Scientific Program of Stem Cell Conference | Upcoming Stem Cell Conference Agenda

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padding-top: 0px; position: static; transform: none;"> <div class="schedule-tab"> <ul class="nav nav-tabs" id="nav-tabs"> <li class="active"><a class="animated fadeIn" href="#tab_1" data-toggle="tab">Day 1</a></li> </ul> <div class="tab-content"> <div class="tab-pane active animated fadeInRight" id="tab_1"> <h2 class="schedule-date"> Keynote Session: </h2>  <div class="row"> <div class="col-md-12"> <h2>Oral Session 1:</h2> <ul type="square"> <li>Stem Cell</li></ul> <div class="row"> </div>  <div style="border:1px solid #dadada;"></div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>09:00</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Karolynn Halpert photo" title="Karolynn Halpert" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/karolynn-halpert-chief-medical-officer-bioxcellerator-medellin-colombia" >Karolynn Halpert</a></h3> <span class="schedule-slot-affiliation">Chief Medical Officer, BioXcellerator, Medellin, Colombia</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/wharton-jelly-derived-mesenchymal-stromal-cell-wj-msc-therapy-translation-into-clinical-practice-in-orthopedics-degenerative-conditions" >Wharton Jelly derived-Mesenchymal Stromal Cell (WJ-MSC) therapy: Translation into clinical practice in orthopedics degenerative conditions</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Karolynn Halpert is a medical doctor and specialist in Emergency Medicine. She currently serves as the Chief Medical Officer and co-founder of BioXcellerator and BioXtech, where she leads scientific, medical, and research teams dedicated to translational research in regenerative medicine and advanced cellular therapy. Throughout her career, Dr. Halpert has earned a reputation for pioneering the establishment of multidisciplinary teams and strategic research platforms to advance the science of cell therapy, primarily through clinical studies using WJ-MSC to treat various conditions. Thanks to her leadership, BioXcellerator has become a global leader in cellular therapy. In fact, Dr. Halpert, and the medical and scientific teams she oversees have contributed to the company obtaining ISO certification and certification in good clinical practices. She and her team have published several articles on MSC in major scientific journals. She is also a co-founder of BioXscience, a research and innovation center focused on evidence-based knowledge development of advanced therapies. Dr. Halpert has been a recognized international speaker at many cell therapy conferences around the world</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Among some orthopedic conditions that have mostly been the target of research with ortho-biologics and regenerative medicine, it is known that the conventional treatment approach for degenerative conditions such as Knee Osteoarthritis (KOA) and Degenerative Disc Disease (DDD) focuses on alleviating symptoms through various measures, including physical therapy, weight reduction, and the use of medications to control pain and inflammation. Although current conventional therapies provide symptomatic relief, their impact on the cartilage or disc is limited. Additionally, surgical procedures such as discectomies and arthroplasties have limitations and risks. The above highlights the need to continue researching new therapies for KOA and DDD. Specifically, regenerative therapy based on ortho-biologicals can complement traditional management and has been shown in clinical studies to relieve pain, improve functionality and contribute to quality of life. The administration of ortho-biologicals, specifically from mesenchymal stromal cells (MSCs), has been shown to have a favorable impact on the process of joint and disc degeneration, given their immunomodulatory effects, as well as their effects on tissue growth, formation of collagen and differentiation into chondrocytes. In particular, studies suggest that WJ-MSC therapies are safe and well tolerated, with the potential to relieve pain and improve knee and spine functionality. These cells act in various ways, such as migration towards damaged cartilage, adaptation to their hypoxic environment, promoting the survival and differentiation of endogenous MSCs, synthesis and protection of the cartilage extracellular matrix, and exhibiting anti-inflammatory and immunomodulatory properties. It is intended to share the experience of a clinical and research center treatment with WJ-MSCs, which were delivered locally (into the knee joint and intervertebral disc) in patients with chronic degenerative orthopedic conditions. Methodology. Two retrospective cohorts were analyzed according to the effects following therapy based on WJ-MSC in KOA and DDD patients. Ethical approvals were obtained by an independent ethics committee (CEI-0435-11-2022 and CEI-0324-07-2022). Informed consent forms were signed by patients. Clinical outcomes were measured by the Short-Form12 questionnaire, Visual-Analog-Scale (VAS), and Western-Ontario-McMaster-Index (WOMAC) for KOA patients and Oswestry-DisabilityIndex (ODI) for DDD patients. KOA patients were treated with a single dose of 40x106 WJ-MSC per target knee (MSC were delivered by intra-articular route), and DDD patients were treated with 10x106 WJ-MSC per target intervertebral spine disc (MSC were delivered by intradiscally and facetally route). Previously, the allogeneic WJ-MSCs were expanded in a culture medium supplemented with 10% human platelet lysate (hPL) up to passage 7. Cell marker expression and in vitro differentiation to mesodermal lineage were verified, as well as microbiological tests. Findings. During 2022, 39 KOA patients started follow-up, of whom 26 (67%) answered the questionnaires at 6 months. There was an improvement according to pain assessment at 6 months post-therapy with an average 1.5-point reduction (↓32%) and according to pain, stiffness, and functionality assessment it was demonstrated an improvement in total WOMAC with an average reduction of 12.5 points (↓36%) in patients who had a baseline score of ≥10%. In the meanwhile, during the same period, 32 DDD patients were treated for thoracolumbar back pain and started follow-up, of whom 29 (91%) answered the questionnaires at 3 months, 30 (94%) answered at 6 months, and 22 (69%) at 12 months post-therapy. There was an improvement according to pain assessment at 6 months with an average reduction of 2.3 points (↓37%), which was maintained up to 12 months post-therapy and according to disability assessment, it was demonstrated an improvement at 6 months post-therapy with an average ODI score reduction of 9.8 points (↓29%), which was maintained until 12 months post-therapy. No adverse events were noted neither in KOA nor DDD-treated patients. Conclusion and significance. Despite the evolving nature of the evidence, the study´s findings contribute to our understanding of the therapeutic benefits of WJ-MSC in KOA and DDD-diagnosed patients, who sought treatment for failure of conservative therapy and for pain and functional limitation. The improvements evidenced involve changes in the patient's quality of life. There were no serious adverse events reported, demonstrating that these therapies are safe.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>09:40</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Leonardo Ramirez photo" title="Leonardo Ramirez" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/leonardo-ramirez-bioxcellerator-medellin-colombia" >Leonardo Ramirez</a></h3> <span class="schedule-slot-affiliation">BioXcellerator, Medellin, Colombia</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/wharton-jelly-derived-mesenchymal-stromal-cells-wj-mscs-therapy-in-orthopedics-description-of-evidence-and-experience-in-the-treatment-of-shoulder-overuse-injuries" >Wharton Jelly derived-Mesenchymal Stromal Cells (WJ-MSCs) therapy in orthopedics: description of evidence and experience in the treatment of shoulder overuse injuries.</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr. Ramirez is a physician from the University of Antioquia, Medellin, Colombia. He obtained a degree as a Specialist in basic biomedical sciences with an emphasis on human anatomy and embryology from the same university. He also had a degree as a Specialist in Emergency Medicine from CES University, Medellin, Colombia. Moreover, he obtained a master’s degree in health administration from CES University. He has more than 20 years of experience as a university professor and has served as an emergency services coordinator at various Colombian hospitals and clinics. Currently, he is a member of the Colombian Association of Specialists in Emergency and Emergency Medicine (ACEM). He has a research interest, participating as a teaching evaluator of research projects at the San Martín University Foundation in Colombia. Actually, he serves as medical director in BioXcellerator (Medellin, Colombia) a leader clinic that serves patients by offering regenerative and ortho-biological therapies. There, Dr. Ramirez carries out administrative and healthcare tasks and articulates the different areas of the clinic, focused on improving patient care.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Shoulder pain syndrome is a multifaceted condition that requires a comprehensive approach for effective management. This approach should encompass both the conventional orthopedic perspective and regenerative medicine strategies. The direct and indirect costs associated with the management of shoulder pain in primary care can be substantial, including expenses related to sick leave, medication, absenteeism from work and sports, sleep disturbances, financial burden, and delayed functional return to sports practice, among other consequences. Shoulder injuries, particularly those caused by overuse such as rotator cuff tendinopathy (RCT), impingement, labrum injuries, and bursitis, can significantly impact the quality of life of athletes and the wider population. Furthermore, even after arthroscopic surgery, re-tears can occur, leading to ongoing shoulder pain and loss of function. Managing these injuries solely through arthroscopic repair and physical therapy can be challenging in orthopedics due to issues such as poor healing, adhesion formation, and fatty tissue accumulation. The above justifies the need for research and innovation in the development of ortho-regenerative therapies that contribute, in a complementary manner to conventional orthopedic management, to the reduction of pain and functional limitation. Mesenchymal stromal cells (MSCs) are a variety of ortho-biologicals that have demonstrated considerable potential in promoting tendon remodeling and fibrocartilage formation, leading to enhanced biomechanical strength in the tendon. MSCs are characterized by their remarkable proliferation capacity, potent paracrine action, and pluripotency for the differentiation into various cell types. Moreover, the extracellular vesicles originating from MSCs can facilitate collagen production, reduce inflammation, and minimize adhesion formation by transporting regulatory proteins and microRNAs. Consequently, MSC-based therapy shows great promise as a therapeutic strategy for the healing of rotator cuff. Despite the significant surgical advancements to address rotator cuff tears, the effective connection between tendon tissue and bone remains a persistent challenge. However, MSCs offer a glimmer of hope, as they have demonstrated remarkable paracrine, anti-inflammatory, and angiogenic benefits. The MSC-based therapy, which entails growth factors and specific scaffolds, aims to enhance the biomechanical strength of the rotator cuff and promote fibrocartilage formation. While the studies have shown great promise, more research is necessary to evaluate the long-term safety and efficacy of the therapy in shoulder and elbow injuries, including clinical trials and observational studies. For this reason, we are delighted to share the experience of a clinical and research center that has treated patients with shoulder overuse injuries (SOI) such as RCT, impingement syndromes, labrum injuries, and bursitis using Wharton Jelly Derived-Mesenchymal Stromal Cells (WJ-MSCs). These MSCs were delivered locally, both into the glenohumeral joint and by intratendinous injections. Methodology. One retrospective cohort was analyzed according to the effects following therapy based on WJ-MSCsin SOI patients. Ethical approval was obtained by an institutional review board (IRB). Patients signed an informed consent form. SOI patients were treated with a single dose of 70x106 WJ-MSCs per target shoulder (MSCs were delivered 20x106by intraarticular and 50x106 by intratendinous injections) by an ultrasound-guided procedure. Previously, the allogeneic WJ-MSCs were expanded in a culture medium supplemented with 10% human platelet lysate (hPL) up to passage 7. Cell marker expression and in vitro differentiation to mesodermal lineage and microbiological tests were verified. Clinical outcomes were measured by the Short-Form12 questionnaire, Visual-Analog-Scale (VAS), and Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire at baseline (pre-therapy time), followed by 3-, 6- and 12-month measurements. Following previous literature, a responder to therapy was defined as a patient with a reduction of at least 2 points or more in the VAS score and a reduction of 10 points or more in the DASH score at the last available follow-up to baseline (pretherapy scores). Findings. From November 2021 to May 2023, 88 patients were treated at the institution and met the eligibility criteria for therapy, of which all responded to the 6-month follow-ups, and 25 (28.41%) completed the questionnaires at 12-month follow-ups. The mean age was 52.36 years old (SD 13.85). The mean body mass index (BMI) was 26.92 (SD 4.30). 84% (n= 74) were men. The most frequent country of origin was the USA (n= 68, 77%). 56% (n= 49) of patients had both shoulders treated, 20% (n= 18) received WJ-MSC in the left shoulder only and 25% (n= 21) in the right shoulder only. 47/88 patients (53.41%) responded favorably to therapy at 6 months, and 18/25 (72%) responded to therapy at 12 months. The 6-month VAS was significantly reduced compared to baseline (median baseline VAS= 5, IQR 2.25, median 6m VAS= 2, IQR 4, p< 0,001 Wilcoxon W test, p= 0,018 Shapiro-Wilk assumption-normality test), as was the 12-month VAS (median baseline VAS= 5, IQR 2.25, median 12m VAS= 2, IQR 3, p< 0,001 Wilcoxon W test, p= 0,042 Shapiro-Wilk assumptionnormality test). The 6-month DASH was significantly reduced compared to baseline (median baseline DASH= 31.25, IQR 24.99, median 6m DASH= 13.33, IQR 20.21, p< 0,001 Wilcoxon W test, p< 0,001 Shapiro-Wilk assumption-normality test), as was the 12-month DASH (median baseline DASH= 31.25, IQR 24.99, median 12m DASH= 11.67, IQR 18.34, p< 0,001 Wilcoxon W test, p= 0,011 Shapiro-Wilk assumption-normality test). The 6-month SF-12 Physical score was significantly increased compared to baseline (median baseline SF12-physical= 57.14, IQR 35.72, median 6m SF12-physical= 64.29, IQR 35.71, p <0,001 Wilcoxon W test, p= 0,047 Shapiro-Wilk assumptionnormality test), as did the 12-month SF-12 Physical score (mean baseline SF12-physical= 53.40, SD 21.50, mean 12m SF12- physical= 70.78, SD 21.06, p= 0,008 t-paired test, p= 0,064 Shapiro-Wilk assumption-normality test) and the 6-month SF12 Mental score (median baseline SF12-mental= 61.90, IQR 38.09, median 6m SF12-mental= 76.19, IQR 40.48, p <0,001 Wilcoxon W test, p= 0,011 Shapiro-Wilk assumption-normality test). No adverse events were noted.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time></time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Alejandro Ramirez photo" title="Alejandro Ramirez" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/alejandro-ramirez-bioxcellerator-medellin-colombia" >Alejandro Ramirez</a></h3> <span class="schedule-slot-affiliation">BioXcellerator, Medellin, Colombia</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/wharton-jelly-derived-mesenchymal-stromal-cell-wj-msc-therapy-in-orthopedics-description-of-evidence-and-experience-in-the-treatment-of-shoulder-overuse-injuries" >Wharton Jelly derived-Mesenchymal Stromal Cell (WJ-MSC) therapy in orthopedics: description of evidence and experience in the treatment of shoulder overuse injuries.</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Alejandro Ramirez is a medical doctor and specialist in sports medicine who graduated from the University of Antioquia, Colombia. He has worked as a sports doctor in regional teams and Colombian national cycling and soccer leagues. He has extensive knowledge in the treatment and rehabilitation of musculoskeletal injuries derived from sports and similar activities. He is currently one of the pioneering Latin American doctors in ortho-regenerative therapies, with more than 10 years of experience in applying treatments for osteoarticular injuries, especially from WJ-MSC. Over the past few years, he has honed his skills in musculoskeletal interventionism, training in ultrasound-guided procedures at the MSKUS Institute in Virginia, USA., and has also received hands-on training at the OAA Medical Center in Allentown, Pennsylvania, USA., in the orthopedic service.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Shoulder pain syndrome is a multifaceted condition that requires a comprehensive approach for effective management. This approach should encompass both the conventional orthopedic perspective and regenerative medicine strategies. The direct and indirect costs associated with the management of shoulder pain in primary care can be substantial, including expenses related to sick leave, medication, absenteeism from work and sports, sleep disturbances, financial burden, and delayed functional return to sports practice, among other consequences. Shoulder injuries, particularly those caused by overuse such as rotator cuff tendinopathy (RCT), impingement, labrum injuries, and bursitis, can significantly impact the quality of life of athletes and the wider population. Furthermore, even after arthroscopic surgery, re-tears can occur, leading to ongoing shoulder pain and loss of function. Managing these injuries solely through arthroscopic repair and physical therapy can be challenging in orthopedics due to issues such as poor healing, adhesion formation, and fatty tissue accumulation. The above justifies the need for research and innovation in the development of ortho-regenerative therapies that contribute, in a complementary manner to conventional orthopedic management, to the reduction of pain and functional limitation. Mesenchymal stromal cells (MSCs) are a variety of ortho-biologicals that have demonstrated considerable potential in promoting tendon remodeling and fibrocartilage formation, leading to enhanced biomechanical strength in the tendon. MSCs are characterized by their remarkable proliferation capacity, potent paracrine action, and pluripotency for the differentiation into various cell types. Moreover, the extracellular vesicles originating from MSCs can facilitate collagen production, reduce inflammation, and minimize adhesion formation by transporting regulatory proteins and microRNAs. Consequently, MSC-based therapy shows great promise as a therapeutic strategy for the healing of rotator cuff. Despite the significant surgical advancements to address rotator cuff tears, the effective connection between tendon tissue and bone remains a persistent challenge. However, MSCs offer a glimmer of hope, as they have demonstrated remarkable paracrine, anti-inflammatory, and angiogenic benefits. The MSC-based therapy, which entails growth factors and specific scaffolds, aims to enhance the biomechanical strength of the rotator cuff and promote fibrocartilage formation. While the studies have shown great promise, more research is necessary to evaluate the long-term safety and efficacy of the therapy in shoulder and elbow injuries, including clinical trials and observational studies. For this reason, we are delighted to share the experience of a clinical and research center that has treated patients with shoulder overuse injuries (SOI) such as RCT, impingement syndromes, labrum injuries, and bursitis using Wharton Jelly Derived-Mesenchymal Stromal Cells (WJ-MSC). These MSCs were delivered locally, both into the glenohumeral joint and by intratendinous injections. Methodology. One retrospective cohort was analyzed according to the effects following therapy based on WJ-MSC in SOI patients. Ethical approval was obtained by an institutional review board (IRB). Patients signed an informed consent form. SOI patients were treated with a single dose of 70x106 WJ-MSC per target shoulder (MSCs were delivered 20x106 by intraarticular and 50x106 by intratendinous injections) by an ultrasound-guided procedure. Previously, the allogeneic WJ-MSCs were expanded in a culture medium supplemented with 10% human platelet lysate (hPL) up to passage 7. Cell marker expression and in vitro differentiation to mesodermal lineage and microbiological tests were verified. Clinical outcomes were measured by the Short-Form12 questionnaire, Visual-Analog-Scale (VAS), and Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire at baseline (pre-therapy time), followed by 3-, 6- and 12-month measurements. Following previous literature, a responder to therapy was defined as a patient with a reduction of at least 2 points or more in the VAS score and a reduction of 10 points or more in the DASH score at the last available follow-up to baseline (pretherapy scores). Findings. From November 2021 to May 2023, 88 patients were treated at the institution and met the eligibility criteria for therapy, of which all responded to the 6-month follow-ups, and 25 (28.41%) completed the questionnaires at 12-month follow-ups. The mean age was 52.36 years old (SD 13.85). The mean body mass index (BMI) was 26.92 (SD 4.30). 84% (n= 74) were men. The most frequent country of origin was the USA (n= 68, 77%). 56% (n= 49) of patients had both shoulders treated, 20% (n= 18) received WJ-MSC in the left shoulder only and 25% (n= 21) in the right shoulder only. 47/88 patients (53.41%) responded favorably to therapy at 6 months, and 18/25 (72%) responded to therapy at 12 months. The 6-month VAS was significantly reduced compared to baseline (median baseline VAS= 5, IQR 2.25, median 6m VAS= 2, IQR 4, p< 0,001 Wilcoxon W test, p= 0,018 Shapiro-Wilk assumption-normality test), as was the 12-month VAS (median baseline VAS= 5, IQR 2.25, median 12m VAS= 2, IQR 3, p< 0,001 Wilcoxon W test, p= 0,042 Shapiro-Wilk assumptionnormality test). The 6-month DASH was significantly reduced compared to baseline (median baseline DASH= 31.25, IQR 24.99, median 6m DASH= 13.33, IQR 20.21, p< 0,001 Wilcoxon W test, p< 0,001 Shapiro-Wilk assumption-normality test), as was the 12-month DASH (median baseline DASH= 31.25, IQR 24.99, median 12m DASH= 11.67, IQR 18.34, p< 0,001 Wilcoxon W test, p= 0,011 Shapiro-Wilk assumption-normality test). The 6-month SF-12 Physical score was significantly increased compared to baseline (median baseline SF12-physical= 57.14, IQR 35.72, median 6m SF12-physical= 64.29, IQR 35.71, p <0,001 Wilcoxon W test, p= 0,047 Shapiro-Wilk assumptionnormality test), as did the 12-month SF-12 Physical score (mean baseline SF12-physical= 53.40, SD 21.50, mean 12m SF12- physical= 70.78, SD 21.06, p= 0,008 t-paired test, p= 0,064 Shapiro-Wilk assumption-normality test) and the 6-month SF12 Mental score (median baseline SF12-mental= 61.90, IQR 38.09, median 6m SF12-mental= 76.19, IQR 40.48, p <0,001 Wilcoxon W test, p= 0,011 Shapiro-Wilk assumption-normality test). No adverse events were noted. Conclusion and significance. This study´s findings contribute to our understanding of the therapeutic benefits of WJ-MSC in SOI-diagnosed patients, who sought treatment for failure of conservative therapy and pain and functional limitation. The improvements evidenced involve changes in the patient's quality of life. There were no serious adverse events reported, demonstrating that this therapy is safe.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>11:10</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Sudha Bansode photo" title="Sudha Bansode" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/sudha-bansode-shankarrao-mohite-mahavidyalaya-india" >Sudha Bansode</a></h3> <span class="schedule-slot-affiliation">Shankarrao Mohite Mahavidyalaya, India</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/introduction-to-stem-cell-and-its-activity" >Introduction to Stem Cell and its Activity</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr. Sudha Bansode is a Professor in Zoology at Shankarrao Mohite College, Akluj, and Maharashtra State, India. Recently she has completed her Post Doctoral Studies at University of California, Riverside, USA. She is a active researcher & passionate teacher in India. Still she has been published above 25 research papers in International Journals & she is interested on Bone Research. Also she has honor of Distinguished Editorial Board Member of several International Journals. She is a own author of “Textbook Histological Techniques” & “Outlines of Physiology”. And now she is working on another own reference book “Rhythms in Freshwater Crustaceans”. She is a University recognized research guide for Ph. D. students in India. She was a invited Indian Speaker of “OXFORD SYMPOSIUM” on27-29 August, 2014 at Balliol College, Oxford, United Kingdom & CELL SIGNALING & CANCER THERAPY – International Conference at Double Tree, Hilton Chicago on 27-28 September 2017. She was academic visitor of Bangkok- Thailand, Colombo-Sri Lanka, Daira-Daira-Dubai-UAE. Her recent intellectual Interaction is with many International Professional groups.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> The general designation, “stem cell” encompasses many distinct cell types. Commonl, the modifiers, “embryonic,” and “adult” are used to distinguish stem cells by the developmental stage of the animal from which they come, but these terms are becoming insufficient as new research has discovered how to turn fully differentiated adult cells back into embryonic stem cells and, conversely, adult stem cells, more correctly termed “somatic” stem cells meaning categories based on their biologic properties - pluripotent stem cells and multipotent stem cells. Their sources, characteristics, differentiation and therapeutic applications are discussed.</p><p style="text-align: justify;"> From the body, are found in the fetus, placenta, umbilical cord blood and infants.<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104807/#R2" target="_blank"><sup>2</sup></a> Therefore, this review will sort stem cells into two Stem cells have the ability to differentiate into specific cell types. The two defining characteristics of a stem cell are perpetual self-renewal and the ability to differentiate into a specialized adult cell type. There are two major classes of stem cells: pluripotent that can become any cell in the adult body, and multipotent that are restricted to becoming a more limited population of cells. Cell sources, characteristics, differentiation and therapeutic applications are discussed. Stem cells have great potential in tissue regeneration and repair but much still needs to be learned about their biology, manipulation and safety before their full therapeutic potential can be achieved.</p><p style="text-align: justify;"> Stem cells have the ability to build every tissue in the human body, hence have great potential for future therapeutic uses in tissue regeneration and repair. In order for cells to fall under the definition of “stem cells,” they must display two essential characteristics. First, stem cells must have the ability of unlimited self-renewal to produce progeny exactly the same as the originating cell. This trait is also true of cancer cells that divide in an uncontrolled manner whereas stem cell division is highly regulated. Therefore, it is important to note the additional requirement for stem cells; they must be able to give rise to a specialized cell type that becomes part of the healthy animal</p><p>  </p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>11:50</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Luiz Eduardo Imbelloni photo" title="Luiz Eduardo Imbelloni" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/luiz-eduardo-imbelloni-senior-researcher-at-the-national-cancer-institute-brazil" >Luiz Eduardo Imbelloni</a></h3> <span class="schedule-slot-affiliation">Senior Researcher at the National Cancer Institute, Brazil</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/spinal-anesthesia-puncture-position-and-local-anesthetic-solutions-better-understanding-for-better-indication" >Spinal anesthesia, puncture position and local anesthetic solutions. Better understanding for better indication</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr. Luiz Eduardo Imbelloni, Senior Researcher at the National Cancer Institute in Brazil.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Two schools teach spinal anesthesia, one prefers the lateral decubitus position and the other the sitting position. The vast majority of anesthesiologists worldwide use routinely the hyperbaric solution of bupivacaine for almost all types of surgery, mainly performed in a sitting position. There is renewed interest in understanding spinal anesthesia about puncture position, the various solutions used, and the position of the operating table during the surgical procedure. Understanding the vertebral column, of the anterior and posterior roots, allows the different puncture positions and the hyperbaric, isobaric, and hypobaric solutions to offer better quality of these techniques and will be shown in this article. The application of the three solutions in different puncture positions, and the position of the surgical table during the procedure, will be shown in seven items in 14 tables, and 6 figures, for the complete understanding of spinal anesthesia. The possibility of performing hemispinal anesthesia (unilateral and posterior), and the possibility of performing completely sensory spinal anesthesia without any degree of motor block are discussed. The explanations in this article with the different puncture positions and the three local anesthetic solutions are applied both in the lumbar region and in the thoracic region.</p><p>  </p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>12:40</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Jean Mercier Ythier photo" title="Jean Mercier Ythier" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/jean-mercier-ythier-sorbonne-university-france" >Jean Mercier Ythier</a></h3> <span class="schedule-slot-affiliation">Sorbonne University, France.</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/who-owns-hematopoietic-stem-cells-some-fundamental-legal-stakes-for-the-manufacture-of-cultured-red-blood-cells-an-update" >Who Owns Hematopoietic Stem Cells? Some Fundamental Legal Stakes for the Manufacture of Cultured Red Blood Cells: An update</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr.Jean Mercier Ythier is working as a professor in Sorbonne University in France.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Significant R&D investment is currently being made in the design of cost-effective devices for the manufacture of red blood cells (RBCs) from hematopoietic stem cells. Recent technological advances open perspectives for the for-profit manufacture of homologous transfusion products for alloimmunized patients, at production costs less than 1,500 euros/cRBC unit in realistic scale facilities. Stem cell lines from scarce phenotypes of universal donors will gain substantial economic value as basic assets of this new bio-industry. This paper addresses a number of issues related to this predictable consequence of the development of an RBC industry. The main strands of case law and legal literature related to cell line patents are reviewed and their applicability to putative RBC manufacturing is discussed. I examine the various conceivable patterns of compensation for stem cell donors, from full-market compensation to full-free (i.e., uncompensated) donation, and discuss their respective relevance for the collection of suitable cell lines and for the development of the industry.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>14:30</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Markus Bredel photo" title="Markus Bredel" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/markus-bredel-university-of-alabama-at-birmingham-usa" >Markus Bredel</a></h3> <span class="schedule-slot-affiliation">University of Alabama at Birmingham, USA</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/novel-egfr-ectodomain-mutations-and-resistance-to-anti-egfr-and-radiation-therapy-in-h-n-cancer-stem-cells" >Novel EGFR Ectodomain Mutations and Resistance to anti-EGFR and Radiation Therapy in H&N Cancer Stem Cells</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr. Markus Bredel is working at Department of Radiation Oncology and Department of Biochemistry and Molecular Genetics (WJP), University of Alabama at Birmingham, Birmingham, Alabama, USA.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Purpose: EGFR-targeted monoclonal antibodies (mAbs) provide clinical benefit in some patients with H&N squamous cell carcinoma (HNSCC), but others progress with minimal response. Missense mutations in the EGFR ectodomain (ECD) can be acquired under mAb therapy by mimicking the effect of large deletions on receptor untethering and activation. Little is known about the contribution of EGFR ECD mutations to EGFR activation and anti-EGFR response in HNSCC. Methods: We selected patient-derived HNSCC cells (UM-SCC-1) for resistance to mAb Cetuximab (CTX) by repeated, stepwise exposure to mimic what may occur clinically and identified two concurrent EGFR ECD mutations (UM-SCC-1R). We examined the competence of the mutants to bind EGF ligand or CTX. We assessed the potential impact of the mutations through visual analysis of space-filling models of the native sidechains in the original structures vs. their respective side-chain mutations. We performed CRISPR in combination with site-directed mutagenesis to test for the effect of the mutants on ligandindependent EGFR activation and sorting. We determined the effects on receptor internalization, endocytosis, downstream signaling, and radiation sensitivity. Results: UM-SCC-1R cells carried two non-synonymous missense mutations (G33S and N56K) mapping to domain I in or near the EGF binding pocket of the EGFR ECD. Structural modeling predicted that these mutants restrict the adoption of a tethered, inactive EGFR conformation while not permitting association of EGFR with the EGF ligand or CTX. Binding studies confirmed that the mutant, untethered receptor displayed a reduced affinity for both EGF and CTX but demonstrated sustained activation and presence at the cell surface with diminished internalization and sorting for endosomal degradation. Single and double-mutant models demonstrated that the G33S mutant is dominant over the N56K mutant in its effect on EGFR activation and EGF binding. CTX-resistant UM-SCC-1R cells demonstrated cross-resistance to mAb Panitumuab but, paradoxically, remained sensitive to the reversible receptor tyrosine kinase inhibitor Erlotinib. Conclusions: HNSCC cells can select for EGFR ECD mutations under EGFR mAb exposure that converge to trap the receptor in an open, constitutively activated state. These mutants impede the receptor’s competence to bind mAbs and EGF ligand and alter its endosomal trafficking, possibly explaining certain cases of clinical mAb and radiation resistance.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>12:00</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Giuseppe Gullo photo" title="Giuseppe Gullo" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/giuseppe-gullo-university-of-padua-r-n" >Giuseppe Gullo</a></h3> <span class="schedule-slot-affiliation">University of Padua. </span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/fertility-preservation-stem-cells-in-the-denatality-era" >Fertility Preservation & Stem Cells In the Denatality Era</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><div style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small; background-color: rgb(255, 255, 255); text-align: justify;"> <p> Dr. Giuseppe Gullo finished his medical school in 2007 and the Residency program in obstetrics and gynecology at the University of Palermo in 2013. He performed his PhD in Medical and Surgical Biotechnology of Human Reproduction in 2017 at University of Messina.He worked at the I. V. F.Centre—IAKENTRO Thessaloniki in September–October 2010 and as PhD student in 2016 performing Ph.D thesis.<br /> At the I. V. F. & Fetal Medicine King’s College Hospital  - London from November 2010 to February 2011,than in Yale Fertility Centre, New Haven, USA (May 2011) and he performed Endoscopic Surgery  (Laparoscopic-Hysteroscopy and Laparotomy ) at Sacred Heart Hospital Don Calabria—Negrar (Verona) from July 2011 to August 2012.In 2023 he performed a II level Master in reproductive <a href="http://medicine.at/" target="_blank">medicine.at</a> University of Padua.He is member of National and International Scientific Societies in Obstetrics-Gynecology including SIGO, SIGITE, ISGE, SIFIOG, SIGO, AOGOI, and ESHRE. He is an authorand co-author of more than 80 papers in national and international journals.</p></div><p>  </p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Fertility preservation is an emerging field in medicine that enables men, women, and children to maintain reproductive health when it is threatened by gonadotoxic treatment or no oncologic malignancies that can impair spermatogenesis and ovogenesis. Established methods include sperm cryopreservation or experimental testicular tissue cryopreservation for males, and oocyte cryopreservation or ovarian tissue cryopreservation for females. Fertility preservation treatments must be addressed through a multidisciplinary approach that involves gynecologists, urologists, oncologists, paediatricians, and professionals in the field of medically assisted reproduction.<br /> In this denantality ERA a significant gaps in knowledge about age-related fertility decline, and egg cryopreservation conditions and its complications. It is crucial to impart to these women a better knowledge about fertility especially on the number of high-quality retrieved mature oocytes and live birth rates depend on women's age. </p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>12:30</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Alessandra Lopez photo" title="Alessandra Lopez" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/alessandra-lopez-university-of-padua-r-n" >Alessandra Lopez</a></h3> <span class="schedule-slot-affiliation">University of Padua. </span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/fertility-preservation-stem-cells-in-the-denatality-era" >Fertility Preservation & Stem Cells In the Denatality Era</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Alessandra Lopez performed her Medical School at University of Palermo in 2017 and her residency programm (2019-2024) performing a thesis on COVID IMPACT on reproductive medicine at AOOR VILLA SOFIA CERVELLO Hospital-University of Palermo.She is author of many paper and actually she is following a II level Master Degree on reproductive medicine at University of Padua. </p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p> Fertility preservation is an emerging field in medicine that enables men, women, and children to maintain reproductive health when it is threatened by gonadotoxic treatment or no oncologic malignancies that can impair spermatogenesis and ovogenesis. Established methods include sperm cryopreservation or experimental testicular tissue cryopreservation for males, and oocyte cryopreservation or ovarian tissue cryopreservation for females. Fertility preservation treatments must be addressed through a multidisciplinary approach that involves gynecologists, urologists, oncologists, paediatricians, and professionals in the field of medically assisted reproduction.</p><p> In this denantality ERA a significant gaps in knowledge about age-related fertility decline, and egg cryopreservation conditions and its complications. It is crucial to impart to these women a better knowledge about fertility especially on the number of high-quality retrieved mature oocytes and live birth rates depend on women's age. </p><p>  </p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>16:00</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Ayala Vazquez Natalia photo" title="Ayala Vazquez Natalia" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/ayala-vazquez-natalia-austral-university-city-of-buenos-aires-argentina" >Ayala Vazquez Natalia</a></h3> <span class="schedule-slot-affiliation">Austral University, City of Buenos Aires, Argentina</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/lumbar-intradiscal-stem-cells-implant-for-discogenic-pain-our-experience-in-20-patients" >Lumbar intradiscal stem cells implant for discogenic pain: our experience in 20 patients</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Natalia Ayala Vazquez , MD and neurosurgeon currently medical director of PNL stem cells in Buenos Aires (pain and longevity) with experience in improving patients quality of life by implementing regenerative medicine in her daily practice. Specialized in Neurosurgery, Pain medicine and biological therapies she combines these 3 main areas to treat and prevent frequent pathologies focusing on longevity and patients well being. Furthermore she is a passionate professor in Pain medicine, international instructor in simulation and part of the academic commitee that coordinates medical students and profesors at Austral University, Argentina. </p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Introduction & Statement of the problem: Low back pain is one of the most frequent pains we see in our daily practice. It can have different etiologies and disc degeneration is one of the main ones leading to discogenic pain. The use of mesenchymal stem cells (MCSs) has increasingly scoped within the field of regenerative medicine and in recent years they have begun to be implanted in degenerative lumbar spinal discs with promising results. The evidence is still low grade of recommendation. We have gathered an n of 20 patients who went through this treatment with the aim of improving their pain, functionality and slowing down the catabolic and degenerative disc cascade or if possible regenerating partially or totally the disc. We developed a work protocol and want to show our experience and contribute with our results to the scientific community. Methodology: Based on clinical and imaging diagnosis we carried out a rigorous selection of patients following inclusion and exclusion criterias. We scheduled the procedure in the surgical room between March and December 2023. Under neuroleptoanesthesia, with strict asepsis and antisepsis measures, we performed an aspiration of 80mL of bone marrow (BMA) from the iliac crest. With 4 syringes of 20mL and propelling the embolus appropriately we extracted BMA. Subsequently, this material was centrifuged at 2400RPM/min for 10 min to produce a 30mL bone marrow concentrate (BMAC). From this amount, we precisely extracted the buffy coat and plasma with 10ml syringes and a 50/8 needle obtaining a total amount between 13 to 16mL. Guided by fluoroscopy, we injected 1,5 to 2mL of this concentrate rich in stem cells (BMSCs) into the pulposus nucleus of each degenerated lumbar intervertebral disc with a Pfirrmann grade between I and IV, regardless of Modic grade (1, 2 or 3). After the implant, the patients continued to be assessed in three main parameters: a) reduction of pain using the Owestry index at 3, 6 and 12 months b) functionality evaluated in kinesiology and using the SF 36 scale at 3, 6 and 12 months c) Lumbosacral MRI without contrast between 10 to 12 months post implant. Results: In our n of 20 patients we saw improvements in at least two of the 3 proposed objectives within a year of the treatment. 70% (n=14) improved their pain and functionality after 3 months. 15% (n=3) achieved improvement in their pain and functionality at 6 months. The remaining 15% did not improve after 3, 6 or 12 months. Only 2 patients have reached 10 months of follow-up and only in one we have observed minimal intradiscal changes, which might show encouraging results.</p><p style="text-align: justify;"> Conclusion: In our experience, mesenchymal stem cells extracted from BMAC and applied into degenerative lumbar intervertebral discs in selected patients with discogenic pain show promising results, generating improvement in the pain and functionality of the patients. 85% showed improvement in pain and functionality at 6 months. We showed that all patients who improved their functionality also improved their pain at 3 and 6 months. Signs of intradiscal rehydration on MRI is a parameter that we cannot yet evaluate since our patients do not have the described sufficient follow-up time to observe changes in them (10 to 18 months). We may conclude given the above results, that the well known immunomodulating effect of MCSs (growth factors, proangiogenic, antiapoptotic, chemoattraction, others) are seen in clinical outcome (reduction of pain and improvement of functionality) long before any changes and signs of rehydration in MRI are yet observed.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time></time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Ficcadenti Santiago photo" title="Ficcadenti Santiago" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/ficcadenti-santiago-uba-city-of-buenos-aires-argentina" >Ficcadenti Santiago</a></h3> <span class="schedule-slot-affiliation">UBA, City of Buenos Aires, Argentina.</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/lumbar-intradiscal-stem-cells-implant-for-discogenic-pain-our-experience-in-20-patients" >Lumbar intradiscal stem cells implant for discogenic pain: our experience in 20 patients</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> <span style="color: rgba(0, 0, 0, 0.9); font-family: -apple-system, system-ui, BlinkMacSystemFont, "Segoe UI", Roboto, "Helvetica Neue", "Fira Sans", Ubuntu, Oxygen, "Oxygen Sans", Cantarell, "Droid Sans", "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Emoji", "Segoe UI Symbol", "Lucida Grande", Helvetica, Arial, sans-serif; font-size: 14px; white-space-collapse: preserve-breaks; background-color: rgba(0, 0, 0, 0.08);">Santiago Ficcadenti is a MD & Anesthesiologist at PNL Stem Cells (Buenos Aires, Argentina) with experiencie in improving patient’s quality of life through Regenerative Medicine. Specializing in Anesthesia & biological therapies, uses that experience to improve patients longevity and global wellness. Aiming to balance his two passions: Medicine & Sports, when he is not at surgery or consulting room, he is an enthusiastic athlete trying to improve his fitness and general well-being.</span></p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Introduction & Statement of the problem: Low back pain is one of the most frequent pains we see in our daily practice. It can have different etiologies and disc degeneration is one of the main ones leading to discogenic pain. The use of mesenchymal stem cells (MCSs) has increasingly scoped within the field of regenerative medicine and in recent years they have begun to be implanted in degenerative lumbar spinal discs with promising results. The evidence is still low grade of recommendation. We have gathered an n of 20 patients who went through this treatment with the aim of improving their pain, functionality and slowing down the catabolic and degenerative disc cascade or if possible regenerating partially or totally the disc. We developed a work protocol and want to show our experience and contribute with our results to the scientific community. Methodology: Based on clinical and imaging diagnosis we carried out a rigorous selection of patients following inclusion and exclusion criterias. We scheduled the procedure in the surgical room between March and December 2023. Under neuroleptoanesthesia, with strict asepsis and antisepsis measures, we performed an aspiration of 80mL of bone marrow (BMA) from the iliac crest. With 4 syringes of 20mL and propelling the embolus appropriately we extracted BMA. Subsequently, this material was centrifuged at 2400RPM/min for 10 min to produce a 30mL bone marrow concentrate (BMAC). From this amount, we precisely extracted the buffy coat and plasma with 10ml syringes and a 50/8 needle obtaining a total amount between 13 to 16mL. Guided by fluoroscopy, we injected 1,5 to 2mL of this concentrate rich in stem cells (BMSCs) into the pulposus nucleus of each degenerated lumbar intervertebral disc with a Pfirrmann grade between I and IV, regardless of Modic grade (1, 2 or 3). After the implant, the patients continued to be assessed in three main parameters: a) reduction of pain using the Owestry index at 3, 6 and 12 months b) functionality evaluated in kinesiology and using the SF 36 scale at 3, 6 and 12 months c) Lumbosacral MRI without contrast between 10 to 12 months post implant. Results: In our n of 20 patients we saw improvements in at least two of the 3 proposed objectives within a year of the treatment. 70% (n=14) improved their pain and functionality after 3 months. 15% (n=3) achieved improvement in their pain and functionality at 6 months. The remaining 15% did not improve after 3, 6 or 12 months. Only 2 patients have reached 10 months of follow-up and only in one we have observed minimal intradiscal changes, which might show encouraging results.</p><p style="text-align: justify;"> Conclusion: In our experience, mesenchymal stem cells extracted from BMAC and applied into degenerative lumbar intervertebral discs in selected patients with discogenic pain show promising results, generating improvement in the pain and functionality of the patients. 85% showed improvement in pain and functionality at 6 months. We showed that all patients who improved their functionality also improved their pain at 3 and 6 months. Signs of intradiscal rehydration on MRI is a parameter that we cannot yet evaluate since our patients do not have the described sufficient follow-up time to observe changes in them (10 to 18 months). We may conclude given the above results, that the well known immunomodulating effect of MCSs (growth factors, proangiogenic, antiapoptotic, chemoattraction, others) are seen in clinical outcome (reduction of pain and improvement of functionality) long before any changes and signs of rehydration in MRI are yet observed.</p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time></time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Rachel Castro Teixeira dos Santos photo" title="Rachel Castro Teixeira dos Santos" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/rachel-castro-teixeira-dos-santos-federal-university-of-minas-gerais" >Rachel Castro Teixeira dos Santos</a></h3> <span class="schedule-slot-affiliation">Federal University of Minas Gerais</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/ipsc-derivation-to-recover-threatened-wild-species-from-reviewing-techniques-for-obtaining-ipscs-to-comparing-induction-outcomes-in-avian-and-human-cells" >iPSC Derivation to Recover Threatened Wild Species - From reviewing techniques for obtaining iPSCs to Comparing Induction Outcomes in Avian and Human Cells</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Rachel Castro Teixeira dos Santos is engaged in research in the Cellular Biology laboratory at the Institute of Biological Sciences of the Federal University of Minas Gerais through the Institutional Program of Scholarships for Technological Development and Innovation (PIBIT). She began her research career while still in high school, being awarded at four higher education institutions: Federal Institute of Education, Science and Technology of Southeastern Minas Gerais (IFET), the Federal University of Juiz de Fora (UFJF), the Federal University of Minas Gerais (UFMG), and the University of São Paulo (USP). She worked in the field of Microbiology at the Laboratory of Biotherapeutic Agents (LABio) at the Institute of Biological Sciences of UFMG through PIBIT (2021 – 2022). She was a student of the Summer Course in Human Reproduction at the Fertility Clinic in São Paulo, where she later served as a monitor (2022-2023) and worked as an intern at the CELL Clinic – Assisted Human Reproduction (2023). Currently, she is working on the research project "Reproductive Biotechnologies with iPSC Generation for the Recovery of Endangered Avian Species" in the area of Cellular Biology at the Department of Morphology at the Institute of Biological Sciences of UFMG.Iara Pastor Nogueira is currently a doctoral candidate in the Graduate Program in Cellular Biology at the Institute of Biological Sciences (ICB) of the Federal University of Minas Gerais. She holds a Master's degree in Physiology from the Graduate Program in Biological Sciences: Physiology and Pharmacology of the ICB (2020), as well as from the Department of Physiology at Western University – Ontario – Canada, through the ELAP Program (Emerging Leaders of the Americas Program – 2019). Abroad, she also participated in the Science Without Borders program, completing the final year of the Marine Biology course at Swansea University – United Kingdom. Through this program, she interned in the Veterinary Medicine sector of the Lisbon Oceanarium – Portugal. She presented two papers as the first author at the YABOUMBA congress in Paris – France.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Induced pluripotent stem cells (iPSCs) were first generated by Yamanaka in 2006, revolutionizing research by overcoming limitations imposed by the use of embryonic stem cells. In terms of the conservation of endangered species, iPSC technology presents itself as a viable alternative for the manipulation of target genetics without compromising specimens. Although iPSCs have been successfully generated for various species, their application in nonmammalian species, particularly avian species, requires further in-depth investigation to cover the diversity of wild species at risk and their different protocol requirements. This study aims to provide an overview of the workflow for iPSC induction, comparing well-established protocols in humans and mice with the limited information available for avian species. In addition to the review, the use of lipid nanoparticles LNP4 for inducing pluripotent stem cells in avian and human cells will be discussed.Gene delivery via LNP4 is non-integrative, providing greater biosafety and better performance compared to methods using cationic lipids. It promotes increased delivery of DNA into the cell nucleus, higher cell viability, and exhibits lower immunogenicity compared to other cell transfection methods. Here, we discuss the somatic cell sources to be reprogrammed, genetic factors, delivery methods, enhancers, a brief history of achievements in avian iPSC derivation, the main approaches for iPSC characterization, laboratory results regarding transfection using LNP4 in nonmammalian origin cells (Feather follicle Cells – FFCs – derived from <em>Gallus gallus </em>and <em>Anodorhynchus hyacinthinus</em>) and human origin cells (spontaneously immortalized keratinocytes - HaCaT) and the future perspectives and challenges for the field. By examining the current protocols and state-of-the-art techniques employed in iPSC generation, we seek to contribute to the development of efficient and species-specific iPSC methodologies for at-risk avian species. The advancement of iPSC technology holds great promise for achieving in vitro germline competency and, consequently, addressing reproductive challenges in endangered species, providing valuable tools for basic research, bird genetic preservation and rescue, and the establishment of cryobanks for future conservation efforts.</p></div> </div> </div> </div> </div> <h2>Oral Session 2:</h2> <ul type="square"> <li>Regenerative Medicine</li></ul> <div class="row"> </div>  <div style="border:1px solid #dadada;"></div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>13:40</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Harish Aryal photo" title="Harish Aryal" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/harish-aryal-marymount-university-the-united-states" >Harish Aryal</a></h3> <span class="schedule-slot-affiliation">Marymount University, The United States</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/high-purity-germanium-detector-for-gamma-spectroscopy-to-aid-real-time-imaging-stem-cells" >High Purity Germanium Detector for Gamma Spectroscopy to aid Real-Time Imaging STEM Cells</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr. Aryal is a Tenure-Track Assistant Professor in the College of Sciences and Humanities, School of Science, Mathematics, and Engineering at Marymount University since Spring 2023 prior to MU he was an Assistant Professor at the University of Texas-Permian Basin from 2020-2022. He holds Ph.D. in Nuclear Engineering (2020), two masters’ in mechanical (2016) and Nuclear Engineering (2014), and Bachelor’s in Nuclear Engineering with a Physics minor (2012), all degrees from ABET-accredited universities from the States with High Honours. His expertise involves the study of heat/mass transfer, and fluid mechanics processes in nuclear-mechanical systems. Key sub-areas: phase change, single/multi-phase fluids, <em>HTGR, MSR, LMFBR, LWR using RELAP, CFD, MCNP, Scale, multi-physics, PRA, Mathematica, SAPHIRE, OpenBUGS, Fusion 360</em>. Has grant experience with NSF, DoEd, DOE, NRC, and DOD (and has experience in modeling and simulation of reactor systems, radiation shielding, medical imaging, nuclear security, and non-proliferation. Currently, his lab involves creating renewable energy-Engr projects tailored to historically underrepresented cohorts at MU. Another avenue includes the use of radioisotopes in nuclear medicine image reconstruction/applications to support real-time imaging.</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><div> <p style="text-align: justify;"> Gamma spectroscopy involves analyzing the energy spectrum of gamma rays emitted by targeted atoms present in the sample to be identified. It uses detectors to capture and measure these gamma rays, producing detailed information about the levels of radioactivity present in a sample and its composition. The commonly available detectors are high-purity germanium (HPGe) gamma spectrometer, LaBr3 gamma spectrometer, and NaI (Tl) gamma spectrometer of which semiconductor-based HPGe’s are the most advanced types due to their extensive range of energies and are used for a variety of applications. However, cryogenic cooling is required for such semiconductor-based detectors and two options are available, the first option cooling via standard liquid Nitrogen and the second method by integrated electro-mechanical cryocoolers (ICS). This liquid Nitrogen-free ICS employs the latest generation technology and is now available in two versions, the ICS-E model and the ICS-P4 PopTop model. Both ICS models are highly reliable, have low maintenance, and are mechanically cooled; however, the proposed ICS-P4 PopTop model has better compatibility is portable, and can deliver superior performance in executing transformative multi-disciplinary research.<br /> This state-of-the-art Spectrometer enables us to study <strong>transport processes in syncytia: </strong>A syncytium, or a multinucleated tissue sharing a cytoplasm, requires various transport pathways (both active and passive) to allow for RNA, protein, and other biological molecules to move throughout the structure. Skeletal muscle, cardiac muscle, smooth muscle, syncytiotrophoblast in placenta, and fungi in yeast are examples of syncytium. At MU, research is skeletal muscle engineering. In skeletal muscle, the localizations, mechanisms of transport, and roles of these localized pools of RNA, protein, and other biological molecules throughout the structure of syncytium are yet to be elucidated (Pinheiro et al, 2021; Danes et al, 2021, Bagley et al, 2023).<br /> Several techniques have been utilized to assess the localizations of RNA, protein, and other biological molecules (immunolabeling, fluorescence reporter tagging, in-situ hybridization, and single-cell sequencing). However, several of these techniques cannot be utilized in a real-time fashion, such as in-situ hybridization utilized by both Pinheiro et al, 2021 and Danes et al, 2021, in which only a snapshot is visualized using fixed, permeabilized fibers. Techniques such as the MS2 system, as utilized by Danes et al, 2021, allow for live cell RNA dynamics to be assessed. However, it requires genetic manipulation, with high costs and technical demands. Dyes that label RNA such as SytoRNA Select allow for labeling of RNA in real time. However, the specificity of this labeling and the signal-to-noise ratio is limited as it can also bind to DNA.<br /> We propose to utilize radioactively labeled amino acids (such as 3H-Leucine), and <sup>32</sup>P and <sup>32</sup>S nucleotides to assess the spatial distribution of proteins and RNA in syncytium (i.e. skeletal muscle) to characterize transport processes. Specifically, we will elucidate the mechanisms of transport as skeletal muscle precursor cells (C2C12 cells, a murine model of myoblasts) fuse to form multinucleated myotubes. We will be measuring the uptake of these radionuclides using the gamma-ray spectrometer (HPGe-ICS P4). We will extend this work by characterizing the impact of proteins in various pathways (such as YAP/TAZ in Hippo pathway, and microtubules) by genetic and pharmacological manipulations. Furthermore, we will assess how estrogen and other hormones impact this process through radiolabeling of estrogen and other hormones  [18F]-fluoroestradiol ([18F]-F-FES).<br /> This research will be further expanded to look at the impact of transport processes in a bacterial biofilm. <em>Staphylococcus epidermidis</em> is susceptible to the antibiotic vancomycin in a planktonic state. However, in a biofilm, 75% of these bacteria are resistant to the same antibiotic.  The transport mechanisms in this biofilm are still being elucidated. We will further examine this mechanism by utilizing radioactive labeling.  This resistance mechanism will be further elucidated using [18F]-fluorodeoxyglucose ([18F]-FDG),[18F]-fluoroestradiol ([18F]-F-FES),  radioactively labeled amino acids (such as 3H-Leucine), and <sup>32</sup>P and <sup>32</sup>S nucleotides in bacterial biofilms.</p></div><p>  </p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>15:30</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Paulo Cezar Schutz photo" title="Paulo Cezar Schutz" class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/paulo-cezar-schutz-dr-paulo-cezar-schutz-clinic-brazil-1692206370" >Paulo Cezar Schutz</a></h3> <span class="schedule-slot-affiliation">Dr.Paulo Cezar Schutz Clinic, Brazil</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/orthobiologics-and-regenerative-medicine-to-treat-osteoarticular-pain" >Orthobiologics and Regenerative Medicine to Treat Osteoarticular Pain</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr. <span style="text-align: justify;">Paulo Cezar Schutz is working as a Orthopaedic Surgeon.in Brazil. He done his masters degree in Moelcular genetic diagnosis.</span></p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> <span style="text-align: justify;">The increasing burden of musculoskeletal disorders combined with the high utilization of opiates, NSAIDs and the relatively limited ability of traditional approaches to satisfactorily address many of the conditions that causes osteoarticular pain has spurred an increased interest in alternative treatments such as regenerative medicine therapies. Osteoarticular pain is a critical health, social, and economic issue in modern societies. Evidence is growing to support the use of orthobiologic injection treatments. Regenerative injection-based therapy has established itself as a therapeutic option for the management of osteoarthritis pain for most orthopedic conditions using platelet-rich plasma (PRP), bone marrow aspirate concentrate (BMAC), bone marrow aspirate (BMA) and mesenchymal stem cell from fat (biofat). This conference will show the results of this therapies in a sample of Brazilian patients with low back pain involving the muscle-ligament-facet-discal  complex, and other orthopedic degenerative conditions and osteoarthritis of joints.The increasing burden of musculoskeletal disorders combined with the high utilization of opiates, NSAIDs and the relatively limited ability of traditional approaches to satisfactorily address many of the conditions that causes osteoarticular pain has spurred an increased interest in alternative treatments such as regenerative medicine therapies. Osteoarticular pain is a critical health, social, and economic issue in modern societies. Evidence is growing to support the use of orthobiologic injection treatments. Regenerative injection-based therapy has established itself as a therapeutic option for the management of osteoarthritis pain for most orthopedic conditions using platelet-rich plasma (PRP), bone marrow aspirate concentrate (BMAC), bone marrow aspirate (BMA) and mesenchymal stem cell from fat (biofat). This conference will show the results of this therapies in a sample of Brazilian patients with low back pain involving the muscle-ligament-facet-discal  complex, and other orthopedic degenerative conditions and osteoarthritis of joints.</span></p></div> </div> </div> </div> </div> <div class="schedule-listing"> <div class="row schedule-item"> <div class="col-md-2"><time>14:10</time></div> <div class="col-md-10"> <div class="schedule-slot-info"> <div class="speaker"> <img src="https://d2cax41o7ahm5l.cloudfront.net/" alt="Meetings International - Stem Cell 2025 Conference Keynote Speaker Greice Cristina Nitschke Fontana photo" title="Greice Cristina Nitschke Fontana " class="schedule-slot-speakers"/> </div> <h3 class="schedule-slot-title"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/speaker/greice-cristina-nitschke-fontana-dr-paulo-cezar-schutz-clinic-brazil" >Greice Cristina Nitschke Fontana </a></h3> <span class="schedule-slot-affiliation">Dr.Paulo Cezar Schutz Clinic, Brazil</span> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="bio-inte"> <h4 class="schedule_speaker_head"><strong>Title: </strong><span class="schedule-slot-speaker-name"><a target="_blank" href="https://www.meetingsint.com/conferences/stemcellresearch/2024/abstract/the-importance-of-the-soil-for-the-regenerative-treatment" >The importance of the â€œsoilâ€ for the Regenerative Treatment</a><span></h4> <h4 class="schedule_speaker_head"><strong>Biography:</strong></h4> <div class="schedule-speaker-biography-content"><p style="text-align: justify;"> Dr.Greice Cristina Nitschke Fontana is an Obstetrician-gynecologist, OB/GYN Sonographer specialist and neurologist in Brazil. Member of International Society of Neuro Psycho Physical Optimization with REAC Technology. Attending post-graduation course in Regenerative Medicine - Orthoregen International Course</p></div> <h4 class="schedule_speaker_head"><strong>Abstract:</strong></h4> <div class="schedule-speaker-abstract-content"><p style="text-align: justify;"> Chronic diseases confer tissue and organ damage that reduce quality of life and are widely refractory to therapy. Degenerative diseases are strongly associated with chronic inflammation in patients who are candidates for regenerative medicine treatments. Although stem cells hold promise for treating degenerative diseases, the regenerative capacity of stem cells is influenced by regulatory networks guided by local immune responses to tissue damage. Recent research has turned to how cellular and signaling components of the local stromal microenvironment (the 'soil' to the stem cells' seed), such as local inflammatory reactions, contribute to successful tissue regeneration. For this reason, it is important to consider that all measures cabale of decreasing the degree of inflammation in the body are appropriate for the preparation of the patient: sleep, gut microbiome,    environmental toxins, hormone balance, electromagnetic fields and other illness conditions, to pose the patient in a balanced position for the regenerative procedures. 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