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GENEWIZ from Azenta | Difficult Template Sequencing
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padding: 0; } .sanger-new-container { background: #f0f0f0; padding: 30px 0; margin-top: 44px; } .sanger-new-container h1 { margin: 0; color: #000000; } .sanger-new-content { width: 1170px; margin: 0 auto; } .sanger-new-content p { color: #000000; } .sanger-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #sanger-content-box-left { width: 60%; text-align: left; } #sanger-content-box-right { width: 39%; text-align: right; } .sanger-content-box img { width: 400px; } .sanger-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .sanger-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .sanger-new-container { text-align: center; margin-top: 0; } .sanger-new-content { width: 90%; margin: 0 auto; } .sanger-new-content { display: inline-block; } #sanger-content-box-right, 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With GENEWIZ Difficult Template sequencing from Azenta Life Sciences, save at least 1 day in the sequencing process, and streamline your research. </p> <p>Compatible with various DNA types including PCR products, plasmids, bacterial colonies, and glycerol stocks, our service eliminates the need for additional templates, providing unparalleled convenience and efficiency for your breakthrough projects. </p> <br /> <a href="https://clims4.genewiz.com/RegisterAccount/Login?returnUrl=https://clims4.genewiz.com/SangerSequencing?lobId=1&serviceId=101&_ga=2.215942615.1180471606.1662366816-1790848659.1652957837&_gac=1.82883940.1658826966.Cj0KCQjwof6WBhD4ARIsAOi65agEJXGs-1yLwHW3W7_1mFab4et5K8CpoFzKVW1g098Ry5NVV6K6c-0aAjQOEALw_wcB"><button>Place an Order</button></a> </div> <div class="sanger-content-box" id="sanger-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02FKw2n0" target="_blank"><img alt="" src="/-/media/11038-D_PEZ-DirectColonySeq_WebBanRight.ashx?la=en&hash=9E030C709843447C76717D11FCC9D4A59384D0CC" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner { width: 1170px; background-image: url('/-/media/11034-D_Low-Conc-Plasmids_WebBanFull-Med_Background.ashx'); background-size: cover; text-align: center; display: flex; padding: 20px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 0%; padding: 10px; } #banner-right { width: 99%; padding: 0px; } #banner-right h2 { text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 10px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #FFFFFF; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px; font-weight: regular; } #banner-right button:hover { cursor: pointer; transform: scale(1.1); } #banner-right button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner { width: 95%; height: auto; background: #6959a1; display: inline-block; } #banner-left { text-align: center; width: 100%; } #banner-right { text-align: center; width: 100%; } #banner-right h2 { text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 45px; margin: 0; margin-bottom: 10px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner"> <div id="banner-left"> </div> <div id="banner-right"> <div id="banner-right h3"> <h3>Sanger sequencing for low yield plasmid samples</h3> <div id="banner-right h2"> <h2>Low Concentration? No Problem.</h2> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02kXXzr0" target="_blank"><button>LEARN MORE</button></a> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Alternative Protocols for Difficult Templates</h2> <p style="text-align: left;">Template examples that may benefit from the alternative protocols include:</p> <ul> <li style="text-align: left;"><strong>GC-rich:</strong> A template of > 60% GC content or high GC-content concentrated in a small region</li> <li style="text-align: left;"><strong>Hairpin Structures:</strong> A sequence containing two inverted repeats, separated by at least three nucleotides</li> <li style="text-align: left;"><strong>Repeats:</strong> Certain di- or tri-nucleotide repeats</li> <li style="text-align: left;"><strong>Motifs causing band compressions:</strong> Mainly 5'-YGN1-2AR motifs, (Y: pyrimidine; R: purine; N: any base)</li> <li style="text-align: left;"><strong>Bacterial Artificial Chromosomes (BAC):</strong> Any construct larger than 50 kb.</li> </ul> <p> </p> <h2 class="container">Template Examples</h2> <h3 class="container">Case 1: Small Hairpin RNA (<span style="text-transform: none;">shRNA</span>) Template Sequencing </h3> <p style="text-align: left;">The small hairpin RNA templates used in RNAi studies frequently present challenges in sequencing such as early termination or ambiguity, caused by the double-stranded or hairpin structures. Our proprietary RNAi Protocol produces superior results for our customers, saving time and cost. For a comparison, see the chromatogram below: </p> <p style="text-align: center;"> <img alt="Small Hairpin RNA Template Sequencing" src="/-/media/Images/Services/GA/charts/rnai01.ashx?la=en&hash=23EC587495AD9CF10FB597824386FEB9BF1794B9" style="height: 246px; width: 480px;" class="img-responsive center-block" /> </p> <p> </p> <h3>Case 2: Repeat / GC rich Template Sequencing</h3> <p style="text-align: left;">Repeat/GC-rich templates have special secondary structures and melting temperatures. DNA containing high GC content and hairpin structures are relatively more difficult to denature and very challenging to sequence. </p> <p style="text-align: left;">Our proprietary Hairpin and GC-rich protocols produce superior results, with strong signals and uniform peak heights at the GC region. </p> <p style="text-align: center;"> <img alt="Repeat / GC Rich Template Sequencing" src="/-/media/Images/Services/GA/charts/rnai02.ashx?la=en&hash=8163BC34D17DC997C651744DAAC34332699CA1C0" style="height: 250px; width: 480px;" class="img-responsive center-block" /> </p> </div> <br /> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="process-v1 Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-10"> <div class="container"> <h3>BAC-end Sequencing for Constructs Greater than 50 kb</h3> <p style="text-align: left;"> Large constructs such as Bacterial Artificial Chromosomes (BACs), cosmids, fosmids, and BACmids, pose a challenge to traditional Sanger sequencing protocols due to the size and interference of the non-target DNA in the template. GENEWIZ's proprietary BAC protocol to overcome the difficulty of sequencing directly from these large constructs can help deliver greater success and longer read lengths for large constructs compared to the standard protocol. </p> <p style="text-align: left;">Please contact our Technical Support team for more information on BAC protocol's: </p> <h3> <a href="mailto:DNASeq@genewiz.com"><em class="fa fa-envelope fa-icons blue-text"></em></a> <a href="mailto:DNASeq@genewiz.com">Email</a> | <em class="fa fa-phone fa-icons blue-text"></em> Phone (1-877-GENEWIZ (436-3949), Ext. 2) | <a target="_blank" onclick="Velaro.Engagement.LoadPopoutChat()"><em class="fa fa-comment fa-icons blue-text"></em> Live Chat</a></h3> <div><a target="_blank" onclick="Velaro.Engagement.LoadPopoutChat()"><br /> </a></div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <h3>From Base Pairs to Breakthroughs: Understanding<br /> Sanger and Oxford Nanopore Sequencing</h3> <p>Monday, September 16, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02JnkNS0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>FEATURES & BENEFITS</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Advanced Technology</strong><br /> GENEWIZ provides our difficult template sequencing service (see above) using modified protocols, developed in-house, specifically for success with difficult templates. Years of use have confirmed that these alternative protocols deliver best-in-class results. <br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Individual Consultation</strong><br /> GENEWIZ provides individualized scientific consultation and customized solutions for your specific DNA sequencing needs, through our Technical Support team. <br /> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Technical Support</strong><br /> Our Technical Support team, staffed by multiple Ph.D. scientists with a friendly, problem-solving spirit, is available to advise and assist you at no charge Monday - Friday, 8AM - 8PM Eastern. GENEWIZ Technical Support also posts results on Saturdays, so research progress can continue forward over the weekend. <br /> </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #ffffff; padding: 0; margin: 0;margin-top: -20px;"> <h2 style="border-bottom: 1px solid #f1f0f0;">TECHNICAL RESOURCES</h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/ONT-Blog.ashx?h=160&w=220&la=en&hash=AFF32CFD100B581BA8F5F09D2DF7011B7407F301" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing</h3> <p>Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information. <br /> <a href="https://www.azenta.com/learning-center/blog/ensure-complete-plasmid-validation-your-first-attempt-oxford-nanopore-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Webinar--Sanger-ONT.ashx?h=160&w=220&la=en&hash=B46A4AA56DE351DD45E75213BD6FD1AA5AB8F799" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing</h3> <p>While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs. <br /> <a href="https://web.genewiz.com/workshop/base-pairs-to-breakthroughs" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/qPCR-dPCR-Blog.ashx?h=160&w=220&la=en&hash=E76BF29417DDCA4C25770741C98732CFC291673F" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | NGS, qPCR, dPCR, or Sanger Sequencing: An Assay Selection Guide</h3> <p>Today’s molecular biologists have many options to interrogate their genome or transcriptome of interest. Next generation sequencing (NGS), quantitative PCR (qPCR), digital PCR (dPCR), and Sanger sequencing are used widely in genomics; however, choosing the best tool for your project isn’t always intuitive. To learn more about the method best suited for your project goals, use our interactive selection guide as well as the practical information about PCR + Sanger, qPCR, dPCR, and NGS approaches below. <br /> <a href="https://www.azenta.com/learning-center/blog/ngs-qpcr-or-sanger-sequencing-assay-selection-guide" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <p> </p> </div> </div> </div> <!--=== Process v1 ===--> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> <p>*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. 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