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Boerl. and its processed commercial products" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/117849423/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products">Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products</a></div><div class="wp-workCard_item"><span>Materials Today: Proceedings</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short D...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="117849423"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="117849423"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 117849423; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=117849423]").text(description); $(".js-view-count[data-work-id=117849423]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 117849423; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='117849423']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 117849423, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=117849423]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":117849423,"title":"Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products","translated_title":"","metadata":{"abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. 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Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","internal_url":"https://www.academia.edu/117849423/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_internal_url":"","created_at":"2024-04-21T19:59:39.780-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":48736299,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":48736299,"first_name":"Ruzainah","middle_initials":null,"last_name":"Ali","page_name":"RuzainahAli","domain_name":"independent","created_at":"2016-05-15T00:49:30.834-07:00","display_name":"Ruzainah Ali","url":"https://independent.academia.edu/RuzainahAli"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":27756,"name":"DNA Barcoding","url":"https://www.academia.edu/Documents/in/DNA_Barcoding"}],"urls":[{"id":41287020,"url":"https://doi.org/10.1016/j.matpr.2020.12.131"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94149034"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94149034/EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online"><img alt="Research paper thumbnail of EASIFRENCH: A Holistic Approach In Teaching Language Online" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94149034/EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online">EASIFRENCH: A Holistic Approach In Teaching Language Online</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This research was conducted to develop and evaluate a prototype of Web-based Basic French coursew...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This research was conducted to develop and evaluate a prototype of Web-based Basic French courseware (multimedia courseware) for Basic French subject at diploma level. It also engaged of web applications that offer various facilities in providing interactive, effective and innovative learning environment. This research also evaluates some features of Basic French courseware such as pedagogical approach, learning strategies, theories in education and effectiveness usage test in term of achievement towards the application of Basic French courseware in teaching and learning compared to conventional way. The research methodology applied was pra and post quasi-experimental for balanced groups. The samples for the study were 4 Subject Experts, 56 students and 3 lecturers from UniKL Malaysia France Institute (UniKL MFI). The students were divided into two groups where 28 students use the Web-based Basic French courseware (treatment group) and 28 students learn Basic French in conventional ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94149034"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94149034"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94149034; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94149034]").text(description); $(".js-view-count[data-work-id=94149034]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94149034; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94149034']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94149034, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=94149034]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94149034,"title":"EASIFRENCH: A Holistic Approach In Teaching Language Online","translated_title":"","metadata":{"abstract":"This research was conducted to develop and evaluate a prototype of Web-based Basic French courseware (multimedia courseware) for Basic French subject at diploma level. 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The samples for the study were 4 Subject Experts, 56 students and 3 lecturers from UniKL Malaysia France Institute (UniKL MFI). The students were divided into two groups where 28 students use the Web-based Basic French courseware (treatment group) and 28 students learn Basic French in conventional ...","internal_url":"https://www.academia.edu/94149034/EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online","translated_internal_url":"","created_at":"2023-01-02T02:53:06.197-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":48736299,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":48736299,"first_name":"Ruzainah","middle_initials":null,"last_name":"Ali","page_name":"RuzainahAli","domain_name":"independent","created_at":"2016-05-15T00:49:30.834-07:00","display_name":"Ruzainah Ali","url":"https://independent.academia.edu/RuzainahAli"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94148982"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94148982/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products"><img alt="Research paper thumbnail of Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94148982/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products">Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products</a></div><div class="wp-workCard_item"><span>Materials Today: Proceedings</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short D...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94148982"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94148982"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94148982; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94148982]").text(description); $(".js-view-count[data-work-id=94148982]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94148982; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94148982']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94148982, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=94148982]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94148982,"title":"Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products","translated_title":"","metadata":{"abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Materials Today: Proceedings"},"translated_abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","internal_url":"https://www.academia.edu/94148982/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_internal_url":"","created_at":"2023-01-02T02:52:35.007-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":48736299,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":48736299,"first_name":"Ruzainah","middle_initials":null,"last_name":"Ali","page_name":"RuzainahAli","domain_name":"independent","created_at":"2016-05-15T00:49:30.834-07:00","display_name":"Ruzainah Ali","url":"https://independent.academia.edu/RuzainahAli"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":27756,"name":"DNA Barcoding","url":"https://www.academia.edu/Documents/in/DNA_Barcoding"}],"urls":[{"id":27654365,"url":"https://api.elsevier.com/content/article/PII:S2214785320398163?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="7426600"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/7426600/The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014"><img alt="Research paper thumbnail of The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014." class="work-thumbnail" src="https://attachments.academia-assets.com/34754519/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/7426600/The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014">The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014.</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://unikl.academia.edu/AssocProfTsDrKamaruzzamanIsmail">Assoc. Prof. Ts. Dr. Kamaruzzaman Ismail</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RuzainahAli">Ruzainah Ali</a></span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b1aefb747e2f1f5ca2b6797b2cfe3242" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":34754519,"asset_id":7426600,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/34754519/download_file?st=MTczMjcwMzM1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="7426600"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="7426600"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 7426600; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=7426600]").text(description); $(".js-view-count[data-work-id=7426600]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 7426600; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='7426600']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 7426600, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b1aefb747e2f1f5ca2b6797b2cfe3242" } } $('.js-work-strip[data-work-id=7426600]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":7426600,"title":"The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014.","translated_title":"","metadata":{"grobid_abstract":"This study aims to describe the pros and cons of social networking sites. Transforming the conventional media to new media was planned change people's minds. The explosion of information that can be accessed by all Malaysians through wireless broadband has a major change in the political scenario Malaysia. Social media instruments and applications are tools that people use to share information with their network, which may include family, friends and co-workers, among many other people. Obviously, the use of the Internet has exponentially increased as the largest social media tool as time has passed and thousands of tools have become available through it. This is evidenced by a general election reforms to its 13 th General Election where Front National (BN) using the mainstream media and opposition parties other alternatives to using new media to campaign. As a result, 48% of people voted for the Front National, while 52 % favour to Pakatan Rakyat. The percentage of the popular vote is proving how social media can change the landscape thoughts and thinking patterns of someone on a particular issue or subject. The study used literature reviews and interviews with several individuals. The results showed that social networking sites have great influence in the direction of an organization or a country based on the pros and cons that are in use. The study also aims to share information on few social networking sites that can help in teaching and learning. This is particularly important for ensuring that instructors use different styles or methodology and address the issue of Y-generation in accordance with the abrupt change in the era of information and communication technology.","grobid_abstract_attachment_id":34754519},"translated_abstract":null,"internal_url":"https://www.academia.edu/7426600/The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014","translated_internal_url":"","created_at":"2014-06-22T12:33:45.968-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":13183022,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4504752,"work_id":7426600,"tagging_user_id":13183022,"tagged_user_id":null,"co_author_invite_id":1043018,"email":"j***l@unikl.edu.my","display_order":0,"name":"D. Othman","title":"The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014."},{"id":20153667,"work_id":7426600,"tagging_user_id":13183022,"tagged_user_id":48736299,"co_author_invite_id":1043075,"email":"a***h@gmail.com","display_order":4194304,"name":"Ruzainah Ali","title":"The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014."}],"downloadable_attachments":[{"id":34754519,"title":"","file_type":"docx","scribd_thumbnail_url":"https://attachments.academia-assets.com/34754519/thumbnails/1.jpg","file_name":"Full_Paper_THE_INFLUENCE_OF_SOCIAL_MEDIA_ON_SOCIETY_AND_ITS_APPLICATION_ICET2.docx","download_url":"https://www.academia.edu/attachments/34754519/download_file?st=MTczMjcwMzM1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_Influence_Of_Social_Media_on_Society.docx","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/34754519/Full_Paper_THE_INFLUENCE_OF_SOCIAL_MEDIA_ON_SOCIETY_AND_ITS_APPLICATION_ICET2.docx?1410893514=\u0026response-content-disposition=attachment%3B+filename%3DThe_Influence_Of_Social_Media_on_Society.docx\u0026Expires=1732706953\u0026Signature=Fh-dnE77yFzxh2zeNOXfGWxvGgfqMc9eYQFoKXiW~UAUZR21so6~0sp9-brXP~YsroSbyprk6aAiom4w7xgpFDp4DGeKOjyueswV0R~M6QXuD9jmM56kKiED0KH8SPBdyo4nLBxFoFQO1u4rNCWLMgssRMH9mkxtn37QfrL1HsimPGzAToDfBWeKB24dglzK64UB7ukrElTD-GdV2YJcsuAui2Eqi3Ey-2BNukFHOhUpr96Nuv3lVLWTZd09OuzPwcyTTMrtvPXqQV8tTCx~QQHBfl4v1EYFrZc~namb~MlTd8Bh0K-ZygLyQSDWwBlvaG8lKqnN5AAiJEb9A1WzjQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":13183022,"first_name":"Assoc. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="5219585" id="papers"><div class="js-work-strip profile--work_container" data-work-id="122564249"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122564249/Essential_Oils_from_Traditional_Medicinal_Plants_in_Malaysia_A_Review_on_Their_Traditional_Uses_Bioactive_Constituents_and_Therapeutic_Potential"><img alt="Research paper thumbnail of Essential Oils from Traditional Medicinal Plants in Malaysia: A Review on Their Traditional Uses, Bioactive Constituents and Therapeutic Potential" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122564249/Essential_Oils_from_Traditional_Medicinal_Plants_in_Malaysia_A_Review_on_Their_Traditional_Uses_Bioactive_Constituents_and_Therapeutic_Potential">Essential Oils from Traditional Medicinal Plants in Malaysia: A Review on Their Traditional Uses, Bioactive Constituents and Therapeutic Potential</a></div><div class="wp-workCard_item"><span>Pharmaceutical Chemistry Journal</span><span>, Sep 15, 2023</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122564249"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122564249"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122564249; 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Boerl. and its processed commercial products" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/117849423/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products">Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products</a></div><div class="wp-workCard_item"><span>Materials Today: Proceedings</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short D...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="117849423"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="117849423"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 117849423; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=117849423]").text(description); $(".js-view-count[data-work-id=117849423]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 117849423; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='117849423']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 117849423, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=117849423]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":117849423,"title":"Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products","translated_title":"","metadata":{"abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Materials Today: Proceedings"},"translated_abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","internal_url":"https://www.academia.edu/117849423/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_internal_url":"","created_at":"2024-04-21T19:59:39.780-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":48736299,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":48736299,"first_name":"Ruzainah","middle_initials":null,"last_name":"Ali","page_name":"RuzainahAli","domain_name":"independent","created_at":"2016-05-15T00:49:30.834-07:00","display_name":"Ruzainah Ali","url":"https://independent.academia.edu/RuzainahAli"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":27756,"name":"DNA Barcoding","url":"https://www.academia.edu/Documents/in/DNA_Barcoding"}],"urls":[{"id":41287020,"url":"https://doi.org/10.1016/j.matpr.2020.12.131"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94149034"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94149034/EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online"><img alt="Research paper thumbnail of EASIFRENCH: A Holistic Approach In Teaching Language Online" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94149034/EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online">EASIFRENCH: A Holistic Approach In Teaching Language Online</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This research was conducted to develop and evaluate a prototype of Web-based Basic French coursew...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This research was conducted to develop and evaluate a prototype of Web-based Basic French courseware (multimedia courseware) for Basic French subject at diploma level. It also engaged of web applications that offer various facilities in providing interactive, effective and innovative learning environment. This research also evaluates some features of Basic French courseware such as pedagogical approach, learning strategies, theories in education and effectiveness usage test in term of achievement towards the application of Basic French courseware in teaching and learning compared to conventional way. The research methodology applied was pra and post quasi-experimental for balanced groups. The samples for the study were 4 Subject Experts, 56 students and 3 lecturers from UniKL Malaysia France Institute (UniKL MFI). The students were divided into two groups where 28 students use the Web-based Basic French courseware (treatment group) and 28 students learn Basic French in conventional ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94149034"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94149034"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94149034; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94149034]").text(description); $(".js-view-count[data-work-id=94149034]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94149034; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94149034']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94149034, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=94149034]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94149034,"title":"EASIFRENCH: A Holistic Approach In Teaching Language Online","translated_title":"","metadata":{"abstract":"This research was conducted to develop and evaluate a prototype of Web-based Basic French courseware (multimedia courseware) for Basic French subject at diploma level. It also engaged of web applications that offer various facilities in providing interactive, effective and innovative learning environment. This research also evaluates some features of Basic French courseware such as pedagogical approach, learning strategies, theories in education and effectiveness usage test in term of achievement towards the application of Basic French courseware in teaching and learning compared to conventional way. The research methodology applied was pra and post quasi-experimental for balanced groups. The samples for the study were 4 Subject Experts, 56 students and 3 lecturers from UniKL Malaysia France Institute (UniKL MFI). The students were divided into two groups where 28 students use the Web-based Basic French courseware (treatment group) and 28 students learn Basic French in conventional ..."},"translated_abstract":"This research was conducted to develop and evaluate a prototype of Web-based Basic French courseware (multimedia courseware) for Basic French subject at diploma level. It also engaged of web applications that offer various facilities in providing interactive, effective and innovative learning environment. This research also evaluates some features of Basic French courseware such as pedagogical approach, learning strategies, theories in education and effectiveness usage test in term of achievement towards the application of Basic French courseware in teaching and learning compared to conventional way. The research methodology applied was pra and post quasi-experimental for balanced groups. The samples for the study were 4 Subject Experts, 56 students and 3 lecturers from UniKL Malaysia France Institute (UniKL MFI). The students were divided into two groups where 28 students use the Web-based Basic French courseware (treatment group) and 28 students learn Basic French in conventional ...","internal_url":"https://www.academia.edu/94149034/EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online","translated_internal_url":"","created_at":"2023-01-02T02:53:06.197-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":48736299,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"EASIFRENCH_A_Holistic_Approach_In_Teaching_Language_Online","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":48736299,"first_name":"Ruzainah","middle_initials":null,"last_name":"Ali","page_name":"RuzainahAli","domain_name":"independent","created_at":"2016-05-15T00:49:30.834-07:00","display_name":"Ruzainah Ali","url":"https://independent.academia.edu/RuzainahAli"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94148982"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94148982/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products"><img alt="Research paper thumbnail of Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94148982/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products">Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products</a></div><div class="wp-workCard_item"><span>Materials Today: Proceedings</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short D...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94148982"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94148982"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94148982; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94148982]").text(description); $(".js-view-count[data-work-id=94148982]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94148982; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94148982']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94148982, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=94148982]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94148982,"title":"Application of rbcL in DNA barcoding of Phaleria macrocarpa (Scheff.) Boerl. and its processed commercial products","translated_title":"","metadata":{"abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Materials Today: Proceedings"},"translated_abstract":"Abstract DNA barcoding is a molecular-based identification method which utilizes specific short DNA fragment known as DNA barcode. It can be used to assist species identification of specimens already lacking its original morphological traits such as processed herbal products. Phaleria macrocarpa (Scheff.) Boerl. is a plant species that originated from Malaysia and Indonesia and is locally known as God’s crown or Mahkota dewa in Malaysia. It is frequently used in Malaysian and Indonesian traditional medicines. Its fruits for example are used to treat flu, rheumatism, cancer, diabetes mellitus and hypertension. Due to its restorative properties, this plant is now commercially grown in Malaysia where its fruit is usually processed into various types of herbal products such as capsules, tea and coffee. However, due to the increasing popularity of this plant, it is important to have an effective method that can determine the authenticity of P. macrocarpa based products. In this study, the plastid rbcL barcoding gene was used to verify the authenticity of two local processed products claimed to have been made using the fruit of P. macrocarpa. The amplification of the partial rbcL gene was done via Polymerase Chain Reaction (PCR) using KAPA3G Plant PCR Kit. The DNA sequencing results were trimmed using ChromasPro before being aligned using ClustalW which was included in MEGA5. Species verification using the BLASTn search tool revealed that the species contained in both products were found to be P. macrocarpa. Phylogenetic reconstruction via Neighbour-Joining tree using partial rbcL gene amplified from the two tested products and fresh Mahkota dewa plant also revealed high similarity, supporting the BLASTn analysis. In conclusion, this study has shown that the rbcL barcode is suitable to be used to determine the authenticity of processed commercial products of P. macrocarpa. Furthermore, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products. To the extent of our abilities, we believe that there is currently no DNA barcoding study that focuses solely on P. macrocarpa and its commercial products, using the KAPA3G Plant PCR kit. We propose that to determine the authenticity of any P. macrcarpa based products; the rbcL barcode can be used with the KAPA3G Plant PCR kit, especially in laboratories that do not have access to liquid nitrogen.","internal_url":"https://www.academia.edu/94148982/Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_internal_url":"","created_at":"2023-01-02T02:52:35.007-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":48736299,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Application_of_rbcL_in_DNA_barcoding_of_Phaleria_macrocarpa_Scheff_Boerl_and_its_processed_commercial_products","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":48736299,"first_name":"Ruzainah","middle_initials":null,"last_name":"Ali","page_name":"RuzainahAli","domain_name":"independent","created_at":"2016-05-15T00:49:30.834-07:00","display_name":"Ruzainah Ali","url":"https://independent.academia.edu/RuzainahAli"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":27756,"name":"DNA Barcoding","url":"https://www.academia.edu/Documents/in/DNA_Barcoding"}],"urls":[{"id":27654365,"url":"https://api.elsevier.com/content/article/PII:S2214785320398163?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="7426600"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/7426600/The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014"><img alt="Research paper thumbnail of The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014." class="work-thumbnail" src="https://attachments.academia-assets.com/34754519/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/7426600/The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014">The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014.</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://unikl.academia.edu/AssocProfTsDrKamaruzzamanIsmail">Assoc. Prof. Ts. Dr. Kamaruzzaman Ismail</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RuzainahAli">Ruzainah Ali</a></span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b1aefb747e2f1f5ca2b6797b2cfe3242" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":34754519,"asset_id":7426600,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/34754519/download_file?st=MTczMjcwMzM1Myw4LjIyMi4yMDguMTQ2&st=MTczMjcwMzM1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="7426600"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="7426600"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 7426600; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=7426600]").text(description); $(".js-view-count[data-work-id=7426600]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 7426600; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='7426600']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 7426600, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b1aefb747e2f1f5ca2b6797b2cfe3242" } } $('.js-work-strip[data-work-id=7426600]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":7426600,"title":"The Influence Of Social Media on Society and Its Application in Education. International Conference on Engineering Technology (ICE2T). Berjaya Times Square Hotel. 27-29th August 2014.","translated_title":"","metadata":{"grobid_abstract":"This study aims to describe the pros and cons of social networking sites. Transforming the conventional media to new media was planned change people's minds. The explosion of information that can be accessed by all Malaysians through wireless broadband has a major change in the political scenario Malaysia. Social media instruments and applications are tools that people use to share information with their network, which may include family, friends and co-workers, among many other people. Obviously, the use of the Internet has exponentially increased as the largest social media tool as time has passed and thousands of tools have become available through it. This is evidenced by a general election reforms to its 13 th General Election where Front National (BN) using the mainstream media and opposition parties other alternatives to using new media to campaign. As a result, 48% of people voted for the Front National, while 52 % favour to Pakatan Rakyat. The percentage of the popular vote is proving how social media can change the landscape thoughts and thinking patterns of someone on a particular issue or subject. The study used literature reviews and interviews with several individuals. The results showed that social networking sites have great influence in the direction of an organization or a country based on the pros and cons that are in use. The study also aims to share information on few social networking sites that can help in teaching and learning. This is particularly important for ensuring that instructors use different styles or methodology and address the issue of Y-generation in accordance with the abrupt change in the era of information and communication technology.","grobid_abstract_attachment_id":34754519},"translated_abstract":null,"internal_url":"https://www.academia.edu/7426600/The_Influence_Of_Social_Media_on_Society_and_Its_Application_in_Education_International_Conference_on_Engineering_Technology_ICE2T_Berjaya_Times_Square_Hotel_27_29th_August_2014","translated_internal_url":"","created_at":"2014-06-22T12:33:45.968-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":13183022,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4504752,"work_id":7426600,"tagging_user_id":13183022,"tagged_user_id":null,"co_author_invite_id":1043018,"email":"j***l@unikl.edu.my","display_order":0,"name":"D. 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