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Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and ELISA (ELISA) applications. This antibody reacts with Bovine, Drosophila, Human, Mouse, Ovine, Plant, Rat, Xenopus laevis, Yeast, Zebrafish samples. 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as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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class="details-hero" ng-click="$ctrl.toggleImages('ADVANCED_VERIFICATION')"> <a class="link adv-verification-link animated-scroll" href="/sg/en/home/life-science/antibodies/invitrogen-antibody-validation.html"> <svg class="svg-icon advanced-verification-icon"> <use href="#icon-advanced-verification"></use> </svg> Advanced Verification </a> <p class="adv-verification-detail" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationSmLtr(event, this)"> This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. </p> </div> <div class="details-hero" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationPublishedFigures(event, this)"> <a class="link published-link animated-scroll " href="#image-gallery" onclick="window.THERMO_FISHER.pdp.inp.publishedFiguresHero(event, this)"> <svg class="svg-icon published-figures-icon"> <use href="#icon-published-figures"></use> </svg> 65 Published Figures </a> </div> <div class="details-hero"> <a class="link references-link animated-scroll " href="#references-component-id" onclick="window.THERMO_FISHER.pdp.inp.referencesHero(event, this)"> <svg class="svg-icon references-icon"> <use href="#icon-references"></use> </svg> 132 References </a> </div> <div class="details-hero"> <a class="btn hero-btn hero-view-other" href="/antibody/primary/target/actin"> View all (22) Actin antibodies </a> </div> </div> <div class="links-ab to-show-links-in-sm"> <div class="container"> <div class="link-container"> <a class="link-antibody" href="/order/genome-database/dataSheetPdf?producttype=antibody&productsubtype=antibody_primary&productId=MA1-744&version=Local" target="_blank" rel="nofollow"> <span> <svg class="svg-icon data-sheet-icon"> <use href="#icon-data-sheet"></use> </svg> </span> Datasheet </a> </div> <div class="link-container"> <a href="https://www.thermofisher.com/global/en/home/life-science/antibodies/antibodies-learning-center/antibody-protocols.html?icid=antibody-pdp-protocols" 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aria-labelledby="createCitationModal-title">  <div class="modal-content" role="document"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal">×</button> <h4 class="modal-title" id="createCitationModal-title">Cite Actin Monoclonal Antibody (mAbGEa)</h4> </div> <div class="modal-body"> <textarea id="citationTextArea" rows="5">The following product was used in this experiment: Actin Monoclonal Antibody (mAbGEa) from Thermo Fisher Scientific, catalog # MA1-744, RRID AB_2223496.</textarea> </div> <div class="modal-footer">  <button type="button" class="well-red btn btn-danger pull-left" data-dismiss="modal" onclick="copyToClipboard();" > Click to Copy </button>  </div> </div> </div> <script type="application/ld+json"> { "@context": "https://schema.org/", "@type": "BreadcrumbList", "itemListElement": [{ "@type": "ListItem", "position": 1, "name": "Home", "item":"https://www.thermofisher.com" },{ "@type": "ListItem", "position": 2, "name": "Antibodies", "item": 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Data':[{'sku':'MA1-744','imageId':'752365','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-ICC-20210323110406.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-ICC-20210323110406.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-ACTB-ICC-20210323110406.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-ACTB-ICC-20210323110406.jpg?time\u003d20240507','sortOrder':'2','description':'Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32787), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nucleus and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.','shortDescription':'Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA1744-ACTB-ICC-20210323110406.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'84140','imageUrl':'https://www.thermofisher.com/antibody/images/650/Actin-Antibody-MA1-744-IF.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/Actin-Antibody-MA1-744-IF.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/Actin-Antibody-MA1-744-IF.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/Actin-Antibody-MA1-744-IF.jpg?time\u003d20240507','sortOrder':'3','description':'Immunofluorescent analysis of Actin (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with an Actin mouse monoclonal antibody (Product # MA1-744), at a dilution of 1:100 in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.','shortDescription':'Immunofluorescent analysis of Actin (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with an Actin mouse monoclonal antibody (Product # MA1-744), at a dilution of 1:100 in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:1000 for 30 mi... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'Actin-Antibody-MA1-744-IF.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'23472','imageUrl':'https://www.thermofisher.com/antibody/images/650/Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg?time\u003d20240507','sortOrder':'4','description':'Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in Hela Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.','shortDescription':'Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in Hela Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'23471','imageUrl':'https://www.thermofisher.com/antibody/images/650/Actin_MA1-744_Immunofluorescence_A375-Cells.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/Actin_MA1-744_Immunofluorescence_A375-Cells.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/Actin_MA1-744_Immunofluorescence_A375-Cells.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/Actin_MA1-744_Immunofluorescence_A375-Cells.jpg?time\u003d20240507','sortOrder':'5','description':'Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in A375 Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.','shortDescription':'Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in A375 Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','appName':'Immunocytochemistry','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'Actin_MA1-744_Immunofluorescence_A375-Cells.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'21904','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Colon-carcinoma.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Colon-carcinoma.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1-744_Immunohistochemistry_Colon-carcinoma.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1-744_Immunohistochemistry_Colon-carcinoma.jpg?time\u003d20240507','sortOrder':'6','description':'Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.','shortDescription':'Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse mon... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'Actin Antibody (MA1-744) in IHC (P)','appAbv':'IHC (P)','appName':'Immunohistochemistry (Paraffin)','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA1-744_Immunohistochemistry_Colon-carcinoma.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'21905','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Skeletal-muscle.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Skeletal-muscle.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1-744_Immunohistochemistry_Skeletal-muscle.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1-744_Immunohistochemistry_Skeletal-muscle.jpg?time\u003d20240507','sortOrder':'7','description':'Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.','shortDescription':'Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal anti... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'Actin Antibody (MA1-744) in IHC (P)','appAbv':'IHC (P)','appName':'Immunohistochemistry (Paraffin)','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA1-744_Immunohistochemistry_Skeletal-muscle.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'21906','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1-744_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1-744_Immunohistochemistry_Tonsil-tissue.jpg?time\u003d20240507','sortOrder':'8','description':'Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.','shortDescription':'Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibo... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))','title':'Actin Antibody (MA1-744) in IHC (P)','appAbv':'IHC (P)','appName':'Immunohistochemistry (Paraffin)','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA1-744_Immunohistochemistry_Tonsil-tissue.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'752367','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-WB-20210323110406.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-WB-20210323110406.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-ACTB-WB-20210323110406.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-ACTB-WB-20210323110406.jpg?time\u003d20240507','sortOrder':'9','description':'Western Blot was performed using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) and a ~40 kDa band corresponding to Actin, cytoplasmic 1 was observed along with an uncharacterised band (*) at ~30 kDa across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), IMR-90 (Lane 4), PANC1 (Lane 5), DU 145 (Lane 6), LNCaP (Lane 7), PC-12 (Lane 8), NIH/3T3 (Lane 9), Mouse Kidney (Lane 10), Rat Kidney (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:10000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).','shortDescription':'Western Blot was performed using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) and a ~40 kDa band corresponding to Actin, cytoplasmic 1 was observed along with an uncharacterised band (*) at ~30 kDa across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), IMR-90 (Lane 4), PANC1 (Lane 5), DU 145 (Lane 6), LNCaP (Lane 7), PC-12 (Lane 8), NIH/3T3 (Lane 9), Mouse Kidney (Lane 10), Rat Kidney (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Pr... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA1744-ACTB-WB-20210323110406.jpg','benchSciPubmedId':'0'}],'Published Figures':[{'sku':'MA1-744','imageId':'1024414','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_25192_nihms-1845179-f0002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_25192_nihms-1845179-f0002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_25192_nihms-1845179-f0002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_25192_nihms-1845179-f0002.jpg?time\u003d20240507','sortOrder':'11','description':'Figure 1. Genome-wide CRISPR-Cas9 KO screens in human cells identify regulators of ataxin-2 protein levels (A) Pooled CRISPR-Cas9 screening paradigm. After transducing HeLa cells expressing Cas9 with a lentiviral whole-genome sgRNA library, we fixed and co-immunostained the cells for ataxin-2 and a control protein (beta-actin or GAPDH). We then used FACS to sort top and bottom 20% ataxin-2 expressors relative to control protein levels (duplicate sorts per each control). After isolating genomic DNA from these populations, as well as the unsorted control population, we performed NGS to read sgRNA barcodes. (B) Volcano plots based on effect and confidence scores summarizing genes that modify ataxin-2 protein levels relative to beta-actin (left) or GAPDH (right) levels when knocked out (FDR \u003c0.05). Highlighted in blue and red are hits that overlap across all screens. (C) Validation of numerous top hit genes overlapping across all screens. We transfected HeLa-Cas9 cells with non-targeting (NT) siRNAs or with siRNAs targeting mRNA transcripts encoded by hit genes, then performed immunoblotting on lysates. Quantifications are normalized to the NT siRNA condition (mean +- SD; analyzed using two-way ANOVA; ****p \u003c 0.0001, ***p \u003c 0.001, **p \u003c 0.01, *p \u003c 0.05, ns: not significant). (D) Representative immunoblot of ataxin-2 and beta-actin protein levels upon treatment with NT, PNISR , PAXBP1 , or ATXN2 siRNAs in HeLa cells.','shortDescription':'Figure 1. Genome-wide CRISPR-Cas9 KO screens in human cells identify regulators of ataxin-2 protein levels (A) Pooled CRISPR-Cas9 screening paradigm. After transducing HeLa cells expressing Cas9 with a lentiviral whole-genome sgRNA library, we fixed and co-immunostained the cells for ataxin-2 and a control protein (beta-actin or GAPDH). We then used FACS to sort top and bottom 20% ataxin-2 expressors relati... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Oct 25, 2022, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell reports','journalText':'Cell reports 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_25192_nihms-1845179-f0002.jpg','benchSciPubmedId':'36288714'},{'sku':'MA1-744','imageId':'837013','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11281_WJSC-12-203-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11281_WJSC-12-203-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11281_WJSC-12-203-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11281_WJSC-12-203-g002.jpg?time\u003d20240507','sortOrder':'12','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'Mar 26, 2020, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'World journal of stem cells','journalText':'World journal of stem cells 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11281_WJSC-12-203-g002.jpg','benchSciPubmedId':'32266052'},{'sku':'MA1-744','imageId':'331757','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-22253827-Actin-pone.0029926.g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-22253827-Actin-pone.0029926.g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-22253827-Actin-pone.0029926.g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-22253827-Actin-pone.0029926.g001.jpg?time\u003d20240507','sortOrder':'13','description':'Figure 1 ActM-GFP expression in E.coli. ActM-GFP adopts a variety of shapes and apparently is not freely diffusible in E.coli . All images show the GFP-channel, except for A2 and A3 which display the transmission channel and an overlay of transmission and GFP, respectively. Image F shows anti-actin/TRITC immunofluorescence of untagged ActM expressed in E.coli . Scale bars: 2 um.','shortDescription':'Figure 1 ActM-GFP expression in E.coli. ActM-GFP adopts a variety of shapes and apparently is not freely diffusible in E.coli . All images show the GFP-channel, except for A2 and A3 which display the transmission channel and an overlay of transmission and GFP, respectively. Image F shows anti-actin/TRITC immunofluorescence of untagged ActM expressed in E.coli . Scale bars: 2 um.','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 29, 2012, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2012 - ','sourceType':'3','benchsci':true,'imageName':'MA1744-22253827-Actin-pone.0029926.g001.jpg','benchSciPubmedId':'22253827'},{'sku':'MA1-744','imageId':'361649','imageUrl':'https://www.thermofisher.com/antibody/images/650/Actin_22253827_IF_1_pone0029926s001_20180524.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/Actin_22253827_IF_1_pone0029926s001_20180524.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/Actin_22253827_IF_1_pone0029926s001_20180524.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/Actin_22253827_IF_1_pone0029926s001_20180524.jpg?time\u003d20240507','sortOrder':'14','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'May 29, 2012, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2012 - ','sourceType':'3','benchsci':true,'imageName':'Actin_22253827_IF_1_pone0029926s001_20180524.jpg','benchSciPubmedId':'22253827'},{'sku':'MA1-744','imageId':'722908','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_1634_nihms238975f9.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_1634_nihms238975f9.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_1634_nihms238975f9.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_1634_nihms238975f9.jpg?time\u003d20240507','sortOrder':'15','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 1, 2010, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Cytoskeleton (Hoboken, N.J.)','journalText':'Cytoskeleton (Hoboken, N.J.) 2010 - ','sourceType':'3','benchsci':true,'imageName':'tfs_1634_nihms238975f9.jpg','benchSciPubmedId':'20862689'},{'sku':'MA1-744','imageId':'664353','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_56_nfig003_003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_56_nfig003_003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_56_nfig003_003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_56_nfig003_003.jpg?time\u003d20240507','sortOrder':'16','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 1, 2010, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Cytoskeleton (Hoboken, N.J.)','journalText':'Cytoskeleton (Hoboken, N.J.) 2010 - ','sourceType':'3','benchsci':true,'imageName':'tfs_56_nfig003_003.jpg','benchSciPubmedId':'20862689'},{'sku':'MA1-744','imageId':'524810','imageUrl':'https://www.thermofisher.com/antibody/images/650/nihms238975f3-1-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/nihms238975f3-1-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/nihms238975f3-1-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/nihms238975f3-1-20190221105219.jpg?time\u003d20240507','sortOrder':'17','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 1, 2010, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Cytoskeleton (Hoboken, N.J.)','journalText':'Cytoskeleton (Hoboken, N.J.) 2010 - ','sourceType':'3','benchsci':true,'imageName':'nihms238975f3-1-20190221105219.jpg','benchSciPubmedId':'20862689'},{'sku':'MA1-744','imageId':'364691','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-20862689-Actin-nihms238975f7.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-20862689-Actin-nihms238975f7.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-20862689-Actin-nihms238975f7.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-20862689-Actin-nihms238975f7.jpg?time\u003d20240507','sortOrder':'18','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 1, 2010, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Cytoskeleton (Hoboken, N.J.)','journalText':'Cytoskeleton (Hoboken, N.J.) 2010 - ','sourceType':'3','benchsci':true,'imageName':'MA1744-20862689-Actin-nihms238975f7.jpg','benchSciPubmedId':'20862689'},{'sku':'MA1-744','imageId':'364690','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-20862689-Actin-nihms238975f10.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-20862689-Actin-nihms238975f10.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-20862689-Actin-nihms238975f10.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-20862689-Actin-nihms238975f10.jpg?time\u003d20240507','sortOrder':'19','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry','altTag':'Actin Antibody in Immunocytochemistry (ICC/IF)','title':'Actin Antibody (MA1-744) in ICC/IF','appAbv':'ICC/IF','dateTime':'Nov 1, 2010, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Cytoskeleton (Hoboken, N.J.)','journalText':'Cytoskeleton (Hoboken, N.J.) 2010 - ','sourceType':'3','benchsci':true,'imageName':'MA1744-20862689-Actin-nihms238975f10.jpg','benchSciPubmedId':'20862689'},{'sku':'MA1-744','imageId':'881296','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11281_WJSC-12-203-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11281_WJSC-12-203-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11281_WJSC-12-203-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11281_WJSC-12-203-g003.jpg?time\u003d20240507','sortOrder':'20','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunohistochemistry','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunohistochemistry','altTag':'Actin Antibody in Immunohistochemistry (IHC)','title':'Actin Antibody (MA1-744) in IHC','appAbv':'IHC','dateTime':'Mar 26, 2020, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'World journal of stem cells','journalText':'World journal of stem cells 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11281_WJSC-12-203-g003.jpg','benchSciPubmedId':'32266052'},{'sku':'MA1-744','imageId':'524811','imageUrl':'https://www.thermofisher.com/antibody/images/650/molecules-23-01411-g006-1-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/molecules-23-01411-g006-1-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/molecules-23-01411-g006-1-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/molecules-23-01411-g006-1-20190221105219.jpg?time\u003d20240507','sortOrder':'21','description':'Figure 6 Expression of alpha smooth muscle actin (alpha-SMA) by immunohistochemical staining (x100). ( A ) Photomicrograph of liver section of control rats showing minimal immunostaining for alpha -SMA; ( B ) Photomicrograph of liver section of CCl 4 intoxicated rats showing extensive alpha -SMA expression of as shown by the intense brown staining; ( C ) Photomicrograph of liver section of (CCl 4 /Silymarin) treated rats showing limited alpha -SMA expression; ( D ) Photomicrograph of liver section of rats concurrently treated with CCl 4 (1 mL/kg) twice a week and ETN (100 mg/kg) three times per week, showing limited alpha -SMA expression; ( E ) Photomicrograph of liver section of rats simultaneously treated with CCl 4 (1 mL/kg) twice a week and STN (100 mg/Kg) three times per week, showing minimal alpha -SMA expression; ( F ) A graphical representation of the alpha -SMA expression as optical density (O.D) for the liver sections from different groups, where a or b express the significant difference from control or CCl 4 group respectively at p \u003c 0.05 using ANOVA followed by Tukey-Kramer as a post-hoc test.','shortDescription':'Figure 6 Expression of alpha smooth muscle actin (alpha-SMA) by immunohistochemical staining (x100). ( A ) Photomicrograph of liver section of control rats showing minimal immunostaining for alpha -SMA; ( B ) Photomicrograph of liver section of CCl 4 intoxicated rats showing extensive alpha -SMA expression of as shown by the intense brown staining; ( C ) Photomicrograph of liver section of (CCl 4 /Sily... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Immunohistochemistry (IHC)','title':'Actin Antibody (MA1-744) in IHC','appAbv':'IHC','dateTime':'Jun 11, 2018, 4:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Molecules (Basel, Switzerland)','journalText':'Molecules (Basel, Switzerland) 2018 - ','sourceType':'3','benchsci':true,'imageName':'molecules-23-01411-g006-1-20190221105219.jpg','benchSciPubmedId':'29891794'},{'sku':'MA1-744','imageId':'949497','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17817_gr1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17817_gr1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17817_gr1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17817_gr1.jpg?time\u003d20240507','sortOrder':'22','description':'Figure 1 Loss of Bpnt2 impairs glycosaminoglycan sulfation but does not alter the PAP level. A , illustration of intracellular sulfation pathways, wherein BPNT2 hydrolyzes PAP, a by-product of sulfation, to AMP. B , absent expression of Bpnt2 mRNA (*** p \u003d 0.0001) in Bpnt2-KO MEFs as determined by quantitative PCR and ( C ) absent BPNT2 protein in Bpnt2-KO MEFs, as determined by Western blot. D , Bpnt2-KO MEFs exhibit decreased levels of both intracellular ( left , **** p \u003c 0.0001) and ( E ) secreted ( right , ** p \u003d 0.0034) sulfated glycosaminoglycans, as determined by the DMMB assay. F , Bpnt2-KO MEFs do not show changes in the PAP level relative to WT cells. The bars show the mean +- SEM. Significance analyses are the results of unpaired Student\u0027s t test (two-sided). APS, adenosine phosphosulfate; BPNT1, bisphosphate nucleotidase 1; BPNT2, bisphosphate nucleotidase 2; cSULTs, cytosolic sulfotransferases; DMMB, dimethylmethylene blue; gSULTs, Golgi-resident sulfotransferases; MEFs, mouse embryonic fibroblasts; PAP, 3\u0027-phosphoadenosine-5\u0027-phosphate; PAPS, phosphoadenosine-phosphosulfate; PAPSS, PAPS synthase; PAPST, PAPS transporter.','shortDescription':'Figure 1 Loss of Bpnt2 impairs glycosaminoglycan sulfation but does not alter the PAP level. A , illustration of intracellular sulfation pathways, wherein BPNT2 hydrolyzes PAP, a by-product of sulfation, to AMP. B , absent expression of Bpnt2 mRNA (*** p \u003d 0.0001) in Bpnt2-KO MEFs as determined by quantitative PCR and ( C ) absent BPNT2 protein in Bpnt2-KO MEFs, as determined by Western blot. D , Bpnt2-KO M... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17817_gr1.jpg','benchSciPubmedId':'34634304'},{'sku':'MA1-744','imageId':'879102','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17817_gr3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17817_gr3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17817_gr3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17817_gr3.jpg?time\u003d20240507','sortOrder':'23','description':'Figure 3 Generation of mutant Bpnt2 MEF lines. A , blot for BPNT2 and actin on protein extracted from Bpnt2 MEF lines; arrows in D175N lane denote 2 bands, representing singly and doubly glycosylated protein. B , blot of proteins extracted from MEF lines, treated with PNGaseF to remove N-glycosyl groups; PNGaseF removes both glycosyl groups, resulting in a single band for BPNT2-D175N. Note that all bands shift downward with PNGaseF, as native murine BPNT2 has one N-glycosylation site at N257. C , a selection of Bpnt2 sequence from mouse and human Bpnt2 . In both, the D175N/D177N mutation results in the generation of an N-glycosyl consensus sequence. BPNT2, bisphosphate nucleotidase 2; MEF, mouse embryonic fibroblast.','shortDescription':'Figure 3 Generation of mutant Bpnt2 MEF lines. A , blot for BPNT2 and actin on protein extracted from Bpnt2 MEF lines; arrows in D175N lane denote 2 bands, representing singly and doubly glycosylated protein. B , blot of proteins extracted from MEF lines, treated with PNGaseF to remove N-glycosyl groups; PNGaseF removes both glycosyl groups, resulting in a single band for BPNT2-D175N. Note that all bands sh... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of biological chemistry','journalText':'The Journal of biological chemistry 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17817_gr3.jpg','benchSciPubmedId':'34634304'},{'sku':'MA1-744','imageId':'949252','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19905_JIR2021-6724854.006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19905_JIR2021-6724854.006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19905_JIR2021-6724854.006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19905_JIR2021-6724854.006.jpg?time\u003d20240507','sortOrder':'24','description':'Figure 6 circCOL1A/miR-145/RABL3 axis regulates the proliferation, migration, and invasion of gastric cancer. (a) Western blot results of RABL3 in AGS cells. (b) Western blot results of RABL3 in MKN-45 cells. (c) CCK8 assay was used in AGS NC and si-circCOL1A1 cells. AGS NC and si-circCOL1A1 cells were rescued by transfection of the miR-145 inhibitor or RABL3 overexpression plasmids. (d) CCK8 assay was used in the MKN-45 vector and circCOL1A1 overexpression cells. MKN-45 vector and circCOL1A1 overexpression cells were then transfected with miR-145 mimics or RABL3 siRNA. (e) Migration assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (f) Migration assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections. (g) Invasion assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (h) Invasion assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections.','shortDescription':'Figure 6 circCOL1A/miR-145/RABL3 axis regulates the proliferation, migration, and invasion of gastric cancer. (a) Western blot results of RABL3 in AGS cells. (b) Western blot results of RABL3 in MKN-45 cells. (c) CCK8 assay was used in AGS NC and si-circCOL1A1 cells. AGS NC and si-circCOL1A1 cells were rescued by transfection of the miR-145 inhibitor or RABL3 overexpression plasmids. (d) CCK8 assay was used... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Feb 7, 2022, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Journal of immunology research','journalText':'Journal of immunology research 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19905_JIR2021-6724854.006.jpg','benchSciPubmedId':'34631898'},{'sku':'MA1-744','imageId':'878773','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17679_develop-148-199477-g5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17679_develop-148-199477-g5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17679_develop-148-199477-g5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17679_develop-148-199477-g5.jpg?time\u003d20240507','sortOrder':'25','description':'Fig. 5. Y705 phosphorylation is needed for the correct localisation of STAT3 in the mitochondrion. (A) IF with anti-STAT3 and anti-ATAD3 antibodies on ESCs transiently transfected with either mStat3 , mStat3 Y705F or MLS_mStat3_NES Y705F . Arrowheads indicate the colocalisation of ATAD and STAT3. (B) Western blot of total STAT3 in ESC extracts; beta-actin was used as a loading control. (C) Western blot of mitochondrial STAT3 from ESC mitochondrial extracts; VDAC1 was used as a mitochondrial loading control, Lamin was used as a control for nuclear contamination. (D) Representative pictures of DAB IHC on ESCs acquired with TEM; positive signal is black and negative is white. Cristae are positive in Stat3 WT and MLS_Stat3_NES transfected cells. m, mitochondria; n, nucleus. Scale bar: 200 mum (A).','shortDescription':'Fig. 5. Y705 phosphorylation is needed for the correct localisation of STAT3 in the mitochondrion. (A) IF with anti-STAT3 and anti-ATAD3 antibodies on ESCs transiently transfected with either mStat3 , mStat3 Y705F or MLS_mStat3_NES Y705F . Arrowheads indicate the colocalisation of ATAD and STAT3. (B) Western blot of total STAT3 in ESC extracts; beta-actin was used as a loading control. (C) Western blot of m... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Sep 1, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Development (Cambridge, England)','journalText':'Development (Cambridge, England) 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17679_develop-148-199477-g5.jpg','benchSciPubmedId':'34473253'},{'sku':'MA1-744','imageId':'878850','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig5_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig5_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17615_41420_2021_538_Fig5_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17615_41420_2021_538_Fig5_HTML.jpg?time\u003d20240507','sortOrder':'26','description':'Fig. 5 The addition of YC-1 and 3-MA inhibited HT-induced autophagy in hypoxia and starvation tumor microenvironment, both of which involved downregulation of the HIF-1alpha/BNIP3 /Beclin1 signaling pathway. The mRNA and protein expression of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 in OSCC cells were measured by Western blot and qRT-PCR, which were normalized to beta-actin. The results were presented as the mean +- standard deviation of three independent experiments. d: HT + Hy; e: HT + Hy+3-MA; f: HT + Hy+YC-1; g: HT + Hy+YC-1 + 3-MA. HT: hyperthermia; Hy: hypoxia; YC-1: 3-(5\u0027-hydroxymethyl-2\u0027-furyl)-1-benzylindazole; 3-MA: 3-Methyladenine. * p \u003c 0.05 vs. HT + Hy group; # p \u003c 0.05 vs. HT + Hy+3-MA group; \u0026 p \u003c 0.05 vs. HT + Hy+YC-1 group; ns no significant difference ( p \u003e 0.05).','shortDescription':'Fig. 5 The addition of YC-1 and 3-MA inhibited HT-induced autophagy in hypoxia and starvation tumor microenvironment, both of which involved downregulation of the HIF-1alpha/BNIP3 /Beclin1 signaling pathway. The mRNA and protein expression of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 in OSCC cells were measured by Western blot and qRT-PCR, which were normalized to beta-actin. The results were presented as... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Aug 31, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death discovery','journalText':'Cell death discovery 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17615_41420_2021_538_Fig5_HTML.jpg','benchSciPubmedId':'34465721'},{'sku':'MA1-744','imageId':'879193','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig4_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig4_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17615_41420_2021_538_Fig4_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17615_41420_2021_538_Fig4_HTML.jpg?time\u003d20240507','sortOrder':'27','description':'Fig. 4 Hyperthermia and hypoxia-induced the expression of autophagy hallmark proteins in OSCC cells. Cal-27 and SCC-15 cells were cultured in hypoxia conditions and were exposed to 42 degC for 1 h, followed by recovery at 37 degC for 24 h. The protein and mRNA levels of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 were analyzed by qRT-PCR and western blot and normalized to those of beta-actin. Data were expressed as the mean +- SEM from three independent experiments. a: Con; b: HT; c: Hy; d: HT + Hy. Con control, HT hyperthermia, Hy hypoxia, HT + Hy hyperthermia+hypoxia. * p \u003c 0.05 vs. Con group; # p \u003c 0.05 vs. HT group; \u0026 p \u003c 0.05 vs. Hy group; no: no significant difference ( p \u003e 0.05).','shortDescription':'Fig. 4 Hyperthermia and hypoxia-induced the expression of autophagy hallmark proteins in OSCC cells. Cal-27 and SCC-15 cells were cultured in hypoxia conditions and were exposed to 42 degC for 1 h, followed by recovery at 37 degC for 24 h. The protein and mRNA levels of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 were analyzed by qRT-PCR and western blot and normalized to those of beta-actin. Data were expr... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Aug 31, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death discovery','journalText':'Cell death discovery 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17615_41420_2021_538_Fig4_HTML.jpg','benchSciPubmedId':'34465721'},{'sku':'MA1-744','imageId':'879332','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17615_41420_2021_538_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17615_41420_2021_538_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'28','description':'Fig. 3 CoCl 2 induced the mRNA and protein expression of HIF-1 alpha to construct a hypoxic microenvironment. CoCl 2 significantly induced the mRNA ( A ) and protein ( B ) and ( C ) expression of HIF-1alpha in a dose-dependent manner. Cal-27 and SCC-15 cells were co-cultured with different concentrations of CoCl2 for 24 h, then the mRNA and protein expression levels of HIF-1alpha were analyzed by qRT-PCR and Western blot, which were normalized to those of beta-actin. Data were expressed as the mean +- SEM from three independent experiments. * p \u003c 0.05, ** p \u003c 0.01, *** p \u003c 0.001 vs 50 muM CoCl 2 ; # p \u003c 0.05, ## p \u003c 0.01, ### p \u003c 0.001 vs. 50 muM CoCl 2 .','shortDescription':'Fig. 3 CoCl 2 induced the mRNA and protein expression of HIF-1 alpha to construct a hypoxic microenvironment. CoCl 2 significantly induced the mRNA ( A ) and protein ( B ) and ( C ) expression of HIF-1alpha in a dose-dependent manner. Cal-27 and SCC-15 cells were co-cultured with different concentrations of CoCl2 for 24 h, then the mRNA and protein expression levels of HIF-1alpha were analyzed by qRT-PCR an... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Aug 31, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death discovery','journalText':'Cell death discovery 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17615_41420_2021_538_Fig3_HTML.jpg','benchSciPubmedId':'34465721'},{'sku':'MA1-744','imageId':'846915','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_13993_ijms-22-03714-g010.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_13993_ijms-22-03714-g010.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_13993_ijms-22-03714-g010.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_13993_ijms-22-03714-g010.jpg?time\u003d20240507','sortOrder':'29','description':'Figure 10 Effects of a 6 h treatment with J3955 (0.75, 1.5, and 3 muM) on Cdk1 phosphorylation in HepG2 cells. After the treatment, cells were collected and the proteins were isolated for Western blot analysis as described in \u0027\u0027\u0027\u0027Materials and methods\u0027\u0027\u0027\u0027. The panel shows a representative Western blot and densitometric analysis. The values represent the ratio between phospho-Cdk1 and total Cdk1, both previously normalized for the corresponding beta-actin. Values are expressed as the mean +- S.D. of three separate experiments with similar results. Different lowercase letters on the top of each histogram indicate statistical ( p \u003c 0.05) differences among the tested samples, as measured by one-way ANOVA followed by the Tuckey test. The letter \'\'a\'\' marks the highest value. Bars not sharing the same letter were significantly different.','shortDescription':'Figure 10 Effects of a 6 h treatment with J3955 (0.75, 1.5, and 3 muM) on Cdk1 phosphorylation in HepG2 cells. After the treatment, cells were collected and the proteins were isolated for Western blot analysis as described in \u0027\u0027\u0027\u0027Materials and methods\u0027\u0027\u0027\u0027. The panel shows a representative Western blot and densitometric analysis. The values represent the ratio between phospho-Cdk1 and total Cdk1, both previo... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Apr 2, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'International journal of molecular sciences','journalText':'International journal of molecular sciences 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_13993_ijms-22-03714-g010.jpg','benchSciPubmedId':'33918281'},{'sku':'MA1-744','imageId':'844475','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_13116_nihms-1693700-f0004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_13116_nihms-1693700-f0004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_13116_nihms-1693700-f0004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_13116_nihms-1693700-f0004.jpg?time\u003d20240507','sortOrder':'30','description':'Figure 3. Stress-induced suppression of i NKT cell responses is mediated by glucocorticoids, not by SNS signals (A) WT B6 mice (n \u003d 3 or 4 per group) were injected i.p. with OHDA or Veh 6 days before they were restrained. Control cohorts were left undisturbed. Stressed and control animals were injected with alphaGC and bled at the indicated time points. Circulating IL-4 and IFN-gamma levels were measured by ELISA. (B) Protein extracts from flash-frozen spleen and brain samples from mice in (A) were loaded onto the same SDS-polyacrylamide gel and examined for their tyrosine hydroxylase (TH) content by western blotting. Staining for beta-actin was used to ensure equal protein loading. Summary data illustrate normalized TH levels after densitometry analyses. (C) Separate cohorts that had been restrained for 12 h or left undisturbed were bled for serum CS quantification by ELISA. (D and E) Mice were injected i.p. with metyrapone (D) or RU486 (E) 1 h before they were restrained for 12 h (or not) followed by an i.p. injection of alphaGC. Box-and-whisker plots show IL-4 and IFN-gamma levels at 2- and 12-h time points post-alphaGC administration, respectively, with each symbol representing an individual mouse. Statistical comparisons were made by two-way ANOVA with Dunnett\u0027\u0027s correction (A), unpaired Student\u0027\u0027s t test (C), or one-way ANOVA with Tukey\u0027\u0027s correction (D and E). *p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001, ****p \u003c 0.0001; NS, not significant.','shortDescription':'Figure 3. Stress-induced suppression of i NKT cell responses is mediated by glucocorticoids, not by SNS signals (A) WT B6 mice (n \u003d 3 or 4 per group) were injected i.p. with OHDA or Veh 6 days before they were restrained. Control cohorts were left undisturbed. Stressed and control animals were injected with alphaGC and bled at the indicated time points. Circulating IL-4 and IFN-gamma levels were measured by... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Apr 13, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell reports','journalText':'Cell reports 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_13116_nihms-1693700-f0004.jpg','benchSciPubmedId':'33852855'},{'sku':'MA1-744','imageId':'1071415','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_28300_iovs-61-12-19-f001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_28300_iovs-61-12-19-f001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_28300_iovs-61-12-19-f001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_28300_iovs-61-12-19-f001.jpg?time\u003d20240507','sortOrder':'31','description':'Figure 1. Effect of neuroretinal-specific deletion on whole retinal tissue CAV1 protein expression ( Chx10 -Cre/Cav1-flox model). ( A ) Schematic diagram illustrating the strategy for neuroretinal-specific Cav1 deletion via Chx10 -promoter driven Cre-mediated recombination of the floxed Cav1 gene. ( B ) Representative Western blots of whole cell lysates from various tissues showing retina-specific CAV1 protein reduction following Chx10 -Cre expression in the floxed Cav1 model. Protein loads ranged from 5 ug to 35 ug, depending on tissue, to detect protein within the linear range of the assay. ( C ) Histogram of Western blot protein densitometry data quantification shows retinal tissue specific CAV1 protein reduction after NR- Cav1 -depletion in Chx 10-Cre/Cav1 +/+ mice compared to Chx10 -Cre/Cav -/- controls. beta -actin was used as a loading control and for normalization. Data are mean +- SEM and were analyzed via Student\u0027s t-test (** P \u003c 0.01; N \u003d 7). ACT \u003d beta-actin.','shortDescription':'Figure 1. Effect of neuroretinal-specific deletion on whole retinal tissue CAV1 protein expression ( Chx10 -Cre/Cav1-flox model). ( A ) Schematic diagram illustrating the strategy for neuroretinal-specific Cav1 deletion via Chx10 -promoter driven Cre-mediated recombination of the floxed Cav1 gene. ( B ) Representative Western blots of whole cell lysates from various tissues showing retina-specific CAV1 prot... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Oct 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Investigative ophthalmology \u0026 visual science','journalText':'Investigative ophthalmology \u0026 visual science 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_28300_iovs-61-12-19-f001.jpg','benchSciPubmedId':'33079993'},{'sku':'MA1-744','imageId':'837942','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11877_cancers-12-02680-g007.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11877_cancers-12-02680-g007.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11877_cancers-12-02680-g007.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11877_cancers-12-02680-g007.jpg?time\u003d20240507','sortOrder':'32','description':'Figure 7 The total level of DICER, N-cadherin and E-cadherin proteins in A2780, A2780cis, SK-OV-3, and OVCAR-3 cell lines-Immunoblots. A2780, A2780cis, SK-OV-3, OVCAR3 ovarian cancer cell lines were cultured, harvested, and lysed with RIPA buffer. This Figure contains representative immunoblots of DICER, N-cadherin, E-cadherin, and beta-actin proteins level, which were obtained with the Immunoblotting-ECL method.','shortDescription':'Figure 7 The total level of DICER, N-cadherin and E-cadherin proteins in A2780, A2780cis, SK-OV-3, and OVCAR-3 cell lines-Immunoblots. A2780, A2780cis, SK-OV-3, OVCAR3 ovarian cancer cell lines were cultured, harvested, and lysed with RIPA buffer. This Figure contains representative immunoblots of DICER, N-cadherin, E-cadherin, and beta-actin proteins level, which were obtained with the Immunoblotting-ECL m... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Sep 19, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cancers','journalText':'Cancers 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11877_cancers-12-02680-g007.jpg','benchSciPubmedId':'32961797'},{'sku':'MA1-744','imageId':'948595','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19570_ol-20-03-3006-g00.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19570_ol-20-03-3006-g00.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19570_ol-20-03-3006-g00.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19570_ol-20-03-3006-g00.jpg?time\u003d20240507','sortOrder':'33','description':'Figure 1. Expression levels of IL-21 in OSCC tissues. (A) mRNA expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using reverse transcription-quantitative PCR. (B) Protein expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using western blotting. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data are presented as the mean +- SEM from three independent experiments. **P\u003c0.01 vs. the normal tissues. (D) Representative immunohistochemistry images of IL-21 expression levels in OSCC tissues and adjacent normal tissues. Magnification, x100 or x400. IL-21, interleukin-21; OSCC, oral squamous cell carcinoma; N, Normal tissues; C, cancer tissues.','shortDescription':'Figure 1. Expression levels of IL-21 in OSCC tissues. (A) mRNA expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using reverse transcription-quantitative PCR. (B) Protein expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using western blotting. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Sep 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Oncology letters','journalText':'Oncology letters 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19570_ol-20-03-3006-g00.jpg','benchSciPubmedId':'32782618'},{'sku':'MA1-744','imageId':'948596','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19570_ol-20-03-3006-g01.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19570_ol-20-03-3006-g01.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19570_ol-20-03-3006-g01.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19570_ol-20-03-3006-g01.jpg?time\u003d20240507','sortOrder':'34','description':'Figure 2. Adenoviral-mediated overexpression of IL-21 in CAL-27 cells. (A) Reverse transcription-quantitative PCR or (B) western blotting was used to analyze the mRNA or protein expression levels, respectively, of IL-21 in CAL-27 cells infected with Ad-null or Ad-IL-21. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data were expressed as the mean +- SEM from three independent experiments. *P\u003c0.05 vs. the control group; # P\u003c0.05 vs. the Ad-null group. IL-21, interleukin-21; Ad, adenovirus.','shortDescription':'Figure 2. Adenoviral-mediated overexpression of IL-21 in CAL-27 cells. (A) Reverse transcription-quantitative PCR or (B) western blotting was used to analyze the mRNA or protein expression levels, respectively, of IL-21 in CAL-27 cells infected with Ad-null or Ad-IL-21. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The dat... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Sep 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Oncology letters','journalText':'Oncology letters 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19570_ol-20-03-3006-g01.jpg','benchSciPubmedId':'32782618'},{'sku':'MA1-744','imageId':'877375','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17109_pgen.1008755.g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17109_pgen.1008755.g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17109_pgen.1008755.g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17109_pgen.1008755.g001.jpg?time\u003d20240507','sortOrder':'35','description':'10.1371/journal.pgen.1008755.g001 Fig 1 Okazaki fragments increase in length when Pol alpha is limiting. (A). Western blot against 13xMyc-tagged Pol1 from S. cerevisiae from a wild-type or GDPOL1 strain, as indicated, shifted to YPD or YEP + 3% raffinose (hereafter, media) supplemented with various concentrations of galactose. The lower band indicated by an asterisk is a degradation product resulting from degron-tagging. (B). Schematic of experimental workflow for Okazaki fragment analysis and sequencing (also see methods ). (C, D). Alkaline agarose gel analysis of end-labeled Okazaki fragments from a wild type (C) or GDPOL1 (D) strain, shifted to YPD or media supplemented with galactose as indicated. (E). Southern blot using a whole genome probe, on Okazaki fragments from a wild type or GDPOL1 strain shifted to media with the indicated sugar concentrations. (F, G). Distribution of Okazaki fragment 5\u0027 ends around consensus nucleosome dyads [] for a wild-type (F) or GDPOL1 (G) strain shifted to media containing the indicated concentration of galactose. Data for Okazaki fragment 3\u0027 ends in GDPOL1 are in S1F Fig .','shortDescription':'10.1371/journal.pgen.1008755.g001 Fig 1 Okazaki fragments increase in length when Pol alpha is limiting. (A). Western blot against 13xMyc-tagged Pol1 from S. cerevisiae from a wild-type or GDPOL1 strain, as indicated, shifted to YPD or YEP + 3% raffinose (hereafter, media) supplemented with various concentrations of galactose. The lower band indicated by an asterisk is a degradation product resulting from d... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'May 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'PLoS genetics','journalText':'PLoS genetics 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17109_pgen.1008755.g001.jpg','benchSciPubmedId':'32379761'},{'sku':'MA1-744','imageId':'837286','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11455_ddaa066f1.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11455_ddaa066f1.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11455_ddaa066f1.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11455_ddaa066f1.jpg?time\u003d20240507','sortOrder':'36','description':'Figure 1 Miro1-R272Q does not affect mitochondrial mass, but reduces mitochondrial movement. ( A ) Representative western blot images of Miro1 protein and the mitochondrial marker proteins Tom20 and Hsp60 in iPSC-derived neurons. Corresponding densitometries of western blot analyses normalized to beta-actin are shown at the right. Data indicated as mean +- SEM ( n \u003d 3). ( B ) iPSC-derived neurons were fixed and stained against Tom20. Images were obtained using a 63x objective; scale bars indicate 10 mum. ( C ) Quantification of mitochondrial area per cell from the Tom20 signal from images shown in B. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test ( n \u003d 4). ( D ) Analysis of maximum and mean speed per mitochondria from iPSC-derived neurons stained with MitoTracker Green FM. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * * * P \u003c 0.001; ( n \u003d 5).','shortDescription':'Figure 1 Miro1-R272Q does not affect mitochondrial mass, but reduces mitochondrial movement. ( A ) Representative western blot images of Miro1 protein and the mitochondrial marker proteins Tom20 and Hsp60 in iPSC-derived neurons. Corresponding densitometries of western blot analyses normalized to beta-actin are shown at the right. Data indicated as mean +- SEM ( n \u003d 3). ( B ) iPSC-derived neurons were fixed... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'May 28, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Human molecular genetics','journalText':'Human molecular genetics 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11455_ddaa066f1.jpg','benchSciPubmedId':'32280985'},{'sku':'MA1-744','imageId':'837287','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11455_ddaa066f5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11455_ddaa066f5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11455_ddaa066f5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11455_ddaa066f5.jpg?time\u003d20240507','sortOrder':'37','description':'Figure 5 Miro1-R272Q leads to alterations of autophagy flux and Rab9 protein levels. ( A ) Representative western blot image of p62 and Rab9 proteins in iPSC-derived neurons after bafilomycin A1 treatment. ( B) Densitometry of p62 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P \u003c 0.05; ( n \u003d 3). ( C ) Densitometry of Rab9 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P \u003c 0.05; ( n \u003d 3).','shortDescription':'Figure 5 Miro1-R272Q leads to alterations of autophagy flux and Rab9 protein levels. ( A ) Representative western blot image of p62 and Rab9 proteins in iPSC-derived neurons after bafilomycin A1 treatment. ( B) Densitometry of p62 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P \u003c 0.05; ( n \u003d 3). ( C ) Densitometry of Rab9 weste... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'May 28, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Human molecular genetics','journalText':'Human molecular genetics 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11455_ddaa066f5.jpg','benchSciPubmedId':'32280985'},{'sku':'MA1-744','imageId':'756014','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9601_41467_2020_14546_Fig4_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9601_41467_2020_14546_Fig4_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9601_41467_2020_14546_Fig4_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9601_41467_2020_14546_Fig4_HTML.jpg?time\u003d20240507','sortOrder':'38','description':'Fig. 4 pti-DIMs promote genome-stabilising clustering of Rad52 droplets. a - f Live-cell microscopy shows that the pti-DIM-compromising tub3Delta ( a , b ) lowers droplet velocity ( c ), increases droplet numbers ( d ) and decreases droplet size ( e ), while western blotting shows Rad53 hyper-phosphorylation ( f ). kar3 served as control. Quantifications represent the mean +- s.d. ; * P \u003c\u003d 0.0004 in chi 2 ( b , d ) and Mann-Whitney U ( c , e ) tests. n \u003d 3 independent experiments, 120 cells total ( b ). n \u003d 127 (WT) and 80 droplets ( tub3 ) over 6 independent experiments ( c ). n \u003d 11, 440 cells (WT), n \u003d 3, 120 cells each ( tub3, kar3 ) ( d ). n \u003d 123 (WT), 169 ( tub3 ), and 83 ( kar3 ) ( e ). Scale bars, 1 um. Source data are provided as a Source Data file.','shortDescription':'Fig. 4 pti-DIMs promote genome-stabilising clustering of Rad52 droplets. a - f Live-cell microscopy shows that the pti-DIM-compromising tub3Delta ( a , b ) lowers droplet velocity ( c ), increases droplet numbers ( d ) and decreases droplet size ( e ), while western blotting shows Rad53 hyper-phosphorylation ( f ). kar3 served as control. Quantifications represent the mean +- s.d. ; * P \u003c\u003d 0.0004 in chi 2 (... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Feb 4, 2020, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9601_41467_2020_14546_Fig4_HTML.jpg','benchSciPubmedId':'32019927'},{'sku':'MA1-744','imageId':'755980','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9573_elife-52063-fig5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9573_elife-52063-fig5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9573_elife-52063-fig5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9573_elife-52063-fig5.jpg?time\u003d20240507','sortOrder':'39','description':'Figure 5. Ssd1 affects mRNA localization. ( A ) Representative Western blot showing Ssd1-GFP and markers of mitochondria, ER, and vacuole as detected in organelle-depleted and organelle-enriched fractions from SSD1-GFP or untagged-Ssd1 strains (see Methods). Ssd1 fragments migrating below the expected top band emerge during the fractionation incubations. ( B ) Representative smFISH showing bud-localized M MR1 transcript in wild-type and ssd1Delta aneuploids. ( C ) Quantification of percent buds lacking MMR1 from smFISH (see Materials and methods). ( D ) Percent of cells lacking Rhodamine staining. Histograms represent average and SEM across biological triplicates; *, p\u003c0.02, Fisher\u0027s exact test.','shortDescription':'Figure 5. Ssd1 affects mRNA localization. ( A ) Representative Western blot showing Ssd1-GFP and markers of mitochondria, ER, and vacuole as detected in organelle-depleted and organelle-enriched fractions from SSD1-GFP or untagged-Ssd1 strains (see Methods). Ssd1 fragments migrating below the expected top band emerge during the fractionation incubations. ( B ) Representative smFISH showing bud-localized M M... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Jan 7, 2020, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'eLife','journalText':'eLife 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9573_elife-52063-fig5.jpg','benchSciPubmedId':'31909711'},{'sku':'MA1-744','imageId':'731330','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-6.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-6.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6332_fig-6.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6332_fig-6.jpg?time\u003d20240507','sortOrder':'40','description':'FIG. 6. LC3-dependent autophagy is affected in Miro1-mutant fibroblasts. (A) Representative Western blot image of immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 3, 6, or 24 h. (B) Densitometry analysis of Western blot for LC3-II normalized to beta-actin ( upper graph ) and the ratio of LC3-II/LC3-I protein levels ( lower graph ) from (A) . Significance calculated by the Friedman test ( n \u003d 3). (C) Immortalized fibroblasts were transfected with mito-DsRed and labeled with 18:1 NBD-PS. Then, cells were starved in medium without FBS for 2 h for subsequent live cell imaging, using a 63 x objective. Cell selection shows microscopy images with mito-DsRed-labeled mitochondria ( red ) and 18:1 NBD-PS ( green ). Cells were manually selected ( dashed pink outline ) to ensure that only 18:1 NBD-PS-labeled autophagosomes in the cytosol were analyzed. The colocalization panel shows the selected cells from the data analysis. White squares indicate the regions, which were shown in the zoom panel . Autophagosomes were identified as green particles, which are not colocalizing with the mito-DsRed signal ( white arrows ). (D) Quantification of autophagosome formation from images shown in (C) . Significance calculated by the Wilcoxon test ( n \u003d 3). (E) Colocalization of mitochondria and LC3 pixel from (F) were quantified and normalized to cell number. Significance assessed using the Mann-Whitney test ( n \u003d 3, with ~30 cells per cell line). (F) Immortalized fibroblasts were trans','shortDescription':'FIG. 6. LC3-dependent autophagy is affected in Miro1-mutant fibroblasts. (A) Representative Western blot image of immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 3, 6, or 24 h. (B) Densitometry analysis of Western blot for LC3-II normalized to beta-actin ( upper graph ) and the ratio of LC3-II/LC3-I protein levels ( lower graph ) from (A) . Significance calculated by the Friedman test ( n \u003d ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Dec 1, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Antioxidants \u0026 redox signaling','journalText':'Antioxidants \u0026 redox signaling 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6332_fig-6.jpg','benchSciPubmedId':'31303019'},{'sku':'MA1-744','imageId':'731328','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-4.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-4.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6332_fig-4.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6332_fig-4.jpg?time\u003d20240507','sortOrder':'41','description':'FIG. 4. Mutant Miro1 protein leads to reduction of mitochondrial mass. (A) Representative Western blot of Tom20 in immortalized fibroblasts. (B) Quantification of Tom20 protein level in immortalized fibroblasts from (A) . Significance tested with the Wilcoxon test ( n \u003d 20). (C) Representative Western blot image of Hsp60 protein in immortalized fibroblasts. (D) Quantification of Hsp60 normalized to beta-actin from (C) . Significance assessed using the Mann-Whitney test ( n \u003d 4). (E) Representative Western blot image of Miro1 protein in immortalized fibroblasts. (F) Quantification of Western blot analysis of Miro1 protein levels from (E) . Significance determined using the Wilcoxon test ( n \u003d 5). (G) Representative Western blot image of Tom20 and (I) MnSOD proteins in M17 cells with stable knockdown of endogenous RHOT1 and transiently overexpression of Miro1 variants. (-) Indicates M17 cells without knockdown of endogenous RHOT1 . (+) Indicates knockdown of endogenous RHOT1 by stable transfection with the RHOT1 -targeting miRNA-2471 ( RHOT1 miRNA). M17 cells were transfected with Miro1-WT/myc (in pRK5-myc vector), Miro1-WT/V5, Miro1-R272Q, or Miro1-R450C (in pcDNA3.1/V5-HisA vector). (H) Quantification of Tom20 protein levels from (G) ( n \u003d 3). (J) Quantification of MnSOD protein levels from (I) ( n \u003d 3). All data indicated as mean +- SEM. * p \u003c\u003d 0.05; ** p \u003c\u003d 0.01; *** p \u003c\u003d 0.001. miRNA, microRNA. Color images are available online.','shortDescription':'FIG. 4. Mutant Miro1 protein leads to reduction of mitochondrial mass. (A) Representative Western blot of Tom20 in immortalized fibroblasts. (B) Quantification of Tom20 protein level in immortalized fibroblasts from (A) . Significance tested with the Wilcoxon test ( n \u003d 20). (C) Representative Western blot image of Hsp60 protein in immortalized fibroblasts. (D) Quantification of Hsp60 normalized to beta-act... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Dec 1, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Antioxidants \u0026 redox signaling','journalText':'Antioxidants \u0026 redox signaling 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6332_fig-4.jpg','benchSciPubmedId':'31303019'},{'sku':'MA1-744','imageId':'731329','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6332_fig-5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6332_fig-5.jpg?time\u003d20240507','sortOrder':'42','description':'FIG. 5. Mitochondrial turnover is increased in Miro1-mutant fibroblasts. (A) Representative Western blot image of Miro1 in immortalized fibroblasts treated with 10 mu M MG132 for 24 h or with 10 n M bafilomycinA 1 for 48 h. (B) Quantification of Miro1 protein level from Western blots displayed in (A) . Significance assessed with the Wilcoxon test ( n \u003d 3-7). (C) Western blot image for Tom20 protein in immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 48 h or with 10 mu M MG132 for 24 h, respectively. (D) Quantification of Tom20 protein levels from Western blot analysis shown in (C) . Significance assessed using the Wilcoxon test ( n \u003d 5). (E) Representative Western blot image of Parkin protein. Left panel shows Parkin bands in SH-SY5Y cells overexpressing Parkin. Right panel shows endogenous Parkin in immortalized fibroblasts treated with 10 mu M FCCP for 14 h. (F) Quantification of Parkin protein levels normalized to beta-actin from (E) . Significance calculated by the Mann-Whitney test ( n \u003d 5). All data indicated as mean +- SEM. * p \u003c\u003d 0.05; ** p \u003c\u003d 0.01; *** p \u003c\u003d 0.001. FCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone. Color images are available online.','shortDescription':'FIG. 5. Mitochondrial turnover is increased in Miro1-mutant fibroblasts. (A) Representative Western blot image of Miro1 in immortalized fibroblasts treated with 10 mu M MG132 for 24 h or with 10 n M bafilomycinA 1 for 48 h. (B) Quantification of Miro1 protein level from Western blots displayed in (A) . Significance assessed with the Wilcoxon test ( n \u003d 3-7). (C) Western blot image for Tom20 protein in immor... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Dec 1, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Antioxidants \u0026 redox signaling','journalText':'Antioxidants \u0026 redox signaling 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6332_fig-5.jpg','benchSciPubmedId':'31303019'},{'sku':'MA1-744','imageId':'524812','imageUrl':'https://www.thermofisher.com/antibody/images/650/42003_2018_187_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/42003_2018_187_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/42003_2018_187_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/42003_2018_187_Fig4_HTML-1-20190221105219.jpg?time\u003d20240507','sortOrder':'43','description':'Fig. 4 Pbp1 polyQ expansions inhibit Ty1 retromobility at the post-translational level. a Effects of Pbp1-polyQ expansion on Ty1his3AI retromobility (Mean +- SD; n \u003d 5 independent cultures; Mann-Whitney U -test). b Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNA GLY loci. TEL1 amplification serves as control. c ChIP examining the localization of RNA Pol II at TyB using an alpha-RNA Pol II (phosphorylated serine 5) antibody (One-way ANOVA and Dunnett\u0027s post hoc). d Effects of Pbp1-polyQ expansion on the levels of Ty1 mRNA in RT-qPCR (One-way ANOVA and Dunnett\u0027s post hoc). e Confirmation of ATXN2-polyQ expression in transfected HEK293T cells by western blotting. f Effects of ATXN2 polyQ expansion on the levels of retroelement RNAs as detected by RT-qPCR. Values are normalized to GAPDH and statistics are relative to 23Q (Student\u0027s t -test). g Pbp1 polyQ forms exhibit an increased interaction with Trf4 ( n \u003d 2). TAP-tagged Pbp1 proteins with indicated glutamine features were subjected to anti-TAP pulldowns followed by immunoblotting for endogenous Trf4, TAP tag or Actin control. h , i Interactions between Pbp1 forms or Trf4 with Ty1 mRNA. Presented are levels of Ty1 mRNA immunoprecipitated relative to input, as detected by RT-qPCR. Values are normalized to pull-down in untagged ( h ) or trf4 cells ( i ). j Effects of Pbp1-polyQ expansion on Ty1 mRNA levels in the presence/absence of TRF4 as detected by RT-qPCR (One','shortDescription':'Fig. 4 Pbp1 polyQ expansions inhibit Ty1 retromobility at the post-translational level. a Effects of Pbp1-polyQ expansion on Ty1his3AI retromobility (Mean +- SD; n \u003d 5 independent cultures; Mann-Whitney U -test). b Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNA GLY loci. TEL1 amplification serves as control. c ChIP examining the localization of RNA Pol II at ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Jan 11, 2020, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Communications biology','journalText':'Communications biology 2020 - ','sourceType':'3','benchsci':true,'imageName':'42003_2018_187_Fig4_HTML-1-20190221105219.jpg','benchSciPubmedId':'30417124'},{'sku':'MA1-744','imageId':'487151','imageUrl':'https://www.thermofisher.com/antibody/images/650/13567_2018_565_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/13567_2018_565_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/13567_2018_565_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/13567_2018_565_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','sortOrder':'44','description':'Figure 1 Characterization of PPRV infection in goat PBMC. A Morphological changes in goat PBMC at different time points after PPRV infection (MOI \u003d 1), with mock-infected cells as a control. B Western blot analysis of N protein in PPRV-infected and mock-infected goat PBMC. Equal amounts of protein from PPRV to mock-infected cells were separated using SDS-PAGE and transferred to PVDF membranes. The membranes were probed with N antibody. beta-actin was used as the internal reference.','shortDescription':'Figure 1 Characterization of PPRV infection in goat PBMC. A Morphological changes in goat PBMC at different time points after PPRV infection (MOI \u003d 1), with mock-infected cells as a control. B Western blot analysis of N protein in PPRV-infected and mock-infected goat PBMC. Equal amounts of protein from PPRV to mock-infected cells were separated using SDS-PAGE and transferred to PVDF membranes. The membranes... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Jul 16, 2018, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Veterinary research','journalText':'Veterinary research 2018 - ','sourceType':'3','benchsci':true,'imageName':'13567_2018_565_Fig1_HTML-1-20190103084817.jpg','benchSciPubmedId':'30012212'},{'sku':'MA1-744','imageId':'755376','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870008.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870008.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8957_zh00101781870008.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8957_zh00101781870008.jpg?time\u003d20240507','sortOrder':'45','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2017, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'American journal of physiology. Cell physiology','journalText':'American journal of physiology. Cell physiology 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_8957_zh00101781870008.jpg','benchSciPubmedId':'28835433'},{'sku':'MA1-744','imageId':'755375','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870007.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870007.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8957_zh00101781870007.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8957_zh00101781870007.jpg?time\u003d20240507','sortOrder':'46','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2017, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'American journal of physiology. Cell physiology','journalText':'American journal of physiology. Cell physiology 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_8957_zh00101781870007.jpg','benchSciPubmedId':'28835433'},{'sku':'MA1-744','imageId':'755374','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8957_zh00101781870006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8957_zh00101781870006.jpg?time\u003d20240507','sortOrder':'47','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2017, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'American journal of physiology. Cell physiology','journalText':'American journal of physiology. Cell physiology 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_8957_zh00101781870006.jpg','benchSciPubmedId':'28835433'},{'sku':'MA1-744','imageId':'755373','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8957_zh00101781870005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8957_zh00101781870005.jpg?time\u003d20240507','sortOrder':'48','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2017, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'American journal of physiology. Cell physiology','journalText':'American journal of physiology. Cell physiology 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_8957_zh00101781870005.jpg','benchSciPubmedId':'28835433'},{'sku':'MA1-744','imageId':'755372','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8957_zh00101781870004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8957_zh00101781870004.jpg?time\u003d20240507','sortOrder':'49','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2017, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'American journal of physiology. Cell physiology','journalText':'American journal of physiology. Cell physiology 2017 - ','sourceType':'3','benchsci':true,'imageName':'tfs_8957_zh00101781870004.jpg','benchSciPubmedId':'28835433'},{'sku':'MA1-744','imageId':'755371','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_8957_zh00101781870003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_8957_zh00101781870003.jpg?time\u003d20240507','sortOrder':'50','description':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','shortDescription':'Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot','altTag':'Actin Antibody in Western Blot (WB)','title':'Actin Antibody (MA1-744) in WB','appAbv':'WB','dateTime':'Nov 1, 2017, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'American journal of physiology. Cell physiology','journalText':'American journal of physiology. 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Mg 2+ counters human R-loop accumulation by relying on RNaseH1 and the TRPM7 Mg 2+ transporter. ( A ) Anti-RNA-DNA hybrid immunofluorescence indicates that 10 mM Mg 2+ supplementation significantly decreases nuclear R-loop buildup in shATXN2 HeLa cells as shown in the scatter plot to better represent cell population data (N \u003d 150 per condition; Mean +- Quartiles; **** P \u003c 0.0001 for the Mann-Whitney test). ( B ) Representative anti-ATXN2 immunofluorescence images from cells transiently transfected with siCtl. or siATXN2 in the presence or absence of 10 mM Mg 2+ supplementation. Note how Mg 2+ supplementation does not alter ATXN2 levels or localization. ( C ) Anti-gammaH2AX immunofluorescence imaging and quantitation indicate that 10 mM Mg 2+ supplementation counters gammaH2AX accumulation in shATXN2 HeLa cells (N \u003d 150 per condition; Mean +- Quartiles; **** P \u003c 0.0001 for the Mann-Whitney test). ( D ) Anti-RNA-DNA hybrid immunofluorescence reveals that transient knockdown of RNaseH1, but not PIF1, abolishes the R-loop-suppressing effect of 10 mM Mg 2+ supplementation in shATXN2 HeLa cells (N \u003d 150 per condition; Mean +- Quartiles; **** P \u003c 0.0001, *** P \u003c 0.001 for the Mann-Whitney test; n.s. not statistically significant). ( E ) Immunoblots showing transient knockdown of RNaseH1 in ATXN2-deficient HeLa cells. Anti-beta-Actin immunoblotting served as loading control. ( F ) Anti-RNA-DNA hybrid immunofluorescence reveals that transient knockdown of TRPM7, but not MAGT','shortDescription':'Figure 5. Mg 2+ counters human R-loop accumulation by relying on RNaseH1 and the TRPM7 Mg 2+ transporter. 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One potential binding partner was immobilized, the other added in solution. Protein blots and immunodetection of final wash (W) and eluate (E) are shown; employed antibody is indicated at the bottom. Molecular weights are 39 kDa for ActM and 17 kDa for PfnM.','shortDescription':'Figure 4 Binding and co-elution of ActM and PfnM. One potential binding partner was immobilized, the other added in solution. Protein blots and immunodetection of final wash (W) and eluate (E) are shown; employed antibody is indicated at the bottom. 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','sourceType':'3','benchsci':true,'imageName':'MA1744-27280719-Actin-pone0157073g002.jpg','benchSciPubmedId':'27280719'}],'Advanced Verification':[{'sku':'MA1-744','imageId':'752366','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-AV-WB-20210323110406.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-AV-WB-20210323110406.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-ACTB-AV-WB-20210323110406.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-ACTB-AV-WB-20210323110406.jpg?time\u003d20240507','sortOrder':'1','description':'Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Actin, cytoplasmic 1 was observed in Mouse Spleen and Rat Spleen in comparison to Mouse Liver and Rat Liver using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) in Western Blot. doi:10.1038/bcj.2014.18 {RE}','shortDescription':'Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Actin, cytoplasmic 1 was observed in Mouse Spleen and Rat Spleen in comparison to Mouse Liver and Rat Liver using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) in Western Blot. doi:10.1038/bcj.2014.18 {RE}','altTag':'Actin Antibody','title':'Actin Antibody (MA1-744)','appAbv':'RE','appName':'Relative expression','type':'Advanced Verification','journalText':'','sourceType':'2','benchsci':false,'imageName':'MA1744-ACTB-AV-WB-20210323110406.jpg','benchSciPubmedId':'0'},{'sku':'MA1-744','imageId':'757776','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA1744-24036211-Actin-nihms524981f5.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA1744-24036211-Actin-nihms524981f5.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA1744-24036211-Actin-nihms524981f5.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA1744-24036211-Actin-nihms524981f5.jpg?time\u003d20240507','sortOrder':'10','description':'Actin associates with MTK1 and inhibition of actin polymerization with cytochalasin D inhibits MTK1/HER3 association. Comparison of mouse MTK1 and the actin interacting region (AIR) of the yeast homolog Ssk2p shows 17/40 amino acids are identical and 25/40 are conserved (Panel A). Amino acids 256-295 were deleted from the mouse MTK1 cDNA (AIR mutant). HEK-293 cells were transfected with empty vector, flag-tagged wild-type mouse MTK1 or the flag-tagged AIR mutant mouse MTK1 cDNA. After 48 h, the cells were stimulated with 20% fetal bovine serum or 0.3 M sorbitol. Flag antibody-beads were used for immunoprecipitation for flag-tagged proteins. Proteins were resolved by SDS-PAGE and immunoblotted with antibody that recognizes the FLAG epitope (B, top panel) or actin (lower panel). Cell lysates were resolved by SDS-PAGE and immunoblotted with the indicated antibodies. MCF-7 cells were pre-treated with 1 µg/ml cytochalasin D (Cyto D) for 30 min followed by 10 nM HRG for 12 min or 0.3 M sorbitol for 30 min. MTK1 was then immunoprecipitated from each condition (C, lanes a-f). Antibody directed against phosphotyrosine 1289 of HER3 was used to immunoblot (top panel). The membrane was re-probed for MTK1 (bottom panel). Cell lysates were resolved by SDS-PAGE and immunoblotted as indicated. Cytochalasin D had no effect on the phosphorylation of HER3 (lanes g \u0026 j), but diminished interaction between MTK1 and HER3 (lanes a \u0026 d). {TM}','shortDescription':'Actin associates with MTK1 and inhibition of actin polymerization with cytochalasin D inhibits MTK1/HER3 association. Comparison of mouse MTK1 and the actin interacting region (AIR) of the yeast homolog Ssk2p shows 17/40 amino acids are identical and 25/40 are conserved (Panel A). Amino acids 256-295 were deleted from the mouse MTK1 cDNA (AIR mutant). HEK-293 cells were transfected with empty vector, flag-t... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'Actin Antibody','title':'Actin Antibody (MA1-744)','appAbv':'TM','appName':'Cell treatment','type':'Advanced Verification','journalText':'','sourceType':'7','benchsci':true,'imageName':'MA1744-24036211-Actin-nihms524981f5.jpg','benchSciPubmedId':'0'}]}" image-displayed-id="'752365'" product-sub-type="'antibody_primary'" prod-type="'antibody'" page-type="PDP" product-id="'MA1-744'"> <div class="tabs"> <ul class="nav nav-pills"> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Antibody Testing Data"> <a class="tab-text"> Antibody Testing Data (8) </a> </li> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Published Figures"> <a class="tab-text"> Published Figures (65) </a> </li> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, 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<div class = "verification-certificate hide" > <span class="icon-certification"></span> <span class="adv-cert-text">{{ $ctrl.currentElement.advancedVerification.fullName }}</span> <div class="cert-provider">{{ $ctrl.currentElement.advancedVerification.text }}</div> </div> <h2 class="product-name" ng-show="!$ctrl.isVideo"> Actin Antibody (MA1-744) in ICC/IF </h2> <div ng-show="!$ctrl.isVideo"> <div class="image-desc"> <div class="product-desc-text viewing-more-long-description"> <span class="image-journal-text" > </span> <span class="short-desc"> Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... <a href="#" class="images-view-more-text" >View More</a> </span> <span class="verification-info hide" ng-if="$ctrl.hasVerificationMethod"> <a rel="nofollow" ng-href="{{$ctrl.currentElement.advancedVerification.uri}}" >{{ $ctrl.currentElement.advancedVerification.fullName }} validation info.</a> </span> <a href="#" class="images-view-more-text hide" ng-if="$ctrl.showViewMore" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)"> View more </a> </div> </div> <div class="img-desc-footer"> <div class="benchsci-pubmed-footer"> <div ng-cloak ng-if="$ctrl.currentElement.benchsci || $ctrl.sourceType === '3'" class="images-benchsci-footer"> <div class="images-benchsci-link" tabindex="0"> <span>Published figure supplied by</span> <span class="icon-benchsci-logo"> <svg id="Layer_1" data-name="Layer 1" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 89.75 23.21"><defs><style>.cls-1{fill:#222222;}</style></defs><title>benchsci-logo</title><g id="logo-blue-copy-3"><g id="logo-blue-copy-2"><path id="path14" class="cls-1" d="M34.54,11.49a2.45,2.45,0,0,0,1.59-2.34c0-1.69-1.21-2.94-3.32-2.94H28.88v10.9h4.21a3.06,3.06,0,0,0,3.37-3A2.57,2.57,0,0,0,34.54,11.49ZM31,8h1.49c1,0,1.57.53,1.57,1.36a1.36,1.36,0,0,1-1.55,1.37H31Zm1.72,7.35H31V12.47h1.75a1.42,1.42,0,0,1,1.62,1.46C34.34,14.79,33.72,15.34,32.69,15.34Z"/><path id="path16" class="cls-1" d="M41.34,9.32a3.8,3.8,0,0,0-3.72,4,3.83,3.83,0,0,0,3.91,4.05A3.39,3.39,0,0,0,45,15l-1.71-.51a1.67,1.67,0,0,1-1.72,1.15,1.85,1.85,0,0,1-1.91-1.73H45s0-.34,0-.63C45.09,10.81,43.69,9.32,41.34,9.32Zm-1.66,3.15A1.63,1.63,0,0,1,41.37,11a1.52,1.52,0,0,1,1.69,1.49Z"/><path id="path18" class="cls-1" d="M50.58,9.35a2.48,2.48,0,0,0-2.18,1.14v-1h-2v7.57h2.05V12.75a1.42,1.42,0,0,1,1.41-1.57,1.34,1.34,0,0,1,1.4,1.52v4.42h2V12.35C53.32,10.68,52.46,9.35,50.58,9.35Z"/><path id="path20" class="cls-1" d="M58.2,15.47a1.94,1.94,0,0,1-1.94-2.14,1.92,1.92,0,0,1,1.91-2.12,1.64,1.64,0,0,1,1.67,1.27l1.83-.61a3.45,3.45,0,0,0-3.55-2.55,3.83,3.83,0,0,0-2.82,1.16,3.88,3.88,0,0,0-1.1,2.85,3.9,3.9,0,0,0,4,4,3.5,3.5,0,0,0,3.53-2.55l-1.8-.6A1.72,1.72,0,0,1,58.2,15.47Z"/><path id="path22" class="cls-1" d="M67,9.35a2.72,2.72,0,0,0-2.07.83V6h-2V17.12h2V12.67a1.43,1.43,0,0,1,1.42-1.48,1.34,1.34,0,0,1,1.4,1.53v4.41h2V12.35A2.67,2.67,0,0,0,67,9.35Z"/><path id="path24" class="cls-1" d="M75.82,11.05l-1.63-.35a1.58,1.58,0,0,1-1.4-1.59A1.93,1.93,0,0,1,74.9,7.3a2.21,2.21,0,0,1,2.3,1.86l1.36-.48a3.73,3.73,0,0,0-7.29.54,3,3,0,0,0,2.67,3l1.55.33c1.09.24,1.63.91,1.63,1.7,0,.95-.74,1.74-2.25,1.74a2.51,2.51,0,0,1-1.79-.63,2.45,2.45,0,0,1-.82-1.71l-1.45.46a3.79,3.79,0,0,0,4.08,3.24c2.32,0,3.76-1.52,3.76-3.22C78.65,12.59,77.63,11.45,75.82,11.05Z"/><path id="path26" class="cls-1" d="M83.25,16A2.33,2.33,0,0,1,81,13.41a2.31,2.31,0,0,1,2.24-2.59,1.93,1.93,0,0,1,2,1.57l1.31-.55a3.22,3.22,0,0,0-3.29-2.35,3.7,3.7,0,0,0-3.72,3.92,3.75,3.75,0,0,0,3.74,3.94A3.41,3.41,0,0,0,86.59,15l-1.28-.55A2,2,0,0,1,83.25,16Z"/><path id="path28" class="cls-1" d="M88.69,5.89a1,1,0,1,0,.75.29A1,1,0,0,0,88.69,5.89Z"/><polygon id="rect30" class="cls-1" points="87.99 9.72 89.42 9.72 89.42 17.11 87.99 17.11 87.99 9.72"/><path id="path32" class="cls-1" d="M14.58,8.87A4.1,4.1,0,0,0,15.8,6H14.31a2.62,2.62,0,0,1-.76,1.87L11.66,9.7l-2-2A2.61,2.61,0,0,1,9,6H7.53A4.09,4.09,0,0,0,8.74,8.87l1.87,1.87L8.74,12.61a4.08,4.08,0,0,0-1.21,2.92v2.29H9.18V16.73h1.66v1.09h1.64V16.73h1.65v1.09H15.8V15.53a4.11,4.11,0,0,0-1.21-2.92l-1.38-1.36-1,1.05,1.37,1.36a2.63,2.63,0,0,1,.77,1.58H9a2.68,2.68,0,0,1,.76-1.58Z"/><path id="path34" class="cls-1" d="M11.61,23.21a11.61,11.61,0,1,1,11.6-11.6A11.6,11.6,0,0,1,11.61,23.21Zm0-21.89A10.29,10.29,0,1,0,21.9,11.61,10.28,10.28,0,0,0,11.61,1.32Z"/></g></g></svg> </span> <span id="bechsci-tooltip-{{$ctrl.productId}}" class="bechsci-tooltip-icon" data-toggle="tooltip" ng-click="$ctrl.showBechSciTooltip()" close-tooltip="$ctrl.closeBechSciTooltip()" data-html="true" title="Clicking this link will redirect you to the <a href='https://www.benchsci.com/' target='_blank'> BenchSci</a> website that provides third-party scientific content. 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class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="361650" data-index="68"> <img src="https://www.thermofisher.com/antibody/images/150/Actin_22253827_WB_1_pone0029926s002_20180524.jpg?time=20240507" alt="Actin Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="349924" data-index="69"> <img src="https://www.thermofisher.com/antibody/images/150/MA1744-21256200-Actin-nihms274853f1.jpg?time=20240507" alt="Actin Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="364689" data-index="70"> <img src="https://www.thermofisher.com/antibody/images/150/MA1744-19710232-Actin-pp1510590f04.jpg?time=20240507" alt="Actin Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="364688" data-index="71"> <img src="https://www.thermofisher.com/antibody/images/150/MA1744-19710232-Actin-pp1510590f03.jpg?time=20240507" alt="Actin Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="364697" data-index="72"> <img src="https://www.thermofisher.com/antibody/images/150/MA1744-27280719-Actin-pone0157073g002.jpg?time=20240507" alt="Actin Antibody in Immunoprecipitation (IP)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="752366" data-index="73"> <img src="https://www.thermofisher.com/antibody/images/150/MA1744-ACTB-AV-WB-20210323110406.jpg?time=20240507" alt="Actin Antibody"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="757776" data-index="74"> <img src="https://www.thermofisher.com/antibody/images/150/MA1744-24036211-Actin-nihms524981f5.jpg?time=20240507" alt="Actin Antibody"/> </div> </div> </div> </div> </div> <noscript> <div itemscope itemtype="http://schema.org/ImageGallery"> <h2>Product Image Gallery</h2> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-ICC-20210323110406.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32787), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nucleus and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin-Antibody-MA1-744-IF.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Actin (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with an Actin mouse monoclonal antibody (Product # MA1-744), at a dilution of 1:100 in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in Hela Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_MA1-744_Immunofluorescence_A375-Cells.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in A375 Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Colon-carcinoma.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="Actin Antibody (MA1-744) in IHC (P)"> <figcaption itemprop="caption">Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Skeletal-muscle.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="Actin Antibody (MA1-744) in IHC (P)"> <figcaption itemprop="caption">Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1-744_Immunohistochemistry_Tonsil-tissue.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))" title="Actin Antibody (MA1-744) in IHC (P)"> <figcaption itemprop="caption">Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-WB-20210323110406.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Western Blot was performed using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) and a ~40 kDa band corresponding to Actin, cytoplasmic 1 was observed along with an uncharacterised band (*) at ~30 kDa across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), IMR-90 (Lane 4), PANC1 (Lane 5), DU 145 (Lane 6), LNCaP (Lane 7), PC-12 (Lane 8), NIH/3T3 (Lane 9), Mouse Kidney (Lane 10), Rat Kidney (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:10000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_25192_nihms-1845179-f0002.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 1. Genome-wide CRISPR-Cas9 KO screens in human cells identify regulators of ataxin-2 protein levels (A) Pooled CRISPR-Cas9 screening paradigm. After transducing HeLa cells expressing Cas9 with a lentiviral whole-genome sgRNA library, we fixed and co-immunostained the cells for ataxin-2 and a control protein (beta-actin or GAPDH). We then used FACS to sort top and bottom 20% ataxin-2 expressors relative to control protein levels (duplicate sorts per each control). After isolating genomic DNA from these populations, as well as the unsorted control population, we performed NGS to read sgRNA barcodes. (B) Volcano plots based on effect and confidence scores summarizing genes that modify ataxin-2 protein levels relative to beta-actin (left) or GAPDH (right) levels when knocked out (FDR <0.05). Highlighted in blue and red are hits that overlap across all screens. (C) Validation of numerous top hit genes overlapping across all screens. We transfected HeLa-Cas9 cells with non-targeting (NT) siRNAs or with siRNAs targeting mRNA transcripts encoded by hit genes, then performed immunoblotting on lysates. Quantifications are normalized to the NT siRNA condition (mean +- SD; analyzed using two-way ANOVA; ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns: not significant). (D) Representative immunoblot of ataxin-2 and beta-actin protein levels upon treatment with NT, PNISR , PAXBP1 , or ATXN2 siRNAs in HeLa cells.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11281_WJSC-12-203-g002.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-22253827-Actin-pone.0029926.g001.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Figure 1 ActM-GFP expression in E.coli. ActM-GFP adopts a variety of shapes and apparently is not freely diffusible in E.coli . All images show the GFP-channel, except for A2 and A3 which display the transmission channel and an overlay of transmission and GFP, respectively. Image F shows anti-actin/TRITC immunofluorescence of untagged ActM expressed in E.coli . Scale bars: 2 um.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_22253827_IF_1_pone0029926s001_20180524.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_1634_nihms238975f9.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_56_nfig003_003.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/nihms238975f3-1-20190221105219.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-20862689-Actin-nihms238975f7.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-20862689-Actin-nihms238975f10.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunocytochemistry (ICC/IF)" title="Actin Antibody (MA1-744) in ICC/IF"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunocytochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11281_WJSC-12-203-g003.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunohistochemistry (IHC)" title="Actin Antibody (MA1-744) in IHC"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunohistochemistry</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/molecules-23-01411-g006-1-20190221105219.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunohistochemistry (IHC)" title="Actin Antibody (MA1-744) in IHC"> <figcaption itemprop="caption">Figure 6 Expression of alpha smooth muscle actin (alpha-SMA) by immunohistochemical staining (x100). ( A ) Photomicrograph of liver section of control rats showing minimal immunostaining for alpha -SMA; ( B ) Photomicrograph of liver section of CCl 4 intoxicated rats showing extensive alpha -SMA expression of as shown by the intense brown staining; ( C ) Photomicrograph of liver section of (CCl 4 /Silymarin) treated rats showing limited alpha -SMA expression; ( D ) Photomicrograph of liver section of rats concurrently treated with CCl 4 (1 mL/kg) twice a week and ETN (100 mg/kg) three times per week, showing limited alpha -SMA expression; ( E ) Photomicrograph of liver section of rats simultaneously treated with CCl 4 (1 mL/kg) twice a week and STN (100 mg/Kg) three times per week, showing minimal alpha -SMA expression; ( F ) A graphical representation of the alpha -SMA expression as optical density (O.D) for the liver sections from different groups, where a or b express the significant difference from control or CCl 4 group respectively at p < 0.05 using ANOVA followed by Tukey-Kramer as a post-hoc test.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17817_gr1.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 1 Loss of Bpnt2 impairs glycosaminoglycan sulfation but does not alter the PAP level. A , illustration of intracellular sulfation pathways, wherein BPNT2 hydrolyzes PAP, a by-product of sulfation, to AMP. B , absent expression of Bpnt2 mRNA (*** p = 0.0001) in Bpnt2-KO MEFs as determined by quantitative PCR and ( C ) absent BPNT2 protein in Bpnt2-KO MEFs, as determined by Western blot. D , Bpnt2-KO MEFs exhibit decreased levels of both intracellular ( left , **** p < 0.0001) and ( E ) secreted ( right , ** p = 0.0034) sulfated glycosaminoglycans, as determined by the DMMB assay. F , Bpnt2-KO MEFs do not show changes in the PAP level relative to WT cells. The bars show the mean +- SEM. Significance analyses are the results of unpaired Student's t test (two-sided). APS, adenosine phosphosulfate; BPNT1, bisphosphate nucleotidase 1; BPNT2, bisphosphate nucleotidase 2; cSULTs, cytosolic sulfotransferases; DMMB, dimethylmethylene blue; gSULTs, Golgi-resident sulfotransferases; MEFs, mouse embryonic fibroblasts; PAP, 3'-phosphoadenosine-5'-phosphate; PAPS, phosphoadenosine-phosphosulfate; PAPSS, PAPS synthase; PAPST, PAPS transporter.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17817_gr3.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 3 Generation of mutant Bpnt2 MEF lines. A , blot for BPNT2 and actin on protein extracted from Bpnt2 MEF lines; arrows in D175N lane denote 2 bands, representing singly and doubly glycosylated protein. B , blot of proteins extracted from MEF lines, treated with PNGaseF to remove N-glycosyl groups; PNGaseF removes both glycosyl groups, resulting in a single band for BPNT2-D175N. Note that all bands shift downward with PNGaseF, as native murine BPNT2 has one N-glycosylation site at N257. C , a selection of Bpnt2 sequence from mouse and human Bpnt2 . In both, the D175N/D177N mutation results in the generation of an N-glycosyl consensus sequence. BPNT2, bisphosphate nucleotidase 2; MEF, mouse embryonic fibroblast.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19905_JIR2021-6724854.006.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 6 circCOL1A/miR-145/RABL3 axis regulates the proliferation, migration, and invasion of gastric cancer. (a) Western blot results of RABL3 in AGS cells. (b) Western blot results of RABL3 in MKN-45 cells. (c) CCK8 assay was used in AGS NC and si-circCOL1A1 cells. AGS NC and si-circCOL1A1 cells were rescued by transfection of the miR-145 inhibitor or RABL3 overexpression plasmids. (d) CCK8 assay was used in the MKN-45 vector and circCOL1A1 overexpression cells. MKN-45 vector and circCOL1A1 overexpression cells were then transfected with miR-145 mimics or RABL3 siRNA. (e) Migration assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (f) Migration assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections. (g) Invasion assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (h) Invasion assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17679_develop-148-199477-g5.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Fig. 5. Y705 phosphorylation is needed for the correct localisation of STAT3 in the mitochondrion. (A) IF with anti-STAT3 and anti-ATAD3 antibodies on ESCs transiently transfected with either mStat3 , mStat3 Y705F or MLS_mStat3_NES Y705F . Arrowheads indicate the colocalisation of ATAD and STAT3. (B) Western blot of total STAT3 in ESC extracts; beta-actin was used as a loading control. (C) Western blot of mitochondrial STAT3 from ESC mitochondrial extracts; VDAC1 was used as a mitochondrial loading control, Lamin was used as a control for nuclear contamination. (D) Representative pictures of DAB IHC on ESCs acquired with TEM; positive signal is black and negative is white. Cristae are positive in Stat3 WT and MLS_Stat3_NES transfected cells. m, mitochondria; n, nucleus. Scale bar: 200 mum (A).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig5_HTML.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Fig. 5 The addition of YC-1 and 3-MA inhibited HT-induced autophagy in hypoxia and starvation tumor microenvironment, both of which involved downregulation of the HIF-1alpha/BNIP3 /Beclin1 signaling pathway. The mRNA and protein expression of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 in OSCC cells were measured by Western blot and qRT-PCR, which were normalized to beta-actin. The results were presented as the mean +- standard deviation of three independent experiments. d: HT + Hy; e: HT + Hy+3-MA; f: HT + Hy+YC-1; g: HT + Hy+YC-1 + 3-MA. HT: hyperthermia; Hy: hypoxia; YC-1: 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole; 3-MA: 3-Methyladenine. * p < 0.05 vs. HT + Hy group; # p < 0.05 vs. HT + Hy+3-MA group; & p < 0.05 vs. HT + Hy+YC-1 group; ns no significant difference ( p > 0.05).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig4_HTML.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Fig. 4 Hyperthermia and hypoxia-induced the expression of autophagy hallmark proteins in OSCC cells. Cal-27 and SCC-15 cells were cultured in hypoxia conditions and were exposed to 42 degC for 1 h, followed by recovery at 37 degC for 24 h. The protein and mRNA levels of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 were analyzed by qRT-PCR and western blot and normalized to those of beta-actin. Data were expressed as the mean +- SEM from three independent experiments. a: Con; b: HT; c: Hy; d: HT + Hy. Con control, HT hyperthermia, Hy hypoxia, HT + Hy hyperthermia+hypoxia. * p < 0.05 vs. Con group; # p < 0.05 vs. HT group; & p < 0.05 vs. Hy group; no: no significant difference ( p > 0.05).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17615_41420_2021_538_Fig3_HTML.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Fig. 3 CoCl 2 induced the mRNA and protein expression of HIF-1 alpha to construct a hypoxic microenvironment. CoCl 2 significantly induced the mRNA ( A ) and protein ( B ) and ( C ) expression of HIF-1alpha in a dose-dependent manner. Cal-27 and SCC-15 cells were co-cultured with different concentrations of CoCl2 for 24 h, then the mRNA and protein expression levels of HIF-1alpha were analyzed by qRT-PCR and Western blot, which were normalized to those of beta-actin. Data were expressed as the mean +- SEM from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 vs 50 muM CoCl 2 ; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. 50 muM CoCl 2 .</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_13993_ijms-22-03714-g010.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 10 Effects of a 6 h treatment with J3955 (0.75, 1.5, and 3 muM) on Cdk1 phosphorylation in HepG2 cells. After the treatment, cells were collected and the proteins were isolated for Western blot analysis as described in ''''Materials and methods''''. The panel shows a representative Western blot and densitometric analysis. The values represent the ratio between phospho-Cdk1 and total Cdk1, both previously normalized for the corresponding beta-actin. Values are expressed as the mean +- S.D. of three separate experiments with similar results. Different lowercase letters on the top of each histogram indicate statistical ( p < 0.05) differences among the tested samples, as measured by one-way ANOVA followed by the Tuckey test. The letter ""a"" marks the highest value. Bars not sharing the same letter were significantly different.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_13116_nihms-1693700-f0004.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 3. Stress-induced suppression of i NKT cell responses is mediated by glucocorticoids, not by SNS signals (A) WT B6 mice (n = 3 or 4 per group) were injected i.p. with OHDA or Veh 6 days before they were restrained. Control cohorts were left undisturbed. Stressed and control animals were injected with alphaGC and bled at the indicated time points. Circulating IL-4 and IFN-gamma levels were measured by ELISA. (B) Protein extracts from flash-frozen spleen and brain samples from mice in (A) were loaded onto the same SDS-polyacrylamide gel and examined for their tyrosine hydroxylase (TH) content by western blotting. Staining for beta-actin was used to ensure equal protein loading. Summary data illustrate normalized TH levels after densitometry analyses. (C) Separate cohorts that had been restrained for 12 h or left undisturbed were bled for serum CS quantification by ELISA. (D and E) Mice were injected i.p. with metyrapone (D) or RU486 (E) 1 h before they were restrained for 12 h (or not) followed by an i.p. injection of alphaGC. Box-and-whisker plots show IL-4 and IFN-gamma levels at 2- and 12-h time points post-alphaGC administration, respectively, with each symbol representing an individual mouse. Statistical comparisons were made by two-way ANOVA with Dunnett''s correction (A), unpaired Student''s t test (C), or one-way ANOVA with Tukey''s correction (D and E). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; NS, not significant.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_28300_iovs-61-12-19-f001.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 1. Effect of neuroretinal-specific deletion on whole retinal tissue CAV1 protein expression ( Chx10 -Cre/Cav1-flox model). ( A ) Schematic diagram illustrating the strategy for neuroretinal-specific Cav1 deletion via Chx10 -promoter driven Cre-mediated recombination of the floxed Cav1 gene. ( B ) Representative Western blots of whole cell lysates from various tissues showing retina-specific CAV1 protein reduction following Chx10 -Cre expression in the floxed Cav1 model. Protein loads ranged from 5 ug to 35 ug, depending on tissue, to detect protein within the linear range of the assay. ( C ) Histogram of Western blot protein densitometry data quantification shows retinal tissue specific CAV1 protein reduction after NR- Cav1 -depletion in Chx 10-Cre/Cav1 +/+ mice compared to Chx10 -Cre/Cav -/- controls. beta -actin was used as a loading control and for normalization. Data are mean +- SEM and were analyzed via Student's t-test (** P < 0.01; N = 7). ACT = beta-actin.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11877_cancers-12-02680-g007.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 7 The total level of DICER, N-cadherin and E-cadherin proteins in A2780, A2780cis, SK-OV-3, and OVCAR-3 cell lines-Immunoblots. A2780, A2780cis, SK-OV-3, OVCAR3 ovarian cancer cell lines were cultured, harvested, and lysed with RIPA buffer. This Figure contains representative immunoblots of DICER, N-cadherin, E-cadherin, and beta-actin proteins level, which were obtained with the Immunoblotting-ECL method.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19570_ol-20-03-3006-g00.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 1. Expression levels of IL-21 in OSCC tissues. (A) mRNA expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using reverse transcription-quantitative PCR. (B) Protein expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using western blotting. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data are presented as the mean +- SEM from three independent experiments. **P<0.01 vs. the normal tissues. (D) Representative immunohistochemistry images of IL-21 expression levels in OSCC tissues and adjacent normal tissues. Magnification, x100 or x400. IL-21, interleukin-21; OSCC, oral squamous cell carcinoma; N, Normal tissues; C, cancer tissues.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19570_ol-20-03-3006-g01.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 2. Adenoviral-mediated overexpression of IL-21 in CAL-27 cells. (A) Reverse transcription-quantitative PCR or (B) western blotting was used to analyze the mRNA or protein expression levels, respectively, of IL-21 in CAL-27 cells infected with Ad-null or Ad-IL-21. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data were expressed as the mean +- SEM from three independent experiments. *P<0.05 vs. the control group; # P<0.05 vs. the Ad-null group. IL-21, interleukin-21; Ad, adenovirus.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17109_pgen.1008755.g001.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">10.1371/journal.pgen.1008755.g001 Fig 1 Okazaki fragments increase in length when Pol alpha is limiting. (A). Western blot against 13xMyc-tagged Pol1 from S. cerevisiae from a wild-type or GDPOL1 strain, as indicated, shifted to YPD or YEP + 3% raffinose (hereafter, media) supplemented with various concentrations of galactose. The lower band indicated by an asterisk is a degradation product resulting from degron-tagging. (B). Schematic of experimental workflow for Okazaki fragment analysis and sequencing (also see methods ). (C, D). Alkaline agarose gel analysis of end-labeled Okazaki fragments from a wild type (C) or GDPOL1 (D) strain, shifted to YPD or media supplemented with galactose as indicated. (E). Southern blot using a whole genome probe, on Okazaki fragments from a wild type or GDPOL1 strain shifted to media with the indicated sugar concentrations. (F, G). Distribution of Okazaki fragment 5' ends around consensus nucleosome dyads [] for a wild-type (F) or GDPOL1 (G) strain shifted to media containing the indicated concentration of galactose. Data for Okazaki fragment 3' ends in GDPOL1 are in S1F Fig .</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11455_ddaa066f1.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 1 Miro1-R272Q does not affect mitochondrial mass, but reduces mitochondrial movement. ( A ) Representative western blot images of Miro1 protein and the mitochondrial marker proteins Tom20 and Hsp60 in iPSC-derived neurons. Corresponding densitometries of western blot analyses normalized to beta-actin are shown at the right. Data indicated as mean +- SEM ( n = 3). ( B ) iPSC-derived neurons were fixed and stained against Tom20. Images were obtained using a 63x objective; scale bars indicate 10 mum. ( C ) Quantification of mitochondrial area per cell from the Tom20 signal from images shown in B. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test ( n = 4). ( D ) Analysis of maximum and mean speed per mitochondria from iPSC-derived neurons stained with MitoTracker Green FM. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * * * P < 0.001; ( n = 5).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11455_ddaa066f5.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 5 Miro1-R272Q leads to alterations of autophagy flux and Rab9 protein levels. ( A ) Representative western blot image of p62 and Rab9 proteins in iPSC-derived neurons after bafilomycin A1 treatment. ( B) Densitometry of p62 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P < 0.05; ( n = 3). ( C ) Densitometry of Rab9 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P < 0.05; ( n = 3).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9601_41467_2020_14546_Fig4_HTML.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Fig. 4 pti-DIMs promote genome-stabilising clustering of Rad52 droplets. a - f Live-cell microscopy shows that the pti-DIM-compromising tub3Delta ( a , b ) lowers droplet velocity ( c ), increases droplet numbers ( d ) and decreases droplet size ( e ), while western blotting shows Rad53 hyper-phosphorylation ( f ). kar3 served as control. Quantifications represent the mean +- s.d. ; * P <= 0.0004 in chi 2 ( b , d ) and Mann-Whitney U ( c , e ) tests. n = 3 independent experiments, 120 cells total ( b ). n = 127 (WT) and 80 droplets ( tub3 ) over 6 independent experiments ( c ). n = 11, 440 cells (WT), n = 3, 120 cells each ( tub3, kar3 ) ( d ). n = 123 (WT), 169 ( tub3 ), and 83 ( kar3 ) ( e ). Scale bars, 1 um. Source data are provided as a Source Data file.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9573_elife-52063-fig5.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 5. Ssd1 affects mRNA localization. ( A ) Representative Western blot showing Ssd1-GFP and markers of mitochondria, ER, and vacuole as detected in organelle-depleted and organelle-enriched fractions from SSD1-GFP or untagged-Ssd1 strains (see Methods). Ssd1 fragments migrating below the expected top band emerge during the fractionation incubations. ( B ) Representative smFISH showing bud-localized M MR1 transcript in wild-type and ssd1Delta aneuploids. ( C ) Quantification of percent buds lacking MMR1 from smFISH (see Materials and methods). ( D ) Percent of cells lacking Rhodamine staining. Histograms represent average and SEM across biological triplicates; *, p<0.02, Fisher's exact test.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-6.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">FIG. 6. LC3-dependent autophagy is affected in Miro1-mutant fibroblasts. (A) Representative Western blot image of immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 3, 6, or 24 h. (B) Densitometry analysis of Western blot for LC3-II normalized to beta-actin ( upper graph ) and the ratio of LC3-II/LC3-I protein levels ( lower graph ) from (A) . Significance calculated by the Friedman test ( n = 3). (C) Immortalized fibroblasts were transfected with mito-DsRed and labeled with 18:1 NBD-PS. Then, cells were starved in medium without FBS for 2 h for subsequent live cell imaging, using a 63 x objective. Cell selection shows microscopy images with mito-DsRed-labeled mitochondria ( red ) and 18:1 NBD-PS ( green ). Cells were manually selected ( dashed pink outline ) to ensure that only 18:1 NBD-PS-labeled autophagosomes in the cytosol were analyzed. The colocalization panel shows the selected cells from the data analysis. White squares indicate the regions, which were shown in the zoom panel . Autophagosomes were identified as green particles, which are not colocalizing with the mito-DsRed signal ( white arrows ). (D) Quantification of autophagosome formation from images shown in (C) . Significance calculated by the Wilcoxon test ( n = 3). (E) Colocalization of mitochondria and LC3 pixel from (F) were quantified and normalized to cell number. Significance assessed using the Mann-Whitney test ( n = 3, with ~30 cells per cell line). (F) Immortalized fibroblasts were trans</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-4.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">FIG. 4. Mutant Miro1 protein leads to reduction of mitochondrial mass. (A) Representative Western blot of Tom20 in immortalized fibroblasts. (B) Quantification of Tom20 protein level in immortalized fibroblasts from (A) . Significance tested with the Wilcoxon test ( n = 20). (C) Representative Western blot image of Hsp60 protein in immortalized fibroblasts. (D) Quantification of Hsp60 normalized to beta-actin from (C) . Significance assessed using the Mann-Whitney test ( n = 4). (E) Representative Western blot image of Miro1 protein in immortalized fibroblasts. (F) Quantification of Western blot analysis of Miro1 protein levels from (E) . Significance determined using the Wilcoxon test ( n = 5). (G) Representative Western blot image of Tom20 and (I) MnSOD proteins in M17 cells with stable knockdown of endogenous RHOT1 and transiently overexpression of Miro1 variants. (-) Indicates M17 cells without knockdown of endogenous RHOT1 . (+) Indicates knockdown of endogenous RHOT1 by stable transfection with the RHOT1 -targeting miRNA-2471 ( RHOT1 miRNA). M17 cells were transfected with Miro1-WT/myc (in pRK5-myc vector), Miro1-WT/V5, Miro1-R272Q, or Miro1-R450C (in pcDNA3.1/V5-HisA vector). (H) Quantification of Tom20 protein levels from (G) ( n = 3). (J) Quantification of MnSOD protein levels from (I) ( n = 3). All data indicated as mean +- SEM. * p <= 0.05; ** p <= 0.01; *** p <= 0.001. miRNA, microRNA. Color images are available online.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6332_fig-5.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">FIG. 5. Mitochondrial turnover is increased in Miro1-mutant fibroblasts. (A) Representative Western blot image of Miro1 in immortalized fibroblasts treated with 10 mu M MG132 for 24 h or with 10 n M bafilomycinA 1 for 48 h. (B) Quantification of Miro1 protein level from Western blots displayed in (A) . Significance assessed with the Wilcoxon test ( n = 3-7). (C) Western blot image for Tom20 protein in immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 48 h or with 10 mu M MG132 for 24 h, respectively. (D) Quantification of Tom20 protein levels from Western blot analysis shown in (C) . Significance assessed using the Wilcoxon test ( n = 5). (E) Representative Western blot image of Parkin protein. Left panel shows Parkin bands in SH-SY5Y cells overexpressing Parkin. Right panel shows endogenous Parkin in immortalized fibroblasts treated with 10 mu M FCCP for 14 h. (F) Quantification of Parkin protein levels normalized to beta-actin from (E) . Significance calculated by the Mann-Whitney test ( n = 5). All data indicated as mean +- SEM. * p <= 0.05; ** p <= 0.01; *** p <= 0.001. FCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone. Color images are available online.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/42003_2018_187_Fig4_HTML-1-20190221105219.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Fig. 4 Pbp1 polyQ expansions inhibit Ty1 retromobility at the post-translational level. a Effects of Pbp1-polyQ expansion on Ty1his3AI retromobility (Mean +- SD; n = 5 independent cultures; Mann-Whitney U -test). b Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNA GLY loci. TEL1 amplification serves as control. c ChIP examining the localization of RNA Pol II at TyB using an alpha-RNA Pol II (phosphorylated serine 5) antibody (One-way ANOVA and Dunnett's post hoc). d Effects of Pbp1-polyQ expansion on the levels of Ty1 mRNA in RT-qPCR (One-way ANOVA and Dunnett's post hoc). e Confirmation of ATXN2-polyQ expression in transfected HEK293T cells by western blotting. f Effects of ATXN2 polyQ expansion on the levels of retroelement RNAs as detected by RT-qPCR. Values are normalized to GAPDH and statistics are relative to 23Q (Student's t -test). g Pbp1 polyQ forms exhibit an increased interaction with Trf4 ( n = 2). TAP-tagged Pbp1 proteins with indicated glutamine features were subjected to anti-TAP pulldowns followed by immunoblotting for endogenous Trf4, TAP tag or Actin control. h , i Interactions between Pbp1 forms or Trf4 with Ty1 mRNA. Presented are levels of Ty1 mRNA immunoprecipitated relative to input, as detected by RT-qPCR. Values are normalized to pull-down in untagged ( h ) or trf4 cells ( i ). j Effects of Pbp1-polyQ expansion on Ty1 mRNA levels in the presence/absence of TRF4 as detected by RT-qPCR (One</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/13567_2018_565_Fig1_HTML-1-20190103084817.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 1 Characterization of PPRV infection in goat PBMC. A Morphological changes in goat PBMC at different time points after PPRV infection (MOI = 1), with mock-infected cells as a control. B Western blot analysis of N protein in PPRV-infected and mock-infected goat PBMC. Equal amounts of protein from PPRV to mock-infected cells were separated using SDS-PAGE and transferred to PVDF membranes. The membranes were probed with N antibody. beta-actin was used as the internal reference.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870008.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870007.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870006.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870005.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870004.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870003.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870002.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_8957_zh00101781870001.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/yea3239-fig-0001-1-20190103084817.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_27760804_WB_1_PC_TPC201600398RAR2_f8_20180524.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-27574117-Actin-gkw752fig5.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 5. Mg 2+ counters human R-loop accumulation by relying on RNaseH1 and the TRPM7 Mg 2+ transporter. ( A ) Anti-RNA-DNA hybrid immunofluorescence indicates that 10 mM Mg 2+ supplementation significantly decreases nuclear R-loop buildup in shATXN2 HeLa cells as shown in the scatter plot to better represent cell population data (N = 150 per condition; Mean +- Quartiles; **** P < 0.0001 for the Mann-Whitney test). ( B ) Representative anti-ATXN2 immunofluorescence images from cells transiently transfected with siCtl. or siATXN2 in the presence or absence of 10 mM Mg 2+ supplementation. Note how Mg 2+ supplementation does not alter ATXN2 levels or localization. ( C ) Anti-gammaH2AX immunofluorescence imaging and quantitation indicate that 10 mM Mg 2+ supplementation counters gammaH2AX accumulation in shATXN2 HeLa cells (N = 150 per condition; Mean +- Quartiles; **** P < 0.0001 for the Mann-Whitney test). ( D ) Anti-RNA-DNA hybrid immunofluorescence reveals that transient knockdown of RNaseH1, but not PIF1, abolishes the R-loop-suppressing effect of 10 mM Mg 2+ supplementation in shATXN2 HeLa cells (N = 150 per condition; Mean +- Quartiles; **** P < 0.0001, *** P < 0.001 for the Mann-Whitney test; n.s. not statistically significant). ( E ) Immunoblots showing transient knockdown of RNaseH1 in ATXN2-deficient HeLa cells. Anti-beta-Actin immunoblotting served as loading control. ( F ) Anti-RNA-DNA hybrid immunofluorescence reveals that transient knockdown of TRPM7, but not MAGT</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-27208232-Actin-PP_PP201501741R2_f1.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tra12259-fig-0004-1-20190103084817.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tra12259-fig-0001-1-20190103084817.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-24036211-Actin-nihms524981f7.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-24036211-Actin-nihms524981f5.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-24036211-Actin-nihms524981f1.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_10643_F8.small.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-23530031-Actin-zbc0231348440008.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-23369209-Actin-1479-5876-11-29-1.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-22589468-Actin-PC_095281_wc_f7.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-22589468-Actin-PC_095281_gs_f9.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-22589468-Actin-PC_095281_gs_f6.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_22253827_WB_1_pone0029926g004_20180524.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Figure 4 Binding and co-elution of ActM and PfnM. One potential binding partner was immobilized, the other added in solution. Protein blots and immunodetection of final wash (W) and eluate (E) are shown; employed antibody is indicated at the bottom. Molecular weights are 39 kDa for ActM and 17 kDa for PfnM.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_22253827_WB_1_pone0029926s005_20180524.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_22253827_WB_1_pone0029926s003_20180524.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/Actin_22253827_WB_1_pone0029926s002_20180524.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-21256200-Actin-nihms274853f1.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-19710232-Actin-pp1510590f04.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-19710232-Actin-pp1510590f03.jpg?time=20240507?time=20240507" alt="Actin Antibody in Western Blot (WB)" title="Actin Antibody (MA1-744) in WB"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-27280719-Actin-pone0157073g002.jpg?time=20240507?time=20240507" alt="Actin Antibody in Immunoprecipitation (IP)" title="Actin Antibody (MA1-744) in IP"> <figcaption itemprop="caption">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunoprecipitation</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-ACTB-AV-WB-20210323110406.jpg?time=20240507?time=20240507" alt="Actin Antibody" title="Actin Antibody (MA1-744)"> <figcaption itemprop="caption">Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Actin, cytoplasmic 1 was observed in Mouse Spleen and Rat Spleen in comparison to Mouse Liver and Rat Liver using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) in Western Blot. doi:10.1038/bcj.2014.18 {RE}</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA1744-24036211-Actin-nihms524981f5.jpg?time=20240507?time=20240507" alt="Actin Antibody" title="Actin Antibody (MA1-744)"> <figcaption itemprop="caption">Actin associates with MTK1 and inhibition of actin polymerization with cytochalasin D inhibits MTK1/HER3 association. Comparison of mouse MTK1 and the actin interacting region (AIR) of the yeast homolog Ssk2p shows 17/40 amino acids are identical and 25/40 are conserved (Panel A). Amino acids 256-295 were deleted from the mouse MTK1 cDNA (AIR mutant). HEK-293 cells were transfected with empty vector, flag-tagged wild-type mouse MTK1 or the flag-tagged AIR mutant mouse MTK1 cDNA. After 48 h, the cells were stimulated with 20% fetal bovine serum or 0.3 M sorbitol. Flag antibody-beads were used for immunoprecipitation for flag-tagged proteins. Proteins were resolved by SDS-PAGE and immunoblotted with antibody that recognizes the FLAG epitope (B, top panel) or actin (lower panel). Cell lysates were resolved by SDS-PAGE and immunoblotted with the indicated antibodies. MCF-7 cells were pre-treated with 1 µg/ml cytochalasin D (Cyto D) for 30 min followed by 10 nM HRG for 12 min or 0.3 M sorbitol for 30 min. MTK1 was then immunoprecipitated from each condition (C, lanes a-f). Antibody directed against phosphotyrosine 1289 of HER3 was used to immunoblot (top panel). The membrane was re-probed for MTK1 (bottom panel). Cell lysates were resolved by SDS-PAGE and immunoblotted as indicated. Cytochalasin D had no effect on the phosphorylation of HER3 (lanes g & j), but diminished interaction between MTK1 and HER3 (lanes a & d). {TM}</figcaption> </figure> </div> </div> </noscript> <div id="tf-related-products-container-new"></div> <div class="product-details"> <div class="container"> <div class="row"> <div class="large-span-12 medium-span-12 small-span-12"> <h2 class="pdp-sub-heading product_details_heading">Product Details</h2></div> <div class="hidden-sku"> <h2>MA1-744</h2> </div> <div class="product-detail-section tested-app-section new-tbl-design product-detail-section table-spec"> <div class="product-container-head"> <div class="product-item"> Applications </div> <div class="product-item dilution-lbl-head "> Tested Dilution </div> <div class="product-item published-applications"> Publications </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Western Blot (WB)</h2></div> <div class="product-item "> <span class="dilution-lbl">1:1,000-1:10,000</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#WB-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 117 publications</span> <span class="mobile-view">117 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (IHC)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHC-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 4 publications</span> <span class="mobile-view">4 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (Paraffin) (IHC (P))</h2></div> <div class="product-item "> <span class="dilution-lbl">1:100</span> </div> <div class="product-item"> <span class="dilution-lbl dilution-lbl-dash">-</span> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHCP-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view"></span> <span class="mobile-view"></span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunocytochemistry (ICC/IF)</h2></div> <div class="product-item "> <span class="dilution-lbl">1:10-1:100</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ICCIF-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 4 publications</span> <span class="mobile-view">4 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Flow Cytometry (Flow)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Flow-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>ELISA (ELISA)</h2></div> <div class="product-item "> <span class="dilution-lbl">Assay-dependent</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ELISA-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunoprecipitation (IP)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IP-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>ChIP assay (ChIP)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ChIP-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Miscellaneous PubMed (Misc)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Misc-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 3 publications</span> <span class="mobile-view">3 publications</span></a> </div> </div> </div> <div class="product-detail-section tested-app-section new-tbl-design product-specific-section table-spec"> <div class="product-container-head"> <div class="product-item">Product Specifications</div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Species Reactivity</h2> </div> <div class="product-item left-flex-cls"> <span title="Bos taurus">Bovine,</span> <span title="Drosophila melanogaster">Fruit fly,</span> <span title="Homo sapiens">Human,</span> <span title="Mus musculus">Mouse,</span> <span title="Ovis aries">Sheep,</span> <span title="Embryophyta">Plant,</span> <span title="Rattus">Rat,</span> <span title="Xenopus laevis">Xenopus,</span> <span title="Yeasts">Yeast,</span> <span title="Danio rerio">Zebrafish</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Published species</h2> </div> <div class="product-item left-flex-cls"> <span>Bacteria,</span> <span>Bovine,</span> <span>Fruit fly,</span> <span>Goat,</span> <span>Human,</span> <span>Insect,</span> <span>Mouse,</span> <span>Pig,</span> <span>Plant,</span> <span>Rat,</span> <span>Xenopus,</span> <span>Yeast</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2> Host/Isotype</h2> </div> <div class="product-item left-flex-cls"> <span title="Mus musculus">Mouse / IgG1</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Class</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Monoclonal </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Type</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Antibody </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Clone</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> mAbGEa </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Immunogen </h2> </div> <div class="product-item left-flex-cls">Purified Arabidopsis actin protein. <script type="text/javascript"> if (typeof window.$mangular === 'undefined' || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = '[{"targetFamily":"Actin","uniProtId":"P68133-1","ncbiNodeId":"9606","antigenRange":"1-377","antigenLength":"377","antigenImageFileName":"MA1-744_Actin_P68133-1_house_mouse.svg","antigenImageFileNamePDP":"MA1-744_Actin_P68133-1_house_mouse_PDP.jpeg","sortOrder":"2"}]'; $mangular.isB2BCMGT = '"false"'; $mangular.isEpitopesModalImageMultiSizeEnabled = 'true'; </script> <div class="antigen-link" ng-click="$ctrl.openModal()"> <a href="">View immunogen <span class="antigen-link-icon"> <?xml version="1.0" encoding="utf-8"?>  <svg version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px" viewBox="0 0 20 20" style="enable-background:new 0 0 20 20;" xml:space="preserve"> <style type="text/css"> .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;} </style> <g id="Layer_1"> <line class="st0" x1="1.2" y1="2.5" x2="17.6" y2="2.5"/> <path d="M16.4,2.5"/> <g> <path d="M15.4,13.5"/> <path d="M15,14.9l2.8-2.8L15,9.3c0,0,0,0.9,0,1.9h-13V13h13C15.1,14,15.1,14.9,15,14.9z"/> <path d="M4.9,19.3l-2.8-2.8l2.8-2.8c0,0,0,0.9,0,1.9h13v1.8h-13C4.9,18.4,4.9,19.3,4.9,19.3z"/> </g> <g> <path d="M15.4,3.4"/> <path d="M15,6l2.8-2.8L15,0.3c0,0,0,0.9,0,1.9h-13V4h13C15.1,5.1,15.1,5.9,15,6z"/> <path d="M4.9,10.4L2.1,7.6l2.8-2.8c0,0,0,0.9,0,1.9h13v1.8h-13C4.9,9.5,4.9,10.4,4.9,10.4z"/> </g> <g> <path d="M2.8,8.1C1.3,8.1,0,6.9,0,5.4s1.3-2.7,2.8-2.7v1C1.8,3.6,1,4.4,1,5.4s0.8,1.8,1.8,1.8V8.1z"/> </g> <g> <path d="M17.2,12.6c1.5,0,2.8-1.2,2.8-2.8s-1.3-2.8-2.8-2.8v1c1,0,1.8,0.8,1.8,1.8s-0.8,1.8-1.8,1.8V12.6z"/> </g> <g> <path d="M2.8,17.1c-1.5,0-2.8-1.2-2.8-2.8s1.3-2.8,2.8-2.8v1c-1,0-1.8,0.8-1.8,1.8s0.8,1.8,1.8,1.8V17.1z"/> </g> </g> <g id="Layer_1_copy"> <path class="st1" d="M17.4,12.5c1.4-0.1,2.6-1.3,2.6-2.7c0-1.3-0.9-2.4-2.1-2.7V6.7h-13c0-1.1,0-1.9,0-1.9L2.6,7.1 C1.7,7,1,6.3,1,5.4C1,4.6,1.5,4,2.1,3.7V4h13c0,1,0,1.9,0,1.9l2.8-2.8L15,0.3c0,0,0,0.9,0,1.9h-13v0.4C0.9,3,0,4.1,0,5.4 C0,6.8,1.1,8,2.6,8.1l2.3,2.3c0,0,0-0.9,0-1.9h13V8.2C18.5,8.4,19,9.1,19,9.8c0,0.9-0.7,1.7-1.7,1.7L15,9.3c0,0,0,0.9,0,1.9h-13 v0.4C0.9,12,0,13,0,14.3c0,1.5,1.2,2.6,2.6,2.7l2.3,2.3c0,0,0-0.9,0-1.9h13v-1.8h-13c0-1.1,0-1.9,0-1.9L2.6,16 C1.7,15.9,1,15.2,1,14.3c0-0.7,0.5-1.3,1.1-1.6V13h13c0,1,0,1.9,0,1.9L17.4,12.5z"/> </g> </svg> </span> </a> </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Conjugate</h2> </div> <div class="product-item left-flex-cls"> <a ng-if="$ctrl.checkConjugate('Unconjugated ') && ''.trim() != ''" href="" ng-attr-target="{{$ctrl.getRequestCustomLinkTarget()}}" class="conjugate-value">Unconjugated </a> <span ng-if="($ctrl.checkConjugate('Unconjugated ') && ''.trim() == '')" class="conjugate-value">Unconjugated </span> <span ng-if="!$ctrl.checkConjugate('Unconjugated ')" class="conjugate-value">Unconjugated </span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Form</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Liquid </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Concentration</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Conc. Not Determined </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage buffer</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> ascites </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Contains</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.05% sodium azide </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> -20° C, Avoid Freeze/Thaw Cycles </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Shipping conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Wet ice </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"><h2>RRID <span id="rrid-tooltip" class="rrid-tooltip-icon" ng-include="'/order/genome-database/webapp/assets/core/img/svg/mono_tooltip.svg'" data-toggle="tooltip" ng-click="$ctrl.showRridTooltip()" close-tooltip="$ctrl.closeRridTooltip()" title="Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources in the biomedical literature to improve transparency of research methods."> </span></h2> </div> <div class="product-item left-flex-cls">AB_2223496</div> </div> </div> <div class="product-specific-details"> <div class="product-specific-details-tbl product-specific-add-border" > <div class="product-info-para"> <h4 class="product-info-para-heading"> Product Specific Information </h4> <p>MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.<br /><br />MA1-744 has successfully been used in Western blot and ELISA procedures. By Western blot, this antibody detects a 45 kDa protein representing actin.<br /><br /> The MA1-744 immunogen is purified actin protein from Arabidopsis.</p> </div> <div class="product-info-para"> <h4 class="product-info-para-heading"> Target Information </h4> <p class="product-specific-target-info">Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.</p> </div> <p class="product-details-regulatoryStmt">For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.</p> </div> <div class="product-detail-panel-builder"> <tf-promo-ad ng-if="$ctrl.showPromoAd" show-promo-ad="$ctrl.showPromoAd"></tf-promo-ad> </div> </div> </div> </div> </div> <div id="rootPdpDocLoader"></div> <div id="tf-related-products-container" ng-show="emptyRelatedProductsWidget === false" > <related-products-container ng-if="showRelatedProductsWidget === true" base-url="'/api/magellan/related-products-ui/widget/v2/'" product-type="'antibody'" product-sub-type="'primary'" product-id="'MA1744'" country-code="'sg'" iso-language="'en'" > </related-products-container> </div> <div class="tf-pdp-divider" ng-if="emptyRelatedProductsWidget === false" ></div> <div class="references reference-section-3" id="references-component-id"> <div id="references-section-id"> <reference-section ng-if="loadReferences" default-display-card-limit="3" reference-count="132" sub-type="'Antibody'" application-data='{"citationApplications":[{"applicationAbbreviation":"WB","citations":[{"title":"Natural Variation in the Multidrug Efflux Pump \u003ci\u003eSGE1\u003c/i\u003e Underlies Ionic Liquid Tolerance in Yeast.","summary":"MA1-744 was used in Western Blotting to identify SGE1 and a previously uncharacterised gene names ILT1 which encodes a predicted membrane protein.","authors":"Higgins DA,Young MKM,Tremaine M,Sardi M,Fletcher JM,Agnew M,Liu L,Dickinson Q,Peris D,Wrobel RL,Hittinger CT,Gasch AP,Singer SW,Simmons BA,Landick R,Thelen MP,Sato TK","literatureId":"30045857","journal":"Genetics","volume":"210","pageNumber":"219","publishedDate":"Sep 1, 2018, 4:00:00 AM","url":"http://dx.doi.org/10.1534/genetics.118.301161","benchSci":false,"imageURL":"https://www.thermofisher.com/antibody/images/250/null?time\u003d20240507","journalAbbr":"Genetics","sortOrder":41,"species":[],"speciesDilutionMap":{"Not Applicable":"1:1000"},"abbreviationToNameMap":{"WB":"Western 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itemtype="https://schema.org/ScholarlyArticle"> <h2>Product References</h2> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36650519"> <div itemprop="name">Circular RNA hsa_circ_0075323 promotes glioblastoma cells proliferation and invasion via regulation of autophagy.</div> </a> <div itemprop="about">Cell division</div> <div itemprop="author">Zhang W,Shi Z,Chen S,Shen S,Tu S,Yang J,Qiu Y,Lin Y,Dai X</div> <div itemprop="description">MA1-744 was used in Western Blotting to explore the biological function of hsa_circ_0075323 in GBM and its relationship with autophagy regulation.</div> <div itemprodp="datePublished">Tue Jan 17 00:00:00 EST 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36922596"> <div itemprop="name">Fast and sensitive GCaMP calcium indicators for imaging neural populations.</div> </a> <div itemprop="about">Nature</div> <div itemprop="author">Zhang Y,Rózsa M,Liang Y,Bushey D,Wei Z,Zheng J,Reep D,Broussard GJ,Tsang A,Tsegaye G,Narayan S,Obara CJ,Lim JX,Patel R,Zhang R,Ahrens MB,Turner GC,Wang SS,Korff WL,Schreiter ER,Svoboda K,Hasseman JP,Kolb I,Looger LL</div> <div itemprop="description">MA1-744 was used in Western Blotting to show that jGCaMP8 sensors will allow tracking of large populations of neurons on timescales relevant to neural computation.</div> <div itemprodp="datePublished">Wed Mar 01 00:00:00 EST 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36902295"> <div itemprop="name">Tryptophan Hydroxylase-2-Mediated Serotonin Biosynthesis Suppresses Cell Reprogramming into Pluripotent State.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Sinenko SA,Kuzmin AA,Skvortsova EV,Ponomartsev SV,Efimova EV,Bader M,Alenina N,Tomilin AN</div> <div itemprop="description">MA1-744 was used in Western Blotting to suggest a negative role of serotonin biosynthesis in the reprogramming of somatic cells to a pluripotent state.</div> <div itemprodp="datePublished">Thu Mar 02 00:00:00 EST 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37152643"> <div itemprop="name">Validation of CRISPR activation system in <i>Aedes</i> cells using multicistronic plasmid vectors.</div> </a> <div itemprop="about">Frontiers in bioengineering and biotechnology</div> <div itemprop="author">Jaiswal V,Varghese SA,Ghosh S</div> <div itemprop="description">MA1-744 was used in Western Blot to report implementation of the CRISPR activation system in Aedes cells.</div> <div itemprodp="datePublished">Tue May 09 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36288714"> <div itemprop="name">Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2.</div> </a> <div itemprop="about">Cell reports</div> <div itemprop="author">Kim G,Nakayama L,Blum JA,Akiyama T,Boeynaems S,Chakraborty M,Couthouis J,Tassoni-Tsuchida E,Rodriguez CM,Bassik MC,Gitler AD</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue Oct 25 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36083288"> <div itemprop="name">Astrocytes show increased levels of Ero1α in multiple sclerosis and its experimental autoimmune encephalomyelitis animal model.</div> </a> <div itemprop="about">The European journal of neuroscience</div> <div itemprop="author">Samtleben S,Mina L,Yap MC,Branton WG,Yousuf MS,Tenorio G,Ballanyi K,Giuliani F,Kerr BJ,Power C,Simmen T</div> <div itemprop="description">MA1-744 was used in Flow Cytometry, ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot to show that Ero1α induction makes astrocytes vulnerable to oxidative stress in the MS and EAE pathologies.</div> <div itemprodp="datePublished">Sat Oct 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36083288"> <div itemprop="name">Astrocytes show increased levels of Ero1α in multiple sclerosis and its experimental autoimmune encephalomyelitis animal model.</div> </a> <div itemprop="about">The European journal of neuroscience</div> <div itemprop="author">Samtleben S,Mina L,Yap MC,Branton WG,Yousuf MS,Tenorio G,Ballanyi K,Giuliani F,Kerr BJ,Power C,Simmen T</div> <div itemprop="description">MA1-744 was used in Flow Cytometry, ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot to show that Ero1α induction makes astrocytes vulnerable to oxidative stress in the MS and EAE pathologies.</div> <div itemprodp="datePublished">Sat Oct 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35432321"> <div itemprop="name">Expression of Constitutive Fusion of Ubiquitin to PCNA Restores the Level of Immunoglobulin A/T Mutations During Somatic Hypermutation in the Ramos Cell Line.</div> </a> <div itemprop="about">Frontiers in immunology</div> <div itemprop="author">Lerner LK,Bonte D,Le Guillou M,Mohammad MM,Kasraian Z,Sarasin A,Despras E,Aoufouchi S</div> <div itemprop="description">MA1-744 was used in Western Blotting to report the establishment of the first modified human B cell line that recapitulates the mechanism of SHM of Ig genes in vitro.</div> <div itemprodp="datePublished">Tue Apr 19 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36551679"> <div itemprop="name">Characterization of Glioblastoma Cells Response to Regorafenib.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Mongiardi MP,Buccarelli M,Formato A,Orecchini E,Salbini M,Ricci V,Orsini T,Putti S,Chiesa S,Ricci-Vitiani L,D'Alessandris QG,Pallini R,Levi A,Falchetti ML</div> <div itemprop="description">MA1-744 was used in Western Blotting to suggest that regorafenib limits cell growth, however, it might induce an invasive phenotype.</div> <div itemprodp="datePublished">Thu Dec 15 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35067715"> <div itemprop="name">Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes.</div> </a> <div itemprop="about">Biology open</div> <div itemprop="author">Hindul NL,Jhita A,Oprea DG,Hussain TA,Gonchar O,Campillo MAM,O'Regan L,Kanemaki MT,Fry AM,Hirota K,Tanaka K</div> <div itemprop="description">MA1-744 was used in Western Blotting to modify the AAVS1 locus to harbour a DNA fragment encoding ERT2-Cre-ERT2 fusion protein under regulation of a Tet-On expression system.</div> <div itemprodp="datePublished">Tue Feb 15 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33996257"> <div itemprop="name">Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.</div> </a> <div itemprop="about">Molecular therapy. Nucleic acids</div> <div itemprop="author">Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z</div> <div itemprop="description">MA1-744 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.</div> <div itemprodp="datePublished">Fri Jun 04 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33359909"> <div itemprop="name">Divergent erythroid megakaryocyte fates in Blvrb-deficient mice establish non-overlapping cytoprotective functions during stress hematopoiesis.</div> </a> <div itemprop="about">Free radical biology & medicine</div> <div itemprop="author">Nesbitt NM,Malone LE,Liu Z,Jares A,Gnatenko DV,Ma Y,Zhu W,Bahou WF</div> <div itemprop="description">MA1-744 was used in Western Blot to provide the first physiological function of murine Blvrb in a non-redundant pathway of stress cytoprotection.</div> <div itemprodp="datePublished">Sat Feb 20 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34473253"> <div itemprop="name">Y705 and S727 are required for the mitochondrial import and transcriptional activities of STAT3, and for regulation of stem cell proliferation.</div> </a> <div itemprop="about">Development (Cambridge, England)</div> <div itemprop="author">Peron M,Dinarello A,Meneghetti G,Martorano L,Betto RM,Facchinello N,Tesoriere A,Tiso N,Martello G,Argenton F</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Wed Sep 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34631898"> <div itemprop="name">circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression.</div> </a> <div itemprop="about">Journal of immunology research</div> <div itemprop="author">Ma Y,Ren Y,Wen H,Cui C</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Mon Feb 07 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34631898"> <div itemprop="name">circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression.</div> </a> <div itemprop="about">Journal of immunology research</div> <div itemprop="author">Ma Y,Ren Y,Wen H,Cui C</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Mon Feb 07 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34165160"> <div itemprop="name">miR‑205‑3p promotes lung cancer progression by targeting APBB2.</div> </a> <div itemprop="about">Molecular medicine reports</div> <div itemprop="author">Xu LB,Xiong J,Zhang YH,Dai Y,Ren XP,Ren YJ,Han D,Wei SH,Qi M</div> <div itemprop="description">MA1-744 was used in Western Blot to investigate the potential promotive impact of miR‚205‚3p on non‚small cell lung cancer processes and if theoretical evidence for miR‚205‚3p as a potential clinical gene therapy target exists.</div> <div itemprodp="datePublished">Sun Aug 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33884032"> <div itemprop="name">Interferon-τ regulates the expression and function of bovine leukocyte antigen by downregulating bta-miR-204.</div> </a> <div itemprop="about">Experimental and therapeutic medicine</div> <div itemprop="author">Wang X,Yuan T,Yin N,Ma X,Yang Y,Yang J,Shaukat A,Deng G</div> <div itemprop="description">MA1-744 was used in Western Blot to verify that bta-miR-204 was downregulated and that BoLA was upregulated in the uterine tissues of dairy cows during early pregnancy.</div> <div itemprodp="datePublished">Tue Jun 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33852855"> <div itemprop="name">Chronic stress physically spares but functionally impairs innate-like invariant T cells.</div> </a> <div itemprop="about">Cell reports</div> <div itemprop="author">Rudak PT,Choi J,Parkins KM,Summers KL,Jackson DN,Foster PJ,Skaro AI,Leslie K,McAlister VC,Kuchroo VK,Inoue W,Lantz O,Haeryfar SMM</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue Apr 13 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33918281"> <div itemprop="name">In Silico Identification of Small Molecules as New Cdc25 Inhibitors through the Correlation between Chemosensitivity and Protein Expression Pattern.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Lauria A,Martorana A,La Monica G,Mannino S,Mannino G,Peri D,Gentile C</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Fri Apr 02 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34465721"> <div itemprop="name">Combined effects of hyperthermia and chemotherapy on the regulate autophagy of oral squamous cell carcinoma cells under a hypoxic microenvironment.</div> </a> <div itemprop="about">Cell death discovery</div> <div itemprop="author">Shi F,Luo D,Zhou X,Sun Q,Shen P,Wang S</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue Aug 31 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34634304"> <div itemprop="name">Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Eisele BS,Luka Z,Wu AJ,Yang F,Hale AT,York JD</div> <div itemprop="description">MA1-744 was used in Western Blot to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro by using mouse embryonic fibroblasts.</div> <div itemprodp="datePublished">Mon Nov 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34634304"> <div itemprop="name">Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Eisele BS,Luka Z,Wu AJ,Yang F,Hale AT,York JD</div> <div itemprop="description">MA1-744 was used in Western Blot to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro by using mouse embryonic fibroblasts.</div> <div itemprodp="datePublished">Mon Nov 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34638677"> <div itemprop="name">Two Novel C-Terminus RUNX2 Mutations in Two Cleidocranial Dysplasia (CCD) Patients Impairing p53 Expression.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Dalle Carbonare L,Antoniazzi F,Gandini A,Orsi S,Bertacco J,Li Vigni V,Minoia A,Griggio F,Perduca M,Mottes M,Valenti MT</div> <div itemprop="description">MA1-744 was used in Western Blotting to provide new insights into the role of RUNX2 mutations in CCD patients and suggest that an in-depth analysis of the RUNX2-associated gene network may contribute to better understand the complex molecular and phenotypic alterations in mutant subjects.</div> <div itemprodp="datePublished">Sat Sep 25 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34114203"> <div itemprop="name">Antioxidant effects on hypoxia-induced oxidative stress and apoptosis in rat rotator cuff fibroblasts.</div> </a> <div itemprop="about">European cells & materials</div> <div itemprop="author">Kim RJ,An SH,Gwark JY,Park HB</div> <div itemprop="description">MA1-744 was used in Western Blot to study whether the antioxidant N-acetylcysteine (NAC) exerts cytoprotective effects against hypoxia-induced rotator cuff fibroblast apoptosis.</div> <div itemprodp="datePublished">Fri Jun 11 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34916944"> <div itemprop="name">Synergetic Effect of 4-Phenylbutyric Acid in Combination with Cyclosporine A on Cardiovascular Function in Sepsis Rats via Inhibition of Endoplasmic Reticulum Stress and Mitochondrial Permeability Transition Pore Opening.</div> </a> <div itemprop="about">Frontiers in pharmacology</div> <div itemprop="author">Kuang L,Zhu Y,Wu Y,Peng X,Tian K,Liu L,Li T</div> <div itemprop="description">MA1-744 was used in ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot to show that the combined application of PBA and CsA could significantly enhance the beneficial effects, compared with using PBA or CsA alone.</div> <div itemprodp="datePublished">Fri Feb 04 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32266052"> <div itemprop="name">Efficient differentiation of vascular smooth muscle cells from Wharton's Jelly mesenchymal stromal cells using human platelet lysate: A potential cell source for small blood vessel engineering.</div> </a> <div itemprop="about">World journal of stem cells</div> <div itemprop="author">Mallis P,Papapanagiotou A,Katsimpoulas M,Kostakis A,Siasos G,Kassi E,Stavropoulos-Giokas C,Michalopoulos E</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunohistochemistry</div> <div itemprodp="datePublished">Thu Mar 26 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32266052"> <div itemprop="name">Efficient differentiation of vascular smooth muscle cells from Wharton's Jelly mesenchymal stromal cells using human platelet lysate: A potential cell source for small blood vessel engineering.</div> </a> <div itemprop="about">World journal of stem cells</div> <div itemprop="author">Mallis P,Papapanagiotou A,Katsimpoulas M,Kostakis A,Siasos G,Kassi E,Stavropoulos-Giokas C,Michalopoulos E</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunohistochemistry</div> <div itemprodp="datePublished">Thu Mar 26 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32280985"> <div itemprop="name">Impaired mitochondrial-endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson's disease.</div> </a> <div itemprop="about">Human molecular genetics</div> <div itemprop="author">Berenguer-Escuder C,Grossmann D,Antony P,Arena G,Wasner K,Massart F,Jarazo J,Walter J,Schwamborn JC,Grünewald A,Krüger R</div> <div itemprop="description">MA1-744 was used in Western Blotting to study mitophagy and the organization and function of MERCs.</div> <div itemprodp="datePublished">Thu May 28 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32280985"> <div itemprop="name">Impaired mitochondrial-endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson's disease.</div> </a> <div itemprop="about">Human molecular genetics</div> <div itemprop="author">Berenguer-Escuder C,Grossmann D,Antony P,Arena G,Wasner K,Massart F,Jarazo J,Walter J,Schwamborn JC,Grünewald A,Krüger R</div> <div itemprop="description">MA1-744 was used in Western Blotting to study mitophagy and the organization and function of MERCs.</div> <div itemprodp="datePublished">Thu May 28 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33079993"> <div itemprop="name">Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Gurley JM,Gmyrek GB,McClellan ME,Hargis EA,Hauck SM,Dozmorov MG,Wren JD,Carr DJJ,Elliott MH</div> <div itemprop="description">MA1-744 was used in Western Blotting to use Cre/lox technology to deplete Cav1 specifically in the neural retinal (NR) compartment to clarify the role NR-specific Cav1 (NR-Cav1) in the retinal immune response to intravitreal inflammatory challenge induced by activation of Toll-like receptor-4 (TLR4).</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33079993"> <div itemprop="name">Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Gurley JM,Gmyrek GB,McClellan ME,Hargis EA,Hauck SM,Dozmorov MG,Wren JD,Carr DJJ,Elliott MH</div> <div itemprop="description">MA1-744 was used in Western Blotting to use Cre/lox technology to deplete Cav1 specifically in the neural retinal (NR) compartment to clarify the role NR-specific Cav1 (NR-Cav1) in the retinal immune response to intravitreal inflammatory challenge induced by activation of Toll-like receptor-4 (TLR4).</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32782618"> <div itemprop="name">Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma <i>in vitro</i>.</div> </a> <div itemprop="about">Oncology letters</div> <div itemprop="author">Liu H,Liu P,Sun D,Xing D,Wang X,Yang J,Wang S</div> <div itemprop="description">MA1-744 was used in Western Blotting to determine the role of IL-21 in oral squamous cell carcinoma (OSCC) in vitro.</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32782618"> <div itemprop="name">Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma <i>in vitro</i>.</div> </a> <div itemprop="about">Oncology letters</div> <div itemprop="author">Liu H,Liu P,Sun D,Xing D,Wang X,Yang J,Wang S</div> <div itemprop="description">MA1-744 was used in Western Blotting to determine the role of IL-21 in oral squamous cell carcinoma (OSCC) in vitro.</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32019927"> <div itemprop="name">DNA repair by Rad52 liquid droplets.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Oshidari R,Huang R,Medghalchi M,Tse EYW,Ashgriz N,Lee HO,Wyatt H,Mekhail K</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue Feb 04 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32379761"> <div itemprop="name">Separable, Ctf4-mediated recruitment of DNA Polymerase α for initiation of DNA synthesis at replication origins and lagging-strand priming during replication elongation.</div> </a> <div itemprop="about">PLoS genetics</div> <div itemprop="author">Porcella SY,Koussa NC,Tang CP,Kramer DN,Srivastava P,Smith DJ</div> <div itemprop="description">MA1-744 was used in Western Blotting to study Okazaki fragment initiation and ongoing lagging-strand synthesis in vivo.</div> <div itemprodp="datePublished">Fri May 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32379761"> <div itemprop="name">Separable, Ctf4-mediated recruitment of DNA Polymerase α for initiation of DNA synthesis at replication origins and lagging-strand priming during replication elongation.</div> </a> <div itemprop="about">PLoS genetics</div> <div itemprop="author">Porcella SY,Koussa NC,Tang CP,Kramer DN,Srivastava P,Smith DJ</div> <div itemprop="description">MA1-744 was used in Western Blotting to study Okazaki fragment initiation and ongoing lagging-strand synthesis in vivo.</div> <div itemprodp="datePublished">Fri May 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32961797"> <div itemprop="name">MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Wilczynski M,Kielbik M,Senderowska D,Krawczyk T,Szymanska B,Klink M,Bieńkiewicz J,Romanowicz H,Frühauf F,Malinowski A</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Sat Sep 19 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32961797"> <div itemprop="name">MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Wilczynski M,Kielbik M,Senderowska D,Krawczyk T,Szymanska B,Klink M,Bieńkiewicz J,Romanowicz H,Frühauf F,Malinowski A</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Sat Sep 19 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31909711"> <div itemprop="name">The genetic basis of aneuploidy tolerance in wild yeast.</div> </a> <div itemprop="about">eLife</div> <div itemprop="author">Hose J,Escalante LE,Clowers KJ,Dutcher HA,Robinson D,Bouriakov V,Coon JJ,Shishkova E,Gasch AP</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue Jan 07 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31303019"> <div itemprop="name">Mutations in <i>RHOT1</i> Disrupt Endoplasmic Reticulum-Mitochondria Contact Sites Interfering with Calcium Homeostasis and Mitochondrial Dynamics in Parkinson's Disease.</div> </a> <div itemprop="about">Antioxidants & redox signaling</div> <div itemprop="author">Grossmann D,Berenguer-Escuder C,Bellet ME,Scheibner D,Bohler J,Massart F,Rapaport D,Skupin A,Fouquier d'Hérouël A,Sharma M,Ghelfi J,Raković A,Lichtner P,Antony P,Glaab E,May P,Dimmer KS,Fitzgerald JC,Grünewald A,Krüger R</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Sun Dec 01 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30735723"> <div itemprop="name">FAM81A protein, a novel component of the postsynaptic density in adult brain.</div> </a> <div itemprop="about">Neuroscience letters</div> <div itemprop="author">Dosemeci A,Loo HK,Toy D,Winters CA,Reese TS,Tao-Cheng JH</div> <div itemprop="description">MA1-744 was used in Western Blotting to study the tissue and subcellular distribution of FAM81A protein and its expression levels during development.</div> <div itemprodp="datePublished">Tue Apr 23 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31488841"> <div itemprop="name">Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Hartman S,Liu Z,van Veen H,Vicente J,Reinen E,Martopawiro S,Zhang H,van Dongen N,Bosman F,Bassel GW,Visser EJW,Bailey-Serres J,Theodoulou FL,Hebelstrup KH,Gibbs DJ,Holdsworth MJ,Sasidharan R,Voesenek LACJ</div> <div itemprop="description">MA1-744 was used in Western Blotting to reveal the biological link between three gaseous signals for the regulation of flooding survival and identifies key regulatory targets for early stress perception that could be pivotal for developing flood-tolerant crops.</div> <div itemprodp="datePublished">Thu Sep 05 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31181810"> <div itemprop="name">Epigenetic Remodeling through Downregulation of Polycomb Repressive Complex 2 Mediates Chemotherapy Resistance in Testicular Germ Cell Tumors.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Singh R,Fazal Z,Corbet AK,Bikorimana E,Rodriguez JC,Khan EM,Shahid K,Freemantle SJ,Spinella MJ</div> <div itemprop="description">MA1-744 was used in Western Blotting to implicate histone methylation with chemosensitivity of Testicular Germ Cell Tumors.</div> <div itemprodp="datePublished">Sat Jun 08 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31346208"> <div itemprop="name">HER2/HER3 regulates lactate secretion and expression of lactate receptor mRNA through the MAP3K4 associated protein GIT1.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Garcia-Flores AE,Sollome JJ,Thavathiru E,Bower JL,Vaillancourt RR</div> <div itemprop="description">MA1-744 was used in Western Blotting to suggest that targeting downstream proteins in the HER2/HER3-induced extracellular lactate signaling pathway may be a way to inhibit the Warburg Effect to disrupt tumor growth.</div> <div itemprodp="datePublished">Thu Jul 25 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30842529"> <div itemprop="name">PARP1 is a versatile factor in the regulation of mRNA stability and decay.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Matveeva EA,Mathbout LF,Fondufe-Mittendorf YN</div> <div itemprop="description">MA1-744 was used in Western Blotting to examine the direct impact of PARP1 and PARylation on mRNA stability and decay.</div> <div itemprodp="datePublished">Wed Mar 06 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30777121"> <div itemprop="name">Coupling of PARP1-mediated chromatin structural changes to transcriptional RNA polymerase II elongation and cotranscriptional splicing.</div> </a> <div itemprop="about">Epigenetics & chromatin</div> <div itemprop="author">Matveeva EA,Al-Tinawi QMH,Rouchka EC,Fondufe-Mittendorf YN</div> <div itemprop="description">MA1-744 was used in Western Blotting to investigate the effect of PARP1-mediated chromatin changes on RNA polymerase II movement, during transcription and alternative splicing.</div> <div itemprodp="datePublished">Mon Feb 18 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30949049"> <div itemprop="name">The Inhibitory T Cell Receptors PD1 and 2B4 Are Differentially Regulated on CD4 and CD8 T Cells in a Mouse Model of Non-alcoholic Steatohepatitis.</div> </a> <div itemprop="about">Frontiers in pharmacology</div> <div itemprop="author">Hansel C,Erschfeld S,Baues M,Lammers T,Weiskirchen R,Trautwein C,Kroy DC,Drescher HK</div> <div itemprop="description">MA1-744 was used in Western Blotting to investigate the expression profile of inhibitory T cell receptors on CD4 and CD8 T cells in a mouse model of non-alcoholic steatohepatitis.</div> <div itemprodp="datePublished">Wed Sep 30 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28775143"> <div itemprop="name">OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development.</div> </a> <div itemprop="about">Plant physiology</div> <div itemprop="author">Burman N,Bhatnagar A,Khurana JP</div> <div itemprop="description">MA1-744 was used in Western Blotting to provide credible evidence that OsbZIP48 performs more diverse functions in a monocot system like rice in comparison with its Arabidopsis ortholog, HY5.</div> <div itemprodp="datePublished">Thu Feb 01 00:00:00 EST 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29343568"> <div itemprop="name">An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor.</div> </a> <div itemprop="about">Journal of virology</div> <div itemprop="author">Dudek AM,Pillay S,Puschnik AS,Nagamine CM,Cheng F,Qiu J,Carette JE,Vandenberghe LH</div> <div itemprop="description">MA1-744 was used in Western Blotting to identify a distinct adeno-associated virus (AAV) capsid lineage that can bind and transduce cells in the absence of AAV receptor, independent of the multiplicity of infection.</div> <div itemprodp="datePublished">Sun Apr 01 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30045857"> <div itemprop="name">Natural Variation in the Multidrug Efflux Pump <i>SGE1</i> Underlies Ionic Liquid Tolerance in Yeast.</div> </a> <div itemprop="about">Genetics</div> <div itemprop="author">Higgins DA,Young MKM,Tremaine M,Sardi M,Fletcher JM,Agnew M,Liu L,Dickinson Q,Peris D,Wrobel RL,Hittinger CT,Gasch AP,Singer SW,Simmons BA,Landick R,Thelen MP,Sato TK</div> <div itemprop="description">MA1-744 was used in Western Blotting to identify SGE1 and a previously uncharacterised gene names ILT1 which encodes a predicted membrane protein.</div> <div itemprodp="datePublished">Sat Sep 01 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30012212"> <div itemprop="name">MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection.</div> </a> <div itemprop="about">Veterinary research</div> <div itemprop="author">Qi X,Wang T,Xue Q,Li Z,Yang B,Wang J</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Mon Jul 16 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30233728"> <div itemprop="name">Combination treatment of captopril and prazosin to treat patients with gestational hypertension.</div> </a> <div itemprop="about">Experimental and therapeutic medicine</div> <div itemprop="author">Hong B,Ding X,Iia H,Zhang J</div> <div itemprop="description">MA1-744 was used in an ELISA assay to investigate captopril and prazosin combination treatment in patients with gestational hypertension.</div> <div itemprodp="datePublished">Mon Oct 01 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29891794"> <div itemprop="name">Polyphenols from <i>Tamarix nilotica</i>: LC⁻ESI-MS<sup>n</sup> Profiling and In Vivo Antifibrotic Activity.</div> </a> <div itemprop="about">Molecules (Basel, Switzerland)</div> <div itemprop="author">Sekkien A,Swilam N,Ebada SS,Esmat A,El-Khatib AH,Linscheid MW,Singab AN</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunohistochemistry</div> <div itemprodp="datePublished">Mon Jun 11 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30417124"> <div itemprop="name">Conserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats.</div> </a> <div itemprop="about">Communications biology</div> <div itemprop="author">Ostrowski LA,Hall AC,Szafranski KJ,Oshidari R,Abraham KJ,Chan JNY,Krustev C,Zhang K,Wang A,Liu Y,Guo R,Mekhail K</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Sat Jan 11 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29487133"> <div itemprop="name"><i>In silico</i> and crystallographic studies identify key structural features of biliverdin IXβ reductase inhibitors having nanomolar potency.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Nesbitt NM,Zheng X,Li Z,Manso JA,Yen WY,Malone LE,Ripoll-Rozada J,Pereira PJB,Mantle TJ,Wang J,Bahou WF</div> <div itemprop="description">MA1-744 was used in Western Blotting to study the structural features of biliverdin IXβ reductase inhibitors and reveal the mechanism of nanomolar potency.</div> <div itemprodp="datePublished">Fri Apr 13 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28835433"> <div itemprop="name">Integration of TRPC6 and NADPH oxidase activation in lysophosphatidylcholine-induced TRPC5 externalization.</div> </a> <div itemprop="about">American journal of physiology. Cell physiology</div> <div itemprop="author">Chaudhuri P,Rosenbaum MA,Birnbaumer L,Graham LM</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Wed Nov 01 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28568773"> <div itemprop="name">Cost-effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2α.</div> </a> <div itemprop="about">Yeast (Chichester, England)</div> <div itemprop="author">Lee SJ,Ramesh R,de Boor V,Gebler JM,Silva RC,Sattlegger E</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Fri Sep 01 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27574117"> <div itemprop="name">Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids.</div> </a> <div itemprop="about">Nucleic acids research</div> <div itemprop="author">Abraham KJ,Chan JN,Salvi JS,Ho B,Hall A,Vidya E,Guo R,Killackey SA,Liu N,Lee JE,Brown GW,Mekhail K</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Fri Oct 14 00:00:00 EDT 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27574117"> <div itemprop="name">Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids.</div> </a> <div itemprop="about">Nucleic acids research</div> <div itemprop="author">Abraham KJ,Chan JN,Salvi JS,Ho B,Hall A,Vidya E,Guo R,Killackey SA,Liu N,Lee JE,Brown GW,Mekhail K</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Fri Oct 14 00:00:00 EDT 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27760804"> <div itemprop="name">The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria.</div> </a> <div itemprop="about">The Plant cell</div> <div itemprop="author">Sultan LD,Mileshina D,Grewe F,Rolle K,Abudraham S,Głodowicz P,Niazi AK,Keren I,Shevtsov S,Klipcan L,Barciszewski J,Mower JP,Dietrich A,Ostersetzer-Biran O</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue Nov 01 00:00:00 EDT 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27208232"> <div itemprop="name">The GSK3/Shaggy-Like Kinase ASKα Contributes to Pattern-Triggered Immunity.</div> </a> <div itemprop="about">Plant physiology</div> <div itemprop="author">Stampfl H,Fritz M,Dal Santo S,Jonak C</div> <div itemprop="description">MA1-744 was used in western blot to study the contribution to pattern-triggered immunity from the GSK3/Shaggy-like kinase ASKalpha</div> <div itemprodp="datePublished">Wed Jun 01 00:00:00 EDT 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27280719"> <div itemprop="name">Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Ambrosi C,Ren C,Spagnol G,Cavin G,Cone A,Grintsevich EE,Sosinsky GE,Sorgen PL</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunoprecipitation</div> <div itemprodp="datePublished">Thu Jul 27 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27171244"> <div itemprop="name">Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Yu P,Ji L,Lee KJ,Yu M,He C,Ambati S,McKinney EC,Jackson C,Baile CA,Schmitz RJ,Meagher RB</div> <div itemprop="description">MA1744 was used in western blot to test if adipose tissues have epigenetically distinct subpopulations of adipocytes</div> <div itemprodp="datePublished">Mon Jul 10 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27481099"> <div itemprop="name">A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro.</div> </a> <div itemprop="about">DNA repair</div> <div itemprop="author">Fritzen R,Delbos F,De Smet A,Palancade B,Canman CE,Aoufouchi S,Weill JC,Reynaud CA,Storck S</div> <div itemprop="description">MA1744 was used in western blot to make mutant mice to determine the impact of REV3L catalytic activity</div> <div itemprodp="datePublished">Sat Oct 01 00:00:00 EDT 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27068241"> <div itemprop="name">Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Miao Y,Han X,Zheng L,Xie Y,Mu Y,Yates JR,Drubin DG</div> <div itemprop="description">MA1-744 was used in western blot to regulating actin cable dynamics in budding yeast by fimbrin phosphorylation by metaphase Cdk1</div> <div itemprodp="datePublished">Tue Apr 12 00:00:00 EDT 2016</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26157113"> <div itemprop="name">A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light.</div> </a> <div itemprop="about">Plant physiology</div> <div itemprop="author">Jones MA,Hu W,Litthauer S,Lagarias JC,Harmer SL</div> <div itemprop="description">MA1-744 was used in western blot to distinguish the effects of photoreceptor signaling on clock function from those of photosynthesis</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/25615019"> <div itemprop="name">A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis.</div> </a> <div itemprop="about">Traffic (Copenhagen, Denmark)</div> <div itemprop="author">Feliciano D,Tolsma TO,Farrell KB,Aradi A,Di Pietro SM</div> <div itemprop="description">MA1-744 was used in western blot to characterize the Las17 G-actin-binding motif in vitro and in vivo.</div> <div itemprodp="datePublished">Wed Apr 01 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26747770"> <div itemprop="name">Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Umazume T,Thomas WM,Campbell S,Aluri H,Thotakura S,Zoukhri D,Makarenkova HP</div> <div itemprop="description">MA1-744 was used in western blot to identify factors that are altered in the lacrimal gland by comparing several mouse models of disease with healthy mice</div> <div itemprodp="datePublished">Tue Dec 01 00:00:00 EST 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/25815878"> <div itemprop="name">Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2.</div> </a> <div itemprop="about">Molecular medicine reports</div> <div itemprop="author">Lee SM,Chang JY,Wu JS,Sheu DC</div> <div itemprop="description">MA1-744 was used in western blot examine the effects of neokestose on cell proliferation, cell cycle, and apoptosis of colonic cells</div> <div itemprodp="datePublished">Wed Jul 01 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26205667"> <div itemprop="name">Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Chung DK,Chan JN,Strecker J,Zhang W,Ebrahimi-Ardebili S,Lu T,Abraham KJ,Durocher D,Mekhail K</div> <div itemprop="description">MA1-744 was used in western blot to report roles for kinesin and nuclear pore complexes in DNA repair by break-induced replication</div> <div itemprodp="datePublished">Fri Jul 24 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/24486762"> <div itemprop="name">Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness.</div> </a> <div itemprop="about">Plant & cell physiology</div> <div itemprop="author">Maruyama D,Sugiyama T,Endo T,Nishikawa S</div> <div itemprop="description">MA1-744 was used in western blot to study the role of BiP genes in gametogenesis and pollen quality in Arabidopsis thaliana</div> <div itemprodp="datePublished">Tue Apr 01 00:00:00 EDT 2014</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/24036211"> <div itemprop="name">HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4).</div> </a> <div itemprop="about">Cellular signalling</div> <div itemprop="author">Sollome JJ,Thavathiru E,Camenisch TD,Vaillancourt RR</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Wed Jan 01 00:00:00 EST 2014</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23108789"> <div itemprop="name">Sex-dependent differences in rat brown adipose tissue mitochondrial biogenesis and insulin signaling parameters in response to an obesogenic diet.</div> </a> <div itemprop="about">Molecular and cellular biochemistry</div> <div itemprop="author">Nadal-Casellas A,Bauzá-Thorbrügge M,Proenza AM,Gianotti M,Lladó I</div> <div itemprop="description">MA1-744 was used in western blot to study gender differences in insulin signaling and brown adipose tissue mitochondrial biogenesis in response to diet-induced obesity in rats</div> <div itemprodp="datePublished">Tue Jan 01 00:00:00 EST 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23530031"> <div itemprop="name">Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Kasaai B,Gaumond MH,Moffatt P</div> <div itemprop="description">MA1-744 was used in western blot to study the role of Sp1, Sp3, OSX, and GLI2 and of CpG methylation status in the transcription of the bone-spefic membrane protein BRIL</div> <div itemprodp="datePublished">Fri May 10 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23530031"> <div itemprop="name">Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Kasaai B,Gaumond MH,Moffatt P</div> <div itemprop="description">MA1-744 was used in western blot to study the role of Sp1, Sp3, OSX, and GLI2 and of CpG methylation status in the transcription of the bone-spefic membrane protein BRIL</div> <div itemprodp="datePublished">Fri May 10 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23530031"> <div itemprop="name">Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Kasaai B,Gaumond MH,Moffatt P</div> <div itemprop="description">MA1-744 was used in western blot to study the role of Sp1, Sp3, OSX, and GLI2 and of CpG methylation status in the transcription of the bone-spefic membrane protein BRIL</div> <div itemprodp="datePublished">Fri May 10 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23369209"> <div itemprop="name">Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage.</div> </a> <div itemprop="about">Journal of translational medicine</div> <div itemprop="author">Tai CJ,Su TC,Jiang MC,Chen HC,Shen SC,Lee WR,Liao CF,Chen YC,Lin SH,Li LT,Shen KH,Yeh CM,Yeh KT,Lee CH,Shih HY,Chang CC</div> <div itemprop="description">MA1-744 was used in western blot to study the prognostic significance of immunohistochemical expression of cytoplasmic CSE1L in colorectal cancer</div> <div itemprodp="datePublished">Thu Jan 31 00:00:00 EST 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23306199"> <div itemprop="name">Cellular interactions of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Gargi A,Tamilselvam B,Powers B,Prouty MG,Lincecum T,Eshraghi A,Maldonado-Arocho FJ,Wilson BA,Bradley KA,Blanke SR</div> <div itemprop="description">MA1-744 was used in western blot to compare the molecular mechanisms of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi</div> <div itemprodp="datePublished">Fri Mar 15 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23521888"> <div itemprop="name">Protein tyrosine phosphatase-1B regulates the tyrosine phosphorylation of the adapter Grb2-associated binder 1 (Gab1) in the retina.</div> </a> <div itemprop="about">Cell communication and signaling : CCS</div> <div itemprop="author">Rajala A,Dilly AK,Rajala RV</div> <div itemprop="description">MA1-744 was used in western blot to characterize tyrosine phosphorylation of Gab1 in the retina</div> <div itemprodp="datePublished">Fri Mar 22 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/23400775"> <div itemprop="name">Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">McCoy F,Darbandi R,Chen SI,Eckard L,Dodd K,Jones K,Baucum AJ,Gibbons JA,Lin SH,Colbran RJ,Nutt LK</div> <div itemprop="description">MA1-744 was used in western blot to study metabolic regulation of CaMKII protein and caspases in Xenopus</div> <div itemprodp="datePublished">Fri Mar 29 00:00:00 EDT 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22863008"> <div itemprop="name">HSF1 drives a transcriptional program distinct from heat shock to support highly malignant human cancers.</div> </a> <div itemprop="about">Cell</div> <div itemprop="author">Mendillo ML,Santagata S,Koeva M,Bell GW,Hu R,Tamimi RM,Fraenkel E,Ince TA,Whitesell L,Lindquist S</div> <div itemprop="description">MA1-744 was used in western blot to study the role of HSF1 in driving a transcriptional program distinct from heat shock in highly malignant human cancers</div> <div itemprodp="datePublished">Fri Aug 03 00:00:00 EDT 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22021706"> <div itemprop="name">Toll-like receptor 4 promotes tubular inflammation in diabetic nephropathy.</div> </a> <div itemprop="about">Journal of the American Society of Nephrology : JASN</div> <div itemprop="author">Lin M,Yiu WH,Wu HJ,Chan LY,Leung JC,Au WS,Chan KW,Lai KN,Tang SC</div> <div itemprop="description">MA1-744 was used in western blot to study the role of toll-like receptors in diabetic nephropathy</div> <div itemprodp="datePublished">Sun Jan 01 00:00:00 EST 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22589468"> <div itemprop="name">Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists.</div> </a> <div itemprop="about">The Plant cell</div> <div itemprop="author">Kandasamy MK,McKinney EC,Roy E,Meagher RB</div> <div itemprop="description">MA1-744 was used in western blot to study the conserved functional competence of cytoplasmic actins</div> <div itemprodp="datePublished">Tue May 01 00:00:00 EDT 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22327741"> <div itemprop="name">Chitinase-like1/pom-pom1 and its homolog CTL2 are glucan-interacting proteins important for cellulose biosynthesis in Arabidopsis.</div> </a> <div itemprop="about">The Plant cell</div> <div itemprop="author">Sánchez-Rodríguez C,Bauer S,Hématy K,Saxe F,Ibáñez AB,Vodermaier V,Konlechner C,Sampathkumar A,Rüggeberg M,Aichinger E,Neumetzler L,Burgert I,Somerville C,Hauser MT,Persson S</div> <div itemprop="description">MA1-744 was used in western blot to investigate the functions of chitinase-like1/pom-pom1 and CTL2 in cellulose biosynthesis</div> <div itemprodp="datePublished">Wed Feb 01 00:00:00 EST 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22778136"> <div itemprop="name">GATA-1 utilizes Ikaros and polycomb repressive complex 2 to suppress Hes1 and to promote erythropoiesis.</div> </a> <div itemprop="about">Molecular and cellular biology</div> <div itemprop="author">Ross J,Mavoungou L,Bresnick EH,Milot E</div> <div itemprop="description">MA1-744 was used in western blot to study the molecular mechanism underlying the supression of HES-1 by GATA-1 and the significance for erythropoiesis</div> <div itemprodp="datePublished">Sat Sep 01 00:00:00 EDT 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22138962"> <div itemprop="name">Relative contributions of afferent vagal fibers to resistance to diet-induced obesity.</div> </a> <div itemprop="about">Digestive diseases and sciences</div> <div itemprop="author">Stearns AT,Balakrishnan A,Radmanesh A,Ashley SW,Rhoads DB,Tavakkolizadeh A</div> <div itemprop="description">MA1-744 was used in western blot to study the role of different afferent vagal fibres in mediating resistance to diet-induced obesity in a rat model</div> <div itemprodp="datePublished">Tue May 01 00:00:00 EDT 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22253827"> <div itemprop="name">Unique properties of eukaryote-type actin and profilin horizontally transferred to cyanobacteria.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Guljamow A,Delissen F,Baumann O,Thünemann AF,Dittmann E</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue May 29 00:00:00 EDT 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22253827"> <div itemprop="name">Unique properties of eukaryote-type actin and profilin horizontally transferred to cyanobacteria.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Guljamow A,Delissen F,Baumann O,Thünemann AF,Dittmann E</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Tue May 29 00:00:00 EDT 2012</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/22870341"> <div itemprop="name">The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Haaß W,Stehle M,Nittka S,Giehl M,Schrotz-King P,Fabarius A,Hofmann WK,Seifarth W</div> <div itemprop="description">MA1-744 was used in western blot to study the effect of imatinib on separase proteolytic activity in BCR-ABL-positive and -negative cells</div> <div itemprodp="datePublished">Tue Feb 19 00:00:00 EST 2013</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21285944"> <div itemprop="name">Small RNA-mediated regulation of iPS cell generation.</div> </a> <div itemprop="about">The EMBO journal</div> <div itemprop="author">Li Z,Yang CS,Nakashima K,Rana TM</div> <div itemprop="description">MA1-744 was used in western blot to study the role of cellular microRNAs in regulating the generation of inducible pluripotent stem cells</div> <div itemprodp="datePublished">Wed Mar 02 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21730149"> <div itemprop="name">Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies.</div> </a> <div itemprop="about">Proceedings of the National Academy of Sciences of the United States of America</div> <div itemprop="author">Selesniemi K,Lee HJ,Muhlhauser A,Tilly JL</div> <div itemprop="description">MA1-744 was used in western blot to study the effects of dietary restriction and PGC-1alpha deletion on age-related decreases in oocyte quality in mice</div> <div itemprodp="datePublished">Tue Jul 26 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21183956"> <div itemprop="name">SUMO-specific protease 2 in Mdm2-mediated regulation of p53.</div> </a> <div itemprop="about">Cell death and differentiation</div> <div itemprop="author">Jiang M,Chiu SY,Hsu W</div> <div itemprop="description">MA1-744 was used in western blot to study Mdm2-mediated regulation of p53</div> <div itemprodp="datePublished">Wed Jun 01 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21317879"> <div itemprop="name">Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis.</div> </a> <div itemprop="about">Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc</div> <div itemprop="author">Hizaki K,Yamamoto H,Taniguchi H,Adachi Y,Nakazawa M,Tanuma T,Kato N,Sukawa Y,Sanchez JV,Suzuki H,Sasaki S,Imai K,Shinomura Y</div> <div itemprop="description">MA1-744 was used in western blot to characterize alterations in calcium-sensing receptor in colorectal carcinogenesis</div> <div itemprodp="datePublished">Wed Jun 01 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21680521"> <div itemprop="name">Expression and functional characterization of the RIG-I-like receptors MDA5 and LGP2 in Rainbow trout (Oncorhynchus mykiss).</div> </a> <div itemprop="about">Journal of virology</div> <div itemprop="author">Chang M,Collet B,Nie P,Lester K,Campbell S,Secombes CJ,Zou J</div> <div itemprop="description">MA1-744 was used in western blot to characterize RIG-1-like receptors in Rainbow trout</div> <div itemprodp="datePublished">Mon Aug 01 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21109018"> <div itemprop="name">The roles of Kruppel-like factor 6 and peroxisome proliferator-activated receptor-γ in the regulation of macrophage inflammatory protein-3α at early onset of diabetes.</div> </a> <div itemprop="about">The international journal of biochemistry & cell biology</div> <div itemprop="author">Qi W,Holian J,Tan CY,Kelly DJ,Chen XM,Pollock CA</div> <div itemprop="description">MA1-744 was used in western blot to study the transcriptional regulation of MIP3-alpha by KLF6 and PPAR-gamma and the significance for diabetic retinopathy</div> <div itemprodp="datePublished">Tue Mar 01 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21482645"> <div itemprop="name">Effect of brimonidine on retinal and choroidal neovascularization in a mouse model of retinopathy of prematurity and laser-treated rats.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Kusari J,Padillo E,Zhou SX,Bai Y,Wang J,Song Z,Zhu M,Le YZ,Gil DW</div> <div itemprop="description">MA1-744 was used in western blot to investigate the effect of chronic brimonidine treatment on retinal and choroidal neovascularization</div> <div itemprodp="datePublished">Wed Jul 20 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21839079"> <div itemprop="name">Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells.</div> </a> <div itemprop="about">Experimental cell research</div> <div itemprop="author">Coso S,Zeng Y,Sooraj D,Williams ED</div> <div itemprop="description">MA1-744 was used in western blot to study the conservation of VEGF-R family signalling in lymphocytic endothelial cells from mice</div> <div itemprodp="datePublished">Sat Oct 15 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21256200"> <div itemprop="name">High content imaging-based assay to classify estrogen receptor-α ligands based on defined mechanistic outcomes.</div> </a> <div itemprop="about">Gene</div> <div itemprop="author">Ashcroft FJ,Newberg JY,Jones ED,Mikic I,Mancini MA</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Western Blot</div> <div itemprodp="datePublished">Sun May 15 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21315773"> <div itemprop="name">Human T-cell leukemia virus type 1 tax transactivates the matrix metalloproteinase 7 gene via JunD/AP-1 signaling.</div> </a> <div itemprop="about">Biochimica et biophysica acta</div> <div itemprop="author">Nakachi S,Nakazato T,Ishikawa C,Kimura R,Mann DA,Senba M,Masuzaki H,Mori N</div> <div itemprop="description">MA1-744 was used in western blot to study the molecular mechanism by which HTLV-1 transactivates MMP-7 gene expression</div> <div itemprodp="datePublished">Sun May 01 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21554739"> <div itemprop="name">Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models.</div> </a> <div itemprop="about">BMC research notes</div> <div itemprop="author">Matsuo T,Nishizuka SS,Ishida K,Iwaya T,Ikeda M,Wakabayashi G</div> <div itemprop="description">MA1-744 was used in western blot to study the mechanism of the anti-tumor activity of the EGFR inhibitor cetuximab</div> <div itemprodp="datePublished">Tue May 10 00:00:00 EDT 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/21829510"> <div itemprop="name">3',4'-Dihydroxyflavonol antioxidant attenuates diastolic dysfunction and cardiac remodeling in streptozotocin-induced diabetic m(Ren2)27 rats.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Khong FL,Zhang Y,Edgley AJ,Qi W,Connelly KA,Woodman OL,Krum H,Kelly DJ</div> <div itemprop="description">MA1-744 was used in western blot to study whether a synthetic flavonol antioxidant can protect against diabetic cardiomyopathy in a rat model of diabetes</div> <div itemprodp="datePublished">Tue Dec 06 00:00:00 EST 2011</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20395849"> <div itemprop="name">Rapid upregulation of sodium-glucose transporter SGLT1 in response to intestinal sweet taste stimulation.</div> </a> <div itemprop="about">Annals of surgery</div> <div itemprop="author">Stearns AT,Balakrishnan A,Rhoads DB,Tavakkolizadeh A</div> <div itemprop="description">MA1-744 was used in western blot to study the short-term regulation of intestinal SGLT-1 by glucose and sweet taste molecules</div> <div itemprodp="datePublished">Sat May 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20071566"> <div itemprop="name">Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells.</div> </a> <div itemprop="about">Journal of virology</div> <div itemprop="author">Luo MH,Hannemann H,Kulkarni AS,Schwartz PH,O'Dowd JM,Fortunato EA</div> <div itemprop="description">MA1-744 was used in western blot to study the effects of human CMV infection on the differentiation of human neural progenitor cells</div> <div itemprodp="datePublished">Thu Apr 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20504914"> <div itemprop="name">Novel small-molecule inhibitor of apurinic/apyrimidinic endonuclease 1 blocks proliferation and reduces viability of glioblastoma cells.</div> </a> <div itemprop="about">The Journal of pharmacology and experimental therapeutics</div> <div itemprop="author">Bapat A,Glass LS,Luo M,Fishel ML,Long EC,Georgiadis MM,Kelley MR</div> <div itemprop="description">MA1-744 was used in western blot to identify novel small molecule inhbitors of apurinic/apyrimidinic endonuclease-1 and study their therapeutic potential in glioblastoma</div> <div itemprodp="datePublished">Wed Sep 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20089883"> <div itemprop="name">Interdependence of platelet-derived growth factor and estrogen-signaling pathways in inducing neonatal rat testicular gonocytes proliferation.</div> </a> <div itemprop="about">Biology of reproduction</div> <div itemprop="author">Thuillier R,Mazer M,Manku G,Boisvert A,Wang Y,Culty M</div> <div itemprop="description">MA1-744 was used in western blot to study the interdependence of PDGF and estrogen signaling and their role in gonocyte development</div> <div itemprodp="datePublished">Sat May 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20070312"> <div itemprop="name">Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection.</div> </a> <div itemprop="about">Cellular microbiology</div> <div itemprop="author">Lapointe TK,O'Connor PM,Jones NL,Menard D,Buret AG</div> <div itemprop="description">MA1-744 was used in western blot to study the role of IL-1RI and Rho kinase in the mechanism by which Helicobacter pylori infection disrupts gastric epithelial tight junctions</div> <div itemprodp="datePublished">Sat May 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20862689"> <div itemprop="name">Differential sublocalization of actin variants within the nucleus.</div> </a> <div itemprop="about">Cytoskeleton (Hoboken, N.J.)</div> <div itemprop="author">Kandasamy MK,McKinney EC,Meagher RB</div> <div itemprop="description">Published figure using Actin monoclonal antibody (Product # MA1-744) in Immunofluorescence</div> <div itemprodp="datePublished">Mon Nov 01 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20979663"> <div itemprop="name">Influence of wild-type MLL on glucocorticoid sensitivity and response to DNA-damage in pediatric acute lymphoblastic leukemia.</div> </a> <div itemprop="about">Molecular cancer</div> <div itemprop="author">Beesley AH,Rampellini JL,Palmer ML,Heng JY,Samuels AL,Firth MJ,Ford J,Kees UR</div> <div itemprop="description">MA1-744 was used in western blot to study the effect of wild-type MLL on the response to DNA damage and the sensitivity to glucocorticoids in pediatric acute lymphoblastic leukemia</div> <div itemprodp="datePublished">Thu Oct 28 00:00:00 EDT 2010</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19276348"> <div itemprop="name">Impaired turnover of prolactin receptor contributes to transformation of human breast cells.</div> </a> <div itemprop="about">Cancer research</div> <div itemprop="author">Plotnikov A,Varghese B,Tran TH,Liu C,Rui H,Fuchs SY</div> <div itemprop="description">MA1-744 was used in western blot to study the increased proliferation and invasiveness of human mammary epithelial cells expressing degradation-resistant prolactin receptor</div> <div itemprodp="datePublished">Wed Apr 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19789344"> <div itemprop="name">Overexpression of kinesins mediates docetaxel resistance in breast cancer cells.</div> </a> <div itemprop="about">Cancer research</div> <div itemprop="author">De S,Cipriano R,Jackson MW,Stark GR</div> <div itemprop="description">MA1-744 was used in western blot to study the role of kinesin overexpression in the resistance of breast cancer cells to docetaxol</div> <div itemprodp="datePublished">Thu Oct 15 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19526623"> <div itemprop="name">Influence of nephron mass and a phosphorylated 38 mitogen-activated protein kinase inhibitor on the development of early and long-term injury after renal warm ischaemia.</div> </a> <div itemprop="about">The British journal of surgery</div> <div itemprop="author">Jayle C,Faure JP,Thuillier R,Goujon JM,Richer JP,Hauet T</div> <div itemprop="description">MA1-744 was used in western blot to study the effect of inhibition of p38 MAPK and TNF-alpha production on damage caused by renal warm ischemia</div> <div itemprodp="datePublished">Wed Jul 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19710232"> <div itemprop="name">An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis.</div> </a> <div itemprop="about">Plant physiology</div> <div itemprop="author">Bernard DG,Cheng Y,Zhao Y,Balk J</div> <div itemprop="description">MA1-744 was used in western blot to study the functions of the mitochondrial ATP-binding cassette transporters.</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19624487"> <div itemprop="name">Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking.</div> </a> <div itemprop="about">Traffic (Copenhagen, Denmark)</div> <div itemprop="author">Bethani I,Werner A,Kadian C,Geumann U,Jahn R,Rizzoli SO</div> <div itemprop="description">MA1-744 was used in western blot to study the ability of cells to cope with SNARE depletion</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19575708"> <div itemprop="name">Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors.</div> </a> <div itemprop="about">Journal of neurochemistry</div> <div itemprop="author">Rajala RV,Rajala A,Brush RS,Rotstein NP,Politi LE</div> <div itemprop="description">MA1-744 was used in western blot to study insulin receptor signaling in developing photoreceptors</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19264886"> <div itemprop="name">Bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Moyer AL,Ramadan RT,Novosad BD,Astley R,Callegan MC</div> <div itemprop="description">MA1-744 was used in western blot to investigate blood-retina barrier permeability in experimental endophthalmitis</div> <div itemprodp="datePublished">Sat Aug 01 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19268775"> <div itemprop="name">FR167653 improves renal recovery and decreases inflammation and fibrosis after renal ischemia reperfusion injury.</div> </a> <div itemprop="about">Journal of vascular surgery</div> <div itemprop="author">Cau J,Favreau F,Zhang K,Febrer G,de la Motte GR,Ricco JB,Goujon JM,Hauet T</div> <div itemprop="description">MA1-744 was used in western blot to study the beneficial effects of inhibiting TNF-alpha and IL-1beta production in promoting recovery from renal reperfusion injury</div> <div itemprodp="datePublished">Sun Mar 01 00:00:00 EST 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19111751"> <div itemprop="name">Intracellularly expressed granulysin induced apoptosis in hepatoma cells and role of mitochondrial apoptotic pathway.</div> </a> <div itemprop="about">Cellular immunology</div> <div itemprop="author">Yi Z,Fu Y,Jin G,Li M,Zhang X,Song W</div> <div itemprop="description">MA1-744 was used in western blot to study the role of the mitochondrial pathway in the induction of apoptosis in hepatoma cells by intracellular granulysin</div> <div itemprodp="datePublished">Fri Mar 06 00:00:00 EST 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19251051"> <div itemprop="name">Retinal insulin receptor signaling in hyperosmotic stress.</div> </a> <div itemprop="about">Vitamins and hormones</div> <div itemprop="author">Rajala RV,Ivanovic I,Dilly AK</div> <div itemprop="description">MA1-744 was used in western blot to examine retinal insulin receptor signaling in hyperosmotic stress</div> <div itemprodp="datePublished">Tue Jun 23 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/19643733"> <div itemprop="name">SLIMMER (FHL1B/KyoT3) interacts with the proapoptotic protein Siva-1 (CD27BP) and delays skeletal myoblast apoptosis.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Cottle DL,McGrath MJ,Wilding BR,Cowling BS,Kane JM,D'Arcy CE,Holdsworth M,Hatzinisiriou I,Prescott M,Brown S,Mitchell CA</div> <div itemprop="description">MA1-744 was used in western blot to study the mechanism by which SLIMMER delays apoptosis of skeletal muscle myoblasts</div> <div itemprodp="datePublished">Fri Sep 25 00:00:00 EDT 2009</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18483470"> <div itemprop="name">Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines.</div> </a> <div itemprop="about">Autophagy</div> <div itemprop="author">Cullinane M,Gong L,Li X,Lazar-Adler N,Tra T,Wolvetang E,Prescott M,Boyce JD,Devenish RJ,Adler B</div> <div itemprop="description">MA1-744 was used in western blot to investigate the role of autophagy in the intracellular survival of Burkholderia pseudomallei</div> <div itemprodp="datePublished">Fri Aug 01 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18566464"> <div itemprop="name">Insulin growth factor 1 receptor/PI3K/AKT survival pathway in outer segment membranes of rod photoreceptors.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Dilly AK,Rajala RV</div> <div itemprop="description">MA1-744 was used in western blot to examine IGFR-1 signaling in rod outer segment membranes</div> <div itemprodp="datePublished">Sat Nov 01 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17647262"> <div itemprop="name">Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia.</div> </a> <div itemprop="about">Journal of cellular biochemistry</div> <div itemprop="author">Moffatt P,Smith CE,St-Arnaud R,Nanci A</div> <div itemprop="description">MA1-744 was used in western blot to characterize Apin</div> <div itemprodp="datePublished">Fri Feb 15 00:00:00 EST 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18504087"> <div itemprop="name">Tight junctional disruption and apoptosis in an in vitro model of Citrobacter rodentium infection.</div> </a> <div itemprop="about">Microbial pathogenesis</div> <div itemprop="author">Flynn AN,Buret AG</div> <div itemprop="description">MA1-744 was used in western blot to study the effects of Citrobacter rodentium on mouse intestinal epithelial cell tight junction protein expression and apoptosis</div> <div itemprodp="datePublished">Fri Aug 01 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/20151040"> <div itemprop="name">Phospho-Site-Specific Antibody Microarray to Study the State of Protein Phosphorylation in the Retina.</div> </a> <div itemprop="about">Journal of proteomics & bioinformatics</div> <div itemprop="author">Rajala RV</div> <div itemprop="description">MA1-744 was used in western blot to study protein phosphorylation in the retina</div> <div itemprodp="datePublished">Wed Aug 13 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18957412"> <div itemprop="name">The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Bych K,Netz DJ,Vigani G,Bill E,Lill R,Pierik AJ,Balk J</div> <div itemprop="description">MA1-744 was used in western blot to characterize a new plant P loop NTPase from Arabidopsis.</div> <div itemprodp="datePublished">Fri Dec 19 00:00:00 EST 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/18480052"> <div itemprop="name">Loss of neuroprotective survival signal in mice lacking insulin receptor gene in rod photoreceptor cells.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Rajala A,Tanito M,Le YZ,Kahn CR,Rajala RV</div> <div itemprop="description">MA1-744 was used in western blot to study the role of insulin receptor signaling in rod photoreceptor cells</div> <div itemprodp="datePublished">Fri Jul 11 00:00:00 EDT 2008</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17394579"> <div itemprop="name">Delay of photoreceptor degeneration in tubby mouse by sulforaphane.</div> </a> <div itemprop="about">Journal of neurochemistry</div> <div itemprop="author">Kong L,Tanito M,Huang Z,Li F,Zhou X,Zaharia A,Yodoi J,McGinnis JF,Cao W</div> <div itemprop="description">MA1-744 was used in western blot to examine the effects of sulforaphane on photoreceptor degeneration in the tubby mouse</div> <div itemprodp="datePublished">Tue May 01 00:00:00 EDT 2007</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17196446"> <div itemprop="name">Role of Claspin in regulation of nucleotide excision repair factor DDB2.</div> </a> <div itemprop="about">DNA repair</div> <div itemprop="author">Praetorius-Ibba M,Wang QE,Wani G,El-Mahdy MA,Zhu Q,Qin S,Wani AA</div> <div itemprop="description">MA1-744 was used in western blot to study the modulation of the nucleotide excision-repair factor DDB2 by the replication checkpoint protein Claspin</div> <div itemprodp="datePublished">Tue May 01 00:00:00 EDT 2007</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17851176"> <div itemprop="name">Cholesterol secoaldehyde, an ozonation product of cholesterol, induces amyloid aggregation and apoptosis in murine GT1-7 hypothalamic neurons.</div> </a> <div itemprop="about">Journal of Alzheimer's disease : JAD</div> <div itemprop="author">Sathishkumar K,Xi X,Martin R,Uppu RM</div> <div itemprop="description">MA1-744 was used in western blot to investigate possible links between cholesterol secoaldehyde and amyloid aggregation</div> <div itemprodp="datePublished">Fri Jun 01 00:00:00 EDT 2007</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17706942"> <div itemprop="name">Altered development of neuronal progenitor cells after stimulation with autistic blood sera.</div> </a> <div itemprop="about">Brain research</div> <div itemprop="author">Mazur-Kolecka B,Cohen IL,Jenkins EC,Kaczmarski W,Flory M,Frackowiak J</div> <div itemprop="description">MA1-744 was used in western blot to examine the effects of autistic blood serum on development of neuronal progenitor cells in vitro</div> <div itemprodp="datePublished">Fri Sep 07 00:00:00 EDT 2007</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17672890"> <div itemprop="name">Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis.</div> </a> <div itemprop="about">Comparative hepatology</div> <div itemprop="author">Spee B,Arends B,van den Ingh TS,Roskams T,Rothuizen J,Penning LC</div> <div itemprop="description">MA1-744 was used in western blot to study the similarities in HGF-mediated regeneration pathway activation in cirrhosis in humans and dogs</div> <div itemprodp="datePublished">Tue Jul 31 00:00:00 EDT 2007</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/16618411"> <div itemprop="name">Gastrin increases murine intestinal crypt regeneration following injury.</div> </a> <div itemprop="about">Gastroenterology</div> <div itemprop="author">Ottewell PD,Duckworth CA,Varro A,Dimaline R,Wang TC,Watson AJ,Dockray GJ,Pritchard DM</div> <div itemprop="description">MA1-744 was used in western blot to study the effects of gastrin on the recovery of murine intestinal crypts from injury</div> <div itemprodp="datePublished">Sat Apr 01 00:00:00 EST 2006</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17145872"> <div itemprop="name">Mesenchymal-to-epithelial transition facilitates bladder cancer metastasis: role of fibroblast growth factor receptor-2.</div> </a> <div itemprop="about">Cancer research</div> <div itemprop="author">Chaffer CL,Brennan JP,Slavin JL,Blick T,Thompson EW,Williams ED</div> <div itemprop="description">MA1-744 was used in immunocytochemistry to study the role of FGF-R2 in the mesenchymal-epithelial transition and bladder cancer metastasis</div> <div itemprodp="datePublished">Fri Dec 01 00:00:00 EST 2006</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/17122152"> <div itemprop="name">Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress.</div> </a> <div itemprop="about">Investigative ophthalmology & visual science</div> <div itemprop="author">Zheng L,Anderson RE,Agbaga MP,Rucker EB,Le YZ</div> <div itemprop="description">MA1-744 was used in immunohistochemistry to study the effect of Bcl-x disruption on in rod photoreceptor susceptibility to bright light stress.</div> <div itemprodp="datePublished">Fri Dec 01 00:00:00 EST 2006</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/16926123"> <div itemprop="name">Hydroxycamptothecin-induced apoptosis in hepatoma SMMC-7721 cells and the role of mitochondrial pathway.</div> </a> <div itemprop="about">Mitochondrion</div> <div itemprop="author">Fu YR,Yi ZJ,Yan YR,Qiu ZY</div> <div itemprop="description">MA1-744 was used in western blot to study the involvement of the mitochondrial pathway in the induction of hepatoma apoptosis by the topoisomerase I inhibitor hydroxycamptothecin</div> <div itemprodp="datePublished">Tue Aug 01 00:00:00 EDT 2006</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/16595676"> <div itemprop="name">Knocking down type 2 but not type 1 calsequestrin reduces calcium sequestration and release in C2C12 skeletal muscle myotubes.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Wang Y,Xu L,Duan H,Pasek DA,Eu JP,Meissner G</div> <div itemprop="description">MA1-744 was used in western blot to study the roles of type 1 and type 2 calsequestrins in stored Ca2+ release by C2C12 skeletal muscle myotubes</div> <div itemprodp="datePublished">Fri Jun 02 00:00:00 EDT 2006</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15930315"> <div itemprop="name">The heat shock protein 90 inhibitor, 17-allylamino-17-demethoxygeldanamycin, enhances osteoclast formation and potentiates bone metastasis of a human breast cancer cell line.</div> </a> <div itemprop="about">Cancer research</div> <div itemprop="author">Price JT,Quinn JM,Sims NA,Vieusseux J,Waldeck K,Docherty SE,Myers D,Nakamura A,Waltham MC,Gillespie MT,Thompson EW</div> <div itemprop="description">MA1-744 was used in western blot to study the ability of a HSP90 inhibitor to promote osteoclast formation and bone metastasis of human breast cancer cells</div> <div itemprodp="datePublished">Wed Jun 01 00:00:00 EDT 2005</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15729715"> <div itemprop="name">Transcriptional activation of survivin through the NF-kappaB pathway by human T-cell leukemia virus type I tax.</div> </a> <div itemprop="about">International journal of cancer</div> <div itemprop="author">Kawakami H,Tomita M,Matsuda T,Ohta T,Tanaka Y,Fujii M,Hatano M,Tokuhisa T,Mori N</div> <div itemprop="description">MA1-744 was used in western blot to study the role of HTLV-1 tax in the transcriptional activation of survivin and the involvement of the NFkappaB pathway</div> <div itemprodp="datePublished">Wed Jul 20 00:00:00 EDT 2005</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/16260419"> <div itemprop="name">Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNK.</div> </a> <div itemprop="about">The Journal of biological chemistry</div> <div itemprop="author">Zhao KW,Li D,Zhao Q,Huang Y,Silverman RH,Sims PJ,Chen GQ</div> <div itemprop="description">MA1-744 was used in western blot to study the mechanism underlying STAT1-mediated expression of phospholipd scramblase-1 in response to IFN-alpha</div> <div itemprodp="datePublished">Fri Dec 30 00:00:00 EST 2005</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15196287"> <div itemprop="name">Th1-dominant shift of T cell cytokine production, and subsequent reduction of serum immunoglobulin E response by administration in vivo of plasmid expressing Txk/Rlk, a member of Tec family tyrosine kinases, in a mouse model.</div> </a> <div itemprop="about">Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology</div> <div itemprop="author">Takeno M,Yoshikawa H,Kurokawa M,Takeba Y,Kashiwakura JI,Sakaguchi M,Yasueda H,Suzuki N</div> <div itemprop="description">MA1-744 was used in western blot to study the effect of Txk/Rlk tyrosine kinase expression on the murine Th1/Th2 cytokine balance and serum IgE levels</div> <div itemprodp="datePublished">Tue Jun 01 00:00:00 EDT 2004</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15120641"> <div itemprop="name">Fibronectin-induced COX-2 mediates MMP-2 expression and invasiveness of rhabdomyosarcoma.</div> </a> <div itemprop="about">Biochemical and biophysical research communications</div> <div itemprop="author">Ito H,Duxbury M,Benoit E,Farivar RS,Gardner-Thorpe J,Zinner MJ,Ashley SW,Whang EE</div> <div itemprop="description">MA1-744 was used in western blot to study the role of fibronectin-inducible COX-2 and PGE2 in rhabdomyosarcoma invasiveness and MMP-2 expression</div> <div itemprodp="datePublished">Fri May 28 00:00:00 EDT 2004</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/15012721"> <div itemprop="name">Fibrogenic stresses activate different mitogen-activated protein kinase pathways in renal epithelial, endothelial or fibroblast cell populations.</div> </a> <div itemprop="about">Nephrology (Carlton, Vic.)</div> <div itemprop="author">Pat BK,Cuttle L,Watters D,Yang T,Johnson DW,Gobe GC</div> <div itemprop="description">MA1-744 was used in western blot to study the different MAP kinase pathways activated in renal epithelial, endothelial and fibroblast cells in response to fibrogenic stress</div> <div itemprodp="datePublished">Fri Aug 01 00:00:00 EDT 2003</div> </div> </noscript> </div> <div class="border-bottom"></div> <div class="product-bioinformatics-details"> <div class="container"> <h2 class="pdp-sub-heading">Bioinformatics</h2> <div class="row"> <div class="large-span-6 medium-span-12 small-span-12"> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="proteinContent"> <strong>Protein Aliases:</strong> 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; Act5C-PE; ACTB; actin; actin 5 C; actin 5c; actin A1; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; Actin-5C; actin-like protein; actin5C; alpha 1 skeletal muscle actin; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; anon-fast-evolving-2D2; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cellular cytoskeletal beta-actin; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; CG4027-PE; Cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; Dm-actin5C; epididymis luminal protein 176; F-actin; G-actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RE02927p; RP5-1068B5.2; sma; smooth muscle alpha-actin; Smooth muscle gamma-actin </p> <p class="view-buttons" id="ptnViewBtn" ng-class="$ctrl.bioContentLines('proteinContent', 'ptnViewBtn')"> <a id="proteinViewMoreBtn" ng-click="$ctrl.bioContentViewMore('proteinViewMoreBtn', 'proteinViewLessBtn', 'proteinContent')"> View more </a> <a id="proteinViewLessBtn" ng-click="$ctrl.bioContentViewLess('proteinViewLessBtn', 'proteinViewMoreBtn', 'proteinContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="geneContent"> <strong>Gene Aliases:</strong> 0610041G09Rik; A; a-SMA; A4V404_DROME; AA959943; AAT6; Ac5C; ACT; act 5C; Act-4; Act-5C; ACT1_DROME; Act4; Act5; ACT5C; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actin; Actin/BAP47; Actin5C; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; anon-EST:fe2D2; ArpA; ASD5; ASMA; BAP47; beta-actin; beta-actin/Bap47; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CG4027; chrX:5748184..5748304; CMD1R; CMH11; cyt5C; DFNA20; DFNA26; Dmel\CG4027; Dmel_CG4027; E430023M04Rik; E51; F-actin; G-actin; GIG46; HEL-176; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0177; l(1)G0245; l(1)G0330; l(1)G0420; l(1)G0486; LVNC4; M32055; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; T11; VSCM; wu:fb63d03; XELAEV_18029136mg; XELAEV_18045052mg </p> <p class="view-buttons" id="geneViewBtn" ng-class="$ctrl.bioContentLines('geneContent', 'geneViewBtn')"> <a id="geneViewMoreBtn" ng-click="$ctrl.bioContentViewMore('geneViewMoreBtn', 'geneViewLessBtn', 'geneContent')"> View more </a> <a id="geneViewLessBtn" ng-click="$ctrl.bioContentViewLess('geneViewLessBtn', 'geneViewMoreBtn', 'geneContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="unitProContent"> <strong>UniProt ID:</strong> <a href="http://www.uniprot.org/uniprot/P68133" target="_blank">(Human) P68133</a>, <a href="http://www.uniprot.org/uniprot/P68138" target="_blank">(Bovine) P68138</a>, <a href="http://www.uniprot.org/uniprot/P62739" target="_blank">(Bovine) P62739</a>, <a href="http://www.uniprot.org/uniprot/P62736" target="_blank">(Human) P62736</a>, <a href="http://www.uniprot.org/uniprot/P60712" target="_blank">(Bovine) P60712</a>, <a href="http://www.uniprot.org/uniprot/P60709" target="_blank">(Human) P60709</a>, <a href="http://www.uniprot.org/uniprot/P60713" target="_blank">(Sheep) P60713</a>, <a href="http://www.uniprot.org/uniprot/P68032" target="_blank">(Human) P68032</a>, <a href="http://www.uniprot.org/uniprot/Q3ZC07" target="_blank">(Bovine) Q3ZC07</a>, <a href="http://www.uniprot.org/uniprot/P63261" target="_blank">(Human) P63261</a>, <a href="http://www.uniprot.org/uniprot/P63258" target="_blank">(Bovine) P63258</a>, <a href="http://www.uniprot.org/uniprot/Q5E9B5" target="_blank">(Bovine) Q5E9B5</a>, <a href="http://www.uniprot.org/uniprot/P63267" target="_blank">(Human) P63267</a>, <a href="http://www.uniprot.org/uniprot/P10987" target="_blank">(Fruit fly) P10987</a>, <a href="http://www.uniprot.org/uniprot/P68134" target="_blank">(Mouse) P68134</a>, <a href="http://www.uniprot.org/uniprot/P68136" target="_blank">(Rat) P68136</a>, <a href="http://www.uniprot.org/uniprot/P62738" target="_blank">(Rat) P62738</a>, <a href="http://www.uniprot.org/uniprot/P62737" target="_blank">(Mouse) P62737</a>, <a href="http://www.uniprot.org/uniprot/P60711" target="_blank">(Rat) P60711</a>, <a href="http://www.uniprot.org/uniprot/P60710" target="_blank">(Mouse) P60710</a>, <a href="http://www.uniprot.org/uniprot/P68035" target="_blank">(Rat) P68035</a>, <a href="http://www.uniprot.org/uniprot/P68033" target="_blank">(Mouse) P68033</a>, <a href="http://www.uniprot.org/uniprot/P63260" target="_blank">(Mouse) P63260</a>, <a href="http://www.uniprot.org/uniprot/P63259" target="_blank">(Rat) P63259</a>, <a href="http://www.uniprot.org/uniprot/P63268" target="_blank">(Mouse) P63268</a>, <a href="http://www.uniprot.org/uniprot/P63269" target="_blank">(Rat) P63269</a>, <a href="http://www.uniprot.org/uniprot/O93400" target="_blank">(Xenopus) O93400</a> </p> <p class="view-buttons" id="unitViewBtn" ng-class="$ctrl.bioContentLines('unitProContent', 'unitViewBtn')"> <a id="unitProViewMoreBtn" ng-click="$ctrl.bioContentViewMore('unitProViewMoreBtn', 'unitProViewLessBtn', 'unitProContent')"> View more </a> <a id="unitProViewLessBtn" ng-click="$ctrl.bioContentViewLess('unitProViewLessBtn', 'unitProViewMoreBtn', 'unitProContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="entrezContent"> <strong>Entrez Gene ID:</strong> <a href="http://www.ncbi.nlm.nih.gov/gene?term=58" target="_blank">(Human) 58</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=281592" target="_blank">(Bovine) 281592</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=101107662" target="_blank">(Sheep) 101107662</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=515610" target="_blank">(Bovine) 515610</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=59" target="_blank">(Human) 59</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=101121051" target="_blank">(Sheep) 101121051</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=280979" target="_blank">(Bovine) 280979</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=60" target="_blank">(Human) 60</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=443052" target="_blank">(Sheep) 443052</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=70" target="_blank">(Human) 70</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=533219" target="_blank">(Bovine) 533219</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=101111554" target="_blank">(Sheep) 101111554</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=71" target="_blank">(Human) 71</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=404122" target="_blank">(Bovine) 404122</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=443340" target="_blank">(Sheep) 443340</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=101114256" target="_blank">(Sheep) 101114256</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=281595" target="_blank">(Bovine) 281595</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=72" target="_blank">(Human) 72</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=31521" target="_blank">(Fruit fly) 31521</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=11459" target="_blank">(Mouse) 11459</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=29437" target="_blank">(Rat) 29437</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=81633" target="_blank">(Rat) 81633</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=11475" target="_blank">(Mouse) 11475</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=81822" target="_blank">(Rat) 81822</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=11461" target="_blank">(Mouse) 11461</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=29275" target="_blank">(Rat) 29275</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=11464" target="_blank">(Mouse) 11464</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=11465" target="_blank">(Mouse) 11465</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=287876" target="_blank">(Rat) 287876</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=11468" target="_blank">(Mouse) 11468</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=25365" target="_blank">(Rat) 25365</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=432314" target="_blank">(Xenopus) 432314</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=322509" target="_blank">(Zebrafish) 322509</a>, <a href="http://www.ncbi.nlm.nih.gov/gene?term=398459" target="_blank">(Xenopus) 398459</a> </p> <p class="view-buttons" id="etzViewBtn" ng-class="$ctrl.bioContentLines('entrezContent', 'etzViewBtn')"> <a id="entrezViewMoreBtn" ng-click="$ctrl.bioContentViewMore('entrezViewMoreBtn', 'entrezViewLessBtn', 'entrezContent')"> View more </a> <a id="entrezViewLessBtn" ng-click="$ctrl.bioContentViewLess('entrezViewLessBtn', 'entrezViewMoreBtn', 'entrezContent')"> View less </a> </p> </div> </div> <div class="large-span-6 medium-span-12 small-span-12"> <div class="accordion" id="accordion-bioinformatics"> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseOne"> Function(s) </a> </div> <div id="collapseOne" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/structural constituent of cytoskeleton"> structural constituent of cytoskeleton </a> <a class="icon" href="/antibody/primary/panther/protein binding"> protein binding </a> <a class="icon" href="/antibody/primary/panther/ATP binding"> ATP binding </a> <a class="icon" href="/antibody/primary/panther/myosin binding"> myosin binding </a> <a class="icon" href="/antibody/primary/panther/ADP binding"> ADP binding </a> <a class="icon" href="/antibody/primary/panther/protein kinase binding"> protein kinase binding </a> <a class="icon" href="/antibody/primary/panther/kinesin binding"> kinesin binding </a> <a class="icon" href="/antibody/primary/panther/Tat protein binding"> Tat protein binding </a> <a class="icon" href="/antibody/primary/panther/identical protein binding"> identical protein binding </a> <a class="icon" href="/antibody/primary/panther/nitric-oxide synthase binding"> nitric-oxide synthase binding </a> <a class="icon" href="/antibody/primary/panther/RNA polymerase II core promoter proximal region sequence-specific DNA binding"> RNA polymerase II core promoter proximal region sequence-specific DNA binding </a> <a class="icon" href="/antibody/primary/panther/RNA polymerase II distal enhancer sequence-specific DNA binding"> RNA polymerase II distal enhancer sequence-specific DNA binding </a> <a class="icon" href="/antibody/primary/panther/nucleosomal DNA binding"> nucleosomal DNA binding </a> <a class="icon" href="/antibody/primary/panther/ATPase activity"> ATPase activity </a> <a class="icon" href="/antibody/primary/panther/ubiquitin protein ligase binding"> ubiquitin protein ligase binding </a> <a class="icon" href="/antibody/primary/panther/profilin binding"> profilin binding </a> <a class="icon" href="/antibody/primary/panther/nucleotide binding"> nucleotide binding </a> <a class="icon" href="/antibody/primary/panther/molecular_function"> molecular_function </a> <a class="icon" href="/antibody/primary/panther/actin and actin related protein"> actin and actin related protein </a> <a class="icon" href="/antibody/primary/panther/actin or actin-binding cytoskeletal protein"> actin or actin-binding cytoskeletal protein </a> <a class="icon" href="/antibody/primary/panther/cytoskeletal protein"> cytoskeletal protein </a> </div> </div> </div> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseTwo"> Process(es) </a> </div> <div id="collapseTwo" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/muscle contraction"> muscle contraction </a> <a class="icon" href="/antibody/primary/panther/response to mechanical stimulus"> response to mechanical stimulus </a> <a class="icon" href="/antibody/primary/panther/response to extracellular stimulus"> response to extracellular stimulus </a> <a class="icon" href="/antibody/primary/panther/response to lithium ion"> response to lithium ion </a> <a class="icon" href="/antibody/primary/panther/positive regulation of gene expression"> positive regulation of gene expression </a> <a class="icon" href="/antibody/primary/panther/cell growth"> cell growth </a> <a class="icon" href="/antibody/primary/panther/muscle filament sliding"> muscle filament sliding </a> <a class="icon" href="/antibody/primary/panther/skeletal muscle thin filament assembly"> skeletal muscle thin filament assembly </a> <a class="icon" href="/antibody/primary/panther/skeletal muscle fiber adaptation"> skeletal muscle fiber adaptation </a> <a class="icon" href="/antibody/primary/panther/response to steroid hormone"> response to steroid hormone </a> <a class="icon" href="/antibody/primary/panther/skeletal muscle fiber development"> skeletal muscle fiber development </a> <a class="icon" href="/antibody/primary/panther/mesenchyme migration"> mesenchyme migration </a> <a class="icon" href="/antibody/primary/panther/regulation of blood pressure"> regulation of blood pressure </a> <a class="icon" href="/antibody/primary/panther/response to virus"> response to virus </a> <a class="icon" href="/antibody/primary/panther/vascular smooth muscle contraction"> vascular smooth muscle contraction </a> <a class="icon" href="/antibody/primary/panther/glomerular mesangial cell development"> glomerular mesangial cell development </a> <a class="icon" href="/antibody/primary/panther/morphogenesis of a polarized epithelium"> morphogenesis of a polarized epithelium </a> <a class="icon" href="/antibody/primary/panther/establishment or maintenance of cell polarity"> establishment or maintenance of cell polarity </a> <a class="icon" href="/antibody/primary/panther/regulation of transmembrane transporter activity"> regulation of transmembrane transporter activity </a> <a class="icon" href="/antibody/primary/panther/negative regulation of protein binding"> negative regulation of protein binding </a> <a class="icon" href="/antibody/primary/panther/adherens junction assembly"> adherens junction assembly </a> <a class="icon" href="/antibody/primary/panther/apical protein localization"> apical protein localization </a> <a class="icon" href="/antibody/primary/panther/synaptic vesicle endocytosis"> synaptic vesicle endocytosis </a> <a class="icon" href="/antibody/primary/panther/regulation of norepinephrine uptake"> regulation of norepinephrine uptake </a> <a class="icon" href="/antibody/primary/panther/protein localization to adherens junction"> protein localization to adherens junction </a> <a class="icon" href="/antibody/primary/panther/cellular response to cytochalasin B"> cellular response to cytochalasin B </a> <a class="icon" href="/antibody/primary/panther/postsynaptic actin cytoskeleton organization"> postsynaptic actin cytoskeleton organization </a> <a class="icon" href="/antibody/primary/panther/regulation of protein localization to plasma membrane"> regulation of protein localization to plasma membrane </a> <a class="icon" href="/antibody/primary/panther/retina homeostasis"> retina homeostasis </a> <a class="icon" href="/antibody/primary/panther/movement of cell or subcellular component"> movement of cell or subcellular component </a> <a class="icon" href="/antibody/primary/panther/cytoskeleton organization"> cytoskeleton organization </a> <a class="icon" href="/antibody/primary/panther/substantia nigra development"> substantia nigra development </a> <a class="icon" href="/antibody/primary/panther/cell junction assembly"> cell junction assembly </a> <a class="icon" href="/antibody/primary/panther/Fc-gamma receptor signaling pathway involved in phagocytosis"> Fc-gamma receptor signaling pathway involved in phagocytosis </a> <a class="icon" href="/antibody/primary/panther/ATP-dependent chromatin remodeling"> ATP-dependent chromatin remodeling </a> <a class="icon" href="/antibody/primary/panther/positive regulation of gene expression, epigenetic"> positive regulation of gene expression, epigenetic </a> <a class="icon" href="/antibody/primary/panther/vascular endothelial growth factor receptor signaling pathway"> vascular endothelial growth factor receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/ephrin receptor signaling pathway"> ephrin receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/platelet aggregation"> platelet aggregation </a> <a class="icon" href="/antibody/primary/panther/apoptotic process"> apoptotic process </a> <a class="icon" href="/antibody/primary/panther/actin filament-based movement"> actin filament-based movement </a> <a class="icon" href="/antibody/primary/panther/actomyosin structure organization"> actomyosin structure organization </a> <a class="icon" href="/antibody/primary/panther/actin-myosin filament sliding"> actin-myosin filament sliding </a> <a class="icon" href="/antibody/primary/panther/response to drug"> response to drug </a> <a class="icon" href="/antibody/primary/panther/negative regulation of apoptotic process"> negative regulation of apoptotic process </a> <a class="icon" href="/antibody/primary/panther/response to ethanol"> response to ethanol </a> <a class="icon" href="/antibody/primary/panther/cardiac myofibril assembly"> cardiac myofibril assembly </a> <a class="icon" href="/antibody/primary/panther/cardiac muscle tissue morphogenesis"> cardiac muscle tissue morphogenesis </a> <a class="icon" href="/antibody/primary/panther/heart contraction"> heart contraction </a> <a class="icon" href="/antibody/primary/panther/cardiac muscle contraction"> cardiac muscle contraction </a> <a class="icon" href="/antibody/primary/panther/sarcomere organization"> sarcomere organization </a> <a class="icon" href="/antibody/primary/panther/angiogenesis"> angiogenesis </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cell migration"> positive regulation of cell migration </a> <a class="icon" href="/antibody/primary/panther/regulation of stress fiber assembly"> regulation of stress fiber assembly </a> <a class="icon" href="/antibody/primary/panther/regulation of focal adhesion assembly"> regulation of focal adhesion assembly </a> <a class="icon" href="/antibody/primary/panther/positive regulation of wound healing"> positive regulation of wound healing </a> <a class="icon" href="/antibody/primary/panther/tight junction assembly"> tight junction assembly </a> <a class="icon" href="/antibody/primary/panther/protein localization to bicellular tight junction"> protein localization to bicellular tight junction </a> <a class="icon" href="/antibody/primary/panther/mitotic cytokinesis"> mitotic cytokinesis </a> <a class="icon" href="/antibody/primary/panther/chromatin remodeling"> chromatin remodeling </a> <a class="icon" href="/antibody/primary/panther/sperm individualization"> sperm individualization </a> <a class="icon" href="/antibody/primary/panther/maintenance of protein location in cell"> maintenance of protein location in cell </a> <a class="icon" href="/antibody/primary/panther/tube formation"> tube formation </a> <a class="icon" href="/antibody/primary/panther/axonogenesis"> axonogenesis </a> <a class="icon" href="/antibody/primary/panther/retina development in camera-type eye"> retina development in camera-type eye </a> <a class="icon" href="/antibody/primary/panther/cellular response to electrical stimulus"> cellular response to electrical stimulus </a> <a class="icon" href="/antibody/primary/panther/metabolic process"> metabolic process </a> <a class="icon" href="/antibody/primary/panther/actin-mediated cell contraction"> actin-mediated cell contraction </a> <a class="icon" href="/antibody/primary/panther/response to calcium ion"> response to calcium ion </a> <a class="icon" href="/antibody/primary/panther/biological_process"> biological_process </a> <a class="icon" href="/antibody/primary/panther/blood circulation"> blood circulation </a> </div> </div> </div> </div> </div> </div> </div> </div> <div class="recommended-products border-bottom hidden">  It has to be done as per old AB suggested Products section. </div> <script type="text/javascript" src="/order/genome-database/assets/core/js/magellanCore.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript"> $m.addPerformanceMark('abPDPScripts:loadBegin'); </script> <script type="text/javascript" src="/order/genome-database/assets/js/shared-module/dist/angular108-document-scripts.js?version=Local&time=20240917&build-time=2024-11-22T02:34:20+0000"></script> <script type="text/javascript" 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