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<meta name="description" content="Search results for: ETEC"> <meta name="keywords" content="ETEC"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" 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class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="ETEC"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 9</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: ETEC</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Understanding the Prevalence and Expression of Virulence Factors Harbored by Enterotoxigenic Escherichia Coli </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Debjyoti%20Bhakat">Debjyoti Bhakat</a>, <a href="https://publications.waset.org/abstracts/search?q=Indranil%20Mondal"> Indranil Mondal</a>, <a href="https://publications.waset.org/abstracts/search?q=Asish%20K.%20Mukhopadayay"> Asish K. Mukhopadayay</a>, <a href="https://publications.waset.org/abstracts/search?q=Nabendu%20S.%20Chatterjee"> Nabendu S. Chatterjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Enterotoxigenic Escherichia coli is one of the leading causes of diarrhea in infants and travelers in developing countries. Colonization factors play an important role in pathogenesis and are one of the main targets for Enterotoxigenic Escherichia coli (ETEC) vaccine development. However, ETEC vaccines had poorly performed in the past, as the prevalence of colonization factors is region-dependent. There are more than 25 classical colonization factors presently known to be expressed by ETEC, although all are not expressed together. Further, there are other multiple non-classical virulence factors that are also identified. Here the presence and expression of common classical and non-classical virulence factors were studied. Further studies were done on the expression of prevalent colonization factors in different strains. For the prevalence determination, multiplex polymerase chain reaction (PCR) was employed, which was confirmed by simplex PCR. Quantitative RT-PCR was done to study the RNA expression of these virulence factors. Strains negative for colonization factors expression were confirmed by SDS-PAGE. Among the clinical isolates, the most prevalent toxin was est+elt, followed by est and elt, while the pattern was reversed in the control strains. There were 29% and 40% strains negative for any classical colonization factors (CF) or non-classical virulence factors (NCVF) among the clinical and control strains, respectively. Among CF positive ETEC strains, CS6 and CS21 were the prevalent ones in the clinical strains, whereas in control strains, CS6 was the predominant one. For NCVF genes, eatA was the most prevalent among the clinical isolates and etpA for control. CS6 was the most expressed CF, and eatA was the predominantly expressed NCVF for both clinical and controlled ETEC isolates. CS6 expression was more in strains having CS6 alone. Different strains express CS6 at different levels. Not all strains expressed their respective virulence factors. Understanding the prevalent colonization factor, CS6, and its nature of expression will contribute to designing an effective vaccine against ETEC in this region of the globe. The expression pattern of CS6 also will help in examining the relatedness between the ETEC subtypes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=classical%20virulence%20factors" title="classical virulence factors">classical virulence factors</a>, <a href="https://publications.waset.org/abstracts/search?q=CS6" title=" CS6"> CS6</a>, <a href="https://publications.waset.org/abstracts/search?q=diarrhea" title=" diarrhea"> diarrhea</a>, <a href="https://publications.waset.org/abstracts/search?q=enterotoxigenic%20escherichia%20coli" title=" enterotoxigenic escherichia coli"> enterotoxigenic escherichia coli</a>, <a href="https://publications.waset.org/abstracts/search?q=expression" title=" expression"> expression</a>, <a href="https://publications.waset.org/abstracts/search?q=non-classical%20virulence%20factors" title=" non-classical virulence factors"> non-classical virulence factors</a> </p> <a href="https://publications.waset.org/abstracts/112917/understanding-the-prevalence-and-expression-of-virulence-factors-harbored-by-enterotoxigenic-escherichia-coli" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/112917.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">156</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Prediction and Identification of a Permissive Epitope Insertion Site for St Toxoid in cfaB from Enterotoxigenic Escherichia coli</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20Zeinalzadeh">N. Zeinalzadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahdi%20Sadeghi"> Mahdi Sadeghi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Enterotoxigenic Escherichia coli (ETEC) is the most common cause of non-inflammatory diarrhea in the developing countries, resulting in approximately 20% of all diarrheal episodes in children in these areas. ST is one of the most important virulence factors and CFA/I is one of the frequent colonization factors that help to process of ETEC infection. ST and CfaB (CFA/I subunit) are among vaccine candidates against ETEC. So, ST because of its small size is not a good immunogenic in the natural form. However to increase its immunogenic potential, here we explored candidate positions for ST insertion in CfaB sequence. After bioinformatics analysis, one of the candidate positions was selected and the chimeric gene (cfaB*st) sequence was synthesized and expressed in E. coli BL21 (DE3). The chimeric recombinant protein was purified with Ni-NTA columns and characterized with western blot analysis. The residue 74-75 of CfaB sequence could be a good candidate position for ST and other epitopes insertion. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title="bioinformatics">bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=CFA%2FI" title=" CFA/I"> CFA/I</a>, <a href="https://publications.waset.org/abstracts/search?q=enterotoxigenic%20E.%20coli" title=" enterotoxigenic E. coli"> enterotoxigenic E. coli</a>, <a href="https://publications.waset.org/abstracts/search?q=ST%20toxoid" title=" ST toxoid"> ST toxoid</a> </p> <a href="https://publications.waset.org/abstracts/41728/prediction-and-identification-of-a-permissive-epitope-insertion-site-for-st-toxoid-in-cfab-from-enterotoxigenic-escherichia-coli" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41728.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">448</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> High Prevalence of Multi-drug Resistant Diarrheagenic Escherichia coli among Hospitalised Diarrheal Patients in Kolkata, India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Debjani%20Ghosh">Debjani Ghosh</a>, <a href="https://publications.waset.org/abstracts/search?q=Goutam%20Chowdhury"> Goutam Chowdhury</a>, <a href="https://publications.waset.org/abstracts/search?q=Prosenjit%20Samanta"> Prosenjit Samanta</a>, <a href="https://publications.waset.org/abstracts/search?q=Asish%20Kumar%20Mukhopadhyay"> Asish Kumar Mukhopadhyay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acute diarrhoea caused by diarrheagenic Escherichia coli (DEC) is one of the major public health problem in developing countries, mainly in Asia and Africa. DEC consists of six pathogroups, but the majority of the cases were associated with the three pathogropus, enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), and enteropathogenic E. coli (EPEC). Hence, we studied the prevalence and antimicrobial resistance of these three major DEC pathogroups in hospitalized diarrheal patients in Kolkata, India, during 2012-2019 with a large sample size. 8,891 stool samples were processed, and 7.8% of them was identified as DEC infection screened by multiplex PCR, in which ETEC was most common (47.7%) followed by EAEC (38.4%) and EPEC (13.9%). Clinical patient history suggested that children <5 years of age were mostly affected with ETEC and EAEC, whereas people within >5-14 years of age were significantly associated with EPEC and ETEC infections. Antibiogram profile showed a high prevalence of multidrug resistant (MDR) isolates among DEC (56.9%), in which 9% were resistant to antibiotics of six different antimicrobial classes. Screening of the antibiotic resistance conferring genes in DEC showed the presence of blaCTX-M (30.2%) in highest number followed by blaTEM (27.5%), tetB (18%), sul2 (12.6%), strA (11.8%), aadA1 (9.8%), blaOXA-1 (9%), dfrA1 (1.6%) and blaSHV (1.2%) which indicates the existence of mobile genetic elements in those isolates. Therefore, the presence of MDR DEC strains in higher number alarms the public health authorities to take preventive measures before the upsurge of the DEC caused diarrhea cases in near future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diarrheagenic%20escherichia%20coli" title="diarrheagenic escherichia coli">diarrheagenic escherichia coli</a>, <a href="https://publications.waset.org/abstracts/search?q=ETEC" title=" ETEC"> ETEC</a>, <a href="https://publications.waset.org/abstracts/search?q=EAEC" title=" EAEC"> EAEC</a>, <a href="https://publications.waset.org/abstracts/search?q=EPEC" title=" EPEC"> EPEC</a> </p> <a href="https://publications.waset.org/abstracts/143560/high-prevalence-of-multi-drug-resistant-diarrheagenic-escherichia-coli-among-hospitalised-diarrheal-patients-in-kolkata-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143560.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Development and Efficacy Assessment of an Enteric Coated Porous Tablet Loaded with F4 Fimbriae for Oral Vaccination against Enterotoxigenic Escherichia coli Infections</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Atul%20Srivastava">Atul Srivastava</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20V.%20Gowda"> D. V. Gowda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Enterotoxigenic Escherichia coli (ETEC) infection is one of the major causes contributing to the development of diarrhoea in adults and children in developing countries. To date, no preventive/treatment strategy showed promising results, which could be due to the lack of potent vaccines, and/or due to the development of resistance of ETEC to antibiotics. Therefore, in the present investigation, a novel porous Sodium Alginate (SA) tablet formulation loaded with F4 fimbriae antigen was developed and tested for efficacy against ETEC infections in piglet models. Pre-compression parameters of the powder mixes and post compression parameters of tablets have been evaluated and results were found to be satisfactory. Loading of F4 fimbrial antigens in to the tablets was achieved by inducing pores in the tablets via the sublimation of camphor followed by incubation with purified F4 fimbriae. The loaded tablets have been coated with Eudragit L100 to protect the F4 fimbriae from (a) highly acidic gastric environment; (b) proteolytic cleavage by pepsin; and (c) to promote subsequent release in the intestine. Evaluation of developed F4 fimbrial tablets in a Pig model demonstrated induction of mucosal immunity, and a signi铿乧ant reduction of F4+ E. coli in faeces. Therefore, F4 fimbriae loaded porous tablets could be a novel oral vaccination candidate to induce mucosal and systemic immunity against ETEC infections. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=porous%20tablets" title="porous tablets">porous tablets</a>, <a href="https://publications.waset.org/abstracts/search?q=sublimation" title=" sublimation"> sublimation</a>, <a href="https://publications.waset.org/abstracts/search?q=f4%20fimbriae" title=" f4 fimbriae"> f4 fimbriae</a>, <a href="https://publications.waset.org/abstracts/search?q=eudragit%20l100" title=" eudragit l100"> eudragit l100</a>, <a href="https://publications.waset.org/abstracts/search?q=vaccination" title=" vaccination"> vaccination</a> </p> <a href="https://publications.waset.org/abstracts/27290/development-and-efficacy-assessment-of-an-enteric-coated-porous-tablet-loaded-with-f4-fimbriae-for-oral-vaccination-against-enterotoxigenic-escherichia-coli-infections" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27290.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">341</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Characterization of Enterotoxigenic Escherichia coli CS6 Promoter</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mondal%20Indranil">Mondal Indranil</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhakat%20Debjyoti"> Bhakat Debjyoti</a>, <a href="https://publications.waset.org/abstracts/search?q=Mukhopadayay%20Asish%20K."> Mukhopadayay Asish K.</a>, <a href="https://publications.waset.org/abstracts/search?q=Chatterjee%20Nabendu%20S."> Chatterjee Nabendu S.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> CS6 is the prevalent CF in our region and deciphering its molecular regulators would play a pivotal role in reducing the burden of ETEC pathogenesis. In prokaryotes, most of the genes are under the control of one operon and the promoter present upstream of the gene regulates the transcription of that gene. Here the promoter of CS6 was characterized by computational method and further analyzed by 尾-galactosidase assay and sequencing. Promoter constructs and deletions were prepared as required to analyze promoter activity. The effect of different additives on the CS6 promoter was analysed by the 尾-galactosidase assay. Bioinformatics analysis done by Softberry/BPROM predicted fur, lrp, and crp boxes, -10 and -35 region upstream of the CS6 gene. The promoter construction in no promoter plasmid pTL61T showed that region -573 to +1 is actually the promoter region as predicted. Sequential deletion of the region upstream of CS6 revealed that promoter activity remains the same when -573bp to -350bp is deleted. But after the deletion of the upstream region -350 bp to -255bp, promoter expression decreases drastically to 26%. Further deletion also decreases promoter activity up to a little range. So the region -355bp to -255bp holds the promoter sequence for the CS6 gene. Additives like iron, NaCl, etc., modulate promoter activity in a dose-dependent manner. From the promoter analysis, it can be said that the minimum region lies between -254 and +1. Important region(s) lies between -350 bp to -255 bp upstream in the promoter, which might have important elements needed to control CS6 gene expression. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microbiology" title="microbiology">microbiology</a>, <a href="https://publications.waset.org/abstracts/search?q=promoter" title=" promoter"> promoter</a>, <a href="https://publications.waset.org/abstracts/search?q=colonization%20factor" title=" colonization factor"> colonization factor</a>, <a href="https://publications.waset.org/abstracts/search?q=ETEC" title=" ETEC"> ETEC</a> </p> <a href="https://publications.waset.org/abstracts/143103/characterization-of-enterotoxigenic-escherichia-coli-cs6-promoter" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143103.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">162</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Low-Cost VoIP University Solution</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Carlos%20Henrique%20Rodrigues%20de%20Oliveira">Carlos Henrique Rodrigues de Oliveira</a>, <a href="https://publications.waset.org/abstracts/search?q=Luis%20Carlos%20Costa%20Fonseca"> Luis Carlos Costa Fonseca</a>, <a href="https://publications.waset.org/abstracts/search?q=Caio%20de%20Castro%20Torres"> Caio de Castro Torres</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Gusm%C3%A3o%20Pereira"> Daniel Gusm茫o Pereira</a>, <a href="https://publications.waset.org/abstracts/search?q=Luiz%20Ricardo%20Souza%20Ripardo"> Luiz Ricardo Souza Ripardo</a>, <a href="https://publications.waset.org/abstracts/search?q=Magno%20Castro%20Moraes"> Magno Castro Moraes</a>, <a href="https://publications.waset.org/abstracts/search?q=Ana%20Paula%20Ferreira%20Costa"> Ana Paula Ferreira Costa</a>, <a href="https://publications.waset.org/abstracts/search?q=Luiz%20Carlos%20Chaves%20Lima%20Junior"> Luiz Carlos Chaves Lima Junior</a>, <a href="https://publications.waset.org/abstracts/search?q=Aurelianny%20Almeida%20da%20Cunha"> Aurelianny Almeida da Cunha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> VoIP University is a communication solution based on the IP protocol. This solution was proposed to modernize and save on communication, which required the development of Android, iOS, and Windows applications and a web service server. This solution allows integration with management system databases to create and manage a list of user extensions. VoIP UEMA was the first deployed project of VoIP University. MOS subjective voice quality test was done, and the results indicated good quality. A financial analysis revealed that annual spending on telephone bills decreased by more than 97 %. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=VoIP%20eTec" title="VoIP eTec">VoIP eTec</a>, <a href="https://publications.waset.org/abstracts/search?q=VoIP%20UEMA" title=" VoIP UEMA"> VoIP UEMA</a>, <a href="https://publications.waset.org/abstracts/search?q=VoIP%20University" title=" VoIP University"> VoIP University</a>, <a href="https://publications.waset.org/abstracts/search?q=VoIP%20Valen" title=" VoIP Valen"> VoIP Valen</a> </p> <a href="https://publications.waset.org/abstracts/182442/low-cost-voip-university-solution" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/182442.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">61</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Prospects of Milk Protein as a Potential Alternative of Natural Antibiotic</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Syeda%20Fahria%20Hoque%20Mimmi">Syeda Fahria Hoque Mimmi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many new and promising treatments for reducing or diminishing the adverse effects of microorganisms are being discovered day by day. On the other hand, the dairy industry is accelerating the economic wheel of Bangladesh. Considering all these facts, new thoughts were developed to isolate milk proteins by the present experiment for opening up a new era of developing natural antibiotics from milk. Lactoferrin, an iron-binding glycoprotein with multifunctional properties, is crucial to strengthening the immune system and also useful for commercial applications. The protein鈥檚 iron-binding capacity makes it undoubtedly advantageous to immune system modulation and different bacterial strains. For fulfilling the purpose, 4 of raw and 17 of commercially available milk samples were collected from different farms and stores in Bangladesh (Dhaka, Chittagong, and Cox鈥檚 Bazar). Protein quantification by nanodrop technology has confirmed that raw milk samples have better quantities of protein than the commercial ones. All the samples were tested for their antimicrobial activity against 18 pathogens, where raw milk samples showed a higher percentage of antibacterial activity. In addition to this, SDS-PAGE (Sodium Dodecyl Sulfate鈥揚olyacrylamide Gel Electrophoresis) was performed to identify lactoferrin in the milk samples. Lactoferrin was detected in 9 samples from which 4 were raw milk samples. Interestingly, Streptococcus pyogenes, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, Vibrio cholera, Staphylococcus aureus, and enterotoxigenic E. coli significantly displayed sensitivity against lactoferrin collected from raw milk. Only Bacillus cereus, Pseudomonas aeruginosa, Streptococcus pneumonia, Enterococcus faecalis, and ETEC (Enterotoxigenic Escherichia coli) were susceptible to lactoferrin obtained from a commercial one. This study suggested that lactoferrin might be used as the potential alternative of antibiotics for many diseases and also can be used to reduce microbial deterioration in the food and feed industry. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alternative%20of%20antibiotics" title="alternative of antibiotics">alternative of antibiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=commercially%20available%20milk" title=" commercially available milk"> commercially available milk</a>, <a href="https://publications.waset.org/abstracts/search?q=lactoferrin" title=" lactoferrin"> lactoferrin</a>, <a href="https://publications.waset.org/abstracts/search?q=nanodrop%20technology" title=" nanodrop technology"> nanodrop technology</a>, <a href="https://publications.waset.org/abstracts/search?q=pathogens" title=" pathogens"> pathogens</a>, <a href="https://publications.waset.org/abstracts/search?q=raw%20milk" title=" raw milk"> raw milk</a> </p> <a href="https://publications.waset.org/abstracts/116013/prospects-of-milk-protein-as-a-potential-alternative-of-natural-antibiotic" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116013.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">180</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Inhibition of Escherichia coli and Salmonella spp. By Traditional Phytomedicines That Are Commonly Used to Treat Gastroenteritis in Zimbabwe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Constance%20Chivengwa">Constance Chivengwa</a>, <a href="https://publications.waset.org/abstracts/search?q=Tinashe%20Mandimutsira"> Tinashe Mandimutsira</a>, <a href="https://publications.waset.org/abstracts/search?q=Jephris%20Gere"> Jephris Gere</a>, <a href="https://publications.waset.org/abstracts/search?q=Charles%20Magogo"> Charles Magogo</a>, <a href="https://publications.waset.org/abstracts/search?q=Irene%20Chikanza"> Irene Chikanza</a>, <a href="https://publications.waset.org/abstracts/search?q=Jerneja%20Vidmar"> Jerneja Vidmar</a>, <a href="https://publications.waset.org/abstracts/search?q=Walter%20Chingwaru"> Walter Chingwaru</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The use of traditional methods in the management of diarrhoea has remained a common practice among the indigenous African tribes of Southern Africa. Despite the widespread use of traditional medicines in Zimbabwe, very little research validating the activities of phytomedicines against diarrhoea, as claimed by the Shona people of Zimbabwe, has been reported. This study sought to determine the efficacies of the plants that are frequently used to treat stomach complaints, namely Dicoma anomala, Cassia abbreviata, Lannea edulis and Peltophorum africanum against Escherichia coli (an indicator of faecal contamination of water, and whose strains such as EHEC (O157), ETEC and EPEC, are responsible for a number of outbreaks of diarrhoea) and Salmonella spp. Ethanol and aqueous extracts from these plants were obtained, evaporated, dried and stored. The dried extracts were reconstituted and diluted 10-fold in nutrient broth (from 100 to 0.1 microgram/mL) and tested for inhibition against the bacteria. L. edulis exhibited the best antimicrobial effect (minimum inhibition concentration = 10 microgram/mL for both extracts and microorganisms). Runners up to L. edulis were C. abbreviata (20 microgram/mL for both microorganisms) and P. africanum (20 and 30 microgram/mL respectively). Interestingly, D. anomala, which is widely considered panacea in African medicinal practices, showed low antimicrobial activity (60 and 100 microgram/mL respectively). The high antimicrobial activity of L. edulis can be explained by its content of flavonoids, tannins, alkylphenols (cardonol 7 and cardonol 13) and dihydroalkylhexenones. The antimicrobial activities of C. abbreviata can be linked to its content of anthraquinones and triterpenoids. P. africanum is known to contain benzenoids, flavanols, flavonols, terpenes, xanthone and coumarins. This study therefore demonstrated that, among the plants that are used against diarrhoea in African traditional medicine, L. edulis is a clear winner against E. coli and Salmonella spp. Activity guided extraction is encouraged to establish the complement of compounds that have antimicrobial activities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diarrhoea" title="diarrhoea">diarrhoea</a>, <a href="https://publications.waset.org/abstracts/search?q=Escherichia%20coli" title=" Escherichia coli"> Escherichia coli</a>, <a href="https://publications.waset.org/abstracts/search?q=Salmonella" title=" Salmonella"> Salmonella</a>, <a href="https://publications.waset.org/abstracts/search?q=phytomedicine" title=" phytomedicine"> phytomedicine</a>, <a href="https://publications.waset.org/abstracts/search?q=MIC" title=" MIC"> MIC</a>, <a href="https://publications.waset.org/abstracts/search?q=Zimbabwe" title=" Zimbabwe"> Zimbabwe</a> </p> <a href="https://publications.waset.org/abstracts/52095/inhibition-of-escherichia-coli-and-salmonella-spp-by-traditional-phytomedicines-that-are-commonly-used-to-treat-gastroenteritis-in-zimbabwe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/52095.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">374</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Microbial Analysis of Street Vended Ready-to-Eat Meat around Thohoyandou Area, Vhembe District, Limpopo Province, RSA</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tshimangadzo%20Jeanette%20Raedani">Tshimangadzo Jeanette Raedani</a>, <a href="https://publications.waset.org/abstracts/search?q=Edgar%20Musie"> Edgar Musie</a>, <a href="https://publications.waset.org/abstracts/search?q=Afsatou%20Traore"> Afsatou Traore</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Street-vended meats, including chicken, pork, and beef, are popular in urban areas worldwide due to their convenience and affordability. However, these meats often pose a significant risk of foodborne diseases. The high water activity, protein content, and nearly neutral pH of meat create conditions conducive to the growth of pathogenic bacteria. Street foods, particularly meats, are frequently linked to outbreaks of foodborne illnesses due to potential contamination from improper handling and preparation. This study aimed to assess the microbial quality and safety of street-vended ready-to-eat meat sold in the Thohoyandou area. Method: The study involved collecting 168 samples of street-vended meat, split evenly between chicken (n=84) and beef (n=84), from various vendors around Thohoyandou. The samples were randomly selected and transported in sterile conditions to the Department of Food Microbiology at the University of Venda for analysis. Each 10-gram sample was cultured in selective media: MSA for Staphylococcus aureus, EMB for E. coli O157, XLD agar for Salmonella, and Sorbitol McConkey for Shigella. After initial culturing, the presumptive colonies were sub-cultured for purification and identified through Gram staining and biochemical tests, including Catalase, API 20E, Klingler Iron Agar Test, and Vitek 2 system. Antibiotic susceptibility was tested using agents such as Ampicillin, Chloramphenicol, Penicillin, Neomycin, Tetracycline, Streptomycin, and Amoxicillin. Molecular characterization was performed to identify E. coli pathotypes using multiplex PCR. Results: Out of 168 samples tested, 32 (19%) were positive for Staphylococcus spp., with the highest prevalence found in cooked chicken meat. The most common staphylococcus species identified were S. xylosus (13.2%) and S. saprophyticus (10.5%). E. coli was present in 29 (19.3%) of the samples, with the highest prevalence in fried chicken. Antibiotic susceptibility testing showed that 100% of E. coli isolates were resistant to Ampicillin, Tetracycline, and Penicillin, but 100% were susceptible to Neomycin. Staphylococcus spp. isolates were also 100% resistant to Ampicillin and 100% susceptible to Neomycin. The study detected a range of virulence genes in E. coli, with prevalence rates from 13.33% to 86.67%. The identified pathotypes included EPEC, EHEC, ETEC, EAEC, and EIEC, with many isolates showing mixed pathotypes. Conclusion: The study highlighted that the microbial quality and safety of street-vended meats in Thohoyandou are inadequate, rendering them unsafe for consumption. The presence of pathogenic microorganisms in both beef and chicken samples indicates significant risks associated with poor personal hygiene and food preparation practices. This underscores the need for improved monitoring and stricter food safety measures to prevent foodborne diseases and ensure consumer safety. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=meat" title="meat">meat</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20analysis" title=" microbial analysis"> microbial analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=street%20vendors" title=" street vendors"> street vendors</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20coli" title=" E. coli"> E. coli</a> </p> <a href="https://publications.waset.org/abstracts/190231/microbial-analysis-of-street-vended-ready-to-eat-meat-around-thohoyandou-area-vhembe-district-limpopo-province-rsa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/190231.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">27</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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