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This requires extensive animal experimentation such as to meet regulatory-required proof of efficacy. At present, there are no validated methods of assessing pain in neonatal... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_58551895" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Analgesic products for piglet castration are critically needed. This requires extensive animal experimentation such as to meet regulatory-required proof of efficacy. At present, there are no validated methods of assessing pain in neonatal piglets. This poses challenges for investigators to optimize trial design and to meet ethical obligations to minimize the number of animals needed. Pain in neonatal piglets may be subtle, transient and / or variably expressed and, in the absence of validated methods, investigators must rely on using a range of biochemical, physiological and behavioural variables, many of which appear to have very low (or unknown) sensitivity or specificity for documenting pain, or pain-relieving effects. A previous systematic review of this subject was hampered by the high degree of variability in the literature base both in terms of methods used to assess pain and pain mitigation, as well as in outcomes reported. In this setting we provide a narrative review, to a...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58551895" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f1cc05245eb69cf5cce944bdbdf9648b" rel="nofollow" data-download="{"attachment_id":72909037,"asset_id":58551895,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72909037/download_file?st=MTczMjM4NjA1Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="117217188" href="https://independent.academia.edu/meredithsheil">meredith sheil</a><script data-card-contents-for-user="117217188" type="text/json">{"id":117217188,"first_name":"meredith","last_name":"sheil","domain_name":"independent","page_name":"meredithsheil","display_name":"meredith sheil","profile_url":"https://independent.academia.edu/meredithsheil?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_58551895 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58551895"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58551895, container: ".js-paper-rank-work_58551895", }); });</script></li><li class="js-percentile-work_58551895 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58551895; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_58551895"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_58551895 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="58551895"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58551895; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58551895]").text(description); $(".js-view-count-work_58551895").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_58551895").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="58551895"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i></div><span class="InlineList-item-text u-textTruncate u-pl6x"><a class="InlineList-item-text" data-has-card-for-ri="99234" href="https://www.academia.edu/Documents/in/Animals">Animals</a><script data-card-contents-for-ri="99234" type="text/json">{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (false) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=58551895]'), work: {"id":58551895,"title":"Optimal Methods of Documenting Analgesic Efficacy in Neonatal Piglets Undergoing Castration","created_at":"2021-10-17T02:12:40.534-07:00","url":"https://www.academia.edu/58551895/Optimal_Methods_of_Documenting_Analgesic_Efficacy_in_Neonatal_Piglets_Undergoing_Castration?f_ri=99234","dom_id":"work_58551895","summary":"Analgesic products for piglet castration are critically needed. This requires extensive animal experimentation such as to meet regulatory-required proof of efficacy. At present, there are no validated methods of assessing pain in neonatal piglets. This poses challenges for investigators to optimize trial design and to meet ethical obligations to minimize the number of animals needed. Pain in neonatal piglets may be subtle, transient and / or variably expressed and, in the absence of validated methods, investigators must rely on using a range of biochemical, physiological and behavioural variables, many of which appear to have very low (or unknown) sensitivity or specificity for documenting pain, or pain-relieving effects. A previous systematic review of this subject was hampered by the high degree of variability in the literature base both in terms of methods used to assess pain and pain mitigation, as well as in outcomes reported. 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The hypothesis tested in this study... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18347937" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Long-term success of distraction osteogenesis depends on the ability of the surrounding soft tissues to tolerate distraction forces and to adapt to the resulting increase in skeletal length and volume. The hypothesis tested in this study was that mandibular elongation by distraction induces myocyte proliferation. Unilateral mandibular angle osteotomies were performed in 16 Yucatan minipigs. The hemimandibles were lengthened using semiburied distraction devices (Synthes Maxillofacial, Paoli, PA) with 0-day latency, 1, 2, or 4 mm/d distraction rates, and a neutral fixation period of twice the gap size in days. In 2 additional animals, the dissection and osteotomies were performed, and distraction devices were placed without activation (sham control). At the end of neutral fixation, tissues were taken from masseter muscle overlying the osteotomy and the equivalent region on the unoperated side (contralateral control). Proliferation of myocytes was estimated using immunohistochemical localization with antibodies against proliferating cell nuclear antigen (PCNA). Muscle overlying the distracted mandible showed 6-fold more PCNA-positive myocytes (16.8% +/- 11.3%) than the contralateral control side (2.8% +/- 1.1%, P &amp;lt; .0001). In the 2 sham-control animals, there was a low index of PCNA-positive myocytes on both the osteotomy (2%, 5%) and the contralateral sides (1%, 2%). The results of this study suggest that distraction of the porcine mandible by the protocol described induces myocyte proliferation in the masseter muscle. A proliferative response may contribute to improved long-term stability of mandibular expansion by distraction osteogenesis.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18347937" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fdca690237e5635201e675117ffe536b" rel="nofollow" data-download="{"attachment_id":42175855,"asset_id":18347937,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42175855/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38335397" href="https://independent.academia.edu/LeonardKaban">Leonard Kaban</a><script data-card-contents-for-user="38335397" type="text/json">{"id":38335397,"first_name":"Leonard","last_name":"Kaban","domain_name":"independent","page_name":"LeonardKaban","display_name":"Leonard Kaban","profile_url":"https://independent.academia.edu/LeonardKaban?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_18347937 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="18347937"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 18347937, container: ".js-paper-rank-work_18347937", }); 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The hypothesis tested in this study was that mandibular elongation by distraction induces myocyte proliferation. Unilateral mandibular angle osteotomies were performed in 16 Yucatan minipigs. The hemimandibles were lengthened using semiburied distraction devices (Synthes Maxillofacial, Paoli, PA) with 0-day latency, 1, 2, or 4 mm/d distraction rates, and a neutral fixation period of twice the gap size in days. In 2 additional animals, the dissection and osteotomies were performed, and distraction devices were placed without activation (sham control). At the end of neutral fixation, tissues were taken from masseter muscle overlying the osteotomy and the equivalent region on the unoperated side (contralateral control). Proliferation of myocytes was estimated using immunohistochemical localization with antibodies against proliferating cell nuclear antigen (PCNA). Muscle overlying the distracted mandible showed 6-fold more PCNA-positive myocytes (16.8% +/- 11.3%) than the contralateral control side (2.8% +/- 1.1%, P \u0026amp;lt; .0001). In the 2 sham-control animals, there was a low index of PCNA-positive myocytes on both the osteotomy (2%, 5%) and the contralateral sides (1%, 2%). The results of this study suggest that distraction of the porcine mandible by the protocol described induces myocyte proliferation in the masseter muscle. A proliferative response may contribute to improved long-term stability of mandibular expansion by distraction osteogenesis.","downloadable_attachments":[{"id":42175855,"asset_id":18347937,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38335397,"first_name":"Leonard","last_name":"Kaban","domain_name":"independent","page_name":"LeonardKaban","display_name":"Leonard Kaban","profile_url":"https://independent.academia.edu/LeonardKaban?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=99234","nofollow":false},{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=99234","nofollow":false},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=99234","nofollow":false},{"id":38650,"name":"Cell 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href="https://www.academia.edu/31966396/Scilla_maderensis_TLC_screening_and_positive_inotropic_effect_of_bulb_extracts">Scilla maderensis, TLC screening and positive inotropic effect of bulb extracts</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/31966396" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="81dbf43298f65e2f57d0ab1caf1aa659" rel="nofollow" data-download="{"attachment_id":52240372,"asset_id":31966396,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/52240372/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33372142" href="https://fful.academia.edu/CatarinaDias">Catarina Dias</a><script data-card-contents-for-user="33372142" type="text/json">{"id":33372142,"first_name":"Catarina","last_name":"Dias","domain_name":"fful","page_name":"CatarinaDias","display_name":"Catarina Dias","profile_url":"https://fful.academia.edu/CatarinaDias?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_31966396 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="31966396"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 31966396, container: ".js-paper-rank-work_31966396", }); });</script></li><li class="js-percentile-work_31966396 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 31966396; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_31966396"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_31966396 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="31966396"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 31966396; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=31966396]").text(description); $(".js-view-count-work_31966396").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_31966396").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="31966396"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">19</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="140" href="https://www.academia.edu/Documents/in/Pharmacology">Pharmacology</a>, <script data-card-contents-for-ri="140" type="text/json">{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4083" href="https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine">Complementary and Alternative Medicine</a>, <script data-card-contents-for-ri="4083" type="text/json">{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="14032" href="https://www.academia.edu/Documents/in/Ethnopharmacology">Ethnopharmacology</a><script data-card-contents-for-ri="14032" type="text/json">{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=31966396]'), work: {"id":31966396,"title":"Scilla maderensis, TLC screening and positive inotropic effect of bulb extracts","created_at":"2017-03-21T10:16:05.564-07:00","url":"https://www.academia.edu/31966396/Scilla_maderensis_TLC_screening_and_positive_inotropic_effect_of_bulb_extracts?f_ri=99234","dom_id":"work_31966396","summary":null,"downloadable_attachments":[{"id":52240372,"asset_id":31966396,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33372142,"first_name":"Catarina","last_name":"Dias","domain_name":"fful","page_name":"CatarinaDias","display_name":"Catarina Dias","profile_url":"https://fful.academia.edu/CatarinaDias?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology?f_ri=99234","nofollow":false},{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=99234","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=99234","nofollow":false},{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=99234","nofollow":false},{"id":14080,"name":"Pharmacognosy","url":"https://www.academia.edu/Documents/in/Pharmacognosy?f_ri=99234"},{"id":15019,"name":"Medicinal Plants","url":"https://www.academia.edu/Documents/in/Medicinal_Plants?f_ri=99234"},{"id":44510,"name":"Portugal","url":"https://www.academia.edu/Documents/in/Portugal?f_ri=99234"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro?f_ri=99234"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234"},{"id":131495,"name":"Heart","url":"https://www.academia.edu/Documents/in/Heart?f_ri=99234"},{"id":219827,"name":"Endemic","url":"https://www.academia.edu/Documents/in/Endemic?f_ri=99234"},{"id":309895,"name":"Isolation","url":"https://www.academia.edu/Documents/in/Isolation?f_ri=99234"},{"id":312834,"name":"Cardiovascular system","url":"https://www.academia.edu/Documents/in/Cardiovascular_system?f_ri=99234"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=99234"},{"id":783432,"name":"Biological activity","url":"https://www.academia.edu/Documents/in/Biological_activity?f_ri=99234"},{"id":789968,"name":"Extract","url":"https://www.academia.edu/Documents/in/Extract?f_ri=99234"},{"id":880186,"name":"Vulnerable","url":"https://www.academia.edu/Documents/in/Vulnerable?f_ri=99234"},{"id":888739,"name":"Quntitative Thin Layer Chromatography","url":"https://www.academia.edu/Documents/in/Quntitative_Thin_Layer_Chromatography?f_ri=99234"},{"id":971822,"name":"Active Ingredient","url":"https://www.academia.edu/Documents/in/Active_Ingredient?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21651075" data-work_id="21651075" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21651075/Heart_specific_up_regulation_of_genes_for_B_type_and_C_type_natriuretic_peptide_receptors_in_diabetic_mice">Heart specific up-regulation of genes for B-type and C-type natriuretic peptide receptors in diabetic mice</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21651075" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5f7606cf65a19c4f4c692eeb51e5d7d4" rel="nofollow" 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Christoffersen</a><script data-card-contents-for-user="32490701" type="text/json">{"id":32490701,"first_name":"C.","last_name":"Christoffersen","domain_name":"independent","page_name":"ChristoffersenC","display_name":"C. Christoffersen","profile_url":"https://independent.academia.edu/ChristoffersenC?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21651075 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21651075"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21651075, container: ".js-paper-rank-work_21651075", }); });</script></li><li class="js-percentile-work_21651075 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21651075; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_21651075"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_21651075 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="21651075"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21651075; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21651075]").text(description); $(".js-view-count-work_21651075").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21651075").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21651075"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">10</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="99234" href="https://www.academia.edu/Documents/in/Animals">Animals</a>, <script data-card-contents-for-ri="99234" type="text/json">{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111545" href="https://www.academia.edu/Documents/in/Male">Male</a>, <script data-card-contents-for-ri="111545" type="text/json">{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="116889" href="https://www.academia.edu/Documents/in/Natriuretic_Peptides">Natriuretic Peptides</a><script data-card-contents-for-ri="116889" type="text/json">{"id":116889,"name":"Natriuretic Peptides","url":"https://www.academia.edu/Documents/in/Natriuretic_Peptides?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21651075]'), work: {"id":21651075,"title":"Heart specific up-regulation of genes for B-type and C-type natriuretic peptide receptors in diabetic mice","created_at":"2016-02-07T04:05:34.927-08:00","url":"https://www.academia.edu/21651075/Heart_specific_up_regulation_of_genes_for_B_type_and_C_type_natriuretic_peptide_receptors_in_diabetic_mice?f_ri=99234","dom_id":"work_21651075","summary":null,"downloadable_attachments":[{"id":42292249,"asset_id":21651075,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32490701,"first_name":"C.","last_name":"Christoffersen","domain_name":"independent","page_name":"ChristoffersenC","display_name":"C. Christoffersen","profile_url":"https://independent.academia.edu/ChristoffersenC?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=99234","nofollow":false},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234","nofollow":false},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=99234","nofollow":false},{"id":116889,"name":"Natriuretic Peptides","url":"https://www.academia.edu/Documents/in/Natriuretic_Peptides?f_ri=99234","nofollow":false},{"id":131495,"name":"Heart","url":"https://www.academia.edu/Documents/in/Heart?f_ri=99234"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=99234"},{"id":555007,"name":"Receptor","url":"https://www.academia.edu/Documents/in/Receptor?f_ri=99234"},{"id":915951,"name":"Type 2 Diabetes Mellitus","url":"https://www.academia.edu/Documents/in/Type_2_Diabetes_Mellitus?f_ri=99234"},{"id":1621878,"name":"Guanylate Cyclase","url":"https://www.academia.edu/Documents/in/Guanylate_Cyclase?f_ri=99234"},{"id":2015813,"name":"Atrial Natriuretic Factor","url":"https://www.academia.edu/Documents/in/Atrial_Natriuretic_Factor?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_58007549" data-work_id="58007549" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/58007549/Australian_spiny_mountain_crayfish_and_their_temnocephalan_ectosymbionts_an_ancient_association_on_the_edge_of_coextinction">Australian spiny mountain crayfish and their temnocephalan ectosymbionts: an ancient association on the edge of coextinction?</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Australian spiny mountain crayfish (Euastacus, Parastacidae) and their ecotosymbiotic temnocephalan flatworms (Temnocephalida, Platyhelminthes) may have co-occurred and interacted through deep time, during a period of major environmental... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_58007549" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Australian spiny mountain crayfish (Euastacus, Parastacidae) and their ecotosymbiotic temnocephalan flatworms (Temnocephalida, Platyhelminthes) may have co-occurred and interacted through deep time, during a period of major environmental change. Therefore, reconstructing the history of their association is of evolutionary, ecological, and conservation significance. Here, time-calibrated Bayesian phylogenies of Euastacus species and their temnocephalans (Temnohaswellia and Temnosewellia) indicate near-synchronous diversifications from the Cretaceous. Statistically significant cophylogeny correlations between associated clades suggest linked evolutionary histories. However, there is a stronger signal of codivergence and greater host specificity in Temnosewellia, which co-occurs with Euastacus across its range. Phylogeography and analyses of evolutionary distinctiveness (ED) suggest that regional differences in the impact of climate warming and drying had major effects both on crayfish...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58007549" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ff2e142a83f6348786eb5fcdccd55b76" rel="nofollow" data-download="{"attachment_id":72631713,"asset_id":58007549,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72631713/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="81741437" href="https://independent.academia.edu/LesterCannon">Lester Cannon</a><script data-card-contents-for-user="81741437" type="text/json">{"id":81741437,"first_name":"Lester","last_name":"Cannon","domain_name":"independent","page_name":"LesterCannon","display_name":"Lester Cannon","profile_url":"https://independent.academia.edu/LesterCannon?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_58007549 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58007549"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58007549, container: ".js-paper-rank-work_58007549", }); 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$(".js-view-count[data-work-id=58007549]").text(description); $(".js-view-count-work_58007549").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_58007549").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="58007549"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">14</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2467" href="https://www.academia.edu/Documents/in/Conservation_Biology">Conservation Biology</a>, <script data-card-contents-for-ri="2467" type="text/json">{"id":2467,"name":"Conservation Biology","url":"https://www.academia.edu/Documents/in/Conservation_Biology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4206" href="https://www.academia.edu/Documents/in/Phylogeography">Phylogeography</a>, <script data-card-contents-for-ri="4206" type="text/json">{"id":4206,"name":"Phylogeography","url":"https://www.academia.edu/Documents/in/Phylogeography?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4207" href="https://www.academia.edu/Documents/in/Phylogenetics">Phylogenetics</a>, <script data-card-contents-for-ri="4207" type="text/json">{"id":4207,"name":"Phylogenetics","url":"https://www.academia.edu/Documents/in/Phylogenetics?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6697" href="https://www.academia.edu/Documents/in/Australia">Australia</a><script data-card-contents-for-ri="6697" type="text/json">{"id":6697,"name":"Australia","url":"https://www.academia.edu/Documents/in/Australia?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=58007549]'), work: {"id":58007549,"title":"Australian spiny mountain crayfish and their temnocephalan ectosymbionts: an ancient association on the edge of coextinction?","created_at":"2021-10-14T23:38:51.450-07:00","url":"https://www.academia.edu/58007549/Australian_spiny_mountain_crayfish_and_their_temnocephalan_ectosymbionts_an_ancient_association_on_the_edge_of_coextinction?f_ri=99234","dom_id":"work_58007549","summary":"Australian spiny mountain crayfish (Euastacus, Parastacidae) and their ecotosymbiotic temnocephalan flatworms (Temnocephalida, Platyhelminthes) may have co-occurred and interacted through deep time, during a period of major environmental change. Therefore, reconstructing the history of their association is of evolutionary, ecological, and conservation significance. Here, time-calibrated Bayesian phylogenies of Euastacus species and their temnocephalans (Temnohaswellia and Temnosewellia) indicate near-synchronous diversifications from the Cretaceous. Statistically significant cophylogeny correlations between associated clades suggest linked evolutionary histories. However, there is a stronger signal of codivergence and greater host specificity in Temnosewellia, which co-occurs with Euastacus across its range. Phylogeography and analyses of evolutionary distinctiveness (ED) suggest that regional differences in the impact of climate warming and drying had major effects both on crayfish...","downloadable_attachments":[{"id":72631713,"asset_id":58007549,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":81741437,"first_name":"Lester","last_name":"Cannon","domain_name":"independent","page_name":"LesterCannon","display_name":"Lester Cannon","profile_url":"https://independent.academia.edu/LesterCannon?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2467,"name":"Conservation Biology","url":"https://www.academia.edu/Documents/in/Conservation_Biology?f_ri=99234","nofollow":false},{"id":4206,"name":"Phylogeography","url":"https://www.academia.edu/Documents/in/Phylogeography?f_ri=99234","nofollow":false},{"id":4207,"name":"Phylogenetics","url":"https://www.academia.edu/Documents/in/Phylogenetics?f_ri=99234","nofollow":false},{"id":6697,"name":"Australia","url":"https://www.academia.edu/Documents/in/Australia?f_ri=99234","nofollow":false},{"id":11850,"name":"Crayfish Ecology","url":"https://www.academia.edu/Documents/in/Crayfish_Ecology?f_ri=99234"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=99234"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=99234"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=99234"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234"},{"id":191815,"name":"Biological evolution","url":"https://www.academia.edu/Documents/in/Biological_evolution?f_ri=99234"},{"id":610489,"name":"Temnocephala","url":"https://www.academia.edu/Documents/in/Temnocephala?f_ri=99234"},{"id":611756,"name":"Turbellaria","url":"https://www.academia.edu/Documents/in/Turbellaria?f_ri=99234"},{"id":880279,"name":"Bayes Theorem","url":"https://www.academia.edu/Documents/in/Bayes_Theorem-1?f_ri=99234"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=99234"}]}, }) } 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$(".js-view-count[data-work-id=34351459]").text(description); $(".js-view-count-work_34351459").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_34351459").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="34351459"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">14</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="622" href="https://www.academia.edu/Documents/in/Nephrology">Nephrology</a>, <script data-card-contents-for-ri="622" type="text/json">{"id":622,"name":"Nephrology","url":"https://www.academia.edu/Documents/in/Nephrology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="653" href="https://www.academia.edu/Documents/in/Urology">Urology</a>, <script data-card-contents-for-ri="653" type="text/json">{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="65171" href="https://www.academia.edu/Documents/in/Smooth_muscle">Smooth muscle</a><script data-card-contents-for-ri="65171" type="text/json">{"id":65171,"name":"Smooth muscle","url":"https://www.academia.edu/Documents/in/Smooth_muscle?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=34351459]'), work: {"id":34351459,"title":"Characteristics of Spontaneous Activity in the Bladder Trigone","created_at":"2017-08-25T06:17:04.430-07:00","url":"https://www.academia.edu/34351459/Characteristics_of_Spontaneous_Activity_in_the_Bladder_Trigone?f_ri=99234","dom_id":"work_34351459","summary":null,"downloadable_attachments":[{"id":54243546,"asset_id":34351459,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":10264,"first_name":"Rasheda","last_name":"Chowdhury","domain_name":"imperial","page_name":"RashedaChowdhury","display_name":"Rasheda Chowdhury","profile_url":"https://imperial.academia.edu/RashedaChowdhury?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":622,"name":"Nephrology","url":"https://www.academia.edu/Documents/in/Nephrology?f_ri=99234","nofollow":false},{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=99234","nofollow":false},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=99234","nofollow":false},{"id":65171,"name":"Smooth muscle","url":"https://www.academia.edu/Documents/in/Smooth_muscle?f_ri=99234","nofollow":false},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=99234"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=99234"},{"id":419061,"name":"Urinary System","url":"https://www.academia.edu/Documents/in/Urinary_System?f_ri=99234"},{"id":485556,"name":"Guinea Pig","url":"https://www.academia.edu/Documents/in/Guinea_Pig?f_ri=99234"},{"id":556021,"name":"Muscle contraction","url":"https://www.academia.edu/Documents/in/Muscle_contraction?f_ri=99234"},{"id":633766,"name":"Sediment Characteristic","url":"https://www.academia.edu/Documents/in/Sediment_Characteristic?f_ri=99234"},{"id":783432,"name":"Biological activity","url":"https://www.academia.edu/Documents/in/Biological_activity?f_ri=99234"},{"id":1902592,"name":"Urinary Bladder","url":"https://www.academia.edu/Documents/in/Urinary_Bladder?f_ri=99234"},{"id":2111545,"name":"Spontaneous Activity","url":"https://www.academia.edu/Documents/in/Spontaneous_Activity?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15432569 coauthored" data-work_id="15432569" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15432569/New_understanding_of_the_molecular_mechanism_of_receptor_mediated_genomic_actions_of_the_vitamin_D_hormone">New understanding of the molecular mechanism of receptor-mediated genomic actions of the vitamin D hormone</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15432569" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/67560764/Reconceiving_autoimmunity_An_overview">Reconceiving autoimmunity: An overview</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Three interconnected positions are advocated: (1) although serving as a useful model, the immune self does not exist as such; (2) instead of a self/nonself demarcation, the immune system &#39;sees&#39; itself, i.e., it does not ignore the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_67560764" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Three interconnected positions are advocated: (1) although serving as a useful model, the immune self does not exist as such; (2) instead of a self/nonself demarcation, the immune system &#39;sees&#39; itself, i.e., it does not ignore the &#39;self&#39; or attack the &#39;other;&#39; but exhibits a spectrum of responses, which when viewed from outside the system appear as discrimination of &#39;self&#39; and &#39;nonself&#39; based on certain criteria of reactivity. When immune reactions are conceived in terms of normal physiology and open exchange with the environment, where borders dividing host and foreign are elusive and changing, host defense is only part of the immune system&#39;s functions, which actually comprise two basic tasks: protection, i.e., to preserve host integrity, and maintenance of organismic identity. And thus (3) if the spectrum of immunity is enlarged, differentiating low reactive &#39;autoimmune&#39; reactions from activated immune responses against the &#39;...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/67560764" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a2c98b2391d78b677729d4265c72a523" rel="nofollow" data-download="{"attachment_id":78331518,"asset_id":67560764,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78331518/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="8781609" href="https://bu.academia.edu/AlfredTauber">Alfred Tauber</a><script data-card-contents-for-user="8781609" type="text/json">{"id":8781609,"first_name":"Alfred","last_name":"Tauber","domain_name":"bu","page_name":"AlfredTauber","display_name":"Alfred Tauber","profile_url":"https://bu.academia.edu/AlfredTauber?f_ri=99234","photo":"https://0.academia-photos.com/8781609/3222050/3791806/s65_alfred.tauber.jpg"}</script></span></span></li><li class="js-paper-rank-work_67560764 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="67560764"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 67560764, container: ".js-paper-rank-work_67560764", }); 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$(".js-view-count[data-work-id=67560764]").text(description); $(".js-view-count-work_67560764").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_67560764").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="67560764"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">13</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="7043" href="https://www.academia.edu/Documents/in/Symbiosis">Symbiosis</a>, <script data-card-contents-for-ri="7043" type="text/json">{"id":7043,"name":"Symbiosis","url":"https://www.academia.edu/Documents/in/Symbiosis?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9846" href="https://www.academia.edu/Documents/in/Ecology">Ecology</a>, <script data-card-contents-for-ri="9846" type="text/json">{"id":9846,"name":"Ecology","url":"https://www.academia.edu/Documents/in/Ecology?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12320" href="https://www.academia.edu/Documents/in/Autoimmunity">Autoimmunity</a><script data-card-contents-for-ri="12320" type="text/json">{"id":12320,"name":"Autoimmunity","url":"https://www.academia.edu/Documents/in/Autoimmunity?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=67560764]'), work: {"id":67560764,"title":"Reconceiving autoimmunity: An overview","created_at":"2022-01-07T13:45:30.710-08:00","url":"https://www.academia.edu/67560764/Reconceiving_autoimmunity_An_overview?f_ri=99234","dom_id":"work_67560764","summary":"Three interconnected positions are advocated: (1) although serving as a useful model, the immune self does not exist as such; (2) instead of a self/nonself demarcation, the immune system \u0026#39;sees\u0026#39; itself, i.e., it does not ignore the \u0026#39;self\u0026#39; or attack the \u0026#39;other;\u0026#39; but exhibits a spectrum of responses, which when viewed from outside the system appear as discrimination of \u0026#39;self\u0026#39; and \u0026#39;nonself\u0026#39; based on certain criteria of reactivity. When immune reactions are conceived in terms of normal physiology and open exchange with the environment, where borders dividing host and foreign are elusive and changing, host defense is only part of the immune system\u0026#39;s functions, which actually comprise two basic tasks: protection, i.e., to preserve host integrity, and maintenance of organismic identity. And thus (3) if the spectrum of immunity is enlarged, differentiating low reactive \u0026#39;autoimmune\u0026#39; reactions from activated immune responses against the \u0026#39;...","downloadable_attachments":[{"id":78331518,"asset_id":67560764,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":8781609,"first_name":"Alfred","last_name":"Tauber","domain_name":"bu","page_name":"AlfredTauber","display_name":"Alfred Tauber","profile_url":"https://bu.academia.edu/AlfredTauber?f_ri=99234","photo":"https://0.academia-photos.com/8781609/3222050/3791806/s65_alfred.tauber.jpg"}],"research_interests":[{"id":7043,"name":"Symbiosis","url":"https://www.academia.edu/Documents/in/Symbiosis?f_ri=99234","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=99234","nofollow":false},{"id":9846,"name":"Ecology","url":"https://www.academia.edu/Documents/in/Ecology?f_ri=99234","nofollow":false},{"id":12320,"name":"Autoimmunity","url":"https://www.academia.edu/Documents/in/Autoimmunity?f_ri=99234","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=99234"},{"id":26977,"name":"Theoretical biology","url":"https://www.academia.edu/Documents/in/Theoretical_biology?f_ri=99234"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=99234"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=99234"},{"id":71304,"name":"Autoimmune diseases","url":"https://www.academia.edu/Documents/in/Autoimmune_diseases?f_ri=99234"},{"id":80414,"name":"Mathematical Sciences","url":"https://www.academia.edu/Documents/in/Mathematical_Sciences?f_ri=99234"},{"id":84924,"name":"Immunity","url":"https://www.academia.edu/Documents/in/Immunity?f_ri=99234"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234"},{"id":324154,"name":"Immune system","url":"https://www.academia.edu/Documents/in/Immune_system?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21350563 coauthored" data-work_id="21350563" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21350563/Split_sample_comparison_of_directional_and_liquid_nitrogen_vapour_freezing_method_on_post_thaw_semen_quality_in_white_rhinoceroses_Ceratotherium_simum_simum_and_Ceratotherium_simum_cottoni_">Split-sample comparison of directional and liquid nitrogen vapour freezing method on post-thaw semen quality in white rhinoceroses (Ceratotherium simum simum and Ceratotherium simum cottoni)</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21350563" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" 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data-has-card-for-user="42440253" href="https://independent.academia.edu/CatherineReid2">Catherine Reid</a><script data-card-contents-for-user="42440253" type="text/json">{"id":42440253,"first_name":"Catherine","last_name":"Reid","domain_name":"independent","page_name":"CatherineReid2","display_name":"Catherine Reid","profile_url":"https://independent.academia.edu/CatherineReid2?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-21350563">+1</span><div class="hidden js-additional-users-21350563"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/FrankGoeritz">Frank Goeritz</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-21350563'), placement: 'bottom', hide_delay: 200, html: true, 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container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_21350563 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="21350563"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21350563; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21350563]").text(description); $(".js-view-count-work_21350563").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21350563").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div 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href="https://www.academia.edu/Documents/in/Endangered_Species">Endangered Species</a>, <script data-card-contents-for-ri="23979" type="text/json">{"id":23979,"name":"Endangered Species","url":"https://www.academia.edu/Documents/in/Endangered_Species?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="43820" href="https://www.academia.edu/Documents/in/Theriogenology">Theriogenology</a><script data-card-contents-for-ri="43820" type="text/json">{"id":43820,"name":"Theriogenology","url":"https://www.academia.edu/Documents/in/Theriogenology?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21350563]'), work: {"id":21350563,"title":"Split-sample comparison of directional and liquid nitrogen vapour freezing method on post-thaw semen quality in white rhinoceroses (Ceratotherium simum simum and Ceratotherium simum cottoni)","created_at":"2016-01-31T18:54:18.756-08:00","url":"https://www.academia.edu/21350563/Split_sample_comparison_of_directional_and_liquid_nitrogen_vapour_freezing_method_on_post_thaw_semen_quality_in_white_rhinoceroses_Ceratotherium_simum_simum_and_Ceratotherium_simum_cottoni_?f_ri=99234","dom_id":"work_21350563","summary":null,"downloadable_attachments":[{"id":41828431,"asset_id":21350563,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42440253,"first_name":"Catherine","last_name":"Reid","domain_name":"independent","page_name":"CatherineReid2","display_name":"Catherine Reid","profile_url":"https://independent.academia.edu/CatherineReid2?f_ri=99234","photo":"/images/s65_no_pic.png"},{"id":42515286,"first_name":"Frank","last_name":"Goeritz","domain_name":"independent","page_name":"FrankGoeritz","display_name":"Frank Goeritz","profile_url":"https://independent.academia.edu/FrankGoeritz?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4559,"name":"Reproduction","url":"https://www.academia.edu/Documents/in/Reproduction?f_ri=99234","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=99234","nofollow":false},{"id":23979,"name":"Endangered Species","url":"https://www.academia.edu/Documents/in/Endangered_Species?f_ri=99234","nofollow":false},{"id":43820,"name":"Theriogenology","url":"https://www.academia.edu/Documents/in/Theriogenology?f_ri=99234","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=99234"},{"id":77509,"name":"Perissodactyla","url":"https://www.academia.edu/Documents/in/Perissodactyla?f_ri=99234"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=99234"},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=99234"},{"id":151662,"name":"Additives","url":"https://www.academia.edu/Documents/in/Additives?f_ri=99234"},{"id":160656,"name":"Potassium","url":"https://www.academia.edu/Documents/in/Potassium?f_ri=99234"},{"id":277066,"name":"Head","url":"https://www.academia.edu/Documents/in/Head?f_ri=99234"},{"id":945101,"name":"Liquid Nitrogen","url":"https://www.academia.edu/Documents/in/Liquid_Nitrogen?f_ri=99234"},{"id":1029221,"name":"Freezing","url":"https://www.academia.edu/Documents/in/Freezing?f_ri=99234"},{"id":1043331,"name":"Semen Preservation","url":"https://www.academia.edu/Documents/in/Semen_Preservation?f_ri=99234"},{"id":1221081,"name":"Semen Quality","url":"https://www.academia.edu/Documents/in/Semen_Quality?f_ri=99234"},{"id":1223743,"name":"Sperm Quality","url":"https://www.academia.edu/Documents/in/Sperm_Quality?f_ri=99234"},{"id":1242344,"name":"Plasma Membrane","url":"https://www.academia.edu/Documents/in/Plasma_Membrane?f_ri=99234"},{"id":1759332,"name":"Sexual reproduction","url":"https://www.academia.edu/Documents/in/Sexual_reproduction?f_ri=99234"},{"id":2295414,"name":"Thermal Gradient","url":"https://www.academia.edu/Documents/in/Thermal_Gradient?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14424536 coauthored" data-work_id="14424536" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14424536/Crystallization_scale_preparation_of_a_stable_GPCR_signaling_complex_between_constitutively_active_rhodopsin_and_G_protein">Crystallization scale preparation of a stable GPCR signaling complex between constitutively active rhodopsin and G-protein</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The activation of the G-protein transducin (Gt) by rhodopsin (Rho) has been intensively studied for several decades. It is the best understood example of GPCR activation mechanism and serves as a template for other GPCRs. The structure of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14424536" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The activation of the G-protein transducin (Gt) by rhodopsin (Rho) has been intensively studied for several decades. It is the best understood example of GPCR activation mechanism and serves as a template for other GPCRs. The structure of the Rho/G protein complex, which is transiently formed during the signaling reaction, is of particular interest. It can help understanding the molecular details of how retinal isomerization leads to the G protein activation, as well as shed some light on how GPCR recognizes its cognate G protein. The native Rho/Gt complex isolated from bovine retina suffers from low stability and loss of the retinal ligand. Recently, we reported that constitutively active mutant of rhodopsin E113Q forms a Rho/Gt complex that is stable in detergent solution. Here, we introduce methods for a large scale preparation of the complex formed by the thermo-stabilized and constitutively active rhodopsin mutant N2C/M257Y/D282C(RhoM257Y) and the native Gt purified from bovine...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14424536" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5d061468a027f7985d3e91f8b859ca25" rel="nofollow" data-download="{"attachment_id":44157456,"asset_id":14424536,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44157456/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33365072" href="https://psi-ch.academia.edu/GebhardSchertler">Gebhard F X Schertler</a><script data-card-contents-for-user="33365072" type="text/json">{"id":33365072,"first_name":"Gebhard","last_name":"Schertler","domain_name":"psi-ch","page_name":"GebhardSchertler","display_name":"Gebhard F X Schertler","profile_url":"https://psi-ch.academia.edu/GebhardSchertler?f_ri=99234","photo":"https://0.academia-photos.com/33365072/12447282/13852903/s65_gebhard.schertler.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-14424536">+3</span><div class="hidden js-additional-users-14424536"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/DmitryVeprintsev">Dmitry Veprintsev</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://stanford.academia.edu/ShojiMaeda">Shoji Maeda</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://unibasel.academia.edu/MichaelHennig">Michael Hennig</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-14424536'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-14424536').html(); 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It is the best understood example of GPCR activation mechanism and serves as a template for other GPCRs. The structure of the Rho/G protein complex, which is transiently formed during the signaling reaction, is of particular interest. It can help understanding the molecular details of how retinal isomerization leads to the G protein activation, as well as shed some light on how GPCR recognizes its cognate G protein. The native Rho/Gt complex isolated from bovine retina suffers from low stability and loss of the retinal ligand. Recently, we reported that constitutively active mutant of rhodopsin E113Q forms a Rho/Gt complex that is stable in detergent solution. Here, we introduce methods for a large scale preparation of the complex formed by the thermo-stabilized and constitutively active rhodopsin mutant N2C/M257Y/D282C(RhoM257Y) and the native Gt purified from bovine...","downloadable_attachments":[{"id":44157456,"asset_id":14424536,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33365072,"first_name":"Gebhard","last_name":"Schertler","domain_name":"psi-ch","page_name":"GebhardSchertler","display_name":"Gebhard F X Schertler","profile_url":"https://psi-ch.academia.edu/GebhardSchertler?f_ri=99234","photo":"https://0.academia-photos.com/33365072/12447282/13852903/s65_gebhard.schertler.jpg"},{"id":33412293,"first_name":"Dmitry","last_name":"Veprintsev","domain_name":"independent","page_name":"DmitryVeprintsev","display_name":"Dmitry 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examining possible acoustic otofunctional effects on the cochlear... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6177129" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The purpose of this paper is to present the experimental device and the work in progress performed in search for objective organic correlation of damage to hearing, examining possible acoustic otofunctional effects on the cochlear epithelium of the rat due to exposure to microwaves (900 MHz). Two experiments using male Sprague-Dawley rats were carried out with a far-field exposure in a cubic chamber. No statistically significant evidence was obtained at both specific absorption rate (SAR) values. The exposure system and the diagnostic apparatus are extremely useful to investigate a potential effect on the auditory system: however, with the parameters applied in these experiments, no evidence was observed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6177129" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="530cceebc2a15c891f82c8ce1a48cb0d" rel="nofollow" data-download="{"attachment_id":48982557,"asset_id":6177129,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48982557/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="9413374" href="https://independent.academia.edu/MartaCristal">Marta Cristal</a><script data-card-contents-for-user="9413374" type="text/json">{"id":9413374,"first_name":"Marta","last_name":"Cristal","domain_name":"independent","page_name":"MartaCristal","display_name":"Marta Cristal","profile_url":"https://independent.academia.edu/MartaCristal?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_6177129 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6177129"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6177129, container: ".js-paper-rank-work_6177129", }); 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Medicine","url":"https://www.academia.edu/Documents/in/Chinese_Herbal_Medicine?f_ri=99234"},{"id":424295,"name":"Survival Rate","url":"https://www.academia.edu/Documents/in/Survival_Rate?f_ri=99234"},{"id":469033,"name":"Radiation Injuries","url":"https://www.academia.edu/Documents/in/Radiation_Injuries?f_ri=99234"},{"id":1159983,"name":"Radiation Tolerance","url":"https://www.academia.edu/Documents/in/Radiation_Tolerance?f_ri=99234"},{"id":1281407,"name":"Whole Body","url":"https://www.academia.edu/Documents/in/Whole_Body?f_ri=99234"},{"id":1427023,"name":"Bone Marrow Failure","url":"https://www.academia.edu/Documents/in/Bone_Marrow_Failure?f_ri=99234"},{"id":1979328,"name":"Total body irradiation","url":"https://www.academia.edu/Documents/in/Total_body_irradiation?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16800989" data-work_id="16800989" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16800989/Topical_and_mucosal_liposomes_for_vaccine_delivery">Topical and mucosal liposomes for vaccine delivery</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Mucosal (and in minor extent transcutanous) stimulation can induce local or distant mucosa secretory IgA. Liposomes and other vesicles as mucosal and transcutaneous adjuvants are attractive alternatives to parenteral vaccination.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16800989" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Mucosal (and in minor extent transcutanous) stimulation can induce local or distant mucosa secretory IgA. Liposomes and other vesicles as mucosal and transcutaneous adjuvants are attractive alternatives to parenteral vaccination. Liposomes can be massively produced under good manufacturing practices and stored for long periods, at high antigen/vesicle mass ratios. However, their uptake by antigen-presenting cells (APC) at the inductive sites remains as a major challenge. As neurotoxicity is a major concern in intranasal delivery, complexes between archaeosomes and calcium as well as cationic liposomes complexed with plasmids encoding for antigenic proteins could safely elicit secretory and systemic antigen-specific immune responses. Oral bilosomes generate intense immune responses that remain to be tested against challenge, but the admixing with toxins or derivatives is mandatory to reduce the amount of antigen. Most of the current experimental designs, however, underestimate the mu...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16800989" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2045af17158cd3a65abfe8885eaefcab" rel="nofollow" data-download="{"attachment_id":39190188,"asset_id":16800989,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39190188/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10555545" href="https://independent.academia.edu/mariajosemorilla">maria jose morilla</a><script data-card-contents-for-user="10555545" type="text/json">{"id":10555545,"first_name":"maria jose","last_name":"morilla","domain_name":"independent","page_name":"mariajosemorilla","display_name":"maria jose morilla","profile_url":"https://independent.academia.edu/mariajosemorilla?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_16800989 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16800989"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16800989, container: ".js-paper-rank-work_16800989", }); 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$(".js-view-count[data-work-id=16800989]").text(description); $(".js-view-count-work_16800989").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16800989").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16800989"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">13</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4365" href="https://www.academia.edu/Documents/in/Vaccines">Vaccines</a>, <script data-card-contents-for-ri="4365" type="text/json">{"id":4365,"name":"Vaccines","url":"https://www.academia.edu/Documents/in/Vaccines?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="8501" href="https://www.academia.edu/Documents/in/Phospholipids">Phospholipids</a>, <script data-card-contents-for-ri="8501" type="text/json">{"id":8501,"name":"Phospholipids","url":"https://www.academia.edu/Documents/in/Phospholipids?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9334" href="https://www.academia.edu/Documents/in/Inflammation">Inflammation</a>, <script data-card-contents-for-ri="9334" type="text/json">{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a><script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16800989]'), work: {"id":16800989,"title":"Topical and mucosal liposomes for vaccine delivery","created_at":"2015-10-14T16:14:17.535-07:00","url":"https://www.academia.edu/16800989/Topical_and_mucosal_liposomes_for_vaccine_delivery?f_ri=99234","dom_id":"work_16800989","summary":"Mucosal (and in minor extent transcutanous) stimulation can induce local or distant mucosa secretory IgA. Liposomes and other vesicles as mucosal and transcutaneous adjuvants are attractive alternatives to parenteral vaccination. Liposomes can be massively produced under good manufacturing practices and stored for long periods, at high antigen/vesicle mass ratios. However, their uptake by antigen-presenting cells (APC) at the inductive sites remains as a major challenge. As neurotoxicity is a major concern in intranasal delivery, complexes between archaeosomes and calcium as well as cationic liposomes complexed with plasmids encoding for antigenic proteins could safely elicit secretory and systemic antigen-specific immune responses. Oral bilosomes generate intense immune responses that remain to be tested against challenge, but the admixing with toxins or derivatives is mandatory to reduce the amount of antigen. Most of the current experimental designs, however, underestimate the mu...","downloadable_attachments":[{"id":39190188,"asset_id":16800989,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":10555545,"first_name":"maria jose","last_name":"morilla","domain_name":"independent","page_name":"mariajosemorilla","display_name":"maria jose morilla","profile_url":"https://independent.academia.edu/mariajosemorilla?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4365,"name":"Vaccines","url":"https://www.academia.edu/Documents/in/Vaccines?f_ri=99234","nofollow":false},{"id":8501,"name":"Phospholipids","url":"https://www.academia.edu/Documents/in/Phospholipids?f_ri=99234","nofollow":false},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=99234","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=99234","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=99234"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234"},{"id":147066,"name":"Liposomes","url":"https://www.academia.edu/Documents/in/Liposomes?f_ri=99234"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems?f_ri=99234"},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids?f_ri=99234"},{"id":209510,"name":"Epidermis","url":"https://www.academia.edu/Documents/in/Epidermis?f_ri=99234"},{"id":375078,"name":"Topical Drug Administration","url":"https://www.academia.edu/Documents/in/Topical_Drug_Administration?f_ri=99234"},{"id":648676,"name":"Mucus","url":"https://www.academia.edu/Documents/in/Mucus?f_ri=99234"},{"id":954248,"name":"Detergents","url":"https://www.academia.edu/Documents/in/Detergents?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_32635794" data-work_id="32635794" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/32635794/Epigenetics_and_its_role_in_male_infertility">Epigenetics and its role in male infertility</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32635794" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="688e62ea6833868d6066e93b41f045c3" rel="nofollow" data-download="{"attachment_id":52806149,"asset_id":32635794,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/52806149/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="63327258" href="https://independent.academia.edu/manojkumar1991">manoj kumar</a><script data-card-contents-for-user="63327258" type="text/json">{"id":63327258,"first_name":"manoj","last_name":"kumar","domain_name":"independent","page_name":"manojkumar1991","display_name":"manoj kumar","profile_url":"https://independent.academia.edu/manojkumar1991?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_32635794 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="32635794"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 32635794, container: ".js-paper-rank-work_32635794", }); 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$(".js-view-count[data-work-id=32635794]").text(description); $(".js-view-count-work_32635794").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_32635794").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="32635794"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">9</a> </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="156" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="75577" href="https://www.academia.edu/Documents/in/Spermatogenesis">Spermatogenesis</a>, <script data-card-contents-for-ri="75577" type="text/json">{"id":75577,"name":"Spermatogenesis","url":"https://www.academia.edu/Documents/in/Spermatogenesis?f_ri=99234","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="99234" href="https://www.academia.edu/Documents/in/Animals">Animals</a><script data-card-contents-for-ri="99234" type="text/json">{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32635794]'), work: {"id":32635794,"title":"Epigenetics and its role in male infertility","created_at":"2017-04-25T00:32:10.448-07:00","url":"https://www.academia.edu/32635794/Epigenetics_and_its_role_in_male_infertility?f_ri=99234","dom_id":"work_32635794","summary":null,"downloadable_attachments":[{"id":52806149,"asset_id":32635794,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":63327258,"first_name":"manoj","last_name":"kumar","domain_name":"independent","page_name":"manojkumar1991","display_name":"manoj kumar","profile_url":"https://independent.academia.edu/manojkumar1991?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=99234","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=99234","nofollow":false},{"id":75577,"name":"Spermatogenesis","url":"https://www.academia.edu/Documents/in/Spermatogenesis?f_ri=99234","nofollow":false},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=99234","nofollow":false},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=99234"},{"id":178928,"name":"Male Infertility","url":"https://www.academia.edu/Documents/in/Male_Infertility?f_ri=99234"},{"id":352300,"name":"Embryonic Development","url":"https://www.academia.edu/Documents/in/Embryonic_Development?f_ri=99234"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=99234"},{"id":2562986,"name":"Oligospermia","url":"https://www.academia.edu/Documents/in/Oligospermia?f_ri=99234"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16100688 coauthored" data-work_id="16100688" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16100688/Exercise_and_time_dependent_benefits_to_learning_and_memory">Exercise and time-dependent benefits to learning and memory</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">While it is well established that exercise can improve cognitive performance, it is unclear how long these benefits endure after exercise has ended. Accordingly, the effects of voluntary exercise on cognitive function and brain-derived... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16100688" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">While it is well established that exercise can improve cognitive performance, it is unclear how long these benefits endure after exercise has ended. Accordingly, the effects of voluntary exercise on cognitive function and brain-derived neurotrophic factor (BDNF) protein levels, a major player in the mechanisms governing the dynamics of memory formation and storage, were assessed immediately after a 3-week running period, or after a 1-week or 2-week delay following the exercise period. All exercised mice showed improved performance on the radial arm water maze relative to sedentary animals. Unexpectedly, fastest acquisition (fewest errors and shortest latency) occurred in animals trained following a 1-week delay, while best memory performance in the probe trial was observed in those trained immediately after the exercise period. Assessment of the time course of hippocampal BDNF availability following exercise revealed significant elevations of BDNF immediately after the exercise peri...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16100688" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c673a8e25ca8aa3fa163401986293e51" rel="nofollow" data-download="{"attachment_id":42748779,"asset_id":16100688,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42748779/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35219708" href="https://independent.academia.edu/NCastello">N. 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Accordingly, the effects of voluntary exercise on cognitive function and brain-derived neurotrophic factor (BDNF) protein levels, a major player in the mechanisms governing the dynamics of memory formation and storage, were assessed immediately after a 3-week running period, or after a 1-week or 2-week delay following the exercise period. All exercised mice showed improved performance on the radial arm water maze relative to sedentary animals. Unexpectedly, fastest acquisition (fewest errors and shortest latency) occurred in animals trained following a 1-week delay, while best memory performance in the probe trial was observed in those trained immediately after the exercise period. Assessment of the time course of hippocampal BDNF availability following exercise revealed significant elevations of BDNF immediately after the exercise peri...","downloadable_attachments":[{"id":42748779,"asset_id":16100688,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35219708,"first_name":"N.","last_name":"Castello","domain_name":"independent","page_name":"NCastello","display_name":"N. Castello","profile_url":"https://independent.academia.edu/NCastello?f_ri=99234","photo":"/images/s65_no_pic.png"},{"id":60799202,"first_name":"N.","last_name":"Berchtold","domain_name":"independent","page_name":"NBerchtold","display_name":"N. 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Recently, the skin has emerged as a potential alternative route... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_11180030" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Non-invasive vaccine delivery is a top priority for public health agencies because conventional immunization practices are unsafe and associated with numerous limitations. Recently, the skin has emerged as a potential alternative route for non-invasive delivery of vaccine. Topical immunization (TI), introduction of antigen through topical application onto the intact skin, has many practical merits compared to injectable routes of administration. One of the possibilities for increasing the penetration of bioactives through the skin is the use of vesicular systems. Specially designed lipid vesicles are attracting intense attention and can be used for non-invasive antigen delivery. In the present study, elastic vesicle transfersomes, non-ionic surfactant vesicles (niosomes) and liposomes were used to study their relative potential in non-invasive delivery of tetanus toxoid (TT). Transfersomes, niosomes and liposomes were prepared and characterized for shape, size and entrapment efficiency. These vesicles were extruded through polycarbonate filter (50-nm pore size) to assess the elasticity of the vesicles. The immune stimulating activity of transfersomes, niosomes and liposomes were studied by measuring the serum anti-TT IgG titre following topical immunization. The immune response elicited by topical immunization was compared with that elicited by same dose of alum-adsorbed tetanus toxoid (AATT) given intramuscularly. The results indicate that optimal formulations of transfersomes, niosomes and liposomes could entrap 72.7 ± 3.4, 42.5 ± 2.4 and 41.3 ± 2.2% of antigen and their elasticity values were 124.4 ± 4.2, 29.3 ± 2.4 and 21.7 ± 1.9, respectively. In vivo study revealed that topically given TT containing transfersomes, after secondary immunization, could elicit immune response (anti-TT-IgG) that was equivalent to one that produced following intramuscularly alum-adsorbed TT-based immunization. In comparison to transfersomes, niosomes and liposomes elicited weaker immune response. Thus transfersomes hold promise for effective non-invasive topical delivery of antigen(s).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/11180030" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f8ce882259efc785721e63af8472c42b" rel="nofollow" data-download="{"attachment_id":46839333,"asset_id":11180030,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46839333/download_file?st=MTczMjM4NjA1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="26997969" href="https://independent.academia.edu/VivekMishra44">Vivek Mishra</a><script data-card-contents-for-user="26997969" type="text/json">{"id":26997969,"first_name":"Vivek","last_name":"Mishra","domain_name":"independent","page_name":"VivekMishra44","display_name":"Vivek Mishra","profile_url":"https://independent.academia.edu/VivekMishra44?f_ri=99234","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_11180030 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="11180030"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 11180030, container: ".js-paper-rank-work_11180030", }); 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Recently, the skin has emerged as a potential alternative route for non-invasive delivery of vaccine. Topical immunization (TI), introduction of antigen through topical application onto the intact skin, has many practical merits compared to injectable routes of administration. One of the possibilities for increasing the penetration of bioactives through the skin is the use of vesicular systems. Specially designed lipid vesicles are attracting intense attention and can be used for non-invasive antigen delivery. In the present study, elastic vesicle transfersomes, non-ionic surfactant vesicles (niosomes) and liposomes were used to study their relative potential in non-invasive delivery of tetanus toxoid (TT). Transfersomes, niosomes and liposomes were prepared and characterized for shape, size and entrapment efficiency. These vesicles were extruded through polycarbonate filter (50-nm pore size) to assess the elasticity of the vesicles. The immune stimulating activity of transfersomes, niosomes and liposomes were studied by measuring the serum anti-TT IgG titre following topical immunization. The immune response elicited by topical immunization was compared with that elicited by same dose of alum-adsorbed tetanus toxoid (AATT) given intramuscularly. The results indicate that optimal formulations of transfersomes, niosomes and liposomes could entrap 72.7 ± 3.4, 42.5 ± 2.4 and 41.3 ± 2.2% of antigen and their elasticity values were 124.4 ± 4.2, 29.3 ± 2.4 and 21.7 ± 1.9, respectively. In vivo study revealed that topically given TT containing transfersomes, after secondary immunization, could elicit immune response (anti-TT-IgG) that was equivalent to one that produced following intramuscularly alum-adsorbed TT-based immunization. In comparison to transfersomes, niosomes and liposomes elicited weaker immune response. Thus transfersomes hold promise for effective non-invasive topical delivery of antigen(s).","downloadable_attachments":[{"id":46839333,"asset_id":11180030,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":26997969,"first_name":"Vivek","last_name":"Mishra","domain_name":"independent","page_name":"VivekMishra44","display_name":"Vivek Mishra","profile_url":"https://independent.academia.edu/VivekMishra44?f_ri=99234","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4365,"name":"Vaccines","url":"https://www.academia.edu/Documents/in/Vaccines?f_ri=99234","nofollow":false},{"id":23890,"name":"Comparative 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