism studies,” Current Drug Metabolism, vol. 9, pp. 1-11, 2008. <br>[7] R. R. Mitry, C. E. Sarraf, R. Havlík, and N. A. Habib, “Detection of adenovirus and initiation of apoptosis in hepatocellular carcinoma cells after Ad-p53 treatment,” Hepatology, vol. 31, pp. 885-889, 2000. <br>[8] H. Jork, W. Funk, W. Fischer, H. Wimmer and D. T. Burns, “Thin-layer chromatography. Reagents and detection methods. Physical and chemical detection methods” VCH, Weinheim, (ISBN 3-527-27834-6), vol. 1, pp. 128-132, 1990. <br>[9] A. E. Cohen, and A. P. J. Fields, “The cat that caught the canary: what to do with single-molecule trapping,” ACS Nano, vol.57, pp. 5296-5299, 2011. <br>[10] B. Schuler, “Single-molecule FRET of protein structure and dynamics - a primer,” Journal of Nanobiotechnology, vol. 11, S2, 2013. <br>[11] K. D. Dissanayaka, B. K. Canfield and L. M. Davis, “Three-dimensional feedback-driven trapping of a single nanoparticle or molecule in aqueous solution with a confocal fluorescence microscope,” Optics Express, vol. 27, pp. 29759-29769, 2019. </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>

CINXE.COM

Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa by Sulforhodamine-B Assay-Guided Fractionation