WA Proteomics Facility | CMCA

<!DOCTYPE html>     <html class="no-js" lang="en">  <head> <link href="/research/-/media/base-themes/core-libraries/styles/optimized-min.css?t=20230527T093314Z" rel="stylesheet"><link href="/research/-/media/base-themes/main-theme/styles/optimized-min.css?t=20230527T093320Z" rel="stylesheet"> <title>WA Proteomics Facility | CMCA | UWA</title> <link rel="canonical" href="https://www.uwa.edu.au/cmca/wa-proteomics-facility" /> <meta property="og:title" content="WA Proteomics Facility"><meta property="og:url" content="https://www.uwa.edu.au/cmca/wa-proteomics-facility"> <meta property="twitter:title" content="WA Proteomics Facility"><meta property="twitter:card" content="summary_large_image"> <meta name="viewport" content="width=device-width, initial-scale=1" /> <meta name="itemid" content="05177626-788E-476E-9FD9-5E2FB7F6FB68"> <meta name="version" content="4"> <meta name="lang" content="en"> <meta name="site" content="CentreforMicroscopyCharacterisationandAnalysis"> <link href="/Theme-SXA/dist/masterbrand/main.css?v=638643367870000000" rel="stylesheet" /> <link href="https://static.weboffice.uwa.edu.au/visualid/devcore/sitecore/css/navigation.css" rel="stylesheet" />  <script>(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-N397MQR');</script>  </head> <body class="default-device bodyclass">  <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-N397MQR" height="0" width="0" style="display:none;visibility:hidden"></iframe> </noscript>  <div id="wrapper">  <header> <div id="header" class="grid-container"> <div class="component container grid-container cell full" id="uwa-header__row-primary" > <div class="component-content grid-x grid-padding-x"> <div class="component image file-type-icon-media-link medium-2 cell" id="uwa-header__container-logo"> <div class="component-content"> <div class="uwa-nav__logo"> <a href="https://www.uwa.edu.au/"> <img src="/cmca/-/media/project/uwa/uwa/masterbrand/core-elements/logo-uwacrest-white.svg?iar=0&hash=688B7CB016417780AAC3091D42ED0619" alt="The University of Western Australia" /> </a> </div> </div> </div> <div class="component navigation medium-2 cell" id="uwa-header__container-global"> <div class="component-content">  <div class="uwa-nav__links"> <button class="_menu">All UWA</button> <div id="uwa-nav_all-uwa" class="uwa-nav__menu _megamenu"> <div class="_inner"> <div class="_title"> <h2><a href="https://www.uwa.edu.au/" rel="noopener noreferrer" target="_self">The University of Western Australia</a></h2> <p>Shaping the next generation of global leaders.</p> </div> <div class="_links"> <ul> <li> <a href="https://www.uwa.edu.au/study-at-uwa">Study</a> <ul> <li> <a href="https://www.uwa.edu.au/study-at-uwa/courses">Courses and study areas</a> </li> <li> <a href="https://www.uwa.edu.au/study-at-uwa/study-options">Study options and advice</a> </li> <li> <a href="https://www.uwa.edu.au/study-at-uwa/how-to-apply">How to apply</a> </li> <li> <a href="https://www.uwa.edu.au/study-at-uwa/fees-and-scholarships">Fees and scholarships</a> </li> </ul> </li> <li> <a href="https://www.uwa.edu.au/life-at-uwa-old">Life at UWA</a> </li> <li> <a href="https://www.uwa.edu.au/partners-and-community">Partners and community</a> <ul> <li> <a href="https://www.uwa.edu.au/partners-and-community/partnerships">Industry partnerships</a> </li> <li> <a href="https://www.uwa.edu.au/partners-and-community/community-engagement">Community engagement</a> </li> <li> <a href="https://www.uwa.edu.au/partners-and-community/global-engagement">Global engagement</a> </li> <li> <a href="https://www.uwa.edu.au/partners-and-community/our-alumni">Our alumni</a> </li> <li> <a href="https://www.uwa.edu.au/partners-and-community/convocation">Convocation</a> </li> <li> <a href="https://www.uwa.edu.au/partners-and-community/giving-to-uwa">Giving</a> </li> </ul> </li> <li> <a href="https://www.uwa.edu.au/research">Our research</a> <ul> <li> <a href="https://www.uwa.edu.au/research/strengths">Research strengths</a> </li> <li> <a href="https://www.uwa.edu.au/research/reputation-governance">Reputation and governance</a> </li> <li> <a href="https://www.uwa.edu.au/research/research-degrees">Research degrees</a> </li> <li> <a href="https://www.uwa.edu.au/research/institutes-centres">Institutes and centres</a> </li> <li> <a href="https://www.uwa.edu.au/research/infrastructure">Infrastructure</a> </li> <li> <a href="https://www.uwa.edu.au/research/connect">Connect</a> </li> </ul> </li> <li> <a href="https://www.uwa.edu.au/about">About us</a> <ul> <li> <a href="https://www.uwa.edu.au/about/our-history">Our history</a> </li> <li> <a href="https://www.uwa.edu.au/about/rankings-and-reputation">Reputation</a> </li> <li> <a href="https://www.uwa.edu.au/about/leadership-and-governance">Leadership and governance</a> </li> <li> <a href="https://www.uwa.edu.au/about/locations">Our campus</a> </li> <li> <a href="https://www.uwa.edu.au/about/schools">Schools</a> </li> <li> <a href="https://www.uwa.edu.au/about/working-at-uwa">Working at UWA</a> </li> <li> <a href="https://www.uwa.edu.au/about/contact-us">Contact us</a> </li> </ul> </li> <li> <a href="https://www.uwa.edu.au/students" rel="noopener noreferrer" target="_self">Current students</a> <ul> </ul></li> <li> <a href="https://intranet.uwa.edu.au/" rel="noopener noreferrer" target="_self">Staff</a> <ul> </ul></li> <li> <a href="https://www.uwa.edu.au/news" rel="noopener noreferrer" target="_self">News</a> <ul> </ul></li> <li> <a href="https://giving.uwa.edu.au/" rel="noopener noreferrer" target="_self">Giving</a> <ul> </ul></li> <li> <a href="https://www.uwa.edu.au/study/events" rel="noopener noreferrer" target="_self">Events</a> <ul> </ul></li> </ul> </div> </div> </div> </div>  </div> </div> <div class="component plain-html medium-2 cell" id="uwa-header__container-menu"> <div class="component-content"> <div class="uwa-nav__links"> <button id="uwa-nav__mobile" class="_icon" data-target="uwa-nav__menu-mobile">Menu</button> </div> </div> </div> <div class="component search-form medium-2 cell" id="uwa-header__container-search"> <div class="component-content"> <div class="uwa-nav__links"> <button id="uwa-nav__search" class="_icon" data-target="uwa-nav__menu-search">Search</button> <div id="uwa-nav__menu-search" class="uwa-nav__menu _megamenu _search"> <div class="_inner"> <div class="_title"> <h2><a>How can we help you?</a></h2> </div> <div class="_form"> <form method="GET" action="https://www.search.uwa.edu.au/s/search.html" data-suggestion-params="collection=uwa~sp-search" id="uwa-nav__form-search"> <input type="text" name="query" placeholder="Search courses, news, people"><input type="hidden" name="collection" value="uwa~sp-search" /><button type="reset">Clear</button> <button type="submit">Search</button> </form> </div> <div class="_links"> <ul id="uwa-nav__search-suggestions"> <li><strong>Suggestions</strong> <ul></ul> </li> <li class="_results" aria-live"polite"=""><strong>Did you mean...</strong> <ul id="uwa-nav__search-suggestions-results"></ul> </li> </ul> </div></div> </div> </div> </div> </div> </div> </div> <div class="component container grid-container cell full" id="uwa-header__row-secondary" > <div class="component-content grid-x grid-padding-x"> <div class="component plain-html medium-4 cell" id="uwa-header__container-title"> <div class="component-content"> <h1>Centre for Microscopy Characterisation and Analysis</h1> </div> </div> <div class="component navigation medium-8 cell" id="uwa-header__container-site"> <div class="component-content"> <div id="uwa-nav__site" class="uwa-nav__links"> <ul> <li> <a href="/cmca/metabolomics-australia">Metabolomics Australia (UWA)</a> </li> <li> <button class="_menu">Microscopy and Analysis</button> <div class="uwa-nav__menu"> <div class="_title"> <h2><a href="/cmca/microscopy-and-analysis">Microscopy and Analysis</a></h2> </div> <div class="_links"> <ul> <li> <a href="/cmca/microscopy-and-analysis/flow-cytometry">Flow Cytometry</a> </li> <li> <a href="/cmca/microscopy-and-analysis/icp-ms">ICP-MS</a> </li> <li> <a href="/cmca/microscopy-and-analysis/nanosims">NanoSIMS</a> </li> <li> <a href="/cmca/microscopy-and-analysis/nmr">Nuclear Magnetic Resonance (NMR) Spectroscopy</a> </li> <li> <a href="/cmca/microscopy-and-analysis/optical-microscopy">Optical Microscopy</a> </li> <li> <a href="/cmca/microscopy-and-analysis/sample-preparation">Sample Preparation</a> </li> <li> <a href="/cmca/microscopy-and-analysis/sem">Scanning Electron Microscopy (SEM)</a> </li> <li> <a href="/cmca/microscopy-and-analysis/lg-sims">LG-SIMS</a> </li> <li> <a href="/cmca/microscopy-and-analysis/spm">Scanning Probe Microscopy (SPM)</a> </li> <li> <a href="/cmca/microscopy-and-analysis/tem">Transmission Electron Microscopy (TEM)</a> </li> <li> <a href="/cmca/microscopy-and-analysis/xrm">X-ray Microscopy (XRM)</a> </li> </ul> </div> <div class="_promo"> <ul> </ul> </div> </div> </li> <li> <button class="_menu">WA National Imaging Facility</button> <div class="uwa-nav__menu"> <div class="_title"> <h2><a href="/cmca/wa-national-imaging-facility">WA National Imaging Facility</a></h2> </div> <div class="_links"> <ul> <li> <a href="/cmca/wa-national-imaging-facility/nif-animals-plants-and-materials-imaging">NIF Animals Plants and Materials Imaging</a> </li> <li> <a href="/cmca/wa-national-imaging-facility/nif-human-imaging">NIF Human Imaging</a> </li> <li> <a href="/cmca/wa-national-imaging-facility/nif-radiopharmaceuticals">NIF Radiopharmaceuticals</a> </li> </ul> </div> <div class="_promo"> <ul> </ul> </div> </div> </li> <li> <a href="/cmca/wa-proteomics-facility">WA Proteomics Facility</a> </li> </ul> <button id="uwa-nav__mobile" class="_icon" data-target="uwa-nav__menu-mobile">Menu</button> </div> </div> </div> </div> </div> <div class="component breadcrumb navigation-title cell full" id="uwa-header__row-tertiary"> <div class="component-content"> <nav> <ol> <li class="breadcrumb-item home uwa-home"> <a title="UWA" href="https://www.uwa.edu.au/">UWA</a> </li> <li class="breadcrumb-item home"> <a title="Home" href="/cmca">Home</a> <span class="separator" role="presentation"></span> </li> <li class="breadcrumb-item last"> <a title="WA Proteomics Facility" href="/cmca/wa-proteomics-facility">WA Proteomics Facility</a> <span class="separator" role="presentation"></span> </li> </ol> </nav> </div> </div> </div> </header>   <main> <div id="content" class="grid-container full"> <div class="component promo small-12 cell"> <div class="component-content"> <div class="hero"><div class="hero-bkg"> <div class="hero-image"> <img src="/cmca/-/media/project/uwa/uwa/cmca/proteomics---hero_1920x432.png?w=1440&hash=450D01CAB365A95300E03807C4C020FE" alt="Proteomics team" sizes="(max-width: 320px) 320px, (max-width: 480px) 480px, 1440px" srcset="/cmca/-/media/project/uwa/uwa/cmca/proteomics---hero_1920x432.png?w=320&hash=13B78C912C8AB8E7CA1E9B84FC30AE1B 320w,/cmca/-/media/project/uwa/uwa/cmca/proteomics---hero_1920x432.png?w=480&hash=0C1BE753F38EAF76056DB78B6EE6F248 480w,/cmca/-/media/project/uwa/uwa/cmca/proteomics---hero_1920x432.png?w=1440&hash=450D01CAB365A95300E03807C4C020FE 1440w" /> </div> </div><div class="hero-grid-container"><div class="hero-content-panel"><div class="hero-content"><div class="hero-copy"><h1 class="hero-title"><span class="hero-subheading">WA Proteomics Facility</span></h1><div class="hero-description field-hero-description"><p>Cutting edge proteomics services delivered through our world class expertise and state of the art instrumentation.</p></div><div class="hero-btn-container"></div></div></div></div></div></div> </div> </div> <div class="component container grid-container small-12 cell component-margin-bottom--small" > <div class="component-content grid-x grid-padding-x"> <div class="component component-heading component-heading--section small-12 cell container-padding--bottom-small"> <div class="component-content"> <h2 class="section-title field-title">About us</h2> </div> </div> <div class="component rich-text small-12 cell"> <div class="component-content"> <p>The WA Proteomics Facility is a node of Proteomics Australia based at CMCA. Services are delivered through a joint venture between The University of Western Australia and Proteomics International.</p> <p>Proteins lie at the heart of all biology. We offer cutting edge services for the analysis, identification and quantitation of proteins as well as specialist services in measuring protein synthesis and degradation rates. At Proteomics Western Australia we offer both deep expertise and a user friendly environment to help you design and execute your studies successfully while we deliver world class data and analysis on WA's state of the art infrastructure. Our enduring aim is to build successful collaborations with the life sciences sector to contribute to Australia’s health, environmental, economic and social wellbeing.</p> <p>We can also offer support to researchers in the form of grant writing assistance and utilisation of the facility generally qualifies as an in-kind contribution from NCRIS and Bio Platforms Australia, helping your grant applications be the most competitive they can.</p> </div> </div> </div> </div> <div class="component container grid-container initial-12 cell component-margin-bottom--small" > <div class="component-content grid-x grid-padding-x"> <div class="component rich-text small-12 cell"> <div class="component-content"> <img alt="NCRIS and Bioplatforms Australia logos" src="/cmca/-/media/project/uwa/uwa/cmca/logos/ncris-and-bio.png" /> </div> </div> </div> </div> <div class="component container grid-container small-12 cell container-padding--bottom-small" > <div class="component-content grid-x grid-padding-x"> <div class="component image-liftout image-liftout--overlap small-12 cell component-margin-bottom--none"> <div class="component-content"> <div class="image-liftout-container"><figure class="image-liftout__column image-liftout__column--left"><div class="image-liftout__image-container"><div class="image-liftout__image-asset field-promoicon"><img src="/cmca/-/media/project/uwa/uwa/cmca/rich-text/wa-proteomics-facility/proteomics---our-sevices_800x535.png?h=534&iar=0&w=800&hash=015FD313D2D3FD913E99C4C9990A1D5A" alt="Female staff member of Proteomics" width="800" height="534" loading="lazy" data-variantitemid="{8912A5B6-626C-45B9-A32F-90D934606854}" data-variantfieldname="PromoIcon" /></div></div></figure><div class="image-liftout__column image-liftout__column--right"><div class="image-liftout__content-container grid-x"><h2 class="image-liftout__content-heading">Our services</h2><div class="image-liftout__content-asset field-promotext2"><p>The services offered by the WA Proteomics Facility focus on:</p> <ul> <li>Protein identification to provide evidence to link protein to the gene that encodes it</li> <li>Label free quantitation to reveal the identity and abundance of proteins in a complex sample</li> <li>Targeted proteomics to selectively quantitate specific proteins of interest</li> <li>Flux proteomics to measure the synthesis and degradation rates of proteins</li> </ul></div><div class="btn btn--primary field-promolink"><a data-variantfieldname="PromoLink" href="/cdn-cgi/l/email-protection#6515170a11000a080c0616251012044b0001104b0410" data-variantitemid="{8912A5B6-626C-45B9-A32F-90D934606854}">Contact us</a></div></div></div></div> </div> </div> </div> </div> <div class="component container grid-container small-12 cell component-margin-bottom--none" > <div class="component-content grid-x grid-padding-x"> <div class="component tabs small-12 cell tabs--default js-tabs-scroll"> <div class="component-content"> <div class="tabs-inner"> <ul role="tablist" class="tabs-heading" aria-label=""> <li style="order:1" > <button tabindex="-1" role="tab" class="tab__button" aria-controls="panel-0F029030-1B88-438D-92FE-611BBEF2116F" aria-selected="false" id="tab-0F029030-1B88-438D-92FE-611BBEF2116F" > Label Free Quantitative Proteomics <div style="font-weight: 700; height: 0; overflow: hidden;" aria-hidden="true"> Label Free Quantitative Proteomics</div> </button> </li> <li style="order:3" > <button tabindex="-1" role="tab" class="tab__button" aria-controls="panel-56C7AC42-F7D6-4AD4-A13F-5795634C5BD5" aria-selected="false" id="tab-56C7AC42-F7D6-4AD4-A13F-5795634C5BD5" > Protein Identification <div style="font-weight: 700; height: 0; overflow: hidden;" aria-hidden="true"> Protein Identification</div> </button> </li> <li style="order:5" > <button tabindex="-1" role="tab" class="tab__button" aria-controls="panel-F7F2AEC5-A579-4EB0-8B96-DAC883C52955" aria-selected="false" id="tab-F7F2AEC5-A579-4EB0-8B96-DAC883C52955" > Targeted Proteomics <div style="font-weight: 700; height: 0; overflow: hidden;" aria-hidden="true"> Targeted Proteomics</div> </button> </li> <li style="order:7" > <button tabindex="-1" role="tab" class="tab__button" aria-controls="panel-CA797D61-7D71-436D-AC05-F90D939485F8" aria-selected="false" id="tab-CA797D61-7D71-436D-AC05-F90D939485F8" > Flux Proteomics <div style="font-weight: 700; height: 0; overflow: hidden;" aria-hidden="true"> Flux Proteomics</div> </button> </li> </ul> <div class="tabs-container"> <div tabindex="0" role="tabpanel" class="tab" id="panel-0F029030-1B88-438D-92FE-611BBEF2116F" aria-labelledby="tab-0F029030-1B88-438D-92FE-611BBEF2116F" aria-hidden="false" style="order:2" > <div class="field-content"><p>Label free relative quantitation of protein abundances offers a simple and accurate means to compare the abundance of thousands of proteins between samples. This affordable methodology is suitable for both large and small sample numbers and serves to both identify the proteins present in a sample and to measure their relative abundance. Proteins present in each sample are subjected to tryptic digestion followed by individual injection onto our state-of-the-art Orbitap instruments.</p> <p>We offer two gradient lengths with more complex samples such as whole tissue lysates being ideally suited to longer analysis to allow greater depth of coverage of proteins present and a shorter gradient for less complex samples such as immunoprecipitations and subcellular fractions.</p> <p>This service includes:</p> <ul> <li>Sample digestion</li> <li>Cleanup</li> <li>Mass spec analysis</li> <li>Followed by data processing to supply a spreadsheet of proteins present</li> <li>Their normalised relative abundances across samples</li> </ul> <p>This service can be extended to include a detailed statistical analysis of the data generated along with data visualisation.</p></div> </div> <div tabindex="0" role="tabpanel" class="tab" id="panel-56C7AC42-F7D6-4AD4-A13F-5795634C5BD5" aria-labelledby="tab-56C7AC42-F7D6-4AD4-A13F-5795634C5BD5" aria-hidden="false" style="order:4" > <div class="field-content"><p>Our protein identification service offers a cut price entry to the world of proteomics and is suitable for routine protein identification and sample quality control tasks. Analysed on a short gradient on one of our Orbitrap instruments, this service can provide an extensive list of the protein(s) present in a sample and make estimates as to the relative proportion each protein constitutes of the total sample.</p></div> </div> <div tabindex="0" role="tabpanel" class="tab" id="panel-F7F2AEC5-A579-4EB0-8B96-DAC883C52955" aria-labelledby="tab-F7F2AEC5-A579-4EB0-8B96-DAC883C52955" aria-hidden="false" style="order:6" > <div class="field-content"><p>Our targeted proteomics service allows researchers to track the abundance of a smaller number of specific proteins across multiple samples. This analysis has the advantage of allowing the highest confidence measurements of relative abundance available in proteomics as well as the ability to show that the specified proteins are absent (below the limit of detection of the instrumentation). This technique is commonly described as a mass Western but is much faster, more quantitative, not prone to cross reactivity and can generally be tailored to specific isoforms of proteins.</p> <p>This service includes:</p> <ul> <li>Sample digestion</li> <li>Cleanup</li> <li>Mass spec analysis </li> <li>Followed by data processing to supply a spreadsheet of proteins present</li> <li>Their normalised relative abundances across samples</li> </ul></div> </div> <div tabindex="0" role="tabpanel" class="tab" id="panel-CA797D61-7D71-436D-AC05-F90D939485F8" aria-labelledby="tab-CA797D61-7D71-436D-AC05-F90D939485F8" aria-hidden="false" style="order:8" > <div class="field-content"><p>Protein flux analysis offers the opportunity to understand how much of which proteins are being translated and degraded in your experimental system. These measurements allow a much more detailed understanding of the dynamics of protein biology than simple abundance measurements. In this methodology a pulse of stable isotope labelled substrate is fed into the experimental system which is metabolically incorporated into the amino acid pool and subsequently into newly synthesised protein. By tracking the rate of accumulation of protein with incorporated stable isotopes relative to the pre-existing unlabelled protein, both the rate of translation and degradation can be derived from a set of samples.</p></div> </div> </div> </div> </div> </div> </div> </div> <div class="component container grid-container small-12 cell" > <div class="component-content grid-x grid-padding-x"> <div class="component text-liftout text-liftout--column-single small-12 cell bkg-clr--grey full-width"> <div class="component-content"> <div class="text-liftout__column"> <div class="text-liftout__content"> <h2 class="text-liftout__heading"> <span class="text-liftout__heading--font-01"></span> <span class="text-liftout__heading--font-02">Interested in our services?</span> </h2> <div class="text-liftout__synopsis"><p>Please submit an online sample information form and one of our team members will get back to you.</p></div> <div class="btn text-liftout__btn"> <a href="https://forms.office.com/Pages/ResponsePage.aspx?id=8EqJBSjL2EaHFnTNtG4iJldKJNXvhRRJp4gA7BgfgSNUNDlLV1FJQUFaUlJBVE4yNk5NNkZYTlU0VS4u" rel="noopener noreferrer" class="" target="_self">Submit sample</a> </div> </div> </div> </div> </div> </div> </div> <div class="component container grid-container small-12 cell component-margin-bottom--small" > <div class="component-content grid-x grid-padding-x"> <div class="component image-liftout image-liftout--overlap small-12 cell component-margin-bottom--none image-liftout--alt"> <div class="component-content"> <div class="image-liftout-container"><figure class="image-liftout__column image-liftout__column--left"><div class="image-liftout__image-container"><div class="image-liftout__image-asset field-promoicon"><img src="/cmca/-/media/project/uwa/uwa/cmca/proteomics---team_cropped2.png?h=536&iar=0&w=800&hash=B50C0197906006AC4E379AB91F3B0105" alt="Proteomics Team" width="800" height="536" loading="lazy" data-variantitemid="{7217CF43-9337-4837-9958-0C10BD24DCAB}" data-variantfieldname="PromoIcon" /></div></div></figure><div class="image-liftout__column image-liftout__column--right"><div class="image-liftout__content-container grid-x"><h2 class="image-liftout__content-heading">Our team</h2><div class="image-liftout__content-asset field-promotext2"><p>With several decades of dedicated proteomics research combined, our scientists can help you get the most out of your experiment.</p> <p>The WA proteomics team at UWA:</p> <ul> <li style="margin-top: 0.5rem;">Ritu Mehta, Research Assistant</li> <li><a rel="noopener noreferrer" href="https://research-repository.uwa.edu.au/en/persons/elke-stroeher" target="_blank">Dr Elke Stroeher</a>,聽our Facility Manager</li> <li><a rel="noopener noreferrer" href="https://research-repository.uwa.edu.au/en/persons/owen-duncan" target="_blank" style="outline-width: 0px;">Dr Owen Duncan</a>, our Scientific Lead</li> <li><a href="https://research-repository.uwa.edu.au/en/persons/harvey-millar">Professor Harvey Millar</a>, our Platform Lead</li> </ul></div></div></div></div> </div> </div> <div class="component image-liftout image-liftout--overlap small-12 cell component-margin-bottom--none"> <div class="component-content"> <div class="image-liftout-container"><figure class="image-liftout__column image-liftout__column--left"><div class="image-liftout__image-container"><div class="image-liftout__image-asset field-promoicon"><img src="/cmca/-/media/project/uwa/uwa/cmca/proteomics---instruments_800x534.png?h=534&iar=0&w=800&hash=9FFD914954C90E1A4DCB554920D15341" alt="Proteomics staff member using Proteomics equipment" width="800" height="534" loading="lazy" data-variantitemid="{BBDEC66E-025B-46FE-BD30-9FA6D94AA302}" data-variantfieldname="PromoIcon" /></div></div></figure><div class="image-liftout__column image-liftout__column--right"><div class="image-liftout__content-container grid-x"><h2 class="image-liftout__content-heading">Our instruments</h2><div class="image-liftout__content-asset field-promotext2"><p>The Proteomics Facility house some of the most powerful instruments available for delivering quantitative proteomics and flux proteomics services.</p> <ul> <li>Thermo Fisher Orbitrap Fusion</li> <li>Exploris 480</li> <li>Triple quadrupole instruments</li> </ul> <p>If you want to understand more about proteomics and how it can relate to your biological questions, contact <a href="/cdn-cgi/l/email-protection#7f0f0d100b1a1012161c0c3f0a081e511a1b0a511e0a"><span class="__cf_email__" data-cfemail="364644594253595b5f55457643415718535243185743">[email protected]</span></a>.</p></div></div></div></div> </div> </div> </div> </div> <div class="component container grid-container small-12 cell" > <div class="component-content grid-x grid-padding-x"> <div class="component component-heading component-heading--section small-12 cell container-padding--bottom-small"> <div class="component-content"> <h2 class="section-title field-title">FAQ</h2> </div> </div> <div class="component accordion small-12 cell" data-properties='{"expandOnHover":false,"expandedByDefault":false,"speed":500,"easing":"swing","canOpenMultiple":true,"canToggle":true,"isControlEditable":false}'> <div class="component-content"> <div> <ul class="items"> <li class="item"> <a href="#" class="toggle-header"> <div class="label"> How much sample do I need to submit? </div> </a> <div class="toggle-content"> <div class="field-content"><p>Our sample requirements vary depending on what is needed from the analysis and how expensive it is to generate. 1x10^6 for most mammalian cell types is a good starting point but we can generate good data with tens of thousands of cells if that's what is available. For other sample types, we typically ask for 100ug of protein for complex (contains many different proteins) samples such as cell lysates or a few micrograms for simple samples such as immuno-precipitations.</p></div> </div> </li> <li class="item"> <a href="#" class="toggle-header"> <div class="label"> How should I prepare my samples? </div> </a> <div class="toggle-content"> <div class="field-content"><p>We typically like to receive cell pellets, supernatant removed, then snap frozen and dropped off to the Bayliss building foyer at a time arranged with staff. Where this is not applicable, please contact us so we can give specific advice.</p></div> </div> </li> <li class="item"> <a href="#" class="toggle-header"> <div class="label"> How much does your service cost? </div> </a> <div class="toggle-content"> <div class="field-content"><p>A few examples of pricing for non-commercial research, label free quantitation runs would be:</p> <ul> <li>6 samples ~$1000</li> <li>24 samples ~$3000</li> </ul></div> </div> </li> <li class="item"> <a href="#" class="toggle-header"> <div class="label"> What does your service include? </div> </a> <div class="toggle-content"> <div class="field-content"><p>The standard label free quant service includes protein extraction, quantitation, digestion and sample prep then run on a 90 min LC gradient on one of our orbitraps. Results are provided as a spreadsheet with rows being proteins identified and columns being the samples provided. We can also analyse and provide differential expression results though a bioinformatics fee may be invoked if it's an involved analysis.</p></div> </div> </li> <li class="item"> <a href="#" class="toggle-header"> <div class="label"> Can I ask other questions? </div> </a> <div class="toggle-content"> <div class="field-content">Of course! email <a href="/cdn-cgi/l/email-protection#a5d5d7cad1c0cac8ccc6d6e5d0d2c48bc0c1d08bc4d0"><span class="__cf_email__" data-cfemail="1e6e6c716a7b7173777d6d5e6b697f307b7a6b307f6b">[email protected]</span></a> and we will endeavour to answer swiftly.</div> </div> </li> </ul> </div> </div> </div> <div class="component component-heading component-heading--section small-12 cell container-padding--bottom-small"> <div class="component-content"> <h2 class="section-title field-title">Location and contact</h2> </div> </div> <div class="component rich-text small-12 medium-6 cell"> <div class="component-content"> <strong>Email:</strong><br /> <a href="/cdn-cgi/l/email-protection#93e3e1fce7f6fcfefaf0e0d3e6e4f2bdf6f7e6bdf2e6b3"><span class="__cf_email__" data-cfemail="c3b3b1acb7a6acaeaaa0b083b6b4a2eda6a7b6eda2b6">[email protected]</span></a><br /> <br /> <strong>Address:</strong><br /> The University of Western Australia<br /> School of Molecular Sciences<br /> Room G28, Bayliss Building,<br /> Fairway Entry, Crawley WA 6009<br /> <div> </div> </div> </div> <div class="component plain-html small-12 medium-6 cell"> <div class="component-content"> <iframe width="600" height="420" frameBorder="0" scrolling="no" marginHeight="0" marginWidth="0"src="https://use.mazemap.com/embed.html#v=1&config=uwa&zlevel=1&center=115.818316,-31.981948&zoom=18&sharepoitype=poi&sharepoi=1000462838&campusid=309&utm_medium=iframe" style={{ border: '1px solid grey' }} allow="geolocation"></iframe> </div> </div> </div> </div> </div> </main>   <footer> <div id="footer" class="grid-container"> <div class="component container grid-container initial-12 cell sxa-footer" > <div class="component-content grid-x grid-padding-x"> <div class="component container grid-container cell" > <div class="component-content grid-x grid-padding-x"> <div class="component container grid-container initial-12 large-6 cell remove-padding-left-right" > <div class="component-content grid-x grid-padding-x"> <div class="component container grid-container initial-12 medium-6 cell remove-padding-left-right" > <div class="component-content grid-x grid-padding-x"> <div class="component rich-text initial-12 medium-12 cell footer-mb"> <div class="component-content"> <p class="bold upper"><strong>THE UNIVERSITY OF WESTERN AUSTRALIA</strong></p> <br /> <p>35 Stirling Highway<br /> Perth WA 6009 Australia</p> <p class="ft-number"><a href="tel:+61864886000">+61 8 6488 6000</a></p> <p class="ft-number--emergency">Emergency<br /> <a href="tel:+61864882222"><span>+61 8 6488 2222</span></a></p> <p>CRICOS 00126G | PRV12169, Australian University</p> </div> </div> </div> </div> <div class="component container grid-container initial-12 medium-6 cell remove-padding-left-right" > <div class="component-content grid-x grid-padding-x"> <div class="component link-list footer-mb footer-list initial-8 medium-10 cell footer-mb-override"> <div class="component-content"> <ul> <li class="item0 odd first"> <div class="field-link"><a data-variantfieldname="Link" target="|Custom" href="https://www.uwa.edu.au/accessibility" data-variantitemid="{1D2958E7-EBE6-4AB7-B2FE-1E94FB98D91C}">Accessibility</a></div> </li> <li class="item1 even"> <div class="field-link"><a data-variantfieldname="Link" href="https://www.web.uwa.edu.au/askuwa" data-variantitemid="{C60053B6-DE33-4454-83BE-FEEB525A76E8}">Ask UWA</a></div> </li> <li class="item2 odd"> <div class="field-link"><a data-variantfieldname="Link" href="https://www.jobs.uwa.edu.au/" data-variantitemid="{8847231C-C354-4278-9801-73720E334A67}">Jobs</a></div> </li> <li class="item3 even"> <div class="field-link"><a data-variantfieldname="Link" target="|Custom" href="https://www.uwa.edu.au/privacy" data-variantitemid="{6F659BEC-397F-4C6E-A7C9-176912198C61}">Privacy</a></div> </li> <li class="item4 odd"> <div class="field-link"><a data-variantfieldname="Link" href="https://uniwa.sharepoint.com/sites/staffintranet" data-variantitemid="{58932937-5256-4147-B334-6C40E8C38334}">Staff portal</a></div> </li> <li class="item5 even last"> <div class="field-link"><a data-variantfieldname="Link" target="|Custom" href="https://www.uwa.edu.au/disclaimer-copyright" data-variantitemid="{9D63773A-E2D1-4BFC-9C50-38E3E3410817}">Terms of use</a></div> </li> </ul> </div> </div> <div class="component plain-html cell footer-language"> <div class="component-content"> <div class="footer-language-button" tabindex="0"> <span class="footer-language-button--australia"><em class="flag"></em>Australia<em class="fa fa-chevron-down"></em></span> <a class="footer-language-button--china" href="http://www.universityofwesternaustralia.cn/"><em class="flag"></em>Chinese</a> </div> </div> </div> </div> </div> </div> </div> <div class="component container grid-container initial-12 large-6 cell flex-vertical-space-between footer-acknowledgement footer-mb-override" > <div class="component-content grid-x grid-padding-x"> <div class="component rich-text initial-12 small-8 large-10 cell remove-padding-left-right footer-mb overlay-footer-indigenous"> <div class="component-content"> <p class="ft-disclaimer">The University of Western Australia acknowledges that its campus is situated on Noongar land,  and that Noongar people remain the spiritual and cultural custodians of their land,  and continue to practise their values, languages, beliefs and knowledge.</p> <p class="ft-text"><a href="https://www.indigenous.uwa.edu.au/">Indigenous Commitment</a></p> </div> </div> </div> </div> </div> </div> <div class="component container grid-container initial-12 large-12 cell footer-top-bottom-line" > <div class="component-content grid-x grid-padding-x"> <div class="component link-list js-link-list initial-12 small-6 cell ft-link-list-center"> <div class="component-content"> <ul> <li class="item0 odd first"> <div class="field-link"><a class="ft-icon fb-icon" data-variantfieldname="Link" target="_blank" title="Follow us on Facebook" rel="noopener noreferrer" href="https://www.facebook.com/universitywa" data-variantitemid="{CEC64A93-C2D1-417E-86BC-3ADA8627B4AE}"> </a></div> </li> <li class="item1 even"> <div class="field-link"><a class="ft-icon instagram-icon" data-variantfieldname="Link" target="_blank" title="Follow us on Instagram" rel="noopener noreferrer" href="https://www.instagram.com/universitywa" data-variantitemid="{78CFA127-4941-4BAC-AB10-A57DCD09AA32}"> </a></div> </li> <li class="item2 odd"> <div class="field-link"><a class="ft-icon twitter-icon" data-variantfieldname="Link" target="_blank" title="Follow us on Twitter" rel="noopener noreferrer" href="https://twitter.com/uwanews" data-variantitemid="{8B60AFFC-EA4D-41AE-94A1-187DF6D28518}"> </a></div> </li> <li class="item3 even"> <div class="field-link"><a class="ft-icon youtube-icon" data-variantfieldname="Link" target="_blank" title="Follow us on Youtube" rel="noopener noreferrer" href="https://www.youtube.com/user/universitywa" data-variantitemid="{EEB4CA5E-F9F4-4772-9AEA-97B06B84C9B4}"> </a></div> </li> <li class="item4 odd last"> <div class="field-link"><a class="ft-icon linkedin-icon" data-variantfieldname="Link" target="_blank" title="Follow us on LinkedIn" rel="noopener noreferrer" href="https://www.linkedin.com/school/university-of-western-australia/" data-variantitemid="{ECDFA194-602D-4B85-B5AA-18736C943844}"> </a></div> </li> </ul> </div> </div> <div class="component plain-html initial-12 large-6 cell grid-container"> <div class="component-content"> <div class="footer-membership-list"> <a aria-label="Go8 logo" rel="noopener noreferrer" class="ft-icon-md go8-icon" href="https://go8.edu.au/" target="_blank"> </a> </div> </div> </div> </div> </div> <div class="component content cell"> <div class="component-content"> <span><Button class="js-this-page-btn">This page</button></span><div class="js-this-page-info"><div><strong>Updated:</strong>15/11/2024 09:51 AM<span> (this date excludes nested assets)</span></div><div><strong>Content ID:</strong><span>{05177626-788E-476E-9FD9-5E2FB7F6FB68}</span></div></div> </div> </div> </div> </div><div class="component plain-html cell" id="sxa-footer-back-to-top"> <div class="component-content"> <a href="#wrapper">Back to top</a> </div> </div> <div class="component-badges badges" style="display:none;"> <div class="tooltip-content" id="badge-careers-employability" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/careers--employability-badge.svg"><div class="tooltip-title field-badgetitle">Careers Employability</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Careers Employability badge.</div></div><div class="tooltip-content" id="badge-community-impact" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/community-impact-badge.svg"><div class="tooltip-title field-badgetitle">Community Impact</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Community Impact badge.</div></div><div class="tooltip-content" id="badge-digital-capability" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/digital-capability-badge.svg"><div class="tooltip-title field-badgetitle">Digital Knowledge</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Digital Knowledge badge.</div></div><div class="tooltip-content" id="badge-entrepreneurialism-innovation" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/entrepreneurialism--innovation-badge.svg"><div class="tooltip-title field-badgetitle">Entrepreneurialism and Innovation</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Entrepreneurialism and Innovation badge.</div></div><div class="tooltip-content" id="badge-global-citizen" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/global-citizen-badge.svg"><div class="tooltip-title field-badgetitle">Global Citizen</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Global Citizen badge.</div></div><div class="tooltip-content" id="badge-indigenous-knowledge" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/indigenous-awareness-badge.svg"><div class="tooltip-title field-badgetitle">Indigenous Knowledge</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Indigenous Knowledge badge.</div></div><div class="tooltip-content" id="badge-leadership" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/leadership-badge.svg"><div class="tooltip-title field-badgetitle">Indigenous Knowledge</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Leadership badge.</div></div><div class="tooltip-content" id="badge-professional-development" data-icon="/cmca/-/media/project/uwa/uwa/global/badge-icons/professional-development-badge.svg"><div class="tooltip-title field-badgetitle">Professional Development</div><div class="tooltip-description field-badgedescription">The real-world skills learned in this activity contribute towards a Professional Development badge.</div></div> </div> </div> </footer>  </div> <div class="privacy-warning permisive" style=""> <div class='close'> <a onclick="XA.cookies.removeCookieWarning();">X</a> </div> <div class="info"> Cookies help us improve your website experience. By using our website, you agree to our use of cookies. </div> <div class="submit"> <a onclick="XA.cookies.createCookie('privacy-notification', 1, 365);XA.cookies.removeCookieWarning();">Confirm</a> </div> </div> <script data-cfasync="false" src="/cdn-cgi/scripts/5c5dd728/cloudflare-static/email-decode.min.js"></script><script src="/research/-/media/base-themes/core-libraries/scripts/optimized-min.js?t=20230527T093311Z"></script><script src="/research/-/media/base-themes/xa-api/scripts/optimized-min.js?t=20230527T093315Z"></script><script src="/research/-/media/base-themes/main-theme/scripts/optimized-min.js?t=20230527T093318Z"></script><script src="/research/-/media/base-themes/google-maps-js-connector/scripts/optimized-min.js?t=20230527T093322Z"></script><script src="/research/-/media/base-themes/maps/scripts/optimized-min.js?t=20230527T093324Z"></script><script src="/research/-/media/base-themes/resolve-conflicts/scripts/optimized-min.js?t=20230527T093326Z"></script> <script defer src="/Theme-SXA/dist/masterbrand/main.js?v=638643367870000000"></script> <script defer src="https://static.weboffice.uwa.edu.au/visualid/devcore/sitecore/js/navigation.js"></script>  </body> </html>

CINXE.COM

WA Proteomics Facility | CMCA | UWA