eCM Journal - Manuscript - Three-dimensional culture model to study the biology of vacuolated notochordal cells from mouse nucleus pulposus explants -True Open Access

<!doctype html> <html> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">  <title>eCM Journal - Manuscript - Three-dimensional culture model to study the biology of vacuolated notochordal cells from mouse nucleus pulposus explants -True Open Access</title>  <meta NAME="keywords" CONTENT="journal, journals, ecmjournal, cells, cell, materials, material, ecm, eur, euro, matter, mat, european, open free, access, Graub眉nden, Schweiz, Literature, Courses, Cases, Craniomaxillofacial, Spine, Veterinary, education, fractures, progenitors, stem cell, regenerative medicine, musculoskeletal development, connective tissues, progenitor cell, progenitors, orthopedic, trauma, spine, dental, AOTrauma, stromal, stem cells, msc, mscs, not for protfit, donat"> <meta NAME="description" CONTENT="European Cells & Materials Journal - Open and Free Access - The Official Research Journal of AOCMF, AOTrauma, European Orthopaedic Research Society (EORS), Swiss Society for Biomaterials (SSB) and Tissue & Cell Engineering Society (TCES)"> <meta NAME="robots" CONTENT="index,follow"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-width, initial-scale=1"> <script language="JavaScript" src="../../scripts/scripts.js" type="text/javascript"></script> <link href="../../css/multiColumnTemplate.css" rel="stylesheet" type="text/css">    </head> <body> <div class="container"> <header> <div class="primary_header"> <table width="100%" border="0" cellpadding="0" cellspacing="0"> <tbody> <tr> <td width="300"><a href="../../index.html"><img src="../../images-design/ecm-journal-logo-2016.jpg" alt="eCM (Eur Cell Mater / e Cells & Materials)" width="250" height="52" title="eCM (Eur Cell Mater / e Cells & Materials)"/></a></td> <td align="left" valign="middle" class="logotext">eCM Open Access Scientific Journal</td> </tr> </tbody> </table> </div> <div class="primary_header2">  ISSN:1473-2262         NLM:100973416 (<a href="https://www.ncbi.nlm.nih.gov/nlmcatalog/100973416" target="_blank">link</a>)         DOI:10.22203/eCM</div> <nav class="secondary_header" id="menu"> <ul> <li><a href="/index.html">Home</a></li> <li><a href="/archive.html">Issues</a></li> <li><a href="/scope.php">Scope</a></li> <li><a href="/instructions_to_authors.html">Submit Paper</a></li> <li><a href="/search_ecm_abstract.html">Paper search</a></li> </ul> </nav> </header> <section> <article class="left_article"> <h3><b>2023   Volume No 45  pages 72-87</b> </h3>  <table border="0" cellpadding="0" cellspacing="0" width="100%"> <tr> <td valign="top" width="100%" height="28"><p><b> Title: </b>Three-dimensional culture model to study the biology of vacuolated notochordal cells from mouse nucleus pulposus explants</p></td> </tr> <tr> <td valign="top" width="100%" height="29"><p><b>Authors: </b><b></b> L Paillat, K Coutant, M Dutilleul, S Le Lay, A Camus</p></td> </tr> <tr> <td valign="top" width="100%" height="33"><p><b> Address: </b>Nantes Université, Oniris, CHU Nantes, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-4400 Nantes, France</p></td> </tr> <tr> <td valign="top" height="43"><p><strong>E-mail: </strong>anne.camus <strong>at</strong> univ-nantes.fr<br> </p></td> </tr> <tr> <td valign="top" width="100%" height="229"><p><b> <img src="/papers/vol045/graphical abstract/vol045a06.jpg" alt="" width="858" height="229" class="placeholder"/></b> <p><b>Abstract: </b>Intervertebral disc degeneration (IDD) involves cellular changes in the nucleus pulposus (NP) characterised by a decline of the large vacuolated notochordal cells (vNCs) and a rise of smaller vacuole-free mature chondrocyte-like NP cells. An increasing number of studies demonstrate that notochordal cells (NCs) exert disease-modifying effects, establishing that NC-secreted factors are essential for the maintenance of a healthy intervertebral disc (IVD). However, understanding the role of the NCs is hampered by a restricted reserve of native cells and the lack of robust ex vivo cell model. A precise dissection enabled the isolation of NP cells from 4 d post-natal stage mouse spines and their culture into self-organised micromasses. The maintenance of cells’ phenotypic characteristics was demonstrated by the presence of intracytoplasmic vacuoles and the immuno-colocalisation of the NC-markers (brachyury; SOX9) after 9 d of culture both in hypoxic and normoxic conditions. A significant increase of the size of the micromass was observed under hypoxia, consistent with a higher level of Ki-67+ immunostained proliferative cells. Furthermore, several proteins of interest for the study of vNCs phenotype (CD44; caveolin-1; aquaporin 2; patched-1) were successfully detected at the plasma membrane of NP-cells cultured in micromasses under hypoxic condition. IHC was performed on mouse IVD sections as control staining. An innovative 3D culture model of vNCs derived from mouse postnatal NP is proposed, allowing future ex vivo exploration of their basic biology and of the signalling pathways involved in IVD homeostasis that may be relevant for disc repair.<br> </td> </tr> <tr> <td valign="top" height="43"><p><strong>Keywords</strong>: Intervertebral disc, nucleus pulposus cells, vacuolated notochordal cell, ex vivo mouse tissue, three-dimensional culture system, hypoxia, tissue engineering.<br> </p></td> </tr> <tr> <td valign="top" height="43"><p><b>Publication date: </b>March 3rd 2023</p></td> </tr> <tr> <td valign="top" width="100%" height="43"><p><b>Article download:</b> <b><a href="/papers/vol045/pdf/v045a06.pdf">Pages 72-87</a></b> (PDF file)<strong><br> DOI:</strong> 10.22203/eCM.v045a06</p></td> </tr> </table> </table> </td> <a href="https://twitter.com/share?url=http://www.ecmjournal.org/papers/vol045/vol045a06.php" target="_blank"> <img src="../../images/logos/share logos/twitter.png" alt="Twitter" width="40" height="35 " /> </a>  <a href="http://www.facebook.com/sharer.php?u=http://www.ecmjournal.org/papers/vol045/vol045a06.php" target="_blank"> <img src="../../images/logos/share logos/facebook.png" alt="Facebook" width="40" height="35" /> </a>  <a href="https://plus.google.com/share?url=http://www.ecmjournal.org/papers/vol045/vol045a06.php" target="_blank"> <img src="../../images/logos/share logos/google.png" alt="Google"width="40" height="35" /> </a>  <a href="http://www.linkedin.com/shareArticle?mini=true&url=http://www.ecmjournal.org/papers/vol045/vol045a06.php" target="_blank"> <img src="../../images/logos/share logos/linkedin.png" alt="LinkedIn" width="40" height="35"/> </a>  <a href="javascript:;" onclick="window.print()"> <img src="../../images/logos/share logos/print.png" alt="Print"width="40" height="35" /> </a> </tr> </table> <p> </p>  </article> <aside class="right_article"><br> <ul> <a href="../../info.php"> <li>Info on eCM</li></a> <a href="../../editors.html"><li>Editors</li></a> <a href="../../official_societies.html"><li>Official Societies</li></a> <a href="../../Privacy.html"> <li>Privacy Policy</li></a> <a href="../../contact.html"> <li>Contact</li></a> </ul><br> <div class="googlesearch"> <script> (function() { var cx = '005753416099071425706:8n5j25f8dju'; var gcse = document.createElement('script'); gcse.type = 'text/javascript'; gcse.async = true; gcse.src = 'https://cse.google.com/cse.js?cx=' + cx; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(gcse, s); })(); </script> <gcse:search linkTarget="_parent"></gcse:search> </div> <br> <br> </aside> </section> <footer class="secondary_footer"> <div class="copyright"> ©Open Access / Author retains copyright (CC BY) / <a href="https://forummmpub.com/" target="new">Forum Multimedia Publishing LLC.</a>, Part of <a href="https://www.imrpress.com/" target="new">IMR Press</a>.</div> </footer> </div> </body> </html>

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eCM Journal - Manuscript - Three-dimensional culture model to study the biology of vacuolated notochordal cells from mouse nucleus pulposus explants -True Open Access