CINXE.COM

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typeof="sioc:Item foaf:Document"> <header> <span property="dc:title" content="CometAssay®" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> </header> <div class="required-fields group-hero-image-wrapper field-group-html-element"><div class="field field-name-field-hero-image field-type-et-image-field field-label-hidden"><div class="field-items"><div class="field-item even"><div class=""> <div class="hero-image-text-content"> <div class="hero-image-text-content-inner"> </div> </div> <img loading="lazy" src="https://resources.rndsystems.com/images/banners/dw_prodcat-cometassay-banner2.jpg" class="et-image-image" alt="CometAssay Single Cell Gel Electrophoresis" /> </div> </div></div></div></div><div id="product-category-wrapper" class="row group-product-category-wrapper field-group-div"><div class="col-xs-12 col-sm-8 col-md-9 col-lg-9 col-xl-9 group-left-wrapper field-group-div"><div class="group-intro field-group-div"> <div class="paragraphs-items paragraphs-items-field-introduction paragraphs-items-field-introduction-full paragraphs-items-full"> <div class="field field-name-field-introduction field-type-paragraphs field-label-hidden"><div class="field-items"><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Single Cell Gel Electrophoresis</div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div id="single-cell-gel-electrophoresis"> <p>Comet assay, or single cell gel electrophoresis assay, is a powerful technique for quantitating <a href="/products/dna-damage-and-repair-kits-reagents">DNA damage and repair</a> in eukaryotic cells and some prokaryotic cells. This technique uses cells that are embedded into agarose, placed on a slide, which is then immersed in lysis solution. After the cells are lysed, DNA is denatured and run through electrophoresis. The resulting image obtained resembles a comet that includes a distinct head and tail. The head of the comet is composed of intact DNA and the tail consists of the DNA damage. Comet assay can be run under neutral conditions to detect double-stranded breaks or alkaline conditions to detect smaller amounts of damage in DNA that include single- and double-stranded breaks.</p> <p>Due to the ease of use and ability to measure DNA damage and repair in single cells, comet assay has rapidly gained importance in the fields of genetic toxicology as well as human and environmental biomonitoring. R&D Systems offers a complete line of CometAssay products. From reagents to analysis software, we have everything you need to create standardized and reproducible results.</p> <p>Products for comet assay:</p> <ul> <li><a href="/search?keywords=CometAssay">CometAssay<sup>®</sup></a>: designed to run a standard comet assay under neutral or alkaline conditions.</li> <li><a href="/search?keywords=Electrophoresis">CometAssay<sup>®</sup> Electrophoresis System</a>: designed to consistently optimize alkaline and neutral comet assays for highly reproducible results, and to standardize electrophoresis methods between individual users and laboratories.</li> <li><a href="/search?keywords=Software">CometAssay<sup>®</sup> Analysis Software</a>: quickly analyze results created with CometAssay<sup>®</sup> products.</li> </ul> <h2>CometAssay<sup>®</sup> Products and Resources</h2> <div class="row"> <div class="col-md-6"> <ul style="list-style:none;"> <li style="padding:5px 0"><a href="#cometassay">CometAssay<sup>®</sup></a></li> <li style="padding:5px 0"><a href="#analysis">CometAssay<sup>®</sup> Analysis Software</a></li> </ul> </div> <div class="col-md-6"> <ul style="list-style:none;"> <li style="padding:5px 0"><a href="#dna-damage-resources">Additional DNA Damage and Repair Resources</a></li> </ul> </div> </div> </div> <p> </p> <p><a id="cometassay" name="cometassay"></a></p> </div></div></div></p></div> </div> </div> </div></div></div></div> </div><div class="group-basic-video field-group-div"> <div class="paragraphs-items paragraphs-items-field-basic-paragraph paragraphs-items-field-basic-paragraph-full paragraphs-items-full"> <div class="field field-name-field-basic-paragraph field-type-paragraphs field-label-hidden"><div class="field-items"><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">CometAssay®</div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><p>R&D Systems offers a complete system to run a traditional comet assay. Products include CometAssay Kits, CometSlides, CometAssay Control Cells, and a specialized CometAssay Electrophoresis System.</p> <blockquote> <p><a href="/search?keywords=CometAssay+Kit" target="_blank"><strong>CometAssay<sup>®</sup> Kit</strong></a><br /> Kits include CometAssay LMAgarose, CometAssay Lysis Solution, EDTA, and CometSlide in a <a href="/products/cometassay-single-cell-gel-electrophoresis-assay_4250-050-k" target="_blank">2-well</a> format.</p> <p><a href="/products/cometassay-electrophoresis-system_4250-050-es" target="_blank"><strong>CometAssay<sup>®</sup> Electrophoresis System II</strong></a><br /> Designed to optimize and standardize alkaline and neutral comet assays. Accommodates 2-well and <a href="/search?keywords=flare+slide" target="_blank">3-well</a> slides and maintains correct position during electrophoresis.</p> <p><a href="/products/cometassay-software_4260-000-cs" target="_blank"><strong>CometAssay<sup>®</sup> Analysis Software </strong></a><br /> Rapidly evaluate a large number of cells by automatically locating and scoring images in seconds with the ability to analyze both alkaline and neutral comet conditions</p> <p><a href="/search?keywords=slide" target="_blank"><strong>CometSlides<sup>™</sup> </strong></a><br /> Specially treated slides to promote agarose adherence and easy application of cells directly to each well. 2-well slides are available to allow for flexibility and scalability based on slide format.</p> <p><a href="/search?keywords=slide" target="_blank"><strong>FLARE<sup>™</sup> Slides </strong></a><br /> A 3-well slide that promotes adherence of LMAgarose and creates rapid and reliable analysis of large numbers of samples in a standard format.</p> <p><a href="/search?keywords=CometAssay%20starter%20kit&common_name=CometAssays&page=1" target="_blank"><strong>CometAssay<sup>®</sup> Starter Kits</strong></a><br /> Starter kits come in <a href="/products/cometassay-electrophoresis-starter-kit_4250-050-esk" target="_blank">2-well</a> format and contain the CometAssay Kit, CometAssay Control Cells, as well as the CometAssay Electrophoresis System II.</p> <p><a href="/search?keywords=cometassay+control+cells" target="_blank"><strong>CometAssay<sup>®</sup> Control Cells</strong></a><br /> Standardize comet assay runs with cryopreserved cells containing different levels of DNA damage. CometAssay Control Cells are offered for both neutral and alkaline comet assay.</p> <p><a href="/search?keywords=Silver&category=DNA%20Damage%20and%20Repair%20Kits" target="_blank"><strong>CometAssay<sup>®</sup> Silver Staining Reagents and Kit</strong></a><br /> Visualize using a standard light microscopy and stain slides permanently for archiving.</p> </blockquote> <div class="row"> <div class="col-md-6"> <p align="center"><img alt="CometAssay Single Cell Gel Electrophoresis" src="https://resources.rndsystems.com/images/site/visual-DNA-gold-stain2.jpg" /></p> <p><strong>Visualization of DNA Damage using the CometAssay Kit and SYBR Gold. </strong>Human Nalm6 cells were treated with 5 µM etoposide and visualized using the <a href="/products/cometassay-single-cell-gel-electrophoresis-assay_4250-050-k" target="_blank">CometAssay Kit (Catalog # 4250-050-K)</a> under alkali conditions. Slides were stained as recommended in the kit instructions and analyzed by microscopy. Undamaged DNA does not migrate far from the origin, while damaged DNA appears as a fluorescent comet tail.</p> </div> <div class="col-md-6"> <p align="center"><img alt="Visualization of DNA Damage using the CometAssay Silver Staining " src="https://resources.rndsystems.com/images/site/visual-DNA-silver-stain2.jpg" /></p> <p><strong>Visualization of DNA Damage using the CometAssay Silver Staining.</strong> Murin WEHI 7.1 lymphoma cells were treated with potassium permanganate and processed using the <a href="/products/cometassay-single-cell-gel-electrophoresis-assay-silver_4251-050-k" target="_blank">CometAssay Silver Kit (Catalog # 4251-050-K)</a> under alkali conditions. Slides were stained as recommended in the kit instructions and analyzed by microscopy. DNA damage is indicated by a comet tail shape and the overall migration pattern of the DNA.</p> </div> </div> <p> </p> <p><a id="cometchip" name="cometchip"></a></p> </div></div></div></p></div> </div> </div> </div><div class="field-item odd"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">CometAssay® Analysis Software</div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div class="row"> <div class="col-sm-12 col-md-8"> <p>Add the <a href="/products/cometassay-software_4260-000-cs">CometAssay<sup>®</sup> Analysis Software</a> to your CometAssay work flow and rapidly evaluate a large number of cells by automatically locating and scoring images in seconds. The software has the capability to analyze both alkaline and neutral comet conditions in low or high throughput formats. The CometAssay Analysis Software has been specially engineered to complement and operate as the computational component of R&D Systems CometAssay and CometChip Systems.</p> <p>View our <a href="https://www.youtube.com/watch?v=vEO8fxTyDVQ" target="_blank">CometAssay Analysis Software Training Video</a>.</p> </div> <div class="col-sm-12 col-md-4"><img alt="CometAssay Single Cell Gel Electrophoresis Software" src="https://resources.rndsystems.com/images/site/comet-software-4-image2-350px.jpg" /></div> </div> <p> </p> <p><a id="custom-services" name="custom-services"></a></p> </div></div></div></p></div> </div> </div> </div><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Additional DNA Damage Resources </div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div> <ul> <li><a href="/products/cometassay-assay-principle">CometAssay Assay Principle</a></li> <li><a href="/advances-high-throughput-dna-damage-detection-using-cometassay-and-cometchip-technologies">DNA Damage Detection Using CometAssay<sup>®</sup> and CometChip<sup>®</sup> Technologies</a></li> <li><a href="/resources/posters/choosing-right-model-system-hepatotoxicity-comparison-3-d-liver-organoids-hepg2">Choosing the Right Model System for Hepatotoxicity </a></li> <li><a href="https://resources.rndsystems.com/images/site/BR_CometAssay_System_STRY0063355-web.pdf" target="_blank">CometAssay System Brochure </a></li> <li><a href="https://resources.rndsystems.com/images/site2/rnd_comet-analysis-software-br.pdf" target="_blank">CometAssay Analysis Software Brochure </a></li> <li><a href="https://www.youtube.com/watch?v=vEO8fxTyDVQ" target="_blank">CometAssay Analysis Software Training Video </a></li> <li><a href="/resources/posters/regulation-cell-cycle-dna-damage-induced-checkpoint-activation">Regulation of the Cell Cycle & DNA Damage-Induced Checkpoint Activation </a></li> <li><a href="/products/dna-damage-and-repair-kits-reagents">DNA Damage and Repair Kits & Reagents</a></li> <li><a href="/search?category=DNA%20Damage%20and%20Repair%20Kits">All DNA Damage and Repair Products </a></li> <li><a href="/blog/how-keep-calm-and-monitor-cell-stress-death-and-behavior">How to Keep Calm and Monitor Cell Stress, Death, and Behavior</a></li> </ul> </div> </div></div></div></p></div> </div> </div> </div></div></div></div> </div></div><div class="col-xs-12 col-sm-4 col-md-3 col-lg-3 col-xl-3 group-featured-content-wrapper field-group-div"><h2 class="field-group-html-element"><div class="field 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class="field-item even"><a href="https://resources.rndsystems.com/images/site/BR_DNA_Damage_STRY0044835-web.pdf" target="_blank">DNA Damage Brochure</a></div></div></div></h4><div class="required-fields group-img-wrapper field-group-html-element"><div class="field field-name-field-img field-type-et-image-field field-label-hidden"><div class="field-items"><div class="field-item even"><div class=""> <div class="hero-image-text-content"> <div class="hero-image-text-content-inner"> </div> </div> <img loading="lazy" src="https://resources.rndsystems.com/images/site/DNA-damage-feature-750x471.jpg" class="et-image-image" alt="DNA Damage Brochure" /> </div> </div></div></div></div><p class="group-featured-content-text field-group-html-element"><div class="field field-name-field-featured-content-text field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Learn how Bio-Techne’s unparalleled product selection is making it easy to probe DNA damage in your model system.</div></div></div></p><div class="group-learn-more-wrapper field-group-html-element btn btn-tertiary"><div class="field field-name-field-learn-more field-type-link-field field-label-hidden"><div class="field-items"><div class="field-item even"><a href="https://resources.rndsystems.com/images/site/BR_DNA_Damage_STRY0044835-web.pdf" target="_blank">Download Brochure</a></div></div></div></div></div></div> </div> </div> </div><div class="field-item odd"><div class="entity entity-paragraphs-item paragraphs-item-featured-content" about="" typeof=""> <div class="content"> <div class="collapsible group-featured-content field-group-div m-featured-product speed-fast effect-none"> <h3><span class="field-group-format-toggler">Featured Content</span></h3> <div class="field-group-format-wrapper" style="display: block;"><h4 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-featured-title field-type-link-field field-label-hidden"><div class="field-items"><div class="field-item even"><a href="https://www.rndsystems.com/resources/posters/choosing-right-model-system-hepatotoxicity-comparison-3-d-liver-organoids-hepg2">Choosing the Right Model System for Hepatotoxicity</a></div></div></div></h4><div class="required-fields group-img-wrapper field-group-html-element"><div class="field field-name-field-img field-type-et-image-field field-label-hidden"><div class="field-items"><div class="field-item even"><div class=""> <div class="hero-image-text-content"> <div class="hero-image-text-content-inner"> </div> </div> <img loading="lazy" src="//resources.rndsystems.com/images/site/poster-hepatotoxicity-feature-750px.jpg" class="et-image-image" alt="Measuring DNA damage with single cell gel electrophoresis" /> </div> </div></div></div></div><p class="group-featured-content-text field-group-html-element"><div class="field field-name-field-featured-content-text field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">This poster shows how the CometAssay can be used to measure DNA damage for Hepatotoxicity. </div></div></div></p><div class="group-learn-more-wrapper field-group-html-element btn btn-tertiary"><div class="field field-name-field-learn-more field-type-link-field field-label-hidden"><div class="field-items"><div class="field-item even"><a href="https://www.rndsystems.com/resources/posters/choosing-right-model-system-hepatotoxicity-comparison-3-d-liver-organoids-hepg2">Download the Poster</a></div></div></div></div></div></div> </div> </div> </div><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-featured-content" about="" typeof=""> <div class="content"> <div class="collapsible group-featured-content field-group-div m-featured-product speed-fast effect-none"> <h3><span class="field-group-format-toggler">Featured Content</span></h3> <div class="field-group-format-wrapper" style="display: block;"><h4 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-featured-title field-type-link-field field-label-hidden"><div class="field-items"><div class="field-item even"><a href="https://www.youtube.com/watch?v=vEO8fxTyDVQ">CometAssay<sup>®</sup> Analysis Software Training Video</a></div></div></div></h4><div class="required-fields group-img-wrapper field-group-html-element"><div class="field field-name-field-img field-type-et-image-field field-label-hidden"><div class="field-items"><div class="field-item even"><div class=""> <div class="hero-image-text-content"> <div class="hero-image-text-content-inner"> </div> </div> <img loading="lazy" src="https://resources.rndsystems.com/images/site/comet-software-feature-750px.jpg" class="et-image-image" alt="CometAssay Training Video" /> </div> </div></div></div></div><p class="group-featured-content-text field-group-html-element"><div class="field field-name-field-featured-content-text field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Automate your comet analysis with our CometAssay Analysis Software. Learn how to analyze thousands of comets in minutes. </div></div></div></p><div class="group-learn-more-wrapper field-group-html-element btn btn-tertiary"><div class="field field-name-field-learn-more field-type-link-field field-label-hidden"><div class="field-items"><div class="field-item even"><a href="https://www.youtube.com/watch?v=vEO8fxTyDVQ">View Software Training Video </a></div></div></div></div></div></div> </div> </div> </div></div></div></div> </div></div> </article> </section> <section id="block-block-26" class="block block-block clearfix">  <div class="embeddedServiceInvitation" id="snapins_invite" aria-live="assertive" role="dialog" aria-atomic="true" style="display: none;"> <div class="embeddedServiceInvitationHeader" aria-labelledby="snapins_titletext" aria-describedby="snapins_bodytext" style="height: 48px;"> <img id="embeddedServiceAvatar" /> <span class="embeddedServiceTitleText" id="snapins_titletext">Need help?</span> <button type="button" id="closeInvite" 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