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<!doctype html> <html lang="pt"> <head> <title>Modification of a Targeted Metabolomics Method Using Hydroph | 105032</title> <meta name="keywords" content="Chie Saito Ishii1*, Karen C Scott1, Carolina Abrahan2, Joy G. Cagmat2, Taylor A. Harmon3, Yann Queau4, Vincent Biourge4, Timothy J. Garrett5, Richard C Hill1, "/> <meta name="description" content="Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths becaus..105032"/> <meta name="citation_publisher" content="Longdom Publishing SL" /> <meta name="citation_journal_title" content="Jornal de Cromatografia e T茅cnicas de Separa莽茫o"> <meta name="citation_title" content="Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods"> <meta name="citation_author" content="Chie Saito Ishii1" /> <meta name="citation_author" content="Karen C Scott1" /> <meta name="citation_author" content="Carolina Abrahan2" /> <meta name="citation_author" content="Joy G. Cagmat2" /> <meta name="citation_author" content="Taylor A. Harmon3" /> <meta name="citation_author" content="Yann Queau4" /> <meta name="citation_author" content="Vincent Biourge4" /> <meta name="citation_author" content="Timothy J. Garrett5" /> <meta name="citation_author" content="Richard C Hill1" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="14"> <meta name="citation_issue" content="6"> <meta name="citation_abstract" content="Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries were within 100% ± 20% for adenine, guanine, xanthine, adenosine, inosine, guanosine, and adenosine monophosphate at added purine concentrations similar to those in a low and a high purine food chosen to assess quality control."> <meta name="citation_abstract_html_url" content="https://portuguese.longdom.org/abstract/modification-of-a-targeted-metabolomics-method-using-hydrophilic-interaction-liquid-chromatographyhigh-resolution-mass-s-105032.html"> <meta name="format-detection" content="telephone=no"> <meta http-equiv="Content-Language" content="pt"> <meta name="google-site-verification" content="NomPTP94YozsgvD3NEFpNqUfY88e0TU0L64zNzZTpd0" /> <meta itemprop="name" content="longdom" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="ROBOTS" content="INDEX,FOLLOW"> <meta name="googlebot" content="INDEX,FOLLOW"> <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> <link rel="canonical" 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Saito Ishii1*, Karen C Scott1, Carolina Abrahan2, Joy G. Cagmat2, Taylor A. Harmon3, Yann Queau4, Vincent Biourge4, Timothy J. Garrett5, Richard C Hill1 </p> <p>Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries were within 100% ± 20% for adenine, guanine, xanthine, adenosine, inosine, guanosine, and adenosine monophosphate at added purine concentrations similar to those in a low and a high purine food chosen to assess quality control.</p> <div class="alert alert-info text-left"><b>Isen莽茫o de responsabilidade:</b> Este resumo foi traduzido com recurso a ferramentas de intelig锚ncia artificial e ainda n茫o foi revisto ou verificado.</div> <div class="nav social-icons"> <a class="nav-link w-auto">Compartilhe este artigo</a> <a title="Clique aqui" target="_blank" href="https://www.facebook.com/sharer.php?u=https://portuguese.longdom.org/abstract/modification-of-a-targeted-metabolomics-method-using-hydrophilic-interaction-liquid-chromatographyhigh-resolution-mass-s-105032.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="Clique aqui" target="_blank" href="https://twitter.com/share?url=https://portuguese.longdom.org/abstract/modification-of-a-targeted-metabolomics-method-using-hydrophilic-interaction-liquid-chromatographyhigh-resolution-mass-s-105032.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="Clique aqui" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://portuguese.longdom.org/abstract/modification-of-a-targeted-metabolomics-method-using-hydrophilic-interaction-liquid-chromatographyhigh-resolution-mass-s-105032.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="Clique aqui" target="_blank" href="https://plus.google.com/share?url=https://portuguese.longdom.org/abstract/modification-of-a-targeted-metabolomics-method-using-hydrophilic-interaction-liquid-chromatographyhigh-resolution-mass-s-105032.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Links de conte煤do</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://portuguese.longdom.org/privacy-policy.html" title="Clique aqui">pol铆tica de Privacidade</a></li> <li><a class="" href="https://portuguese.longdom.org/terms-conditions.html" title="Clique aqui">termos e Condi莽玫es</a></li> <li><a class="" href="https://portuguese.longdom.org/authors-reviewers-editors.html" title="Clique aqui">Autores, Revisores e Edi莽茫o</a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Contato Longdom</h4> <p><strong>Grupo Longdom SA</strong><br /> Avenida Roger Vandendriessche,<br /> 18, 1150 Bruxelas, B茅lgica<br /> Telefone: +442038085340<br /> <strong>E-mail:</strong> <a class="white" href="mailto:info@longdom.org" title="Clique aqui">info@longdom.org</a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Conectar</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="Clique aqui" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="Clique aqui" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="Clique aqui" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="Clique aqui" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>direito autoral © 2024 <a href="https://portuguese.longdom.org/" title="Clique aqui" class="white">Longdom Publishing SL</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? 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