<?xml version="1.0" encoding="UTF-8"?> <article key="pdf/10002982" mdate="2015-10-01 00:00:00"> <author>Lilia M. Fernando and Matthew K. Vasher and Evangelyn C. Alocilja</author> <title>A DNABased Nanobiosensor for the Rapid Detection of the Dengue Virus in Mosquito</title> <pages>822 - 825</pages> <year>2015</year> <volume>9</volume> <number>12</number> <journal>International Journal of Pharmacological and Pharmaceutical Sciences</journal> <ee>https://publications.waset.org/pdf/10002982</ee> <url>https://publications.waset.org/vol/108</url> <publisher>World Academy of Science, Engineering and Technology</publisher> <abstract>This paper describes the development of a DNAbased nanobiosensor to detect the dengue virus in mosquito using electrically active magnetic (EAM) nanoparticles as concentrator and electrochemical transducer. The biosensor detection encompasses two sets of oligonucleotide probes that are specific to the dengue virus the detector probe labeled with the EAM nanoparticles and the biotinylated capture probe. The DNA targets are double hybridized to the detector and the capture probes and concentrated from nonspecific DNA fragments by applying a magnetic field. Subsequently, the DNA sandwiched targets (EAMdetector probe&amp;ndash; DNA target&amp;ndash;capture probebiotin) are captured on streptavidin modified screen printed carbon electrodes through the biotinylated capture probes. Detection is achieved electrochemically by measuring the oxidation&amp;ndash;reduction signal of the EAM nanoparticles. Results indicate that the biosensor is able to detect the redox signal of the EAM nanoparticles at dengue DNA concentrations as low as 10 ng&amp;mu;l.</abstract> <index>Open Science Index 108, 2015</index> </article>