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Goustin</h1><div class="affiliations-container fake-truncate js-profile-affiliations"><div><a class="u-tcGrayDarker" href="https://wayne.academia.edu/">Wayne State University</a>, <a class="u-tcGrayDarker" href="https://wayne.academia.edu/Departments/Center_for_Molecular_Medicine_and_Genetics/Documents">Center for Molecular Medicine and Genetics</a>, <span class="u-tcGrayDarker">Post-Doc</span></div></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="A." data-follow-user-id="34163772" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" 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class="data"></p></div></a></div><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="suggested-academics-container"><div class="suggested-academics--header"><p class="ds2-5-body-md-bold">Related Authors</p></div><ul class="suggested-user-card-list"><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://independent.academia.edu/FredWilt"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/FredWilt">Fred Wilt</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://wayne.academia.edu/AntonScottGoustin"><img class="profile-avatar u-positionAbsolute" alt="Anton-Scott Goustin" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" src="https://gravatar.com/avatar/ca827993182c203ad242287bc140c312?s=200" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://wayne.academia.edu/AntonScottGoustin">Anton-Scott Goustin</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Wayne State University</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://independent.academia.edu/HughWoodland"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/HughWoodland">Hugh Woodland</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://independent.academia.edu/AlbertoMonroy"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/AlbertoMonroy">Alberto Monroy</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://sfu.academia.edu/BBrandhorst"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://sfu.academia.edu/BBrandhorst">Bruce P Brandhorst</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Simon Fraser University</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://independent.academia.edu/MerrillHille"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/MerrillHille">Merrill Hille</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://independent.academia.edu/patrickCormier1"><img class="profile-avatar u-positionAbsolute" alt="patrick Cormier" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/64976728/46813949/36060534/s200_patrick.cormier.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/patrickCormier1">patrick Cormier</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://umd.academia.edu/WilliamJeffery"><img class="profile-avatar u-positionAbsolute" alt="William Jeffery" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/35260705/10241620/11429720/s200_william.jeffery.jpg_oh_f0eac5f4b2d8de93ec055a3189ef1aa6_oe_56a1bdde" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://umd.academia.edu/WilliamJeffery">William Jeffery</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">University of Maryland</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://independent.academia.edu/JuliaMorales7"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/JuliaMorales7">Julia Morales</a></div></div><div class="suggested-user-card"><div 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data-component-name="Pill" data-props="{"color":"gray","children":["Gene expression"]}" data-trace="false" data-dom-id="Pill-react-component-5ad9c3cb-7e26-49a0-a42e-1df9d647861b"></div> <div id="Pill-react-component-5ad9c3cb-7e26-49a0-a42e-1df9d647861b"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="34163772" href="https://www.academia.edu/Documents/in/Epigenetics"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Epigenetics"]}" data-trace="false" data-dom-id="Pill-react-component-f4355f3c-c3cd-4c42-a00d-6df6fa03e205"></div> <div id="Pill-react-component-f4355f3c-c3cd-4c42-a00d-6df6fa03e205"></div> </a></div></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="nav-container backbone-profile-documents-nav hidden-xs"><ul class="nav-tablist" role="tablist"><li class="nav-chip active" role="presentation"><a data-section-name="" data-toggle="tab" href="#all" role="tab">all</a></li><li class="nav-chip" role="presentation"><a class="js-profile-docs-nav-section u-textTruncate" data-click-track="profile-works-tab" data-section-name="Books" data-toggle="tab" href="#books" role="tab" title="Books"><span>1</span> <span class="ds2-5-body-sm-bold">Books</span></a></li><li class="nav-chip" role="presentation"><a class="js-profile-docs-nav-section u-textTruncate" data-click-track="profile-works-tab" data-section-name="Papers" data-toggle="tab" href="#papers" role="tab" title="Papers"><span>38</span> <span class="ds2-5-body-sm-bold">Papers</span></a></li></ul></div><div class="divider ds-divider-16" style="margin: 0px;"></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Books" id="Books"><h3 class="profile--tab_heading_container">Books by A. Goustin</h3></div><div class="js-work-strip profile--work_container" data-work-id="6471532"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/6471532/Regulation_of_Cytochrome_c_in_Respiration_Apoptosis_Neurodegeneration_and_Cancer_The_Good_the_Bad_and_the_Ugly"><img alt="Research paper thumbnail of Regulation of Cytochrome c in Respiration, Apoptosis, Neurodegeneration and Cancer: The Good, the Bad and the Ugly " class="work-thumbnail" src="https://attachments.academia-assets.com/33258566/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/6471532/Regulation_of_Cytochrome_c_in_Respiration_Apoptosis_Neurodegeneration_and_Cancer_The_Good_the_Bad_and_the_Ugly">Regulation of Cytochrome c in Respiration, Apoptosis, Neurodegeneration and Cancer: The Good, the Bad and the Ugly </a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://michiganstate.academia.edu/HosamElbaz">Hosam A Elbaz</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://wayne.academia.edu/AGoustin">A. Goustin</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://wayne.academia.edu/ChristopherSinkler">Christopher Sinkler</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LeeSennottmiller">Lee Sennott-miller</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JLiu13">J. Liu</a></span></div><div class="wp-workCard_item"><span>Cytochromes b and c: Biochemical Properties, Biological Functions and Electrochemical Analysis </span><span>, Mar 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Despite the fact that over 200 phosphorylation sites have been mapped on the mitochondrial oxidat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Despite the fact that over 200 phosphorylation sites have been mapped on the mitochondrial oxidative phosphorylation (OxPhos) complexes, very little is known about the relevant cell signaling pathways and the terminal kinases and phosphatases that control these phosphorylations. Within OxPhos, cytochrome c (Cytc) plays a special role because it is not only involved in electron transport but is also a key executer of apoptosis when it is released from the mitochondria. It is therefore not surprising that Cytc is regulated by phosphorylation. Four phosphorylation sites have been mapped on mammalian Cytc, two of which have been studied functionally, demonstrating that both respiration and apoptosis are under the control of signaling pathways that have yet to be identified. We here review the regulation and multiple functions of mammalian Cytc, including respiration, reactive oxygen species (ROS) scavenging under healthy conditions, ROS production via p66Shc, and cardiolipin oxidation during apoptosis. We propose targeting Cytc by manipulation of signaling cascades as a therapeutic avenue in conditions including neurodegeneration and cancer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f49a4e63648d89b94b1378082e4fe40d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":33258566,"asset_id":6471532,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/33258566/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="6471532"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="6471532"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6471532; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=6471532]").text(description); $(".js-view-count[data-work-id=6471532]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 6471532; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='6471532']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f49a4e63648d89b94b1378082e4fe40d" } } $('.js-work-strip[data-work-id=6471532]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":6471532,"title":"Regulation of Cytochrome c in Respiration, Apoptosis, Neurodegeneration and Cancer: The Good, the Bad and the Ugly ","internal_url":"https://www.academia.edu/6471532/Regulation_of_Cytochrome_c_in_Respiration_Apoptosis_Neurodegeneration_and_Cancer_The_Good_the_Bad_and_the_Ugly","owner_id":2723771,"coauthors_can_edit":true,"owner":{"id":2723771,"first_name":"Hosam","middle_initials":"A","last_name":"Elbaz","page_name":"HosamElbaz","domain_name":"michiganstate","created_at":"2012-11-04T08:26:40.210-08:00","display_name":"Hosam A Elbaz","url":"https://michiganstate.academia.edu/HosamElbaz"},"attachments":[{"id":33258566,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/33258566/thumbnails/1.jpg","file_name":"Huttemann_Cytochromes_b_and_c_2014_printable.pdf","download_url":"https://www.academia.edu/attachments/33258566/download_file","bulk_download_file_name":"Regulation_of_Cytochrome_c_in_Respiratio.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/33258566/Huttemann_Cytochromes_b_and_c_2014_printable-libre.pdf?1395227011=\u0026response-content-disposition=attachment%3B+filename%3DRegulation_of_Cytochrome_c_in_Respiratio.pdf\u0026Expires=1740946884\u0026Signature=ZByrxCjPXYj7e19Zy65T~BzHi~Mhg2uAwesjgf2Z-9LFbXiRgvMNjyDBF8d94eQf37WaSjir5pkAB0BVIJ4~xz-ltEWoKAoBTEYtsZQOw~vtarOScpsxEY941HyOQA6pdr4fM3FlN2H~s5EkxmZn7RvGLLWnovPM9ndrORWVjeKcrg5cwTAmX5dWOSm~75j-MYcEHcsPiLT3-7LIuLlGRhnU71-MXaIoP0aA0PV0Prc8Gdw~-1NXiIANPBuCAIcgtnRnGLf9zX8ffyPbAenmJG9ip7W7kjx8f2KqLWOtI~SXAHgMRoSGqakQkY-Cz0QtnxNtjD-Z8bKK5jRvzRkajQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by A. Goustin</h3></div><div class="js-work-strip profile--work_container" data-work-id="47674462"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/47674462/Protein_synthesis_polyribosomes_and_peptide_elongation_in_early_development_of_Strongylocentrotus_purpuratus"><img alt="Research paper thumbnail of Protein synthesis, polyribosomes, and peptide elongation in early development of Strongylocentrotus purpuratus" class="work-thumbnail" src="https://attachments.academia-assets.com/66644681/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/47674462/Protein_synthesis_polyribosomes_and_peptide_elongation_in_early_development_of_Strongylocentrotus_purpuratus">Protein synthesis, polyribosomes, and peptide elongation in early development of Strongylocentrotus purpuratus</a></div><div class="wp-workCard_item"><span>Developmental Biology</span><span>, 1981</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The absolute rate of protein synthesis in developing embryos of Strm&wentrotvs purpwatw has been ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The absolute rate of protein synthesis in developing embryos of Strm&wentrotvs purpwatw has been measured by lysine incorporation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ad17207400022136d4095c3d725b8b39" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":66644681,"asset_id":47674462,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/66644681/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="47674462"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="47674462"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 47674462; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=47674462]").text(description); $(".js-view-count[data-work-id=47674462]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 47674462; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='47674462']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ad17207400022136d4095c3d725b8b39" } } $('.js-work-strip[data-work-id=47674462]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":47674462,"title":"Protein synthesis, polyribosomes, and peptide elongation in early development of Strongylocentrotus purpuratus","internal_url":"https://www.academia.edu/47674462/Protein_synthesis_polyribosomes_and_peptide_elongation_in_early_development_of_Strongylocentrotus_purpuratus","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":66644681,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/66644681/thumbnails/1.jpg","file_name":"0012-1606_2881_2990426-720210423-25423-12x6vrv.pdf","download_url":"https://www.academia.edu/attachments/66644681/download_file","bulk_download_file_name":"Protein_synthesis_polyribosomes_and_pept.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/66644681/0012-1606_2881_2990426-720210423-25423-12x6vrv-libre.pdf?1619235141=\u0026response-content-disposition=attachment%3B+filename%3DProtein_synthesis_polyribosomes_and_pept.pdf\u0026Expires=1740946884\u0026Signature=IU~zUn65Xg59mxFBRLmdDhBqGTNKIcokYFRVUmodPeaUciTZAmvVY8cC1ssHkxQhYCpVdCba3Mju39-Yiw2VPI4jgm7MgXitniQqvBJ5LTruYjR65KQePJH~YV26X-wNh2l4H3Woo8mJ~jwCJk9aeuuM6V6FdjUzf~nOyo6uCJ2aU2ZzupsU-OaaW5LKCgVzrst3QTjA5ab7zwyACyEkPL7ToHOqCwyStBjDTCvLEPgNXE5a06NFqG2p9G7hVkBYsuu1o-~Wl9kUiyX~uBLAFojEdZkPJaOawV9xXhKB25QtFX5bU-5FAgTb966d9-sKOiAyPFwL~uyuHvN81ch3cw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328277"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328277/Elongation_of_teichuronic_acid_chains_by_a_wall_membrane_preparation_from_Micrococcus_luteus"><img alt="Research paper thumbnail of Elongation of teichuronic acid chains by a wall-membrane preparation from Micrococcus luteus" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328277/Elongation_of_teichuronic_acid_chains_by_a_wall_membrane_preparation_from_Micrococcus_luteus">Elongation of teichuronic acid chains by a wall-membrane preparation from Micrococcus luteus</a></div><div class="wp-workCard_item"><span>Journal of bacteriology</span><span>, 1982</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A wall-plus-membrane preparation from Micrococcus luteus catalyzes the incorporation of [14C]gluc...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A wall-plus-membrane preparation from Micrococcus luteus catalyzes the incorporation of [14C]glucose from UDP-[14C]glucose, into two fractions of teichuronic acid, which is the cell wall polysaccharide consisting of alternating residues of glucose and N-acetylmannosaminuronic acid (ManNAcUA). Membrane-associated teichuronic acid was extracted from the wall-membrane fraction of reaction mixtures by sodium dodecyl sulfate. The synthesis of membrane-associated teichuronic acid required UDP-glucose, UDP-ManNAcUA, and UDP-N-acetylglucosamine and was inhibited by tunicamycin. Glucose incorporated into wall-bound teichuronic acid remained in wall fragments after extraction with sodium dodecyl sulfate, and its incorporation required UDP-glucose and UDP-ManNAcUA (but not UDP-N-acetylglucosamine) and was insensitive to tunicamycin. Radioactive material incorporated into wall-bound teichuronic acid could be released by treatment with mild acid or by digestion with lysozyme, indicating that the...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328277"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328277"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328277; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328277]").text(description); $(".js-view-count[data-work-id=21328277]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328277; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328277']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328277]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328277,"title":"Elongation of teichuronic acid chains by a wall-membrane preparation from Micrococcus luteus","internal_url":"https://www.academia.edu/21328277/Elongation_of_teichuronic_acid_chains_by_a_wall_membrane_preparation_from_Micrococcus_luteus","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328276"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328276/Direct_measurement_of_histone_peptide_elongation_rate_in_cleaving_sea_urchin_embryos"><img alt="Research paper thumbnail of Direct measurement of histone peptide elongation rate in cleaving sea urchin embryos" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328276/Direct_measurement_of_histone_peptide_elongation_rate_in_cleaving_sea_urchin_embryos">Direct measurement of histone peptide elongation rate in cleaving sea urchin embryos</a></div><div class="wp-workCard_item"><span>Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression</span><span>, 1982</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Regulation of protein synthesis can be exercised at a number of levels. One of the more experimen...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Regulation of protein synthesis can be exercised at a number of levels. One of the more experimentally difficult levels to approach has been the measurement of peptide elongation rate. This paper presents a new application of the cyanogen bromide (CNBr) cleavage of proteins in a direct measurement of histone peptide elongation rate in cleaving sea urchin embryos (Strongylocentrotus purpuratus). The data indicate an elongation rate (at 15 degrees C) for histones H2B and H1 alpha of 0.69 and 0.80 codons per s, respectively. These values fall within the range of previously published values of average peptide elongation rate for total protein in these cells. This method should be generally applicable to many systems for which the measurement of peptide elongation rate may provide a key to the understanding of the regulation of protein synthesis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328276"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328276"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328276; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328276]").text(description); $(".js-view-count[data-work-id=21328276]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328276; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328276']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328276]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328276,"title":"Direct measurement of histone peptide elongation rate in cleaving sea urchin embryos","internal_url":"https://www.academia.edu/21328276/Direct_measurement_of_histone_peptide_elongation_rate_in_cleaving_sea_urchin_embryos","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328271"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328271/Spread_of_HIV_type_1_in_Slovakia_remains_limited_and_is_restricted_to_subtype_B"><img alt="Research paper thumbnail of Spread of HIV type 1 in Slovakia remains limited and is restricted to subtype B" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328271/Spread_of_HIV_type_1_in_Slovakia_remains_limited_and_is_restricted_to_subtype_B">Spread of HIV type 1 in Slovakia remains limited and is restricted to subtype B</a></div><div class="wp-workCard_item"><span>AIDS research and human retroviruses</span><span>, Jan 20, 1996</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328271"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328271"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328271; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328271]").text(description); $(".js-view-count[data-work-id=21328271]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328271; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328271']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328271]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328271,"title":"Spread of HIV type 1 in Slovakia remains limited and is restricted to subtype B","internal_url":"https://www.academia.edu/21328271/Spread_of_HIV_type_1_in_Slovakia_remains_limited_and_is_restricted_to_subtype_B","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328270"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328270/Recombinant_human_alpha_2_HS_glycoprotein_inhibits_insulin_stimulated_mitogenic_pathway_without_affecting_metabolic_signalling_in_Chinese_hamster_ovary_cells_overexpressing_the_human_insulin_receptor"><img alt="Research paper thumbnail of Recombinant human alpha 2-HS glycoprotein inhibits insulin-stimulated mitogenic pathway without affecting metabolic signalling in Chinese hamster ovary cells overexpressing the human insulin receptor" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328270/Recombinant_human_alpha_2_HS_glycoprotein_inhibits_insulin_stimulated_mitogenic_pathway_without_affecting_metabolic_signalling_in_Chinese_hamster_ovary_cells_overexpressing_the_human_insulin_receptor">Recombinant human alpha 2-HS glycoprotein inhibits insulin-stimulated mitogenic pathway without affecting metabolic signalling in Chinese hamster ovary cells overexpressing the human insulin receptor</a></div><div class="wp-workCard_item"><span>Cellular signalling</span><span>, 1996</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Insulin acts on its target tissues by specific interaction with the cell surface insulin receptor...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Insulin acts on its target tissues by specific interaction with the cell surface insulin receptor (IR). The IR possesses an intrinsic tyrosine kinase (TK) activity which is stimulated by insulin binding. This TK activity is required for many aspects of insulin signalling. We had earlier reported that human plasma alpha 2-HS glycoprotein (alpha 2-HSG) inhibits insulin-stimulated mitogenesis at the level of IR-TK (Mol Endo 7: 1445-1455, 1993). In the present study, using recombinant alpha 2-HSG, which possesses 50-100 times the specific activity of plasma alpha 2-HSG, we have further investigated the molecular basis of this effect. We examined the insulin-stimulated Ras signalling pathway in Chinese Hamster Ovary cells overexpressing the human IR. alpha 2-HSG inhibits insulin-induced tyrosine phosphorylation of IRS-1 and the subsequent association of GRB2, as well as Sos, with IRS-1. This inhibition results in reduced guanine nucleotide exchange in p21ras. alpha 2-HSG also inhibits th...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328270"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328270"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328270; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328270]").text(description); $(".js-view-count[data-work-id=21328270]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328270; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328270']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328270]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328270,"title":"Recombinant human alpha 2-HS glycoprotein inhibits insulin-stimulated mitogenic pathway without affecting metabolic signalling in Chinese hamster ovary cells overexpressing the human insulin receptor","internal_url":"https://www.academia.edu/21328270/Recombinant_human_alpha_2_HS_glycoprotein_inhibits_insulin_stimulated_mitogenic_pathway_without_affecting_metabolic_signalling_in_Chinese_hamster_ovary_cells_overexpressing_the_human_insulin_receptor","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328269"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328269/Alpha2_HSG_a_specific_inhibitor_of_insulin_receptor_autophosphorylation_interacts_with_the_insulin_receptor"><img alt="Research paper thumbnail of Alpha2-HSG, a specific inhibitor of insulin receptor autophosphorylation, interacts with the insulin receptor" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328269/Alpha2_HSG_a_specific_inhibitor_of_insulin_receptor_autophosphorylation_interacts_with_the_insulin_receptor">Alpha2-HSG, a specific inhibitor of insulin receptor autophosphorylation, interacts with the insulin receptor</a></div><div class="wp-workCard_item"><span>Molecular and cellular endocrinology</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human fetuin, [alpha2-Heremans Schmid Glycoprotein (alpha2-HSG)], is a natural inhibitor of insul...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human fetuin, [alpha2-Heremans Schmid Glycoprotein (alpha2-HSG)], is a natural inhibitor of insulin receptor tyrosine kinase activity (IR-TKA). Previously, we have demonstrated that alpha2-HSG inhibits the mitogenic pathway without affecting the metabolic arm of insulin signal transduction. In this study, we demonstrate the time-course and specificity of inhibition, its interaction with IR and probable physiological role. In intact rat1 fibroblasts overexpressing the human insulin receptor (HIRc B), incubation of recombinant human alpha2-HSGbac (1.8 microM) inhibited insulin-induced IR autophosphorylation by over 80%. This inhibitory effect of alpha2-HSGbac on insulin-induced IR autophosphorylation was blunted by half in 60 min. Interestingly, alpha2-HSGbac at similar concentrations (0.9 or 1.8 microM), had no effect on EGF- or IGF-I-induced cognate receptor autophosphorylation. Anti-alpha2-HSG immunoprecipitates of alpha2-HSGbac-treated HIRc B cell lysates demonstrated the presence...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328269"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328269"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328269; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328269]").text(description); $(".js-view-count[data-work-id=21328269]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328269; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328269']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328269]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328269,"title":"Alpha2-HSG, a specific inhibitor of insulin receptor autophosphorylation, interacts with the insulin receptor","internal_url":"https://www.academia.edu/21328269/Alpha2_HSG_a_specific_inhibitor_of_insulin_receptor_autophosphorylation_interacts_with_the_insulin_receptor","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328268"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328268/Genetic_Mapping_and_Functional_Studies_of_a_Natural_Inhibitor_of_the_Insulin_Receptor_Tyrosine_Kinase_The_Mouse_Ortholog_of_Human_%CE%B12_HS_Glycoprotein"><img alt="Research paper thumbnail of Genetic Mapping and Functional Studies of a Natural Inhibitor of the Insulin Receptor Tyrosine Kinase: The Mouse Ortholog of Human α2-HS Glycoprotein" class="work-thumbnail" src="https://attachments.academia-assets.com/41818095/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328268/Genetic_Mapping_and_Functional_Studies_of_a_Natural_Inhibitor_of_the_Insulin_Receptor_Tyrosine_Kinase_The_Mouse_Ortholog_of_Human_%CE%B12_HS_Glycoprotein">Genetic Mapping and Functional Studies of a Natural Inhibitor of the Insulin Receptor Tyrosine Kinase: The Mouse Ortholog of Human α2-HS Glycoprotein</a></div><div class="wp-workCard_item"><span>International Journal of Experimental Diabetes Research</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">IR-TKA in vitro. In addition, mouse o2-HSG (25tg/ml) completely abolishes insulin-induced DNA syn...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">IR-TKA in vitro. In addition, mouse o2-HSG (25tg/ml) completely abolishes insulin-induced DNA synthesis in H-35 rat hepatoma cells. Based on the sequence data and functional analysis, we conclude that the mouse Ahsg gene is the true ortholog of the human AHSG gene. 249 250 V.J. CINTRON et al. reactant protein and altered concentrations of c2-HSG have been reported in several disease conditions including Paget's disease, osteogenesis imperfecta, lymphoma, leukemia and myelofibrosis. 5, 61 Several functions have been attributed to c2-HSG, including its involvement in immune response, the chemotactic response of macrophages, 8, 91 enhancement of phagocytic function of human monocytes, bone mineralization, bone accumulation, calcification and as an inhibitor of insulin receptor tyrosine kinase activity [IR-TKA;18]. Recently, Jahnen-Dechent et al. demonstrated that mice deficient for c2-HSG can develop ectopic microcalcifications in soft tissues, supporting the idea that c2-HSG may operate as an inhibitor of apatite crystal growth in vivo.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="713931f805e3c4e8c33b288424ad79e4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818095,"asset_id":21328268,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818095/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328268"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328268"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328268; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328268]").text(description); $(".js-view-count[data-work-id=21328268]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328268; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328268']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "713931f805e3c4e8c33b288424ad79e4" } } $('.js-work-strip[data-work-id=21328268]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328268,"title":"Genetic Mapping and Functional Studies of a Natural Inhibitor of the Insulin Receptor Tyrosine Kinase: The Mouse Ortholog of Human α2-HS Glycoprotein","internal_url":"https://www.academia.edu/21328268/Genetic_Mapping_and_Functional_Studies_of_a_Natural_Inhibitor_of_the_Insulin_Receptor_Tyrosine_Kinase_The_Mouse_Ortholog_of_Human_%CE%B12_HS_Glycoprotein","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818095,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818095/thumbnails/1.jpg","file_name":"Genetic_Mapping_and_Functional_Studies_o20160131-9433-96wh78.pdf","download_url":"https://www.academia.edu/attachments/41818095/download_file","bulk_download_file_name":"Genetic_Mapping_and_Functional_Studies_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818095/Genetic_Mapping_and_Functional_Studies_o20160131-9433-96wh78-libre.pdf?1454277111=\u0026response-content-disposition=attachment%3B+filename%3DGenetic_Mapping_and_Functional_Studies_o.pdf\u0026Expires=1740946885\u0026Signature=BAVrAMaKojh0r5DDjXSX39QtFlOJfms5i-SDczkJUUDIeYxh3YYE~P9b8PL1v0FSRUn3-U7myaYMAnWWoM3csPtRLcjzJBtdZS-ElEklfA-7G-tRg~ZCxc5tj9hnwiQVUfWokhfZ4JT80C336mHelTwR3sf~0Z8zkmuCcz9a~xtTY8uCKEIMw9RtFxRx3GjU2QBzr0m2Vw33vqJFWB4DneOAaLPI0rYHZJkp~5uLeWCeFywEYMvW6h7pv~guuV6suq7zBBV1iOwCjeOfWw~H7pDYKfT7B6FpIn664Q37CMWQksarJQlIF04Rj5EwTlEbvv~Te~I9YcDlGTJ2LgS~vw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328267"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328267/Spatial_and_temporal_pattern_of_cellular_myc_oncogene_expression_in_developing_human_placenta_Implications_for_embryonic_cell_proliferation"><img alt="Research paper thumbnail of Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328267/Spatial_and_temporal_pattern_of_cellular_myc_oncogene_expression_in_developing_human_placenta_Implications_for_embryonic_cell_proliferation">Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation</a></div><div class="wp-workCard_item"><span>Cell</span><span>, 1984</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have analyzed staged human placentas by Northern, dot blot, and in situ hybridization to human...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have analyzed staged human placentas by Northern, dot blot, and in situ hybridization to human c-myc probes. Placental RNA exhibits a stage-specific appearance of a 2.4 kb transcript of the c-myc gene. The frequency of this transcript varies 20 to 30 fold over the course of placental development, showing a peak at 4-5 weeks after conception, where the myc transcripts comprise about 0.05% by weight of the total placental mRNA. A clear decline in placental c-myc transcription is seen before the end of the first trimester of pregnancy. In situ hybridization to 125I-labeled myc probes demonstrates an unequal spatial distribution of myc transcripts in placental with particularly high expression in the cytotrophoblastic shell of early placenta. Labeling of placental explants with 3H-thymidine, the localization of myc transcripts to cytotrophoblasts, and the temporal pattern of myc expression all support a strong correlation between myc transcript abundance and cytotrophoblast proliferation. We argue for a role for the c-myc gene in the proliferation of normal cells in this tissue.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328267"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328267"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328267; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328267]").text(description); $(".js-view-count[data-work-id=21328267]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328267; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328267']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328267]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328267,"title":"Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation","internal_url":"https://www.academia.edu/21328267/Spatial_and_temporal_pattern_of_cellular_myc_oncogene_expression_in_developing_human_placenta_Implications_for_embryonic_cell_proliferation","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328266"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328266/Sequence_Note_Genetic_Polymorphism_of_Envelope_V3_Region_of_HIV_Type_1_Subtypes_A_C_and_D_from_Nairobi_Kenya"><img alt="Research paper thumbnail of Sequence Note : Genetic Polymorphism of Envelope V3 Region of HIV Type 1 Subtypes A, C, and D from Nairobi, Kenya" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328266/Sequence_Note_Genetic_Polymorphism_of_Envelope_V3_Region_of_HIV_Type_1_Subtypes_A_C_and_D_from_Nairobi_Kenya">Sequence Note : Genetic Polymorphism of Envelope V3 Region of HIV Type 1 Subtypes A, C, and D from Nairobi, Kenya</a></div><div class="wp-workCard_item"><span>Aids Research and Human Retroviruses</span><span>, 1996</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">... J Virol 1995;69:263-271. 5. Ebbesen P, Hager H, Norskov-Lauritsen N, Aboagye-Mathiesen G, Zdr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... J Virol 1995;69:263-271. 5. Ebbesen P, Hager H, Norskov-Lauritsen N, Aboagye-Mathiesen G, Zdravkovic M, Villadsen J, Liu X, Petersen PM, Bambra C, Nyongo Page 4. 78 ZACHAR ET AL. A, Temmerman M, and Zachar V: Concurrence of high levels of in-</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328266"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328266"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328266; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328266]").text(description); $(".js-view-count[data-work-id=21328266]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328266; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328266']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328266]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328266,"title":"Sequence Note : Genetic Polymorphism of Envelope V3 Region of HIV Type 1 Subtypes A, C, and D from Nairobi, Kenya","internal_url":"https://www.academia.edu/21328266/Sequence_Note_Genetic_Polymorphism_of_Envelope_V3_Region_of_HIV_Type_1_Subtypes_A_C_and_D_from_Nairobi_Kenya","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328265"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328265/Coexpression_of_the_genes_for_platelet_derived_growth_factor_B_chain_receptor_and_macrophage_colony_stimulating_factor_1_receptor_during_monocytic_differentiation"><img alt="Research paper thumbnail of Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation" class="work-thumbnail" src="https://attachments.academia-assets.com/41818090/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328265/Coexpression_of_the_genes_for_platelet_derived_growth_factor_B_chain_receptor_and_macrophage_colony_stimulating_factor_1_receptor_during_monocytic_differentiation">Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Receptors for platelet-derived growth factor (PDGF) have not been identified previously to our kn...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Receptors for platelet-derived growth factor (PDGF) have not been identified previously to our knowledge in human myeloid cells that also produce PDGF. Here we report that phorbol ester-treated myeloid cells differentiated along the monocytic lineage express both a full-length 5.5kilobase (kb) mRNA and a predominant, truncated 4.6-kb mRNA coding for the PDGF B-chain receptor (PDGF-BR). PDGF-BR was identified in phorbol ester-differentiated myeloid cells by indirect immunofluorescence with an antibody specific to PDGF-BR. This anti-PDGF-BR was also used in immunoprecipitation studies to demonstrate that lysates of phorbol ester-differentiated myeloid cells contain PDGF-BR molecules of 37 kDa to 130 kDa. The results also show that the tandemly linked genes for PDGF-BR and the macrophage colony-stimulating factor 1 receptor are coexpressed in the phorbol ester-differentiated myeloid cells. Expression of these two receptor genes has not been shown previously in any cell type to our knowledge.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1c0d0446115293cb8ab2a7b93730d375" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818090,"asset_id":21328265,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818090/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328265"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328265"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328265; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328265]").text(description); $(".js-view-count[data-work-id=21328265]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328265; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328265']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1c0d0446115293cb8ab2a7b93730d375" } } $('.js-work-strip[data-work-id=21328265]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328265,"title":"Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation","internal_url":"https://www.academia.edu/21328265/Coexpression_of_the_genes_for_platelet_derived_growth_factor_B_chain_receptor_and_macrophage_colony_stimulating_factor_1_receptor_during_monocytic_differentiation","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818090,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818090/thumbnails/1.jpg","file_name":"Coexpression_of_the_genes_for_platelet-d20160131-9418-nkpkk4.pdf","download_url":"https://www.academia.edu/attachments/41818090/download_file","bulk_download_file_name":"Coexpression_of_the_genes_for_platelet_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818090/Coexpression_of_the_genes_for_platelet-d20160131-9418-nkpkk4-libre.pdf?1454277111=\u0026response-content-disposition=attachment%3B+filename%3DCoexpression_of_the_genes_for_platelet_d.pdf\u0026Expires=1740946885\u0026Signature=EfACDfnnMjFBk6I4xAa~kmSFMkq-F8OTs6-GzYS~Kcw-N4gVvzS50VQarNGGkg-EFXNmHaF4N6OX~81i6h70oyd8SHTKpfpJqGSOEV~7mrQ8jABQWeGSckL~nOlW78i1lVFSvfeP3XgopDH5pCiPUBg43~D0mTVGTS3x-riQoKGBUK9NtD~YhzYUb9sjUsdce5IkFGO0kuvPB4qR7x5-y7Cm1mIYlVnz6yW0qxT8-7HwStzspLmle47k2omZevnIvwBjnTVK98UwuWaZnDBGoSce544x6jU1DyBK2zNgIjHsxeB630n2zm3B157S4AEE446-CQjKUH817bjP0bNoxw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328264"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328264/Cell_type_specific_pattern_of_myc_protooncogene_expression_in_developing_human_embryos"><img alt="Research paper thumbnail of Cell-type-specific pattern of myc protooncogene expression in developing human embryos" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328264/Cell_type_specific_pattern_of_myc_protooncogene_expression_in_developing_human_embryos">Cell-type-specific pattern of myc protooncogene expression in developing human embryos</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1985</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The expression of viral oncogenes in cells transformed by acutely transforming retroviruses profo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The expression of viral oncogenes in cells transformed by acutely transforming retroviruses profoundly alters proliferation and differentiation in the target cell, suggesting that the cellular homologues of the viral oncogenes, the protooncogenes, have a role in normal cell proliferation and differentiation. To investigate the possible developmental role of protooncogenes in human embryogenesis, we have determined the spatial distribution of myc gene transcripts in early human embryos by using in situ hybridization of a labeled myc exon to thin sections. The results indicate a stage- and cell-type-specific regulation of c-myc gene expression in primarily epithelial cells of late first trimester embryos. Furthermore, the data suggest that the linkage between c-myc gene expression and cellular proliferation holds for only a restricted set of embryonic cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328264"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328264"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328264; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328264]").text(description); $(".js-view-count[data-work-id=21328264]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328264; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328264']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328264]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328264,"title":"Cell-type-specific pattern of myc protooncogene expression in developing human embryos","internal_url":"https://www.academia.edu/21328264/Cell_type_specific_pattern_of_myc_protooncogene_expression_in_developing_human_embryos","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328263"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328263/Induction_of_c_sis_mRNA_and_activity_similar_to_platelet_derived_growth_factor_by_transforming_growth_factor_beta_a_proposed_model_for_indirect_mitogenesis_involving_autocrine_activity"><img alt="Research paper thumbnail of Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328263/Induction_of_c_sis_mRNA_and_activity_similar_to_platelet_derived_growth_factor_by_transforming_growth_factor_beta_a_proposed_model_for_indirect_mitogenesis_involving_autocrine_activity">Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1986</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth fac...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor beta resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium. In addition, PDGF-regulated genes (c-fos and c-myc) were stimulated by transforming growth factor beta with delayed kinetics relative to that seen in other cell systems with direct PDGF stimulation. A model is proposed in which the monolayer mitogenicity of transforming growth factor beta is mediated by the induction of c-sis and PDGF and the subsequent autocrine stimulation of c-fos, c-myc, and other PDGF-inducible genes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328263"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328263"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328263; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328263]").text(description); $(".js-view-count[data-work-id=21328263]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328263; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328263']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328263]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328263,"title":"Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity","internal_url":"https://www.academia.edu/21328263/Induction_of_c_sis_mRNA_and_activity_similar_to_platelet_derived_growth_factor_by_transforming_growth_factor_beta_a_proposed_model_for_indirect_mitogenesis_involving_autocrine_activity","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328262"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328262/Integration_of_proviral_DNA_into_the_PDGF_%CE%B2_receptor_gene_in_HTLV_I_infected_T_cells_results_in_a_novel_tyrosine_kinase_product_with_transforming_activity"><img alt="Research paper thumbnail of Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity" class="work-thumbnail" src="https://attachments.academia-assets.com/41818092/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328262/Integration_of_proviral_DNA_into_the_PDGF_%CE%B2_receptor_gene_in_HTLV_I_infected_T_cells_results_in_a_novel_tyrosine_kinase_product_with_transforming_activity">Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity</a></div><div class="wp-workCard_item"><span>Oncogene</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have previously shown that noninfected human T-cell lines express the canonical 5.7 kb mRNA co...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have previously shown that noninfected human T-cell lines express the canonical 5.7 kb mRNA coding for the type b platelet-derived growth factor-receptor (PDGF breceptor), whereas HTLV-I-infected T-cell lines express a novel PDGF b-receptor mRNA of 3.8 kb. In this report, we have extended those studies to molecularly characterize the 3.8 kb PDGF b-receptor mRNA and show that it has resulted from integration of an apparently undeleted HTLV-I provirus into the PDGF b-receptor gene in an orientation enabling expression of a truncated PDGF b-receptor mRNA using the 3' HTLV-I long terminal repeat as a promoter. Further, NIH3T3 cells transfected with a plasmid containing the truncated PDGF b-receptor ORF plasmid generate colonies in soft agar with more cells per colony than untransfected cells, or cells transfected with the Tax 1 or PDGF-B (c-sis) plasmids. These results indicate that the truncated PDGF b-receptor protein acquires transforming capability and that HTLV-I-induced truncation of PDGF b-receptor may correlate with HTLV-I-associated neoplasia of human T-cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="684e30daad039b51a0be95b32cd2da85" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818092,"asset_id":21328262,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818092/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328262"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328262"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328262; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328262]").text(description); $(".js-view-count[data-work-id=21328262]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328262; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328262']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "684e30daad039b51a0be95b32cd2da85" } } $('.js-work-strip[data-work-id=21328262]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328262,"title":"Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity","internal_url":"https://www.academia.edu/21328262/Integration_of_proviral_DNA_into_the_PDGF_%CE%B2_receptor_gene_in_HTLV_I_infected_T_cells_results_in_a_novel_tyrosine_kinase_product_with_transforming_activity","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818092,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818092/thumbnails/1.jpg","file_name":"Integration_of_proviral_DNA_into_the_PDG20160131-1245-bfykth.pdf","download_url":"https://www.academia.edu/attachments/41818092/download_file","bulk_download_file_name":"Integration_of_proviral_DNA_into_the_PDG.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818092/Integration_of_proviral_DNA_into_the_PDG20160131-1245-bfykth-libre.pdf?1454277112=\u0026response-content-disposition=attachment%3B+filename%3DIntegration_of_proviral_DNA_into_the_PDG.pdf\u0026Expires=1740946885\u0026Signature=B0Q-e4xs1lb8sheNlS49uoT~MEn2Nni7JsISS77P08iAyKa2xrPFWORdN4oRtm4~qLEARTmf4cc-uD83DQP-PqSToWoX92VEG3gvJAfSLpMAcZKtNfQJPeDJQ-7eDaHDiWemqhcluSb2jAf7zgRB6xkbfstZgAknPWlCRkir~~Za~FAkStwYq3aR9mmLAimJSdvz0MjigUlkJOZpnz1A26ArxnXd9EmpaC~d-CGi8VcQm3aaGWikhwKOoLj3DrJjmsiQsTz0xEtUMaSxsawXcz-mFwkfp8EQTB-6C-fnHwqmwgKyzNeJgB9J6bd3H~R9vuvUUQ7uEm83tEIJWD-1BQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328261"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328261/Absolute_quantification_of_target_DNA_a_simple_competitive_PCR_for_efficient_analysis_of_multiple_samples"><img alt="Research paper thumbnail of Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples" class="work-thumbnail" src="https://attachments.academia-assets.com/41818119/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328261/Absolute_quantification_of_target_DNA_a_simple_competitive_PCR_for_efficient_analysis_of_multiple_samples">Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://wayne.academia.edu/AGoustin">A. Goustin</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/VladimirZachar">Vladimir Zachar</a></span></div><div class="wp-workCard_item"><span>Nucleic Acids Research</span><span>, 1993</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponen...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponential nature of DNA amplification, however, is prone to burden the experimental data with significant standard error due to the tube-to-tube inherent variations in amplification efficiency. Therefore the reliable quantitation of DNA or RNA templates by PCR necessitates introduction of an internal standard. Typically, in a series of replicates of analyzed sample the defined range of such standards is coamplified together with constant unknown amount of wildtype template (1, 2) or alternatively both templates are subjected to endpoint dilution (3). Obviously, each of the methods is based on multiple PCR reactions which are to be performed simultaneously with each of the analyzed samples. Such procedure, nevertheless, may become cumbersome when large numbers of samples are to be analyzed. A INPUT 429 bp TEMPLATE [pg] be BE 4O 429 bp 4 222 bp</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a88c044d444fa94c49fe186d1bf96065" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818119,"asset_id":21328261,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818119/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328261"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328261"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328261; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328261]").text(description); $(".js-view-count[data-work-id=21328261]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328261; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328261']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a88c044d444fa94c49fe186d1bf96065" } } $('.js-work-strip[data-work-id=21328261]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328261,"title":"Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples","internal_url":"https://www.academia.edu/21328261/Absolute_quantification_of_target_DNA_a_simple_competitive_PCR_for_efficient_analysis_of_multiple_samples","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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Goustin</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RobertCoffey4">Robert Coffey</a></span></div><div class="wp-workCard_item"><span>Nature</span><span>, 1987</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dca38447e66cbfdf9e6b0bd87b04eb31" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818088,"asset_id":21328260,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818088/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328260"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328260"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328260; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328260]").text(description); $(".js-view-count[data-work-id=21328260]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328260; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328260']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dca38447e66cbfdf9e6b0bd87b04eb31" } } $('.js-work-strip[data-work-id=21328260]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328260,"title":"Production and auto-induction of transforming growth factor-α in human keratinocytes","internal_url":"https://www.academia.edu/21328260/Production_and_auto_induction_of_transforming_growth_factor_%CE%B1_in_human_keratinocytes","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818088,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818088/thumbnails/1.jpg","file_name":"Production_and_auto-Induction_of_transfo20160131-6055-1lw9oam.pdf","download_url":"https://www.academia.edu/attachments/41818088/download_file","bulk_download_file_name":"Production_and_auto_induction_of_transfo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818088/Production_and_auto-Induction_of_transfo20160131-6055-1lw9oam-libre.pdf?1454277111=\u0026response-content-disposition=attachment%3B+filename%3DProduction_and_auto_induction_of_transfo.pdf\u0026Expires=1740946885\u0026Signature=L5jBlaaL1mxb9DEEQqCUOZerb~RKav9wGgJs90KKDB~nw4ROhgzTr1gtWp3LwCvhIVPrQTQzNgGGLBMRXlcCuf1sd~uLy0MGVgZwiojuFbV8I3G6xayrALQRG-UfUVUFR-IQf4vEe74s4j1e0xHLcPArKgcNZJzy7SlRYS2Gs1cE3M56FE8voAEjrHltBw2PKd2-daPK7nPupokqh4y1J3Y-LTEfVFEsjJTInDUC5LXZk3F1UAW9inGjN0qXY7HGQfyT2NmLHo5YX6UGH7szxWqMOzrcd4QO9m2enwdgQgCNFTN2Isr~ZjMGwuabyj5W8UGUSFG7ntVZpgXZilKZEw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328259"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328259/Serum_alpha_2_HS_glycoprotein_is_an_inhibitor_of_the_human_insulin_receptor_at_the_tyrosine_kinase_level"><img alt="Research paper thumbnail of Serum alpha 2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level" class="work-thumbnail" src="https://attachments.academia-assets.com/41904399/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328259/Serum_alpha_2_HS_glycoprotein_is_an_inhibitor_of_the_human_insulin_receptor_at_the_tyrosine_kinase_level">Serum alpha 2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level</a></div><div class="wp-workCard_item"><span>Molecular Endocrinology</span><span>, 1993</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The insulin-dependent tyrosine kinase activity (TKA) of the insulin receptor (IR) plays an essent...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The insulin-dependent tyrosine kinase activity (TKA) of the insulin receptor (IR) plays an essential role in insulin signaling. Thus, dysregulation of IR-TKA might be an important element in the states of insulin resistance. A phosphorylated rat hepatic glycoprotein (pp63) acting as an inhibitor of IR-TK has been described. In search of the human homolog of pp63, we isolated a cDNA clone from a human liver lambda gt11 cDNA library. DNA sequence analysis reveals identity with the mRNA product of a human gene AHSG encoding a serum protein, alpha 2-Heremans Scmid-glycoprotein (alpha 2HSG), with heretofore unknown physiological function. Northern blot analysis demonstrates a 1.8-kilobase mRNA in human liver and HepG2 hepatoma cells. alpha 2HSG, purified from human serum, specifically inhibits insulin-stimulated IR autophosphorylation in vitro and in vivo as well as exogenous substrate tyrosine phosphorylation. alpha 2HSG also inhibits both insulin-induced tyrosine phosphorylation of IRS-1 and the association of IRS-1 with the p85 subunit of phosphatidylinositol-3 kinase in H-35 hepatoma cells. alpha 2HSG inhibits insulin-dependent mitogenesis, but does not affect insulin-stimulated induction of the metabolic enzyme tyrosine aminotransferase. alpha 2HSG does not compete with insulin for binding to IR. Finally, the action of alpha 2HSG is specific toward the IR-TK; its effect does not extend to insulin-like growth factor-I-stimulated TKA. Our results allow us to assign a biochemical function for human alpha 2HSG, namely regulation of insulin action at the IR-TK level.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a47dc553fac5ddfe6f5c662b04dc844d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41904399,"asset_id":21328259,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41904399/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328259"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328259"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328259; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328259]").text(description); $(".js-view-count[data-work-id=21328259]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328259; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328259']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a47dc553fac5ddfe6f5c662b04dc844d" } } $('.js-work-strip[data-work-id=21328259]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328259,"title":"Serum alpha 2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level","internal_url":"https://www.academia.edu/21328259/Serum_alpha_2_HS_glycoprotein_is_an_inhibitor_of_the_human_insulin_receptor_at_the_tyrosine_kinase_level","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41904399,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41904399/thumbnails/1.jpg","file_name":"Serum_alpha_2-HS_glycoprotein_is_an_inhi20160202-26443-174n6iq.pdf","download_url":"https://www.academia.edu/attachments/41904399/download_file","bulk_download_file_name":"Serum_alpha_2_HS_glycoprotein_is_an_inhi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41904399/Serum_alpha_2-HS_glycoprotein_is_an_inhi20160202-26443-174n6iq-libre.pdf?1454454255=\u0026response-content-disposition=attachment%3B+filename%3DSerum_alpha_2_HS_glycoprotein_is_an_inhi.pdf\u0026Expires=1740946885\u0026Signature=er0ViQgGDXwAxvBIKHga6V6my5JO77GjpuLxdsePCIe7yznfRL9qJNiHA8Ve-UDyYiZeiFTyn64wG-0mp5WG2~ANeTyuZmcHqK33~QxXJ-P1~suXxdHcmiIKNiLvU36iC9PV23DF1vTQBi4IFaNmKaiF8M7~kRGhsjsjFF-GVTJWGX96MO~7iRkucBv3Z30gSq00QLMTG87xzNsV880SEapsqSqRVe7ZNWScqR~B0mxr6ZZTq9PUj2shMcYb0URJwN~7TtDhV78Tt1JL2abFXfA9brgMsM1O370PNSavvzjWn96l7XqYuS1uCgLIbrlyj8QSz5kzA4XXlBRZZ5VO2g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328258"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328258/Role_for_Human_SIRT2_NAD_Dependent_Deacetylase_Activity_in_Control_of_Mitotic_Exit_in_the_Cell_Cycle"><img alt="Research paper thumbnail of Role for Human SIRT2 NAD-Dependent Deacetylase Activity in Control of Mitotic Exit in the Cell Cycle" class="work-thumbnail" src="https://attachments.academia-assets.com/41818083/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328258/Role_for_Human_SIRT2_NAD_Dependent_Deacetylase_Activity_in_Control_of_Mitotic_Exit_in_the_Cell_Cycle">Role for Human SIRT2 NAD-Dependent Deacetylase Activity in Control of Mitotic Exit in the Cell Cycle</a></div><div class="wp-workCard_item"><span>Molecular and Cellular Biology</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Studies of yeast have shown that the SIR2 gene family is involved in chromatin structure, transcr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Studies of yeast have shown that the SIR2 gene family is involved in chromatin structure, transcriptional silencing, DNA repair, and control of cellular life span. Our functional studies of human SIRT2, a homolog of the product of the yeast SIR2 gene, indicate that it plays a role in mitosis. The SIRT2 protein is a NADdependent deacetylase (NDAC), the abundance of which increases dramatically during mitosis and is multiply phosphorylated at the G 2 /M transition of the cell cycle. Cells stably overexpressing the wild-type SIRT2 but not missense mutants lacking NDAC activity show a marked prolongation of the mitotic phase of the cell cycle. Overexpression of the protein phosphatase CDC14B, but not its close homolog CDC14A, results in dephosphorylation of SIRT2 with a subsequent decrease in the abundance of SIRT2 protein. A CDC14B mutant defective in catalyzing dephosphorylation fails to change the phosphorylation status or abundance of SIRT2 protein. Addition of 26S proteasome inhibitors to human cells increases the abundance of SIRT2 protein, indicating that SIRT2 is targeted for degradation by the 26S proteasome. Our data suggest that human SIRT2 is part of a phosphorylation cascade in which SIRT2 is phosphorylated late in G 2 , during M, and into the period of cytokinesis. CDC14B may provoke exit from mitosis coincident with the loss of SIRT2 via ubiquitination and subsequent degradation by the 26S proteasome.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="076a6395f3379a5f7591bf750e302ccb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818083,"asset_id":21328258,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818083/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328258"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328258"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328258; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328258]").text(description); $(".js-view-count[data-work-id=21328258]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328258; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328258']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "076a6395f3379a5f7591bf750e302ccb" } } $('.js-work-strip[data-work-id=21328258]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328258,"title":"Role for Human SIRT2 NAD-Dependent Deacetylase Activity in Control of Mitotic Exit in the Cell Cycle","internal_url":"https://www.academia.edu/21328258/Role_for_Human_SIRT2_NAD_Dependent_Deacetylase_Activity_in_Control_of_Mitotic_Exit_in_the_Cell_Cycle","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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Goustin</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://wayne.academia.edu/ChristopherSinkler">Christopher Sinkler</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LeeSennottmiller">Lee Sennott-miller</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JLiu13">J. Liu</a></span></div><div class="wp-workCard_item"><span>Cytochromes b and c: Biochemical Properties, Biological Functions and Electrochemical Analysis </span><span>, Mar 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Despite the fact that over 200 phosphorylation sites have been mapped on the mitochondrial oxidat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Despite the fact that over 200 phosphorylation sites have been mapped on the mitochondrial oxidative phosphorylation (OxPhos) complexes, very little is known about the relevant cell signaling pathways and the terminal kinases and phosphatases that control these phosphorylations. Within OxPhos, cytochrome c (Cytc) plays a special role because it is not only involved in electron transport but is also a key executer of apoptosis when it is released from the mitochondria. It is therefore not surprising that Cytc is regulated by phosphorylation. Four phosphorylation sites have been mapped on mammalian Cytc, two of which have been studied functionally, demonstrating that both respiration and apoptosis are under the control of signaling pathways that have yet to be identified. We here review the regulation and multiple functions of mammalian Cytc, including respiration, reactive oxygen species (ROS) scavenging under healthy conditions, ROS production via p66Shc, and cardiolipin oxidation during apoptosis. We propose targeting Cytc by manipulation of signaling cascades as a therapeutic avenue in conditions including neurodegeneration and cancer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f49a4e63648d89b94b1378082e4fe40d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":33258566,"asset_id":6471532,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/33258566/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="6471532"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="6471532"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6471532; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="3424288" id="papers"><div class="js-work-strip profile--work_container" data-work-id="47674462"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/47674462/Protein_synthesis_polyribosomes_and_peptide_elongation_in_early_development_of_Strongylocentrotus_purpuratus"><img alt="Research paper thumbnail of Protein synthesis, polyribosomes, and peptide elongation in early development of Strongylocentrotus purpuratus" class="work-thumbnail" src="https://attachments.academia-assets.com/66644681/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/47674462/Protein_synthesis_polyribosomes_and_peptide_elongation_in_early_development_of_Strongylocentrotus_purpuratus">Protein synthesis, polyribosomes, and peptide elongation in early development of Strongylocentrotus purpuratus</a></div><div class="wp-workCard_item"><span>Developmental Biology</span><span>, 1981</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The absolute rate of protein synthesis in developing embryos of Strm&wentrotvs purpwatw has been ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The absolute rate of protein synthesis in developing embryos of Strm&wentrotvs purpwatw has been measured by lysine incorporation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ad17207400022136d4095c3d725b8b39" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":66644681,"asset_id":47674462,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/66644681/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="47674462"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="47674462"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 47674462; 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Membrane-associated teichuronic acid was extracted from the wall-membrane fraction of reaction mixtures by sodium dodecyl sulfate. The synthesis of membrane-associated teichuronic acid required UDP-glucose, UDP-ManNAcUA, and UDP-N-acetylglucosamine and was inhibited by tunicamycin. Glucose incorporated into wall-bound teichuronic acid remained in wall fragments after extraction with sodium dodecyl sulfate, and its incorporation required UDP-glucose and UDP-ManNAcUA (but not UDP-N-acetylglucosamine) and was insensitive to tunicamycin. Radioactive material incorporated into wall-bound teichuronic acid could be released by treatment with mild acid or by digestion with lysozyme, indicating that the...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328277"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328277"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328277; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328277]").text(description); $(".js-view-count[data-work-id=21328277]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328277; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328277']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328277]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328277,"title":"Elongation of teichuronic acid chains by a wall-membrane preparation from Micrococcus luteus","internal_url":"https://www.academia.edu/21328277/Elongation_of_teichuronic_acid_chains_by_a_wall_membrane_preparation_from_Micrococcus_luteus","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328276"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328276/Direct_measurement_of_histone_peptide_elongation_rate_in_cleaving_sea_urchin_embryos"><img alt="Research paper thumbnail of Direct measurement of histone peptide elongation rate in cleaving sea urchin embryos" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328276/Direct_measurement_of_histone_peptide_elongation_rate_in_cleaving_sea_urchin_embryos">Direct measurement of histone peptide elongation rate in cleaving sea urchin embryos</a></div><div class="wp-workCard_item"><span>Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression</span><span>, 1982</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Regulation of protein synthesis can be exercised at a number of levels. One of the more experimen...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Regulation of protein synthesis can be exercised at a number of levels. One of the more experimentally difficult levels to approach has been the measurement of peptide elongation rate. This paper presents a new application of the cyanogen bromide (CNBr) cleavage of proteins in a direct measurement of histone peptide elongation rate in cleaving sea urchin embryos (Strongylocentrotus purpuratus). The data indicate an elongation rate (at 15 degrees C) for histones H2B and H1 alpha of 0.69 and 0.80 codons per s, respectively. These values fall within the range of previously published values of average peptide elongation rate for total protein in these cells. This method should be generally applicable to many systems for which the measurement of peptide elongation rate may provide a key to the understanding of the regulation of protein synthesis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328276"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328276"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328276; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328276]").text(description); $(".js-view-count[data-work-id=21328276]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328276; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328276']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328276]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328276,"title":"Direct measurement of histone peptide elongation rate in cleaving sea urchin embryos","internal_url":"https://www.academia.edu/21328276/Direct_measurement_of_histone_peptide_elongation_rate_in_cleaving_sea_urchin_embryos","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328272"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328272/Genetic_polymorphism_of_envelope_V3_region_of_HIV_type_1_subtypes_A_C_and_D_from_Nairobi_Kenya"><img alt="Research paper thumbnail of Genetic polymorphism of envelope V3 region of HIV type 1 subtypes A, C, and D from Nairobi, Kenya" class="work-thumbnail" src="https://attachments.academia-assets.com/41818091/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328272/Genetic_polymorphism_of_envelope_V3_region_of_HIV_type_1_subtypes_A_C_and_D_from_Nairobi_Kenya">Genetic polymorphism of envelope V3 region of HIV type 1 subtypes A, C, and D from Nairobi, Kenya</a></div><div class="wp-workCard_item"><span>AIDS research and human retroviruses</span><span>, 1996</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7adfbe807962dda9e726c571d1f0f1c5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818091,"asset_id":21328272,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818091/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328272"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328272"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328272; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328272]").text(description); $(".js-view-count[data-work-id=21328272]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328272; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328272']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7adfbe807962dda9e726c571d1f0f1c5" } } $('.js-work-strip[data-work-id=21328272]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328272,"title":"Genetic polymorphism of envelope V3 region of HIV type 1 subtypes A, C, and D from Nairobi, Kenya","internal_url":"https://www.academia.edu/21328272/Genetic_polymorphism_of_envelope_V3_region_of_HIV_type_1_subtypes_A_C_and_D_from_Nairobi_Kenya","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328271"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328271/Spread_of_HIV_type_1_in_Slovakia_remains_limited_and_is_restricted_to_subtype_B"><img alt="Research paper thumbnail of Spread of HIV type 1 in Slovakia remains limited and is restricted to subtype B" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328271/Spread_of_HIV_type_1_in_Slovakia_remains_limited_and_is_restricted_to_subtype_B">Spread of HIV type 1 in Slovakia remains limited and is restricted to subtype B</a></div><div class="wp-workCard_item"><span>AIDS research and human retroviruses</span><span>, Jan 20, 1996</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328271"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328271"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328271; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328271]").text(description); $(".js-view-count[data-work-id=21328271]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328271; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328271']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328271]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328271,"title":"Spread of HIV type 1 in Slovakia remains limited and is restricted to subtype B","internal_url":"https://www.academia.edu/21328271/Spread_of_HIV_type_1_in_Slovakia_remains_limited_and_is_restricted_to_subtype_B","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328270"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328270/Recombinant_human_alpha_2_HS_glycoprotein_inhibits_insulin_stimulated_mitogenic_pathway_without_affecting_metabolic_signalling_in_Chinese_hamster_ovary_cells_overexpressing_the_human_insulin_receptor"><img alt="Research paper thumbnail of Recombinant human alpha 2-HS glycoprotein inhibits insulin-stimulated mitogenic pathway without affecting metabolic signalling in Chinese hamster ovary cells overexpressing the human insulin receptor" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328270/Recombinant_human_alpha_2_HS_glycoprotein_inhibits_insulin_stimulated_mitogenic_pathway_without_affecting_metabolic_signalling_in_Chinese_hamster_ovary_cells_overexpressing_the_human_insulin_receptor">Recombinant human alpha 2-HS glycoprotein inhibits insulin-stimulated mitogenic pathway without affecting metabolic signalling in Chinese hamster ovary cells overexpressing the human insulin receptor</a></div><div class="wp-workCard_item"><span>Cellular signalling</span><span>, 1996</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Insulin acts on its target tissues by specific interaction with the cell surface insulin receptor...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Insulin acts on its target tissues by specific interaction with the cell surface insulin receptor (IR). The IR possesses an intrinsic tyrosine kinase (TK) activity which is stimulated by insulin binding. This TK activity is required for many aspects of insulin signalling. We had earlier reported that human plasma alpha 2-HS glycoprotein (alpha 2-HSG) inhibits insulin-stimulated mitogenesis at the level of IR-TK (Mol Endo 7: 1445-1455, 1993). In the present study, using recombinant alpha 2-HSG, which possesses 50-100 times the specific activity of plasma alpha 2-HSG, we have further investigated the molecular basis of this effect. We examined the insulin-stimulated Ras signalling pathway in Chinese Hamster Ovary cells overexpressing the human IR. alpha 2-HSG inhibits insulin-induced tyrosine phosphorylation of IRS-1 and the subsequent association of GRB2, as well as Sos, with IRS-1. This inhibition results in reduced guanine nucleotide exchange in p21ras. alpha 2-HSG also inhibits th...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328270"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328270"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328270; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328270]").text(description); $(".js-view-count[data-work-id=21328270]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328270; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328270']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328270]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328270,"title":"Recombinant human alpha 2-HS glycoprotein inhibits insulin-stimulated mitogenic pathway without affecting metabolic signalling in Chinese hamster ovary cells overexpressing the human insulin receptor","internal_url":"https://www.academia.edu/21328270/Recombinant_human_alpha_2_HS_glycoprotein_inhibits_insulin_stimulated_mitogenic_pathway_without_affecting_metabolic_signalling_in_Chinese_hamster_ovary_cells_overexpressing_the_human_insulin_receptor","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328269"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328269/Alpha2_HSG_a_specific_inhibitor_of_insulin_receptor_autophosphorylation_interacts_with_the_insulin_receptor"><img alt="Research paper thumbnail of Alpha2-HSG, a specific inhibitor of insulin receptor autophosphorylation, interacts with the insulin receptor" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328269/Alpha2_HSG_a_specific_inhibitor_of_insulin_receptor_autophosphorylation_interacts_with_the_insulin_receptor">Alpha2-HSG, a specific inhibitor of insulin receptor autophosphorylation, interacts with the insulin receptor</a></div><div class="wp-workCard_item"><span>Molecular and cellular endocrinology</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human fetuin, [alpha2-Heremans Schmid Glycoprotein (alpha2-HSG)], is a natural inhibitor of insul...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human fetuin, [alpha2-Heremans Schmid Glycoprotein (alpha2-HSG)], is a natural inhibitor of insulin receptor tyrosine kinase activity (IR-TKA). Previously, we have demonstrated that alpha2-HSG inhibits the mitogenic pathway without affecting the metabolic arm of insulin signal transduction. In this study, we demonstrate the time-course and specificity of inhibition, its interaction with IR and probable physiological role. In intact rat1 fibroblasts overexpressing the human insulin receptor (HIRc B), incubation of recombinant human alpha2-HSGbac (1.8 microM) inhibited insulin-induced IR autophosphorylation by over 80%. This inhibitory effect of alpha2-HSGbac on insulin-induced IR autophosphorylation was blunted by half in 60 min. Interestingly, alpha2-HSGbac at similar concentrations (0.9 or 1.8 microM), had no effect on EGF- or IGF-I-induced cognate receptor autophosphorylation. Anti-alpha2-HSG immunoprecipitates of alpha2-HSGbac-treated HIRc B cell lysates demonstrated the presence...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328269"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328269"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328269; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328269]").text(description); $(".js-view-count[data-work-id=21328269]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328269; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328269']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328269]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328269,"title":"Alpha2-HSG, a specific inhibitor of insulin receptor autophosphorylation, interacts with the insulin receptor","internal_url":"https://www.academia.edu/21328269/Alpha2_HSG_a_specific_inhibitor_of_insulin_receptor_autophosphorylation_interacts_with_the_insulin_receptor","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328268"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328268/Genetic_Mapping_and_Functional_Studies_of_a_Natural_Inhibitor_of_the_Insulin_Receptor_Tyrosine_Kinase_The_Mouse_Ortholog_of_Human_%CE%B12_HS_Glycoprotein"><img alt="Research paper thumbnail of Genetic Mapping and Functional Studies of a Natural Inhibitor of the Insulin Receptor Tyrosine Kinase: The Mouse Ortholog of Human α2-HS Glycoprotein" class="work-thumbnail" src="https://attachments.academia-assets.com/41818095/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328268/Genetic_Mapping_and_Functional_Studies_of_a_Natural_Inhibitor_of_the_Insulin_Receptor_Tyrosine_Kinase_The_Mouse_Ortholog_of_Human_%CE%B12_HS_Glycoprotein">Genetic Mapping and Functional Studies of a Natural Inhibitor of the Insulin Receptor Tyrosine Kinase: The Mouse Ortholog of Human α2-HS Glycoprotein</a></div><div class="wp-workCard_item"><span>International Journal of Experimental Diabetes Research</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">IR-TKA in vitro. In addition, mouse o2-HSG (25tg/ml) completely abolishes insulin-induced DNA syn...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">IR-TKA in vitro. In addition, mouse o2-HSG (25tg/ml) completely abolishes insulin-induced DNA synthesis in H-35 rat hepatoma cells. Based on the sequence data and functional analysis, we conclude that the mouse Ahsg gene is the true ortholog of the human AHSG gene. 249 250 V.J. CINTRON et al. reactant protein and altered concentrations of c2-HSG have been reported in several disease conditions including Paget's disease, osteogenesis imperfecta, lymphoma, leukemia and myelofibrosis. 5, 61 Several functions have been attributed to c2-HSG, including its involvement in immune response, the chemotactic response of macrophages, 8, 91 enhancement of phagocytic function of human monocytes, bone mineralization, bone accumulation, calcification and as an inhibitor of insulin receptor tyrosine kinase activity [IR-TKA;18]. Recently, Jahnen-Dechent et al. demonstrated that mice deficient for c2-HSG can develop ectopic microcalcifications in soft tissues, supporting the idea that c2-HSG may operate as an inhibitor of apatite crystal growth in vivo.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="713931f805e3c4e8c33b288424ad79e4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818095,"asset_id":21328268,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818095/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328268"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328268"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328268; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328268]").text(description); $(".js-view-count[data-work-id=21328268]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328268; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328268']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "713931f805e3c4e8c33b288424ad79e4" } } $('.js-work-strip[data-work-id=21328268]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328268,"title":"Genetic Mapping and Functional Studies of a Natural Inhibitor of the Insulin Receptor Tyrosine Kinase: The Mouse Ortholog of Human α2-HS Glycoprotein","internal_url":"https://www.academia.edu/21328268/Genetic_Mapping_and_Functional_Studies_of_a_Natural_Inhibitor_of_the_Insulin_Receptor_Tyrosine_Kinase_The_Mouse_Ortholog_of_Human_%CE%B12_HS_Glycoprotein","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818095,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818095/thumbnails/1.jpg","file_name":"Genetic_Mapping_and_Functional_Studies_o20160131-9433-96wh78.pdf","download_url":"https://www.academia.edu/attachments/41818095/download_file","bulk_download_file_name":"Genetic_Mapping_and_Functional_Studies_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818095/Genetic_Mapping_and_Functional_Studies_o20160131-9433-96wh78-libre.pdf?1454277111=\u0026response-content-disposition=attachment%3B+filename%3DGenetic_Mapping_and_Functional_Studies_o.pdf\u0026Expires=1740946885\u0026Signature=BAVrAMaKojh0r5DDjXSX39QtFlOJfms5i-SDczkJUUDIeYxh3YYE~P9b8PL1v0FSRUn3-U7myaYMAnWWoM3csPtRLcjzJBtdZS-ElEklfA-7G-tRg~ZCxc5tj9hnwiQVUfWokhfZ4JT80C336mHelTwR3sf~0Z8zkmuCcz9a~xtTY8uCKEIMw9RtFxRx3GjU2QBzr0m2Vw33vqJFWB4DneOAaLPI0rYHZJkp~5uLeWCeFywEYMvW6h7pv~guuV6suq7zBBV1iOwCjeOfWw~H7pDYKfT7B6FpIn664Q37CMWQksarJQlIF04Rj5EwTlEbvv~Te~I9YcDlGTJ2LgS~vw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328267"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328267/Spatial_and_temporal_pattern_of_cellular_myc_oncogene_expression_in_developing_human_placenta_Implications_for_embryonic_cell_proliferation"><img alt="Research paper thumbnail of Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328267/Spatial_and_temporal_pattern_of_cellular_myc_oncogene_expression_in_developing_human_placenta_Implications_for_embryonic_cell_proliferation">Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation</a></div><div class="wp-workCard_item"><span>Cell</span><span>, 1984</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have analyzed staged human placentas by Northern, dot blot, and in situ hybridization to human...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have analyzed staged human placentas by Northern, dot blot, and in situ hybridization to human c-myc probes. Placental RNA exhibits a stage-specific appearance of a 2.4 kb transcript of the c-myc gene. The frequency of this transcript varies 20 to 30 fold over the course of placental development, showing a peak at 4-5 weeks after conception, where the myc transcripts comprise about 0.05% by weight of the total placental mRNA. A clear decline in placental c-myc transcription is seen before the end of the first trimester of pregnancy. In situ hybridization to 125I-labeled myc probes demonstrates an unequal spatial distribution of myc transcripts in placental with particularly high expression in the cytotrophoblastic shell of early placenta. Labeling of placental explants with 3H-thymidine, the localization of myc transcripts to cytotrophoblasts, and the temporal pattern of myc expression all support a strong correlation between myc transcript abundance and cytotrophoblast proliferation. We argue for a role for the c-myc gene in the proliferation of normal cells in this tissue.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328267"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328267"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328267; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328267]").text(description); $(".js-view-count[data-work-id=21328267]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328267; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328267']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328267]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328267,"title":"Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation","internal_url":"https://www.academia.edu/21328267/Spatial_and_temporal_pattern_of_cellular_myc_oncogene_expression_in_developing_human_placenta_Implications_for_embryonic_cell_proliferation","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328266"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328266/Sequence_Note_Genetic_Polymorphism_of_Envelope_V3_Region_of_HIV_Type_1_Subtypes_A_C_and_D_from_Nairobi_Kenya"><img alt="Research paper thumbnail of Sequence Note : Genetic Polymorphism of Envelope V3 Region of HIV Type 1 Subtypes A, C, and D from Nairobi, Kenya" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328266/Sequence_Note_Genetic_Polymorphism_of_Envelope_V3_Region_of_HIV_Type_1_Subtypes_A_C_and_D_from_Nairobi_Kenya">Sequence Note : Genetic Polymorphism of Envelope V3 Region of HIV Type 1 Subtypes A, C, and D from Nairobi, Kenya</a></div><div class="wp-workCard_item"><span>Aids Research and Human Retroviruses</span><span>, 1996</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">... J Virol 1995;69:263-271. 5. Ebbesen P, Hager H, Norskov-Lauritsen N, Aboagye-Mathiesen G, Zdr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... J Virol 1995;69:263-271. 5. Ebbesen P, Hager H, Norskov-Lauritsen N, Aboagye-Mathiesen G, Zdravkovic M, Villadsen J, Liu X, Petersen PM, Bambra C, Nyongo Page 4. 78 ZACHAR ET AL. A, Temmerman M, and Zachar V: Concurrence of high levels of in-</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328266"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328266"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328266; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328266]").text(description); $(".js-view-count[data-work-id=21328266]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328266; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328266']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328266]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328266,"title":"Sequence Note : Genetic Polymorphism of Envelope V3 Region of HIV Type 1 Subtypes A, C, and D from Nairobi, Kenya","internal_url":"https://www.academia.edu/21328266/Sequence_Note_Genetic_Polymorphism_of_Envelope_V3_Region_of_HIV_Type_1_Subtypes_A_C_and_D_from_Nairobi_Kenya","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328265"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328265/Coexpression_of_the_genes_for_platelet_derived_growth_factor_B_chain_receptor_and_macrophage_colony_stimulating_factor_1_receptor_during_monocytic_differentiation"><img alt="Research paper thumbnail of Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation" class="work-thumbnail" src="https://attachments.academia-assets.com/41818090/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328265/Coexpression_of_the_genes_for_platelet_derived_growth_factor_B_chain_receptor_and_macrophage_colony_stimulating_factor_1_receptor_during_monocytic_differentiation">Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Receptors for platelet-derived growth factor (PDGF) have not been identified previously to our kn...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Receptors for platelet-derived growth factor (PDGF) have not been identified previously to our knowledge in human myeloid cells that also produce PDGF. Here we report that phorbol ester-treated myeloid cells differentiated along the monocytic lineage express both a full-length 5.5kilobase (kb) mRNA and a predominant, truncated 4.6-kb mRNA coding for the PDGF B-chain receptor (PDGF-BR). PDGF-BR was identified in phorbol ester-differentiated myeloid cells by indirect immunofluorescence with an antibody specific to PDGF-BR. This anti-PDGF-BR was also used in immunoprecipitation studies to demonstrate that lysates of phorbol ester-differentiated myeloid cells contain PDGF-BR molecules of 37 kDa to 130 kDa. The results also show that the tandemly linked genes for PDGF-BR and the macrophage colony-stimulating factor 1 receptor are coexpressed in the phorbol ester-differentiated myeloid cells. Expression of these two receptor genes has not been shown previously in any cell type to our knowledge.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1c0d0446115293cb8ab2a7b93730d375" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818090,"asset_id":21328265,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818090/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328265"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328265"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328265; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328265]").text(description); $(".js-view-count[data-work-id=21328265]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328265; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328265']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1c0d0446115293cb8ab2a7b93730d375" } } $('.js-work-strip[data-work-id=21328265]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328265,"title":"Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation","internal_url":"https://www.academia.edu/21328265/Coexpression_of_the_genes_for_platelet_derived_growth_factor_B_chain_receptor_and_macrophage_colony_stimulating_factor_1_receptor_during_monocytic_differentiation","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818090,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818090/thumbnails/1.jpg","file_name":"Coexpression_of_the_genes_for_platelet-d20160131-9418-nkpkk4.pdf","download_url":"https://www.academia.edu/attachments/41818090/download_file","bulk_download_file_name":"Coexpression_of_the_genes_for_platelet_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818090/Coexpression_of_the_genes_for_platelet-d20160131-9418-nkpkk4-libre.pdf?1454277111=\u0026response-content-disposition=attachment%3B+filename%3DCoexpression_of_the_genes_for_platelet_d.pdf\u0026Expires=1740946885\u0026Signature=EfACDfnnMjFBk6I4xAa~kmSFMkq-F8OTs6-GzYS~Kcw-N4gVvzS50VQarNGGkg-EFXNmHaF4N6OX~81i6h70oyd8SHTKpfpJqGSOEV~7mrQ8jABQWeGSckL~nOlW78i1lVFSvfeP3XgopDH5pCiPUBg43~D0mTVGTS3x-riQoKGBUK9NtD~YhzYUb9sjUsdce5IkFGO0kuvPB4qR7x5-y7Cm1mIYlVnz6yW0qxT8-7HwStzspLmle47k2omZevnIvwBjnTVK98UwuWaZnDBGoSce544x6jU1DyBK2zNgIjHsxeB630n2zm3B157S4AEE446-CQjKUH817bjP0bNoxw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328264"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328264/Cell_type_specific_pattern_of_myc_protooncogene_expression_in_developing_human_embryos"><img alt="Research paper thumbnail of Cell-type-specific pattern of myc protooncogene expression in developing human embryos" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328264/Cell_type_specific_pattern_of_myc_protooncogene_expression_in_developing_human_embryos">Cell-type-specific pattern of myc protooncogene expression in developing human embryos</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1985</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The expression of viral oncogenes in cells transformed by acutely transforming retroviruses profo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The expression of viral oncogenes in cells transformed by acutely transforming retroviruses profoundly alters proliferation and differentiation in the target cell, suggesting that the cellular homologues of the viral oncogenes, the protooncogenes, have a role in normal cell proliferation and differentiation. To investigate the possible developmental role of protooncogenes in human embryogenesis, we have determined the spatial distribution of myc gene transcripts in early human embryos by using in situ hybridization of a labeled myc exon to thin sections. The results indicate a stage- and cell-type-specific regulation of c-myc gene expression in primarily epithelial cells of late first trimester embryos. Furthermore, the data suggest that the linkage between c-myc gene expression and cellular proliferation holds for only a restricted set of embryonic cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328264"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328264"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328264; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328264]").text(description); $(".js-view-count[data-work-id=21328264]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328264; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328264']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328264]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328264,"title":"Cell-type-specific pattern of myc protooncogene expression in developing human embryos","internal_url":"https://www.academia.edu/21328264/Cell_type_specific_pattern_of_myc_protooncogene_expression_in_developing_human_embryos","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328263"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/21328263/Induction_of_c_sis_mRNA_and_activity_similar_to_platelet_derived_growth_factor_by_transforming_growth_factor_beta_a_proposed_model_for_indirect_mitogenesis_involving_autocrine_activity"><img alt="Research paper thumbnail of Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/21328263/Induction_of_c_sis_mRNA_and_activity_similar_to_platelet_derived_growth_factor_by_transforming_growth_factor_beta_a_proposed_model_for_indirect_mitogenesis_involving_autocrine_activity">Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1986</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth fac...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor beta resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium. In addition, PDGF-regulated genes (c-fos and c-myc) were stimulated by transforming growth factor beta with delayed kinetics relative to that seen in other cell systems with direct PDGF stimulation. A model is proposed in which the monolayer mitogenicity of transforming growth factor beta is mediated by the induction of c-sis and PDGF and the subsequent autocrine stimulation of c-fos, c-myc, and other PDGF-inducible genes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328263"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328263"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328263; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328263]").text(description); $(".js-view-count[data-work-id=21328263]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328263; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328263']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=21328263]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328263,"title":"Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity","internal_url":"https://www.academia.edu/21328263/Induction_of_c_sis_mRNA_and_activity_similar_to_platelet_derived_growth_factor_by_transforming_growth_factor_beta_a_proposed_model_for_indirect_mitogenesis_involving_autocrine_activity","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328262"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328262/Integration_of_proviral_DNA_into_the_PDGF_%CE%B2_receptor_gene_in_HTLV_I_infected_T_cells_results_in_a_novel_tyrosine_kinase_product_with_transforming_activity"><img alt="Research paper thumbnail of Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity" class="work-thumbnail" src="https://attachments.academia-assets.com/41818092/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328262/Integration_of_proviral_DNA_into_the_PDGF_%CE%B2_receptor_gene_in_HTLV_I_infected_T_cells_results_in_a_novel_tyrosine_kinase_product_with_transforming_activity">Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity</a></div><div class="wp-workCard_item"><span>Oncogene</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have previously shown that noninfected human T-cell lines express the canonical 5.7 kb mRNA co...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have previously shown that noninfected human T-cell lines express the canonical 5.7 kb mRNA coding for the type b platelet-derived growth factor-receptor (PDGF breceptor), whereas HTLV-I-infected T-cell lines express a novel PDGF b-receptor mRNA of 3.8 kb. In this report, we have extended those studies to molecularly characterize the 3.8 kb PDGF b-receptor mRNA and show that it has resulted from integration of an apparently undeleted HTLV-I provirus into the PDGF b-receptor gene in an orientation enabling expression of a truncated PDGF b-receptor mRNA using the 3' HTLV-I long terminal repeat as a promoter. Further, NIH3T3 cells transfected with a plasmid containing the truncated PDGF b-receptor ORF plasmid generate colonies in soft agar with more cells per colony than untransfected cells, or cells transfected with the Tax 1 or PDGF-B (c-sis) plasmids. These results indicate that the truncated PDGF b-receptor protein acquires transforming capability and that HTLV-I-induced truncation of PDGF b-receptor may correlate with HTLV-I-associated neoplasia of human T-cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="684e30daad039b51a0be95b32cd2da85" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818092,"asset_id":21328262,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818092/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328262"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328262"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328262; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328262]").text(description); $(".js-view-count[data-work-id=21328262]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328262; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328262']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "684e30daad039b51a0be95b32cd2da85" } } $('.js-work-strip[data-work-id=21328262]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328262,"title":"Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity","internal_url":"https://www.academia.edu/21328262/Integration_of_proviral_DNA_into_the_PDGF_%CE%B2_receptor_gene_in_HTLV_I_infected_T_cells_results_in_a_novel_tyrosine_kinase_product_with_transforming_activity","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818092,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818092/thumbnails/1.jpg","file_name":"Integration_of_proviral_DNA_into_the_PDG20160131-1245-bfykth.pdf","download_url":"https://www.academia.edu/attachments/41818092/download_file","bulk_download_file_name":"Integration_of_proviral_DNA_into_the_PDG.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818092/Integration_of_proviral_DNA_into_the_PDG20160131-1245-bfykth-libre.pdf?1454277112=\u0026response-content-disposition=attachment%3B+filename%3DIntegration_of_proviral_DNA_into_the_PDG.pdf\u0026Expires=1740946885\u0026Signature=B0Q-e4xs1lb8sheNlS49uoT~MEn2Nni7JsISS77P08iAyKa2xrPFWORdN4oRtm4~qLEARTmf4cc-uD83DQP-PqSToWoX92VEG3gvJAfSLpMAcZKtNfQJPeDJQ-7eDaHDiWemqhcluSb2jAf7zgRB6xkbfstZgAknPWlCRkir~~Za~FAkStwYq3aR9mmLAimJSdvz0MjigUlkJOZpnz1A26ArxnXd9EmpaC~d-CGi8VcQm3aaGWikhwKOoLj3DrJjmsiQsTz0xEtUMaSxsawXcz-mFwkfp8EQTB-6C-fnHwqmwgKyzNeJgB9J6bd3H~R9vuvUUQ7uEm83tEIJWD-1BQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328261"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328261/Absolute_quantification_of_target_DNA_a_simple_competitive_PCR_for_efficient_analysis_of_multiple_samples"><img alt="Research paper thumbnail of Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples" class="work-thumbnail" src="https://attachments.academia-assets.com/41818119/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328261/Absolute_quantification_of_target_DNA_a_simple_competitive_PCR_for_efficient_analysis_of_multiple_samples">Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://wayne.academia.edu/AGoustin">A. Goustin</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/VladimirZachar">Vladimir Zachar</a></span></div><div class="wp-workCard_item"><span>Nucleic Acids Research</span><span>, 1993</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponen...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponential nature of DNA amplification, however, is prone to burden the experimental data with significant standard error due to the tube-to-tube inherent variations in amplification efficiency. Therefore the reliable quantitation of DNA or RNA templates by PCR necessitates introduction of an internal standard. Typically, in a series of replicates of analyzed sample the defined range of such standards is coamplified together with constant unknown amount of wildtype template (1, 2) or alternatively both templates are subjected to endpoint dilution (3). Obviously, each of the methods is based on multiple PCR reactions which are to be performed simultaneously with each of the analyzed samples. Such procedure, nevertheless, may become cumbersome when large numbers of samples are to be analyzed. A INPUT 429 bp TEMPLATE [pg] be BE 4O 429 bp 4 222 bp</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a88c044d444fa94c49fe186d1bf96065" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818119,"asset_id":21328261,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818119/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328261"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328261"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328261; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328261]").text(description); $(".js-view-count[data-work-id=21328261]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328261; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328261']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a88c044d444fa94c49fe186d1bf96065" } } $('.js-work-strip[data-work-id=21328261]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328261,"title":"Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples","internal_url":"https://www.academia.edu/21328261/Absolute_quantification_of_target_DNA_a_simple_competitive_PCR_for_efficient_analysis_of_multiple_samples","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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Goustin</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RobertCoffey4">Robert Coffey</a></span></div><div class="wp-workCard_item"><span>Nature</span><span>, 1987</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dca38447e66cbfdf9e6b0bd87b04eb31" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818088,"asset_id":21328260,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818088/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328260"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328260"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328260; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328260]").text(description); $(".js-view-count[data-work-id=21328260]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328260; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328260']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dca38447e66cbfdf9e6b0bd87b04eb31" } } $('.js-work-strip[data-work-id=21328260]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328260,"title":"Production and auto-induction of transforming growth factor-α in human keratinocytes","internal_url":"https://www.academia.edu/21328260/Production_and_auto_induction_of_transforming_growth_factor_%CE%B1_in_human_keratinocytes","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. Goustin","url":"https://wayne.academia.edu/AGoustin"},"attachments":[{"id":41818088,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41818088/thumbnails/1.jpg","file_name":"Production_and_auto-Induction_of_transfo20160131-6055-1lw9oam.pdf","download_url":"https://www.academia.edu/attachments/41818088/download_file","bulk_download_file_name":"Production_and_auto_induction_of_transfo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41818088/Production_and_auto-Induction_of_transfo20160131-6055-1lw9oam-libre.pdf?1454277111=\u0026response-content-disposition=attachment%3B+filename%3DProduction_and_auto_induction_of_transfo.pdf\u0026Expires=1740946885\u0026Signature=L5jBlaaL1mxb9DEEQqCUOZerb~RKav9wGgJs90KKDB~nw4ROhgzTr1gtWp3LwCvhIVPrQTQzNgGGLBMRXlcCuf1sd~uLy0MGVgZwiojuFbV8I3G6xayrALQRG-UfUVUFR-IQf4vEe74s4j1e0xHLcPArKgcNZJzy7SlRYS2Gs1cE3M56FE8voAEjrHltBw2PKd2-daPK7nPupokqh4y1J3Y-LTEfVFEsjJTInDUC5LXZk3F1UAW9inGjN0qXY7HGQfyT2NmLHo5YX6UGH7szxWqMOzrcd4QO9m2enwdgQgCNFTN2Isr~ZjMGwuabyj5W8UGUSFG7ntVZpgXZilKZEw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328259"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328259/Serum_alpha_2_HS_glycoprotein_is_an_inhibitor_of_the_human_insulin_receptor_at_the_tyrosine_kinase_level"><img alt="Research paper thumbnail of Serum alpha 2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level" class="work-thumbnail" src="https://attachments.academia-assets.com/41904399/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328259/Serum_alpha_2_HS_glycoprotein_is_an_inhibitor_of_the_human_insulin_receptor_at_the_tyrosine_kinase_level">Serum alpha 2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level</a></div><div class="wp-workCard_item"><span>Molecular Endocrinology</span><span>, 1993</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The insulin-dependent tyrosine kinase activity (TKA) of the insulin receptor (IR) plays an essent...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The insulin-dependent tyrosine kinase activity (TKA) of the insulin receptor (IR) plays an essential role in insulin signaling. Thus, dysregulation of IR-TKA might be an important element in the states of insulin resistance. A phosphorylated rat hepatic glycoprotein (pp63) acting as an inhibitor of IR-TK has been described. In search of the human homolog of pp63, we isolated a cDNA clone from a human liver lambda gt11 cDNA library. DNA sequence analysis reveals identity with the mRNA product of a human gene AHSG encoding a serum protein, alpha 2-Heremans Scmid-glycoprotein (alpha 2HSG), with heretofore unknown physiological function. Northern blot analysis demonstrates a 1.8-kilobase mRNA in human liver and HepG2 hepatoma cells. alpha 2HSG, purified from human serum, specifically inhibits insulin-stimulated IR autophosphorylation in vitro and in vivo as well as exogenous substrate tyrosine phosphorylation. alpha 2HSG also inhibits both insulin-induced tyrosine phosphorylation of IRS-1 and the association of IRS-1 with the p85 subunit of phosphatidylinositol-3 kinase in H-35 hepatoma cells. alpha 2HSG inhibits insulin-dependent mitogenesis, but does not affect insulin-stimulated induction of the metabolic enzyme tyrosine aminotransferase. alpha 2HSG does not compete with insulin for binding to IR. Finally, the action of alpha 2HSG is specific toward the IR-TK; its effect does not extend to insulin-like growth factor-I-stimulated TKA. Our results allow us to assign a biochemical function for human alpha 2HSG, namely regulation of insulin action at the IR-TK level.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a47dc553fac5ddfe6f5c662b04dc844d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41904399,"asset_id":21328259,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41904399/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328259"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328259"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328259; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328259]").text(description); $(".js-view-count[data-work-id=21328259]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328259; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328259']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a47dc553fac5ddfe6f5c662b04dc844d" } } $('.js-work-strip[data-work-id=21328259]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328259,"title":"Serum alpha 2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level","internal_url":"https://www.academia.edu/21328259/Serum_alpha_2_HS_glycoprotein_is_an_inhibitor_of_the_human_insulin_receptor_at_the_tyrosine_kinase_level","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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Our functional studies of human SIRT2, a homolog of the product of the yeast SIR2 gene, indicate that it plays a role in mitosis. The SIRT2 protein is a NADdependent deacetylase (NDAC), the abundance of which increases dramatically during mitosis and is multiply phosphorylated at the G 2 /M transition of the cell cycle. Cells stably overexpressing the wild-type SIRT2 but not missense mutants lacking NDAC activity show a marked prolongation of the mitotic phase of the cell cycle. Overexpression of the protein phosphatase CDC14B, but not its close homolog CDC14A, results in dephosphorylation of SIRT2 with a subsequent decrease in the abundance of SIRT2 protein. A CDC14B mutant defective in catalyzing dephosphorylation fails to change the phosphorylation status or abundance of SIRT2 protein. Addition of 26S proteasome inhibitors to human cells increases the abundance of SIRT2 protein, indicating that SIRT2 is targeted for degradation by the 26S proteasome. Our data suggest that human SIRT2 is part of a phosphorylation cascade in which SIRT2 is phosphorylated late in G 2 , during M, and into the period of cytokinesis. CDC14B may provoke exit from mitosis coincident with the loss of SIRT2 via ubiquitination and subsequent degradation by the 26S proteasome.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="076a6395f3379a5f7591bf750e302ccb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818083,"asset_id":21328258,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818083/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328258"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328258"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328258; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21328258]").text(description); $(".js-view-count[data-work-id=21328258]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21328258; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='21328258']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "076a6395f3379a5f7591bf750e302ccb" } } $('.js-work-strip[data-work-id=21328258]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":21328258,"title":"Role for Human SIRT2 NAD-Dependent Deacetylase Activity in Control of Mitotic Exit in the Cell Cycle","internal_url":"https://www.academia.edu/21328258/Role_for_Human_SIRT2_NAD_Dependent_Deacetylase_Activity_in_Control_of_Mitotic_Exit_in_the_Cell_Cycle","owner_id":34163772,"coauthors_can_edit":true,"owner":{"id":34163772,"first_name":"A.","middle_initials":null,"last_name":"Goustin","page_name":"AGoustin","domain_name":"wayne","created_at":"2015-08-23T16:54:16.127-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="21328257"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/21328257/Basal_and_Tat_transactivated_expression_from_the_human_immunodeficiency_virus_type_1_long_terminal_repeat_in_human_placental_trophoblast_rules_out_promoter_enhancer_activation_as_the_partial_block_to_viral_replication"><img alt="Research paper thumbnail of Basal and Tat-transactivated expression from the human immunodeficiency virus type 1 long terminal repeat in human placental trophoblast rules out promoter-enhancer activation as the partial block to viral replication" class="work-thumbnail" src="https://attachments.academia-assets.com/41818131/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/21328257/Basal_and_Tat_transactivated_expression_from_the_human_immunodeficiency_virus_type_1_long_terminal_repeat_in_human_placental_trophoblast_rules_out_promoter_enhancer_activation_as_the_partial_block_to_viral_replication">Basal and Tat-transactivated expression from the human immunodeficiency virus type 1 long terminal repeat in human placental trophoblast rules out promoter-enhancer activation as the partial block to viral replication</a></div><div class="wp-workCard_item"><span>Journal of General Virology</span><span>, 1994</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="80de21d092a5ea75e67c3baae003ea5d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41818131,"asset_id":21328257,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41818131/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="21328257"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="21328257"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21328257; 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