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CD11b Monoclonal Antibody (M1/70), Super Bright™ 600 (63-0112-82)
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<div class="account-dd"> <div class="account"> <div class="cmp-p-ctaitem m--btn-primary m--block"> <div class="cmp-ctaitem"> <a class="cmp-ctaitem__anchor" href="/auth/initiate"> <span class="cmp-ctaitem__text">Sign in</span> </a> </div> </div> <div class="create-account pt-l1 pb-l1 border-bottom">Don't have an account ? <a href="/auth/create-account">Create Account</a> </div> <div class="create-account"> <ul> <li class="loyalty-text"><a href="#"></a></li> <li class="points"><a href="#"></a></li> <li> <a href="/auth/initiate">Account</a> </li> <li> <a href="/store/orders/solu">Check Order Status</a> </li> <li> <a href="https://apps.thermofisher.com ">Connect: Lab, Data, Apps</a> </li> <li> <a href="/auth/initiate">Custom Products & Projects</a> </li> <li> <a href="/auth/initiate">Instrument Management</a> </li> </ul> <!-- CMGT DOMAIN: /order/catalog --> </div> </div> </div> <!-- performance-begin --> <script type="text/javascript"> window.performance && window.performance.mark && 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468bd07150dbbf Source: /tf/js/dist/digitaldata.js */ (function () { "use strict"; /** * Invoking a Function as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script> <!-- performance-begin --> <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. Please try again later.", "MINICART_ERROR_2" :"Please enter a quantity in the range of 1 to 999.", "MINICART_ERROR_3" :"Could not add item(s) to cart.", "MINICART_ERROR_4" :"Could not remove item from cart.", "MINICART_ERROR_5" :"Could not create/restore cart.", "MINICART_ERROR_6" :"Unable to retrieve cart details. Please try again later.", "MINICART_MESSAGE_1" :"Adding item(s) to cart...", "MINICART_MESSAGE_2" :"Removing item(s) from cart...", "MINICART_CART_NAME_LABEL_1" :"Recently added to the", "MINICART_NOTE_1" : "Your total price and product details can be viewed in the cart.", MINICART_SUMMARY : "Cart summary", MINICART_PRICE : "Price", MINICART_PRODUCT : "Product", LIST_PRICE : "Price", YOUR_PRICE : "Your price", WEB_PRICE: "Online offer", PRICE_ENDS: "ends", SIGN_IN: "Sign in", //New mini cart CATALOG_NUMBER: "Catalog number:", CONTACT_US: "Contact us", RFQ: "Request quote", LEARN_WHERE_TO_BUY: "Learn where to buy", CURRENCY: "USD", //End new mini cart "CHANGE_CART" :"Change cart", "VIEW_ALL" :"View All", "ITEM" :"item", "ITEMS" :"items", "CLOSE" :"Close", "SUBTOTAL" :"Subtotal", "TOTAL" :"Total", "MANAGE_CART" :"Manage Cart", "FIELD" :"field", "GLOBAL_ERROR_1" :"Please choose one or both of the options for", "GLOBAL_ERROR_2" :"is not a valid e-mail address.", "GLOBAL_ERROR_3" :"Unsupported character(s) were found in field", "GLOBAL_ERROR_4" :"and will be replaced with plain text equivalents (where available). Please review the changes and try again.", "GLOBAL_ERROR_5" :"Please fill in the", "GLOBAL_ERROR_6" :"Quantities must be whole numbers greater than or equal to zero.", "GLOBAL_ERROR_7" :"is either not a number or a number less than zero, please provide a numerical quantity", "GLOBAL_ERROR_8" :"Reserved character | not allowed. Please remove and try again.", "required" : "One or more required fields are missing", "requireOneOfMany" : "At least one field in the group must be filled out", "password" : "Password does not conform to the password policy", "passwordConfirm" : "Password and Confirm Password do not match", "emailConfirm" : "Email and Confirm Email do not match", "secretConfirm" : "Secret Answer and Confirm Secret Answer do not match", "alphanumeric" : "Value must be alphanumeric", "phone" : "Phone number or Fax number provided were non-numeric values", "email" : "Email address provided is in an invalid format", "date" : "Date is in an invalid format (ex. MM/DD/YYYY)", "expirationDate" : "Expiration date provided is invalid", "currency" : "Currency is in an invalid format", "creditCard" : "Credit card number is invalid", "quantity" : "Quantity must in the range of 1 - 999", "acceptance" : "The terms and conditions must be accepted to continue", "checkboxes" : "At least one item must be selected", "twoOrMorecheckboxes" : "Merging carts requires multiple carts to be selected", "file" : "A file must be selected to upload", "NEW_USER" :"New user", "REGISTER_NOW" :"Register Now", "LOGIN_WIDGET_MESSAGE1" :"View your contract prices and real-time product availability.", "WELCOME" :"Welcome", "MY_ACCOUNT" :"My Account", "FAVORITES" :"Favorites", "SAVED_LISTS" :"Saved Lists", "ORDER_STATUS" :"Order Status", "LOGOUT" :"Logout", "APPROVALS" :"Approvals", "REMOVE" :"Remove", "QUANTITY" :"Qty", "EDIT" :"Edit", "SEARCH_BOX_TEXT" :"",//"Search by catalog number or keyword", "SELECT_LANGUAGE" :"Select a Language", "MY_MESSAGES" :"My Messages", "MESSAGE_ALERTS" :"Message Alerts", "B2B_NEW_WINDOW_ALERT" :"You are about to open a new browser window. \nClicking an external link on our site causes a new window to open. However, your shopping session will remain active in this window.Simply return here to continue shopping. \n Would you like to proceed?", "GREETING" :"", "SCMS_SHIP_TO_LAB" :"Ship To Lab", "SCMS_SHIP_TO_SC" :"Supply Center Order", "SCMS_SHIP_TO_SC_FOR_EU":"Supply Centre Order", "SUBMIT" :"Submit", "SUBMIT_ERROR_VALIDATE_EMAIL":"Sorry - we could not validate your email address. Please try again. ", "SUBMIT_ERROR_SERVER" :"We had an issue handling your request - please try again later.", "RECENT_SEARCHES" :"Recent searches", "inText" :"in", "searchSuggestions" :"Search suggestions", "RECENTLYVIEWED_TITLE" : "Recently Viewed Items", "RECENTLYVIEWED_DELETE" : "Remove", "RECENTLYVIEWED_TODAY" : "Today", "RECENTLYVIEWED_PAGINATE": "{C} of {T}", DF_MONTH_0:"January", DF_MONTH_1:"February", DF_MONTH_2:"March", DF_MONTH_3:"April", DF_MONTH_4:"May", DF_MONTH_5:"June", DF_MONTH_6:"July", DF_MONTH_7:"August", DF_MONTH_8:"September", DF_MONTH_9:"October", DF_MONTH_10:"November", DF_MONTH_11:"December", DF_WEEKDAY_0:"Sun", DF_WEEKDAY_1:"Mon", DF_WEEKDAY_2:"Tue", DF_WEEKDAY_3:"Wed", DF_WEEKDAY_4:"Thu", DF_WEEKDAY_5:"Fri", DF_WEEKDAY_6:"Sat", DF_FORMAT:"{0}, {2} {1}" }; var localizedImage = { "LOGIN_WIDGET_EXPAND":"/shared-static/images/buttons/loginWidgetExpand.gif", "LOGIN_WIDGET_MY_ACCOUNT_EXPAND":"/shared-static/images/buttons/loginWidgetMyAccountExpand.gif", "LOGIN_WIDGET_CONTRACT":"/shared-static/images/buttons/loginWidgetContract.gif", "LOGIN_WIDGET_MY_ACCOUNT_CONTRACT":"/shared-static/images/buttons/loginWidgetMyAccountContract.gif", "LOGOUT_BUTTON":"/shared-static/images/login/useraccount.gif", "CLOSE_SESSION_COOL":"/shared-static/images/login/Close-Session-Cool.JPG", "ICON_CLOSE":"/shared-static/images/login/icon_close.GIF" }; var localizedLink = { "B2B_CHECK_OUT_LINK":"/en/US/procurement/lt?cmd=ViewCart&ShoppingCartKey=", "B2B_MANAGE_CART_LINK":"/en/US/procurement/lt?cmd=OILDataDisplay&ShoppingCartKey=", "B2B_VIEW_ALL_LINK":"/en/US/procurement/lt?cmd=ViewCart&ShoppingCartKey=", "LIGGED_IN_CHECKOUT_LINK":"/en/US/direct/lt?cmd=ViewCart&ShoppingCartKey=", "LOGGED_IN_MANAGE_CART_LINK":"/en/US/direct/lt?cmd=OILDataDisplay&ShoppingCartKey=", "LOGGED_IN_VIEW_ALL_LINK":"/en/US/direct/lt?cmd=ViewCart&ShoppingCartKey=", "ANONYMOUS_CHECKOUT_LINK":"/en/US/adirect/lt?cmd=ViewCart&ShoppingCartKey=", "ANONYMOUS_MANAGE_CART_LINK":"/en/US/adirect/lt?cmd=OILDataDisplay&ShoppingCartKey=", "ANONYMOUS_VIEW_ALL_LINK":"/en/US/adirect/lt?cmd=ViewCart&ShoppingCartKey=", "PRODUCT_DETAIL_URL_B2B":"/en/US/procurement/lt?cmd=catProductDetail&productID=", "PRODUCT_DETAIL_URL_ANONYMOUS":"/en/US/adirect/lt?cmd=catProductDetail&productID=", "PRODUCT_DETAIL_URL_LOGGED_IN":"/en/US/direct/lt?cmd=catProductDetail&productID=", "MY_ACCOUNT_LINK_CSR":"/en/US/enterpriseMgr/lt?cmd=HomeDataDisplay", "QUICK_ORDER_ANONYMOUS":"/en/US/adirect/lt?cmd=IVGNQuickOrderLanding", "MY_ACCOUNT_LINK_B2B":"/en/US/procurement/lt?cmd=PartnerSysUserMyAccount", "ORDER_STATUS_LINK_B2B":"/en/US/procurement/lt?cmd=WorkspaceDataDisplay&_Tab=Ordered", "MY_ACCOUNT_LINK":"/en/US/direct/lt?cmd=PartnerSysUserMyAccount", "ORDER_STATUS_LINK":"/en/US/direct/lt?cmd=WorkspaceDataDisplay&_Tab=Ordered", "COMERGENT_CLOSE_SESSION_LINK":"/en/US/procurement/lt?cmd=PunchInOrder&mode=Cancel", "REGISTRATION_URL":"/en/US/adirect/lt?cmd=FullRegistrationData&GroupKey=103&Command=updateExecute&anonymousHomePageMsg=OnlineOrderingPageDisplay", "LIGGED_IN_CHECKOUT_LINK_SCMS":"/en/US/direct/lt?cmd=ViewCart&ShoppingCartKey=", "SCMS_CHECKOUT_SC":"/en/US/procurement/lt?cmd=SetSCMSAddress", "SCMS_CHECKOUT":"/en/US/procurement/lt?cmd=ViewCart&ShoppingCartKey=", "PSCMS_CHECKOUT_SC":"/en/US/procurement/lt?cmd=PSCMSCheckout", "GUEST_USER_AUTH_REDIRECT":"/en/US/procurement/lt?cmd=B2BMasterUserRegistration&operation=identifyUser", "GUEST_USER_AJAX_AUTH":"/en/US/procurement/lt?cmd=B2BMasterUserRegistration&operation=validateNLogin", "GUEST_USER_AJAX_AUTH_UNIQUE_EXPRESS":"/en/US/procurement/lt?cmd=ExpressUniqueUserEmailUpdate", "GUEST_USER_SEARCH_TOOL":"/en/US/direct/lt?cmd=WorkspaceDataDisplay&_Tab=Ordered" }; 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Delete this commented message after PDP redesign is completed --> <div block-ui="main" class="block-ui-main"></div> <div class="hero hero-loading-height" ng-class="{'is-loaded':$ctrl.isLoaded, 'with-banner': 'false' == 'true'}"> <div class="container"> <div class="hero-content"> <div class="brand-breadcrumb to-hide-on-mobile row"> <div class="large-span-12 medium-span-12 target_page_breadcrumbs"> <div class="breadcrumb breadcrumb-hide-restricted-only"> <ul class="breadcrumb breadcrumb-hide-restricted-only hero-breadcrumbs"> <li class="active"> <a href="/us/en/home/life-science/antibodies/primary-antibodies.html" target="_self"> Primary Antibodies </a> <span class="divider">›</span> </li> <li class="active"> <a href="/antibody/primary/target/cd11b" target="_self" class="targetLink"> CD11b Antibodies</a> </li> </ul> </div> </div> </div> <svg xmlns="http://www.w3.org/2000/svg" display="none"> <symbol id="close-drawer-icon" viewBox="0 0 22.74 22.74"> <line x1="1.67" y1="1.67" x2="21.33" 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2.048 0.896 2.048 2.048 0 1.088-0.96 2.048-2.048 2.048zM17.792 22.976c0 0.768-0.064 1.152-0.256 1.536-0.256 0.576-0.832 0.896-1.6 0.896-0.704 0-1.28-0.32-1.6-0.896-0.192-0.384-0.256-0.768-0.256-1.536v-8.256c0-0.768 0.064-1.152 0.256-1.536 0.256-0.576 0.832-0.896 1.6-0.896 0.704 0 1.28 0.32 1.6 0.896 0.192 0.384 0.256 0.768 0.256 1.536v8.256zM16 0.64c-8.512 0-15.36 6.848-15.36 15.36s6.848 15.36 15.36 15.36c8.512 0 15.36-6.848 15.36-15.36s-6.848-15.36-15.36-15.36z"></path> </symbol> </svg> <div class="brand-info"> <p class="brand-name" itemprop="brand">Invitrogen</p> </div> <h1 class="product-name">CD11b Monoclonal Antibody (M1/70), Super Bright™ 600, eBioscience™</h1> <div class="details-hero" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationPublishedFigures(event, this)"> <a class="link published-link animated-scroll " href="#image-gallery" onclick="window.THERMO_FISHER.pdp.inp.publishedFiguresHero(event, this)"> <svg class="svg-icon published-figures-icon"> <use 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href="https://www.thermofisher.com/global/en/home/life-science/antibodies/antibodies-learning-center/antibody-protocols.html?icid=antibody-pdp-protocols" target="_blank" class="link-antibody"> <span> <svg class="svg-icon protocols-icon"> <use href="#icon-protocols"></use> </svg> </span> Protocols </a> </div> <div class="link-container"> <a href="/us/en/home/technical-resources/contact-us.63-0112-82.html?supportType=TS" class="link-antibody"> <span> <svg class="svg-icon tech-support-icon"> <use href="#icon-tech-support"></use> </svg> </span> Tech Support </a> </div> </div> </div> <div class="cmrc-card-cnt" id="antibody_primary"> <div class="price-card-alignmnet" tf-product-order> <aside class="sidecard"> <div ng-switch on="$productOrderCtrl.isMultiPack()"> <div ng-switch-when="true"> <div ng-include="$productOrderCtrl.getTemplatePath()+'product-order-multipack.template.html'"></div> </div> <div ng-switch-default> <div ng-include="$productOrderCtrl.getTemplatePath()+'product-order-singlepack.template.html'"></div> </div> </div> </aside> </div> </div> </div> <div id="createCitationModal" class="modal fade citation-modal" role="dialog" aria-labelledby="createCitationModal-title"> <!-- Modal content--> <div class="modal-content" role="document"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal">×</button> <h4 class="modal-title" id="createCitationModal-title">Cite CD11b Monoclonal Antibody (M1/70), Super Bright™ 600, eBioscience™</h4> </div> <div class="modal-body"> <textarea id="citationTextArea" rows="5">The following product was used in this experiment: CD11b Monoclonal Antibody (M1/70), Super Bright™ 600, eBioscience™ from Thermo Fisher Scientific, catalog # 63-0112-82, RRID AB_2637408.</textarea> </div> <div class="modal-footer"> <!--[if lte IE 9]> <h4>Copy to clipboard.</h4> <span style="display: none;"> <![endif]--> <button type="button" class="well-red btn 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( A ) I. Upper panel: TIM3 mRNA expression in RAW264.7 cells and in ATC xenografts. Total RNA was isolated, and the mRNA expression of TIM3 was examined by RT-PCR analysis. Lane 1, RAW264.7 cells; lanes 2-4, 8505C xenograft tumors ( n \u003d 3); lanes 5-7, C643 xenograft tumors ( n \u003d 3). The lower panel represents the expression of actin. II. TIM3 mRNA expression was normalized by beta-actin. Densitometry analysis of TIM3 is shown. Representative results of 3 independent experiments. ( B - D ) 20X and 63X images of 5 um thick 8505C xenograft tumor sections immunostained with TIM3-PE antibody (red). Nuclei were counterstained with DAPI. Representative immunofluorescence images of 3 independent experiments. ( E - G ) Representative FACS analysis of 2 independent experiments of macrophages (CD11b + F4/80 + TIM3 + ) out of all CD45 + cells in the 8505c xenograft tumors ( E ) and C643 xenograft tumors ( F ). ( G ) Quantification of the percentage of TIM3 + cells ( n \u003d 6-8).','shortDescription':'Figure 4 TIM3-positive macrophages were recruited to ATC xenograft tumors. ( A ) I. Upper panel: TIM3 mRNA expression in RAW264.7 cells and in ATC xenografts. Total RNA was isolated, and the mRNA expression of TIM3 was examined by RT-PCR analysis. Lane 1, RAW264.7 cells; lanes 2-4, 8505C xenograft tumors ( n \u003d 3); lanes 5-7, C643 xenograft tumors ( n \u003d 3). The lower panel represents the expression of actin.... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Nov 3, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Biology','journalText':'Biology 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24742_biology-11-01609-g004.jpg','benchSciPubmedId':'36358310'},{'sku':'63-0112-82','imageId':'1023601','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24738_urn-x-wiley-26882663-media-mco2193-mco2193-fig-0004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24738_urn-x-wiley-26882663-media-mco2193-mco2193-fig-0004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24738_urn-x-wiley-26882663-media-mco2193-mco2193-fig-0004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24738_urn-x-wiley-26882663-media-mco2193-mco2193-fig-0004.jpg?time\u003d20240507','sortOrder':'3','description':'4 FIGURE Deciphering the diversity of myeloid cells in infected lung lesions. (A) Uniform manifold approximation and projection (UMAP) plot of the defined myeloid cell subsets in the six samples. (B) UMAP plot of the myeloid cell subsets in the three groups. (C) Bar plot showing the relative proportions of identified cell populations in each sample, colored according to cluster designation. Identified cell types are shown on the right. (D) Dotplot of the top expressed genes distinctly within the subclusters of myeloid cells. Avg. Exp: average expression; % of Exp: percent of expression. (E) Immunofluorescence (IF) of neutrophils with 4\u0026apos;,6-diamidino-2-phenylindole (DAPI) (blue) and myeloperoxidase (MPO) (red) colored among the three groups is shown. Magnification: 20x. Scale bar \u003d 100 mum. (F and G) Multiplex immunohistochemistry (mIHC) showing coexpression of CD11c (green), Siglec-F (red), and DAPI (blue)-positive cells called alveolar macrophages (AMs) (F) and CD11b (green), Mertk (red), and DAPI (blue)-positive cells called interstitial macrophages (IMs) (G) among the three groups. Magnification: 20x. Scale bar \u003d 100 mum. (H-J) Representative flow cytometry plots and quantification showing the proportional changes in neutrophils (H), AMs (I), and IMs (J) in the three groups. (K) Histograms showing the proportion of neutrophils, AMs and IMs among the three groups ( n \u003d 3). Bars represent mean +- SD. Significant differences were designated by using analysis of variance (','shortDescription':'4 FIGURE Deciphering the diversity of myeloid cells in infected lung lesions. (A) Uniform manifold approximation and projection (UMAP) plot of the defined myeloid cell subsets in the six samples. (B) UMAP plot of the myeloid cell subsets in the three groups. (C) Bar plot showing the relative proportions of identified cell populations in each sample, colored according to cluster designation. Identified cell ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Dec 1, 2022, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'MedComm','journalText':'MedComm 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24738_urn-x-wiley-26882663-media-mco2193-mco2193-fig-0004.jpg','benchSciPubmedId':'36514779'},{'sku':'63-0112-82','imageId':'1018050','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24742_biology-11-01609-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24742_biology-11-01609-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24742_biology-11-01609-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24742_biology-11-01609-g003.jpg?time\u003d20240507','sortOrder':'4','description':'Figure 3 M2-like macrophages were recruited to ATC xenograft tumors. ( A , B ) Representative FACS analysis of macrophages (CD11b + F4/80 + ) out of all CD45 + cells infiltrating 8505C xenograft tumors ( A ) and C643 xenograft tumors ( B ). ( C ) Quantification of the percentage of CD11b + F4/80 + cells ( n \u003d 8-10). ( D , F ) Representative FACS analysis of M2-macrophages (CD11b + F4/80 + CD206 + ) out of all CD45 + cells infiltrating 8505C xenograft tumors ( D ) and C643 xenograft tumors ( E ). ( F ) Quantification of the percentage of CD11b + F4/80 + CD206 + cells ( n \u003d 3-5). ( G , H ) Representative histograms of Arg1 ( G ) and iNOS ( H ) expression in CD206 low/int (blue histograms) and CD206 high (pink histograms) populations in total cells from C643 xenograft tumors. ( I ) Mean fluorescence intensity (MFI) of Arg1/iNOS ratio in the CD206 high group and in the CD206 low/int group ( n \u003d 8-10). Data are expressed as mean +- SEM of 3 independent experiments. ** p \u003c 0.005.','shortDescription':'Figure 3 M2-like macrophages were recruited to ATC xenograft tumors. ( A , B ) Representative FACS analysis of macrophages (CD11b + F4/80 + ) out of all CD45 + cells infiltrating 8505C xenograft tumors ( A ) and C643 xenograft tumors ( B ). ( C ) Quantification of the percentage of CD11b + F4/80 + cells ( n \u003d 8-10). ( D , F ) Representative FACS analysis of M2-macrophages (CD11b + F4/80 + CD206 + ) out of a... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Nov 3, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Biology','journalText':'Biology 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24742_biology-11-01609-g003.jpg','benchSciPubmedId':'36358310'},{'sku':'63-0112-82','imageId':'878921','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14985_pone.0255296.g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14985_pone.0255296.g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14985_pone.0255296.g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14985_pone.0255296.g003.jpg?time\u003d20240507','sortOrder':'5','description':'10.1371/journal.pone.0255296.g003 Fig 3 Chronodisruption causes increased macrophage and microglial signaling in the placenta. Placentas from ( A-C ) control light (CL) and mice exposed to ( D-F ) developmental chronodisruption (CD) were labeled for inflammatory markers labeling microglia and macrophages within the decidual zone. In placenta from CD mice increased placental ( G ) Iba1 fluorescence (Student\u0027s 2-tailed unpaired t-test, t \u003d 2.49, df \u003d 8, p \u003d 0.038) and increased placental (H ) CD11b fluorescence (Student\u0027s 2-tailed unpaired t-test, t \u003d 2.70, df \u003d 8, p \u003d 0.027) were detected. CL \u003d 4 placenta from different dams, 3 images each; CD \u003d 6 placenta from different dams, 3 images each. All data are presented as mean +- SEM, scale bar \u003d 20 microns, and * \u003d p\u003c0.05.','shortDescription':'10.1371/journal.pone.0255296.g003 Fig 3 Chronodisruption causes increased macrophage and microglial signaling in the placenta. Placentas from ( A-C ) control light (CL) and mice exposed to ( D-F ) developmental chronodisruption (CD) were labeled for inflammatory markers labeling microglia and macrophages within the decidual zone. In placenta from CD mice increased placental ( G ) Iba1 fluorescence (Student\u0027... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 24, 2021, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14985_pone.0255296.g003.jpg','benchSciPubmedId':'34370755'},{'sku':'63-0112-82','imageId':'878830','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14991_fcvm-08-649124-g0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14991_fcvm-08-649124-g0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14991_fcvm-08-649124-g0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14991_fcvm-08-649124-g0003.jpg?time\u003d20240507','sortOrder':'6','description':'Figure 3 Histochemical analysis of eye cross-sections show reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. (A) Histochemical analysis of eye cross-sections shows reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. Enlarged images of the corneal region of two mice visibly show more swelling and a higher presence of inflammatory cells in the control mice. Inflammatory cell nuclei are stained by the deep-purple spots marked with white triangles in the light pink stromal layer of the cornea. (B) To further identify infiltrating immune cells, the slides were stained with CD11b (an immune cell marker, green). (C) CD11b positive cells (cells/0.1 mm 2 ) were quantified ( n \u003d 3/group; * p \u003c 0.05).','shortDescription':'Figure 3 Histochemical analysis of eye cross-sections show reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. (A) Histochemical analysis of eye cross-sections shows reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. Enlarged images of the corneal region of two mice visibly show more swelling and a higher presence... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))','title':'CD11b Antibody (63-0112-82) in ICC/IF, IHC (PFA)','appAbv':'IHC (PFA)','dateTime':'May 20, 2022, 4:00:00 AM','appName':'Immunohistochemistry (PFA fixed)','type':'Published Figures','journalTitle':'Frontiers in cardiovascular medicine','journalText':'Frontiers in cardiovascular medicine 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14991_fcvm-08-649124-g0003.jpg','benchSciPubmedId':'34164439'},{'sku':'63-0112-82','imageId':'876824','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14925_gr8.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14925_gr8.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14925_gr8.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14925_gr8.jpg?time\u003d20240507','sortOrder':'7','description':'Figure 8 Absence of IDO contributed to increased CD8 + T infiltration in colon. ( A ) Absolute numbers of CD4 + and CD8 + T cells infiltrated in tumor were measured by flow cytometry (n \u003d 6). ( B ) The percentage of CD4 + and CD8 + T cells in spleen lymphocytes was analyzed by flow cytometry (n \u003d 6). ( C , D ) Absolute numbers of CD4 + and CD8 + T cells in spleen lymphocytes were detected by flow cytometry (n \u003d 6). ( E , F ) The frequency of intratumoral CD8 + / CD69 + T cells was determined (n \u003d 6). ( G , H ) The percentage of CD8 + GZB + T cells was detected (n \u003d 6). ( I , J ) The expression of Ki67 and its mean fluorescence intensity (MFI) of CD8 + T were shown (n \u003d 6). ( K , L ) Annexin V expression on intratumoral CD8 + T cells and its mean fluorescence intensity were determined by flow cytometry (n \u003d 6). ( M , N ) Serial sections of colon tissues were immunostained with DAPI (blue), anti F4/80 (green), and CD8 (red). They were observed by confocal laser scanning microscope (scale bar \u003d 50 mum, 10 mum). Expressions of CD11b (red) and CD4 (green) were analyzed by immunofluorescence cytochemistry (scale bar \u003d 50 mum, 10 mum). The results are representative of at least 6 independent experiments and expressed as mean +- SD. * P \u003c .05, ** P \u003c .01 indicates significant difference.','shortDescription':'Figure 8 Absence of IDO contributed to increased CD8 + T infiltration in colon. ( A ) Absolute numbers of CD4 + and CD8 + T cells infiltrated in tumor were measured by flow cytometry (n \u003d 6). ( B ) The percentage of CD4 + and CD8 + T cells in spleen lymphocytes was analyzed by flow cytometry (n \u003d 6). ( C , D ) Absolute numbers of CD4 + and CD8 + T cells in spleen lymphocytes were detected by flow cytometry ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 25, 2022, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Cellular and molecular gastroenterology and hepatology','journalText':'Cellular and molecular gastroenterology and hepatology 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14925_gr8.jpg','benchSciPubmedId':'34087454'},{'sku':'63-0112-82','imageId':'876498','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14977_41467_2021_23197_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14977_41467_2021_23197_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14977_41467_2021_23197_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14977_41467_2021_23197_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'8','description':'Fig. 1 Generation and characterization of a genetic system for specific targeting of cavity macrophages. a Schematic figure showing CD45-Dre mediates Stop cassette removal from Gata6-iCreER and places CreER directly under Gata6 promoter. After tamoxifen (Tam), Cre-loxP recombination labels cells by tdTomato. b Intersectional genetics marks CD45 + GATA6 + cells as tdTomato + . c Schematic figure showing experimental design using cavity macrophage tracing tool CD45-Dre;Gata6-iCreER ( G6Mo-CreER ). d Flow cytometric analysis of the percentage of tdTomato + cells in macrophages from blood, pleural, and peritoneal cavity with or without Tam. e Immunostaining for tdTomato, CD11b, GATA6, F4/80 on dissociated cells from blood, pleural, or peritoneal cavity. Boxed regions are magnified. f Quantification of the percentage of tdTomato + cells in GATA6 + or F4/80 + macrophages. Data are the mean +- SD; n \u003d 5 mice per group. g Quantification of the percentage of GATA6 + or F4/80 + macrophages in tdTomato + cells. Data are the mean +- SD; n \u003d 5 mice per group. h FACS showing the percentage of tdTomato + cells expressing F4/80. i Whole-mount epifluorescence images and immunostaining for CD45 and tdTomato shows no resident tdTomato + macrophages in visceral organs. Each image is representative of 5 individual biological samples. Scale bars, yellow, 1 mm; white, 100 um. Source data are provided as a Source Data file.','shortDescription':'Fig. 1 Generation and characterization of a genetic system for specific targeting of cavity macrophages. a Schematic figure showing CD45-Dre mediates Stop cassette removal from Gata6-iCreER and places CreER directly under Gata6 promoter. After tamoxifen (Tam), Cre-loxP recombination labels cells by tdTomato. b Intersectional genetics marks CD45 + GATA6 + cells as tdTomato + . c Schematic figure showing expe... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 17, 2021, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14977_41467_2021_23197_Fig1_HTML.jpg','benchSciPubmedId':'34001904'},{'sku':'63-0112-82','imageId':'853091','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0008.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0008.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0008.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0008.jpg?time\u003d20240507','sortOrder':'9','description':'LRRK2 inhibits the fast recycling of receptors internalized through macropinocytosis A-D Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male WT-mLRRK2 BAC transgenic mice were immunostained with anti-mouse CCR5, or CD11b, or MHC II antibody (shown as green signal) and (A) total Rab10 antibody (red signal), or (B) pT73-Rab10 antibody (red signal) as indicated. Representative photomicrographs (from \u003e 20 images analyzed for each condition from n \u003d 3 biologically independent experiments) are shown. (C) Photomicrographs showing the different cargo with respect to Rab10 tubular structures. (D) EEA1 (shown as green signal) with total Rab10 or pT73-Rab10 (shown as red signals) as indicated. Bounding boxes in panels A-D are magnified in \'\'High Mag\'\' panels that show individual vesicles. Scale bars represent 10 and 2 mum for \'\'High Mag\'\' images. E Calculated percentage of vesicles positive with tubular structures ( n \u003e 30 vesicles analyzed from cells from n \u003d 3 biologically independent experiments) are shown. Each dot represents one vesicle, with significance assessed by Mann-Whitney test. ** represents P \u003c 0.01. Error bars represent +- SEM. F BMDM cells were treated with 0.004% DMSO or 100 nM MLi2 prior to live cell surface staining with Alexa-594 conjugated rat anti-mouse CD11b antibody on ice for 10 min. Three wells of cells from each condition were fixed and imaged for initial surface CD11b binding (shown in red as indicated). Six wells of cells from each cond','shortDescription':'LRRK2 inhibits the fast recycling of receptors internalized through macropinocytosis A-D Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male WT-mLRRK2 BAC transgenic mice were immunostained with anti-mouse CCR5, or CD11b, or MHC II antibody (shown as green signal) and (A) total Rab10 antibody (red signal), or (B) pT73-Rab10 antibody (red signal) as indicated. Representative photomicrog... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Oct 15, 2020, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'The EMBO journal','journalText':'The EMBO journal 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0008.jpg','benchSciPubmedId':'32853409'},{'sku':'63-0112-82','imageId':'853081','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0004ev.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0004ev.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0004ev.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0004ev.jpg?time\u003d20240507','sortOrder':'10','description':'EV4 LRRK2 phosphorylates Rab10 on vesicular structures A Raw264.7 cells were transfected with eGFP(N-term)-WT Rab10 (show as green signal) and treated with 0.001% DMSO or 100 nM MLi2 (i.e., ~IC 90 concentration) for 2 h prior to fixation and immunostaining with pT73-Rab10 antibody (shown as red signal). Representative photomicrographs (from \u003e 20 images analyzed for each condition from n \u003d 3 biologically independent experiments) are shown. Scale bars indicate 20 mum. B Raw264.7 cells were transfected with eGFP(N-term)-Rab10 before fixation and analyzed with the pT73-Rab10 antibody. Z-stack images of Rab10 transfected cells were collected using Airyscan microscopy. Orthogonal views of representative images are shown. Red dashed line is the intersection of y,z and yellow dashed line is x,z. Blue box is the x,y plane, red box is the y,z plane, and yellow box is the x,z plane. Scale bar represents 10 mum. C Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male Ntg or WT-mLRRK2 BAC transgenic mice were treated with DMSO or 100 nM MLi2 for 2 h. Representative immunoblots of cells derived from three different animals for each genotype are shown. D, E Relative LRRK2 or pT73-Rab10 levels were calculated as fold changes from Ntg cells (male C57BL/6J) treated with DMSO (0.004% to match DMSO levels with MLi2 treatment). Data are calculated from three independent replicates, with each dot in the column graph represents one independent experiment. Error bars represent +-','shortDescription':'EV4 LRRK2 phosphorylates Rab10 on vesicular structures A Raw264.7 cells were transfected with eGFP(N-term)-WT Rab10 (show as green signal) and treated with 0.001% DMSO or 100 nM MLi2 (i.e., ~IC 90 concentration) for 2 h prior to fixation and immunostaining with pT73-Rab10 antibody (shown as red signal). Representative photomicrographs (from \u003e 20 images analyzed for each condition from n \u003d 3 biologically ind... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Oct 15, 2020, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'The EMBO journal','journalText':'The EMBO journal 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0004ev.jpg','benchSciPubmedId':'32853409'},{'sku':'63-0112-82','imageId':'523927','imageUrl':'https://www.thermofisher.com/antibody/images/650/nihms948434f10-52-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/nihms948434f10-52-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/nihms948434f10-52-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/nihms948434f10-52-20190221105219.jpg?time\u003d20240507','sortOrder':'11','description':'Extended data Figure 6 Premarking in ESCs is required for robust future macrophage enhancer activation a, Photomicrographs of macrophages differentiated from ESCs documented by F4/80 and CD11b staining. Scale bar\u003d50 uM. b , Screenshot of the Tlr1 locus. The blue box corresponds to CRISPR/Cas9 target region. c, d, The putative Tlr1 enhancer mutant sequence diagram and DNA sequence documentation of several of the homozygous mutation clones, used for analysis. e, Screenshot of the Tnfaip3 locus. The blue box corresponds to CRISPR/Cas9 target region. f, g, The putative Tnfaip3 enhancer mutant sequence diagram and DNA sequence documentation of several of the homozygous mutation clones, used for analysis. h, Tlr1 eRNA transcription was tested in wt and mutant clonal cells (#26, #45) of Tnfaip3 enhancer. One representative experiment is plotted (n\u003d2 biological repeats). i, UCSC genome browser shot image of RNA-seq in Tlr1 locus in normal ESCs and clonal mutant ESCs (wt, #10, #14). Primer sets are listed in Supplementary Table 2 .','shortDescription':'Extended data Figure 6 Premarking in ESCs is required for robust future macrophage enhancer activation a, Photomicrographs of macrophages differentiated from ESCs documented by F4/80 and CD11b staining. Scale bar\u003d50 uM. b , Screenshot of the Tlr1 locus. The blue box corresponds to CRISPR/Cas9 target region. c, d, The putative Tlr1 enhancer mutant sequence diagram and DNA sequence documentation o... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Apr 1, 2018, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature','journalText':'Nature 2018 - ','sourceType':'3','benchsci':true,'imageName':'nihms948434f10-52-20190221105219.jpg','benchSciPubmedId':'29670286'},{'sku':'63-0112-82','imageId':'484973','imageUrl':'https://www.thermofisher.com/antibody/images/650/nihms865773f3-1-20190103084817.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/nihms865773f3-1-20190103084817.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/nihms865773f3-1-20190103084817.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/nihms865773f3-1-20190103084817.jpg?time\u003d20240507','sortOrder':'12','description':'Extended Data Figure 3 Wnt ligands produced predominantly by cancer cells drive activation of the Wnt signaling pathway in lung adenocarcinoma a , Schematic representation of the lentivirus vector 46 used to transduce a Kras G12D/+ ; Trp53Delta/Delta ; Rosa26 tdTomato/+ ( KPT ) LUAD cell line, followed by puromycin selection. b , tdTomato and 7TCF::Luciferase signals at baseline (0h) and 48h following treatment with 10 mg/kg/d LGK974 or vehicle. Red arrows indicate two subcutaneous tumours with reduced 7TCF-dependent bioluminescence in response to 48h of LGK974 treatment. c , Suppression of 7TCF-driven bioluminescence by LGK974 relative to tdTomato signal in mice harboring subcutaneous transplants of the KPT LUAD cell line stably expressing the 7TCF::Luciferase-PGK-Puro lentivirus. N \u003d 6 tumours, 3 mice per group. Representative data from two independent experiments. Student\u0027s two-sided t -test; error bars: +-SD. d , Haematoxylin-eosin (HE) or beta-catenin staining in KP adenomas or in adenocarcinomas. Scale bars: 100 mum. e , Immunofluorescence for beta-catenin (red) and Porcupine (green) in a Kras G12D/+ ; Trp53Delta/Delta ( KP ) lung adenocarcinoma (LUAD). Scale bar: 100 mum. f , In situ hybridization (ISH) for Axin2 mRNA (purple, arrowheads) in KP lung adenomas or adenocarcinomas. Scale bar: 50 mum. g , Immunofluorescence for Porcupine (green, white arrowheads) and ISH for Axin2 (red, yellow arrowheads) in KP LUAD. Scale bar: 10 mum. h , Immunofluorescence for Porcupine (','shortDescription':'Extended Data Figure 3 Wnt ligands produced predominantly by cancer cells drive activation of the Wnt signaling pathway in lung adenocarcinoma a , Schematic representation of the lentivirus vector 46 used to transduce a Kras G12D/+ ; Trp53Delta/Delta ; Rosa26 tdTomato/+ ( KPT ) LUAD cell line, followed by puromycin selection. b , tdTomato and 7TCF::Luciferase signals at baseline (0h) and 48h following treat... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 18, 2017, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature','journalText':'Nature 2017 - ','sourceType':'3','benchsci':true,'imageName':'nihms865773f3-1-20190103084817.jpg','benchSciPubmedId':'28489818'},{'sku':'63-0112-82','imageId':'317201','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_3278_srep36659-f7.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_3278_srep36659-f7.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_3278_srep36659-f7.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_3278_srep36659-f7.jpg?time\u003d20240507','sortOrder':'13','description':'Figure 7 Macrophages form a perfused vascular network in tumors. ( A-D ) Images represent individual laser channels of a 38 mum Z-stack representing an A375 human melanoma subcutaneous tumor in an athymic mouse. Tumor cells were injected in the absence of matrigel and tumor perfusion was assessed by administering a 3 KD rhodamine dextran by tail vein injection prior to tumor removal. Tumor was harvested, fixed and immunostained with anti-mouse antibodies as follows: ( A ) Hoechst nuclear stain (blue), ( B ) CD11b (purple), ( C ) CD31 (green) and ( D ) iv 3 KD rhodamine dextran (red). ( E ) Merged image comprised of the four fluorescent channels presented in ( A-D ). Note that most of the perfused structures are strongly positive for CD11b. ( F-H ) Magnified view of individual laser channels in the area highlighted by the white box in ( A-D ) shows that the intravenously injected dye localizes to the CD11b + tubular structure (blue arrow) which is not CD31 + . ( I ) Imaris 3D cross sectional rendering of the CD11b + vessels depicted by the dashed white line in image ( E ) shows the dye to be within the lumen of the CD11b + channel. ( J-N ) CD11b + tubes are connected to multinucleated, tuft-like structures which express CD31, CD11b and are perfused by systemically injected dye. ( J ) Low power view of a rosette-like structure within the tumor associated macrophage VM network. Circled area shows an Imaris rendered tumor tuft ( K-N ) that is connected to the predominately CD11b','shortDescription':'Figure 7 Macrophages form a perfused vascular network in tumors. ( A-D ) Images represent individual laser channels of a 38 mum Z-stack representing an A375 human melanoma subcutaneous tumor in an athymic mouse. Tumor cells were injected in the absence of matrigel and tumor perfusion was assessed by administering a 3 KD rhodamine dextran by tail vein injection prior to tumor removal. Tumor was harvested, fi... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)','title':'CD11b Antibody (63-0112-82) in ICC/IF, IHC','appAbv':'IHC','dateTime':'Nov 11, 2016, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2016 - ','sourceType':'3','benchsci':true,'imageName':'tfs_3278_srep36659-f7.jpg','benchSciPubmedId':'27834402'},{'sku':'63-0112-82','imageId':'332782','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27158241_IF_12_gr4_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27158241_IF_12_gr4_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27158241_IF_12_gr4_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27158241_IF_12_gr4_20180524.jpg?time\u003d20240507','sortOrder':'14','description':'Fig. 4 Ginsenoside Rd reduces M1 macrophage in situ infiltration in IRI tissue detected by immunofluorescence staining. (A-C)Red and green fluorescence indicates CD11b + , iNOS + /tumor necrosis factor-alpha + /interleukin-12 + macrophages, respectively. Blue fluorescence indicates total nuclei in renal tissue. Original magnification: 400x. (D-F) Corresponding percentage of M1 macrophage among all kidney cells. (Eight randomly selected fields/slice, n \u003d 6 mice for each group, values represent mean +- SD in the histograms, * p \u003c 0.05 vs vehicle.)','shortDescription':'Fig. 4 Ginsenoside Rd reduces M1 macrophage in situ infiltration in IRI tissue detected by immunofluorescence staining. (A-C)Red and green fluorescence indicates CD11b + , iNOS + /tumor necrosis factor-alpha + /interleukin-12 + macrophages, respectively. Blue fluorescence indicates total nuclei in renal tissue. Original magnification: 400x. (D-F) Corresponding percentage of M1 macrophage among all kidney ce... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Apr 1, 2016, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of ginseng research','journalText':'Journal of ginseng research 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27158241_IF_12_gr4_20180524.jpg','benchSciPubmedId':'27158241'},{'sku':'63-0112-82','imageId':'329366','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646012_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646012_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27066505_IF_12_JIR2016-4325646012_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27066505_IF_12_JIR2016-4325646012_20180524.jpg?time\u003d20240507','sortOrder':'15','description':'Figure 12 Fibronectin and type I collagen (collagen I) in the cells in the fragments of mouse splenic granulomas. The scale bars are ((a, right panel), (c, right panel)) 20 mu m each and ((a)-(e)) 10 mu m each. ((b), (d)-(e)) Colocalization of the markers on confocal images of cells (yellow signal). Nuclei stained by DAPI (blue signal). ((a), (c)) Immunochemical localization of (a) fibronectin and (c) type I collagen in granuloma cells and control mouse peritoneal macrophages (Mph) and splenic fibroblasts (Fibr). The brown color indicates the presence of the markers in these cells. The black arrows indicate fibroblasts; the other cells are macrophages, dendritic cells, and lymphocytes. (b) Confocal immunofluorescent localization of fibronectin (green signal) and CD11b (red signal) in granuloma macrophages. In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. A macrophage with BCG-mycobacteria reproducing in it is indicated by the white arrows on the fluorescent images and the black arrow on the ZN image. (d) Confocal immunofluorescent localization of type I collagen (green signal) and CD11b (red signal) in a granuloma macrophage with BCG-mycobacteria. In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. (e) Confocal immunofluorescent localization of fibronectin (green signal), type I collagen (green signal), and filamentous actin (red signal) i','shortDescription':'Figure 12 Fibronectin and type I collagen (collagen I) in the cells in the fragments of mouse splenic granulomas. The scale bars are ((a, right panel), (c, right panel)) 20 mu m each and ((a)-(e)) 10 mu m each. ((b), (d)-(e)) Colocalization of the markers on confocal images of cells (yellow signal). Nuclei stained by DAPI (blue signal). ((a), (c)) Immunochemical localization of (a) fibronectin and (c) type ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 8, 2016, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of immunology research','journalText':'Journal of immunology research 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27066505_IF_12_JIR2016-4325646012_20180524.jpg','benchSciPubmedId':'27066505'},{'sku':'63-0112-82','imageId':'317643','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646007_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646007_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27066505_IF_12_JIR2016-4325646007_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27066505_IF_12_JIR2016-4325646007_20180524.jpg?time\u003d20240507','sortOrder':'16','description':'Figure 7 ((a)-(c)) Bacterial lipid- and glycolipid-presenting molecule CD1d in the cells in the fragments of mouse splenic granulomas. The scale bars are ((a, central right panel), (b)-(d)) 10 mu m each and (a, other panels) 20 mu m each. ((b)-(c)) Colocalization of the markers on the confocal images of cells (yellow signal). ((b)-(d)) Nuclei stained by DAPI (blue signal). (a) Immunochemical localization of CD1d in granuloma cells, control mouse peritoneal macrophages (Mph), and fibroblasts (Fibr). The brown color indicates the presence of CD1d in these cells. ((b)-(c)) Confocal immunofluorescent localization of CD1d (green signal) and IL-1 alpha (red signal) in granuloma macrophages and fibroblasts. (b) CD1d- and IL-1 alpha -producing fibroblasts are indicated by the white arrows on the fluorescent images and the black arrows on the 3D image. (c) In the right panel, the same fragment as in the left panel restained for acid-fast BCG-mycobacteria by the ZN method. A CD1d- and IL-1 alpha -producing macrophage with BCG-mycobacteria reproducing in it is indicated by the white arrow on the fluorescent image and the black arrow on the ZN image. (d) Confocal immunofluorescent localization of CD1d (red signal) in the cells in the cultures of bone marrow macrophages following infection with the BCG vaccine in vitro and after culture for several hours. The macrophage with CD1d in it is indicated by the red arrow, and a megakaryocyte without the antigen is indicated by the white arrow.','shortDescription':'Figure 7 ((a)-(c)) Bacterial lipid- and glycolipid-presenting molecule CD1d in the cells in the fragments of mouse splenic granulomas. The scale bars are ((a, central right panel), (b)-(d)) 10 mu m each and (a, other panels) 20 mu m each. ((b)-(c)) Colocalization of the markers on the confocal images of cells (yellow signal). ((b)-(d)) Nuclei stained by DAPI (blue signal). (a) Immunochemical localization of... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 8, 2016, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of immunology research','journalText':'Journal of immunology research 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27066505_IF_12_JIR2016-4325646007_20180524.jpg','benchSciPubmedId':'27066505'},{'sku':'63-0112-82','imageId':'319085','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646006_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646006_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27066505_IF_12_JIR2016-4325646006_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27066505_IF_12_JIR2016-4325646006_20180524.jpg?time\u003d20240507','sortOrder':'17','description':'Figure 6 ((a)-(c)) The cells with IFN gamma in the fragments of mouse splenic granulomas. The scale bars are 10 mu m each. ((a), (c)) Colocalization of the markers on the confocal images of cells (yellow signal). ((a), (c)-(d)) Nuclei stained by DAPI (blue signal). (a) Confocal immunofluorescent localization of IFN gamma (green signal) and CD80 (red signal) in granuloma macrophages (Mph) and a fibroblast at the center of the images. In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. An IFN gamma -producing macrophage with BCG-mycobacteria reproducing in it is indicated by the white arrows on the fluorescent images and the black arrow on the ZN image. (b) Immunochemical localization of IFN gamma in granuloma cells and control mouse peritoneal macrophages. The brown color indicates the presence of IFN gamma in these cells. (c) Confocal immunofluorescent localization of IFN gamma (green signal) and CD11b (red signal) in granuloma macrophages, with a multinucleate Langhans giant cell indicated by the white arrows in the three lower panels. (d) Bone marrow macrophages following infection with the BCG vaccine in vitro and after culture for several hours not stained by the IFN gamma - (green signal) and IL-1 alpha -specific antibodies (red signal) on the confocal fluorescent images. Abbreviations as in Figure 1 .','shortDescription':'Figure 6 ((a)-(c)) The cells with IFN gamma in the fragments of mouse splenic granulomas. The scale bars are 10 mu m each. ((a), (c)) Colocalization of the markers on the confocal images of cells (yellow signal). ((a), (c)-(d)) Nuclei stained by DAPI (blue signal). (a) Confocal immunofluorescent localization of IFN gamma (green signal) and CD80 (red signal) in granuloma macrophages (Mph) and a fibroblast at... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 8, 2016, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of immunology research','journalText':'Journal of immunology research 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27066505_IF_12_JIR2016-4325646006_20180524.jpg','benchSciPubmedId':'27066505'},{'sku':'63-0112-82','imageId':'329335','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646014_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646014_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27066505_IF_12_JIR2016-4325646014_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27066505_IF_12_JIR2016-4325646014_20180524.jpg?time\u003d20240507','sortOrder':'18','description':'Figure 14 The S100 proteins in the cells in the fragments of mouse splenic granulomas and control mouse peritoneal macrophages (Mph). The scale bars are 10 mu m each. In the left panel, confocal immunofluorescent localization of S100 proteins (green signal) and CD11b (red signal). Colocalization of the markers on confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the central and right panels, immunochemical localization of S100 proteins in the cells. The brown color indicates the presence of the markers in these cells. The black and red arrows indicate macrophages and a dendritic cell, respectively, with increased production of S100 proteins. Abbreviations as in Figure 1 .','shortDescription':'Figure 14 The S100 proteins in the cells in the fragments of mouse splenic granulomas and control mouse peritoneal macrophages (Mph). The scale bars are 10 mu m each. In the left panel, confocal immunofluorescent localization of S100 proteins (green signal) and CD11b (red signal). Colocalization of the markers on confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the cent... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 8, 2016, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of immunology research','journalText':'Journal of immunology research 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27066505_IF_12_JIR2016-4325646014_20180524.jpg','benchSciPubmedId':'27066505'},{'sku':'63-0112-82','imageId':'329352','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646013_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646013_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27066505_IF_12_JIR2016-4325646013_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27066505_IF_12_JIR2016-4325646013_20180524.jpg?time\u003d20240507','sortOrder':'19','description':'Figure 13 Confocal immunofluorescent localization of vimentin (green signal) and CD11b (red signal) in the cells in a fragment of a mouse splenic granuloma. The scale bars are 10 mu m each. Colocalization of the markers on the confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. Macrophages with BCG-mycobacteria reproducing in them are indicated by the white arrows on the fluorescent images and the black arrows on the ZN image. Abbreviations as in Figure 1 .','shortDescription':'Figure 13 Confocal immunofluorescent localization of vimentin (green signal) and CD11b (red signal) in the cells in a fragment of a mouse splenic granuloma. The scale bars are 10 mu m each. Colocalization of the markers on the confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacter... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 8, 2016, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of immunology research','journalText':'Journal of immunology research 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27066505_IF_12_JIR2016-4325646013_20180524.jpg','benchSciPubmedId':'27066505'},{'sku':'63-0112-82','imageId':'362253','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_26656646_IF_12_nihms776849f2_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_26656646_IF_12_nihms776849f2_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_26656646_IF_12_nihms776849f2_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_26656646_IF_12_nihms776849f2_20180524.jpg?time\u003d20240507','sortOrder':'20','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jan 1, 2016, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature neuroscience','journalText':'Nature neuroscience 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_26656646_IF_12_nihms776849f2_20180524.jpg','benchSciPubmedId':'26656646'},{'sku':'63-0112-82','imageId':'880473','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14946_urn-x-wiley-00219541-media-jcp25239-jcp25239-fig-0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14946_urn-x-wiley-00219541-media-jcp25239-jcp25239-fig-0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14946_urn-x-wiley-00219541-media-jcp25239-jcp25239-fig-0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14946_urn-x-wiley-00219541-media-jcp25239-jcp25239-fig-0003.jpg?time\u003d20240507','sortOrder':'21','description':'FLCs are different from macrophages. (A) Cell morphology of mouse FLCs at passage 2 (right panel) and macrophage cell line Raw 264.7 (left panel). (B) FLCs at passage 1 and Raw264.7 were stained with either CD11b or CD11c or CD14 antibody. Scale bars, 50 mum.','shortDescription':'FLCs are different from macrophages. (A) Cell morphology of mouse FLCs at passage 2 (right panel) and macrophage cell line Raw 264.7 (left panel). (B) FLCs at passage 1 and Raw264.7 were stained with either CD11b or CD11c or CD14 antibody. Scale bars, 50 mum.','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jun 1, 2016, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of cellular physiology','journalText':'Journal of cellular physiology 2016 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14946_urn-x-wiley-00219541-media-jcp25239-jcp25239-fig-0003.jpg','benchSciPubmedId':'26529564'},{'sku':'63-0112-82','imageId':'335213','imageUrl':'https://www.thermofisher.com/antibody/images/650/14011282-26275291-CD11b-pone0133298g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/14011282-26275291-CD11b-pone0133298g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/14011282-26275291-CD11b-pone0133298g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/14011282-26275291-CD11b-pone0133298g002.jpg?time\u003d20240507','sortOrder':'22','description':'Fig 2 Virulent Yersinia becomes partially embedded within the macrophage plasma membrane. (A) RAW macrophages were infected with YPIII/pYV for 100 minutes and analyzed by scanning electron microscopy (SEM). Shown is a representative image depicting a single macrophage infected with several YPIII/pYV bacteria. Shown in the enlargements are bacteria partially enveloped within host membrane. The bacterium in panel b is indicated by an arrow. Shown below is a false coloring rendering highlighting the demarcation of the host cell (blue) and YPIII/pYV bacteria (red). (B) Macrophages were infected with YPIII/pYV for 100 minutes and then stained for the plasma membrane marker (CD11b), the early endosomal marker (EEA1), and a lysosomal marker (LAMP1) and analyzed by fluorescent microscopy. The arrow in the enlarged image of the CD11b-stained cells indicates a bacterium closely associated with host cell membrane; 62% of macrophage-associated bacteria (N \u003d 56) were similarly observed. Shown in the right panels of the EEA1- and LAMP1-stained cells is the percentage of bacteria-containing endosomes that stained for either EEA1 or LAMP1 (N \u003d 111 and 52, respectively). The arrow in the enlarged image indicates a bacterium distinct from an EEA1-staining compartment. The data shown is representative of 3 independent experiments with similar results using both RAW macrophages and murine peritoneal macrophages.','shortDescription':'Fig 2 Virulent Yersinia becomes partially embedded within the macrophage plasma membrane. (A) RAW macrophages were infected with YPIII/pYV for 100 minutes and analyzed by scanning electron microscopy (SEM). Shown is a representative image depicting a single macrophage infected with several YPIII/pYV bacteria. Shown in the enlargements are bacteria partially enveloped within host membrane. The bacterium in p... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 10, 2016, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2016 - ','sourceType':'3','benchsci':true,'imageName':'14011282-26275291-CD11b-pone0133298g002.jpg','benchSciPubmedId':'26275291'},{'sku':'63-0112-82','imageId':'362241','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_26275291_IF_13_pone0133298s003_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_26275291_IF_13_pone0133298s003_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_26275291_IF_13_pone0133298s003_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_26275291_IF_13_pone0133298s003_20180524.jpg?time\u003d20240507','sortOrder':'23','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 10, 2016, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_26275291_IF_13_pone0133298s003_20180524.jpg','benchSciPubmedId':'26275291'},{'sku':'63-0112-82','imageId':'358608','imageUrl':'https://www.thermofisher.com/antibody/images/650/16011282-26080408-CD11b-pnas1424355112sfig04.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/16011282-26080408-CD11b-pnas1424355112sfig04.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/16011282-26080408-CD11b-pnas1424355112sfig04.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/16011282-26080408-CD11b-pnas1424355112sfig04.jpg?time\u003d20240507','sortOrder':'24','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Jun 30, 2015, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Proceedings of the National Academy of Sciences of the United States of America','journalText':'Proceedings of the National Academy of Sciences of the United States of America 2015 - ','sourceType':'3','benchsci':true,'imageName':'16011282-26080408-CD11b-pnas1424355112sfig04.jpg','benchSciPubmedId':'26080408'},{'sku':'63-0112-82','imageId':'326219','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_24463523_IF_12_nihms543080f3_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_24463523_IF_12_nihms543080f3_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_24463523_IF_12_nihms543080f3_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_24463523_IF_12_nihms543080f3_20180524.jpg?time\u003d20240507','sortOrder':'25','description':'Figure 3 AM-MyD88 in inflammatory signaling ( a-c ) Synchronized (Syn) and non-synchronized (Non) Ca 2+ spikes (arrows) and oscillations in wild type (WT, n \u003d 12), CD11cMyD88 -/- (My -/- , n \u003d 5) and CD11cCx43 -/- (Cx -/- , n \u003d 5) mice. ( d ) Alveoli (green), and Ly6G + (red) and CD11b + neutrophils (blue) 24 h after LPS. Scale bar, 30 mum. n \u003d 4 ( e ) Responses are 24 h after treatments. LPS WT: n \u003d 9, others: n \u003d 4. Bars, mean+-SEM. *P\u003c0.05.','shortDescription':'Figure 3 AM-MyD88 in inflammatory signaling ( a-c ) Synchronized (Syn) and non-synchronized (Non) Ca 2+ spikes (arrows) and oscillations in wild type (WT, n \u003d 12), CD11cMyD88 -/- (My -/- , n \u003d 5) and CD11cCx43 -/- (Cx -/- , n \u003d 5) mice. ( d ) Alveoli (green), and Ly6G + (red) and CD11b + neutrophils (blue) 24 h after LPS. Scale bar, 30 mum. n \u003d 4 ( e ) Responses are 24 h after treatments. LPS WT: n \u003d 9, oth... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Feb 27, 2014, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Nature','journalText':'Nature 2014 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_24463523_IF_12_nihms543080f3_20180524.jpg','benchSciPubmedId':'24463523'},{'sku':'63-0112-82','imageId':'362105','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_24446236_IF_12_nihms562085f7_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_24446236_IF_12_nihms562085f7_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_24446236_IF_12_nihms562085f7_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_24446236_IF_12_nihms562085f7_20180524.jpg?time\u003d20240507','sortOrder':'26','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 1, 2014, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Stem cells (Dayton, Ohio)','journalText':'Stem cells (Dayton, Ohio) 2014 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_24446236_IF_12_nihms562085f7_20180524.jpg','benchSciPubmedId':'24446236'},{'sku':'63-0112-82','imageId':'320019','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_22361049_IF_13_1742-2094-9-39-5_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_22361049_IF_13_1742-2094-9-39-5_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_22361049_IF_13_1742-2094-9-39-5_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_22361049_IF_13_1742-2094-9-39-5_20180524.jpg?time\u003d20240507','sortOrder':'27','description':'Figure 5 Microglial activation in whole-mount retina and microglial migration in an in vitro model . ( A ) Microglia in wild-type (WT) retina have a different shape from those in the trif -/- group as shown by CD11b immunostaining at 7 and 14 days post-crush (7dPC and 14dPC). Scale bar \u003d 20 mum. ( B ) Estimated numbers of microglia/mm 2 in whole-mount retinas were different between different time-course groups (* P \u003c 0.05 increase relative to sham and 14dPC group). ( C ) Estimated number per mm 2 of microglia with an amoeboid shape in whole-mount retinas were different between wild-type (WT and trif -/- groups at 7 and 14 days post-crush (7dPC and 14dPC). * P \u003c 0.01 increase relative to WT group in 7dPC and 14dPC. ( D ) Outline of whole-mount retina. Square frames (red) were counted using double-blinded quantification. ( E ) In a transwell system, microglia and retinal ganglion cells (RGCs) were cultured together (as shown in drawing). Microglia became activated and migrated to the other side of the membrane when stimulated by injured RGCs in the lower well. Stained with cresyl violet acetate. Scale bar \u003d 20 mum.','shortDescription':'Figure 5 Microglial activation in whole-mount retina and microglial migration in an in vitro model . ( A ) Microglia in wild-type (WT) retina have a different shape from those in the trif -/- group as shown by CD11b immunostaining at 7 and 14 days post-crush (7dPC and 14dPC). Scale bar \u003d 20 mum. ( B ) Estimated numbers of microglia/mm 2 in whole-mount retinas were different between different time-course gro... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunocytochemistry (ICC/IF)','title':'CD11b Antibody (63-0112-82) in ICC/IF','appAbv':'ICC/IF','dateTime':'Feb 24, 2012, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of neuroinflammation','journalText':'Journal of neuroinflammation 2012 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_22361049_IF_13_1742-2094-9-39-5_20180524.jpg','benchSciPubmedId':'22361049'},{'sku':'63-0112-82','imageId':'878811','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14904_biology-10-00472-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14904_biology-10-00472-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14904_biology-10-00472-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14904_biology-10-00472-g005.jpg?time\u003d20240507','sortOrder':'28','description':'Figure 5 Expression of IL-16 by CNS resident cells in EAE mice. Spinal cord and brain tissues were harvested from EAE and PBS control mice and stained with IL-16 (green) together with NeuN, GFAP or CD11b (all red). Representative images of spinal cord ( A - C ) and hippocampus ( D ) tissues are shown. Dotted white lines indicate tissue section edges. White arrows indicate areas of colocalisation. Percentage of double-positive cells in each group within either the spinal cord ( A - C ) or hippocampus ( E - G ) and cerebellum ( H - J ) was quantified with results expressed as the mean +- SEM. N \u003d 5. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc tests. * p \u003c 0.05, ** p \u003c 0.01, *** p \u003c 0.001 versus PBS or comparison groups are indicated by the bar lines.','shortDescription':'Figure 5 Expression of IL-16 by CNS resident cells in EAE mice. Spinal cord and brain tissues were harvested from EAE and PBS control mice and stained with IL-16 (green) together with NeuN, GFAP or CD11b (all red). Representative images of spinal cord ( A - C ) and hippocampus ( D ) tissues are shown. Dotted white lines indicate tissue section edges. White arrows indicate areas of colocalisation. Percentage... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))','title':'CD11b Antibody (63-0112-82) in IHC (F)','appAbv':'IHC (F)','dateTime':'May 27, 2021, 4:00:00 AM','appName':'Immunohistochemistry (Frozen)','type':'Published Figures','journalTitle':'Biology','journalText':'Biology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14904_biology-10-00472-g005.jpg','benchSciPubmedId':'34071825'},{'sku':'63-0112-82','imageId':'841453','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12625_iovs-62-4-22-f008.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12625_iovs-62-4-22-f008.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12625_iovs-62-4-22-f008.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12625_iovs-62-4-22-f008.jpg?time\u003d20240507','sortOrder':'29','description':'Figure 8. Both CD and HFD cause increased retinal immune activation, as measured by CD11b ( green ) and Iba1 ( red ). Representative retinal immunofluorescence microscopy results, with ( left ) and without ( right ) DAPI staining ( blue ), showing retinal layers of the ( A ) CL + CON group, the ( B ) CL + HFD group, the ( C ) CD + CON group, and the ( D ) CD + HFD group show increased retinal expression of CD11b in CL + HFD, CD + CON, and CD + HFD groups and increased retinal expression of Iba1 in the CD + CON and CD + HFD groups. Measured fluorescence by retinal layer, showing no difference in ( E ) CD11b staining in the OPL (1-way ANOVA, F(3, 17) \u003d 1.23, P \u003d 0.33) but increased Iba1 staining in the OPL (1-way ANOVA, F(3, 17) \u003d 6.25, P \u003d 0.0047) in the CD + HFD group. ( F ) In the INL, there was also no difference in CD11b expression (1-way ANOVA, F(3, 17) \u003d 2.52, P \u003d 0.092), but Iba1 expression was increased (1-way ANOVA, F(3, 17) \u003d 5.36, P \u003d 0.022) in the CD + CON and CD + HFD groups. In the IPL, ( G ) CD11b increased (1-way ANOVA, F(3, 17) \u003d 8.06, P \u003d 0.0015) in both HFD groups while Iba1 increased (1-way ANOVA, F(3, 17) \u003d 15.87, P \u003c 0.0001) in both CD groups. Likewise, in the GCL, ( H ) CD11b was increased (1-way ANOVA, F(3, 17) \u003d 4.34, P \u003d 0.019) in the CL + HFD group and Iba1 was increased (1-way ANOVA, F(3, 17) \u003d 3.89, P \u003d 0.023) in both CD groups. ( I ) Drawing representing the retinal layers. Images from the CL + CON group ( n \u003d 5 mice), CL + HFD group ( n \u003d 4 mice)','shortDescription':'Figure 8. Both CD and HFD cause increased retinal immune activation, as measured by CD11b ( green ) and Iba1 ( red ). Representative retinal immunofluorescence microscopy results, with ( left ) and without ( right ) DAPI staining ( blue ), showing retinal layers of the ( A ) CL + CON group, the ( B ) CL + HFD group, the ( C ) CD + CON group, and the ( D ) CD + HFD group show increased retinal expression of ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))','title':'CD11b Antibody (63-0112-82) in IHC (F)','appAbv':'IHC (F)','dateTime':'Apr 1, 2021, 4:00:00 AM','appName':'Immunohistochemistry (Frozen)','type':'Published Figures','journalTitle':'Investigative ophthalmology \u0026 visual science','journalText':'Investigative ophthalmology \u0026 visual science 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12625_iovs-62-4-22-f008.jpg','benchSciPubmedId':'33861321'},{'sku':'63-0112-82','imageId':'324668','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_1457_1472-6750-9-1-3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_1457_1472-6750-9-1-3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_1457_1472-6750-9-1-3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_1457_1472-6750-9-1-3.jpg?time\u003d20240507','sortOrder':'30','description':'Figure 3 Survival of BMSC genetically modified with multiple reporter genes following implantation in the central nervous system of syngeneic immunocompetent mice . (A) Histogram overlay showing a representative flow cytometric analysis of eGFP expression by BMSC expressing the luciferase-, eGFP- and puromycin resistance genes (BMSC-Luc/eGFP/Pac, filled histogram). Parental BMSC were used as negative control (open histogram). (B) In vitro luminescence assay on parental BMSC and on clonal BMSC-Luc/eGFP/Pac. (C) In vivo real time bioluminescence imaging (BLI) of clonal BMSC-Luc/eGFP/Pac grafts in the central nervous system (CNS) of syngeneic immunocompetent mice at week 2 post-implantation. (D) In vivo real time BLI of clonal BMSC-Luc/eGFP/Pac intramuscular grafts in syngeneic immunocompetent mice at week 2 post-injection. (E) Representative histological analysis of clonal BMSC-Luc/eGFP/Pac implants in the CNS of syngeneic immunocompetent mice at week 2 post-implantation. Left picture: haematoxylin-eosin (HE) staining showing general appearance of the cell implantation site. Middle picture: direct eGFP-fluorescence indicating the BMSC-Luc/eGFP/Pac origin of the observed cell implant. Right picture: CD11b staining indicating a limited number of microglia surrounding the observed cell graft. All slides were examined using a conventional bright field microscope and digital pictures were taken under magnification as indicated by the scale bars.','shortDescription':'Figure 3 Survival of BMSC genetically modified with multiple reporter genes following implantation in the central nervous system of syngeneic immunocompetent mice . (A) Histogram overlay showing a representative flow cytometric analysis of eGFP expression by BMSC expressing the luciferase-, eGFP- and puromycin resistance genes (BMSC-Luc/eGFP/Pac, filled histogram). Parental BMSC were used as negative contro... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))','title':'CD11b Antibody (63-0112-82) in IHC, IHC (F)','appAbv':'IHC','dateTime':'Jan 7, 2009, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'BMC biotechnology','journalText':'BMC biotechnology 2009 - ','sourceType':'3','benchsci':true,'imageName':'tfs_1457_1472-6750-9-1-3.jpg','benchSciPubmedId':'19128466'},{'sku':'63-0112-82','imageId':'841468','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12626_JIR-14-1789-g0004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12626_JIR-14-1789-g0004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12626_JIR-14-1789-g0004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12626_JIR-14-1789-g0004.jpg?time\u003d20240507','sortOrder':'31','description':'Figure 4 Rb1 mitigates inflammation in Ang II-infused mice. C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 6.25 mg/kg (Rb1-L), 25 mg/kg (Rb1-M) and 100 mg/kg (Rb1-H). ( A ) Plasma was isolated and analyze by ELISA for the level of IL-1 beta, IL-6 and TNF (n\u003d6-8 per group). ( B ) Immunohistochemistry (IHC) was performed to examine the presence of CD68 + cells (in red) in the heart. DAPI (in blue) was counterstained to help visualize the nuclei. Scale bar, 50 mum. ( C ) The number of CD68 + cells in the heart was quantified (n\u003d5 per group). ( D ) IHC was performed to reveal the presence of CD11b + cells (in red) in the heart. DAPI (in blue) counterstaining was performed to visualize the nuclei. Scale bar, 50 mum. ( E ) The number of CD11b + cells in the heart was quantified (n\u003d5 per group). ( F) . C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 100 mg/kg (Rb1). The cardiac expression of Il1b, II6 , and Tnf was analyzed by real-time qPCR (n\u003d6-7 per group). 18S rRNA was included as the internal control. Relative fold change was plotted against that from the sham controls. Data were expressed as mean+-S.E.M. * P\u003c0.05, ** P\u003c0.01, *** P\u003c0.001.','shortDescription':'Figure 4 Rb1 mitigates inflammation in Ang II-infused mice. C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 6.25 mg/kg (Rb1-L), 25 mg/kg (Rb1-M) and 100 mg/kg (Rb1-H). ( A ) Plasma was isolated and analyze by ELISA for the level of IL-1 beta, IL-6 and TNF (n\u003d6-8 per group). ( ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Apr 23, 2022, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of inflammation research','journalText':'Journal of inflammation research 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12626_JIR-14-1789-g0004.jpg','benchSciPubmedId':'33981156'},{'sku':'63-0112-82','imageId':'841438','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12624_ijms-22-03452-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12624_ijms-22-03452-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12624_ijms-22-03452-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12624_ijms-22-03452-g003.jpg?time\u003d20240507','sortOrder':'32','description':'Figure 3 CD11b- and TLR-2-activated alveolar macrophages with an increased expression of the NF- kappa B p50 and p65 subunits in them are detected on the histological sections obtained from the lung tissues distant from tuberculomas (d), but not from tuberculoma walls (t) for TB patients 24-29. ( A ) The number of alveolar macrophages with the markers expressed as the percentage of the total number of the TB patients\u0027\u0027 alveolar macrophages analyzed. The expression of the markers is shown as in Figure 2 A. Red arrows indicate a trend in the alteration of the number of the markers-positive alveolar macrophages between various lung lesions of the same TB patients. ( B - D ) Representative confocal fluorescent images of cells stained by antibodies to human ( B ) CD14 (green signal) and TLR-2 (red signal), ( C ) NF- kappa B p50 (red signal), ( D ) NF- kappa B p65 (green signal), and CD11b (red signal) demonstrate colocalization of some markers (yellow signal). Nuclei are stained by DAPI (blue signal). Close-ups of the parts of these images with alveolar macrophages and other cells, with or without the examined markers in them, are shown in the corners. The scale bars are 50 um for the histological sections and 10 um for the enlarged views of them. ( C , D ) The NF- kappa B subunits localize in the cytoplasm, but not in the nuclei of alveolar macrophages. White arrows point to alveolar macrophages used to create the profile images. Red arrows identify the areas for the construction','shortDescription':'Figure 3 CD11b- and TLR-2-activated alveolar macrophages with an increased expression of the NF- kappa B p50 and p65 subunits in them are detected on the histological sections obtained from the lung tissues distant from tuberculomas (d), but not from tuberculoma walls (t) for TB patients 24-29. ( A ) The number of alveolar macrophages with the markers expressed as the percentage of the total number of the T... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Mar 26, 2021, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'International journal of molecular sciences','journalText':'International journal of molecular sciences 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12624_ijms-22-03452-g003.jpg','benchSciPubmedId':'33810600'},{'sku':'63-0112-82','imageId':'853361','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9857_41419_2020_3223_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9857_41419_2020_3223_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9857_41419_2020_3223_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9857_41419_2020_3223_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'33','description':'Fig. 3 ZD55-IL-24 inhibits melanoma growth in B16-bearing immunocompetent mouse model mainly through the indirect pathway of inducing systemic antitumor immunity. A , B Changes of the immune-related cytokines in tumors treated with ZD55-IL-24. A Heat-map of the down-regulated immunosuppressive cytokines in Fig. 2 C . B Heat-map of the up-regulated immunostimulatory cytokines in Fig. 2 D . C - J Immunohistochemical analysis of immune cell infiltration in tumors, as well as recruitment and activation in spleens. Tumors and spleens resected from B16-bearing C57BL/6 mice receiving PBS or ZD55-IL-24 treatment indicated in Fig. 1 A were analyzed 2 days after the last injection by immunohistochemical staining. C Shown are representative images of tumor sections immunostained for CD8 (red, left panel), CD11b and Ly-6G (yellow, middle panel), and NK1.1 (red, right panel). D Quantification of the CD8 + cells in C ( n \u003d 11). E Quantification of the CD11b + Ly-6G + Ly-6C low neutrophils in C ( n \u003d 3). f Quantification of the NK1.1 + cells in c ( n \u003d 6). G Quantification of the CD8 + cells in H ( n \u003d 9). H Shown are representative images of spleen sections immunostained for CD8 (red, left panel), CD11b and Ly-6G (yellow, middle panel), and NK1.1 (red, right panel). I Quantification of the CD11b + Ly-6G + Ly-6C low neutrophils in H ( n \u003d 3). j Quantification of NK1.1 + cells in h ( n \u003d 9). Nuclei is counterstained with DAPI (blue). Data are mean +- SD. Scale bars, 300 um. Shown is one of t','shortDescription':'Fig. 3 ZD55-IL-24 inhibits melanoma growth in B16-bearing immunocompetent mouse model mainly through the indirect pathway of inducing systemic antitumor immunity. A , B Changes of the immune-related cytokines in tumors treated with ZD55-IL-24. A Heat-map of the down-regulated immunosuppressive cytokines in Fig. 2 C . B Heat-map of the up-regulated immunostimulatory cytokines in Fig. 2 D . C - J Immunohistoc... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)','title':'CD11b Antibody (63-0112-82) in IHC, Flow','appAbv':'Flow','dateTime':'Nov 30, 2020, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cell death \u0026 disease','journalText':'Cell death \u0026 disease 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9857_41419_2020_3223_Fig3_HTML.jpg','benchSciPubmedId':'33257647'},{'sku':'63-0112-82','imageId':'853322','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9856_cancers-12-03457-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9856_cancers-12-03457-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9856_cancers-12-03457-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9856_cancers-12-03457-g002.jpg?time\u003d20240507','sortOrder':'34','description':'Figure 2 Transcriptomic profiling of pre-metastatic lungs demonstrates an inflammatory response and myeloid cell infiltration. ( A ) Volcano plot of differentially expressed genes in pre-metastatic lungs compared to control lungs. Dots colored in red or blue represent upregulated and downregulated genes, respectively, meeting the thresholds of fold change \u003e1.5, p -value \u003c 0.01, and FDR \u003c0.2 respectively. ( B ) Heatmap representation of upregulated genes under the GO term \'\'inflammatory response\'\' (GO:0006954) in tumor-bearing mice vs. control mice. Each column represents one biological replicate ( n \u003d 6 in the tumor group (T1-T6), n \u003d 4 in the control group (PBS1-PBS4)). Cut-off values of fold change are \u003e1.5 and FDR \u003c0.2. The clustering separates the genes by color with positive or negative row-scaled Z-scores represented in red and blue, respectively. ( C ) Representative immunofluorescence images (left panel) and quantification (right panel) of CD11b + cells (red) and nuclei (blue) in lungs of control mice (day 12 and day 28) and tumor-bearing mice (day 12 and day 28), respectively. Scale bar, 500 um. (n.s., non-significant, *, p \u003c 0.05, ***, p \u003d 0.001; Bonferroni\u0027\u0027s one-way ANOVA test).','shortDescription':'Figure 2 Transcriptomic profiling of pre-metastatic lungs demonstrates an inflammatory response and myeloid cell infiltration. ( A ) Volcano plot of differentially expressed genes in pre-metastatic lungs compared to control lungs. Dots colored in red or blue represent upregulated and downregulated genes, respectively, meeting the thresholds of fold change \u003e1.5, p -value \u003c 0.01, and FDR \u003c0.2 respectively. ( ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Nov 20, 2020, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Cancers','journalText':'Cancers 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9856_cancers-12-03457-g002.jpg','benchSciPubmedId':'33233625'},{'sku':'63-0112-82','imageId':'853341','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9856_cancers-12-03457-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9856_cancers-12-03457-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9856_cancers-12-03457-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9856_cancers-12-03457-g003.jpg?time\u003d20240507','sortOrder':'35','description':'Figure 3 Osteosarcoma cell-derived extracellular-vesicles (EVs) promote accumulation of bone marrow-derived cells in the lungs. ( A ) Schematic illustration of the experimental setup. Mice were treated with either PBS, 143-B EVs or SAOS-2 EVs (10 ug) 2 times a week for 3 weeks after which mice were euthanized. ( B ) Relative mRNA expression levels of integrin alpha M gene encoding CD11b ( Itgam ) in bulk lung RNA in mice of indicated treatment groups. Transcript levels are normalized to Gapdh and expressed as fold change relative to control mice. n \u003d 6, mice were pooled from two independent experiments indicated in red and black ( C ) Representative flow cytometric profile (left panel) and quantification (right panel) of CD11b + cells isolated from lungs of mice from indicated treatment groups; n \u003d 3 mice per group. ( D ) Representative immunofluorescence images (left panel) and quantification (right panel) of CD11b + cells (red) and nuclei (blue) in lungs of mice from indicated treatment groups. Scale bar, 500 um. (*, p \u003c 0.05, **, p \u003c 0.01, ***, p \u003d 0.0001, ****, p \u003c 0.0001, Bonferroni\u0027\u0027s one-way ANOVA test).','shortDescription':'Figure 3 Osteosarcoma cell-derived extracellular-vesicles (EVs) promote accumulation of bone marrow-derived cells in the lungs. ( A ) Schematic illustration of the experimental setup. Mice were treated with either PBS, 143-B EVs or SAOS-2 EVs (10 ug) 2 times a week for 3 weeks after which mice were euthanized. ( B ) Relative mRNA expression levels of integrin alpha M gene encoding CD11b ( Itgam ) in bulk lu... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)','title':'CD11b Antibody (63-0112-82) in IHC, Flow','appAbv':'Flow','dateTime':'Nov 20, 2020, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Cancers','journalText':'Cancers 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9856_cancers-12-03457-g003.jpg','benchSciPubmedId':'33233625'},{'sku':'63-0112-82','imageId':'853307','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9855_cells-09-02344-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9855_cells-09-02344-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9855_cells-09-02344-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9855_cells-09-02344-g005.jpg?time\u003d20240507','sortOrder':'36','description':'Figure 5 Immunofluorescent analysis of Lrrk2 distribution and subcellular localization in mBAC-Lrrk2-G2019S brain. Representative co-immunofluorescent staining of brain slices from hemizygous transgenic mice overexpressing murine BAC-Lrrk2-G2019S. In detail: ( a , b ) tot-Lrrk2 (green) distribution in cortex and striatum and subcellular localization in beta-III-tubulin positive neurons (red) of 1-month-old and 12-months-old mice; ( c , d ) tot-Lrrk2 (red) distribution in cortex and striatum and subcellular localization in GLT-1 positive astrocytes (green) of 1-month-old and 12-months-old mice; ( e , f ) tot-Lrrk2 (red) distribution in cortex and striatum and subcellular localization in CD11b positive microglia (green) of 1-month-old and 12-months-old mice. Representative immunofluorescent staining of ( g , h ) GFAP and ( i , j ) CD11b-positive cells highlighting the different shape and activation level of striatal astrocytes and microglia respectively, at 1 and 12 months of age. n \u003d 3 animals each staining. Scale bars 200 mum, and 50 mum.','shortDescription':'Figure 5 Immunofluorescent analysis of Lrrk2 distribution and subcellular localization in mBAC-Lrrk2-G2019S brain. Representative co-immunofluorescent staining of brain slices from hemizygous transgenic mice overexpressing murine BAC-Lrrk2-G2019S. In detail: ( a , b ) tot-Lrrk2 (green) distribution in cortex and striatum and subcellular localization in beta-III-tubulin positive neurons (red) of 1-month-old ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))','title':'CD11b Antibody (63-0112-82) in IHC, IHC (PFA)','appAbv':'IHC','dateTime':'Oct 22, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Cells','journalText':'Cells 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9855_cells-09-02344-g005.jpg','benchSciPubmedId':'33105882'},{'sku':'63-0112-82','imageId':'879533','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14972_cancers-12-03072-g006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14972_cancers-12-03072-g006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14972_cancers-12-03072-g006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14972_cancers-12-03072-g006.jpg?time\u003d20240507','sortOrder':'37','description':'Figure 6 IL-15 GET induces immune cell infiltrate into the tumor microenvironment. Haematoxylin and eosin stain. Anti-CD3e (green-T cells), anti-CD11b (green-APCs), and anti-CD335 (green--natural killer cells) staining (green) reveal the presence of immune cells within the tumors of mice treated using EP2 to deliver pAG115, p,G170, and pAG208 on days 0, 4, and 7. Tissue was collected on day 8 (24 h after the third treatment). Images are the best representative sample of n \u003d 2 for each group. Sections were counterstained with a nuclear dye 4\u0027,6-diamidino-2-phenylindole (DAPI) (Blue). Mag \u003d 20x. Scale bar \u003d 500 um.','shortDescription':'Figure 6 IL-15 GET induces immune cell infiltrate into the tumor microenvironment. Haematoxylin and eosin stain. Anti-CD3e (green-T cells), anti-CD11b (green-APCs), and anti-CD335 (green--natural killer cells) staining (green) reveal the presence of immune cells within the tumors of mice treated using EP2 to deliver pAG115, p,G170, and pAG208 on days 0, 4, and 7. Tissue was collected on day 8 (24 h after th... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 21, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Cancers','journalText':'Cancers 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14972_cancers-12-03072-g006.jpg','benchSciPubmedId':'33096755'},{'sku':'63-0112-82','imageId':'925942','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19066_db191239f2.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19066_db191239f2.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19066_db191239f2.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19066_db191239f2.jpg?time\u003d20240507','sortOrder':'38','description':'Figure 2 Adipocyte Becn1 deficiency promotes adipose tissue inflammation. A : Histological analysis of eWAT from 10-week-old WT and AKO mice. WAT sections were examined by H\u0026E staining. Black arrows indicate CLSs. Scale bars, 50 um. B : Quantification of CLSs in WT and AKO mice ( n \u003d 8). C : The mRNA expression levels of F4/80 , Cd11b , and Cd11c in eWAT from 10-week-old WT and AKO mice ( n \u003d 7). D : Representative immunofluorescence images of eWAT from 10-week-old WT and AKO mice. The eWAT sections were stained with CD11b (cyan) and CD11c (red) antibodies. Nucleus was stained with DAPI (blue). Scale bars, 75 um. E : The mRNA expression of M1 and M2 macrophage-specific markers in eWAT from 10-week-old WT and AKO mice ( n \u003d 7). F : Total number of F4/80 + CD11b + SVCs per gram of eWAT was determined by flow cytometry in WT and AKO mice ( n \u003d 3). G : The percentages of CD11c + and CD11c - CD206 + in F4/80 + CD11b + cells were measured by flow cytometry in the SVCs of WT and AKO mice ( n \u003d 3). The qRT-PCR results were normalized to Ppia . Data are presented as mean +- SEM. * P \u003c 0.05; ** P \u003c 0.01; *** P \u003c 0.001; # P \u003d 0.12. exp., expression; FM, fat mass.','shortDescription':'Figure 2 Adipocyte Becn1 deficiency promotes adipose tissue inflammation. A : Histological analysis of eWAT from 10-week-old WT and AKO mice. WAT sections were examined by H\u0026E staining. Black arrows indicate CLSs. Scale bars, 50 um. B : Quantification of CLSs in WT and AKO mice ( n \u003d 8). C : The mRNA expression levels of F4/80 , Cd11b , and Cd11c in eWAT from 10-week-old WT and AKO mice ( n \u003d 7). D : Repres... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jan 1, 2021, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Diabetes','journalText':'Diabetes 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19066_db191239f2.jpg','benchSciPubmedId':'33046512'},{'sku':'63-0112-82','imageId':'988223','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24324_12FF3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24324_12FF3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24324_12FF3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24324_12FF3.jpg?time\u003d20240507','sortOrder':'39','description':'FIGURE 3. Navitoclax alleviates the inflammatory reaction induced by Ang II. A, Representative images of immunofluorescent staining of CD11B (red) and DAPI (blue), which show inflammatory cells of heart sections and quantification of the CD11B fluorescent area among the different groups. B, Representative western blotting bands of adhesion molecules and cytokine levels in the heart tissue of the mouse model and quantitative analysis of relative densities compared with GAPDH (fold). C, Protein expression of ICAM1, VCAM1, IL-6, and TNF-\u0026agr in vitro and quantitative analysis of relative densities compared with \u0026bgr-actin (fold). Scale bars \u003d 50 \u0026mgrm. \u0026ast P \u003c 0.05, \u0026ast\u0026ast P \u003c 0.01. All data in the bar graphs are shown as mean +- SEM with 4 mice per group. Navi, navitoclax.','shortDescription':'FIGURE 3. Navitoclax alleviates the inflammatory reaction induced by Ang II. A, Representative images of immunofluorescent staining of CD11B (red) and DAPI (blue), which show inflammatory cells of heart sections and quantification of the CD11B fluorescent area among the different groups. B, Representative western blotting bands of adhesion molecules and cytokine levels in the heart tissue of the mouse model... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 1, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of cardiovascular pharmacology','journalText':'Journal of cardiovascular pharmacology 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24324_12FF3.jpg','benchSciPubmedId':'32675749'},{'sku':'63-0112-82','imageId':'853153','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9850_pbio.3000734.s009.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9850_pbio.3000734.s009.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9850_pbio.3000734.s009.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9850_pbio.3000734.s009.jpg?time\u003d20240507','sortOrder':'40','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry and Immunohistochemistry (PFA fixed)','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry and Immunohistochemistry (PFA fixed)','altTag':'CD11b Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))','title':'CD11b Antibody (63-0112-82) in IHC, IHC (PFA)','appAbv':'IHC','dateTime':'Jun 1, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'PLoS biology','journalText':'PLoS biology 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9850_pbio.3000734.s009.jpg','benchSciPubmedId':'32502201'},{'sku':'63-0112-82','imageId':'925686','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19065_nihms-1546634-f0008.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19065_nihms-1546634-f0008.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19065_nihms-1546634-f0008.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19065_nihms-1546634-f0008.jpg?time\u003d20240507','sortOrder':'41','description':'Extended Data Fig. 3 | Stress-induced hair greying is not mediated through corticosterone or immune attack. a, Left, white hair formation after RTX injection in Rag1 mutant mice devoid of T and B cells (Rag1 KO, n \u003d 6 for each condition, two-tailed unpaired t -test). Right, immunofluorescent staining for T cell marker CD3 (green) in control and Rag1 KO skin (n \u003d 6 mice for each condition, two-tailed unpaired t -test). b, Left, hair greying occurs when RTX is injected into CD11b-DTR mice treated with diphtheria toxin (DT) to deplete myeloid cells (n \u003d 6 mice for each condition). Right, immunofluorescent staining for CD11b (green) in DT treated control and CD11b-DTR skin (n \u003d 6 mice for each condition). c, Expression of adrenergic receptors and glucocorticoid receptor (GR) in MeSCs (n \u003d 2 biologically independent samples). d, White hair formation following RTX injection into Tyr-CreER; GR fl/fl mice (MeSC-GR cKO; n \u003d 6 mice for each condition, two-tailed unpaired t -test). e, Left, enzyme-linked immunosorbent assay (ELISA) measurement of corticosterone level in the blood 3 days after supplying corticosterone in drinking water (n \u003d 4 mice for each condition). Middle, immunofluorescent staining of hair follicles for TRP2 (red) from mice 5 days after corticosterone treatment (n \u003d 30 HFs from 3 mice for each condition, two-tailed unpaired t -test). Right, hair coat colour after HFs in corticosterone-treated mice enter another round of anagen to regenerate new hairs. CORT: corticost','shortDescription':'Extended Data Fig. 3 | Stress-induced hair greying is not mediated through corticosterone or immune attack. a, Left, white hair formation after RTX injection in Rag1 mutant mice devoid of T and B cells (Rag1 KO, n \u003d 6 for each condition, two-tailed unpaired t -test). Right, immunofluorescent staining for T cell marker CD3 (green) in control and Rag1 KO skin (n \u003d 6 mice for each condition, two-tailed unpaire... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jan 1, 2020, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Nature','journalText':'Nature 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19065_nihms-1546634-f0008.jpg','benchSciPubmedId':'31969699'},{'sku':'63-0112-82','imageId':'523933','imageUrl':'https://www.thermofisher.com/antibody/images/650/enu0051827770006-29-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/enu0051827770006-29-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/enu0051827770006-29-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/enu0051827770006-29-20190221105219.jpg?time\u003d20240507','sortOrder':'42','description':'Figure 6. Microglia expressing pro-/anti-inflammatory markers. Representative confocal immunofluorescent microscopy of CD11b (red) and pro-/anti-inflammatory markers (green) within the lumbar spinal cord are shown (images are from a WT B6 mouse on day 1 post-L5Tx surgery). Merged panels show colocalization of CD11b with the corresponding inflammatory marker. Scale bar: 40 mum.','shortDescription':'Figure 6. Microglia expressing pro-/anti-inflammatory markers. Representative confocal immunofluorescent microscopy of CD11b (red) and pro-/anti-inflammatory markers (green) within the lumbar spinal cord are shown (images are from a WT B6 mouse on day 1 post-L5Tx surgery). Merged panels show colocalization of CD11b with the corresponding inflammatory marker. Scale bar: 40 mum.','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Apr 19, 2019, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'eNeuro','journalText':'eNeuro 2019 - ','sourceType':'3','benchsci':true,'imageName':'enu0051827770006-29-20190221105219.jpg','benchSciPubmedId':'30417077'},{'sku':'63-0112-82','imageId':'722126','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_5940_jciinsight-3-99722-g084.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_5940_jciinsight-3-99722-g084.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_5940_jciinsight-3-99722-g084.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_5940_jciinsight-3-99722-g084.jpg?time\u003d20240507','sortOrder':'43','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 18, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'JCI insight','journalText':'JCI insight 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_5940_jciinsight-3-99722-g084.jpg','benchSciPubmedId':'30333307'},{'sku':'63-0112-82','imageId':'523930','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g4.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g4.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_4791_ad-9-5-785-g4.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_4791_ad-9-5-785-g4.jpg?time\u003d20240507','sortOrder':'44','description':'Figure 4. Nrf2/HO-1/Gp91phox expression and Nrf2/HO-1/Gp91phox microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. Nrf2/HO-1/Gp91phox expression in brain was assayed by Western blot. A) Representative bands of Nrf2 immunostaining. B) Quantitative data of Nrf2 immunostaining. Nrf2/HO-1/Gp91phox positive microglia in brain was assayed by double immunohistochemistry of Nrf2/HO-1/Gp91phox and CD11b. C) Representative images of Nrf2 expression on CD11b + microglia. D) Quantitative data of double positive cells. E) Representative bands of HO-1 immunostaining. F) quantitative data of HO-1 immunostaining; G) Representative images of HO-1 expression on CD11b + microglia. H) Quantitative data of double positive cells; I) Representative bands of gp91phox immunostaining. J) Quantitative data of gp91phox immunostaining. K) Representative images of gp91phox expression on CD11b + microglia. L) Quantitative data of double positive cells. Quantitative results are mean +- SEM of 4 mice in each group. * p \u003c0.05, ** p \u003c0.01, ***p\u003c0.001.','shortDescription':'Figure 4. Nrf2/HO-1/Gp91phox expression and Nrf2/HO-1/Gp91phox microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. Nrf2/HO-1/Gp91phox expression in brain was assayed by Western blot. A) Representative bands of Nrf2 immunostaining. B) Quantitative data of Nrf2 immunostaining. Nrf2... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 1, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Aging and disease','journalText':'Aging and disease 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_4791_ad-9-5-785-g4.jpg','benchSciPubmedId':'30271656'},{'sku':'63-0112-82','imageId':'523929','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_4791_ad-9-5-785-g3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_4791_ad-9-5-785-g3.jpg?time\u003d20240507','sortOrder':'45','description':'Figure 3. The levels of TNF-alpha, IL-1beta, iNOS/Arg-1 expression, and iNOS/Arg-1 microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. The levels of TNF-alpha and IL-1beta in brain were assayed by ELISA. A) TNF-alpha; and B) IL-1beta expression in brain was assayed by Western blot. C) Representative bands of iNOS immunostaining. D) Quantitative data of iNOS immunostaining. iNOS/Arg-1 positive microglia in brain was assayed by double immunohistochemistry of iNOS/Arg-1 and CD11b. E) Representative images of iNOS expression on CD11b + microglia. F) Quantitative data of double positive cells. G) Representative bands of Arg-1 immunostaining. H) Quantitative data of Arg-1 immunostaining. I) Representative images of Arg-1 expression on CD11b + microglia. J) Quantitative data of double positive cells. Quantitative results are mean +- SEM of 4 mice in each group. * p \u003c0.05, ** p \u003c0.01.','shortDescription':'Figure 3. The levels of TNF-alpha, IL-1beta, iNOS/Arg-1 expression, and iNOS/Arg-1 microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. The levels of TNF-alpha and IL-1beta in brain were assayed by ELISA. A) TNF-alpha; and B) IL-1beta expression in brain was assayed by Western blo... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 1, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Aging and disease','journalText':'Aging and disease 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_4791_ad-9-5-785-g3.jpg','benchSciPubmedId':'30271656'},{'sku':'63-0112-82','imageId':'523928','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g2.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g2.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_4791_ad-9-5-785-g2.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_4791_ad-9-5-785-g2.jpg?time\u003d20240507','sortOrder':'46','description':'Figure 2. TLR2/p-NF-kappaB/p65 expression and TLR2/p-NF-kappaB/p65 microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. TLR2/p-NF-kappaB/p65 expression in brain was assayed by Western blot. A) Representative bands of TLR2 immunostaining. B) Quantitative data of TLR2 immunostaining. TLR2/p-NF-kappaB/p65 positive microglia in brain was assayed by double immunohistochemistry of TLR2/p-NF-kappaB/p65 and CD11b. C) Representative images of TLR2 expression on CD11b + microglia. D) Quantitative data of double positive cells. E) Representative bands of p-NF-kappaB/p65 immunostaining. F) Quantitative data of p-NF-kappaB/p65 immunostaining. G) Representative images of p-NF-kappaB/p65 expression on CD11b + microglia. H) Quantitative data of double positive cells. Quantitative results are mean +- SEM of 4 mice in each group. * p \u003c0.05, ** p \u003c0.01.','shortDescription':'Figure 2. TLR2/p-NF-kappaB/p65 expression and TLR2/p-NF-kappaB/p65 microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. TLR2/p-NF-kappaB/p65 expression in brain was assayed by Western blot. A) Representative bands of TLR2 immunostaining. B) Quantitative data of TLR2 immunostaining... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 1, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Aging and disease','journalText':'Aging and disease 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_4791_ad-9-5-785-g2.jpg','benchSciPubmedId':'30271656'},{'sku':'63-0112-82','imageId':'523926','imageUrl':'https://www.thermofisher.com/antibody/images/650/12974_2018_1207_Fig2_HTML-44-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/12974_2018_1207_Fig2_HTML-44-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/12974_2018_1207_Fig2_HTML-44-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/12974_2018_1207_Fig2_HTML-44-20190221105219.jpg?time\u003d20240507','sortOrder':'47','description':'Fig. 2 Vascular congestion in IL-10 KO mice with malaria includes thrombi-containing monocytes and T cells. Immunofluorescent staining of fixed, frozen brain sections (30 mum) from P. chabaudi -infected IL-10 KO mice (day 8 p.i., n \u003d 4 mice). a Confocal images (x 40) of IL-10 KO brain stained for fibrin (red). b Successive single-plane confocal images (x 40) of a 30-mum z-stack showing complete occlusion of a large vessel with residual fibrinogen (red). c Immunofluorescence staining of IL-10 KO brains showing fibrin staining of blood vessels (red) and leukocytes expressing CD45 (green, x 60) and d CD11b (green, x 40). e CTV + CD4 T cells (blue) from infected IL-10 KO mice were adoptively transferred into infection-matched IL-10 KO (day 7 p.i.) recipients 3.5 h before sacrifice. Frozen brain sections (day 7 p.i.) were stained for fibrin (red). Max intensity projection of a 30-mum z-stack (x 240) displayed from brain tissue of IL-10 KO mice co-stained with WT control samples ( n \u003d 3-4 mice per group). Scale bars represent 30 mum ( a ), 50 mum ( b - d ), or 10 mum ( d )','shortDescription':'Fig. 2 Vascular congestion in IL-10 KO mice with malaria includes thrombi-containing monocytes and T cells. Immunofluorescent staining of fixed, frozen brain sections (30 mum) from P. chabaudi -infected IL-10 KO mice (day 8 p.i., n \u003d 4 mice). a Confocal images (x 40) of IL-10 KO brain stained for fibrin (red). b Successive single-plane confocal images (x 40) of a 30-mum z-stack showing complete occlu... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jun 4, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of neuroinflammation','journalText':'Journal of neuroinflammation 2018 - ','sourceType':'3','benchsci':true,'imageName':'12974_2018_1207_Fig2_HTML-44-20190221105219.jpg','benchSciPubmedId':'29866139'},{'sku':'63-0112-82','imageId':'722096','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_5918_pnas.1722020115fig03.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_5918_pnas.1722020115fig03.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_5918_pnas.1722020115fig03.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_5918_pnas.1722020115fig03.jpg?time\u003d20240507','sortOrder':'48','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry and Flow Cytometry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry and Flow Cytometry','altTag':'CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)','title':'CD11b Antibody (63-0112-82) in IHC, Flow','appAbv':'Flow','dateTime':'May 1, 2018, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Proceedings of the National Academy of Sciences of the United States of America','journalText':'Proceedings of the National Academy of Sciences of the United States of America 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_5918_pnas.1722020115fig03.jpg','benchSciPubmedId':'29666270'},{'sku':'63-0112-82','imageId':'840874','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12614_nihms950531f3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12614_nihms950531f3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12614_nihms950531f3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12614_nihms950531f3.jpg?time\u003d20240507','sortOrder':'49','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jun 15, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of neuroimmunology','journalText':'Journal of neuroimmunology 2018 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12614_nihms950531f3.jpg','benchSciPubmedId':'29526406'},{'sku':'63-0112-82','imageId':'341964','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_4189_fphys-09-00047-g0013.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_4189_fphys-09-00047-g0013.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_4189_fphys-09-00047-g0013.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_4189_fphys-09-00047-g0013.jpg?time\u003d20240507','sortOrder':'50','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Sep 30, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Frontiers in physiology','journalText':'Frontiers in physiology 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_4189_fphys-09-00047-g0013.jpg','benchSciPubmedId':'29483875'},{'sku':'63-0112-82','imageId':'722085','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6068_12974_2017_990_Fig2_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6068_12974_2017_990_Fig2_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6068_12974_2017_990_Fig2_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6068_12974_2017_990_Fig2_HTML.jpg?time\u003d20240507','sortOrder':'51','description':'Fig. 2 Intracerebroventricular (ICV) administration of IL-1beta induces NF-kappaB nuclear localization in a time- and region-dependent manner. The effect of ICV IL-1beta is both rapid and transient as demonstrated by NF-kappaB immunoreactivity (IR) in representative confocal images of the paraventricular nucleus (PVN). IR in vehicle (aCSF, n \u003d 6)-treated animals showed cytoplasmic labeling of blood vessels ( a ). IL-1beta treatment caused NF-kappaB nuclear localization that peaked by 30 min ( b , n \u003d 8), persisted for at least 2 h ( c , n \u003d 4) and returned to near baseline, with cytoplasmic vascular patterns reappearing, by 4 h after treatment ( d , n \u003d 4). This effect was most prominent in and around the PVN, organum vasculosum lamina terminalis (OVLT; e , f ), subfornical organ (SFO; g , h ), arcuate nucleus/median eminence (ARC/ME; i , j ), and choroid plexus (ChP; k , l ). High-magnification confocal images of co-labeling with cell-specific markers shows that vascular endothelial cells ( m ), microglia ( n ), astrocytes ( o ), and tanycytes ( p ) directly respond to IL-1beta. Scale bars: a - l \u003d 50 mum, m - p \u003d 10 mum','shortDescription':'Fig. 2 Intracerebroventricular (ICV) administration of IL-1beta induces NF-kappaB nuclear localization in a time- and region-dependent manner. The effect of ICV IL-1beta is both rapid and transient as demonstrated by NF-kappaB immunoreactivity (IR) in representative confocal images of the paraventricular nucleus (PVN). IR in vehicle (aCSF, n \u003d 6)-treated animals showed cytoplasmic labeling of blood vessels ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Nov 9, 2017, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of neuroinflammation','journalText':'Journal of neuroinflammation 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6068_12974_2017_990_Fig2_HTML.jpg','benchSciPubmedId':'29121947'},{'sku':'63-0112-82','imageId':'880609','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14956_kaup-13-12-1380757-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14956_kaup-13-12-1380757-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14956_kaup-13-12-1380757-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14956_kaup-13-12-1380757-g003.jpg?time\u003d20240507','sortOrder':'52','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jul 5, 2019, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Autophagy','journalText':'Autophagy 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14956_kaup-13-12-1380757-g003.jpg','benchSciPubmedId':'28933598'},{'sku':'63-0112-82','imageId':'329966','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12605_41598_2017_8535_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12605_41598_2017_8535_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12605_41598_2017_8535_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12605_41598_2017_8535_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'53','description':'Figure 1 Upregulation of HDAC3 in microglia/macrophages after SCI. ( a ) Representative sagittal immunohistochemistry images and quantification demonstrate upregulation of HDAC3 (green) at lesion center 7 days post injury (7 dpi) by T8 dorsal column transection (SCI-T). DAPI nuclear counterstaining highlights the increased cellularity at lesion core. Enlarged images of boxed areas are shown on the right. Quantification of the number of HDAC3+ cells at the injury site is shown on the right: *p \u003d 0.015, parametric slit-plot ANOVA. 3 pairs of mice were examined, with 3 representative images quantified for each animal. ( b ) Representative immunohistochemistry images of HDAC3 at different time points after SCI-T as compared to sham. ( c ) Representative immunofluorescent images of co-immunolabeling show a large overlap of HDAC3+ cells (green) and CD11b+ or Iba1+ immune cells (red) at the injury site at 7 days after SCI-T. Enlarged images of boxed areas are shown on the right. ( d ) Immunohistochemistry images for CD11b (red) and different class I HDACs (green) at 7 days after SCI-T. No significant overlaps were detected between these HDACs and CD11b. Orientation: R: Rostral, C: Caudal, D: Dorsal, V: Ventral. Scale bars: 100 um ( a , b , c ), 25 um ( d , enlarged images in a , c ).','shortDescription':'Figure 1 Upregulation of HDAC3 in microglia/macrophages after SCI. ( a ) Representative sagittal immunohistochemistry images and quantification demonstrate upregulation of HDAC3 (green) at lesion center 7 days post injury (7 dpi) by T8 dorsal column transection (SCI-T). DAPI nuclear counterstaining highlights the increased cellularity at lesion core. Enlarged images of boxed areas are shown on the right. Qu... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Aug 17, 2017, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12605_41598_2017_8535_Fig1_HTML.jpg','benchSciPubmedId':'28819194'},{'sku':'63-0112-82','imageId':'523924','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_3470_oncotarget-08-95-g008.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_3470_oncotarget-08-95-g008.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_3470_oncotarget-08-95-g008.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_3470_oncotarget-08-95-g008.jpg?time\u003d20240507','sortOrder':'54','description':'Figure 8 Berberine increased neutrophil recruitment in the peritoneal cavity and the colon C57BL/6 mice were administered (i.g.) with berberine (100 mg/kg body weight) or vehicle (2% Tween-80 in PBS) once a day for 3 consecutive days prior to injection (i.p.) with viable E. coli (2x10 9 CFU/mouse). After indicated time periods, cells in the peritoneal cavity were harvested by 1.5 ml PBS and double stained with FITC-CD11b and PerCP-Gr-1 antibodies for flow cytometric analysis, and the colon was collected and fixed in 4% neutral formaldehyde for histological analysis. A. Representative flow cytometric dot-plots of three independent experiments. B. Quantification of CD11b + Gr-1 + cells (neutrophils) within the gate in A. Data are expressed as mean +- SD ( n \u003d 3). * P \u003c 0.05; *** P \u003c 0.001; ns, not significant. C. Hematoxylin and eosin (H\u0026E) staining of the colon sections 8 h after infection ( n \u003d 3). Representative images from each mouse are shown. Arrows indicate infiltrated inflammatory cells in the colon tissues. Scale bar, 100 mum; BBR, berberine.','shortDescription':'Figure 8 Berberine increased neutrophil recruitment in the peritoneal cavity and the colon C57BL/6 mice were administered (i.g.) with berberine (100 mg/kg body weight) or vehicle (2% Tween-80 in PBS) once a day for 3 consecutive days prior to injection (i.p.) with viable E. coli (2x10 9 CFU/mouse). After indicated time periods, cells in the peritoneal cavity were harvested by 1.5 ml PBS and double stained w... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jan 3, 2017, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Oncotarget','journalText':'Oncotarget 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_3470_oncotarget-08-95-g008.jpg','benchSciPubmedId':'27980220'},{'sku':'63-0112-82','imageId':'312456','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_27840922_IF_13_MMR-14-06-4947-g04_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_27840922_IF_13_MMR-14-06-4947-g04_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_27840922_IF_13_MMR-14-06-4947-g04_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_27840922_IF_13_MMR-14-06-4947-g04_20180524.jpg?time\u003d20240507','sortOrder':'55','description':'Figure 5. RI inhibited microglia activation in mice with MPTP-induced parkinsonism. (A) ROCKII-positive and CD11b-positive staining is increased in the SN in mice with MPTP-induced PD compared with the control mice (magnification x20). The majority of ROCKII-positive cells were CD11b-positive. (B) CD11b-positive staining declined markedly in the RI mice as ROCKII-positive staining declined (magnification, x20). ROCKII, Rho/Rho-associated, coiled-coil-containing protein kinase II; RI, ROCKII interference; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; PD, Parkinson\u0026apos;s disease; CD11b, cluster of differentiation 11b; SN, substantia nigra.','shortDescription':'Figure 5. RI inhibited microglia activation in mice with MPTP-induced parkinsonism. (A) ROCKII-positive and CD11b-positive staining is increased in the SN in mice with MPTP-induced PD compared with the control mice (magnification x20). The majority of ROCKII-positive cells were CD11b-positive. (B) CD11b-positive staining declined markedly in the RI mice as ROCKII-positive staining declined (magnification, x... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Dec 1, 2016, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Molecular medicine reports','journalText':'Molecular medicine reports 2016 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_27840922_IF_13_MMR-14-06-4947-g04_20180524.jpg','benchSciPubmedId':'27840922'},{'sku':'63-0112-82','imageId':'353862','imageUrl':'https://www.thermofisher.com/antibody/images/650/11011282-27065324-CD11b-nihms852440f3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/11011282-27065324-CD11b-nihms852440f3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/11011282-27065324-CD11b-nihms852440f3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/11011282-27065324-CD11b-nihms852440f3.jpg?time\u003d20240507','sortOrder':'57','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Oct 27, 2016, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Oncogene','journalText':'Oncogene 2016 - ','sourceType':'3','benchsci':true,'imageName':'11011282-27065324-CD11b-nihms852440f3.jpg','benchSciPubmedId':'27065324'},{'sku':'63-0112-82','imageId':'333864','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_2970_dmm-9-022475-g1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_2970_dmm-9-022475-g1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_2970_dmm-9-022475-g1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_2970_dmm-9-022475-g1.jpg?time\u003d20240507','sortOrder':'58','description':'Fig. 1. Flow cytometry can quantify the leukocyte infiltrate during EIU and be used as a scoring system for identifying therapeutic effects. In the absence of disease, (A) the anterior chamber is unremarkable and (B) negligible numbers of CD45+CD11b+Ly6G+ cells are detected by flow cytometry of whole ocular tissues. Numbers within the graphs in B indicate the percentage of each parent gate. At 18 h following intravitreal delivery of LPS, (C) marked ocular infiltration occurs throughout the eye, with leukocyte sediment (hypopyon) seen in the anterior chamber (indicated by the red arrow). (D) Representative example of the gating strategy to identify CD45+CD11b+Ly6G+ and Ly6C+ cells. (E) Immunohistochemistry targeting the same epitopes as the flow cytometry antibodies confirms the presence of leukocytes in the anterior chamber (AC) and vitreous, staining for (F) CD45 and (G) CD11b. CE, ciliary epithelium. (H) Ly6G+ cells (magenta) are polymorphonuclear, consistent with neutrophils, whereas Ly6C+ cells (white) appear mononuclear, consistent with monocytes. Scale bars: 20 mum. (I) Compared with untreated eyes, a statistically significant elevation in the number of neutrophils (CD45+CD11b+Ly6G+) can be seen during EIU and is proposed as a single measure score. Mann-Whitney U -test, n \u003d20. (J) Using multicolour flow cytometry, the average cellular composition of the infiltrate in EIU was determined. Mean values from eight eyes undergoing standard processing. See Fig. S2 for the gati','shortDescription':'Fig. 1. Flow cytometry can quantify the leukocyte infiltrate during EIU and be used as a scoring system for identifying therapeutic effects. In the absence of disease, (A) the anterior chamber is unremarkable and (B) negligible numbers of CD45+CD11b+Ly6G+ cells are detected by flow cytometry of whole ocular tissues. Numbers within the graphs in B indicate the percentage of each parent gate. At 18 h followin... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)','title':'CD11b Antibody (63-0112-82) in IHC, Flow','appAbv':'Flow','dateTime':'Apr 1, 2016, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Disease models \u0026 mechanisms','journalText':'Disease models \u0026 mechanisms 2016 - ','sourceType':'3','benchsci':true,'imageName':'tfs_2970_dmm-9-022475-g1.jpg','benchSciPubmedId':'26794131'},{'sku':'63-0112-82','imageId':'357122','imageUrl':'https://www.thermofisher.com/antibody/images/650/13011282-26658452-CD11b-NIHMS727001-supplement-2.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/13011282-26658452-CD11b-NIHMS727001-supplement-2.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/13011282-26658452-CD11b-NIHMS727001-supplement-2.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/13011282-26658452-CD11b-NIHMS727001-supplement-2.jpg?time\u003d20240507','sortOrder':'59','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Mar 1, 2016, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Laboratory investigation; a journal of technical methods and pathology','journalText':'Laboratory investigation; a journal of technical methods and pathology 2016 - ','sourceType':'3','benchsci':true,'imageName':'13011282-26658452-CD11b-NIHMS727001-supplement-2.jpg','benchSciPubmedId':'26658452'},{'sku':'63-0112-82','imageId':'318355','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12590_13058_2015_558_Fig6_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12590_13058_2015_558_Fig6_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12590_13058_2015_558_Fig6_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12590_13058_2015_558_Fig6_HTML.jpg?time\u003d20240507','sortOrder':'60','description':'Figure 6 Ly-6G + cell depletion decreases the establishment and growth of liver metastases. (A) Schematic depicting the experimental protocol for depletion of Ly-6G + cells. (B) Paraffin-embedded liver sections were obtained following completion of the experimental metastasis assays and subjected to immunohistochemical staining with anti-Ly-6G antibodies. Statistically significant decreases in Ly-6G + cells that infiltrate the liver were observed when the neutrophil-depleted cohort was compared with the isotype control cohort ( P \u003d 0.045). Representative images of Ly-6G-stained liver sections from each cohort. (C) Quantification of the tumor burden (tumor area/tissue area) within the cardiac liver lobe following splenic injection of 2776 liver-aggressive cells. Statistically significant decreases in liver-metastatic burden were observed when the isotype control cohort was compared with the neutrophil-depleted cohort ( P \u003d 0.031). Representative images of hematoxylin and eosin (H\u0026E)-stained liver sections exhibiting the liver-metastatic burden in each cohort. Dotted lines circumscribe breast cancer metastatic lesions within the liver. Scale bar represents 2 mm and applies to all panels. IP: intraperitoneal injection.','shortDescription':'Figure 6 Ly-6G + cell depletion decreases the establishment and growth of liver metastases. (A) Schematic depicting the experimental protocol for depletion of Ly-6G + cells. (B) Paraffin-embedded liver sections were obtained following completion of the experimental metastasis assays and subjected to immunohistochemical staining with anti-Ly-6G antibodies. Statistically significant decreases in Ly-6G + cells... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Mar 27, 2015, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Breast cancer research : BCR','journalText':'Breast cancer research : BCR 2015 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12590_13058_2015_558_Fig6_HTML.jpg','benchSciPubmedId':'25882816'},{'sku':'63-0112-82','imageId':'335802','imageUrl':'https://www.thermofisher.com/antibody/images/650/14011282-25849774-CD11b-nihms662893f8.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/14011282-25849774-CD11b-nihms662893f8.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/14011282-25849774-CD11b-nihms662893f8.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/14011282-25849774-CD11b-nihms662893f8.jpg?time\u003d20240507','sortOrder':'61','description':'Extended Data Fig. 4 Professional phagocytes are not present in regressing hair follicles a) Professional phagocytes do not enter regressing hair follicles. Single optical sections showing absence of myeloid populations inside the hair follicle 2.5 h after epithelial cell death (arrowhead), in LysM-Cre; tdTomato;K14-H2BGFP mice. b) Immunofluorescent staining of myeloid populations in skin during hair follicle regression. DAPI (blue), CD11b (red), and P-cadherin (green). Observations shown represent n \u003d 4 mice. Scale bar, 25 mum','shortDescription':'Extended Data Fig. 4 Professional phagocytes are not present in regressing hair follicles a) Professional phagocytes do not enter regressing hair follicles. Single optical sections showing absence of myeloid populations inside the hair follicle 2.5 h after epithelial cell death (arrowhead), in LysM-Cre; tdTomato;K14-H2BGFP mice. b) Immunofluorescent staining of myeloid populations in skin during hair follic... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jun 4, 2015, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Nature','journalText':'Nature 2015 - ','sourceType':'3','benchsci':true,'imageName':'14011282-25849774-CD11b-nihms662893f8.jpg','benchSciPubmedId':'25849774'},{'sku':'63-0112-82','imageId':'337552','imageUrl':'https://www.thermofisher.com/antibody/images/650/12031_2014_457_Fig4_HTML-20180918151446.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/12031_2014_457_Fig4_HTML-20180918151446.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/12031_2014_457_Fig4_HTML-20180918151446.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/12031_2014_457_Fig4_HTML-20180918151446.jpg?time\u003d20240507','sortOrder':'62','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Apr 1, 2015, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of molecular neuroscience : MN','journalText':'Journal of molecular neuroscience : MN 2015 - ','sourceType':'3','benchsci':true,'imageName':'12031_2014_457_Fig4_HTML-20180918151446.jpg','benchSciPubmedId':'25384918'},{'sku':'63-0112-82','imageId':'347820','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_2630_nihms-702371-f0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_2630_nihms-702371-f0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_2630_nihms-702371-f0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_2630_nihms-702371-f0003.jpg?time\u003d20240507','sortOrder':'63','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Aug 12, 2014, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Science signaling','journalText':'Science signaling 2014 - ','sourceType':'3','benchsci':true,'imageName':'tfs_2630_nihms-702371-f0003.jpg','benchSciPubmedId':'25118327'},{'sku':'63-0112-82','imageId':'339924','imageUrl':'https://www.thermofisher.com/antibody/images/650/auto-10-972-g4-20180918151446.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/auto-10-972-g4-20180918151446.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/auto-10-972-g4-20180918151446.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/auto-10-972-g4-20180918151446.jpg?time\u003d20240507','sortOrder':'64','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jun 1, 2014, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Autophagy','journalText':'Autophagy 2014 - ','sourceType':'3','benchsci':true,'imageName':'auto-10-972-g4-20180918151446.jpg','benchSciPubmedId':'24879148'},{'sku':'63-0112-82','imageId':'357656','imageUrl':'https://www.thermofisher.com/antibody/images/650/14011282-24849347-CD11b-zns9991456590001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/14011282-24849347-CD11b-zns9991456590001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/14011282-24849347-CD11b-zns9991456590001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/14011282-24849347-CD11b-zns9991456590001.jpg?time\u003d20240507','sortOrder':'65','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'May 21, 2014, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'The Journal of neuroscience : the official journal of the Society for Neuroscience','journalText':'The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 - ','sourceType':'3','benchsci':true,'imageName':'14011282-24849347-CD11b-zns9991456590001.jpg','benchSciPubmedId':'24849347'},{'sku':'63-0112-82','imageId':'664655','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_5243_nihms562523f7.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_5243_nihms562523f7.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_5243_nihms562523f7.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_5243_nihms562523f7.jpg?time\u003d20240507','sortOrder':'66','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Mar 4, 2014, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Cell metabolism','journalText':'Cell metabolism 2014 - ','sourceType':'3','benchsci':true,'imageName':'tfs_5243_nihms562523f7.jpg','benchSciPubmedId':'24606903'},{'sku':'63-0112-82','imageId':'357645','imageUrl':'https://www.thermofisher.com/antibody/images/650/14011282-24583879-CD11b-zh50091465210005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/14011282-24583879-CD11b-zh50091465210005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/14011282-24583879-CD11b-zh50091465210005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/14011282-24583879-CD11b-zh50091465210005.jpg?time\u003d20240507','sortOrder':'67','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Apr 15, 2014, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'American journal of physiology. Lung cellular and molecular physiology','journalText':'American journal of physiology. Lung cellular and molecular physiology 2014 - ','sourceType':'3','benchsci':true,'imageName':'14011282-24583879-CD11b-zh50091465210005.jpg','benchSciPubmedId':'24583879'},{'sku':'63-0112-82','imageId':'358596','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12584_3506fig1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12584_3506fig1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12584_3506fig1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12584_3506fig1.jpg?time\u003d20240507','sortOrder':'68','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Dec 1, 2013, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of lipid research','journalText':'Journal of lipid research 2013 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12584_3506fig1.jpg','benchSciPubmedId':'24133193'},{'sku':'63-0112-82','imageId':'362092','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12582_zbc0311354400010.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12582_zbc0311354400010.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12582_zbc0311354400010.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12582_zbc0311354400010.jpg?time\u003d20240507','sortOrder':'69','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jul 19, 2013, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2013 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12582_zbc0311354400010.jpg','benchSciPubmedId':'23720750'},{'sku':'63-0112-82','imageId':'357111','imageUrl':'https://www.thermofisher.com/antibody/images/650/13011282-22700709-CD11b-i1552-5783-53-8-4575-f05.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/13011282-22700709-CD11b-i1552-5783-53-8-4575-f05.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/13011282-22700709-CD11b-i1552-5783-53-8-4575-f05.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/13011282-22700709-CD11b-i1552-5783-53-8-4575-f05.jpg?time\u003d20240507','sortOrder':'70','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jul 9, 2012, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Investigative ophthalmology \u0026 visual science','journalText':'Investigative ophthalmology \u0026 visual science 2012 - ','sourceType':'3','benchsci':true,'imageName':'13011282-22700709-CD11b-i1552-5783-53-8-4575-f05.jpg','benchSciPubmedId':'22700709'},{'sku':'63-0112-82','imageId':'352003','imageUrl':'https://www.thermofisher.com/antibody/images/650/CD11b_22440612_IF_12_nihms361163f1_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/CD11b_22440612_IF_12_nihms361163f1_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/CD11b_22440612_IF_12_nihms361163f1_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/CD11b_22440612_IF_12_nihms361163f1_20180524.jpg?time\u003d20240507','sortOrder':'71','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Apr 4, 2012, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Cell metabolism','journalText':'Cell metabolism 2012 - ','sourceType':'3','benchsci':true,'imageName':'CD11b_22440612_IF_12_nihms361163f1_20180524.jpg','benchSciPubmedId':'22440612'},{'sku':'63-0112-82','imageId':'358584','imageUrl':'https://www.thermofisher.com/antibody/images/650/16011282-20488889-CD11b-awq107f2.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/16011282-20488889-CD11b-awq107f2.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/16011282-20488889-CD11b-awq107f2.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/16011282-20488889-CD11b-awq107f2.jpg?time\u003d20240507','sortOrder':'72','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Aug 1, 2010, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Brain : a journal of neurology','journalText':'Brain : a journal of neurology 2010 - ','sourceType':'3','benchsci':true,'imageName':'16011282-20488889-CD11b-awq107f2.jpg','benchSciPubmedId':'20488889'},{'sku':'63-0112-82','imageId':'487563','imageUrl':'https://www.thermofisher.com/antibody/images/650/nfig003_015-19-20190103084817.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/nfig003_015-19-20190103084817.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/nfig003_015-19-20190103084817.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/nfig003_015-19-20190103084817.jpg?time\u003d20240507','sortOrder':'73','description':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','shortDescription':'Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Sep 1, 2009, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Journal of cellular physiology','journalText':'Journal of cellular physiology 2009 - ','sourceType':'3','benchsci':true,'imageName':'nfig003_015-19-20190103084817.jpg','benchSciPubmedId':'19452445'},{'sku':'63-0112-82','imageId':'327107','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_1457_1472-6750-9-1-2.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_1457_1472-6750-9-1-2.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_1457_1472-6750-9-1-2.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_1457_1472-6750-9-1-2.jpg?time\u003d20240507','sortOrder':'74','description':'Figure 2 Survival of luciferase-expressing BMSC derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice . (A) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice. (B) Representative histological analysis of clonal BMSC-Luc grafts in the central nervous system of syngeneic immunocompetent mice. Week 1 post-implantation: Upper pictures, haematoxylin-eosin (HE) staining indicating localisation and general appearance of the implantation site. Lower left picture, Sca-1 staining indicating the BMSC origin of the observed cell graft. Lower right picture, CD11b staining indicating the presence of activated microglia surrounding the observed cell graft. Week 3 post-implantation: Upper pictures, HE staining indicating localisation and general appearance of the implantation site. Lower left picture, Sca-1 staining indicating the BMSC origin of the observed cell graft. Lower right picture, CD11b staining indicating the absence of activated microglia surrounding the observed cell graft. All slides were examined using a conventional bright field microscope and digital pictures were taken under magnification as indicated by the scale bars. (C) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA','shortDescription':'Figure 2 Survival of luciferase-expressing BMSC derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice . (A) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Immunohistochemistry (IHC)','title':'CD11b Antibody (63-0112-82) in IHC','appAbv':'IHC','dateTime':'Jan 7, 2009, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'BMC biotechnology','journalText':'BMC biotechnology 2009 - ','sourceType':'3','benchsci':true,'imageName':'tfs_1457_1472-6750-9-1-2.jpg','benchSciPubmedId':'19128466'},{'sku':'63-0112-82','imageId':'1140890','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29101_gr8.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29101_gr8.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29101_gr8.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29101_gr8.jpg?time\u003d20240507','sortOrder':'79','description':'Adrenergic signaling regulates CD11b expression in myeloid cells and controls migration into the liver after alphaGC treatment (A-E) C57BL/6J WT and Adra2ac -/- mice were treated with alphaGC (2mug/animal), and liver leukocytes were analyzed by flow cytometry after 3 h. Graphs represent the total number of liver leukocytes (CD45 +) (A), neutrophils (CD11b + Ly6G +) (B) and CD11b + F4/80 + cells (C), as well as the CD11b MFI in neutrophils (D) and CD11b + F4/80 + cells (E). (F-J) C57BL6/J WT animals were pre-treated with Yoh (5 mg/kg) or Phe (10 mg/kg) and then treated with alphaGC (2mug/animal). Liver leukocytes were analyzed by flow cytometry after 3 h, and graphs represent the total number of liver leukocytes (CD45 +) (F), neutrophils (CD11b + Ly6G +) (G) and CD11b + F4/80 + cells (H), as well as the CD11b MFI in neutrophils (I) and CD11b + F4/80 + cells (J). Data represent the mean +- SD from a single set of two independent experiments (n \u003d 5). *p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001, ****p \u003c 0.0001.','shortDescription':'Adrenergic signaling regulates CD11b expression in myeloid cells and controls migration into the liver after alphaGC treatment (A-E) C57BL/6J WT and Adra2ac -/- mice were treated with alphaGC (2mug/animal), and liver leukocytes were analyzed by flow cytometry after 3 h. Graphs represent the total number of liver leukocytes (CD45 +) (A), neutrophils (CD11b + Ly6G +) (B) and CD11b + F4/80 + cells (C), as well... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 20, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'iScience','journalText':'iScience 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29101_gr8.jpg','benchSciPubmedId':'37841583'},{'sku':'63-0112-82','imageId':'1140888','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29102_41467_2023_42182_Fig4_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29102_41467_2023_42182_Fig4_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29102_41467_2023_42182_Fig4_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29102_41467_2023_42182_Fig4_HTML.jpg?time\u003d20240507','sortOrder':'80','description':'Terminal maturation of NK cells is associated with the downregulation of Eomes. a - c Newborn C57BL/6 mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. a On day 21 after infection, expression of Eomes and T-bet was determined by flow cytometry. b Eomes expression was analyzed on CD27 + CD11b - , CD27 - CD11b + , KLRG1 + and KLRG1 - NK cells from MCMV and mock-infected mice, representative histograms are shown. c Quantification of Eomes expressing CD27 + CD11b - , CD27 - CD11b + , KLRG1 + and KLRG1 - NK cells ( n \u003d 9 mice per group, Mann-Whitney two-tailed test). Mean values +- SD are shown. d Newborn BALB/c and 129/SvJ mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. On day 21 after infection, mice were euthanized and the frequency of terminally differentiated, KLRG1 and Eomes expressing splenic NK cells was determined ( n \u003d 4 Mock BALB/C, n \u003d 5 MCMV BALB/C, n \u003d 6 Mock 129/SvJ, n \u003d 3 MCMV 129/SvJ, Mann-Whitney two-tailed test). Mean values +- SD are shown. e Newborn C57BL/6 mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. On days 60 and 90 after infection, mice were euthanized and the expression of KLRG1 and Eomes on NK cells was analyzed ( n \u003d 3 Mock 60 d.p.i., n \u003d 4 MCMV 60 d.p.i., n \u003d 4 Mock 90 d.p.i., n \u003d 6 MCMV 90 d.p.i., Mann-Whitney two-tailed test). Mean values +- SD are shown. f - h Adult C56BL/6 mice were infected with 2 x 10 5','shortDescription':'Terminal maturation of NK cells is associated with the downregulation of Eomes. a - c Newborn C57BL/6 mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. a On day 21 after infection, expression of Eomes and T-bet was determined by flow cytometry. b Eomes expression was analyzed on CD27 + CD11b - , CD27 - CD11b + , KLRG1 + and KLRG1 - NK cells from MCMV and mock-in... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 12, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29102_41467_2023_42182_Fig4_HTML.jpg','benchSciPubmedId':'37828009'},{'sku':'63-0112-82','imageId':'1140889','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29102_41467_2023_42182_Fig6_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29102_41467_2023_42182_Fig6_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29102_41467_2023_42182_Fig6_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29102_41467_2023_42182_Fig6_HTML.jpg?time\u003d20240507','sortOrder':'81','description':'Conventional NK cells downregulate Eomes upon perinatal MCMV infection. Newborn mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. a, b On day 21 post-infection of C56BL/6 mice, spleens and livers were harvested. a Expression of CD49b and CD49a was analyzed on NK1.1 + cells, N \u003d 2. b Expression of CD27, CD11b and KLRG1 by ILC1s (CD49b - CD49a + ) was determined by flow cytometry in the liver, N \u003d 2. c On day 21 post-infection of Ncr1 iCre Eomes f/f (KO) or Eomes f/f mice (WT), CD27, CD11b and KLRG1 expression by splenic NK1.1 + cells were determined by flow cytometry. Numbers of terminally differentiated and KLRG1 expressing NK1.1 + cells are shown ( n \u003d 8 WT, n \u003d 6 KO, Mann-Whitney two-tailed test). Mean values +- SD are shown. d On day 21 post-infection of C56BL/6 mice, spleens and bone marrow were harvested. Representative histograms of Eomes expression by NK cells is shown ( N \u003d 3). Statistically significant differences are indicated ( P values). Source data are provided as a Source Data file.','shortDescription':'Conventional NK cells downregulate Eomes upon perinatal MCMV infection. Newborn mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. a, b On day 21 post-infection of C56BL/6 mice, spleens and livers were harvested. a Expression of CD49b and CD49a was analyzed on NK1.1 + cells, N \u003d 2. b Expression of CD27, CD11b and KLRG1 by ILC1s (CD49b - CD49a + ) was determined b... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 12, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29102_41467_2023_42182_Fig6_HTML.jpg','benchSciPubmedId':'37828009'},{'sku':'63-0112-82','imageId':'1140887','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29100_IJN-18-5495-g0006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29100_IJN-18-5495-g0006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29100_IJN-18-5495-g0006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29100_IJN-18-5495-g0006.jpg?time\u003d20240507','sortOrder':'82','description':'Intranasal delivery of EVs in the hydrogel inhibited inflammation. ( A ) Representative flow cytometry plots showing Ly6C high monocyte and Ly6C low monocytes/macrophages (CD45 + CD11b + ) in peripheral blood. and quantification of monocytes/macrophages (Ly6C high or low ) in peripheral blood. (n\u003d5) ( B and C ). ( D ) Representative flow cytometry plots showing neutrophils (CD45 + CD11b + Ly6G + ) in peripheral blood and quantification of neutrophils in peripheral blood. n\u003d5. ( E ). ( F ) Representative images of ventricles staining CD68 (green) showing the infiltration status of macrophages and quantification of CD68 + Cells. (n\u003d5) ( G ). ELISA analysis of inflammatory factors IL-1 ( H ), IL-6 ( I ) and TNF-alpha ( J ) in peripheral blood. (n\u003d5). (*P \u003c 0.05, **P \u003c 0.01, ***P \u003c 0.001). Abbreviation : ns, not significant.','shortDescription':'Intranasal delivery of EVs in the hydrogel inhibited inflammation. ( A ) Representative flow cytometry plots showing Ly6C high monocyte and Ly6C low monocytes/macrophages (CD45 + CD11b + ) in peripheral blood. and quantification of monocytes/macrophages (Ly6C high or low ) in peripheral blood. (n\u003d5) ( B and C ). ( D ) Representative flow cytometry plots showing neutrophils (CD45 + CD11b + Ly6G + ) in periph... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 5, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'International journal of nanomedicine','journalText':'International journal of nanomedicine 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29100_IJN-18-5495-g0006.jpg','benchSciPubmedId':'37791323'},{'sku':'63-0112-82','imageId':'1140886','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29099_fimmu-14-1230681-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29099_fimmu-14-1230681-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29099_fimmu-14-1230681-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29099_fimmu-14-1230681-g003.jpg?time\u003d20240507','sortOrder':'83','description':'Figure 3 P. anaerobius promoted oxaliplatin resistance by recruiting MDSCs into the CRC microenvironment. (A) Viability of MC-38 cells co-cultured with P. anaerobius or in conditioned medium supplemented with PA supernatant (CCK-8 assay, p \u003e 0.05, PA indicated by red arrow). (B) MDSCs (Gr-1 + CD11B + ) from bone marrow of CRC mice detected by multicolor flow cytometry. (C, D) MDSCs infiltrated into the tumor microenvironment detected by fluorescein isothiocyanate (FITC) with anti-mouse CD11b antibody (green), allophycocyanin (APC), anti-mouse Gr-1 antibody (red), and DAPI (blue). (E) Morphological feature of MDSCs collected by FCM (Giemsa staining, purple-blue leaf-shaped or round-type nucleus and almost colorless cytoplasm). (F) Chemotaxis ability of MDSCs treated with PA -CM, MC-38-CM, and PA +MC-38-CM (co-culture medium of PA and MC-38), respectively. (A-F) Data were presented as mean +- SD, p- values were determined by one-way analysis of variance. Three independent experiments were performed with consistent results.','shortDescription':'Figure 3 P. anaerobius promoted oxaliplatin resistance by recruiting MDSCs into the CRC microenvironment. (A) Viability of MC-38 cells co-cultured with P. anaerobius or in conditioned medium supplemented with PA supernatant (CCK-8 assay, p \u003e 0.05, PA indicated by red arrow). (B) MDSCs (Gr-1 + CD11B + ) from bone marrow of CRC mice detected by multicolor flow cytometry. (C, D) MDSCs infiltrated into the tumo... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 3, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29099_fimmu-14-1230681-g003.jpg','benchSciPubmedId':'37781363'},{'sku':'63-0112-82','imageId':'1111335','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29098_fimmu-14-1147695-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29098_fimmu-14-1147695-g005.jpg?time\u003d20240507','sortOrder':'84','description':'Ablation of Cxcr2 in S100a8-expressing cells exacerbates SPEM development. (A) Breeding scheme of S100a8 Cre mice with Cxcr2 flox/flox mice to generate S100a8 Cre Cxcr2 flox/flox mice. (B) RT-qPCR analyses of Cxcr2 expression in FACS-isolated gastric CD11b + Ly6G + cells from 6-month H felis -infected S100a8 Cre Cxcr2 f/f versus Cxcr2 f/f stomachs. (C) Relative stomach weight in milligrams, normalized to total stomach weight in grams, in S100a8 Cre Cxcr2 flox/flox mice versus Cxcr2 flox/flox controls after 6mo H felis infection. (D) Percentage area of SPEM in the gastric mucosae of 6mo H felis -infected S100a8 Cre Cxcr2 flox/flox versus Cxcr2 flox/flox controls. SPEM quantification was performed using 4 longitudinal gastric strips, from the fundus and corpus, two of which were from the lesser and two from the greater curvatures of the stomach, and then by quantifying the percentage of 10x focal planes containing immunofluorescent co-localization of GSII and intrinsic factor, or expansion of GSII and total loss of intrinsic factor-expressing cells. (E) qPCR quantification of H felis flagellar filament B DNA, relative to genomic HPRT DNA, in 6mo H felis -infected S100a8 Cre Cxcr2 flox/flox versus Cxcr2 flox/flox controls. (F) Representative immunofluorescent staining of H + /K + -ATPase (red), GSII (green), and DAPI (blue) in the upper panels, in addition to intrinsic factor (IF, white) in the lower inset panels, in 6mo H felis -infected S100a8 Cre Cxcr2 flox/flox versus Cxcr2','shortDescription':'Ablation of Cxcr2 in S100a8-expressing cells exacerbates SPEM development. (A) Breeding scheme of S100a8 Cre mice with Cxcr2 flox/flox mice to generate S100a8 Cre Cxcr2 flox/flox mice. (B) RT-qPCR analyses of Cxcr2 expression in FACS-isolated gastric CD11b + Ly6G + cells from 6-month H felis -infected S100a8 Cre Cxcr2 f/f versus Cxcr2 f/f stomachs. (C) Relative stomach weight in milligrams, normalized to to... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Sep 29, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29098_fimmu-14-1147695-g005.jpg','benchSciPubmedId':'37744359'},{'sku':'63-0112-82','imageId':'1140884','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29098_fimmu-14-1147695-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29098_fimmu-14-1147695-g001.jpg?time\u003d20240507','sortOrder':'85','description':'Gastric G-MDSCs are characterized by a specific transcriptional signature and can be identified by calprotectin (S100a8 and/or S100a9) expression. (A) Representative FACS plots of gastric MDSC versus non-MDSC myeloid cell populations using CD11b and Ly6G cell-surface protein markers, of dissociated gastric cells from uninfected versus 6mo H felis -infected WT mouse stomachs. Red font \'\'1\'\' annotates gastric non-myeloid and Ly6G-negative cells. Green font \'\'2\'\' annotates CD11b + Ly6G - non-G-MDSC myeloid cells. Black font \'\'3\'\' annotates gastric CD11b + Ly6G + G-MDSC myeloid cells. Blue font \'\'4\'\' annotates gastric CD11b - Ly6G + non-myeloid cells. (B) Microarray heatmap showing the highest expressed genes that are specifically enriched in gastric CD11b + Ly6G - non-G-MDSC myeloid cells (annotated as green font \'\'2\'\'), versus those specifically enriched in gastric CD11b + Ly6G + G-MDSC myeloid cells (annotated as black font \'\'3\'\'), and those specifically enriched in non-myeloid gastric CD11b - Ly6G + control cells (annotated as blue font \'\'4\'\'). The gene names for the calprotectin subunits S100a8 and S100a9, Il1b, Slfn4, and Cxcr2 are listed (S100a8 and Cxcr2 are annotated in red font because they will later be used in the study for Cre/Flox generation). (C) Representative image of the immunohistochemical staining of calprotectin (brown) in 6mo H felis -infected mouse gastric mucosa relative to uninfected.','shortDescription':'Gastric G-MDSCs are characterized by a specific transcriptional signature and can be identified by calprotectin (S100a8 and/or S100a9) expression. (A) Representative FACS plots of gastric MDSC versus non-MDSC myeloid cell populations using CD11b and Ly6G cell-surface protein markers, of dissociated gastric cells from uninfected versus 6mo H felis -infected WT mouse stomachs. Red font \'\'1\'\' annotates gastric... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Sep 29, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29098_fimmu-14-1147695-g001.jpg','benchSciPubmedId':'37744359'},{'sku':'63-0112-82','imageId':'1140885','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29098_fimmu-14-1147695-g006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29098_fimmu-14-1147695-g006.jpg?time\u003d20240507','sortOrder':'86','description':'Cxcr2-deficient S100a8-expressing cells exhibit an altered expression of several genes associated with lipid metabolism, and an increase in the production of the lipid peroxidation product, 4-hydroxynonenal (4-HNE), within gastric epithelial lesions, following 6mo H felis infection. (A) Representative FACS plots of CD11b + Ly6G + G-MDSCs from H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/flox stomachs, which were used for sorting gastric G-MDSCs for microarray analyses. (B) Microarray heatmap of the MDSC-specific genes that exhibited altered expression patterns in G-MDSCs in response to Cre/Flox-mediated Cxcr2 ablation. Fabp5 is directly involved in lipid peroxidation and is annotated by a red arrow. (C) Immunohistochemical staining of 4-HNE staining (brown), within gastric epithelial lesions, in 6mo H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/flox stomachs. (D) Quantitative morphometry of 4-HNE staining. (E) Representative immunohistochemical staining of calprotectin (brown) in 6mo H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/flox stomachs. **P \u003c 0.01.','shortDescription':'Cxcr2-deficient S100a8-expressing cells exhibit an altered expression of several genes associated with lipid metabolism, and an increase in the production of the lipid peroxidation product, 4-hydroxynonenal (4-HNE), within gastric epithelial lesions, following 6mo H felis infection. (A) Representative FACS plots of CD11b + Ly6G + G-MDSCs from H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Sep 29, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29098_fimmu-14-1147695-g006.jpg','benchSciPubmedId':'37744359'},{'sku':'63-0112-82','imageId':'1111144','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29098_fimmu-14-1147695-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29098_fimmu-14-1147695-g002.jpg?time\u003d20240507','sortOrder':'87','description':'Figure 2 S100a8 and S100a9 are enriched in gastric G-MDSCs but not in acutely recruited gut neutrophils. (A) Representative FACS plots of gastric G-MDSCs versus cecal neutrophils in 6-month H felis -infected stomachs versus 2-day C difficile infected ceca respectively. (B) Microarray heatmap showing the highest expressed genes that are specifically enriched in gastric CD11b + Ly6G + G-MDSCs relative to CD11b + Ly6G + cecal neutrophils in 6-month H felis -infected stomachs versus 2-day C difficile infected ceca respectively. The black arrows annotate the genes that are highly expressed in G-MDSCs of 6 month H felis -infected stomachs but not in neutrophils of 2-day C difficile infected ceca.','shortDescription':'Figure 2 S100a8 and S100a9 are enriched in gastric G-MDSCs but not in acutely recruited gut neutrophils. (A) Representative FACS plots of gastric G-MDSCs versus cecal neutrophils in 6-month H felis -infected stomachs versus 2-day C difficile infected ceca respectively. (B) Microarray heatmap showing the highest expressed genes that are specifically enriched in gastric CD11b + Ly6G + G-MDSCs relative to CD11... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Sep 29, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29098_fimmu-14-1147695-g002.jpg','benchSciPubmedId':'37744359'},{'sku':'63-0112-82','imageId':'1140883','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29097_gr3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29097_gr3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29097_gr3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29097_gr3.jpg?time\u003d20240507','sortOrder':'88','description':'Effects of ACSL4 deficiency on PQ-induced lung inflammation. (A) mRNA expression levels of inflammatory cytokines (IL-6 and IL-1beta), COX-2, GPx4, and chemokines (CXCL1, CXCL2, CXCL5, and CXCR2) in lung tissues of untreated or PQ-administered WT (blue columns) and ACSL4-KO mice (red columns); n \u003d 4/group. * p \u003c 0.05 and ** p \u003c 0.01, untreated WT vs PQ-administered WT mice; # p \u003c 0.05 and ## p \u003c 0.01, PQ-administered WT vs ACSL4 KO mice. (B) Representative Gr-1 staining images of lung tissue of untreated or PQ-administered WT and ACSL4-KO mice. The scale bars represent 100 mum. (C) Flow cytometry analysis of CD11b + Ly6G/6C + neutrophils (upper) and CD11 + F4/80+ macrophages (lower) in the BAL fluid cells of untreated or PQ-administered WT and ACSL4-KO mice; cell samples were pooled from 5 mice per group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Fig. 3','shortDescription':'Effects of ACSL4 deficiency on PQ-induced lung inflammation. (A) mRNA expression levels of inflammatory cytokines (IL-6 and IL-1beta), COX-2, GPx4, and chemokines (CXCL1, CXCL2, CXCL5, and CXCR2) in lung tissues of untreated or PQ-administered WT (blue columns) and ACSL4-KO mice (red columns); n \u003d 4/group. * p \u003c 0.05 and ** p \u003c 0.01, untreated WT vs PQ-administered WT mice; # p \u003c 0.05 and ## p \u003c 0.01, PQ-ad... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 1, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Redox biology','journalText':'Redox biology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29097_gr3.jpg','benchSciPubmedId':'37586249'},{'sku':'63-0112-82','imageId':'1140882','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29079_urn-x-wiley-0260437X-media-jat4496-jat4496-fig-0002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29079_urn-x-wiley-0260437X-media-jat4496-jat4496-fig-0002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29079_urn-x-wiley-0260437X-media-jat4496-jat4496-fig-0002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29079_urn-x-wiley-0260437X-media-jat4496-jat4496-fig-0002.jpg?time\u003d20240507','sortOrder':'89','description':'2 FIGURE Flow cytometric analysis of peritoneal macrophages using CD11b-PerCPCy5.5, CD86-PE, and CD206-FITC antibodies after single or dual blocking of toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1) and posttreatment with recombinant interleukin 10 (IL-10). Dot plot images of M1/M2 macrophage population analysis. Graphical representation of the CD86 + M1 population and graphical representation of the CD206 + M2 population in 11 different groups were shown. Data were represented as mean +- SD from three independent experiments. \'\'*\'\' indicates a significant difference in comparison with control in the non-IL-10-treated groups, \'\'#\'\' indicates a significant difference in comparison with lipopolysaccharide (LPS), \'\'*\'\' indicates a significant difference with respect to LPS + DEX, \'\'^\'\' indicates a significant difference with respect to TLR4Ab + LPS, \'\'EUR\'\' indicates significant difference with respect to TNFR1Ab + LPS, \'\'epsilon\'\' indicates significant difference with respect to control in the IL-10-treated group, \'\'delta\'\' indicates a significant difference in comparison with LPS + IL-10, \'\'$\'\' indicates significant difference with respect to LPS + DEX + IL-10, \'\'alpha\'\' indicates significant difference with respect to TLR4Ab + LPS + IL-10, and \'\'U\'\' indicates significant difference with respect to TNFR1Ab + LPS + IL-10. All the differences were statistically significant at p \u003c 0.05 level.','shortDescription':'2 FIGURE Flow cytometric analysis of peritoneal macrophages using CD11b-PerCPCy5.5, CD86-PE, and CD206-FITC antibodies after single or dual blocking of toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1) and posttreatment with recombinant interleukin 10 (IL-10). Dot plot images of M1/M2 macrophage population analysis. Graphical representation of the CD86 + M1 population and graphical re... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Oct 1, 2023, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Journal of applied toxicology : JAT','journalText':'Journal of applied toxicology : JAT 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29079_urn-x-wiley-0260437X-media-jat4496-jat4496-fig-0002.jpg','benchSciPubmedId':'37177863'},{'sku':'63-0112-82','imageId':'1019356','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24743_fphar-13-1095757-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24743_fphar-13-1095757-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24743_fphar-13-1095757-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24743_fphar-13-1095757-g002.jpg?time\u003d20240507','sortOrder':'90','description':'FIGURE 2 Alteration of cellular composition in the diseased status of mice and humans. (A,B) Fractions of major cells types in aneurysmal aorta vs. the corresponding normal aorta of humans (A) and mice (B) . (C) Immunofluorescence analysis for SMMHC (red), alphaSMA (green), and overlays with DAPI-labeled nuclei (blue) showing smooth muscle cells in the human vascular media (scale bars: 100 mum). Lu means the lumen side of the vascular wall, and A means the adventitia side of the vascular wall. (D,E) Immunofluorescence analysis for vimentin (white) (D) and CD45 (purple) (E) and overlays with DAPI-labeled nuclei (blue) showing fibroblasts and immune cells in the human aorta (scale bars: 100 mum). (F) Immunostaining for calponin (red), alphaSMA (green), and overlays with DAPI-labeled nuclei (blue) showing smooth muscle cells in the mouse aortic media (scale bars: 100 mum). (G) Immunostaining for vimentin (white) and DAPI overlay in the thoracic and abdominal aorta form mice subjected to 4 weeks of Ang II induction (scale bars: 500 mum). (H) Immunostaining for CD45 (red), CD68 (green), and overlays with DAPI-labeled nuclei (blue) showing macrophages in the mouse aorta (scale bars: 100 mum). (I) Flow cytometry of the proportion of macrophages (F4/80 + CD11B + ), monocytes (LY6C + CD11B + ), and T cells (CD3 + ) in CD45 + immune cells among three groups (n \u003d 3-4, per group).','shortDescription':'FIGURE 2 Alteration of cellular composition in the diseased status of mice and humans. (A,B) Fractions of major cells types in aneurysmal aorta vs. the corresponding normal aorta of humans (A) and mice (B) . (C) Immunofluorescence analysis for SMMHC (red), alphaSMA (green), and overlays with DAPI-labeled nuclei (blue) showing smooth muscle cells in the human vascular media (scale bars: 100 mum). Lu means th... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Feb 2, 2023, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Frontiers in pharmacology','journalText':'Frontiers in pharmacology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24743_fphar-13-1095757-g002.jpg','benchSciPubmedId':'36703732'},{'sku':'63-0112-82','imageId':'1019336','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24741_12276_2022_851_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24741_12276_2022_851_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24741_12276_2022_851_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24741_12276_2022_851_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'91','description':'Deletion of Cxxc5 ameliorates NASH progression. a - m Cxxc5 +/+ and Cxxc5 -/- mice were fed a HFD for 12 weeks and injected with CCl 4 twice a week for the final 4 weeks to induce NASH ( n \u003d 7 per group). a Representative IHC images of F4/80- and Cd11b-stained liver tissues (left panel). Quantification of hCLS formation and F4/80- and Cd11b-positive areas (right panel). Scale bars, 100 um. b Flow cytometry analyses of the expression of F4/80 and Cd11b (left panel). Percentage of F4/80 + Cd11b + cells ( n \u003d 3) (right panel). c Inflammation score. d Relative mRNA expression levels of genes involved in pro-inflammation ( n \u003d 3). e The NAFLD score. f Representative images of DHE-stained cells (left panel). Quantification of ROS formation by measuring DHE-positive cells in liver tissues (right panel). Scale bar, 100 um. g TUNEL staining of the liver (top panel). Quantification of TUNEL-positive cells (bottom panel). Scale bar, 100 um. h Relative mRNA expression of genes involved in cell death pathways (n \u003d 3). i Representative IHC images for alpha-Sma, collagen I, desmin, and Sirius red staining of liver tissues. Scale bars, 100 um. j Relative mRNA expression of genes involved in fibrogenesis ( n \u003d 3). k The fibrosis score. l , m Plasma concentrations of FFAs ( l ), AST and ALT ( m ). Data represent the mean +- SD. * P \u003c 0.05; ** P \u003c 0.01; *** P \u003c 0.001 determined by Student\u0026apos;s t test.','shortDescription':'Deletion of Cxxc5 ameliorates NASH progression. a - m Cxxc5 +/+ and Cxxc5 -/- mice were fed a HFD for 12 weeks and injected with CCl 4 twice a week for the final 4 weeks to induce NASH ( n \u003d 7 per group). a Representative IHC images of F4/80- and Cd11b-stained liver tissues (left panel). Quantification of hCLS formation and F4/80- and Cd11b-positive areas (right panel). Scale bars, 100 um. b Flow cytometry ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Sep 1, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Experimental \u0026 molecular medicine','journalText':'Experimental \u0026 molecular medicine 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24741_12276_2022_851_Fig3_HTML.jpg','benchSciPubmedId':'36114279'},{'sku':'63-0112-82','imageId':'1019236','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24737_urn-x-wiley-08941491-media-glia24255-glia24255-fig-0001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24737_urn-x-wiley-08941491-media-glia24255-glia24255-fig-0001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24737_urn-x-wiley-08941491-media-glia24255-glia24255-fig-0001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24737_urn-x-wiley-08941491-media-glia24255-glia24255-fig-0001.jpg?time\u003d20240507','sortOrder':'92','description':'1 FIGURE Flow cytometry analysis of the CD11b+ cells. Cells isolated from non-perfused [(a)-(e)] and perfused [(f)-(j)] brains did not show significant differences. [(a), (f)] Histograms showing two CD45-expressing cell populations: CD45 low (microglia) and CD 45 high (possible macrophages). [(b), (g)] Microglia, identified as CD11b+ and CD45 low (Q3), comprised the highest proportion of cells (91.7%-94.5%), whereas a low percentage of potential macrophages (CD11b+/CD45 high, Q2) were observed. [(c), (h)] Forward scatter (FSC) analysis showing a slight smaller size of the CD45 low cells (red) respect to CD45 high (blue) cells. [(d), (i)] CD11b analysis showed a slight lower expression within CD45 low cells (red) respect to CD45 high (blue) cells. [(e), (j)] Forward scatter (FSC) and side scatter (SSC) characteristics of CD11b+/CD45 low (red) and CD11b+/CD45 high (blue) are shown','shortDescription':'1 FIGURE Flow cytometry analysis of the CD11b+ cells. Cells isolated from non-perfused [(a)-(e)] and perfused [(f)-(j)] brains did not show significant differences. [(a), (f)] Histograms showing two CD45-expressing cell populations: CD45 low (microglia) and CD 45 high (possible macrophages). [(b), (g)] Microglia, identified as CD11b+ and CD45 low (Q3), comprised the highest proportion of cells (91.7%-94.5%)... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Dec 1, 2022, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Glia','journalText':'Glia 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24737_urn-x-wiley-08941491-media-glia24255-glia24255-fig-0001.jpg','benchSciPubmedId':'35943203'},{'sku':'63-0112-82','imageId':'952246','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20559_41467_2022_29730_Fig4_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20559_41467_2022_29730_Fig4_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20559_41467_2022_29730_Fig4_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20559_41467_2022_29730_Fig4_HTML.jpg?time\u003d20240507','sortOrder':'93','description':'Fig. 4 CAF-1 depletion induces myeloid differentiation in vivo and a mixed-lineage transcriptional signature in HSPCs. a Schematic of polyI:C (polyinosinic:polycytidylic acid) inducible Mx1-Cre/LoxP system in mice. Flow cytometric analysis of BM MNCs is performed 72 hrs post-induction of mice bearing homozygous Chaf1b floxed alleles and Mx1-Cre ( Chaf1b fl /fl ; Mx1-Cre) and littermate controls ( Chaf1b +/+ ; Mx1-Cre). b Flow cytometric analysis of myeloid differentiation markers (CD11b and Gr1) showing overlaid density plots and histograms of wild-type (Chaf1b +/+ ) and Chaf1b-deleted (Chaf1b Delta/ Delta ) BM MNCs. c Quantification of gated populations in b showing mean and standard deviation of CD11b and Gr1 double-positive and double negative % populations from live BM MNCs isolated from n \u003d 2 wild-type and n \u003d 3 knockout littermates. d Schematic of Chaf1b depletion in primary HSPCs. HSPCs isolated from homozygous floxed mice ( Chaf1b fl /fl ) are transduced with retroviral vectors, either empty control (MIGR1-CTRL) or overexpressing Cre recombinase (MIGR1-Cre) and analyzed by RNA-seq 48 h following expansion in myeloid media conditions. e RNA-seq analysis showing percentages and numbers of upregulated or downregulated genes in CAF-1 OFF iGMPs, HOXA9 OFF iGMPs, and Chaf1b Delta/ Delta HSPCs. DEGs are filtered based on fold change \u003e\u003d 1.5 and FDR \u003c 0.05. f Venn diagrams of unique and common DEGs between CAF-1 OFF iGMPs, HOXA9 OFF iGMPs, and Chaf1b Delta/ Delta HSPCs shown i','shortDescription':'Fig. 4 CAF-1 depletion induces myeloid differentiation in vivo and a mixed-lineage transcriptional signature in HSPCs. a Schematic of polyI:C (polyinosinic:polycytidylic acid) inducible Mx1-Cre/LoxP system in mice. Flow cytometric analysis of BM MNCs is performed 72 hrs post-induction of mice bearing homozygous Chaf1b floxed alleles and Mx1-Cre ( Chaf1b fl /fl ; Mx1-Cre) and littermate controls ( Chaf1b +/+... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Apr 29, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20559_41467_2022_29730_Fig4_HTML.jpg','benchSciPubmedId':'35487911'},{'sku':'63-0112-82','imageId':'952226','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20557_gr5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20557_gr5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20557_gr5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20557_gr5.jpg?time\u003d20240507','sortOrder':'94','description':'The GOG stimulated BMSCs promoted the osteoclastogenesis of HSCs and the inhibited PERK pathway blocked the promotive effects of GOG on osteoblastic/osteoclastic differentiations. (a) The schematic diagram showed the designed induction procedure and scheduled detections at different timepoints. The HSCs were co-cultured with BMSCs in the transwell system for evaluation on the effects of GOG on osteoblastic and osteoclastic differentiations. The HSCs were cultured in the low chamber with 4 days of M-CSF (50 ng/mL) stimulus for inducing BMMs differentiation of HSCs. After 4 days M-CSF induction, the Flow cytometry analysis was conducted to evaluate the proportion of BMMs in total HSCs and the ALP staining was performed to evaluate the osteoblastic differentiation of BMSCs. The osteoclast differentiation medium containing 50 ng/mL M-CSF and 100 ng/mL RANKL was replaced at day 5 in the lower chamber for further analysis on osteoclastic differentiation of BMMs. In the subsequent induction of BMMs, the M1/M2 polarization was detected by M1/M2 specific markers (iNOS and arginase) immunofluorescent staining after 2 days of M-CSF + RANKL induction, and the calcium oscillation of BMMs was also detected after 2 days of M-CSF + RANKL induction. After 6 days of M-CSF + RANKL induction, the mature osteoclast differentiation from BMMs was verified by TRAP staining and the osteoblastic differentiation of BMSCs was evaluated by ARS staining. (b) The FACS analysis on the BMMs differentiation o','shortDescription':'The GOG stimulated BMSCs promoted the osteoclastogenesis of HSCs and the inhibited PERK pathway blocked the promotive effects of GOG on osteoblastic/osteoclastic differentiations. (a) The schematic diagram showed the designed induction procedure and scheduled detections at different timepoints. The HSCs were co-cultured with BMSCs in the transwell system for evaluation on the effects of GOG on osteoblastic ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Sep 1, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Bioactive materials','journalText':'Bioactive materials 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20557_gr5.jpg','benchSciPubmedId':'35386350'},{'sku':'63-0112-82','imageId':'1019316','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24740_41467_2022_29471_Fig9_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24740_41467_2022_29471_Fig9_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24740_41467_2022_29471_Fig9_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24740_41467_2022_29471_Fig9_HTML.jpg?time\u003d20240507','sortOrder':'95','description':'Pharmacological inhibitor of MLCK MW01-022AZ suppressed tumor inflammation and growth. a Schematic of LLC lung tumor implantation in C57Bl6 mice and MW01-022AZ treatment. b-d Effect of MW01-022AZ on LLC b gross tumor morphology ( n \u003d tumors from 6 mice) c tumor volume ( n \u003d 9 mice and d tumor weight at endpoint (21 days) ( n \u003d 9 mice). *p \u003c 0.0001 for vehicle vs MW01-022AZ treatment, determined by t -test. e FACS profiles and f quantification of Gr1-CD11b+ ( p \u003d 0.1146) Gr1loCD11b+ (* p \u003d 0.0402), Gr1hiCD11b+ (* p \u003d 0.0146) myeloid cells in representative MW01-022AZ vs vehicle-treated tumors at endpoint (21 days) ( n \u003d 3 mice), determined by t -test. For Facs gating scheme, see Supplementary Fig. 7b . g Quantification and h representative histographs of F4/80+ macrophages ( n \u003d sections from 6 mice) *p \u003c 0.0001 for vehicle or MW01-022AZ by t -test, CD31+ blood vessels ( n \u003d sections from 7 mice) *p \u003c 0.0001 for vehicle or MW01-022AZ by t -test, CD8+ T cells ( n \u003d sections from 9 mice) *p \u003d 0.0244 for vehicle vs MW01-022AZ by Mann-Whitney test in tissues from mice treated with vehicle or MW01-022AZ . i Model of integrin activation demonstrating that cytokine stimulation activates a pathway whereby PI3Kgamma induces MLCK210 and myosin activation and colocalization with Rap1, resulting in Rap1-mediated integrin alpha4 conformation changes and interaction of the integrin with the actin cytoskeleton. Data are presented as mean values +- SEM. Statistical significance determined by','shortDescription':'Pharmacological inhibitor of MLCK MW01-022AZ suppressed tumor inflammation and growth. a Schematic of LLC lung tumor implantation in C57Bl6 mice and MW01-022AZ treatment. b-d Effect of MW01-022AZ on LLC b gross tumor morphology ( n \u003d tumors from 6 mice) c tumor volume ( n \u003d 9 mice and d tumor weight at endpoint (21 days) ( n \u003d 9 mice). *p \u003c 0.0001 for vehicle vs MW01-022AZ treatment, determined by t -test. ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Apr 1, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24740_41467_2022_29471_Fig9_HTML.jpg','benchSciPubmedId':'35365657'},{'sku':'63-0112-82','imageId':'1019296','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_24740_41467_2022_29471_Fig7_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_24740_41467_2022_29471_Fig7_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_24740_41467_2022_29471_Fig7_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_24740_41467_2022_29471_Fig7_HTML.jpg?time\u003d20240507','sortOrder':'96','description':'Deletion of bone marrow cell MLCK210 inhibits tumor growth. a Schematic of bone marrow transplantation and tumor implantation. b Genomic PCR to detect Mlck 210 gene in peripheral blood cells from BMT transplanted mice ( n \u003d 2 mice). c - e Tumor volumes ( c ), weights at endpoint (15 days) (*p \u003c 0.0001 WT vs Mlck210 -/- BM) ( d ) and images of gross tumor morphology ( e ) from LLC tumors in WT mice transplanted with bone marrow from WT ( n \u003d 10 mice) or Mlck210 -/- mice ( n \u003d 10 mice). f Representative FACS profiles and g quantification of Gr1-CD11b+ ( p \u003d 0.9724) Gr1loCD11b+ (*p \u003d 0.0280), Gr1hiCD11b+ ( p \u003d 0.8641) myeloid cells in representative WT vs Mlck210 -/- BM transplanted tumors harvested at endpoint (15 days) ( n \u003d 3 mice). For Facs gating scheme, see Supplementary Fig. 7b . h Representative histographs and i quantification of F4/80+ macrophages/field ( n \u003d sections from 5 WT mice and 5 Mlck210 -/- ) *p \u003d 0.0009, CD31+ blood vessels/field ( n \u003d sections from 5 WT and 3 Mlck210 -/- mice) *p \u003d 0.001, CD8+ T cells/field ( n \u003d sections from 5 WT and 4 Mlck210 -/- mice) *p \u003d 0.019 in tumor sections and 100X H\u0026E-stained tumor sections to detect percent necrosis/field ( n \u003d sections from 5 mice) *p \u003d 0.0004 from mice transplanted with WT vs Mlck210 -/- bone marrow. Data are presented as mean values +- SEM. Significance testing was performed by Student\u0026apos;s t -test. Experiments were performed twice. Source data are provided with this article as a Source Data file.','shortDescription':'Deletion of bone marrow cell MLCK210 inhibits tumor growth. a Schematic of bone marrow transplantation and tumor implantation. b Genomic PCR to detect Mlck 210 gene in peripheral blood cells from BMT transplanted mice ( n \u003d 2 mice). c - e Tumor volumes ( c ), weights at endpoint (15 days) (*p \u003c 0.0001 WT vs Mlck210 -/- BM) ( d ) and images of gross tumor morphology ( e ) from LLC tumors in WT mice transplan... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Apr 1, 2022, 4:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_24740_41467_2022_29471_Fig7_HTML.jpg','benchSciPubmedId':'35365657'},{'sku':'63-0112-82','imageId':'952205','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20556_pntd.0010224.g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20556_pntd.0010224.g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20556_pntd.0010224.g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20556_pntd.0010224.g002.jpg?time\u003d20240507','sortOrder':'97','description':'10.1371/journal.pntd.0010224.g002 Fig 2 During LdCen -/- ID infection, neutrophil derived increased CCL3 recruits significantly higher number of dendritic cells attributed with proinflammatory phenotype in the ear dLN compared to LdWT infection. (A) Parasitized neutrophils (Cd11b + Ly6G + Ly6c int RFP/mCherry + ) were flow sorted from the ear dLN 5d post infection and mRNA expression levels of CCL3 from sorted neutrophils in the ear dLN were estimated by qPCR as described in Materials and Methods and expressed as fold change from uninfected naive mice. The experiment was repeated three times with pooled digests from five to six ear dLNs per experiment. The data represent the mean values +- SD of results from three independent experiments that all yielded similar results (n \u003d 5). ** p \u003c 0.005 between the groups. (B) The gating strategy and individual flow plots for DCs (CD11b + Ly6G - Ly6C - CD11c + MHCII hi ) recruitment in ear dLNs 5d post infection is shown under CCL3 non-depleted or depleted condition. (C ) Total number of Cd11b + cells recruited in ear dLN 5 days post infection in mice depleted or not of CCL3 has been shown. (D) Changes in the total number of DCs per ear dLN. Values shown are the mean numbers of cells per ear dLN +- SD of results. 6-8 ear dLN, pooled data from 3 independent experiments that all yielded similar results (n \u003d 6). *p\u003c 0.05; ** p \u003c 0.005, *** p \u003c 0.0005 between the groups. ( E) Parasitized DCs (CD11b + CD11c + MHCII hi RFP/mCherry + ) were flo','shortDescription':'10.1371/journal.pntd.0010224.g002 Fig 2 During LdCen -/- ID infection, neutrophil derived increased CCL3 recruits significantly higher number of dendritic cells attributed with proinflammatory phenotype in the ear dLN compared to LdWT infection. (A) Parasitized neutrophils (Cd11b + Ly6G + Ly6c int RFP/mCherry + ) were flow sorted from the ear dLN 5d post infection and mRNA expression levels of CCL3 from sor... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Feb 1, 2022, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'PLoS neglected tropical diseases','journalText':'PLoS neglected tropical diseases 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20556_pntd.0010224.g002.jpg','benchSciPubmedId':'35192633'},{'sku':'63-0112-82','imageId':'1019276','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19070_41467_2021_27683_Fig9_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19070_41467_2021_27683_Fig9_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19070_41467_2021_27683_Fig9_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19070_41467_2021_27683_Fig9_HTML.jpg?time\u003d20240507','sortOrder':'98','description':'Fig. 9 OPN induces lysosomal-dependent lipid metabolism in BMDMs. a Representative western blots (left) and quantification (right, n \u003d 3 biologically independent samples) of OPN receptor alphav and beta3 expression in BMDMs and metabolically activated macrophages (MMes) after culture for 4 days in vitro. b Schematic diagram illustrating the co-culture system as indicated below ( c ). c , d Representative images ( c ) and quantification ( n \u003d 5 biologically independent samples) ( d ) of crystal violet staining of BMDMs (ctrl) and MMes treated with or without rOPN for 24 h in a Transwell system. Scale bar: 50 mum. e Immunofluorescence images of buoyant BMDMs (F4/80) containing lipid vesicles (Perilipin 1) isolated from bone marrow supernatant at week 12. Scale bar: 15 mum. f FACS analysis of FC + (F4/80+ and CD11b+ , top) and Perilipin1+ (bottom) BMDMs from NFD- (left) and HFD-fed (right) mice. P9, FC + and Perilipin1+ BMDM population from Q2. g Percentage of FC + and Perilipin1+ BMDMs ( n \u003d 5 biologically independent samples). h Lactate concentration in bone marrow supernatant ( n \u003d 5 biologically independent samples). i Schematic diagram illustrating the co-culture system as described below ( j ). j Immunofluorescence staining of the co-cultured BMDMs for 4 days in vitro; the eWAT from mice fed the HFD for 8 weeks was placed in the upper chamber; Neu Ab (2.0 mug/ml) was added into the lower chamber. Scale bar: 50 mum. The right-most image is an enlarged view of the field in t','shortDescription':'Fig. 9 OPN induces lysosomal-dependent lipid metabolism in BMDMs. a Representative western blots (left) and quantification (right, n \u003d 3 biologically independent samples) of OPN receptor alphav and beta3 expression in BMDMs and metabolically activated macrophages (MMes) after culture for 4 days in vitro. b Schematic diagram illustrating the co-culture system as indicated below ( c ). c , d Representative im... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Jan 20, 2022, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19070_41467_2021_27683_Fig9_HTML.jpg','benchSciPubmedId':'35058428'},{'sku':'63-0112-82','imageId':'926419','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19070_41467_2021_27683_Fig5_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19070_41467_2021_27683_Fig5_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19070_41467_2021_27683_Fig5_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19070_41467_2021_27683_Fig5_HTML.jpg?time\u003d20240507','sortOrder':'99','description':'Fig. 5 OPN is primarily secreted by F4/80 + and CD11b+ macrophages in eWAT. a Representative images of immunofluorescence staining of OPN in eWAT from NFD- and HFD-fed mice at weeks 8, 12, and 16. Scale bar: 100 mum. b Quantification of the F4/80-positive or OPN-positive stained area percentage in eWAT from a ( n \u003d 4 biologically independent samples). c Relative expression of Spp1 in the adipocyte fraction (AF) and stromal vascular fraction (SVF) from eWAT or iWAT of HFD-fed mice at week 12 ( n \u003d 3 biologically independent samples). d ATMs gated by FACS for F4/80 and CD11b expression from eWAT at week 12 of NFD (left) or HFD feeding (right). Q2 represents the F4/80 + CD11b + (FC + ) ATM population, and Q3 represents the F4/80-CD11b- (FC-) ATM population. e Percentage of FC + ATMs from NFD- and HFD-fed mice at week 12 ( n \u003d 4 biologically independent samples). f Relative expression of Spp1 in FC+ and FC- ATMs from HFD-fed mice at week 12 ( n \u003d 3 biologically independent samples). Images are representative of 3 independent experiments. All data are presented as mean +- SD. Two-way ANOVA with Sidak\u0027s post-hoc test ( b ) and two-tailed Welch\u0027s t -test ( c , e , f ) were used. Source data are provided as a Source Data file.','shortDescription':'Fig. 5 OPN is primarily secreted by F4/80 + and CD11b+ macrophages in eWAT. a Representative images of immunofluorescence staining of OPN in eWAT from NFD- and HFD-fed mice at weeks 8, 12, and 16. Scale bar: 100 mum. b Quantification of the F4/80-positive or OPN-positive stained area percentage in eWAT from a ( n \u003d 4 biologically independent samples). c Relative expression of Spp1 in the adipocyte fraction ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD11b Antibody in Flow Cytometry (Flow)','title':'CD11b Antibody (63-0112-82) in Flow','appAbv':'Flow','dateTime':'Jan 20, 2022, 5:00:00 AM','appName':'Flow Cytometry','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19070_41467_2021_27683_Fig5_HTML.jpg','benchSciPubmedId':'35058428'}],'Advanced Verification':[]}" image-displayed-id="'121560'" product-sub-type="'antibody_primary'" prod-type="'antibody'" page-type="PDP" product-id="'63-0112-82'"> <div class="tabs"> <ul class="nav nav-pills"> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Antibody Testing Data"> <a class="tab-text"> Antibody Testing Data (1) </a> </li> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Published Figures"> <a class="tab-text"> Published Figures (93) </a> </li> </ul> <div gallery-flt class="gal-flt" is-with-abbr="true" enable-mobile-flt="true" mobile-result-count="true" filter-data="$ctrl.applicationsList" advanced-filter-data="$ctrl.advancedApplicationsList" filter-text="Application" event-name="{{$ctrl.fltAppEvent}}"> </div> </div> <div class="cntnt" ng-swipe-right="$ctrl.clickLeftArrow()" ng-swipe-left="$ctrl.clickRightArrow()"> <div class="nav-arrow" ng-if="!$ctrl.isMobileSmallTabletScreen()" onclick="window.THERMO_FISHER.inp.onClickOfLeftArrow(event, this)"> <div tabindex="0" class="gallery-left-arrows icon-gallery-arrow-left" > </div> </div> <div class="media-text"> <div class="media-display-mobile" ng-if="$ctrl.isMobileSmallTabletScreen()"> <div class="nav-arrow" onclick="window.THERMO_FISHER.inp.onClickOfLeftArrow(event, this)"> <div tabindex="0" class="gallery-left-arrows icon-gallery-arrow-left" > </div> </div> <div class="media-display"> <img id="media-image" class="image" ng-show="!$ctrl.isVideo" src="https://www.thermofisher.com/antibody/images/650/63-0112-82-CD11b-Flow-20170216130641.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody in Flow Cytometry (Flow)" longdesc="https://www.thermofisher.com/antibody/images/650/63-0112-82-CD11b-Flow-20170216130641.jpg?time=20240507?time=20240507" onerror="javascript:imageErrorHandler.call(this, event);" > <div class="hover-text" ng-show="!$ctrl.isVideo"> <a href="javascript:return false;" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)" aria-label="image-link"> <div class="rectangle"> <div class="expand-logo-rightcorner"> <?xml version="1.0" encoding="utf-8"?> <!-- Generator: Adobe Illustrator 23.0.4, SVG Export Plug-In . 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Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="952246" data-index="87"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_20559_41467_2022_29730_Fig4_HTML.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="952226" data-index="88"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_20557_gr5.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="1019316" data-index="89"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_24740_41467_2022_29471_Fig9_HTML.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="1019296" data-index="90"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_24740_41467_2022_29471_Fig7_HTML.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="952205" data-index="91"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_20556_pntd.0010224.g002.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="1019276" data-index="92"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_19070_41467_2021_27683_Fig9_HTML.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="926419" data-index="93"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_19070_41467_2021_27683_Fig5_HTML.jpg?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)"/> </div> </div> </div> </div> </div> <noscript> <div itemscope itemtype="http://schema.org/ImageGallery"> <h2>Product Image Gallery</h2> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/63-0112-82-CD11b-Flow-20170216130641.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control Super Bright 600 (Product # 63-4031-82) (left) or 0.06 µg of Anti-Mouse CD11b Super Bright 600 (right). Cells in the large scatter population were used for analysis.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24742_biology-11-01609-g004.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Figure 4 TIM3-positive macrophages were recruited to ATC xenograft tumors. ( A ) I. Upper panel: TIM3 mRNA expression in RAW264.7 cells and in ATC xenografts. Total RNA was isolated, and the mRNA expression of TIM3 was examined by RT-PCR analysis. Lane 1, RAW264.7 cells; lanes 2-4, 8505C xenograft tumors ( n = 3); lanes 5-7, C643 xenograft tumors ( n = 3). The lower panel represents the expression of actin. II. TIM3 mRNA expression was normalized by beta-actin. Densitometry analysis of TIM3 is shown. Representative results of 3 independent experiments. ( B - D ) 20X and 63X images of 5 um thick 8505C xenograft tumor sections immunostained with TIM3-PE antibody (red). Nuclei were counterstained with DAPI. Representative immunofluorescence images of 3 independent experiments. ( E - G ) Representative FACS analysis of 2 independent experiments of macrophages (CD11b + F4/80 + TIM3 + ) out of all CD45 + cells in the 8505c xenograft tumors ( E ) and C643 xenograft tumors ( F ). ( G ) Quantification of the percentage of TIM3 + cells ( n = 6-8).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24738_urn-x-wiley-26882663-media-mco2193-mco2193-fig-0004.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">4 FIGURE Deciphering the diversity of myeloid cells in infected lung lesions. (A) Uniform manifold approximation and projection (UMAP) plot of the defined myeloid cell subsets in the six samples. (B) UMAP plot of the myeloid cell subsets in the three groups. (C) Bar plot showing the relative proportions of identified cell populations in each sample, colored according to cluster designation. Identified cell types are shown on the right. (D) Dotplot of the top expressed genes distinctly within the subclusters of myeloid cells. Avg. Exp: average expression; % of Exp: percent of expression. (E) Immunofluorescence (IF) of neutrophils with 4',6-diamidino-2-phenylindole (DAPI) (blue) and myeloperoxidase (MPO) (red) colored among the three groups is shown. Magnification: 20x. Scale bar = 100 mum. (F and G) Multiplex immunohistochemistry (mIHC) showing coexpression of CD11c (green), Siglec-F (red), and DAPI (blue)-positive cells called alveolar macrophages (AMs) (F) and CD11b (green), Mertk (red), and DAPI (blue)-positive cells called interstitial macrophages (IMs) (G) among the three groups. Magnification: 20x. Scale bar = 100 mum. (H-J) Representative flow cytometry plots and quantification showing the proportional changes in neutrophils (H), AMs (I), and IMs (J) in the three groups. (K) Histograms showing the proportion of neutrophils, AMs and IMs among the three groups ( n = 3). Bars represent mean +- SD. Significant differences were designated by using analysis of variance (</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24742_biology-11-01609-g003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Figure 3 M2-like macrophages were recruited to ATC xenograft tumors. ( A , B ) Representative FACS analysis of macrophages (CD11b + F4/80 + ) out of all CD45 + cells infiltrating 8505C xenograft tumors ( A ) and C643 xenograft tumors ( B ). ( C ) Quantification of the percentage of CD11b + F4/80 + cells ( n = 8-10). ( D , F ) Representative FACS analysis of M2-macrophages (CD11b + F4/80 + CD206 + ) out of all CD45 + cells infiltrating 8505C xenograft tumors ( D ) and C643 xenograft tumors ( E ). ( F ) Quantification of the percentage of CD11b + F4/80 + CD206 + cells ( n = 3-5). ( G , H ) Representative histograms of Arg1 ( G ) and iNOS ( H ) expression in CD206 low/int (blue histograms) and CD206 high (pink histograms) populations in total cells from C643 xenograft tumors. ( I ) Mean fluorescence intensity (MFI) of Arg1/iNOS ratio in the CD206 high group and in the CD206 low/int group ( n = 8-10). Data are expressed as mean +- SEM of 3 independent experiments. ** p < 0.005.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14985_pone.0255296.g003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">10.1371/journal.pone.0255296.g003 Fig 3 Chronodisruption causes increased macrophage and microglial signaling in the placenta. Placentas from ( A-C ) control light (CL) and mice exposed to ( D-F ) developmental chronodisruption (CD) were labeled for inflammatory markers labeling microglia and macrophages within the decidual zone. In placenta from CD mice increased placental ( G ) Iba1 fluorescence (Student's 2-tailed unpaired t-test, t = 2.49, df = 8, p = 0.038) and increased placental (H ) CD11b fluorescence (Student's 2-tailed unpaired t-test, t = 2.70, df = 8, p = 0.027) were detected. CL = 4 placenta from different dams, 3 images each; CD = 6 placenta from different dams, 3 images each. All data are presented as mean +- SEM, scale bar = 20 microns, and * = p<0.05.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14991_fcvm-08-649124-g0003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))" title="CD11b Antibody (63-0112-82) in ICC/IF, IHC (PFA)"> <figcaption itemprop="caption">Figure 3 Histochemical analysis of eye cross-sections show reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. (A) Histochemical analysis of eye cross-sections shows reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. Enlarged images of the corneal region of two mice visibly show more swelling and a higher presence of inflammatory cells in the control mice. Inflammatory cell nuclei are stained by the deep-purple spots marked with white triangles in the light pink stromal layer of the cornea. (B) To further identify infiltrating immune cells, the slides were stained with CD11b (an immune cell marker, green). (C) CD11b positive cells (cells/0.1 mm 2 ) were quantified ( n = 3/group; * p < 0.05).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14925_gr8.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 8 Absence of IDO contributed to increased CD8 + T infiltration in colon. ( A ) Absolute numbers of CD4 + and CD8 + T cells infiltrated in tumor were measured by flow cytometry (n = 6). ( B ) The percentage of CD4 + and CD8 + T cells in spleen lymphocytes was analyzed by flow cytometry (n = 6). ( C , D ) Absolute numbers of CD4 + and CD8 + T cells in spleen lymphocytes were detected by flow cytometry (n = 6). ( E , F ) The frequency of intratumoral CD8 + / CD69 + T cells was determined (n = 6). ( G , H ) The percentage of CD8 + GZB + T cells was detected (n = 6). ( I , J ) The expression of Ki67 and its mean fluorescence intensity (MFI) of CD8 + T were shown (n = 6). ( K , L ) Annexin V expression on intratumoral CD8 + T cells and its mean fluorescence intensity were determined by flow cytometry (n = 6). ( M , N ) Serial sections of colon tissues were immunostained with DAPI (blue), anti F4/80 (green), and CD8 (red). They were observed by confocal laser scanning microscope (scale bar = 50 mum, 10 mum). Expressions of CD11b (red) and CD4 (green) were analyzed by immunofluorescence cytochemistry (scale bar = 50 mum, 10 mum). The results are representative of at least 6 independent experiments and expressed as mean +- SD. * P < .05, ** P < .01 indicates significant difference.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14977_41467_2021_23197_Fig1_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Fig. 1 Generation and characterization of a genetic system for specific targeting of cavity macrophages. a Schematic figure showing CD45-Dre mediates Stop cassette removal from Gata6-iCreER and places CreER directly under Gata6 promoter. After tamoxifen (Tam), Cre-loxP recombination labels cells by tdTomato. b Intersectional genetics marks CD45 + GATA6 + cells as tdTomato + . c Schematic figure showing experimental design using cavity macrophage tracing tool CD45-Dre;Gata6-iCreER ( G6Mo-CreER ). d Flow cytometric analysis of the percentage of tdTomato + cells in macrophages from blood, pleural, and peritoneal cavity with or without Tam. e Immunostaining for tdTomato, CD11b, GATA6, F4/80 on dissociated cells from blood, pleural, or peritoneal cavity. Boxed regions are magnified. f Quantification of the percentage of tdTomato + cells in GATA6 + or F4/80 + macrophages. Data are the mean +- SD; n = 5 mice per group. g Quantification of the percentage of GATA6 + or F4/80 + macrophages in tdTomato + cells. Data are the mean +- SD; n = 5 mice per group. h FACS showing the percentage of tdTomato + cells expressing F4/80. i Whole-mount epifluorescence images and immunostaining for CD45 and tdTomato shows no resident tdTomato + macrophages in visceral organs. Each image is representative of 5 individual biological samples. Scale bars, yellow, 1 mm; white, 100 um. Source data are provided as a Source Data file.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0008.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">LRRK2 inhibits the fast recycling of receptors internalized through macropinocytosis A-D Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male WT-mLRRK2 BAC transgenic mice were immunostained with anti-mouse CCR5, or CD11b, or MHC II antibody (shown as green signal) and (A) total Rab10 antibody (red signal), or (B) pT73-Rab10 antibody (red signal) as indicated. Representative photomicrographs (from > 20 images analyzed for each condition from n = 3 biologically independent experiments) are shown. (C) Photomicrographs showing the different cargo with respect to Rab10 tubular structures. (D) EEA1 (shown as green signal) with total Rab10 or pT73-Rab10 (shown as red signals) as indicated. Bounding boxes in panels A-D are magnified in ""High Mag"" panels that show individual vesicles. Scale bars represent 10 and 2 mum for ""High Mag"" images. E Calculated percentage of vesicles positive with tubular structures ( n > 30 vesicles analyzed from cells from n = 3 biologically independent experiments) are shown. Each dot represents one vesicle, with significance assessed by Mann-Whitney test. ** represents P < 0.01. Error bars represent +- SEM. F BMDM cells were treated with 0.004% DMSO or 100 nM MLi2 prior to live cell surface staining with Alexa-594 conjugated rat anti-mouse CD11b antibody on ice for 10 min. Three wells of cells from each condition were fixed and imaged for initial surface CD11b binding (shown in red as indicated). Six wells of cells from each cond</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9847_urn-x-wiley-02614189-media-embj2020104862-embj2020104862-fig-0004ev.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">EV4 LRRK2 phosphorylates Rab10 on vesicular structures A Raw264.7 cells were transfected with eGFP(N-term)-WT Rab10 (show as green signal) and treated with 0.001% DMSO or 100 nM MLi2 (i.e., ~IC 90 concentration) for 2 h prior to fixation and immunostaining with pT73-Rab10 antibody (shown as red signal). Representative photomicrographs (from > 20 images analyzed for each condition from n = 3 biologically independent experiments) are shown. Scale bars indicate 20 mum. B Raw264.7 cells were transfected with eGFP(N-term)-Rab10 before fixation and analyzed with the pT73-Rab10 antibody. Z-stack images of Rab10 transfected cells were collected using Airyscan microscopy. Orthogonal views of representative images are shown. Red dashed line is the intersection of y,z and yellow dashed line is x,z. Blue box is the x,y plane, red box is the y,z plane, and yellow box is the x,z plane. Scale bar represents 10 mum. C Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male Ntg or WT-mLRRK2 BAC transgenic mice were treated with DMSO or 100 nM MLi2 for 2 h. Representative immunoblots of cells derived from three different animals for each genotype are shown. D, E Relative LRRK2 or pT73-Rab10 levels were calculated as fold changes from Ntg cells (male C57BL/6J) treated with DMSO (0.004% to match DMSO levels with MLi2 treatment). Data are calculated from three independent replicates, with each dot in the column graph represents one independent experiment. Error bars represent +-</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/nihms948434f10-52-20190221105219.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Extended data Figure 6 Premarking in ESCs is required for robust future macrophage enhancer activation a, Photomicrographs of macrophages differentiated from ESCs documented by F4/80 and CD11b staining. Scale bar=50 uM. b , Screenshot of the Tlr1 locus. The blue box corresponds to CRISPR/Cas9 target region. c, d, The putative Tlr1 enhancer mutant sequence diagram and DNA sequence documentation of several of the homozygous mutation clones, used for analysis. e, Screenshot of the Tnfaip3 locus. The blue box corresponds to CRISPR/Cas9 target region. f, g, The putative Tnfaip3 enhancer mutant sequence diagram and DNA sequence documentation of several of the homozygous mutation clones, used for analysis. h, Tlr1 eRNA transcription was tested in wt and mutant clonal cells (#26, #45) of Tnfaip3 enhancer. One representative experiment is plotted (n=2 biological repeats). i, UCSC genome browser shot image of RNA-seq in Tlr1 locus in normal ESCs and clonal mutant ESCs (wt, #10, #14). Primer sets are listed in Supplementary Table 2 .</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/nihms865773f3-1-20190103084817.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Extended Data Figure 3 Wnt ligands produced predominantly by cancer cells drive activation of the Wnt signaling pathway in lung adenocarcinoma a , Schematic representation of the lentivirus vector 46 used to transduce a Kras G12D/+ ; Trp53Delta/Delta ; Rosa26 tdTomato/+ ( KPT ) LUAD cell line, followed by puromycin selection. b , tdTomato and 7TCF::Luciferase signals at baseline (0h) and 48h following treatment with 10 mg/kg/d LGK974 or vehicle. Red arrows indicate two subcutaneous tumours with reduced 7TCF-dependent bioluminescence in response to 48h of LGK974 treatment. c , Suppression of 7TCF-driven bioluminescence by LGK974 relative to tdTomato signal in mice harboring subcutaneous transplants of the KPT LUAD cell line stably expressing the 7TCF::Luciferase-PGK-Puro lentivirus. N = 6 tumours, 3 mice per group. Representative data from two independent experiments. Student's two-sided t -test; error bars: +-SD. d , Haematoxylin-eosin (HE) or beta-catenin staining in KP adenomas or in adenocarcinomas. Scale bars: 100 mum. e , Immunofluorescence for beta-catenin (red) and Porcupine (green) in a Kras G12D/+ ; Trp53Delta/Delta ( KP ) lung adenocarcinoma (LUAD). Scale bar: 100 mum. f , In situ hybridization (ISH) for Axin2 mRNA (purple, arrowheads) in KP lung adenomas or adenocarcinomas. Scale bar: 50 mum. g , Immunofluorescence for Porcupine (green, white arrowheads) and ISH for Axin2 (red, yellow arrowheads) in KP LUAD. Scale bar: 10 mum. h , Immunofluorescence for Porcupine (</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_3278_srep36659-f7.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)" title="CD11b Antibody (63-0112-82) in ICC/IF, IHC"> <figcaption itemprop="caption">Figure 7 Macrophages form a perfused vascular network in tumors. ( A-D ) Images represent individual laser channels of a 38 mum Z-stack representing an A375 human melanoma subcutaneous tumor in an athymic mouse. Tumor cells were injected in the absence of matrigel and tumor perfusion was assessed by administering a 3 KD rhodamine dextran by tail vein injection prior to tumor removal. Tumor was harvested, fixed and immunostained with anti-mouse antibodies as follows: ( A ) Hoechst nuclear stain (blue), ( B ) CD11b (purple), ( C ) CD31 (green) and ( D ) iv 3 KD rhodamine dextran (red). ( E ) Merged image comprised of the four fluorescent channels presented in ( A-D ). Note that most of the perfused structures are strongly positive for CD11b. ( F-H ) Magnified view of individual laser channels in the area highlighted by the white box in ( A-D ) shows that the intravenously injected dye localizes to the CD11b + tubular structure (blue arrow) which is not CD31 + . ( I ) Imaris 3D cross sectional rendering of the CD11b + vessels depicted by the dashed white line in image ( E ) shows the dye to be within the lumen of the CD11b + channel. ( J-N ) CD11b + tubes are connected to multinucleated, tuft-like structures which express CD31, CD11b and are perfused by systemically injected dye. ( J ) Low power view of a rosette-like structure within the tumor associated macrophage VM network. Circled area shows an Imaris rendered tumor tuft ( K-N ) that is connected to the predominately CD11b</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27158241_IF_12_gr4_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Fig. 4 Ginsenoside Rd reduces M1 macrophage in situ infiltration in IRI tissue detected by immunofluorescence staining. (A-C)Red and green fluorescence indicates CD11b + , iNOS + /tumor necrosis factor-alpha + /interleukin-12 + macrophages, respectively. Blue fluorescence indicates total nuclei in renal tissue. Original magnification: 400x. (D-F) Corresponding percentage of M1 macrophage among all kidney cells. (Eight randomly selected fields/slice, n = 6 mice for each group, values represent mean +- SD in the histograms, * p < 0.05 vs vehicle.)</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646012_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 12 Fibronectin and type I collagen (collagen I) in the cells in the fragments of mouse splenic granulomas. The scale bars are ((a, right panel), (c, right panel)) 20 mu m each and ((a)-(e)) 10 mu m each. ((b), (d)-(e)) Colocalization of the markers on confocal images of cells (yellow signal). Nuclei stained by DAPI (blue signal). ((a), (c)) Immunochemical localization of (a) fibronectin and (c) type I collagen in granuloma cells and control mouse peritoneal macrophages (Mph) and splenic fibroblasts (Fibr). The brown color indicates the presence of the markers in these cells. The black arrows indicate fibroblasts; the other cells are macrophages, dendritic cells, and lymphocytes. (b) Confocal immunofluorescent localization of fibronectin (green signal) and CD11b (red signal) in granuloma macrophages. In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. A macrophage with BCG-mycobacteria reproducing in it is indicated by the white arrows on the fluorescent images and the black arrow on the ZN image. (d) Confocal immunofluorescent localization of type I collagen (green signal) and CD11b (red signal) in a granuloma macrophage with BCG-mycobacteria. In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. (e) Confocal immunofluorescent localization of fibronectin (green signal), type I collagen (green signal), and filamentous actin (red signal) i</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646007_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 7 ((a)-(c)) Bacterial lipid- and glycolipid-presenting molecule CD1d in the cells in the fragments of mouse splenic granulomas. The scale bars are ((a, central right panel), (b)-(d)) 10 mu m each and (a, other panels) 20 mu m each. ((b)-(c)) Colocalization of the markers on the confocal images of cells (yellow signal). ((b)-(d)) Nuclei stained by DAPI (blue signal). (a) Immunochemical localization of CD1d in granuloma cells, control mouse peritoneal macrophages (Mph), and fibroblasts (Fibr). The brown color indicates the presence of CD1d in these cells. ((b)-(c)) Confocal immunofluorescent localization of CD1d (green signal) and IL-1 alpha (red signal) in granuloma macrophages and fibroblasts. (b) CD1d- and IL-1 alpha -producing fibroblasts are indicated by the white arrows on the fluorescent images and the black arrows on the 3D image. (c) In the right panel, the same fragment as in the left panel restained for acid-fast BCG-mycobacteria by the ZN method. A CD1d- and IL-1 alpha -producing macrophage with BCG-mycobacteria reproducing in it is indicated by the white arrow on the fluorescent image and the black arrow on the ZN image. (d) Confocal immunofluorescent localization of CD1d (red signal) in the cells in the cultures of bone marrow macrophages following infection with the BCG vaccine in vitro and after culture for several hours. The macrophage with CD1d in it is indicated by the red arrow, and a megakaryocyte without the antigen is indicated by the white arrow.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646006_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 6 ((a)-(c)) The cells with IFN gamma in the fragments of mouse splenic granulomas. The scale bars are 10 mu m each. ((a), (c)) Colocalization of the markers on the confocal images of cells (yellow signal). ((a), (c)-(d)) Nuclei stained by DAPI (blue signal). (a) Confocal immunofluorescent localization of IFN gamma (green signal) and CD80 (red signal) in granuloma macrophages (Mph) and a fibroblast at the center of the images. In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. An IFN gamma -producing macrophage with BCG-mycobacteria reproducing in it is indicated by the white arrows on the fluorescent images and the black arrow on the ZN image. (b) Immunochemical localization of IFN gamma in granuloma cells and control mouse peritoneal macrophages. The brown color indicates the presence of IFN gamma in these cells. (c) Confocal immunofluorescent localization of IFN gamma (green signal) and CD11b (red signal) in granuloma macrophages, with a multinucleate Langhans giant cell indicated by the white arrows in the three lower panels. (d) Bone marrow macrophages following infection with the BCG vaccine in vitro and after culture for several hours not stained by the IFN gamma - (green signal) and IL-1 alpha -specific antibodies (red signal) on the confocal fluorescent images. Abbreviations as in Figure 1 .</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646014_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 14 The S100 proteins in the cells in the fragments of mouse splenic granulomas and control mouse peritoneal macrophages (Mph). The scale bars are 10 mu m each. In the left panel, confocal immunofluorescent localization of S100 proteins (green signal) and CD11b (red signal). Colocalization of the markers on confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the central and right panels, immunochemical localization of S100 proteins in the cells. The brown color indicates the presence of the markers in these cells. The black and red arrows indicate macrophages and a dendritic cell, respectively, with increased production of S100 proteins. Abbreviations as in Figure 1 .</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27066505_IF_12_JIR2016-4325646013_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 13 Confocal immunofluorescent localization of vimentin (green signal) and CD11b (red signal) in the cells in a fragment of a mouse splenic granuloma. The scale bars are 10 mu m each. Colocalization of the markers on the confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. Macrophages with BCG-mycobacteria reproducing in them are indicated by the white arrows on the fluorescent images and the black arrows on the ZN image. Abbreviations as in Figure 1 .</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_26656646_IF_12_nihms776849f2_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14946_urn-x-wiley-00219541-media-jcp25239-jcp25239-fig-0003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">FLCs are different from macrophages. (A) Cell morphology of mouse FLCs at passage 2 (right panel) and macrophage cell line Raw 264.7 (left panel). (B) FLCs at passage 1 and Raw264.7 were stained with either CD11b or CD11c or CD14 antibody. Scale bars, 50 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/14011282-26275291-CD11b-pone0133298g002.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Fig 2 Virulent Yersinia becomes partially embedded within the macrophage plasma membrane. (A) RAW macrophages were infected with YPIII/pYV for 100 minutes and analyzed by scanning electron microscopy (SEM). Shown is a representative image depicting a single macrophage infected with several YPIII/pYV bacteria. Shown in the enlargements are bacteria partially enveloped within host membrane. The bacterium in panel b is indicated by an arrow. Shown below is a false coloring rendering highlighting the demarcation of the host cell (blue) and YPIII/pYV bacteria (red). (B) Macrophages were infected with YPIII/pYV for 100 minutes and then stained for the plasma membrane marker (CD11b), the early endosomal marker (EEA1), and a lysosomal marker (LAMP1) and analyzed by fluorescent microscopy. The arrow in the enlarged image of the CD11b-stained cells indicates a bacterium closely associated with host cell membrane; 62% of macrophage-associated bacteria (N = 56) were similarly observed. Shown in the right panels of the EEA1- and LAMP1-stained cells is the percentage of bacteria-containing endosomes that stained for either EEA1 or LAMP1 (N = 111 and 52, respectively). The arrow in the enlarged image indicates a bacterium distinct from an EEA1-staining compartment. The data shown is representative of 3 independent experiments with similar results using both RAW macrophages and murine peritoneal macrophages.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_26275291_IF_13_pone0133298s003_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/16011282-26080408-CD11b-pnas1424355112sfig04.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_24463523_IF_12_nihms543080f3_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 3 AM-MyD88 in inflammatory signaling ( a-c ) Synchronized (Syn) and non-synchronized (Non) Ca 2+ spikes (arrows) and oscillations in wild type (WT, n = 12), CD11cMyD88 -/- (My -/- , n = 5) and CD11cCx43 -/- (Cx -/- , n = 5) mice. ( d ) Alveoli (green), and Ly6G + (red) and CD11b + neutrophils (blue) 24 h after LPS. Scale bar, 30 mum. n = 4 ( e ) Responses are 24 h after treatments. LPS WT: n = 9, others: n = 4. Bars, mean+-SEM. *P<0.05.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_24446236_IF_12_nihms562085f7_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_22361049_IF_13_1742-2094-9-39-5_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunocytochemistry (ICC/IF)" title="CD11b Antibody (63-0112-82) in ICC/IF"> <figcaption itemprop="caption">Figure 5 Microglial activation in whole-mount retina and microglial migration in an in vitro model . ( A ) Microglia in wild-type (WT) retina have a different shape from those in the trif -/- group as shown by CD11b immunostaining at 7 and 14 days post-crush (7dPC and 14dPC). Scale bar = 20 mum. ( B ) Estimated numbers of microglia/mm 2 in whole-mount retinas were different between different time-course groups (* P < 0.05 increase relative to sham and 14dPC group). ( C ) Estimated number per mm 2 of microglia with an amoeboid shape in whole-mount retinas were different between wild-type (WT and trif -/- groups at 7 and 14 days post-crush (7dPC and 14dPC). * P < 0.01 increase relative to WT group in 7dPC and 14dPC. ( D ) Outline of whole-mount retina. Square frames (red) were counted using double-blinded quantification. ( E ) In a transwell system, microglia and retinal ganglion cells (RGCs) were cultured together (as shown in drawing). Microglia became activated and migrated to the other side of the membrane when stimulated by injured RGCs in the lower well. Stained with cresyl violet acetate. Scale bar = 20 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14904_biology-10-00472-g005.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))" title="CD11b Antibody (63-0112-82) in IHC (F)"> <figcaption itemprop="caption">Figure 5 Expression of IL-16 by CNS resident cells in EAE mice. Spinal cord and brain tissues were harvested from EAE and PBS control mice and stained with IL-16 (green) together with NeuN, GFAP or CD11b (all red). Representative images of spinal cord ( A - C ) and hippocampus ( D ) tissues are shown. Dotted white lines indicate tissue section edges. White arrows indicate areas of colocalisation. Percentage of double-positive cells in each group within either the spinal cord ( A - C ) or hippocampus ( E - G ) and cerebellum ( H - J ) was quantified with results expressed as the mean +- SEM. N = 5. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001 versus PBS or comparison groups are indicated by the bar lines.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12625_iovs-62-4-22-f008.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))" title="CD11b Antibody (63-0112-82) in IHC (F)"> <figcaption itemprop="caption">Figure 8. Both CD and HFD cause increased retinal immune activation, as measured by CD11b ( green ) and Iba1 ( red ). Representative retinal immunofluorescence microscopy results, with ( left ) and without ( right ) DAPI staining ( blue ), showing retinal layers of the ( A ) CL + CON group, the ( B ) CL + HFD group, the ( C ) CD + CON group, and the ( D ) CD + HFD group show increased retinal expression of CD11b in CL + HFD, CD + CON, and CD + HFD groups and increased retinal expression of Iba1 in the CD + CON and CD + HFD groups. Measured fluorescence by retinal layer, showing no difference in ( E ) CD11b staining in the OPL (1-way ANOVA, F(3, 17) = 1.23, P = 0.33) but increased Iba1 staining in the OPL (1-way ANOVA, F(3, 17) = 6.25, P = 0.0047) in the CD + HFD group. ( F ) In the INL, there was also no difference in CD11b expression (1-way ANOVA, F(3, 17) = 2.52, P = 0.092), but Iba1 expression was increased (1-way ANOVA, F(3, 17) = 5.36, P = 0.022) in the CD + CON and CD + HFD groups. In the IPL, ( G ) CD11b increased (1-way ANOVA, F(3, 17) = 8.06, P = 0.0015) in both HFD groups while Iba1 increased (1-way ANOVA, F(3, 17) = 15.87, P < 0.0001) in both CD groups. Likewise, in the GCL, ( H ) CD11b was increased (1-way ANOVA, F(3, 17) = 4.34, P = 0.019) in the CL + HFD group and Iba1 was increased (1-way ANOVA, F(3, 17) = 3.89, P = 0.023) in both CD groups. ( I ) Drawing representing the retinal layers. Images from the CL + CON group ( n = 5 mice), CL + HFD group ( n = 4 mice)</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_1457_1472-6750-9-1-3.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))" title="CD11b Antibody (63-0112-82) in IHC, IHC (F)"> <figcaption itemprop="caption">Figure 3 Survival of BMSC genetically modified with multiple reporter genes following implantation in the central nervous system of syngeneic immunocompetent mice . (A) Histogram overlay showing a representative flow cytometric analysis of eGFP expression by BMSC expressing the luciferase-, eGFP- and puromycin resistance genes (BMSC-Luc/eGFP/Pac, filled histogram). Parental BMSC were used as negative control (open histogram). (B) In vitro luminescence assay on parental BMSC and on clonal BMSC-Luc/eGFP/Pac. (C) In vivo real time bioluminescence imaging (BLI) of clonal BMSC-Luc/eGFP/Pac grafts in the central nervous system (CNS) of syngeneic immunocompetent mice at week 2 post-implantation. (D) In vivo real time BLI of clonal BMSC-Luc/eGFP/Pac intramuscular grafts in syngeneic immunocompetent mice at week 2 post-injection. (E) Representative histological analysis of clonal BMSC-Luc/eGFP/Pac implants in the CNS of syngeneic immunocompetent mice at week 2 post-implantation. Left picture: haematoxylin-eosin (HE) staining showing general appearance of the cell implantation site. Middle picture: direct eGFP-fluorescence indicating the BMSC-Luc/eGFP/Pac origin of the observed cell implant. Right picture: CD11b staining indicating a limited number of microglia surrounding the observed cell graft. All slides were examined using a conventional bright field microscope and digital pictures were taken under magnification as indicated by the scale bars.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12626_JIR-14-1789-g0004.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 4 Rb1 mitigates inflammation in Ang II-infused mice. C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 6.25 mg/kg (Rb1-L), 25 mg/kg (Rb1-M) and 100 mg/kg (Rb1-H). ( A ) Plasma was isolated and analyze by ELISA for the level of IL-1 beta, IL-6 and TNF (n=6-8 per group). ( B ) Immunohistochemistry (IHC) was performed to examine the presence of CD68 + cells (in red) in the heart. DAPI (in blue) was counterstained to help visualize the nuclei. Scale bar, 50 mum. ( C ) The number of CD68 + cells in the heart was quantified (n=5 per group). ( D ) IHC was performed to reveal the presence of CD11b + cells (in red) in the heart. DAPI (in blue) counterstaining was performed to visualize the nuclei. Scale bar, 50 mum. ( E ) The number of CD11b + cells in the heart was quantified (n=5 per group). ( F) . C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 100 mg/kg (Rb1). The cardiac expression of Il1b, II6 , and Tnf was analyzed by real-time qPCR (n=6-7 per group). 18S rRNA was included as the internal control. Relative fold change was plotted against that from the sham controls. Data were expressed as mean+-S.E.M. * P<0.05, ** P<0.01, *** P<0.001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12624_ijms-22-03452-g003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 3 CD11b- and TLR-2-activated alveolar macrophages with an increased expression of the NF- kappa B p50 and p65 subunits in them are detected on the histological sections obtained from the lung tissues distant from tuberculomas (d), but not from tuberculoma walls (t) for TB patients 24-29. ( A ) The number of alveolar macrophages with the markers expressed as the percentage of the total number of the TB patients'' alveolar macrophages analyzed. The expression of the markers is shown as in Figure 2 A. Red arrows indicate a trend in the alteration of the number of the markers-positive alveolar macrophages between various lung lesions of the same TB patients. ( B - D ) Representative confocal fluorescent images of cells stained by antibodies to human ( B ) CD14 (green signal) and TLR-2 (red signal), ( C ) NF- kappa B p50 (red signal), ( D ) NF- kappa B p65 (green signal), and CD11b (red signal) demonstrate colocalization of some markers (yellow signal). Nuclei are stained by DAPI (blue signal). Close-ups of the parts of these images with alveolar macrophages and other cells, with or without the examined markers in them, are shown in the corners. The scale bars are 50 um for the histological sections and 10 um for the enlarged views of them. ( C , D ) The NF- kappa B subunits localize in the cytoplasm, but not in the nuclei of alveolar macrophages. White arrows point to alveolar macrophages used to create the profile images. Red arrows identify the areas for the construction</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9857_41419_2020_3223_Fig3_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)" title="CD11b Antibody (63-0112-82) in IHC, Flow"> <figcaption itemprop="caption">Fig. 3 ZD55-IL-24 inhibits melanoma growth in B16-bearing immunocompetent mouse model mainly through the indirect pathway of inducing systemic antitumor immunity. A , B Changes of the immune-related cytokines in tumors treated with ZD55-IL-24. A Heat-map of the down-regulated immunosuppressive cytokines in Fig. 2 C . B Heat-map of the up-regulated immunostimulatory cytokines in Fig. 2 D . C - J Immunohistochemical analysis of immune cell infiltration in tumors, as well as recruitment and activation in spleens. Tumors and spleens resected from B16-bearing C57BL/6 mice receiving PBS or ZD55-IL-24 treatment indicated in Fig. 1 A were analyzed 2 days after the last injection by immunohistochemical staining. C Shown are representative images of tumor sections immunostained for CD8 (red, left panel), CD11b and Ly-6G (yellow, middle panel), and NK1.1 (red, right panel). D Quantification of the CD8 + cells in C ( n = 11). E Quantification of the CD11b + Ly-6G + Ly-6C low neutrophils in C ( n = 3). f Quantification of the NK1.1 + cells in c ( n = 6). G Quantification of the CD8 + cells in H ( n = 9). H Shown are representative images of spleen sections immunostained for CD8 (red, left panel), CD11b and Ly-6G (yellow, middle panel), and NK1.1 (red, right panel). I Quantification of the CD11b + Ly-6G + Ly-6C low neutrophils in H ( n = 3). j Quantification of NK1.1 + cells in h ( n = 9). Nuclei is counterstained with DAPI (blue). Data are mean +- SD. Scale bars, 300 um. Shown is one of t</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9856_cancers-12-03457-g002.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 2 Transcriptomic profiling of pre-metastatic lungs demonstrates an inflammatory response and myeloid cell infiltration. ( A ) Volcano plot of differentially expressed genes in pre-metastatic lungs compared to control lungs. Dots colored in red or blue represent upregulated and downregulated genes, respectively, meeting the thresholds of fold change >1.5, p -value < 0.01, and FDR <0.2 respectively. ( B ) Heatmap representation of upregulated genes under the GO term ""inflammatory response"" (GO:0006954) in tumor-bearing mice vs. control mice. Each column represents one biological replicate ( n = 6 in the tumor group (T1-T6), n = 4 in the control group (PBS1-PBS4)). Cut-off values of fold change are >1.5 and FDR <0.2. The clustering separates the genes by color with positive or negative row-scaled Z-scores represented in red and blue, respectively. ( C ) Representative immunofluorescence images (left panel) and quantification (right panel) of CD11b + cells (red) and nuclei (blue) in lungs of control mice (day 12 and day 28) and tumor-bearing mice (day 12 and day 28), respectively. Scale bar, 500 um. (n.s., non-significant, *, p < 0.05, ***, p = 0.001; Bonferroni''s one-way ANOVA test).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9856_cancers-12-03457-g003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)" title="CD11b Antibody (63-0112-82) in IHC, Flow"> <figcaption itemprop="caption">Figure 3 Osteosarcoma cell-derived extracellular-vesicles (EVs) promote accumulation of bone marrow-derived cells in the lungs. ( A ) Schematic illustration of the experimental setup. Mice were treated with either PBS, 143-B EVs or SAOS-2 EVs (10 ug) 2 times a week for 3 weeks after which mice were euthanized. ( B ) Relative mRNA expression levels of integrin alpha M gene encoding CD11b ( Itgam ) in bulk lung RNA in mice of indicated treatment groups. Transcript levels are normalized to Gapdh and expressed as fold change relative to control mice. n = 6, mice were pooled from two independent experiments indicated in red and black ( C ) Representative flow cytometric profile (left panel) and quantification (right panel) of CD11b + cells isolated from lungs of mice from indicated treatment groups; n = 3 mice per group. ( D ) Representative immunofluorescence images (left panel) and quantification (right panel) of CD11b + cells (red) and nuclei (blue) in lungs of mice from indicated treatment groups. Scale bar, 500 um. (*, p < 0.05, **, p < 0.01, ***, p = 0.0001, ****, p < 0.0001, Bonferroni''s one-way ANOVA test).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9855_cells-09-02344-g005.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))" title="CD11b Antibody (63-0112-82) in IHC, IHC (PFA)"> <figcaption itemprop="caption">Figure 5 Immunofluorescent analysis of Lrrk2 distribution and subcellular localization in mBAC-Lrrk2-G2019S brain. Representative co-immunofluorescent staining of brain slices from hemizygous transgenic mice overexpressing murine BAC-Lrrk2-G2019S. In detail: ( a , b ) tot-Lrrk2 (green) distribution in cortex and striatum and subcellular localization in beta-III-tubulin positive neurons (red) of 1-month-old and 12-months-old mice; ( c , d ) tot-Lrrk2 (red) distribution in cortex and striatum and subcellular localization in GLT-1 positive astrocytes (green) of 1-month-old and 12-months-old mice; ( e , f ) tot-Lrrk2 (red) distribution in cortex and striatum and subcellular localization in CD11b positive microglia (green) of 1-month-old and 12-months-old mice. Representative immunofluorescent staining of ( g , h ) GFAP and ( i , j ) CD11b-positive cells highlighting the different shape and activation level of striatal astrocytes and microglia respectively, at 1 and 12 months of age. n = 3 animals each staining. Scale bars 200 mum, and 50 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14972_cancers-12-03072-g006.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 6 IL-15 GET induces immune cell infiltrate into the tumor microenvironment. Haematoxylin and eosin stain. Anti-CD3e (green-T cells), anti-CD11b (green-APCs), and anti-CD335 (green--natural killer cells) staining (green) reveal the presence of immune cells within the tumors of mice treated using EP2 to deliver pAG115, p,G170, and pAG208 on days 0, 4, and 7. Tissue was collected on day 8 (24 h after the third treatment). Images are the best representative sample of n = 2 for each group. Sections were counterstained with a nuclear dye 4',6-diamidino-2-phenylindole (DAPI) (Blue). Mag = 20x. Scale bar = 500 um.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19066_db191239f2.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 2 Adipocyte Becn1 deficiency promotes adipose tissue inflammation. A : Histological analysis of eWAT from 10-week-old WT and AKO mice. WAT sections were examined by H&E staining. Black arrows indicate CLSs. Scale bars, 50 um. B : Quantification of CLSs in WT and AKO mice ( n = 8). C : The mRNA expression levels of F4/80 , Cd11b , and Cd11c in eWAT from 10-week-old WT and AKO mice ( n = 7). D : Representative immunofluorescence images of eWAT from 10-week-old WT and AKO mice. The eWAT sections were stained with CD11b (cyan) and CD11c (red) antibodies. Nucleus was stained with DAPI (blue). Scale bars, 75 um. E : The mRNA expression of M1 and M2 macrophage-specific markers in eWAT from 10-week-old WT and AKO mice ( n = 7). F : Total number of F4/80 + CD11b + SVCs per gram of eWAT was determined by flow cytometry in WT and AKO mice ( n = 3). G : The percentages of CD11c + and CD11c - CD206 + in F4/80 + CD11b + cells were measured by flow cytometry in the SVCs of WT and AKO mice ( n = 3). The qRT-PCR results were normalized to Ppia . Data are presented as mean +- SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; # P = 0.12. exp., expression; FM, fat mass.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24324_12FF3.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">FIGURE 3. Navitoclax alleviates the inflammatory reaction induced by Ang II. A, Representative images of immunofluorescent staining of CD11B (red) and DAPI (blue), which show inflammatory cells of heart sections and quantification of the CD11B fluorescent area among the different groups. B, Representative western blotting bands of adhesion molecules and cytokine levels in the heart tissue of the mouse model and quantitative analysis of relative densities compared with GAPDH (fold). C, Protein expression of ICAM1, VCAM1, IL-6, and TNF-&agr in vitro and quantitative analysis of relative densities compared with &bgr-actin (fold). Scale bars = 50 &mgrm. &ast P < 0.05, &ast&ast P < 0.01. All data in the bar graphs are shown as mean +- SEM with 4 mice per group. Navi, navitoclax.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9850_pbio.3000734.s009.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))" title="CD11b Antibody (63-0112-82) in IHC, IHC (PFA)"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry and Immunohistochemistry (PFA fixed)</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19065_nihms-1546634-f0008.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Extended Data Fig. 3 | Stress-induced hair greying is not mediated through corticosterone or immune attack. a, Left, white hair formation after RTX injection in Rag1 mutant mice devoid of T and B cells (Rag1 KO, n = 6 for each condition, two-tailed unpaired t -test). Right, immunofluorescent staining for T cell marker CD3 (green) in control and Rag1 KO skin (n = 6 mice for each condition, two-tailed unpaired t -test). b, Left, hair greying occurs when RTX is injected into CD11b-DTR mice treated with diphtheria toxin (DT) to deplete myeloid cells (n = 6 mice for each condition). Right, immunofluorescent staining for CD11b (green) in DT treated control and CD11b-DTR skin (n = 6 mice for each condition). c, Expression of adrenergic receptors and glucocorticoid receptor (GR) in MeSCs (n = 2 biologically independent samples). d, White hair formation following RTX injection into Tyr-CreER; GR fl/fl mice (MeSC-GR cKO; n = 6 mice for each condition, two-tailed unpaired t -test). e, Left, enzyme-linked immunosorbent assay (ELISA) measurement of corticosterone level in the blood 3 days after supplying corticosterone in drinking water (n = 4 mice for each condition). Middle, immunofluorescent staining of hair follicles for TRP2 (red) from mice 5 days after corticosterone treatment (n = 30 HFs from 3 mice for each condition, two-tailed unpaired t -test). Right, hair coat colour after HFs in corticosterone-treated mice enter another round of anagen to regenerate new hairs. CORT: corticost</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/enu0051827770006-29-20190221105219.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 6. Microglia expressing pro-/anti-inflammatory markers. Representative confocal immunofluorescent microscopy of CD11b (red) and pro-/anti-inflammatory markers (green) within the lumbar spinal cord are shown (images are from a WT B6 mouse on day 1 post-L5Tx surgery). Merged panels show colocalization of CD11b with the corresponding inflammatory marker. Scale bar: 40 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_5940_jciinsight-3-99722-g084.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g4.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 4. Nrf2/HO-1/Gp91phox expression and Nrf2/HO-1/Gp91phox microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. Nrf2/HO-1/Gp91phox expression in brain was assayed by Western blot. A) Representative bands of Nrf2 immunostaining. B) Quantitative data of Nrf2 immunostaining. Nrf2/HO-1/Gp91phox positive microglia in brain was assayed by double immunohistochemistry of Nrf2/HO-1/Gp91phox and CD11b. C) Representative images of Nrf2 expression on CD11b + microglia. D) Quantitative data of double positive cells. E) Representative bands of HO-1 immunostaining. F) quantitative data of HO-1 immunostaining; G) Representative images of HO-1 expression on CD11b + microglia. H) Quantitative data of double positive cells; I) Representative bands of gp91phox immunostaining. J) Quantitative data of gp91phox immunostaining. K) Representative images of gp91phox expression on CD11b + microglia. L) Quantitative data of double positive cells. Quantitative results are mean +- SEM of 4 mice in each group. * p <0.05, ** p <0.01, ***p<0.001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g3.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 3. The levels of TNF-alpha, IL-1beta, iNOS/Arg-1 expression, and iNOS/Arg-1 microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. The levels of TNF-alpha and IL-1beta in brain were assayed by ELISA. A) TNF-alpha; and B) IL-1beta expression in brain was assayed by Western blot. C) Representative bands of iNOS immunostaining. D) Quantitative data of iNOS immunostaining. iNOS/Arg-1 positive microglia in brain was assayed by double immunohistochemistry of iNOS/Arg-1 and CD11b. E) Representative images of iNOS expression on CD11b + microglia. F) Quantitative data of double positive cells. G) Representative bands of Arg-1 immunostaining. H) Quantitative data of Arg-1 immunostaining. I) Representative images of Arg-1 expression on CD11b + microglia. J) Quantitative data of double positive cells. Quantitative results are mean +- SEM of 4 mice in each group. * p <0.05, ** p <0.01.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_4791_ad-9-5-785-g2.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 2. TLR2/p-NF-kappaB/p65 expression and TLR2/p-NF-kappaB/p65 microglia in brain of young and aged mice stimulated with or without LPS Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. TLR2/p-NF-kappaB/p65 expression in brain was assayed by Western blot. A) Representative bands of TLR2 immunostaining. B) Quantitative data of TLR2 immunostaining. TLR2/p-NF-kappaB/p65 positive microglia in brain was assayed by double immunohistochemistry of TLR2/p-NF-kappaB/p65 and CD11b. C) Representative images of TLR2 expression on CD11b + microglia. D) Quantitative data of double positive cells. E) Representative bands of p-NF-kappaB/p65 immunostaining. F) Quantitative data of p-NF-kappaB/p65 immunostaining. G) Representative images of p-NF-kappaB/p65 expression on CD11b + microglia. H) Quantitative data of double positive cells. Quantitative results are mean +- SEM of 4 mice in each group. * p <0.05, ** p <0.01.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/12974_2018_1207_Fig2_HTML-44-20190221105219.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Fig. 2 Vascular congestion in IL-10 KO mice with malaria includes thrombi-containing monocytes and T cells. Immunofluorescent staining of fixed, frozen brain sections (30 mum) from P. chabaudi -infected IL-10 KO mice (day 8 p.i., n = 4 mice). a Confocal images (x 40) of IL-10 KO brain stained for fibrin (red). b Successive single-plane confocal images (x 40) of a 30-mum z-stack showing complete occlusion of a large vessel with residual fibrinogen (red). c Immunofluorescence staining of IL-10 KO brains showing fibrin staining of blood vessels (red) and leukocytes expressing CD45 (green, x 60) and d CD11b (green, x 40). e CTV + CD4 T cells (blue) from infected IL-10 KO mice were adoptively transferred into infection-matched IL-10 KO (day 7 p.i.) recipients 3.5 h before sacrifice. Frozen brain sections (day 7 p.i.) were stained for fibrin (red). Max intensity projection of a 30-mum z-stack (x 240) displayed from brain tissue of IL-10 KO mice co-stained with WT control samples ( n = 3-4 mice per group). Scale bars represent 30 mum ( a ), 50 mum ( b - d ), or 10 mum ( d )</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_5918_pnas.1722020115fig03.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)" title="CD11b Antibody (63-0112-82) in IHC, Flow"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry and Flow Cytometry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12614_nihms950531f3.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_4189_fphys-09-00047-g0013.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6068_12974_2017_990_Fig2_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Fig. 2 Intracerebroventricular (ICV) administration of IL-1beta induces NF-kappaB nuclear localization in a time- and region-dependent manner. The effect of ICV IL-1beta is both rapid and transient as demonstrated by NF-kappaB immunoreactivity (IR) in representative confocal images of the paraventricular nucleus (PVN). IR in vehicle (aCSF, n = 6)-treated animals showed cytoplasmic labeling of blood vessels ( a ). IL-1beta treatment caused NF-kappaB nuclear localization that peaked by 30 min ( b , n = 8), persisted for at least 2 h ( c , n = 4) and returned to near baseline, with cytoplasmic vascular patterns reappearing, by 4 h after treatment ( d , n = 4). This effect was most prominent in and around the PVN, organum vasculosum lamina terminalis (OVLT; e , f ), subfornical organ (SFO; g , h ), arcuate nucleus/median eminence (ARC/ME; i , j ), and choroid plexus (ChP; k , l ). High-magnification confocal images of co-labeling with cell-specific markers shows that vascular endothelial cells ( m ), microglia ( n ), astrocytes ( o ), and tanycytes ( p ) directly respond to IL-1beta. Scale bars: a - l = 50 mum, m - p = 10 mum</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14956_kaup-13-12-1380757-g003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12605_41598_2017_8535_Fig1_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 1 Upregulation of HDAC3 in microglia/macrophages after SCI. ( a ) Representative sagittal immunohistochemistry images and quantification demonstrate upregulation of HDAC3 (green) at lesion center 7 days post injury (7 dpi) by T8 dorsal column transection (SCI-T). DAPI nuclear counterstaining highlights the increased cellularity at lesion core. Enlarged images of boxed areas are shown on the right. Quantification of the number of HDAC3+ cells at the injury site is shown on the right: *p = 0.015, parametric slit-plot ANOVA. 3 pairs of mice were examined, with 3 representative images quantified for each animal. ( b ) Representative immunohistochemistry images of HDAC3 at different time points after SCI-T as compared to sham. ( c ) Representative immunofluorescent images of co-immunolabeling show a large overlap of HDAC3+ cells (green) and CD11b+ or Iba1+ immune cells (red) at the injury site at 7 days after SCI-T. Enlarged images of boxed areas are shown on the right. ( d ) Immunohistochemistry images for CD11b (red) and different class I HDACs (green) at 7 days after SCI-T. No significant overlaps were detected between these HDACs and CD11b. Orientation: R: Rostral, C: Caudal, D: Dorsal, V: Ventral. Scale bars: 100 um ( a , b , c ), 25 um ( d , enlarged images in a , c ).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_3470_oncotarget-08-95-g008.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 8 Berberine increased neutrophil recruitment in the peritoneal cavity and the colon C57BL/6 mice were administered (i.g.) with berberine (100 mg/kg body weight) or vehicle (2% Tween-80 in PBS) once a day for 3 consecutive days prior to injection (i.p.) with viable E. coli (2x10 9 CFU/mouse). After indicated time periods, cells in the peritoneal cavity were harvested by 1.5 ml PBS and double stained with FITC-CD11b and PerCP-Gr-1 antibodies for flow cytometric analysis, and the colon was collected and fixed in 4% neutral formaldehyde for histological analysis. A. Representative flow cytometric dot-plots of three independent experiments. B. Quantification of CD11b + Gr-1 + cells (neutrophils) within the gate in A. Data are expressed as mean +- SD ( n = 3). * P < 0.05; *** P < 0.001; ns, not significant. C. Hematoxylin and eosin (H&E) staining of the colon sections 8 h after infection ( n = 3). Representative images from each mouse are shown. Arrows indicate infiltrated inflammatory cells in the colon tissues. Scale bar, 100 mum; BBR, berberine.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_27840922_IF_13_MMR-14-06-4947-g04_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 5. RI inhibited microglia activation in mice with MPTP-induced parkinsonism. (A) ROCKII-positive and CD11b-positive staining is increased in the SN in mice with MPTP-induced PD compared with the control mice (magnification x20). The majority of ROCKII-positive cells were CD11b-positive. (B) CD11b-positive staining declined markedly in the RI mice as ROCKII-positive staining declined (magnification, x20). ROCKII, Rho/Rho-associated, coiled-coil-containing protein kinase II; RI, ROCKII interference; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; PD, Parkinson's disease; CD11b, cluster of differentiation 11b; SN, substantia nigra.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/11011282-27065324-CD11b-nihms852440f3.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_2970_dmm-9-022475-g1.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)" title="CD11b Antibody (63-0112-82) in IHC, Flow"> <figcaption itemprop="caption">Fig. 1. Flow cytometry can quantify the leukocyte infiltrate during EIU and be used as a scoring system for identifying therapeutic effects. In the absence of disease, (A) the anterior chamber is unremarkable and (B) negligible numbers of CD45+CD11b+Ly6G+ cells are detected by flow cytometry of whole ocular tissues. Numbers within the graphs in B indicate the percentage of each parent gate. At 18 h following intravitreal delivery of LPS, (C) marked ocular infiltration occurs throughout the eye, with leukocyte sediment (hypopyon) seen in the anterior chamber (indicated by the red arrow). (D) Representative example of the gating strategy to identify CD45+CD11b+Ly6G+ and Ly6C+ cells. (E) Immunohistochemistry targeting the same epitopes as the flow cytometry antibodies confirms the presence of leukocytes in the anterior chamber (AC) and vitreous, staining for (F) CD45 and (G) CD11b. CE, ciliary epithelium. (H) Ly6G+ cells (magenta) are polymorphonuclear, consistent with neutrophils, whereas Ly6C+ cells (white) appear mononuclear, consistent with monocytes. Scale bars: 20 mum. (I) Compared with untreated eyes, a statistically significant elevation in the number of neutrophils (CD45+CD11b+Ly6G+) can be seen during EIU and is proposed as a single measure score. Mann-Whitney U -test, n =20. (J) Using multicolour flow cytometry, the average cellular composition of the infiltrate in EIU was determined. Mean values from eight eyes undergoing standard processing. See Fig. S2 for the gati</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/13011282-26658452-CD11b-NIHMS727001-supplement-2.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12590_13058_2015_558_Fig6_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 6 Ly-6G + cell depletion decreases the establishment and growth of liver metastases. (A) Schematic depicting the experimental protocol for depletion of Ly-6G + cells. (B) Paraffin-embedded liver sections were obtained following completion of the experimental metastasis assays and subjected to immunohistochemical staining with anti-Ly-6G antibodies. Statistically significant decreases in Ly-6G + cells that infiltrate the liver were observed when the neutrophil-depleted cohort was compared with the isotype control cohort ( P = 0.045). Representative images of Ly-6G-stained liver sections from each cohort. (C) Quantification of the tumor burden (tumor area/tissue area) within the cardiac liver lobe following splenic injection of 2776 liver-aggressive cells. Statistically significant decreases in liver-metastatic burden were observed when the isotype control cohort was compared with the neutrophil-depleted cohort ( P = 0.031). Representative images of hematoxylin and eosin (H&E)-stained liver sections exhibiting the liver-metastatic burden in each cohort. Dotted lines circumscribe breast cancer metastatic lesions within the liver. Scale bar represents 2 mm and applies to all panels. IP: intraperitoneal injection.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/14011282-25849774-CD11b-nihms662893f8.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Extended Data Fig. 4 Professional phagocytes are not present in regressing hair follicles a) Professional phagocytes do not enter regressing hair follicles. Single optical sections showing absence of myeloid populations inside the hair follicle 2.5 h after epithelial cell death (arrowhead), in LysM-Cre; tdTomato;K14-H2BGFP mice. b) Immunofluorescent staining of myeloid populations in skin during hair follicle regression. DAPI (blue), CD11b (red), and P-cadherin (green). Observations shown represent n = 4 mice. Scale bar, 25 mum</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/12031_2014_457_Fig4_HTML-20180918151446.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_2630_nihms-702371-f0003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/auto-10-972-g4-20180918151446.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/14011282-24849347-CD11b-zns9991456590001.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_5243_nihms562523f7.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/14011282-24583879-CD11b-zh50091465210005.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12584_3506fig1.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12582_zbc0311354400010.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/13011282-22700709-CD11b-i1552-5783-53-8-4575-f05.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/CD11b_22440612_IF_12_nihms361163f1_20180524.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/16011282-20488889-CD11b-awq107f2.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/nfig003_015-19-20190103084817.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_1457_1472-6750-9-1-2.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Immunohistochemistry (IHC)" title="CD11b Antibody (63-0112-82) in IHC"> <figcaption itemprop="caption">Figure 2 Survival of luciferase-expressing BMSC derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice . (A) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice. (B) Representative histological analysis of clonal BMSC-Luc grafts in the central nervous system of syngeneic immunocompetent mice. Week 1 post-implantation: Upper pictures, haematoxylin-eosin (HE) staining indicating localisation and general appearance of the implantation site. Lower left picture, Sca-1 staining indicating the BMSC origin of the observed cell graft. Lower right picture, CD11b staining indicating the presence of activated microglia surrounding the observed cell graft. Week 3 post-implantation: Upper pictures, HE staining indicating localisation and general appearance of the implantation site. Lower left picture, Sca-1 staining indicating the BMSC origin of the observed cell graft. Lower right picture, CD11b staining indicating the absence of activated microglia surrounding the observed cell graft. All slides were examined using a conventional bright field microscope and digital pictures were taken under magnification as indicated by the scale bars. (C) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29101_gr8.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Adrenergic signaling regulates CD11b expression in myeloid cells and controls migration into the liver after alphaGC treatment (A-E) C57BL/6J WT and Adra2ac -/- mice were treated with alphaGC (2mug/animal), and liver leukocytes were analyzed by flow cytometry after 3 h. Graphs represent the total number of liver leukocytes (CD45 +) (A), neutrophils (CD11b + Ly6G +) (B) and CD11b + F4/80 + cells (C), as well as the CD11b MFI in neutrophils (D) and CD11b + F4/80 + cells (E). (F-J) C57BL6/J WT animals were pre-treated with Yoh (5 mg/kg) or Phe (10 mg/kg) and then treated with alphaGC (2mug/animal). Liver leukocytes were analyzed by flow cytometry after 3 h, and graphs represent the total number of liver leukocytes (CD45 +) (F), neutrophils (CD11b + Ly6G +) (G) and CD11b + F4/80 + cells (H), as well as the CD11b MFI in neutrophils (I) and CD11b + F4/80 + cells (J). Data represent the mean +- SD from a single set of two independent experiments (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29102_41467_2023_42182_Fig4_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Terminal maturation of NK cells is associated with the downregulation of Eomes. a - c Newborn C57BL/6 mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. a On day 21 after infection, expression of Eomes and T-bet was determined by flow cytometry. b Eomes expression was analyzed on CD27 + CD11b - , CD27 - CD11b + , KLRG1 + and KLRG1 - NK cells from MCMV and mock-infected mice, representative histograms are shown. c Quantification of Eomes expressing CD27 + CD11b - , CD27 - CD11b + , KLRG1 + and KLRG1 - NK cells ( n = 9 mice per group, Mann-Whitney two-tailed test). Mean values +- SD are shown. d Newborn BALB/c and 129/SvJ mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. On day 21 after infection, mice were euthanized and the frequency of terminally differentiated, KLRG1 and Eomes expressing splenic NK cells was determined ( n = 4 Mock BALB/C, n = 5 MCMV BALB/C, n = 6 Mock 129/SvJ, n = 3 MCMV 129/SvJ, Mann-Whitney two-tailed test). Mean values +- SD are shown. e Newborn C57BL/6 mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. On days 60 and 90 after infection, mice were euthanized and the expression of KLRG1 and Eomes on NK cells was analyzed ( n = 3 Mock 60 d.p.i., n = 4 MCMV 60 d.p.i., n = 4 Mock 90 d.p.i., n = 6 MCMV 90 d.p.i., Mann-Whitney two-tailed test). Mean values +- SD are shown. f - h Adult C56BL/6 mice were infected with 2 x 10 5</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29102_41467_2023_42182_Fig6_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Conventional NK cells downregulate Eomes upon perinatal MCMV infection. Newborn mice were intraperitoneally infected with 200 PFU of MCMV or mock-infected 24-36 h after birth. a, b On day 21 post-infection of C56BL/6 mice, spleens and livers were harvested. a Expression of CD49b and CD49a was analyzed on NK1.1 + cells, N = 2. b Expression of CD27, CD11b and KLRG1 by ILC1s (CD49b - CD49a + ) was determined by flow cytometry in the liver, N = 2. c On day 21 post-infection of Ncr1 iCre Eomes f/f (KO) or Eomes f/f mice (WT), CD27, CD11b and KLRG1 expression by splenic NK1.1 + cells were determined by flow cytometry. Numbers of terminally differentiated and KLRG1 expressing NK1.1 + cells are shown ( n = 8 WT, n = 6 KO, Mann-Whitney two-tailed test). Mean values +- SD are shown. d On day 21 post-infection of C56BL/6 mice, spleens and bone marrow were harvested. Representative histograms of Eomes expression by NK cells is shown ( N = 3). Statistically significant differences are indicated ( P values). Source data are provided as a Source Data file.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29100_IJN-18-5495-g0006.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Intranasal delivery of EVs in the hydrogel inhibited inflammation. ( A ) Representative flow cytometry plots showing Ly6C high monocyte and Ly6C low monocytes/macrophages (CD45 + CD11b + ) in peripheral blood. and quantification of monocytes/macrophages (Ly6C high or low ) in peripheral blood. (n=5) ( B and C ). ( D ) Representative flow cytometry plots showing neutrophils (CD45 + CD11b + Ly6G + ) in peripheral blood and quantification of neutrophils in peripheral blood. n=5. ( E ). ( F ) Representative images of ventricles staining CD68 (green) showing the infiltration status of macrophages and quantification of CD68 + Cells. (n=5) ( G ). ELISA analysis of inflammatory factors IL-1 ( H ), IL-6 ( I ) and TNF-alpha ( J ) in peripheral blood. (n=5). (*P < 0.05, **P < 0.01, ***P < 0.001). Abbreviation : ns, not significant.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29099_fimmu-14-1230681-g003.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Figure 3 P. anaerobius promoted oxaliplatin resistance by recruiting MDSCs into the CRC microenvironment. (A) Viability of MC-38 cells co-cultured with P. anaerobius or in conditioned medium supplemented with PA supernatant (CCK-8 assay, p > 0.05, PA indicated by red arrow). (B) MDSCs (Gr-1 + CD11B + ) from bone marrow of CRC mice detected by multicolor flow cytometry. (C, D) MDSCs infiltrated into the tumor microenvironment detected by fluorescein isothiocyanate (FITC) with anti-mouse CD11b antibody (green), allophycocyanin (APC), anti-mouse Gr-1 antibody (red), and DAPI (blue). (E) Morphological feature of MDSCs collected by FCM (Giemsa staining, purple-blue leaf-shaped or round-type nucleus and almost colorless cytoplasm). (F) Chemotaxis ability of MDSCs treated with PA -CM, MC-38-CM, and PA +MC-38-CM (co-culture medium of PA and MC-38), respectively. (A-F) Data were presented as mean +- SD, p- values were determined by one-way analysis of variance. Three independent experiments were performed with consistent results.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g005.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Ablation of Cxcr2 in S100a8-expressing cells exacerbates SPEM development. (A) Breeding scheme of S100a8 Cre mice with Cxcr2 flox/flox mice to generate S100a8 Cre Cxcr2 flox/flox mice. (B) RT-qPCR analyses of Cxcr2 expression in FACS-isolated gastric CD11b + Ly6G + cells from 6-month H felis -infected S100a8 Cre Cxcr2 f/f versus Cxcr2 f/f stomachs. (C) Relative stomach weight in milligrams, normalized to total stomach weight in grams, in S100a8 Cre Cxcr2 flox/flox mice versus Cxcr2 flox/flox controls after 6mo H felis infection. (D) Percentage area of SPEM in the gastric mucosae of 6mo H felis -infected S100a8 Cre Cxcr2 flox/flox versus Cxcr2 flox/flox controls. SPEM quantification was performed using 4 longitudinal gastric strips, from the fundus and corpus, two of which were from the lesser and two from the greater curvatures of the stomach, and then by quantifying the percentage of 10x focal planes containing immunofluorescent co-localization of GSII and intrinsic factor, or expansion of GSII and total loss of intrinsic factor-expressing cells. (E) qPCR quantification of H felis flagellar filament B DNA, relative to genomic HPRT DNA, in 6mo H felis -infected S100a8 Cre Cxcr2 flox/flox versus Cxcr2 flox/flox controls. (F) Representative immunofluorescent staining of H + /K + -ATPase (red), GSII (green), and DAPI (blue) in the upper panels, in addition to intrinsic factor (IF, white) in the lower inset panels, in 6mo H felis -infected S100a8 Cre Cxcr2 flox/flox versus Cxcr2</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g001.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Gastric G-MDSCs are characterized by a specific transcriptional signature and can be identified by calprotectin (S100a8 and/or S100a9) expression. (A) Representative FACS plots of gastric MDSC versus non-MDSC myeloid cell populations using CD11b and Ly6G cell-surface protein markers, of dissociated gastric cells from uninfected versus 6mo H felis -infected WT mouse stomachs. Red font ""1"" annotates gastric non-myeloid and Ly6G-negative cells. Green font ""2"" annotates CD11b + Ly6G - non-G-MDSC myeloid cells. Black font ""3"" annotates gastric CD11b + Ly6G + G-MDSC myeloid cells. Blue font ""4"" annotates gastric CD11b - Ly6G + non-myeloid cells. (B) Microarray heatmap showing the highest expressed genes that are specifically enriched in gastric CD11b + Ly6G - non-G-MDSC myeloid cells (annotated as green font ""2""), versus those specifically enriched in gastric CD11b + Ly6G + G-MDSC myeloid cells (annotated as black font ""3""), and those specifically enriched in non-myeloid gastric CD11b - Ly6G + control cells (annotated as blue font ""4""). The gene names for the calprotectin subunits S100a8 and S100a9, Il1b, Slfn4, and Cxcr2 are listed (S100a8 and Cxcr2 are annotated in red font because they will later be used in the study for Cre/Flox generation). (C) Representative image of the immunohistochemical staining of calprotectin (brown) in 6mo H felis -infected mouse gastric mucosa relative to uninfected.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g006.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Cxcr2-deficient S100a8-expressing cells exhibit an altered expression of several genes associated with lipid metabolism, and an increase in the production of the lipid peroxidation product, 4-hydroxynonenal (4-HNE), within gastric epithelial lesions, following 6mo H felis infection. (A) Representative FACS plots of CD11b + Ly6G + G-MDSCs from H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/flox stomachs, which were used for sorting gastric G-MDSCs for microarray analyses. (B) Microarray heatmap of the MDSC-specific genes that exhibited altered expression patterns in G-MDSCs in response to Cre/Flox-mediated Cxcr2 ablation. Fabp5 is directly involved in lipid peroxidation and is annotated by a red arrow. (C) Immunohistochemical staining of 4-HNE staining (brown), within gastric epithelial lesions, in 6mo H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/flox stomachs. (D) Quantitative morphometry of 4-HNE staining. (E) Representative immunohistochemical staining of calprotectin (brown) in 6mo H felis -infected S100a8 Cre/+ Cxcr2 flox/flox versus Cxcr2 flox/flox stomachs. **P < 0.01.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29098_fimmu-14-1147695-g002.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Figure 2 S100a8 and S100a9 are enriched in gastric G-MDSCs but not in acutely recruited gut neutrophils. (A) Representative FACS plots of gastric G-MDSCs versus cecal neutrophils in 6-month H felis -infected stomachs versus 2-day C difficile infected ceca respectively. (B) Microarray heatmap showing the highest expressed genes that are specifically enriched in gastric CD11b + Ly6G + G-MDSCs relative to CD11b + Ly6G + cecal neutrophils in 6-month H felis -infected stomachs versus 2-day C difficile infected ceca respectively. The black arrows annotate the genes that are highly expressed in G-MDSCs of 6 month H felis -infected stomachs but not in neutrophils of 2-day C difficile infected ceca.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29097_gr3.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Effects of ACSL4 deficiency on PQ-induced lung inflammation. (A) mRNA expression levels of inflammatory cytokines (IL-6 and IL-1beta), COX-2, GPx4, and chemokines (CXCL1, CXCL2, CXCL5, and CXCR2) in lung tissues of untreated or PQ-administered WT (blue columns) and ACSL4-KO mice (red columns); n = 4/group. * p < 0.05 and ** p < 0.01, untreated WT vs PQ-administered WT mice; # p < 0.05 and ## p < 0.01, PQ-administered WT vs ACSL4 KO mice. (B) Representative Gr-1 staining images of lung tissue of untreated or PQ-administered WT and ACSL4-KO mice. The scale bars represent 100 mum. (C) Flow cytometry analysis of CD11b + Ly6G/6C + neutrophils (upper) and CD11 + F4/80+ macrophages (lower) in the BAL fluid cells of untreated or PQ-administered WT and ACSL4-KO mice; cell samples were pooled from 5 mice per group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Fig. 3</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29079_urn-x-wiley-0260437X-media-jat4496-jat4496-fig-0002.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">2 FIGURE Flow cytometric analysis of peritoneal macrophages using CD11b-PerCPCy5.5, CD86-PE, and CD206-FITC antibodies after single or dual blocking of toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1) and posttreatment with recombinant interleukin 10 (IL-10). Dot plot images of M1/M2 macrophage population analysis. Graphical representation of the CD86 + M1 population and graphical representation of the CD206 + M2 population in 11 different groups were shown. Data were represented as mean +- SD from three independent experiments. ""*"" indicates a significant difference in comparison with control in the non-IL-10-treated groups, ""#"" indicates a significant difference in comparison with lipopolysaccharide (LPS), ""*"" indicates a significant difference with respect to LPS + DEX, ""^"" indicates a significant difference with respect to TLR4Ab + LPS, ""EUR"" indicates significant difference with respect to TNFR1Ab + LPS, ""epsilon"" indicates significant difference with respect to control in the IL-10-treated group, ""delta"" indicates a significant difference in comparison with LPS + IL-10, ""$"" indicates significant difference with respect to LPS + DEX + IL-10, ""alpha"" indicates significant difference with respect to TLR4Ab + LPS + IL-10, and ""U"" indicates significant difference with respect to TNFR1Ab + LPS + IL-10. All the differences were statistically significant at p < 0.05 level.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24743_fphar-13-1095757-g002.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">FIGURE 2 Alteration of cellular composition in the diseased status of mice and humans. (A,B) Fractions of major cells types in aneurysmal aorta vs. the corresponding normal aorta of humans (A) and mice (B) . (C) Immunofluorescence analysis for SMMHC (red), alphaSMA (green), and overlays with DAPI-labeled nuclei (blue) showing smooth muscle cells in the human vascular media (scale bars: 100 mum). Lu means the lumen side of the vascular wall, and A means the adventitia side of the vascular wall. (D,E) Immunofluorescence analysis for vimentin (white) (D) and CD45 (purple) (E) and overlays with DAPI-labeled nuclei (blue) showing fibroblasts and immune cells in the human aorta (scale bars: 100 mum). (F) Immunostaining for calponin (red), alphaSMA (green), and overlays with DAPI-labeled nuclei (blue) showing smooth muscle cells in the mouse aortic media (scale bars: 100 mum). (G) Immunostaining for vimentin (white) and DAPI overlay in the thoracic and abdominal aorta form mice subjected to 4 weeks of Ang II induction (scale bars: 500 mum). (H) Immunostaining for CD45 (red), CD68 (green), and overlays with DAPI-labeled nuclei (blue) showing macrophages in the mouse aorta (scale bars: 100 mum). (I) Flow cytometry of the proportion of macrophages (F4/80 + CD11B + ), monocytes (LY6C + CD11B + ), and T cells (CD3 + ) in CD45 + immune cells among three groups (n = 3-4, per group).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24741_12276_2022_851_Fig3_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Deletion of Cxxc5 ameliorates NASH progression. a - m Cxxc5 +/+ and Cxxc5 -/- mice were fed a HFD for 12 weeks and injected with CCl 4 twice a week for the final 4 weeks to induce NASH ( n = 7 per group). a Representative IHC images of F4/80- and Cd11b-stained liver tissues (left panel). Quantification of hCLS formation and F4/80- and Cd11b-positive areas (right panel). Scale bars, 100 um. b Flow cytometry analyses of the expression of F4/80 and Cd11b (left panel). Percentage of F4/80 + Cd11b + cells ( n = 3) (right panel). c Inflammation score. d Relative mRNA expression levels of genes involved in pro-inflammation ( n = 3). e The NAFLD score. f Representative images of DHE-stained cells (left panel). Quantification of ROS formation by measuring DHE-positive cells in liver tissues (right panel). Scale bar, 100 um. g TUNEL staining of the liver (top panel). Quantification of TUNEL-positive cells (bottom panel). Scale bar, 100 um. h Relative mRNA expression of genes involved in cell death pathways (n = 3). i Representative IHC images for alpha-Sma, collagen I, desmin, and Sirius red staining of liver tissues. Scale bars, 100 um. j Relative mRNA expression of genes involved in fibrogenesis ( n = 3). k The fibrosis score. l , m Plasma concentrations of FFAs ( l ), AST and ALT ( m ). Data represent the mean +- SD. * P < 0.05; ** P < 0.01; *** P < 0.001 determined by Student's t test.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24737_urn-x-wiley-08941491-media-glia24255-glia24255-fig-0001.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">1 FIGURE Flow cytometry analysis of the CD11b+ cells. Cells isolated from non-perfused [(a)-(e)] and perfused [(f)-(j)] brains did not show significant differences. [(a), (f)] Histograms showing two CD45-expressing cell populations: CD45 low (microglia) and CD 45 high (possible macrophages). [(b), (g)] Microglia, identified as CD11b+ and CD45 low (Q3), comprised the highest proportion of cells (91.7%-94.5%), whereas a low percentage of potential macrophages (CD11b+/CD45 high, Q2) were observed. [(c), (h)] Forward scatter (FSC) analysis showing a slight smaller size of the CD45 low cells (red) respect to CD45 high (blue) cells. [(d), (i)] CD11b analysis showed a slight lower expression within CD45 low cells (red) respect to CD45 high (blue) cells. [(e), (j)] Forward scatter (FSC) and side scatter (SSC) characteristics of CD11b+/CD45 low (red) and CD11b+/CD45 high (blue) are shown</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20559_41467_2022_29730_Fig4_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Fig. 4 CAF-1 depletion induces myeloid differentiation in vivo and a mixed-lineage transcriptional signature in HSPCs. a Schematic of polyI:C (polyinosinic:polycytidylic acid) inducible Mx1-Cre/LoxP system in mice. Flow cytometric analysis of BM MNCs is performed 72 hrs post-induction of mice bearing homozygous Chaf1b floxed alleles and Mx1-Cre ( Chaf1b fl /fl ; Mx1-Cre) and littermate controls ( Chaf1b +/+ ; Mx1-Cre). b Flow cytometric analysis of myeloid differentiation markers (CD11b and Gr1) showing overlaid density plots and histograms of wild-type (Chaf1b +/+ ) and Chaf1b-deleted (Chaf1b Delta/ Delta ) BM MNCs. c Quantification of gated populations in b showing mean and standard deviation of CD11b and Gr1 double-positive and double negative % populations from live BM MNCs isolated from n = 2 wild-type and n = 3 knockout littermates. d Schematic of Chaf1b depletion in primary HSPCs. HSPCs isolated from homozygous floxed mice ( Chaf1b fl /fl ) are transduced with retroviral vectors, either empty control (MIGR1-CTRL) or overexpressing Cre recombinase (MIGR1-Cre) and analyzed by RNA-seq 48 h following expansion in myeloid media conditions. e RNA-seq analysis showing percentages and numbers of upregulated or downregulated genes in CAF-1 OFF iGMPs, HOXA9 OFF iGMPs, and Chaf1b Delta/ Delta HSPCs. DEGs are filtered based on fold change >= 1.5 and FDR < 0.05. f Venn diagrams of unique and common DEGs between CAF-1 OFF iGMPs, HOXA9 OFF iGMPs, and Chaf1b Delta/ Delta HSPCs shown i</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20557_gr5.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">The GOG stimulated BMSCs promoted the osteoclastogenesis of HSCs and the inhibited PERK pathway blocked the promotive effects of GOG on osteoblastic/osteoclastic differentiations. (a) The schematic diagram showed the designed induction procedure and scheduled detections at different timepoints. The HSCs were co-cultured with BMSCs in the transwell system for evaluation on the effects of GOG on osteoblastic and osteoclastic differentiations. The HSCs were cultured in the low chamber with 4 days of M-CSF (50 ng/mL) stimulus for inducing BMMs differentiation of HSCs. After 4 days M-CSF induction, the Flow cytometry analysis was conducted to evaluate the proportion of BMMs in total HSCs and the ALP staining was performed to evaluate the osteoblastic differentiation of BMSCs. The osteoclast differentiation medium containing 50 ng/mL M-CSF and 100 ng/mL RANKL was replaced at day 5 in the lower chamber for further analysis on osteoclastic differentiation of BMMs. In the subsequent induction of BMMs, the M1/M2 polarization was detected by M1/M2 specific markers (iNOS and arginase) immunofluorescent staining after 2 days of M-CSF + RANKL induction, and the calcium oscillation of BMMs was also detected after 2 days of M-CSF + RANKL induction. After 6 days of M-CSF + RANKL induction, the mature osteoclast differentiation from BMMs was verified by TRAP staining and the osteoblastic differentiation of BMSCs was evaluated by ARS staining. (b) The FACS analysis on the BMMs differentiation o</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24740_41467_2022_29471_Fig9_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Pharmacological inhibitor of MLCK MW01-022AZ suppressed tumor inflammation and growth. a Schematic of LLC lung tumor implantation in C57Bl6 mice and MW01-022AZ treatment. b-d Effect of MW01-022AZ on LLC b gross tumor morphology ( n = tumors from 6 mice) c tumor volume ( n = 9 mice and d tumor weight at endpoint (21 days) ( n = 9 mice). *p < 0.0001 for vehicle vs MW01-022AZ treatment, determined by t -test. e FACS profiles and f quantification of Gr1-CD11b+ ( p = 0.1146) Gr1loCD11b+ (* p = 0.0402), Gr1hiCD11b+ (* p = 0.0146) myeloid cells in representative MW01-022AZ vs vehicle-treated tumors at endpoint (21 days) ( n = 3 mice), determined by t -test. For Facs gating scheme, see Supplementary Fig. 7b . g Quantification and h representative histographs of F4/80+ macrophages ( n = sections from 6 mice) *p < 0.0001 for vehicle or MW01-022AZ by t -test, CD31+ blood vessels ( n = sections from 7 mice) *p < 0.0001 for vehicle or MW01-022AZ by t -test, CD8+ T cells ( n = sections from 9 mice) *p = 0.0244 for vehicle vs MW01-022AZ by Mann-Whitney test in tissues from mice treated with vehicle or MW01-022AZ . i Model of integrin activation demonstrating that cytokine stimulation activates a pathway whereby PI3Kgamma induces MLCK210 and myosin activation and colocalization with Rap1, resulting in Rap1-mediated integrin alpha4 conformation changes and interaction of the integrin with the actin cytoskeleton. Data are presented as mean values +- SEM. Statistical significance determined by</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_24740_41467_2022_29471_Fig7_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Deletion of bone marrow cell MLCK210 inhibits tumor growth. a Schematic of bone marrow transplantation and tumor implantation. b Genomic PCR to detect Mlck 210 gene in peripheral blood cells from BMT transplanted mice ( n = 2 mice). c - e Tumor volumes ( c ), weights at endpoint (15 days) (*p < 0.0001 WT vs Mlck210 -/- BM) ( d ) and images of gross tumor morphology ( e ) from LLC tumors in WT mice transplanted with bone marrow from WT ( n = 10 mice) or Mlck210 -/- mice ( n = 10 mice). f Representative FACS profiles and g quantification of Gr1-CD11b+ ( p = 0.9724) Gr1loCD11b+ (*p = 0.0280), Gr1hiCD11b+ ( p = 0.8641) myeloid cells in representative WT vs Mlck210 -/- BM transplanted tumors harvested at endpoint (15 days) ( n = 3 mice). For Facs gating scheme, see Supplementary Fig. 7b . h Representative histographs and i quantification of F4/80+ macrophages/field ( n = sections from 5 WT mice and 5 Mlck210 -/- ) *p = 0.0009, CD31+ blood vessels/field ( n = sections from 5 WT and 3 Mlck210 -/- mice) *p = 0.001, CD8+ T cells/field ( n = sections from 5 WT and 4 Mlck210 -/- mice) *p = 0.019 in tumor sections and 100X H&E-stained tumor sections to detect percent necrosis/field ( n = sections from 5 mice) *p = 0.0004 from mice transplanted with WT vs Mlck210 -/- bone marrow. Data are presented as mean values +- SEM. Significance testing was performed by Student's t -test. Experiments were performed twice. Source data are provided with this article as a Source Data file.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20556_pntd.0010224.g002.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">10.1371/journal.pntd.0010224.g002 Fig 2 During LdCen -/- ID infection, neutrophil derived increased CCL3 recruits significantly higher number of dendritic cells attributed with proinflammatory phenotype in the ear dLN compared to LdWT infection. (A) Parasitized neutrophils (Cd11b + Ly6G + Ly6c int RFP/mCherry + ) were flow sorted from the ear dLN 5d post infection and mRNA expression levels of CCL3 from sorted neutrophils in the ear dLN were estimated by qPCR as described in Materials and Methods and expressed as fold change from uninfected naive mice. The experiment was repeated three times with pooled digests from five to six ear dLNs per experiment. The data represent the mean values +- SD of results from three independent experiments that all yielded similar results (n = 5). ** p < 0.005 between the groups. (B) The gating strategy and individual flow plots for DCs (CD11b + Ly6G - Ly6C - CD11c + MHCII hi ) recruitment in ear dLNs 5d post infection is shown under CCL3 non-depleted or depleted condition. (C ) Total number of Cd11b + cells recruited in ear dLN 5 days post infection in mice depleted or not of CCL3 has been shown. (D) Changes in the total number of DCs per ear dLN. Values shown are the mean numbers of cells per ear dLN +- SD of results. 6-8 ear dLN, pooled data from 3 independent experiments that all yielded similar results (n = 6). *p< 0.05; ** p < 0.005, *** p < 0.0005 between the groups. ( E) Parasitized DCs (CD11b + CD11c + MHCII hi RFP/mCherry + ) were flo</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19070_41467_2021_27683_Fig9_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Fig. 9 OPN induces lysosomal-dependent lipid metabolism in BMDMs. a Representative western blots (left) and quantification (right, n = 3 biologically independent samples) of OPN receptor alphav and beta3 expression in BMDMs and metabolically activated macrophages (MMes) after culture for 4 days in vitro. b Schematic diagram illustrating the co-culture system as indicated below ( c ). c , d Representative images ( c ) and quantification ( n = 5 biologically independent samples) ( d ) of crystal violet staining of BMDMs (ctrl) and MMes treated with or without rOPN for 24 h in a Transwell system. Scale bar: 50 mum. e Immunofluorescence images of buoyant BMDMs (F4/80) containing lipid vesicles (Perilipin 1) isolated from bone marrow supernatant at week 12. Scale bar: 15 mum. f FACS analysis of FC + (F4/80+ and CD11b+ , top) and Perilipin1+ (bottom) BMDMs from NFD- (left) and HFD-fed (right) mice. P9, FC + and Perilipin1+ BMDM population from Q2. g Percentage of FC + and Perilipin1+ BMDMs ( n = 5 biologically independent samples). h Lactate concentration in bone marrow supernatant ( n = 5 biologically independent samples). i Schematic diagram illustrating the co-culture system as described below ( j ). j Immunofluorescence staining of the co-cultured BMDMs for 4 days in vitro; the eWAT from mice fed the HFD for 8 weeks was placed in the upper chamber; Neu Ab (2.0 mug/ml) was added into the lower chamber. Scale bar: 50 mum. The right-most image is an enlarged view of the field in t</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19070_41467_2021_27683_Fig5_HTML.jpg?time=20240507?time=20240507" alt="CD11b Antibody in Flow Cytometry (Flow)" title="CD11b Antibody (63-0112-82) in Flow"> <figcaption itemprop="caption">Fig. 5 OPN is primarily secreted by F4/80 + and CD11b+ macrophages in eWAT. a Representative images of immunofluorescence staining of OPN in eWAT from NFD- and HFD-fed mice at weeks 8, 12, and 16. Scale bar: 100 mum. b Quantification of the F4/80-positive or OPN-positive stained area percentage in eWAT from a ( n = 4 biologically independent samples). c Relative expression of Spp1 in the adipocyte fraction (AF) and stromal vascular fraction (SVF) from eWAT or iWAT of HFD-fed mice at week 12 ( n = 3 biologically independent samples). d ATMs gated by FACS for F4/80 and CD11b expression from eWAT at week 12 of NFD (left) or HFD feeding (right). Q2 represents the F4/80 + CD11b + (FC + ) ATM population, and Q3 represents the F4/80-CD11b- (FC-) ATM population. e Percentage of FC + ATMs from NFD- and HFD-fed mice at week 12 ( n = 4 biologically independent samples). f Relative expression of Spp1 in FC+ and FC- ATMs from HFD-fed mice at week 12 ( n = 3 biologically independent samples). Images are representative of 3 independent experiments. All data are presented as mean +- SD. Two-way ANOVA with Sidak's post-hoc test ( b ) and two-tailed Welch's t -test ( c , e , f ) were used. Source data are provided as a Source Data file.</figcaption> </figure> </div> </div> </noscript> <div id="tf-related-products-container-new"></div> <div class="product-details"> <div class="container"> <div class="row"> <div class="large-span-12 medium-span-12 small-span-12"> <h2 class="pdp-sub-heading product_details_heading">Product Details</h2></div> <div class="hidden-sku"> <h2>63-0112-82</h2> </div> <div class="product-detail-section tested-app-section new-tbl-design product-detail-section table-spec"> <div class="product-container-head"> <div class="product-item"> Applications </div> <div class="product-item dilution-lbl-head "> Tested Dilution </div> <div class="product-item published-applications"> Publications </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (IHC)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHC-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 43 publications</span> <span class="mobile-view">43 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (PFA fixed) (IHC (PFA))</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHCPFA-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 5 publications</span> <span class="mobile-view">5 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (Frozen) (IHC (F))</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHCF-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 3 publications</span> <span class="mobile-view">3 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunocytochemistry (ICC/IF)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ICCIF-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 17 publications</span> <span class="mobile-view">17 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Flow Cytometry (Flow)</h2></div> <div class="product-item "> <span class="dilution-lbl">0.125 µg/test</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Flow-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 243 publications</span> <span class="mobile-view">243 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunoprecipitation (IP)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IP-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Neutralization (Neu)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Neu-content"]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 2 publications</span> <span class="mobile-view">2 publications</span></a> </div> </div> </div> <div class="product-detail-section tested-app-section new-tbl-design product-specific-section table-spec"> <div class="product-container-head"> <div class="product-item">Product Specifications</div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Species Reactivity</h2> </div> <div class="product-item left-flex-cls"> <span title="Mus musculus">Mouse</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Published species</h2> </div> <div class="product-item left-flex-cls"> <span>Fish,</span> <span>Human,</span> <span>Mouse</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2> Host/Isotype</h2> </div> <div class="product-item left-flex-cls"> <span title="Rattus">Rat / IgG2b, kappa</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Recommended Isotype Control</h2> </div> <div class="product-item left-flex-cls"> <a href="//www.thermofisher.com/antibody/product/63-4031-82"> Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™</a> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Class</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Monoclonal </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Type</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Antibody </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Clone</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> M1/70 </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Conjugate</h2> </div> <div class="product-item left-flex-cls"> <a ng-if="$ctrl.checkConjugate('Super Bright™ 600 ') && '/us/en/home/life-science/cell-analysis/fluorophores/super-bright-600.html?cid=bid_pca_aup_r01_co_cp1603_pjt0000_abs00000_0wm_tfs_il_crs_cs_s00_dye'.trim() != ''" href="/us/en/home/life-science/cell-analysis/fluorophores/super-bright-600.html?cid=bid_pca_aup_r01_co_cp1603_pjt0000_abs00000_0wm_tfs_il_crs_cs_s00_dye" ng-attr-target="{{$ctrl.getRequestCustomLinkTarget()}}" class="conjugate-value">Super Bright™ 600 </a> <span ng-if="($ctrl.checkConjugate('Super Bright™ 600 ') && '/us/en/home/life-science/cell-analysis/fluorophores/super-bright-600.html?cid=bid_pca_aup_r01_co_cp1603_pjt0000_abs00000_0wm_tfs_il_crs_cs_s00_dye'.trim() == '')" class="conjugate-value">Super Bright™ 600 </span> <span ng-if="!$ctrl.checkConjugate('Super Bright™ 600 ')" class="conjugate-value">Super Bright™ 600 </span> <div class="dropdown conjugate-dropdown"> <button class="btn-link conjugate-btn" type="button" data-toggle="dropdown"> View additional formats <span class="caret"></span> </button> <ul class="dropdown-menu dropdown-menu-fixed"> <li><a class="conjugate-color-padding" href="/antibody/product/14-0112-82"> Unconjugated <span class="conjugate-indicator" style="background-color:"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/53-0112-82"> Alexa Fluor 488 <span class="conjugate-indicator" style="background-color:#36FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/58-0112-82"> Alexa Fluor 532 <span class="conjugate-indicator" style="background-color:#B3FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/606-0112-82"> Alexa Fluor 660 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/56-0112-82"> Alexa Fluor 700 <span class="conjugate-indicator" style="background-color:#8D0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/17-0112-82"> APC <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/47-0112-82"> APC-eFluor 780 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/13-0112-82"> Biotin <span class="conjugate-indicator" style="background-color:"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/363-0112-82"> Brilliant UV 395 <span class="conjugate-indicator" style="background-color:#8000A1"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/364-0112-82"> Brilliant UV 496 <span class="conjugate-indicator" style="background-color:#00FFFF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/365-0112-82"> Brilliant UV 563 <span class="conjugate-indicator" style="background-color:#C3FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/366-0112-82"> Brilliant UV 615 <span class="conjugate-indicator" style="background-color:#FF8900"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/376-0112-82"> Brilliant UV 661 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/367-0112-82"> Brilliant UV 737 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/368-0112-82"> Brilliant UV 805 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/404-0112-82"> Brilliant Violet 421 <span class="conjugate-indicator" style="background-color:#5400FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/414-0112-82"> Brilliant Violet 480 <span class="conjugate-indicator" style="background-color:#00C0FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/406-0112-82"> Brilliant Violet 605 <span class="conjugate-indicator" style="background-color:#FFAD00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/416-0112-82"> Brilliant Violet 650 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/407-0112-82"> Brilliant Violet 711 <span class="conjugate-indicator" style="background-color:#820000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/417-0112-82"> Brilliant Violet 786 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/48-0112-82"> eFluor 450 <span class="conjugate-indicator" style="background-color:#0028FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/69-0112-82"> eFluor 506 <span class="conjugate-indicator" style="background-color:#00FF54"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/50-0112-82"> eFluor 660 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/11-0112-82"> FITC <span class="conjugate-indicator" style="background-color:#1FFF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/16-0112-82"> Functional Grade <span class="conjugate-indicator" style="background-color:"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02B01-A"> NovaFluor Blue 510 <span class="conjugate-indicator" style="background-color:#00FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02B04-A"> NovaFluor Blue 585 <span class="conjugate-indicator" style="background-color:#FFEF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02B05-A"> NovaFluor Blue 610-30S <span class="conjugate-indicator" style="background-color:#FF8900"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02B07-A"> NovaFluor Blue 660-40S <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02R03"> NovaFluor Red 700 <span class="conjugate-indicator" style="background-color:#8D0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02Y01-A"> NovaFluor Yellow 570 <span class="conjugate-indicator" style="background-color:#E1FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02Y02-A"> NovaFluor Yellow 590 <span class="conjugate-indicator" style="background-color:#FFDF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/M015T02Y04-A"> NovaFluor Yellow 660 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/12-0112-82"> PE <span class="conjugate-indicator" style="background-color:#F0FF00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/15-0112-82"> PE-Cyanine5 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/25-0112-82"> PE-Cyanine7 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/61-0112-82"> PE-eFluor 610 <span class="conjugate-indicator" style="background-color:#FF9B00"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/45-0112-82"> PerCP-Cyanine5.5 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/46-0112-82"> PerCP-eFluor 710 <span class="conjugate-indicator" style="background-color:#820000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/62-0112-82"> Super Bright 436 <span class="conjugate-indicator" style="background-color:#3D00FF"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/64-0112-82"> Super Bright 645 <span class="conjugate-indicator" style="background-color:#DB0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/67-0112-82"> Super Bright 702 <span class="conjugate-indicator" style="background-color:#8D0000"></span> </a></li> <li><a class="conjugate-color-padding" href="/antibody/product/78-0112-82"> Super Bright 780 <span class="conjugate-indicator" style="background-color:#808080"></span> </a></li> <li class="drop-down-link-ctnr"> <a class="conjugate-dropdown-link" href="/us/en/home/global/forms/antibody-conjugates-custom-form.html" ng-attr-target="$ctrl.getRequestCustomLinkTarget()">Request custom conjugation <svg class="svg-icon external-link-icon" ng-if="$ctrl.showExternalLinkIcon()"> <use href="#external-link"></use> </svg> </a> </li> </ul> </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Excitation/Emission Max</h2> </div> <div class="product-item left-flex-cls"> <span>414/601 nm</span> <a target="_blank" class="spectra-view" href="/order/fluorescence-spectraviewer?SID=srch-svtool&UID=sb600"> <span class="view-spectra"> View spectra</span> <span class="spectra-view-icon "><img src="/order/genome-database/assets/img/spectra.png" alt="spectra"/></span> </a> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Form</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Liquid </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Concentration</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.2 mg/mL </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Purification</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Affinity chromatography </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage buffer</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> PBS, pH 7.2, with BSA </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Contains</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.09% sodium azide </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 4° C, store in dark, DO NOT FREEZE! </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Shipping conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Ambient (domestic); Wet ice (international) </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"><h2>RRID <span id="rrid-tooltip" class="rrid-tooltip-icon" ng-include="'/order/genome-database/webapp/assets/core/img/svg/mono_tooltip.svg'" data-toggle="tooltip" ng-click="$ctrl.showRridTooltip()" close-tooltip="$ctrl.closeRridTooltip()" title="Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources in the biomedical literature to improve transparency of research methods."> </span></h2> </div> <div class="product-item left-flex-cls">AB_2637408</div> </div> </div> <div class="product-specific-details"> <div class="product-specific-details-tbl product-specific-add-border" > <div class="product-info-para"> <h4 class="product-info-para-heading"> Product Specific Information </h4> <p>Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaM-beta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.<br /><br />Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.<br /><br />Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br /><br />Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.<br /><br />When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href="https://www.thermofisher.com/order/catalog/product/SB-4401-42">Product # SB-4401</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.<br /><br />Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.<br /><br />Fixation: Samples can be stored in IC Fixation Buffer (<a href="https://www.thermofisher.com/order/catalog/product/00-8222-49">Product # 00-8222</a>) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href="https://www.thermofisher.com/order/catalog/product/00-5333-57">Product # 00-5333</a>) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.<br /><br />Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser<br /><br />Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.</p> </div> <div class="product-info-para"> <h4 class="product-info-para-heading"> Target Information </h4> <p class="product-specific-target-info">CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.</p> </div> <p class="product-details-regulatoryStmt">For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.</p> </div> <div class="product-detail-panel-builder"> <tf-promo-ad ng-if="$ctrl.showPromoAd" show-promo-ad="$ctrl.showPromoAd"></tf-promo-ad> <div class="product-detail-video"> <div tf-video-player-new video-id="6206990159001" player-width="368" player-height="210"></div> <div class="panel-builder"> <h4 class="product-info-para-heading"> How to use the Panel Builder </h4> <p>Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.</p> </div> </div> </div> </div> </div> </div> </div> <div id="rootPdpDocLoader"></div> <div id="tf-related-products-container" ng-show="emptyRelatedProductsWidget === false" > <related-products-container ng-if="showRelatedProductsWidget === true" base-url="'/api/magellan/related-products-ui/widget/v2/'" product-type="'antibody'" product-sub-type="'primary'" product-id="'63-0112-82'" country-code="'sg'" iso-language="'en'" > </related-products-container> </div> <div class="tf-pdp-divider" ng-if="emptyRelatedProductsWidget === false" ></div> <div class="references reference-section-3" id="references-component-id"> <div id="references-section-id"> <reference-section ng-if="loadReferences" default-display-card-limit="3" reference-count="314" sub-type="'Antibody'" application-data='{"citationApplications":[{"applicationAbbreviation":"IHC","citations":[{"title":"Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of \u003ci\u003ePseudomonas aeruginosa\u003c/i\u003e.","summary":"Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry","authors":"Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X","literatureId":"36514779","journal":"MedComm","volume":"3","publishedDate":"Dec 1, 2022, 5:00:00 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63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Nov 03 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36703732"> <div itemprop="name">Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level.</div> </a> <div itemprop="about">Frontiers in pharmacology</div> <div itemprop="author">Wu H,Xie C,Wang R,Cheng J,Xu Q,Zhao H</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Feb 02 00:00:00 EST 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35943203"> <div itemprop="name">Microglial angiotensin type 2 receptors mediate sex-specific expression of inflammatory cytokines independently of circulating estrogen.</div> </a> <div itemprop="about">Glia</div> <div itemprop="author">Garrido-Gil P,Pedrosa MA,Garcia-Garrote M,Pequeño-Valtierra A,Rodríguez-Castro J,García-Souto D,Rodríguez-Pérez AI,Labandeira-Garcia JL</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Dec 01 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36514779"> <div itemprop="name">Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of <i>Pseudomonas aeruginosa</i>.</div> </a> <div itemprop="about">MedComm</div> <div itemprop="author">Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</div> <div itemprodp="datePublished">Thu Dec 01 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35487911"> <div itemprop="name">Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Franklin R,Guo Y,He S,Chen M,Ji F,Zhou X,Frankhouser D,Do BT,Chiem C,Jang M,Blanco MA,Vander Heiden MG,Rockne RC,Ninova M,Sykes DB,Hochedlinger K,Lu R,Sadreyev RI,Murn J,Volk A,Cheloufi S</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Fri Apr 29 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35386350"> <div itemprop="name">Biocompatible reduced graphene oxide stimulated BMSCs induce acceleration of bone remodeling and orthodontic tooth movement through promotion on osteoclastogenesis and angiogenesis.</div> </a> <div itemprop="about">Bioactive materials</div> <div itemprop="author">Jiao D,Wang J,Yu W,Zhang K,Zhang N,Cao L,Jiang X,Bai Y</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Sep 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35795081"> <div itemprop="name">Ionizable Lipid Nanoparticle-Mediated Delivery of Plasmid DNA in Cardiomyocytes.</div> </a> <div itemprop="about">International journal of nanomedicine</div> <div itemprop="author">Scalzo S,Santos AK,Ferreira HAS,Costa PA,Prazeres PHDM,da Silva NJA,Guimarães LC,E Silva MM,Rodrigues Alves MTR,Viana CTR,Jesus ICG,Rodrigues AP,Birbrair A,Lobo AO,Frezard F,Mitchell MJ,Guatimosim S,Guimaraes PPG</div> <div itemprop="description">63-0112-82 was used in Flow cytometry/Cell sorting to highlight the potential of LNPs as an efficient delivery vector for pDNA to cardiomyocytes.</div> <div itemprodp="datePublished">Fri Jul 08 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35970323"> <div itemprop="name">Sex-Dependent Hepatoprotective Role of IL-22 Receptor Signaling in Non-Alcoholic Fatty Liver Disease-Related Fibrosis.</div> </a> <div itemprop="about">Cellular and molecular gastroenterology and hepatology</div> <div itemprop="author">Abdelnabi MN,Flores Molina M,Soucy G,Quoc-Huy Trinh V,Bédard N,Mazouz S,Jouvet N,Dion J,Tran S,Bilodeau M,Estall JL,Shoukry NH</div> <div itemprop="description">63-0112-82 was used in Flow cytometry/Cell sorting to suggest a sex-dependent hepatoprotective antiapoptotic effect of IL22-receptor signaling during NAFLD-related liver injury in females.</div> <div itemprodp="datePublished">Tue Nov 22 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35849045"> <div itemprop="name">A novel ZsGreen knock-in melanoma cell line reveals the function of CD11b in tumor phagocytosis.</div> </a> <div itemprop="about">Immunology and cell biology</div> <div itemprop="author">Zhang L,Feng X,Shen Y,Wang Y,Liu Z,Ma Y,Gu Y,Guo G,Duan L,Lu L,Liang Y,Lawrence T,Huang R</div> <div itemprop="description">63-0112-82 was used in Flow cytometry/Cell sorting to demonstrate that the ZsGreen-expressing YUMM1.7 melanoma model provides a valuable tool for the study of phagocytosis in vivo.</div> <div itemprodp="datePublished">Sat Oct 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35874469"> <div itemprop="name">Protocol to establish a stable <i>MLL-AF9</i>_AML mouse model.</div> </a> <div itemprop="about">STAR protocols</div> <div itemprop="author">Lu J,Zhao H,Yang L,Jiang X</div> <div itemprop="description">63-0112-82 was used in Flow cytometry/Cell sorting to describe a mouse MLL-AF9_AML model to investigate Acute myeloid leukemia.</div> <div itemprodp="datePublished">Fri Sep 16 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35269588"> <div itemprop="name">Epigenetic Studies in the Male APP/BIN1/COPS5 Triple-Transgenic Mouse Model of Alzheimer's Disease.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Martínez-Iglesias O,Naidoo V,Carrera I,Cacabelos R</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</div> <div itemprodp="datePublished">Wed Feb 23 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33388094"> <div itemprop="name">Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.</div> </a> <div itemprop="about">Cell host & microbe</div> <div itemprop="author">Guo Q,Zhao Y,Li J,Liu J,Yang X,Guo X,Kuang M,Xia H,Zhang Z,Cao L,Luo Y,Bao L,Wang X,Wei X,Deng W,Wang N,Chen L,Chen J,Zhu H,Gao R,Qin C,Wang X,You F</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Wed Feb 10 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33300142"> <div itemprop="name">Mice with a specific deficiency of Pfkfb3 in myeloid cells are protected from hypoxia-induced pulmonary hypertension.</div> </a> <div itemprop="about">British journal of pharmacology</div> <div itemprop="author">Wang L,Zhang X,Cao Y,Ma Q,Mao X,Xu J,Yang Q,Zhou Y,Lucas R,Fulton DJ,Su Y,Barman SA,Hong M,Liu Z,Huo Y</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon Mar 01 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33046512"> <div itemprop="name">Depletion of Adipocyte <i>Becn1</i> Leads to Lipodystrophy and Metabolic Dysregulation.</div> </a> <div itemprop="about">Diabetes</div> <div itemprop="author">Jin Y,Ji Y,Song Y,Choe SS,Jeon YG,Na H,Nam TW,Kim HJ,Nahmgoong H,Kim SM,Kim JW,Nam KT,Seong JK,Hwang D,Park CB,Lee IH,Kim JB,Lee HW</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</div> <div itemprodp="datePublished">Fri Jan 01 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33296111"> <div itemprop="name">The effects of postmortem delay on mouse and human microglia gene expression.</div> </a> <div itemprop="about">Glia</div> <div itemprop="author">Heng Y,Dubbelaar ML,Marie SKN,Boddeke EWGM,Eggen BJL</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Apr 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33068332"> <div itemprop="name">Intrinsic DNA damage repair deficiency results in progressive microglia loss and replacement.</div> </a> <div itemprop="about">Glia</div> <div itemprop="author">Zhang X,Heng Y,Kooistra SM,van Weering HRJ,Brummer ML,Gerrits E,Wesseling EM,Brouwer N,Nijboer TW,Dubbelaar ML,Boddeke EWGM,Eggen BJL</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon Mar 01 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34092215"> <div itemprop="name">CD36 deficiency ameliorates drug-induced acute liver injury in mice.</div> </a> <div itemprop="about">Molecular medicine (Cambridge, Mass.)</div> <div itemprop="author">Zhang C,Shi X,Su Z,Hu C,Mu X,Pan J,Li M,Teng F,Ling T,Zhao T,Xu C,Ji G,You Q</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 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63-0112-82) in Immunocytochemistry</div> <div itemprodp="datePublished">Fri Mar 25 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34071825"> <div itemprop="name">Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells.</div> </a> <div itemprop="about">Biology</div> <div itemprop="author">Hridi SU,Barbour M,Wilson C,Franssen AJ,Harte T,Bushell TJ,Jiang HR</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry (Frozen)</div> <div itemprodp="datePublished">Thu May 27 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33981156"> <div itemprop="name">Dual Activity of Ginsenoside Rb1 in Hypertrophic Cardiomyocytes and Activated Macrophages: Implications for the Therapeutic Intervention of Cardiac Hypertrophy.</div> </a> <div itemprop="about">Journal of inflammation research</div> <div itemprop="author">Wang S,Cui Y,Xiong M,Li M,Wang P,Cui J,Du X,Chen Y,Zhang T</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</div> <div itemprodp="datePublished">Sat Apr 23 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33810600"> <div itemprop="name"><i>Mycobacterium tuberculosis</i> Load in Host Cells and the Antibacterial Activity of Alveolar Macrophages Are Linked and Differentially Regulated in Various Lung Lesions of Patients with Pulmonary Tuberculosis.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Ufimtseva EG,Eremeeva NI,Umpeleva TV,Vakhrusheva DV,Skornyakov SN</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry</div> <div itemprodp="datePublished">Fri Mar 26 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34164439"> <div itemprop="name">Serp-1 Promotes Corneal Wound Healing by Facilitating Re-epithelialization and Inhibiting Fibrosis and Angiogenesis.</div> </a> <div itemprop="about">Frontiers in cardiovascular medicine</div> <div itemprop="author">Ju B,Guo O,Benissan-Messan DZ,Shawver MH,Chen P,Geng B,Wei S,Yaron JR,Lucas AR,Zhu H</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry (PFA fixed)</div> <div itemprodp="datePublished">Fri May 20 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34164439"> <div itemprop="name">Serp-1 Promotes Corneal Wound Healing by Facilitating Re-epithelialization and Inhibiting Fibrosis and Angiogenesis.</div> </a> <div itemprop="about">Frontiers in cardiovascular medicine</div> <div itemprop="author">Ju B,Guo O,Benissan-Messan DZ,Shawver MH,Chen P,Geng B,Wei S,Yaron JR,Lucas AR,Zhu H</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunohistochemistry (PFA fixed)</div> <div itemprodp="datePublished">Fri May 20 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34370755"> <div itemprop="name">Developmental chronodisruption alters placental signaling in mice.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Clarkson-Townsend DA,Bales KL,Hermetz KE,Burt AA,Pardue MT,Marsit CJ</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Immunocytochemistry</div> <div itemprodp="datePublished">Wed Nov 24 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34001904"> <div itemprop="name">Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Jin H,Liu K,Tang J,Huang X,Wang H,Zhang Q,Zhu H,Li Y,Pu W,Zhao H,He L,Li Y,Zhang S,Zhang Z,Zhao Y,Qin Y,Pflanz S,Kasmi KEI,Zhang W,Liu Z,Ginhoux F,Ji Y,He B,Wang L,Zhou B</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon May 17 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34001904"> <div itemprop="name">Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Jin H,Liu K,Tang J,Huang X,Wang H,Zhang Q,Zhu H,Li Y,Pu W,Zhao H,He L,Li Y,Zhang S,Zhang Z,Zhao Y,Qin Y,Pflanz S,Kasmi KEI,Zhang W,Liu Z,Ginhoux F,Ji Y,He B,Wang L,Zhou B</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon May 17 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33830083"> <div itemprop="name">Brd4 modulates diet-induced obesity via PPARγ-dependent Gdf3 expression in adipose tissue macrophages.</div> </a> <div itemprop="about">JCI insight</div> <div itemprop="author">Hu X,Dong X,Li G,Chen Y,Chen J,He X,Sun H,Kim DH,Kemper JK,Chen LF</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Thu Apr 08 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34831405"> <div itemprop="name">Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.</div> </a> <div itemprop="about">Cells</div> <div itemprop="author">Jhan MK,Chen CL,Shen TJ,Tseng PC,Wang YT,Satria RD,Yu CY,Lin CF</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon Nov 15 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34252585"> <div itemprop="name">Pancreatic Cancer Chemotherapy Is Potentiated by Induction of Tertiary Lymphoid Structures in Mice.</div> </a> <div itemprop="about">Cellular and molecular gastroenterology and hepatology</div> <div itemprop="author">Delvecchio FR,Fincham REA,Spear S,Clear A,Roy-Luzarraga M,Balkwill FR,Gribben JG,Bombardieri M,Hodivala-Dilke K,Capasso M,Kocher HM</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Mon Mar 28 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34289451"> <div itemprop="name">Myeloid DJ-1 deficiency protects acetaminophen-induced acute liver injury through decreasing inflammatory response.</div> </a> <div itemprop="about">Aging</div> <div itemprop="author">Wang B,Li J,Jiao J,Xu M,Luo Y,Wang F,Xia Q,Gao Y,Feng Y,Kong X,Sun X</div> <div itemprop="description">Published figure using CD11b monoclonal antibody (Product # 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itemprop="description">Published figure using CD11b monoclonal antibody (Product # 63-0112-82) in Flow Cytometry</div> <div itemprodp="datePublished">Fri Oct 24 00:00:00 EDT 2008</div> </div> </noscript> </div> <div class="border-bottom"></div> <div class="product-bioinformatics-details"> <div class="container"> <h2 class="pdp-sub-heading">Bioinformatics</h2> <div class="row"> <div class="large-span-6 medium-span-12 small-span-12"> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="proteinContent"> <strong>Protein Aliases:</strong> CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044 </p> <p class="view-buttons" id="ptnViewBtn" ng-class="$ctrl.bioContentLines('proteinContent', 'ptnViewBtn')"> <a id="proteinViewMoreBtn" ng-click="$ctrl.bioContentViewMore('proteinViewMoreBtn', 'proteinViewLessBtn', 'proteinContent')"> View more </a> <a id="proteinViewLessBtn" ng-click="$ctrl.bioContentViewLess('proteinViewLessBtn', 'proteinViewMoreBtn', 'proteinContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="geneContent"> <strong>Gene Aliases:</strong> Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1 </p> <p class="view-buttons" id="geneViewBtn" ng-class="$ctrl.bioContentLines('geneContent', 'geneViewBtn')"> <a id="geneViewMoreBtn" ng-click="$ctrl.bioContentViewMore('geneViewMoreBtn', 'geneViewLessBtn', 'geneContent')"> View more </a> <a id="geneViewLessBtn" ng-click="$ctrl.bioContentViewLess('geneViewLessBtn', 'geneViewMoreBtn', 'geneContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="entrezContent"> <strong>Entrez Gene ID:</strong> <a href="http://www.ncbi.nlm.nih.gov/gene?term=16409" target="_blank">(Mouse) 16409</a> </p> <p class="view-buttons" id="etzViewBtn" ng-class="$ctrl.bioContentLines('entrezContent', 'etzViewBtn')"> <a id="entrezViewMoreBtn" ng-click="$ctrl.bioContentViewMore('entrezViewMoreBtn', 'entrezViewLessBtn', 'entrezContent')"> View more </a> <a id="entrezViewLessBtn" ng-click="$ctrl.bioContentViewLess('entrezViewLessBtn', 'entrezViewMoreBtn', 'entrezContent')"> View less </a> </p> </div> </div> <div class="large-span-6 medium-span-12 small-span-12"> <div class="accordion" id="accordion-bioinformatics"> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseOne"> Function(s) </a> </div> <div id="collapseOne" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/opsonin binding"> opsonin binding </a> <a class="icon" href="/antibody/primary/panther/glycoprotein binding"> glycoprotein binding </a> <a class="icon" href="/antibody/primary/panther/heparin binding"> heparin binding </a> <a class="icon" href="/antibody/primary/panther/heparan sulfate proteoglycan binding"> heparan sulfate proteoglycan binding </a> <a class="icon" href="/antibody/primary/panther/metal ion binding"> metal ion binding </a> <a class="icon" href="/antibody/primary/panther/protein heterodimerization activity"> protein heterodimerization activity </a> <a class="icon" href="/antibody/primary/panther/integrin"> integrin </a> </div> </div> </div> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseTwo"> Process(es) </a> </div> <div id="collapseTwo" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/leukocyte migration involved in inflammatory response"> leukocyte migration involved in inflammatory response </a> <a class="icon" href="/antibody/primary/panther/cell adhesion"> cell adhesion </a> <a class="icon" href="/antibody/primary/panther/leukocyte cell-cell adhesion"> leukocyte cell-cell adhesion </a> <a class="icon" href="/antibody/primary/panther/integrin-mediated signaling pathway"> integrin-mediated signaling pathway </a> <a class="icon" href="/antibody/primary/panther/microglia development"> microglia development </a> <a class="icon" href="/antibody/primary/panther/single organismal cell-cell adhesion"> single organismal cell-cell adhesion </a> <a class="icon" href="/antibody/primary/panther/neutrophil chemotaxis"> neutrophil chemotaxis </a> <a class="icon" href="/antibody/primary/panther/cellular extravasation"> cellular extravasation </a> <a class="icon" href="/antibody/primary/panther/activated T cell proliferation"> activated T cell proliferation </a> </div> </div> </div> </div> </div> </div> </div> </div> <div class="recommended-products border-bottom hidden"> <!-- TODO: --> It has to be done as per old AB suggested Products section. </div> <script type="text/javascript" src="/order/genome-database/assets/core/js/magellanCore.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript"> $m.addPerformanceMark('abPDPScripts:loadBegin'); </script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/angular108-document-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/pdp-combined-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" 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Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br /><br />Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.<br /><br />When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href="https://www.thermofisher.com/order/catalog/product/SB-4401-42">Product # SB-4401</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.<br /><br />Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.<br /><br />Fixation: Samples can be stored in IC Fixation Buffer (<a href="https://www.thermofisher.com/order/catalog/product/00-8222-49">Product # 00-8222</a>) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href="https://www.thermofisher.com/order/catalog/product/00-5333-57">Product # 00-5333</a>) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. 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