Primer Design with Oligo Primer Analysis Software v. 7

<HTML> <HEAD> <link rel="shortcut icon" href="http://www.oligo.net/graphics/olicon.gif"> <TITLE>Primer Design with Oligo Primer Analysis Software v. 7</TITLE> <meta Name="keywords" Content="primer selection software tool, PCR software, Oligo 7 program, batch processing PCR primer analysis, sequencing primers design, primer design software, probe design software, consensus primers, multiplex PCR primers, site directed mutagenesis, back translation, reverse translation, open reading frame analysis, siRNA design, real time PCR"> <meta Name="description" Content="OLIGO performs a range of functions for researchers in PCR and related technologies, enables to design consensus, multiplex and degenerate primers. Reverse translation, restriction enzyme and open reading frames analysis, oligonucleotide database, primer secondary structure, LCR, siRNA, molecular beacons and nested primers design, real time PCR, batch file processing."> <link href="mbi.css" rel="stylesheet" type="text/css"> <style type="text/css">  </style> </HEAD>  <body background="/graphics/olibkg.jpg"> <div id="fb-root"></div> <script>(function(d, s, id) { var js, fjs = d.getElementsByTagName(s)[0]; if (d.getElementById(id)) return; js = d.createElement(s); js.id = id; js.src = "//connect.facebook.net/en_US/all.js#xfbml=1"; fjs.parentNode.insertBefore(js, fjs); }(document, 'script', 'facebook-jssdk'));</script> <div align="left"><img src="graphics/oligonet.gif" alt="MBI header" width="800" height="150" border="0" usemap="#Map"> <map name="Map"> <area shape="rect" coords="228,100,322,123" href="tutorials.html" alt="Tutorials"> <area shape="rect" coords="513,100,616,125" href="ordering.html" alt="Order Oligo"> <area shape="rect" coords="645,100,764,124" href="contact.html"> <area shape="rect" coords="362,99,482,124" href="downloads.html"> </map> </div> <table width="800" border="1" cellpadding="10" cellspacing="1" bgcolor="#FFFFFF"> <tr> <td width="320" class="centerit"><p align="center">OLIGO<br> 1267 Vondelpark, Colorado Springs, CO 80907<br> 719 684 7988</p> <p align="center"><img src="graphics/new-interface.gif" alt="new interface" width="320" height="500"></p> <p align="center"><span class="small"> According to Soft-Go, independent reviewers, Oligo exceeds quality standards. <br> </span> <a>  </a><a href="http://www.soft-go.com/view/Oligo_59570.html" target="_blank"> <img src="graphics/soft-go.gif" alt="Oligo Award" width="92" height="55"></a> </p> <p><font face="Arial, Helvetica, sans-serif"><a href="new_features.pdf">Oligo 7 New Features</a></font> </p> </p> <p>  </p></td> <td width="470"><p align="justify" class="style4 style7"><FONT COLOR="#FF0000"><B>OLIGO</B></FONT> Primer Analysis Software is the essential tool for designing and analyzing sequencing and PCR primers, synthetic genes, and various kinds of probes including siRNA and molecular beacons. <br> Based on the most up-to date nearest neighbor thermodynamic data, Oligo's search algorithms find optimal primers for PCR, including TaqMan, highly multiplexed, consensus or degenerate primers.  Multiple file batch processing is possible. It is also an invaluable tool for site directed mutagenesis. <br> For each primer or primer pair, Oligo's various analysis windows show a multitude of useful data, such as DNA and RNA secondary structure, dimer formation, false priming and homology, internal stability, composition and physical properties.  With Oligo you can analyze open reading frames down to predicted molecular weight and pKa of proteins, and search for restriction enzyme sites, not only in DNA but also in reverse-translated proteins.<br> The first version of the software appeared on the market in 1989. It went through several modifications, and the last one, the change from version 6 to 7, was the most comprehensive. Oligo 7 can automatically select multiplex primers, process sequence files in batch modes, automatically design PCR primers to cover multiple DNA regions in just one search and automatically find primer/probe sets for real time PCR or finds nested primers sets. Oligo search protocols (scoring system) may be customized in detail, so you may optimize the results according to your specific needs. <br> Oligo runs on Macintosh and Windows and it may be downloaded from this site. Click on the Tutorials link above to see Oligo 7 general overview and the major windows screen designs.</p> <p align="justify" class="style4 style7"><FONT COLOR="#FF0000"><B>OLIGO'</B></FONT>s comprehensive functionality matches several other commercial primer design applications put together, so you can design probes for high throughput analysis, multiplex them or focus on one set of primers/probes and optimize it for a new, not yet published application.</p> <p align="justify" class="style4 style7">Unlike other vendors who license software for a certain period of time, Oligo is supported as long as you use it. We still help customers who use Oligo 5, released in the mid 90's. Our pricing and ordering form are given on the <a href="ordering.html">Ordering</a> page.</p> <p align="justify" class="style4 style7"><a href="oligo7presentation.pps">Click for the presentation:<img src="graphics/oligoppt.gif" alt="Power Point Presentation Download" width="150" height="112" align="right"></a></p> </td> </tr> </table> <div align="center"><span class="normal style2"><FONT FACE="Arial, Helvetica, sans-serif">© 1998 -</FONT></span> <span class="normal style2"><FONT FACE="Arial, Helvetica, sans-serif">20</FONT></span><span class="style6"><FONT FACE="Arial, Helvetica, sans-serif">24</FONT></span><span class="normal style2"><FONT FACE="Arial, Helvetica, sans-serif"> Molecular Biology Insights, Inc. All rights reserved.</FONT></span></div> </BODY> </HTML>

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Primer Design with Oligo Primer Analysis Software v. 7