Yeast Display Library Screening Services

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Our excellent biopanning strategies and state-of-the-art equipment enable us to provide the best solutions. Our scientists tailor the most appropriate strategies to screen our customers' libraries effectively. </p> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Background</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Services</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Advantages</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Applications</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">Case</span> </div> <script> window.addEventListener('load',function(){ page_tab_margin() page_tab_css(); })</script> <h2 class="h2_tit page_tab_margin" id="tab1"> What is Yeast Display? </h2> <p> Yeast display is a cutting-edge technology used to present proteins, peptides, or antibody fragments on the surface of yeast cells. This powerful technique enables the high-throughput screening of large libraries to identify variants with optimal properties such as high binding affinity and specificity. </p> <p> <strong>Yeast Display Library Screening</strong> </p> <p> By coupling yeast antibody display libraries with fluorescence-activated cell sorting (FACS), high-affinity antibodies that bind specifically to targeted antigens can be selected using labeled target antigens. This combination provides a more controlled and quantitative selection process, enhancing the enrichment of clones with specific binding properties more effectively than phage display. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Yeast Display Library Screening Services - Rapid Identification of High-Affinity Binders </h2> <p> Creative Biolabs' yeast display screening services are designed to provide comprehensive and customized solutions for the discovery and characterization of high-affinity, specific binders to meet your specific research and development needs. Our services can significantly advance research, diagnostics, and therapeutics by leveraging advanced screening technologies and expert knowledge in yeast display systems. </p> <p> <strong>Workflow</strong> </p> <p class="ServiceShowPic"> <img loading="lazy" src="../static/img/1-1-2-2-yeast-display-library-screening-services-1.jpg" alt="Fig. 1 Workflow of yeast display library screening. (Creative Biolabs Original)" width="780" height="425" /> </p> <ul class="ullist"> <li> MACS-Based Screening: Magnetic-activated cell sorting (MACS) is employed in yeast display library initial screening to enrich populations of yeast cells that display proteins with specific binding characteristics. </li> <li> FACS-Based Sorting: Utilizing high-throughput screening techniques such as Fluorescence-Activated Cell Sorting (FACS), we isolate yeast cells displaying binders with desired affinities and specificities from our yeast display libraries, ensuring high-quality outcomes. </li> <li> Selective Isolation: We can enrich populations based on binding characteristics, speeding up the identification of high-affinity binders. </li> </ul> <br /> <p> <strong>Related Services Available</strong> </p> <ul class="ullist"> <li> <a href="/Yeast-Display-Human-scFv-Library.html">Premade Human Antibody Library for Yeast Display</a> </li> <li> <a href="/identification-of-posttranslational-modification-dependent-protein-interactions-using-yeast-display.html">Identification of Post-translational Modification-Dependent Protein Interactions</a> </li> <li> Premade Yeast Display Single Domain Antibody Library Screening </li> <li> Premade Yeast Display Single Chain Antibody Library Screening </li> <li> Yeast Display Immune Antibody Library Screening </li> <li> Yeast Display Antibody Library Screening for Cell-based Target </li> <li> Mutant Epitope Specific Antibody Screening from Yeast Display Library </li> <li> TCR-Like Antibody Screening from Yeast Display Library </li> <li> Anti-GPCR Antibody Screening from Yeast Display Library </li> <li> Yeast Display Library Screening for pH-dependent Antibody </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab3"> Advantages of Yeast Display Library Screening for Binder Discovery </h2> <div class="row"> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> Yeast Display Platform</strong> <div class="featurescont"> Continually improving our yeast display platform ensuring proper folding and post-translational modifications of displayed proteins to provide cutting-edge solutions. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> Wide Range of Targets</strong> <div class="featurescont"> Screen against various targets, including small molecules, proteins, peptides, and cells. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> High-throughput and Quantitative Screening</strong> <div class="featurescont"> High-throughput techniques for rapidly screening millions of yeast cells, facilitate the efficient identification of high-affinity binders. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> Rapid Screening Cycles</strong> <div class="featurescont"> Relatively fast screening and enriching high-affinity binders, allowing for the rapid identification and characterization of potential binders. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> Characterization</strong> <div class="featurescont"> Characterize binders through yeast surface titrations and DNA sequencing, providing detailed insights into binding affinity and specificity. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> Integration with Other Methods</strong> <div class="featurescont"> Combination with other selection methods, such as MACS and phage display, to further enhance the selection process and identify the best candidates for binder development. </div> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab4"> Applications </h2> <div class="row"> <div class="col-md-6"> <ul class="ullist"> <li> Antibody discovery. </li> <li> Affinity maturation. </li> <li> Humanization. </li> <li> Targeting membrane proteins. </li> <li> Screening against challenging targets. </li> </ul> </div> <div class="col-md-6"> <ul class="ullist"> <li> Creating novel constructs such as ADCs and bispecific antibodies. </li> <li> Identify enzyme inhibitor. </li> <li> Map epitopes on antigens. </li> <li> Discover/Validate new therapeutic targets. </li> <li> Discover interaction between ligand and receptor. </li> </ul> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab5"> Cases </h2> <p align="center"> <strong>Yeast Display Antibody Library Screening Against Target Protein</strong> </p> <p align="center"> <strong>Round 1 MACS Screening</strong> </p> <p class="ServiceShowPic"> <img loading="lazy" src="../static/img/1-1-2-2-yeast-display-library-screening-services-2.jpg" alt="Fig. 1 Workflow of yeast display library screening. (Creative Biolabs Original)" width="550" height="458" /> </p> <p> MACS-based initial screening is conducted. We performed tests of scFv display and the target protein binding for output cells by FACS after screening. The staining signals of the display tag are good, and a positive enrichment can be observed for the target protein with the binding signal >20%. The enriched pools also had a strong binding signal to the control protein. </p> <p align="center"> <strong>Round 2 FACS Sorting</strong> </p> <p class="ServiceShowPic"> <img loading="lazy" src="../static/img/1-1-2-2-yeast-display-library-screening-services-3.jpg" alt="Fig. 2 Round one of yeast display antibody library screening. (Creative Biolabs Original)" width="680" height="358" /> </p> <p> FACS Sorting against the target protein is performed and competed with a control protein with sequence similarity of more than 90% to the target protein. The results showed a weak positive signal in the target protein group. The clones from R3 Gate G (5.30%) are randomly picked and performed binding tests and positive clones are continued with DNA sequencing. </p> <p> <span class="inquiry-button">Contact</span> our team of experts to find out more about our Yeast Display Services. </p><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. 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