Isolation and Characterization of Actinophages Infecting Streptomyces scabies in Egypt

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of Actinophages Infecting Streptomyces scabies in Egypt</title> <meta name="description" content="Isolation and Characterization of Actinophages Infecting Streptomyces scabies in Egypt"> <meta name="keywords" content="potato scab, actinophages, biological control, electron microscopy, TIP, DEP, LIV, antiviral activity"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img 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publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Isolation and Characterization of Actinophages Infecting Streptomyces scabies in Egypt</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20Zahran">D. Zahran</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20AlKhazindar"> M. AlKhazindar</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Khalil"> M. Khalil</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20T.%20A.%20Sayed"> E. T. A. Sayed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Streptomyces scabies is a pathogenic actinomycete that infects potato crop causing severe production losses. Actinophages affect the composition and diversity of the bacterial population, thereby, can be used as a biological control. Samples of actinomycetes and phages were collected from different cultivated soils including farms of Faculty of Science, Faculty of Agriculture and different locations in Giza, Egypt. Actinomycetes were identified by using biochemical, morphological tests and molecular studies using 16S rRNA sequencing. Two specific phages (E1 and E2) against Streptomyces scabies and other hosts were isolated. Phages were identified using dilution end point (DEP), longevity in vitro (LIV), thermal inactivation point (TIP), host range and electron microscopy. PhageE1 was characterized by 10-8 (DEP),180 days(LIV), 95掳C(TIP), narrow host range and electron microscopy showed ahead (59.9 nm) and neck (10.4nm). On the other hand phageE2 had 10-20 (DEP),180 days(LIV), 90掳C(TIP), and the size of head was (67.2 nm) and tail (114nm). Antiviral activity was also studied using different chemicals (NaCL, KCL, CaCL2, BaCL2, CoCL2, AgNO3, ALCL3and HgCL2) with different concentrations and different plant extracts with different concentrations (star anise, tea, tillia, peppermint, ginger, cumin, chamomile, turmeric cinnamon, marjoram and black cumin). Both Phage E1and phage E2 were vulnerable to (cumin, ginger, chamomile, guavas leaves and star anise) but resistant to (Tillie, marjoram, fennelflower seeds, peppermint, and cinnamon). <iframe src="https://publications.waset.org/abstracts/34520.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=potato%20scab" title="potato scab">potato scab</a>, <a href="https://publications.waset.org/abstracts/search?q=actinophages" title=" actinophages"> actinophages</a>, <a href="https://publications.waset.org/abstracts/search?q=biological%20control" title=" biological control"> biological control</a>, <a href="https://publications.waset.org/abstracts/search?q=electron%20microscopy" title=" electron microscopy"> electron microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=TIP" title=" TIP"> TIP</a>, <a href="https://publications.waset.org/abstracts/search?q=DEP" title=" DEP"> DEP</a>, <a href="https://publications.waset.org/abstracts/search?q=LIV" title=" LIV"> LIV</a>, <a href="https://publications.waset.org/abstracts/search?q=antiviral%20activity" title=" antiviral activity"> antiviral activity</a> </p> <a href="https://publications.waset.org/abstracts/34520/isolation-and-characterization-of-actinophages-infecting-streptomyces-scabies-in-egypt" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34520.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">433</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a 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Isolation and Characterization of Actinophages Infecting Streptomyces scabies in Egypt