Evaluation of Tryptic Peptides from Neisseria meningitidis O

<!DOCTYPE html> <html lang="es"> <head> <title>Evaluation of Tryptic Peptides from Neisseria meningitidis O | 6670</title> <meta name="keywords" content="Gail Whiting, Clive Metcalfe, Adrian F Bristow and Jun X Wheeler, "/> <meta name="description" content="Abstract Peptide-based protein quantitation using LC-MS/MS is a valuable method for the measurement of specific proteins in complex biological samples. To..6670"/> <meta name="citation_publisher" content="Ciencia y tecnología" /> <meta name="citation_journal_title" content="Revista de proteómica y enzimología"> <meta name="citation_title" content="Evaluation of Tryptic Peptides from Neisseria meningitidis Outer Membrane Proteins PorA and PorB Digestion, Peptides"> <meta name="citation_author" content="Gail Whiting" /> <meta name="citation_author" content="Clive Metcalfe" /> <meta name="citation_author" content="Adrian F Bristow" /> <meta name="citation_author" content="Jun X Wheeler" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="6"> <meta name="citation_issue" content="2"> <meta name="citation_abstract" content="Abstract Peptide-based protein quantitation using LC-MS/MS is a valuable method for the measurement of specific proteins in complex biological samples. To ensure accurate measurement of the target protein the signature peptides must be true stoichiometric representatives of the amount of protein in the sample. We report here the results of our evaluation of candidate signature peptides for IDMS quantitation of Neisseria meningitidis outer membrane proteins PorA and PorB. We observed considerable differences in the quantitation of the individual peptides and determined that the digestion protocol used was crucial to the accuracy of protein quantitation. A rapid rate of cleavage was essential to produce high quality data for the quantitation of PorA and PorB. This report also highlights the need for signature peptides and their isotopically labelled form, i.e. the internal standards, to be stable throughout digestion. However, we did observe that biased quantitation likely due to peptide instability could be mitigated by concurrent addition of the labelled internal standard peptide. Furthermore we observed that sample matrices have differing effects on specific peptide cleavage during protein digestion. In conclusion, the selection of stable signature peptides and the optimisation of the digestion conditions are not trivial considerations but are essential for accurate protein quantitation."> <meta name="citation_abstract_html_url" content="https://spanish.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html"> <meta itemprop="name" content="Ciencia y tecnología" /> <meta name="format-detection" content="telephone=no"> <meta http-equiv="Content-Language" content="es"> <meta name="google-site-verification" content="fht0Sbphb4JsUdVMASeyamGbjQ-E68ZABbBD_5ogQXY" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="ROBOTS" content="INDEX,FOLLOW"> <meta name="googlebot" content="INDEX,FOLLOW"> <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> <link rel="canonical" href="https://spanish.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html"> <link rel="alternate" href="https://spanish.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" hreflang="es-ES" /> <link href="https://cdn.jsdelivr.net/npm/bootstrap@5.2.3/dist/css/bootstrap.min.css" rel="stylesheet"> <link href="https://fonts.googleapis.com/css?family=Roboto:100,100i,300,300i,400,400i,500,500i,700,700i,900,900i" rel="stylesheet"> <link href="https://fonts.googleapis.com/css?family=Droid+Serif:400,400italic,700,700italic" rel="stylesheet" type="text/css"> <link rel="stylesheet" href="https://pro.fontawesome.com/releases/v5.10.0/css/all.css" crossorigin="anonymous" /> <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css"> <script src="https://code.jquery.com/jquery-3.6.0.min.js" type="text/javascript"></script> <script src="https://cdn.jsdelivr.net/npm/bootstrap@5.2.3/dist/js/bootstrap.bundle.min.js" type="text/javascript"></script> <script>fetch("https://ipinfo.io/json").then((t=>t.json())).then((t=>{if("IN"!=t.country){var e=document.createElement("script");e.src="https://pagead2.googlesyndication.com/pagead/js/adsbygoogle.js",e.setAttribute("data-ad-client","ca-pub-8046643215361735"),e.crossOrigin="anonymous",document.body.appendChild(e)}}));</script> <link rel="stylesheet" href="/assets/css/styles.css"> <script> $(document).ready(function() { // Explicitly initialize Bootstrap dropdowns var dropdownElementList = [].slice.call(document.querySelectorAll('.dropdown-toggle')); var dropdownList = dropdownElementList.map(function (dropdownToggle) { return new bootstrap.Dropdown(dropdownToggle); }); }); </script>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-GH5GSYYKJG"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-K9XP1YMFQL'); </script></head> <body> <header class="header-transparent header-transparent-bb header-sticky bg-color-white"> <div class="topbar-e theme-toggle-content bg-color-white"> <div class="container"> <div class="row"> <div class="col-sm-6"> <ul class="list-inline topbar-e-actions pull-left"> <li class="topbar-e-actions-item"><a class="topbar-e-actions-link" href="https://spanish.scitechnol.com/" title="haga clic aquí">Hogar</a></li> <li class="topbar-e-actions-item"><a class="topbar-e-actions-link" href="https://spanish.scitechnol.com/scitechnol-aboutus.html" title="haga clic aquí">Hogar</a></li> <li class="topbar-e-actions-item"><a class="topbar-e-actions-link" href="https://spanish.scitechnol.com/science-technology-journals-list.html" title="haga clic aquí">Revistas</a></li> <li class="topbar-e-actions-item"><a class="topbar-e-actions-link" href="https://spanish.scitechnol.com/submitmanuscript.html" title="haga clic aquí">Enviar manuscrito</a></li> <li class="topbar-e-actions-item"><a class="topbar-e-actions-link" href="https://spanish.scitechnol.com/contact.html" title="CiteScore de la revista">Contáctenos</a></li> </ul> </div> <div class="col-sm-4"> <ul class="list-inline text-right margin-t-5 margin-b-0"> <li class="animate-theme-icons"><a class="animate-theme-icons-body animate-theme-icons-dark-light-brd theme-icons-xs radius-circle" href="https://facebook.com/SciTechnol" target="_blank"> <i class="animate-theme-icons-element fab fa-facebook-f"></i></a> </li> <li class="animate-theme-icons"> <a class="animate-theme-icons-body animate-theme-icons-dark-light-brd theme-icons-xs radius-circle" href="https://twitter.com/SciTechnol" target="_blank"> <i class="animate-theme-icons-element fab fa-twitter"></i></a> </li> <li class="animate-theme-icons"> <a class="animate-theme-icons-body animate-theme-icons-dark-light-brd theme-icons-xs radius-circle" href="https://youtube.com/watch?v=2MXKrt2Rzx8" target="_blank"> <i class="animate-theme-icons-element fab fa-youtube"></i></a> </li> <li class="animate-theme-icons"> <a class="animate-theme-icons-body animate-theme-icons-dark-light-brd theme-icons-xs radius-circle" href="https://www.linkedin.com/in/international-journal-of-cardiovascular-research-59b8339a" target="_blank"> <i class="animate-theme-icons-element fab fa-linkedin-in"></i></a> </li> </ul> </div> <div class="col-sm-2">  <div class="nav-item dropdown multi_lang j_multi_lang mt-2"> <a class="nav-link dropdown-toggle" href="#" id="multi_lang" role="button" data-bs-toggle="dropdown" aria-expanded="false">Language</a> <ul class="dropdown-menu lang-drop-menu" aria-labelledby="multi_lang"> <li><a class="dropdown-item" href="https://www.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="English"> <img src="https://www.scitechnol.com/admin/flags/usa.png">English </a></li> <li><a class="dropdown-item" href="https://chinese.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Chinese"> <img src="https://www.scitechnol.com/admin/flags/china.png">Chinese </a></li> <li><a class="dropdown-item" href="https://russian.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Russian"> <img src="https://www.scitechnol.com/admin/flags/russia.png">Russian </a></li> <li><a class="dropdown-item" href="https://german.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="German"> <img src="https://www.scitechnol.com/admin/flags/germany.png">German </a></li> <li><a class="dropdown-item" href="https://french.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="French"> <img src="https://www.scitechnol.com/admin/flags/france.png">French </a></li> <li><a class="dropdown-item" href="https://japanese.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Japanese"> <img src="https://www.scitechnol.com/admin/flags/japan.png">Japanese </a></li> <li><a class="dropdown-item" href="https://portuguese.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Portuguese"> <img src="https://www.scitechnol.com/admin/flags/portugal.png">Portuguese </a></li> <li><a class="dropdown-item" href="https://hindi.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Hindi"> <img src="https://www.scitechnol.com/admin/flags/india.png">Hindi </a></li> <li><a class="dropdown-item" href="https://telugu.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Telugu"> <img src="https://www.scitechnol.com/admin/flags/india.png">Telugu </a></li> <li><a class="dropdown-item" href="https://tamil.scitechnol.com/abstract/evaluation-of-tryptic-peptides-from-neisseria-meningitidis-outer-membrane-proteins-pora-and-porbrndigestion-peptides-6670.html" title="Tamil"> <img src="https://www.scitechnol.com/admin/flags/india.png">Tamil </a></li> </ul> </div>  </div> </div> </div> </div> <div class="content-xs bg-color-teal-light"> <div class="container"> <div class="row"> <div class="col-sm-12"> <h1 class="font-size-30 fweight-400 color-white">Revista de proteómica y enzimología </h1> </div> </div> </div> </div> <nav class="navbar navbar-expand-lg bg-navbar navbar-light mega-menu journal-menu topbar-shadow" id="mainNavbar"> <div class="container"> <button class="navbar-toggler" type="button" data-bs-toggle="collapse" data-bs-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="collapse navbar-collapse justify-content-center" id="navbarSupportedContent"> <ul class="navbar-nav mb-auto mb-2 mb-lg-0"> <li class="nav-item"><a class="nav-link" href="https://spanish.scitechnol.com/journals/proteomics-enzymology.html" title="haga clic aquí"><i class="fa fa-home" aria-hidden="true"></i></a></li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown" role="button" data-bs-toggle="dropdown" aria-expanded="false">Instrucciones</a> <ul class="dropdown-menu" aria-labelledby="navbarDropdown"> <li><a class="dropdown-item" href="https://spanish.scitechnol.com/journals/instructionsforauthors-computer-engineering-and-information-technology.html" title="haga clic aquí">Para autor</a></li> <li><a class="dropdown-item" href="https://spanish.scitechnol.com/journals/ethics-proteomics-enzymology.html" title="haga clic aquí">Políticas y ética</a></li> <li><a class="dropdown-item" href="https://spanish.scitechnol.com/journals/aims-scope-proteomics-enzymology.html" title="haga clic aquí">Objetivo y alcance</a></li> <li><a class="dropdown-item" href="https://spanish.scitechnol.com/journals/peer-review-proteomics-enzymology.html" title="haga clic aquí">Revisión por pares</a></li> </ul> </li> <li class="nav-item"><a class="nav-link" href="https://spanish.scitechnol.com/journals/submitmanuscript-proteomics-enzymology.html" title="haga clic aquí">Enviar manuscrito</a></li> <li class="nav-item"><a class="nav-link" href="https://spanish.scitechnol.com/journals/archive-proteomics-enzymology.html" title="haga clic aquí">Artículos</a></li> <li class="nav-item"><a class="nav-link" href="" title="haga clic aquí">Pautas</a></li> <li class="nav-item"><a class="nav-link" href="https://spanish.scitechnol.com/journals/indexing-proteomics-enzymology.html" title="haga clic aquí">Indexación y archivo</a></li> <li class="nav-item"><a class="nav-link" href="https://spanish.scitechnol.com/contact.html" title="haga clic aquí">Contacto</a></li> </ul> </div> </div> </nav> </header> <div class="bg-color-sky-light"> <div class="content-md container border-bottom-1"> <div class="row"> <div class="col-sm-9"> <div class="margin-b-30"> <h2>Evaluation of Tryptic Peptides from Neisseria meningitidis Outer Membrane Proteins PorA and PorB Digestion, Peptides</h2> <p><i class="fas fa-user"></i> Gail Whiting, Clive Metcalfe, Adrian F Bristow and Jun X Wheeler</p> <p><p style="text-align: justify;"><strong>Abstract</strong></p> <p style="text-align: justify;">Peptide-based protein quantitation using LC-MS/MS is a valuable method for the measurement of specific proteins in complex biological samples. To ensure accurate measurement of the target protein the signature peptides must be true stoichiometric representatives of the amount of protein in the sample. We report here the results of our evaluation of candidate signature peptides for IDMS quantitation of Neisseria meningitidis outer membrane proteins PorA and PorB. We observed considerable differences in the quantitation of the individual peptides and determined that the digestion protocol used was crucial to the accuracy of protein quantitation. A rapid rate of cleavage was essential to produce high quality data for the quantitation of PorA and PorB. This report also highlights the need for signature peptides and their isotopically labelled form, i.e. the internal standards, to be stable throughout digestion. However, we did observe that biased quantitation likely due to peptide instability could be mitigated by concurrent addition of the labelled internal standard peptide. Furthermore we observed that sample matrices have differing effects on specific peptide cleavage during protein digestion. In conclusion, the selection of stable signature peptides and the optimisation of the digestion conditions are not trivial considerations but are essential for accurate protein quantitation.</p></p> <div class="alert alert-info text-left"><b>Descargo de responsabilidad:</b> este resumen se tradujo utilizando herramientas de inteligencia artificial y aún no ha sido revisado ni verificado.</div> </div> </div>  <div class="col-xs-12 col-md-3"> <div class="divider-v4 divider-v4-left-double bg-color-white padding-10 margin-b-10"> <h2 class="divider-v4-title">Explorar SciTechnol</h2> <div> <ul class="list-unstyled angle-right"> <li class="color-base"><a href="https://spanish.scitechnol.com/author-guidelines.html" title="haga clic aquí"> Pautas para el autor</a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/reviewer-guidelines.html" title="haga clic aquí"> Directrices para el revisor</a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scitechnol-associations-indexing.html" title="haga clic aquí"> Asociaciones</a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/submitmanuscript.html" title="haga clic aquí"> Enviar manuscrito</a></li> <li class="color-base"><a href="http://www.meetingsint.com/" title="haga clic aquí" target="_blank"> Conferencias</a></li> </ul> </div> </div>  <div class="divider-v4 divider-v4-left-double bg-color-white padding-10 margin-b-30"> <h2 class="divider-v4-title">Aspectos destacados de la revista</h2> <div class="height-300 scrollbar mCustomScrollbar _mCS_2 mCS-autoHide" style="overflow: visible; position: relative;"> <ul class="list-unstyled angle-right text-ellipsis"> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/system-biology-journals-articles-ppts-list.php"> Biología de sistemas </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/protein-biomarker-journals-articles-ppts-list.php"> Biomarcador de proteínas </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/enzyme-catalysis-journals-articles-ppts-list.php"> Catálisis enzimática </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/cofactors-journals-articles-ppts-list.php"> Cofactores </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/biomarker-discovery-journals-articles-ppts-list.php"> Descubrimiento de biomarcadores </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/enzyme-based-assays-journals-articles-ppts-list.php"> Ensayos basados en enzimas </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/enzyme-journals-articles-ppts-list.php"> Enzima </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/immobilized-enzymes-journals-articles-ppts-list.php"> Enzimas inmovilizadas </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/advanced-enzymology-journals-articles-ppts-list.php"> Enzimología avanzada </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/molecular-enzymology-journals-articles-ppts-list.php"> Enzimología molecular </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/protein-engineering-journals-articles-ppts-list.php"> Ingeniería de proteínas </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/metabolic-processes--journals-articles-ppts-list.php"> Procesos metabólicos </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/proteomics-journals-articles-ppts-list.php"> proteómica </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/clinical-proteomics-journals-articles-ppts-list.php"> Proteómica clínica </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/molecular-proteomics-journals-articles-ppts-list.php"> Proteómica molecular </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/proteome-journals-articles-ppts-list.php"> proteoma </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/structural-proteomics-journals-articles-ppts-list.php"> Structural Proteomics </a></li> <li class="color-base"><a href="https://spanish.scitechnol.com/scholarly/proteomic-technologies-journals-articles-ppts-list.php"> Tecnologías proteómicas </a></li> </ul> </div> </div> </div> </div> </div> </div>  <footer id="footer" class="footer"> <div class="container"> <div class="row margin-b-50"> <div class="col-sm-4 sm-margin-b-30"> <div class="footer-address"> <p class="footer-address-text">SciTechnol es una editorial en línea que disfruta de presencia global con revistas internacionales sobre clínica, medicina, medio ambiente, farmacia, neurociencias y gestión empresarial.</p> </div> <hr> <h2 class="footer-title">Síganos</h2> <ul class="list-inline ul-li-lr-0"> <li class="theme-icons-wrap"><a href="https://www.facebook.com/SciTechnol" target="_blank" rel="no follow"><i class="theme-icons theme-icons-white-bg theme-icons-sm radius-3 fab fa-facebook-f"></i></a></li> <li class="theme-icons-wrap"><a href="https://twitter.com/scitechnol3" target="_blank" rel="no follow"><i class="theme-icons theme-icons-white-bg theme-icons-sm radius-3 fab fa-twitter"></i></a></li> <li class="theme-icons-wrap"><a href="https://www.instagram.com/scitechnolpublisher/" target="_blank" rel="no follow"><i class="theme-icons theme-icons-white-bg theme-icons-sm radius-3 fab fa-instagram"></i></a></li> <li class="theme-icons-wrap"><a href="https://youtube.com/watch?v=2MXKrt2Rzx8" target="_blank" rel="no follow"><i class="theme-icons theme-icons-white-bg theme-icons-sm radius-3 fab fa-youtube"></i></a></li> <li class="theme-icons-wrap"><a href="https://www.linkedin.com/in/international-journal-of-cardiovascular-research-59b8339a" target="_blank" rel="no follow"><i class="theme-icons theme-icons-white-bg theme-icons-sm radius-3 fab fa-linkedin-in"></i></a></li> </ul> </div> <div class="col-sm-4"> <h3 class="footer-title">Etiquetas</h3> <ul class="list-inline footer-tags margin-b-30"> <li><a class="radius-50" href="https://spanish.scitechnol.com/science-technology-journals-list.html">Revistas</a></li> <li><a class="radius-50" href="https://spanish.scitechnol.com/submitmanuscript.html">Enviar manuscrito</a></li> <li><a class="radius-50" href="https://spanish.scitechnol.com/open-access.html">Acceso abierto híbrido</a></li> <li><a class="radius-50" href="https://spanish.scitechnol.com/contact.html">Contáctenos</a></li> </ul> <div> <div class="footer-copyright text-center margin-t-50 brd-color-white"> <p class="footer-copyright-item margin-b-0">© SciTechnol 2024. Todos los derechos reservados.</p> </div> </div> </div> <div class="col-sm-4"> <h3 class="footer-title">Datos de contacto</h3> <div class="footer-contact-info-bg"> <ul class="list-unstyled footer-contact-info"> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon fas fa-map-marker"></i> <div class="footer-contact-info-media"> <p class="footer-contact-info-text">40 Camino de Bloomsbury Planta baja Londres, Reino Unido WC1A 2SE</p> </div> </li> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon fa fa-phone"></i> <div class="footer-contact-info-media"> <p class="footer-contact-info-text">+44-203-769-1765</p> </div> </li> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon fab fa-whatsapp"></i> <div class="footer-contact-info-media"> <p class="footer-contact-info-text">+44-738-964-6375</p> </div> </li> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon fa fa-fax"></i> <div class="footer-contact-info-media"> <p class="footer-contact-info-text">+44-203-004-1157 </p> </div> </li> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon far fa-envelope-open"></i> <div class="footer-contact-info-media"> <a class="footer-contact-info-text" href="mailto:editorialoffice@scitechnol.com">editorialoffice@scitechnol.com</a> </div> </li> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon fa fa-link"></i> <div class="footer-contact-info-media"> <a class="footer-contact-info-text" href="https://spanish.scitechnol.com/">https://spanish.scitechnol.com/</a> </div> </li> <li class="footer-contact-info-item"> <i class="footer-contact-info-icon fab fa-twitter"></i> <div class="footer-contact-info-media"> <a class="footer-contact-info-text" href="https://twitter.com/scitechnol3">https://twitter.com/scitechnol3</a> </div> </li> </ul> </div> </div> </div> </div> </footer> </body> </html>

CINXE.COM

Evaluation of Tryptic Peptides from Neisseria meningitidis O | 6670