Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta

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However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes blaCTX-M, blaIMP, and blaTEM conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in blaCTX-M and blaTEM copy numbers and a mean increase in blaIMP copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta." > <link rel="image_src" href="https://pub.mdpi-res.com/img/journals/microorganisms-logo.png?8600e93ff98dbf14" > <meta name="dc.title" content="Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta"> <meta name="dc.creator" content="Katharina A. Werner"> <meta name="dc.creator" content="Lara Feyen"> <meta name="dc.creator" content="Tobias Hübner"> <meta name="dc.creator" content="Nicolas Brüggemann"> <meta name="dc.creator" content="Katharina Prost"> <meta name="dc.creator" content="Elisabeth Grohmann"> <meta name="dc.type" content="Communication"> <meta name="dc.source" content="Microorganisms 2023, Vol. 11, Page 308"> <meta name="dc.date" content="2023-01-24"> <meta name ="dc.identifier" content="10.3390/microorganisms11020308"> <meta name="dc.publisher" content="Multidisciplinary Digital Publishing Institute"> <meta name="dc.rights" content="http://creativecommons.org/licenses/by/3.0/"> <meta name="dc.format" content="application/pdf" > <meta name="dc.language" content="en" > <meta name="dc.description" content="Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes blaCTX-M, blaIMP, and blaTEM conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in blaCTX-M and blaTEM copy numbers and a mean increase in blaIMP copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta." > <meta name="dc.subject" content="ecological sanitation" > <meta name="dc.subject" content="compost" > <meta name="dc.subject" content="antibiotic resistance" > <meta name="dc.subject" content="horizontal gene transfer" > <meta name="dc.subject" content="qPCR" > <meta name ="prism.issn" content="2076-2607"> <meta name ="prism.publicationName" content="Microorganisms"> <meta name ="prism.publicationDate" content="2023-01-24"> <meta name ="prism.volume" content="11"> <meta name ="prism.number" content="2"> <meta name ="prism.section" content="Communication" > <meta name ="prism.startingPage" content="308" > <meta name="citation_issn" content="2076-2607"> <meta name="citation_journal_title" content="Microorganisms"> <meta name="citation_publisher" content="Multidisciplinary Digital Publishing Institute"> <meta name="citation_title" content="Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta"> <meta name="citation_publication_date" content="2023/2"> <meta name="citation_online_date" content="2023/01/24"> <meta name="citation_volume" content="11"> <meta name="citation_issue" content="2"> <meta name="citation_firstpage" content="308"> <meta name="citation_author" content="Werner, Katharina A."> <meta name="citation_author" content="Feyen, Lara"> <meta name="citation_author" content="Hübner, Tobias"> <meta name="citation_author" content="Brüggemann, Nicolas"> <meta name="citation_author" content="Prost, Katharina"> <meta name="citation_author" content="Grohmann, Elisabeth"> <meta name="citation_doi" content="10.3390/microorganisms11020308"> <meta name="citation_id" content="mdpi-microorganisms11020308"> <meta name="citation_abstract_html_url" content="https://www.mdpi.com/2076-2607/11/2/308"> <meta name="citation_pdf_url" content="https://www.mdpi.com/2076-2607/11/2/308/pdf?version=1676021057"> <link rel="alternate" type="application/pdf" title="PDF Full-Text" href="https://www.mdpi.com/2076-2607/11/2/308/pdf?version=1676021057"> <meta name="fulltext_pdf" content="https://www.mdpi.com/2076-2607/11/2/308/pdf?version=1676021057"> <meta name="citation_fulltext_html_url" content="https://www.mdpi.com/2076-2607/11/2/308/htm"> <link rel="alternate" type="text/html" title="HTML Full-Text" href="https://www.mdpi.com/2076-2607/11/2/308/htm"> <meta name="fulltext_html" content="https://www.mdpi.com/2076-2607/11/2/308/htm"> <link rel="alternate" type="text/xml" title="XML Full-Text" href="https://www.mdpi.com/2076-2607/11/2/308/xml"> <meta name="fulltext_xml" content="https://www.mdpi.com/2076-2607/11/2/308/xml"> <meta name="citation_xml_url" content="https://www.mdpi.com/2076-2607/11/2/308/xml"> <meta name="twitter:card" content="summary" /> <meta name="twitter:site" content="@MDPIOpenAccess" /> <meta name="twitter:image" content="https://pub.mdpi-res.com/img/journals/microorganisms-logo-social.png?8600e93ff98dbf14" /> <meta property="fb:app_id" content="131189377574"/> <meta property="og:site_name" content="MDPI"/> <meta property="og:type" content="article"/> <meta property="og:url" content="https://www.mdpi.com/2076-2607/11/2/308" /> <meta property="og:title" content="Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta" /> <meta property="og:description" content="Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes blaCTX-M, blaIMP, and blaTEM conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in blaCTX-M and blaTEM copy numbers and a mean increase in blaIMP copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. 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Werner</div><div id="profile-card-drop10340303" data-dropdown-content class="f-dropdown content profile-card-content" aria-hidden="true" tabindex="-1"><div class="profile-card__title"><div class="sciprofiles-link" style="display: inline-block"><div class="sciprofiles-link__link"><img class="sciprofiles-link__image" src="/bundles/mdpisciprofileslink/img/unknown-user.png" style="width: auto; height: 16px; border-radius: 50%;"><span class="sciprofiles-link__name">Katharina A. 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max-width: 13px !important; height: auto; top: -5px;"></a>, </span><span class="inlineblock "><div class='profile-card-drop' data-dropdown='profile-card-drop10340304' data-options='is_hover:true, hover_timeout:5000'> Lara Feyen</div><div id="profile-card-drop10340304" data-dropdown-content class="f-dropdown content profile-card-content" aria-hidden="true" tabindex="-1"><div class="profile-card__title"><div class="sciprofiles-link" style="display: inline-block"><div class="sciprofiles-link__link"><img class="sciprofiles-link__image" src="/bundles/mdpisciprofileslink/img/unknown-user.png" style="width: auto; height: 16px; border-radius: 50%;"><span class="sciprofiles-link__name">Lara Feyen</span></div></div></div><div class="profile-card__buttons" style="margin-bottom: 10px;"><a href="https://sciprofiles.com/profile/author/d20zQWZZVitkcWdEMlNrVDRPdXl2SU85UlNwMlJVRUdyUkMxbXozd2ZNbz0=?utm_source=mdpi.com&utm_medium=website&utm_campaign=avatar_name" class="button button--color-inversed" target="_blank"> SciProfiles </a><a href="https://scilit.com/scholars?q=Lara%20Feyen" class="button button--color-inversed" target="_blank"> Scilit </a><a href="https://www.preprints.org/search?search1=Lara%20Feyen&field1=authors" class="button button--color-inversed" target="_blank"> Preprints.org </a><a href="https://scholar.google.com/scholar?q=Lara%20Feyen" class="button button--color-inversed" target="_blank" rels="noopener noreferrer"> Google Scholar </a></div></div><sup> 1</sup>, </span><span class="inlineblock "><div class='profile-card-drop' data-dropdown='profile-card-drop10340305' data-options='is_hover:true, hover_timeout:5000'> Tobias Hübner</div><div id="profile-card-drop10340305" data-dropdown-content class="f-dropdown content profile-card-content" aria-hidden="true" tabindex="-1"><div class="profile-card__title"><div class="sciprofiles-link" style="display: inline-block"><div class="sciprofiles-link__link"><img class="sciprofiles-link__image" src="/bundles/mdpisciprofileslink/img/unknown-user.png" style="width: auto; 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width: 13px; margin-left: 3px; max-width: 13px !important; height: auto; top: -5px;"></a> and </span><span class="inlineblock "><div class='profile-card-drop' data-dropdown='profile-card-drop10340308' data-options='is_hover:true, hover_timeout:5000'> Elisabeth Grohmann</div><div id="profile-card-drop10340308" data-dropdown-content class="f-dropdown content profile-card-content" aria-hidden="true" tabindex="-1"><div class="profile-card__title"><div class="sciprofiles-link" style="display: inline-block"><div class="sciprofiles-link__link"><img class="sciprofiles-link__image" src="/profiles/1688605/thumb/Elisabeth_Grohmann.png" style="width: auto; height: 16px; border-radius: 50%;"><span class="sciprofiles-link__name">Elisabeth Grohmann</span></div></div></div><div class="profile-card__buttons" style="margin-bottom: 10px;"><a href="https://sciprofiles.com/profile/1688605?utm_source=mdpi.com&utm_medium=website&utm_campaign=avatar_name" class="button button--color-inversed" target="_blank"> SciProfiles </a><a href="https://scilit.com/scholars?q=Elisabeth%20Grohmann" class="button button--color-inversed" target="_blank"> Scilit </a><a href="https://www.preprints.org/search?search1=Elisabeth%20Grohmann&field1=authors" class="button button--color-inversed" target="_blank"> Preprints.org </a><a href="https://scholar.google.com/scholar?q=Elisabeth%20Grohmann" class="button button--color-inversed" target="_blank" rels="noopener noreferrer"> Google Scholar </a></div></div><sup> 1,*</sup><span style="display: inline; margin-left: 5px;"></span><a class="toEncode emailCaptcha visibility-hidden" data-author-id="10340308" href="/cdn-cgi/l/email-protection#b798d4d9d39ad4d0de98db98d2dad6dedb9ac7c5d8c3d2d4c3ded8d994878787858182868087d687d3878f878387d587d58582878087d38686838f878087878680878e87d487d583d587868787"><sup><i class="fa fa-envelope-o"></i></sup></a></span> </div> <div class="nrm"></div> <span style="display:block; height:6px;"></span> <div></div> <div style="margin: 5px 0 15px 0;" class="hypothesis_container"> <div class="art-affiliations"> <div class="affiliation "> <div class="affiliation-item"><sup>1</sup></div> <div class="affiliation-name ">Faculty of Life Sciences and Technology, Department of Microbiology, Berliner Hochschule für Technik, 13347 Berlin, Germany</div> </div> <div class="affiliation "> <div class="affiliation-item"><sup>2</sup></div> <div class="affiliation-name ">Department of Environmental Microbiology, Helmholtz Centre for Environmental Research GmbH—Umweltforschungszentrum Leipzig (UFZ), 04318 Leipzig, Germany</div> </div> <div class="affiliation "> <div class="affiliation-item"><sup>3</sup></div> <div class="affiliation-name ">Institute of Bio- and Geosciences (Agrosphere, IBG-3), Forschungszentrum Jülich, 52425 Jülich, Germany</div> </div> <div class="affiliation"> <div class="affiliation-item"><sup>*</sup></div> <div class="affiliation-name ">Author to whom correspondence should be addressed. </div> </div> </div> </div> <div class="bib-identity" style="margin-bottom: 10px;"> <em>Microorganisms</em> <b>2023</b>, <em>11</em>(2), 308; <a href="https://doi.org/10.3390/microorganisms11020308">https://doi.org/10.3390/microorganisms11020308</a> </div> <div class="pubhistory" style="font-weight: bold; padding-bottom: 10px;"> <span style="display: inline-block">Submission received: 19 December 2022</span> / <span style="display: inline-block">Revised: 17 January 2023</span> / <span style="display: inline-block">Accepted: 22 January 2023</span> / <span style="display: inline-block">Published: 24 January 2023</span> </div> <div class="belongsTo" style="margin-bottom: 10px;"> (This article belongs to the Special Issue <a href=" /journal/microorganisms/special_issues/multidrug_resistant_bacteria_mechanisms ">Multidrug-Resistant Bacteria in the Environment, Their Resistance and Transfer Mechanisms</a>)<br/> </div> <div class="highlight-box1"> <div class="download"> <a class="button button--color-inversed button--drop-down" data-dropdown="drop-download-1045721" aria-controls="drop-supplementary-1045721" aria-expanded="false"> Download <i class="material-icons">keyboard_arrow_down</i> </a> <div id="drop-download-1045721" class="f-dropdown label__btn__dropdown label__btn__dropdown--button" data-dropdown-content aria-hidden="true" tabindex="-1"> <a class="UD_ArticlePDF" href="/2076-2607/11/2/308/pdf?version=1676021057" data-name="Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta" data-journal="microorganisms">Download PDF</a> <br/> <a id="js-pdf-with-cover-access-captcha" href="#" data-target="/2076-2607/11/2/308/pdf-with-cover" class="accessCaptcha">Download PDF with Cover</a> <br/> <a id="js-xml-access-captcha" href="#" data-target="/2076-2607/11/2/308/xml" class="accessCaptcha">Download XML</a> <br/> <a href="/2076-2607/11/2/308/epub" id="epub_link">Download Epub</a> <br/> </div> <div class="js-browse-figures" style="display: inline-block;"> <a href="#" class="button button--color-inversed margin-bottom-10 openpopupgallery UI_BrowseArticleFigures" data-target='article-popup' data-counterslink = "https://www.mdpi.com/2076-2607/11/2/308/browse" >Browse Figures</a> </div> <div id="article-popup" class="popupgallery" style="display: inline; line-height: 200%"> <a href="https://pub.mdpi-res.com/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001.png?1676021131" title=" <strong>Figure 1</strong><br/> <p>Copy numbers of beta-lactam ARGs <span class="html-italic">bla<sub>IMP</sub></span> (<b>A</b>,<b>B</b>) and <span class="html-italic">bla<sub>CTX-M</sub></span> (<b>C</b>,<b>D</b>), as well as <span class="html-italic">bla<sub>TEM</sub></span> (<b>E</b>,<b>F</b>) as assessed by qPCR. Copy numbers were normalized to 16S rRNA gene copy numbers (left charts) and to 1 g compost (right charts). Bars represent the average of three individual qPCR measurements with standard deviation. Start samples, 14-days samples (only repetition 1) and end samples are depicted individually for the first (E1, E1-B) and second repetition of composting (E2, E2-B). End samples comprise replicates from front (end 1), middle (end 2) and back (end 3) of each compost pile. The <span class="html-italic">p</span>-values were calculated to compare start with end samples of composting for all four compost piles combined (average of the triplicate end samples, no differentiation of the treatments). None of the sample sets showed significance (<span class="html-italic">p</span> &lt; 0.05). Therefore, they are indicated by “n.s.” (not significant).</p> "> </a> <a href="https://pub.mdpi-res.com/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002.png?1676021128" title=" <strong>Figure 2</strong><br/> <p>Copy numbers of the class 1 integron-integrase gene <span class="html-italic">intI1</span> (<b>A</b>,<b>B</b>) and IncP-1 marker gene <span class="html-italic">korB</span> (<b>C</b>,<b>D</b>) as assessed by qPCR. Copy numbers were normalized to 16S rRNA gene copy numbers (left charts) and to 1 g compost (right charts). Bars represent the average of three individual qPCR measurements with the standard deviation. Start samples, 14-days samples (only repetition 1) and end samples are depicted individually for the first (E1, E1-B) and second repetition of composting (E2, E2-B). End samples comprise replicates from the front (end 1), middle (end 2) and back (end 3) of each compost pile. Asterisks indicate the <span class="html-italic">p</span>-values obtained from Student’s <span class="html-italic">t</span>-test (* <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01) representing the statistical significance of the data. The <span class="html-italic">p</span>-values were calculated to compare start with end samples of composting for all four compost piles combined (average of the triplicate end samples, no differentiation of the treatments).</p> "> </a> </div> <a class="button button--color-inversed" href="/2076-2607/11/2/308/review_report">Review Reports</a> <a class="button button--color-inversed" href="/2076-2607/11/2/308/notes">Versions Notes</a> </div> </div> <div class="responsive-moving-container small hidden" data-id="article-counters" style="margin-top: 15px;"></div> <div class="html-dynamic"> <section> <div class="art-abstract art-abstract-new in-tab hypothesis_container"> <p> <div><section class="html-abstract" id="html-abstract"> <h2 id="html-abstract-title">Abstract</h2><b>:</b> <div class="html-p">Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes <span class="html-italic">bla<sub>CTX-M</sub></span>, <span class="html-italic">bla<sub>IMP</sub></span>, and <span class="html-italic">bla<sub>TEM</sub></span> conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, <span class="html-italic">intI1</span> and <span class="html-italic">korB</span>, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in <span class="html-italic">bla<sub>CTX-M</sub></span> and <span class="html-italic">bla<sub>TEM</sub></span> copy numbers and a mean increase in <span class="html-italic">bla<sub>IMP</sub></span> copy numbers. The decrease in both <span class="html-italic">intI1</span> and <span class="html-italic">korB</span> genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta.</div> </section> <div id="html-keywords"> <div class="html-gwd-group"><div id="html-keywords-title">Keywords: </div><a href="/search?q=ecological+sanitation">ecological sanitation</a>; <a href="/search?q=compost">compost</a>; <a href="/search?q=antibiotic+resistance">antibiotic resistance</a>; <a href="/search?q=horizontal+gene+transfer">horizontal gene transfer</a>; <a href="/search?q=qPCR">qPCR</a></div> <div> </div> </div> </div> </p> </div> </section> </div> <div class="hypothesis_container"> <ul class="menu html-nav" data-prev-node="#html-quick-links-title"> </ul> <div class="html-body"> <section id='sec1-microorganisms-11-00308' type='intro'><h2 data-nested='1'> 1. Introduction</h2><div class='html-p'>Human excrements contain large amounts of nutrients required for plant nutrition, such as phosphorus and nitrogen [<a href="#B1-microorganisms-11-00308" class="html-bibr">1</a>], which remain mostly unused in countries using water-based sanitation. Ecological sanitation via thermophilic composting can serve as a treatment to convert human excreta into a humus-rich fertilizer [<a href="#B2-microorganisms-11-00308" class="html-bibr">2</a>]. Thermophilic composting is an aerobic and exothermic degradation process of organic material mediated by microorganisms. A first mesophilic phase with temperatures between ambient and 45 °C is followed by a thermophilic phase with temperatures above 45 °C, a second mesophilic, and finally a cooling or maturation phase. Predominant microbial species change over the process [<a href="#B3-microorganisms-11-00308" class="html-bibr">3</a>,<a href="#B4-microorganisms-11-00308" class="html-bibr">4</a>]. Biochar as an amendment to compost was shown to improve aeration and increase composting temperatures and the duration of the thermophilic phase [<a href="#B5-microorganisms-11-00308" class="html-bibr">5</a>,<a href="#B6-microorganisms-11-00308" class="html-bibr">6</a>].</div><div class='html-p'>Pathogens are usually not adapted to the high temperatures during the thermophilic phase of composting, which leads to their inactivation [<a href="#B4-microorganisms-11-00308" class="html-bibr">4</a>,<a href="#B7-microorganisms-11-00308" class="html-bibr">7</a>,<a href="#B8-microorganisms-11-00308" class="html-bibr">8</a>]. One of the concerns with the use of human excreta in agriculture are antibiotic resistances that could be introduced into the food chain and thereby promote the antibiotic crisis. The accumulation of multiple resistances with a lack of treatment options is a public health burden, leading to an estimated minimum of 700,000 deaths per year [<a href="#B9-microorganisms-11-00308" class="html-bibr">9</a>,<a href="#B10-microorganisms-11-00308" class="html-bibr">10</a>]. There are indications that clinically relevant resistances may be transferred back from environmental to clinical bacteria [<a href="#B11-microorganisms-11-00308" class="html-bibr">11</a>]. This highlights the importance of preventing the contamination of the environment with antibiotic resistance genes (ARGs). Thermophilic composting is increasingly analyzed regarding its impact on ARGs. The decrease of genes conferring resistance to different antibiotic classes has been described. Nevertheless, the efficiency varies in different studies, and some ARGs were found to show a tendency to persist or increase [<a href="#B12-microorganisms-11-00308" class="html-bibr">12</a>,<a href="#B13-microorganisms-11-00308" class="html-bibr">13</a>,<a href="#B14-microorganisms-11-00308" class="html-bibr">14</a>,<a href="#B15-microorganisms-11-00308" class="html-bibr">15</a>,<a href="#B16-microorganisms-11-00308" class="html-bibr">16</a>,<a href="#B17-microorganisms-11-00308" class="html-bibr">17</a>,<a href="#B18-microorganisms-11-00308" class="html-bibr">18</a>]. Biochar amendments to compost can augment the decrease in ARG levels during thermophilic composting of manure, but studies are scarce [<a href="#B16-microorganisms-11-00308" class="html-bibr">16</a>,<a href="#B19-microorganisms-11-00308" class="html-bibr">19</a>]. Li and colleagues showed that the majority of ARGs analyzed were significantly less abundant in chicken manure compost containing 5–20% biochar [<a href="#B19-microorganisms-11-00308" class="html-bibr">19</a>]. Cui et al. assessed the impact of different biochars on chicken manure composting. Interestingly, different trends were observed, with mushroom biochar enhancing the removal of ARGs and rice straw biochar decreasing the removal rate, as compared with the control treatment [<a href="#B20-microorganisms-11-00308" class="html-bibr">20</a>].</div><div class='html-p'>Since their discovery, beta-lactam resistances have been shown to rise in Europe [<a href="#B21-microorganisms-11-00308" class="html-bibr">21</a>]. Especially challenging are carbapenemases and extended-spectrum beta-lactamases (ESBL). The first are active against carbapenems, broad-spectrum beta-lactam antibiotics that are usually used for the treatment of infections with multidrug-resistant pathogens. Rising abundances increase the threat of a lack of treatment options [<a href="#B22-microorganisms-11-00308" class="html-bibr">22</a>]. Carbapenemases are found within the Ambler classes A (IMI, SME, NMC, KPC, GES), B (e.g., IMP, VIM, SPM, NDM), and D (e.g., OXA-23, OXA-48) [<a href="#B23-microorganisms-11-00308" class="html-bibr">23</a>]. The IMP-enzymes (imipenem-resistant <span class='html-italic'>Pseudomonas</span>-type carbapenemase) have been known since 1991, when they were first described in <span class='html-italic'>Pseudomonas aeruginosa</span> [<a href="#B24-microorganisms-11-00308" class="html-bibr">24</a>]. The corresponding <span class='html-italic'>bla<sub>IMP</sub></span> genes are usually encoded on class 1 integrons and have spread to several clinically relevant bacteria, such as <span class='html-italic'>Enterobacteriaceae</span> and <span class='html-italic'>Acinetobacter</span> species [<a href="#B22-microorganisms-11-00308" class="html-bibr">22</a>,<a href="#B23-microorganisms-11-00308" class="html-bibr">23</a>,<a href="#B25-microorganisms-11-00308" class="html-bibr">25</a>,<a href="#B26-microorganisms-11-00308" class="html-bibr">26</a>,<a href="#B27-microorganisms-11-00308" class="html-bibr">27</a>]. <span class='html-italic'>Enterobacteriaceae</span>, including carbapenemase-producing ones, have been identified in many environments, including rivers, estuaries and coastal waters [<a href="#B28-microorganisms-11-00308" class="html-bibr">28</a>,<a href="#B29-microorganisms-11-00308" class="html-bibr">29</a>], marine salterns [<a href="#B30-microorganisms-11-00308" class="html-bibr">30</a>], urban topsoils and dust aerosols [<a href="#B31-microorganisms-11-00308" class="html-bibr">31</a>], or edible vegetables [<a href="#B32-microorganisms-11-00308" class="html-bibr">32</a>,<a href="#B33-microorganisms-11-00308" class="html-bibr">33</a>]. <span class='html-italic'>Acinetobacter</span> species are likewise ubiquitous in the environment, such as aqueous and soil habitats, food and animals [<a href="#B34-microorganisms-11-00308" class="html-bibr">34</a>,<a href="#B35-microorganisms-11-00308" class="html-bibr">35</a>].</div><div class='html-p'>ESBLs can degrade penicillins, third-generation cephalosporins and monobactams. Among nosocomial infections, ESBL-producing <span class='html-italic'>Enterobacteriaceae</span> are highly prevalent, and associated with more severe clinical courses of a disease or mortality, as compared with non-ESBL nosocomial infections [<a href="#B36-microorganisms-11-00308" class="html-bibr">36</a>,<a href="#B37-microorganisms-11-00308" class="html-bibr">37</a>,<a href="#B38-microorganisms-11-00308" class="html-bibr">38</a>]. Most ESBLs belong to Ambler class A. However, some OXA-variants (class D) inactivate third-generation cephalosporins, which additionally categorizes them as ESBLs. TEM-1 (Temoneira) was the first described beta-lactamase encoded on a plasmid [<a href="#B23-microorganisms-11-00308" class="html-bibr">23</a>,<a href="#B39-microorganisms-11-00308" class="html-bibr">39</a>,<a href="#B40-microorganisms-11-00308" class="html-bibr">40</a>]; whereas TEM-1 is a penicillinase, most of the 183 TEM variants described so far belong to ESBLs. TEM is highly prevalent, and associated with clinical <span class='html-italic'>Enterobacteriaceae</span>, <span class='html-italic'>Neisseria gonorrhoeae</span> and to a lesser extent <span class='html-italic'>P.s aeruginosa</span> [<a href="#B23-microorganisms-11-00308" class="html-bibr">23</a>,<a href="#B40-microorganisms-11-00308" class="html-bibr">40</a>]. First identified in the 1980s, CTX-M (cefotaximase from Munich) belongs to the most prevalent ESBLs nowadays, and is even more prevalent than TEM and SHV-5. The <span class='html-italic'>bla<sub>CTX-M</sub></span> genes are associated with plasmids, transposons and insertion sequences and are found in many clinically relevant bacteria, e.g., <span class='html-italic'>Enterobacteriaceae</span>, <span class='html-italic'>Salmonella</span>, <span class='html-italic'>Shigella</span> or <span class='html-italic'>Serratia</span> [<a href="#B23-microorganisms-11-00308" class="html-bibr">23</a>,<a href="#B40-microorganisms-11-00308" class="html-bibr">40</a>,<a href="#B41-microorganisms-11-00308" class="html-bibr">41</a>].</div><div class='html-p'>Integrons contribute to the horizontal gene transfer (HGT) of ARGs, since they often acquire ARG-encoding gene cassettes that can be mobilized by the encoded integrase at the recombination sites (<span class='html-italic'>attC</span>). Excised gene cassettes may be inserted into other integrons at their recombination sites (<span class='html-italic'>attI</span>). Whereas chromosomal integrons sometimes carry hundreds of gene cassettes of diverse or unknown functions, mobile and clinically relevant class 1 integrons usually harbor a maximum of six gene cassettes, mostly encoding ARGs [<a href="#B42-microorganisms-11-00308" class="html-bibr">42</a>]. The insertion and excision of genes are mediated by a tyrosine recombinase, the integron-integrase encoded by the <span class='html-italic'>intI</span> gene. Class 1 integron-integrases are primarily associated with clinical bacteria [<a href="#B42-microorganisms-11-00308" class="html-bibr">42</a>]. The respective <span class='html-italic'>intI1</span> gene is therefore used as a marker gene for anthropogenic pollution [<a href="#B42-microorganisms-11-00308" class="html-bibr">42</a>,<a href="#B43-microorganisms-11-00308" class="html-bibr">43</a>,<a href="#B44-microorganisms-11-00308" class="html-bibr">44</a>].</div><div class='html-p'>Plasmids of the IncP-1 group have a broad host range and are self-transmissible [<a href="#B45-microorganisms-11-00308" class="html-bibr">45</a>,<a href="#B46-microorganisms-11-00308" class="html-bibr">46</a>]. They have been found in diverse environments such as soil [<a href="#B47-microorganisms-11-00308" class="html-bibr">47</a>,<a href="#B48-microorganisms-11-00308" class="html-bibr">48</a>,<a href="#B49-microorganisms-11-00308" class="html-bibr">49</a>], the rhizosphere [<a href="#B49-microorganisms-11-00308" class="html-bibr">49</a>,<a href="#B50-microorganisms-11-00308" class="html-bibr">50</a>], manure [<a href="#B51-microorganisms-11-00308" class="html-bibr">51</a>], wastewater [<a href="#B52-microorganisms-11-00308" class="html-bibr">52</a>], rivers [<a href="#B53-microorganisms-11-00308" class="html-bibr">53</a>] and estuarine water [<a href="#B54-microorganisms-11-00308" class="html-bibr">54</a>]. IncP-1 plasmids are associated with antibiotic and metal resistance, as well as catabolic functional genes. Being highly efficient at HGT, they are considered important vectors for the dissemination of ARGs [<a href="#B45-microorganisms-11-00308" class="html-bibr">45</a>,<a href="#B55-microorganisms-11-00308" class="html-bibr">55</a>,<a href="#B56-microorganisms-11-00308" class="html-bibr">56</a>]. KorB is involved in global regulation and partitioning of IncP-1 plasmids. The corresponding <span class='html-italic'>korB</span> gene is highly conserved, and therefore serves as a marker gene for IncP-1 plasmids and HGT [<a href="#B57-microorganisms-11-00308" class="html-bibr">57</a>,<a href="#B58-microorganisms-11-00308" class="html-bibr">58</a>].</div><div class='html-p'>We have assessed the fate of the highly prevalent and environmentally important carbapenemase gene <span class='html-italic'>bla<sub>IMP</sub></span> [<a href="#B59-microorganisms-11-00308" class="html-bibr">59</a>,<a href="#B60-microorganisms-11-00308" class="html-bibr">60</a>], the ESBL genes <span class='html-italic'>bla<sub>CTX-M</sub></span> and <span class='html-italic'>bla<sub>TEM</sub></span>, as well as the class 1 integron-integrase gene <span class='html-italic'>intI1</span> and IncP-1 plasmid regulator gene <span class='html-italic'>korB</span> as indicators of HGT during the thermophilic composting of human excreta from dry toilets amended with green cuttings, straw, and with and without biochar. Gene-specific TaqMan qPCR was applied to samples taken before, after 14 days, and at the end of composting, after 5 and 5.5 months.</div><div class='html-p'>We previously assessed the bacterial community in the same samples using 16S rRNA gene amplicon sequencing, as well as cultivation-based approaches, to analyze potential human pathogens in the mature compost [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>]. Moreover, we performed metagenomic sequencing and qPCR, analyzing virulence factors and ARGs conferring resistance to different antibiotic classes [<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>]. This study complements our previous publications by examining the fate of environmentally relevant beta-lactamase genes and the impact of thermophilic composting on the horizontal spread of resistance genes.</div></section><section id='sec2-microorganisms-11-00308' type=''><h2 data-nested='1'> 2. Materials and Methods</h2><section id='sec2dot1-microorganisms-11-00308' type=''><h4 class='html-italic' data-nested='2'> 2.1. Composting Trial and Sampling</h4><div class='html-p'>Experimental set-up of the thermophilic composting trial, as well as physico-chemical parameters of the composting substrates were described in detail in [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>]. In brief, dry toilet contents, along with green cuttings, straw, and urine, with or without biochar, were composted in windrows in Eckernförde, Germany, between September 2018 and January 2019. The two treatments with (“-B”) and without biochar were set up twice, at an interval of 2 weeks. E1 and E1-B indicate the first, and E2 and E2-B the second repetition of the trial.</div><div class='html-p'>Samples were taken on the day of experimental set-up (“start”), after two weeks (“14 days”, only repetition 1), and at the end of the trials after 5.5 (repetition 1) and 5 months (repetition 2), respectively, to compare the initial load of ARGs with the finished product intended to be used in agriculture. The samples after 14 days of composting represent the thermophilic phase [<a href="#B63-microorganisms-11-00308" class="html-bibr">63</a>]. Unfortunately, samples after 14 days of composting were only available from repetition 1.</div></section><section id='sec2dot2-microorganisms-11-00308' type=''><h4 class='html-italic' data-nested='2'> 2.2. DNA Extraction and Purification</h4><div class='html-p'>Total DNA was extracted using the NucleoSpin<sup>®</sup> Soil gDNA extraction kit (Macherey-Nagel), according to the manufacturer’s instructions. From each sample, 250 mg compost were used, along with lysis buffer SL2 without enhancer. Mechanical disruption was conducted in a FastPrep-24<sup>™</sup> 5G homogenizer (MP Biomedicals) at 5 m/s for 30 s. A total of 50 µL nuclease-free water was used for DNA elution. The DNA extracts were further purified using a magnetic separation device (NucleoMag<sup>®</sup> SEP, Macherey-Nagel) and the CleanNGS kit (CleanNA, according to the manufacturer’s instructions. Preliminary tests revealed an optimal dilution of 1:100 for qPCR.</div></section><section id='sec2dot3-microorganisms-11-00308' type=''><h4 class='html-italic' data-nested='2'> 2.3. Quantitative RealTime PCR</h4><div class='html-p'>The qPCR analyses were conducted as stated in [<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>] on a LightCycler<sup>®</sup> 480 II instrument (Roche Diagnostics Ltd.). Primer sequences, PCR amplicon sizes, and references are listed in <a href="#microorganisms-11-00308-t001" class="html-table">Table 1</a>. The composition of the qPCR mastermix for all <span class='html-italic'>bla</span> genes and <span class='html-italic'>intI1</span> was as follows: 1x LightCycler<sup>®</sup> Probes Master (Roche Diagnostics Germany GmbH), 0.5 µM each primer, 0.1 µM probe, 5 µL template (1:100 dilution of DNA extracts). The total volume per reaction was 20 µL. PCR programs were initiated with a denaturation step at 95 °C for 10 min, followed by 45 cycles of denaturation at 95 °C for 10 s, and annealing/elongation at 60 °C (<span class='html-italic'>bla<sub>CTX-M</sub></span>, <span class='html-italic'>bla<sub>IMP</sub></span>, <span class='html-italic'>intI1</span>) or 56 °C (<span class='html-italic'>bla<sub>TEM</sub></span>), and 72 °C for 1 s. Standard curves of 10<sup>1</sup> to 10<sup>8</sup> copies of purified PCR products were used for quantification. The <span class='html-italic'>korB</span> qPCR mastermix consisted of 1x LightCycler<sup>®</sup> Probes Master, 0.4 µM primers -F and -R, 0.2 µM primers -Fz, -Rge, and -Rd, 0.15 µM each probe, 5 µL template (1:100 dilution of DNA extracts), H<sub>2</sub>O ad. 20 µL. The qPCR program was initiated at 95 °C for 5 min, followed by 40 cycles of 95 °C for 15 s; 54 °C for 15 s; and 60 °C, 1 min, including fluorescence measurement. An equimolar mix of plasmids representing different IncP-1 groups was used as a standard for quantification (<a href="#microorganisms-11-00308-t001" class="html-table">Table 1</a>). Gene copy-numbers were normalized to the 16S rRNA gene and to 1 g compost. Two-tailed, paired Student’s t-test was conducted to define significant differences between start and end samples of composting. Due to the small number of replicates in the start samples (one mixed sample per compost pile), treatments were combined to test for significance. This means that four values from the start of composting were tested against four values from the end (the mean of the triplicates per pile, respectively). Additionally, the biological replicates of the end samples were analyzed for differences between the two treatments with and without biochar, which means six samples per treatment. This was conducted for both normalizations (to 1 g compost and to the 16S rRNA gene copies). The <span class='html-italic'>p</span>-values below 0.05 indicate statistical significance, and <span class='html-italic'>p</span>-values below 0.01 are considered highly significant.</div></section></section><section id='sec3-microorganisms-11-00308' type='results'><h2 data-nested='1'> 3. Results</h2><div class='html-p'>The present study aimed to analyze the fate of beta-lactamase genes, as well as marker genes for HGT during thermophilic composting of human excreta. These results complement a previous study on the same samples analyzing antibiotic resistance and virulence genes [<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>].</div><div class='html-p'><a href="#microorganisms-11-00308-f001" class="html-fig">Figure 1</a> shows the qPCR results during composting for the ARGs <span class='html-italic'>bla<sub>IMP</sub></span>, <span class='html-italic'>bla<sub>CTX-M</sub></span> and <span class='html-italic'>bla<sub>TEM</sub></span>. No significant changes between start and end samples were observed. For <span class='html-italic'>bla<sub>IMP</sub></span>, copy numbers in the final samples show higher variance than copy numbers in the initial samples. <span class='html-italic'>Bla<sub>CTX-M</sub></span> and <span class='html-italic'>bla<sub>TEM</sub></span> copy numbers exhibit the opposite trend, with higher variances in the start samples (data not shown). The qPCR data for <span class='html-italic'>intI1</span> and <span class='html-italic'>korB</span> as marker genes for HGT are shown in <a href="#microorganisms-11-00308-f002" class="html-fig">Figure 2</a>. Both genes show significant decreases during thermophilic composting. No significant differences were detected between the treatments with biochar (E1-B, E2-B) and without biochar (E1, E2) in the mature compost for any of the genes analyzed (data not shown).</div><div class='html-p'><a href="#microorganisms-11-00308-t002" class="html-table">Table 2</a> depicts the percentage decrease or increase in gene copy numbers during composting. The beta-lactamase genes exhibited mean increases of gene copy numbers from start to end of composting, when changes in % of all treatments were averaged. Only <span class='html-italic'>bla<sub>TEM</sub></span> normalized to 1 g compost showed a mean decrease of 42.6%. <span class='html-italic'>IntI1</span> copy numbers decreased significantly by 90% (normalized to 16S rRNA gene copies) or 95.8% (normalized to 1 g compost) from start to end of composting. <span class='html-italic'>KorB</span> copy numbers exhibited significant decreases of 99.0% (normalized to 16S rRNA gene copies) and 99.5% (normalized to 1 g compost), respectively.</div><div class='html-p'><a href="#microorganisms-11-00308-t003" class="html-table">Table 3</a> depicts mean gene copy numbers of start and end of composting, irrespective of the treatments with or without biochar. This partly shows different trends, as compared with the calculated decrease or increase shown in <a href="#microorganisms-11-00308-t002" class="html-table">Table 2</a>. Mean gene copy numbers of <span class='html-italic'>bla<sub>IMP</sub></span> increased during composting, whereas <span class='html-italic'>bla<sub>CTX-M</sub></span> decreased slightly and <span class='html-italic'>bla<sub>TEM</sub></span> decreased by two orders of magnitude. <span class='html-italic'>IntI1</span> and <span class='html-italic'>korB</span> decreased by one and three orders of magnitude, respectively (<a href="#microorganisms-11-00308-t003" class="html-table">Table 3</a>). The abundances of <span class='html-italic'>intI1</span> and <span class='html-italic'>korB</span> genes were significantly different in both the start and end samples of composting.</div></section><section id='sec4-microorganisms-11-00308' type='discussion'><h2 data-nested='1'> 4. Discussion</h2><div class='html-p'>The study aimed to assess the fate of three beta-lactam ARGs of high clinical relevance, as well as the class 1 integron-integrase gene <span class='html-italic'>intI1</span> and <span class='html-italic'>korB</span> marker gene for IncP-1 plasmids as indicators for HGT during thermophilic composting of human excreta amended with green cuttings and straw, with and without biochar. The trial and results of cultivation-based and molecular analyses on the bacterial community and ARGs for different antibiotic classes have been published previously [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>,<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>].</div><div class='html-p'><span class='html-italic'>Bla<sub>IMP</sub></span>, <span class='html-italic'>bla<sub>CTX-M</sub></span> and <span class='html-italic'>bla<sub>TEM</sub></span> were chosen for the study, since these three genes exhibited increases from start to end of composting in shotgun metagenomic sequencing data on the same samples (data not shown). Using the more sensitive qPCR method, we aimed to quantify this trend. However, the qPCR results showed no clear pattern of changes during composting, with inconsistency in abundances among the replicate samples. This might be due to large variations in the copy numbers of the replicates from the end of composting. Since a sample dilution of 1:100 was applied to avoid inhibition of the qPCR, gene copy numbers in the reactions were very low, in both initial and final samples. This leads to higher variation in replicate measurements [<a href="#B68-microorganisms-11-00308" class="html-bibr">68</a>]. The <span class='html-italic'>bla</span> gene abundances were also low in comparison with other ARGs analyzed before [<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>]. This is interesting, since metagenomic data on the same samples revealed the highest abundance for the group of beta-lactam ARGs, with significant decreases during composting [<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>]. However, the high abundance in the metagenomic data might be due to the broad variety of genes in this group of ARGs, rather than a high prevalence of individual genes [<a href="#B69-microorganisms-11-00308" class="html-bibr">69</a>]. In line with low abundances of the beta-lactamase genes analyzed here, we have not detected <span class='html-italic'>bla<sub>IMP</sub></span> or <span class='html-italic'>bla<sub>CTX-M</sub></span> through conventional PCR, either [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>].</div><div class='html-p'>Although IMP-type carbapenemases are distributed worldwide and are endemic in some countries [<a href="#B25-microorganisms-11-00308" class="html-bibr">25</a>,<a href="#B26-microorganisms-11-00308" class="html-bibr">26</a>], their genes have hardly been screened in environmental studies [<a href="#B70-microorganisms-11-00308" class="html-bibr">70</a>,<a href="#B71-microorganisms-11-00308" class="html-bibr">71</a>]. To the best of our knowledge, there are no composting studies that have examined the fate of <span class='html-italic'>bla<sub>IMP</sub></span>. A study of the antibiotic resistance profiles of <span class='html-italic'>P. aeruginosa</span> isolates from different composts and their feedstock materials highlighted the prevalence of carbapenemases. A total of 24% of the isolates showed resistance to carbapenems. Thus, it was one of the most prevalent resistances among the isolates [<a href="#B72-microorganisms-11-00308" class="html-bibr">72</a>]. Carbapenems are used to treat <span class='html-italic'>P. aeruginosa</span> infections and are therefore often associated with this pathogen [<a href="#B73-microorganisms-11-00308" class="html-bibr">73</a>,<a href="#B74-microorganisms-11-00308" class="html-bibr">74</a>]. Although culture conditions were selective for <span class='html-italic'>P. aeruginosa</span>, we could only affiliate four isolates out of two hundred from the mature compost to <span class='html-italic'>P. aeruginosa</span> [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>]. Their low prevalence might be one reason for the overall low <span class='html-italic'>bla<sub>IMP</sub></span> abundances. The <span class='html-italic'>bla<sub>IMP</sub></span> gene is also associated with carbapenem-resistant <span class='html-italic'>Enterobacteriaceae</span> [<a href="#B25-microorganisms-11-00308" class="html-bibr">25</a>,<a href="#B26-microorganisms-11-00308" class="html-bibr">26</a>]. <span class='html-italic'>Enterobacteriaceae</span> sequences were found in a 16S rRNA amplicon sequence analysis conducted previously on the same samples with abundances of between 0 and 1.5% of the total bacterial sequences [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>]. Shotgun metagenomic sequencing data showed a broader variety of <span class='html-italic'>Enterobacteriaceae</span> with slightly decreasing relative abundances during composting, from 0.3–0.4% to 0.26–0.27% relative abundances (data not shown). In a recent study by Willms and colleagues, <span class='html-italic'>bla<sub>IMP</sub></span> genes were found in grassland and forest soils. Abundances did not differ among different land use strategies. However, a positive correlation with fungal diversity was detected [<a href="#B47-microorganisms-11-00308" class="html-bibr">47</a>]. This might explain rising <span class='html-italic'>bla<sub>IMP</sub></span> abundances in the compost, since fungi contribute to composting rise in abundance during the composting process [<a href="#B4-microorganisms-11-00308" class="html-bibr">4</a>].</div><div class='html-p'><span class='html-italic'>Bla<sub>CTX-M</sub></span> showed lowest abundances of 3.6 × 10<sup>3</sup> copies/g out of 18 ARGs analyzed in pig manure composting [<a href="#B75-microorganisms-11-00308" class="html-bibr">75</a>]. The copy numbers were around one order of magnitude lower than in the present trial. A comparatively low abundance of different <span class='html-italic'>bla<sub>CTX-M</sub></span> genes seems to be typical for thermophilic composting of animal manures (cattle, cow, chicken, sheep) [<a href="#B18-microorganisms-11-00308" class="html-bibr">18</a>,<a href="#B76-microorganisms-11-00308" class="html-bibr">76</a>,<a href="#B77-microorganisms-11-00308" class="html-bibr">77</a>]. Most studies found a decline in the gene during composting [<a href="#B18-microorganisms-11-00308" class="html-bibr">18</a>,<a href="#B75-microorganisms-11-00308" class="html-bibr">75</a>,<a href="#B76-microorganisms-11-00308" class="html-bibr">76</a>,<a href="#B78-microorganisms-11-00308" class="html-bibr">78</a>]. In sewage sludge and mushroom composting, <span class='html-italic'>bla<sub>CTX-M</sub></span> abundance also decreased [<a href="#B79-microorganisms-11-00308" class="html-bibr">79</a>]. Our results do not draw a clear picture, since abundances during the composting process vary for the different treatments and the two composting trials.</div><div class='html-p'><span class='html-italic'>Bla<sub>TEM</sub></span> is prevalent in European wastewater-treatment plants [<a href="#B80-microorganisms-11-00308" class="html-bibr">80</a>,<a href="#B81-microorganisms-11-00308" class="html-bibr">81</a>]. Even in the feces of healthy persons without antibiotic treatment, <span class='html-italic'>bla<sub>TEM</sub></span> is frequently detected [<a href="#B82-microorganisms-11-00308" class="html-bibr">82</a>,<a href="#B83-microorganisms-11-00308" class="html-bibr">83</a>]. Zhang et al. reported decreases in <span class='html-italic'>bla<sub>TEM</sub></span> during sewage sludge composting [<a href="#B79-microorganisms-11-00308" class="html-bibr">79</a>]. Interestingly, the sample peaking in gene abundance (E1-B start) corresponds to the metagenomic sequencing data from the same samples. E1 and E2 exhibited increases during composting, whereas no <span class='html-italic'>bla<sub>TEM</sub></span> sequences were detected in E2-B in the metagenomic data (data not shown).</div><div class='html-p'>Significant decreases in <span class='html-italic'>intI1</span> indicate that the composting process reshaped the bacterial community with respect to decreasing the abundance of clinical strains. This corresponds with amplicon and metagenomic sequencing results from the same samples, where decreases in potential human pathogens could be shown [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>,<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>]. In a clinical context, class 1 integron-integrase genes are most common, whereas a broader variety of integron classes is found in the environment [<a href="#B42-microorganisms-11-00308" class="html-bibr">42</a>]. The <span class='html-italic'>intI1</span> gene serves as a proxy for ARGs, since class 1 integrons frequently carry resistance genes [<a href="#B43-microorganisms-11-00308" class="html-bibr">43</a>]. The significant decline in <span class='html-italic'>intI1</span> in this study is in line with our previous analyses. Metagenomics, as well as PCR and qPCR results showed decreases in ARGs conferring resistance to different classes of antibiotics [<a href="#B61-microorganisms-11-00308" class="html-bibr">61</a>,<a href="#B62-microorganisms-11-00308" class="html-bibr">62</a>]. Decreases in <span class='html-italic'>intI1</span> in a study on sewage sludge composting with and without biochar and hyperthermophiles were in the same range as in this work [<a href="#B84-microorganisms-11-00308" class="html-bibr">84</a>]. Liao et al. showed the positive effect of high temperatures on <span class='html-italic'>intI1</span> removal rates, by comparing hyperthermophilic and thermophilic composting of sewage sludge [<a href="#B85-microorganisms-11-00308" class="html-bibr">85</a>].</div><div class='html-p'>The addition of 5 mass-% biochar to sewage sludge compost resulted in <span class='html-italic'>intI1</span> copy numbers of 4 × 10<sup>5</sup>, as compared with 6 × 10<sup>7</sup> in the control treatment without biochar [<a href="#B86-microorganisms-11-00308" class="html-bibr">86</a>]. The addition of 2 or 5% biochar increased the removal efficiency of <span class='html-italic'>intI1</span> gene copies in sewage sludge composting with hyperthermophiles [<a href="#B84-microorganisms-11-00308" class="html-bibr">84</a>]. In the present study, biochar addition of 3–4% did not result in different gene abundances after composting.</div><div class='html-p'>Significant decreases in <span class='html-italic'>korB</span> gene abundances by 99 and 99.5% demonstrated that the composting process can contribute markedly to the decline of IncP-1 plasmids. This group of plasmids is strongly associated with ARGs and HGT [<a href="#B45-microorganisms-11-00308" class="html-bibr">45</a>,<a href="#B55-microorganisms-11-00308" class="html-bibr">55</a>,<a href="#B56-microorganisms-11-00308" class="html-bibr">56</a>]. Their decrease therefore indicates a reduced potential for the dissemination of ARGs in the mature compost. To the best of our knowledge, there are only very few studies investigating IncP-1 plasmids in compost or compost-amended soil. Wu and colleagues demonstrated higher removal of IncP plasmids marker gene copies with higher temperatures during pig manure composting [<a href="#B87-microorganisms-11-00308" class="html-bibr">87</a>]. IncP-1 plasmids have been detected in various environments, often associated with xenobiotic pollution [<a href="#B88-microorganisms-11-00308" class="html-bibr">88</a>]. The recently discovered high diversity of IncP-1 plasmids in the rhizosphere points to the important ecological role of these plasmids [<a href="#B50-microorganisms-11-00308" class="html-bibr">50</a>].</div><div class='html-p'>The occurrence of <span class='html-italic'>intI1</span> gene copies was significantly higher than that of <span class='html-italic'>korB</span> gene copies in both initial and final samples of composting. This indicates that many of the class 1 integrons are not associated with IncP-1 plasmids. Interestingly, the relative abundance of both genes was previously shown to be correlated with each other in soils subject to mineral fertilization. However, contrary to our data, the concentration of <span class='html-italic'>korB</span> was higher than <span class='html-italic'>intI1</span> in the rhizosphere samples [<a href="#B49-microorganisms-11-00308" class="html-bibr">49</a>]. Both <span class='html-italic'>intI1</span> and <span class='html-italic'>korB</span> serve as signature genes for the transferability of ARGs via HGT. Our <span class='html-italic'>korB</span> data show a significant decrease in IncP-1 plasmids during thermophilic composting. Thus, we provide important information on the decreasing potential of HGT due to thermophilic composting.</div><div class='html-p'>In conclusion, the beta-lactamase genes <span class='html-italic'>bla<sub>IMP</sub></span>, <span class='html-italic'>bla<sub>CTX-M</sub></span>, and <span class='html-italic'>bla<sub>TEM</sub></span> were detected before and after thermophilic composting of human excreta, in low concentrations. Clear trends in the decrease or increase of copy numbers were not observed. Significant decreases in <span class='html-italic'>intI1</span> and <span class='html-italic'>korB</span> genes indicate a decline in clinically relevant bacteria and HGT during composting. This suggests that thermophilic composting can be effective in reducing the public health burden of human excreta.</div></section> </div> <div class="html-back"> <section class='html-notes'><h2 >Author Contributions</h2><div class='html-p'>Conceptualization, K.A.W., K.P., N.B. and E.G.; methodology, K.A.W. and E.G.; validation, K.A.W. and E.G.; formal analysis, K.A.W.; investigation, K.A.W. and L.F.; resources, E.G.; writing—original draft preparation, K.A.W.; writing—review and editing, L.F., T.H., N.B., K.P. and E.G.; visualization, K.A.W.; supervision, N.B., K.P. and E.G.; project administration, N.B., K.P. and E.G.; funding acquisition, N.B., K.P. and E.G. All authors have read and agreed to the published version of the manuscript.</div></section><section class='html-notes'><h2>Funding</h2><div class='html-p'>This research was funded by the BMBF (Federal Ministry of Education and Research), grant number 01DG18005 to E.G., being part of the ClimEtSan-Project, funded by BMBF grant number 01DG17010 and the DAAD (German Academic Exchange Service), grant number 57354010.</div></section><section class='html-notes'><h2 >Institutional Review Board Statement</h2><div class='html-p'>Not applicable.</div></section><section class='html-notes'><h2 >Informed Consent Statement</h2><div class='html-p'>Not applicable.</div></section><section class='html-notes'><h2 >Data Availability Statement</h2><div class='html-p'>Not applicable.</div></section><section id='html-ack' class='html-ack'><h2 >Acknowledgments</h2><div class='html-p'>We want to express our gratitude to Finizio GmbH and Goldeimer gGmbH, especially Florian Augustin and Enno Schröder, for the opportunity to join the composting trial, as well as their helpfulness and dedication throughout the project. We gratefully acknowledge the possibility of conducting the composting trials at the “Recyclinghof Eckernförde”. Thanks to Kornelia Smalla and Sara Gallego (Julius-Kühn-Institute Braunschweig), Laurent Poirel (University of Fribourg), as well as to Yvonne Pfeiffer and Guido Werner (Robert Koch Institute) for providing reference strains for qPCR. K.A.W. thanks the “Agriculture and Environment Foundation” and “Berliner Hochschule für Technik” (BHT) for the Ph.D. fellowships.</div></section><section class='html-notes'><h2 >Conflicts of Interest</h2><div class='html-p'>The authors declare no conflict of interest.</div></section><section id='html-references_list'><h2>References</h2><ol class='html-xx'><li id='B1-microorganisms-11-00308' class='html-x' data-content='1.'>Rose, C.; Parker, A.; Jefferson, B.; Cartmell, E. The characterization of feces and urine: A review of the literature to inform advanced treatment technology. <span class='html-italic'>Crit. Rev. Environ. Sci. 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Impacts of pile temperature on antibiotic resistance, metal resistance and microbial community during swine manure composting. <span class='html-italic'>Sci. Total Environ.</span> <b>2020</b>, <span class='html-italic'>744</span>, 140920. 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[<a href="https://scholar.google.com/scholar_lookup?title=Cultivation-Independent+Screening+Revealed+Hot+Spots+of+IncP-1,+IncP-7+and+IncP-9+Plasmid+Occurrence+in+Different+Environmental+Habitats&author=Dealtry,+S.&author=Ding,+G.-C.&author=Weichelt,+V.&author=Dunon,+V.&author=Schl%C3%BCter,+A.&author=Martini,+M.C.&author=De+Papa,+M.F.&author=Lagares+,+A.&author=Amos,+G.C.A.&author=Wellington,+E.M.H.&publication_year=2014&journal=PLoS+ONE&volume=9&pages=e89922&doi=10.1371/journal.pone.0089922&pmid=24587126" class='google-scholar' target='_blank' rel='noopener noreferrer'>Google Scholar</a>] [<a href="https://doi.org/10.1371/journal.pone.0089922" class='cross-ref' target='_blank' rel='noopener noreferrer'>CrossRef</a>] [<a href="http://www.ncbi.nlm.nih.gov/pubmed/24587126" class='cross-ref' data-typ='pmid' target='_blank' rel='noopener noreferrer'>PubMed</a>]</li></ol></section><section id='FiguresandTables' type='display-objects'><div class="html-fig-wrap" id="microorganisms-11-00308-f001"> <div class='html-fig_img'> <div class="html-figpopup html-figpopup-link" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#fig_body_display_microorganisms-11-00308-f001"> <img alt="Microorganisms 11 00308 g001 550" data-large="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001.png" data-original="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001.png" data-lsrc="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001-550.jpg" /> <a class="html-expand html-figpopup" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#fig_body_display_microorganisms-11-00308-f001"></a> </div> </div> <div class="html-fig_description"> <b>Figure 1.</b> Copy numbers of beta-lactam ARGs <span class='html-italic'>bla<sub>IMP</sub></span> (<b>A</b>,<b>B</b>) and <span class='html-italic'>bla<sub>CTX-M</sub></span> (<b>C</b>,<b>D</b>), as well as <span class='html-italic'>bla<sub>TEM</sub></span> (<b>E</b>,<b>F</b>) as assessed by qPCR. Copy numbers were normalized to 16S rRNA gene copy numbers (left charts) and to 1 g compost (right charts). Bars represent the average of three individual qPCR measurements with standard deviation. Start samples, 14-days samples (only repetition 1) and end samples are depicted individually for the first (E1, E1-B) and second repetition of composting (E2, E2-B). End samples comprise replicates from front (end 1), middle (end 2) and back (end 3) of each compost pile. The <span class='html-italic'>p</span>-values were calculated to compare start with end samples of composting for all four compost piles combined (average of the triplicate end samples, no differentiation of the treatments). None of the sample sets showed significance (<span class='html-italic'>p</span> < 0.05). Therefore, they are indicated by “n.s.” (not significant).  </div> </div> <div class="html-fig_show mfp-hide" id ="fig_body_display_microorganisms-11-00308-f001" > <div class="html-caption" > <b>Figure 1.</b> Copy numbers of beta-lactam ARGs <span class='html-italic'>bla<sub>IMP</sub></span> (<b>A</b>,<b>B</b>) and <span class='html-italic'>bla<sub>CTX-M</sub></span> (<b>C</b>,<b>D</b>), as well as <span class='html-italic'>bla<sub>TEM</sub></span> (<b>E</b>,<b>F</b>) as assessed by qPCR. Copy numbers were normalized to 16S rRNA gene copy numbers (left charts) and to 1 g compost (right charts). Bars represent the average of three individual qPCR measurements with standard deviation. Start samples, 14-days samples (only repetition 1) and end samples are depicted individually for the first (E1, E1-B) and second repetition of composting (E2, E2-B). End samples comprise replicates from front (end 1), middle (end 2) and back (end 3) of each compost pile. The <span class='html-italic'>p</span>-values were calculated to compare start with end samples of composting for all four compost piles combined (average of the triplicate end samples, no differentiation of the treatments). None of the sample sets showed significance (<span class='html-italic'>p</span> < 0.05). Therefore, they are indicated by “n.s.” (not significant).</div> <div class="html-img"><img alt="Microorganisms 11 00308 g001" data-large="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001.png" data-original="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001.png" data-lsrc="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g001.png" /></div> </div><div class="html-fig-wrap" id="microorganisms-11-00308-f002"> <div class='html-fig_img'> <div class="html-figpopup html-figpopup-link" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#fig_body_display_microorganisms-11-00308-f002"> <img alt="Microorganisms 11 00308 g002 550" data-large="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002.png" data-original="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002.png" data-lsrc="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002-550.jpg" /> <a class="html-expand html-figpopup" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#fig_body_display_microorganisms-11-00308-f002"></a> </div> </div> <div class="html-fig_description"> <b>Figure 2.</b> Copy numbers of the class 1 integron-integrase gene <span class='html-italic'>intI1</span> (<b>A</b>,<b>B</b>) and IncP-1 marker gene <span class='html-italic'>korB</span> (<b>C</b>,<b>D</b>) as assessed by qPCR. Copy numbers were normalized to 16S rRNA gene copy numbers (left charts) and to 1 g compost (right charts). Bars represent the average of three individual qPCR measurements with the standard deviation. Start samples, 14-days samples (only repetition 1) and end samples are depicted individually for the first (E1, E1-B) and second repetition of composting (E2, E2-B). End samples comprise replicates from the front (end 1), middle (end 2) and back (end 3) of each compost pile. Asterisks indicate the <span class='html-italic'>p</span>-values obtained from Student’s <span class='html-italic'>t</span>-test (* <span class='html-italic'>p</span> < 0.05, ** <span class='html-italic'>p</span> < 0.01) representing the statistical significance of the data. The <span class='html-italic'>p</span>-values were calculated to compare start with end samples of composting for all four compost piles combined (average of the triplicate end samples, no differentiation of the treatments).  </div> </div> <div class="html-fig_show mfp-hide" id ="fig_body_display_microorganisms-11-00308-f002" > <div class="html-caption" > <b>Figure 2.</b> Copy numbers of the class 1 integron-integrase gene <span class='html-italic'>intI1</span> (<b>A</b>,<b>B</b>) and IncP-1 marker gene <span class='html-italic'>korB</span> (<b>C</b>,<b>D</b>) as assessed by qPCR. Copy numbers were normalized to 16S rRNA gene copy numbers (left charts) and to 1 g compost (right charts). Bars represent the average of three individual qPCR measurements with the standard deviation. Start samples, 14-days samples (only repetition 1) and end samples are depicted individually for the first (E1, E1-B) and second repetition of composting (E2, E2-B). End samples comprise replicates from the front (end 1), middle (end 2) and back (end 3) of each compost pile. Asterisks indicate the <span class='html-italic'>p</span>-values obtained from Student’s <span class='html-italic'>t</span>-test (* <span class='html-italic'>p</span> < 0.05, ** <span class='html-italic'>p</span> < 0.01) representing the statistical significance of the data. The <span class='html-italic'>p</span>-values were calculated to compare start with end samples of composting for all four compost piles combined (average of the triplicate end samples, no differentiation of the treatments).</div> <div class="html-img"><img alt="Microorganisms 11 00308 g002" data-large="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002.png" data-original="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002.png" data-lsrc="/microorganisms/microorganisms-11-00308/article_deploy/html/images/microorganisms-11-00308-g002.png" /></div> </div><div class="html-table-wrap" id="microorganisms-11-00308-t001"> <div class="html-table_wrap_td" > <div class="html-tablepopup html-tablepopup-link" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href='#table_body_display_microorganisms-11-00308-t001'> <img alt="Table" data-lsrc="https://www.mdpi.com/img/table.png" /> <a class="html-expand html-tablepopup" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#table_body_display_microorganisms-11-00308-t001"></a> </div> </div> <div class="html-table_wrap_discription"> <b>Table 1.</b> qPCR primers and probes with target gene, oligonucleotide primer sequence, amplicon size, positive control for generation of standards, and primer/probe reference. Standard curves of 10<sup>1</sup> to 10<sup>8</sup> gene copies were used for quantification. </div> </div> <div class="html-table_show mfp-hide " id ="table_body_display_microorganisms-11-00308-t001" > <div class="html-caption" ><b>Table 1.</b> qPCR primers and probes with target gene, oligonucleotide primer sequence, amplicon size, positive control for generation of standards, and primer/probe reference. Standard curves of 10<sup>1</sup> to 10<sup>8</sup> gene copies were used for quantification.</div> <table > <thead ><tr ><th align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Target Gene</th><th align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Primer/<br>Probe</th><th align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Sequence (5′→3′)</th><th align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Amplicon Size [bp]</th><th align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Positive Controls</th><th align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Reference</th></tr></thead><tbody ><tr ><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>CTX-M</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_CTXM-F</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >CAGCTGGGAGACGAAACGTT</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >63</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>E. coli</span> 1058_16 *</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >[<a href="#B64-microorganisms-11-00308" class="html-bibr">64</a>]</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_CTXM-R</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >CCGGAATGGCGGTGTTTA</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_CTXM-P</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >6FAM-<br>CGTCTCGACCGTACCGAGCCGAC-TAMRA</td></tr><tr ><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>IMP</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_blaIMP-F</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >ATTTTCATAGTGACAGCACGGGC</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >105</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>Klebsiella pneumoniae</span> R77 IMP-4</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >[<a href="#B65-microorganisms-11-00308" class="html-bibr">65</a>]</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_blaIMP-R</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >CCTTACCGTCTTTTTTAAGCAGCTCATTAG</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_blaIMP-P</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >6FAM-<br>TTCTCAACTCATCCCCACGTATGC-TAMRA</td></tr><tr ><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>TEM</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_TEM-F</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >CACTATTCTCAGAATGACTTGGT</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >85</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>E. coli</span> 832_16_2 *</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >[<a href="#B66-microorganisms-11-00308" class="html-bibr">66</a>]</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_TEM-R</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >TGCATAATTCTCTTACTGTCATG</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_TEM-P</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >6FAM-<br>CCAGTCACAGAAAAGCATCTTACGG-TAMRA</td></tr><tr ><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>intI1</span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_intI-F</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >GCCTTGATGTTACCCGAGAG</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >196</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>E. coli</span> pKJK5 #337</td><td rowspan='3' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >[<a href="#B67-microorganisms-11-00308" class="html-bibr">67</a>]</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_intI-R</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >GATCGGTCGAATGCGTGT</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_intI-P</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >6FAM-ATTCCTGGCCGTGGTTCTGGGTTTT-TAMRA</td></tr><tr ><td rowspan='7' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>korB</span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-F</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >TCATCGACAACGACTACAACG</td><td rowspan='7' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >118</td><td rowspan='7' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>E. coli</span> RP4 (IncP-1α)<br><span class='html-italic'>E. coli</span> JM109 pB10 (IncP-1ß)<br><span class='html-italic'>E. coli</span> <br>JM109 pQKH545 (IncP-1γ)<br><span class='html-italic'>E. coli</span> JM109 pKJK5 (IncP-1ε)</td><td rowspan='7' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >[<a href="#B58-microorganisms-11-00308" class="html-bibr">58</a>]</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-Fz</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >TCGTGGATAACGACTACAACG</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-R</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >TTCTTCTTGCCCTTCGCCAG</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-Rge</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >TTYTTCYTGCCCTTGGCCAG</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-Rd</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >TTCTTGACTCCCTTCGCCAG</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-P</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >6FAM-<br>TCAGYTCRTTGCGYTGCAGGTTCTCVAT-TAMRA</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >q_korB-Pgz</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >6FAM-<br>TSAGGTCGTTGCGTTGCAGGTTYTCAAT-TAMRA</td></tr></tbody> </table> <div class='html-table_foot html-p'><div class='html-p' style='text-indent:0em;'><span class='html-fn-content'>* clinical isolates from Robert Koch Institute Wernigerode.</span></div><div style='clear:both;'></div></div> </div><div class="html-table-wrap" id="microorganisms-11-00308-t002"> <div class="html-table_wrap_td" > <div class="html-tablepopup html-tablepopup-link" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href='#table_body_display_microorganisms-11-00308-t002'> <img alt="Table" data-lsrc="https://www.mdpi.com/img/table.png" /> <a class="html-expand html-tablepopup" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#table_body_display_microorganisms-11-00308-t002"></a> </div> </div> <div class="html-table_wrap_discription"> <b>Table 2.</b> Percentage of gene copy numbers at the end of composting, relative to the start copy numbers normalized to the 16S rRNA gene and to 1 g compost. </div> </div> <div class="html-table_show mfp-hide " id ="table_body_display_microorganisms-11-00308-t002" > <div class="html-caption" ><b>Table 2.</b> Percentage of gene copy numbers at the end of composting, relative to the start copy numbers normalized to the 16S rRNA gene and to 1 g compost.</div> <table > <thead ><tr ><th rowspan='2' align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >ARG</th><th rowspan='2' align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Normalization</th><th colspan='5' align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Decrease from Start to End of Composting [%] *</th></tr><tr ><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >E1</th><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >E1-B</th><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >E2</th><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >E2-B</th><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >Mean</th></tr></thead><tbody ><tr ><td rowspan='2' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>IMP</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >16S rRNA</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+36.1</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+52.6</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+1096.2</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+308.2</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+373.3</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >g compost</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−29.5</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−12.2</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+689.3</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−9.5</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+159.5</td></tr><tr ><td rowspan='2' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>CTX-M</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >16S rRNA</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−88.6</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+1323.3</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+499.9</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+285.8</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+505.1</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >g compost</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−94.1</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+773.9</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+245.8</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+14.1</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+234.9</td></tr><tr ><td rowspan='2' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>TEM</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >16S rRNA</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−18.1</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.8</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+66.9</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+252.5</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+50.4</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >g compost</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−56.0</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.8</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−42.1</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >+27.4</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−42.6</td></tr><tr ><td rowspan='2' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>intI1</span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >16S rRNA</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−91.6</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−92.4</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−93.2</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−82.9</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−90.0</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >g compost</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−95.7</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−95.5</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−96.1</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−96.0</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−95.8</td></tr><tr ><td rowspan='2' align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>korB</span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >16S rRNA</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.6</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.0</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−98.9</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−98.6</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.0</td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >g compost</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.7</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.3</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.6</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.5</td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >−99.5</td></tr></tbody> </table> <div class='html-table_foot html-p'><div class='html-p' style='text-indent:0em;'><span class='html-fn-content'>* signs of gene copy numbers were reversed to show increases with positive signs and decreases with negative signs.</span></div><div style='clear:both;'></div></div> </div><div class="html-table-wrap" id="microorganisms-11-00308-t003"> <div class="html-table_wrap_td" > <div class="html-tablepopup html-tablepopup-link" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href='#table_body_display_microorganisms-11-00308-t003'> <img alt="Table" data-lsrc="https://www.mdpi.com/img/table.png" /> <a class="html-expand html-tablepopup" data-counterslinkmanual = "https://www.mdpi.com/2076-2607/11/2/308/display" href="#table_body_display_microorganisms-11-00308-t003"></a> </div> </div> <div class="html-table_wrap_discription"> <b>Table 3.</b> Mean copy numbers of ARGs and horizontal gene transfer marker genes irrespective of the treatment with and without biochar, as assessed by qPCR. Copy numbers were normalized to 1 g compost. </div> </div> <div class="html-table_show mfp-hide " id ="table_body_display_microorganisms-11-00308-t003" > <div class="html-caption" ><b>Table 3.</b> Mean copy numbers of ARGs and horizontal gene transfer marker genes irrespective of the treatment with and without biochar, as assessed by qPCR. Copy numbers were normalized to 1 g compost.</div> <table > <thead ><tr ><th rowspan='2' align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >ARG</th><th colspan='2' align='center' valign='middle' style='border-top:solid thin;border-bottom:solid thin' class='html-align-center' >Mean Copy Numbers per Gram Compost</th></tr><tr ><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >Start</th><th align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >End</th></tr></thead><tbody ><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>IMP</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >5.5 × 10<sup>4</sup></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >1.0 × 10<sup>5</sup></td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>CTX-M</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >3.8 × 10<sup>4</sup></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >2.8 × 10<sup>4</sup></td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>bla<sub>TEM</sub></span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >4.1 × 10<sup>7</sup></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >3.4 × 10<sup>5</sup></td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>intI1</span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >5.0 × 10<sup>8</sup></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >2.1 × 10<sup>7</sup></td></tr><tr ><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' ><span class='html-italic'>korB</span></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >1.9 × 10<sup>7</sup></td><td align='center' valign='middle' style='border-bottom:solid thin' class='html-align-center' >7.6 × 10<sup>4</sup></td></tr></tbody> </table> </div></section><section class='html-fn_group'><table><tr id=''><td></td><td><div class='html-p'><b>Disclaimer/Publisher’s Note:</b> The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). 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margin-top: 15px;"> <div><b>MDPI and ACS Style</b></div> <p> Werner, K.A.; Feyen, L.; Hübner, T.; Brüggemann, N.; Prost, K.; Grohmann, E. Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta. <em>Microorganisms</em> <b>2023</b>, <em>11</em>, 308. https://doi.org/10.3390/microorganisms11020308 </p> <div style="display: block"> <b>AMA Style</b><br> <p> Werner KA, Feyen L, Hübner T, Brüggemann N, Prost K, Grohmann E. Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta. <em>Microorganisms</em>. 2023; 11(2):308. https://doi.org/10.3390/microorganisms11020308 </p> <b>Chicago/Turabian Style</b><br> <p> Werner, Katharina A., Lara Feyen, Tobias Hübner, Nicolas Brüggemann, Katharina Prost, and Elisabeth Grohmann. 2023. "Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta" <em>Microorganisms</em> 11, no. 2: 308. https://doi.org/10.3390/microorganisms11020308 </p> <b>APA Style</b><br> <p> Werner, K. A., Feyen, L., Hübner, T., Brüggemann, N., Prost, K., & Grohmann, E. (2023). Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta. <em>Microorganisms</em>, <em>11</em>(2), 308. https://doi.org/10.3390/microorganisms11020308 </p> </div> </div> <div class="info-box no-margin"> Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. 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Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta. <em>Microorganisms</em> <b>2023</b>, <em>11</em>, 308. https://doi.org/10.3390/microorganisms11020308 </p> <div style="display: block"> <b>AMA Style</b><br> <p> Werner KA, Feyen L, Hübner T, Brüggemann N, Prost K, Grohmann E. Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta. <em>Microorganisms</em>. 2023; 11(2):308. https://doi.org/10.3390/microorganisms11020308 </p> <b>Chicago/Turabian Style</b><br> <p> Werner, Katharina A., Lara Feyen, Tobias Hübner, Nicolas Brüggemann, Katharina Prost, and Elisabeth Grohmann. 2023. "Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta" <em>Microorganisms</em> 11, no. 2: 308. https://doi.org/10.3390/microorganisms11020308 </p> <b>APA Style</b><br> <p> Werner, K. A., Feyen, L., Hübner, T., Brüggemann, N., Prost, K., & Grohmann, E. (2023). Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta. <em>Microorganisms</em>, <em>11</em>(2), 308. https://doi.org/10.3390/microorganisms11020308 </p> </div> </div> <div class="info-box no-margin"> Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. 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