Synthesis and Cytotoxic Activity of New Quinazolinone-Based Compounds against Human Breast Cancer Cell Line MCF-7

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of New Quinazolinone-Based Compounds against Human Breast Cancer Cell Line MCF-7</title> <meta name="description" content="Synthesis and Cytotoxic Activity of New Quinazolinone-Based Compounds against Human Breast Cancer Cell Line MCF-7"> <meta name="keywords" content="Quinazoline Schiff base, apoptosis, MCF-7 human breast cancer cell line, caspase, NF-魏B translocation"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a 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87352</div> </div> </div> </div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Synthesis and Cytotoxic Activity of New Quinazolinone-Based Compounds against Human Breast Cancer Cell Line MCF-7</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Zahedifard">Maryam Zahedifard</a>, <a href="https://publications.waset.org/abstracts/search?q=Fadhil%20Lafta%20Faraj"> Fadhil Lafta Faraj</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Hajrezaie"> Maryam Hajrezaie</a>, <a href="https://publications.waset.org/abstracts/search?q=Nazia%20Abdul%20Majid"> Nazia Abdul Majid</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmood%20Ameen%20Abdulla"> Mahmood Ameen Abdulla</a>, <a href="https://publications.waset.org/abstracts/search?q=Hapipah%20Mohd%20Ali"> Hapipah Mohd Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the current study, we prepared two new quinazoline schiff bases through condensation reaction of 2-aminobenzhydrazide with 5-bromosalicylaldehyde and 3-methoxy-5-bromosalicylaldehyde. The chemical structures of both newly synthesized compounds (1 and 2) were confirmed by FT-IR and X-ray crystallography studies. The cytotoxic effect of compounds was investigated against MCF-7 human breast cancer cells. MTT results showed that (1) and (2) decreased the viability of MCF-7 cells in a time-dependent manner, exhibiting an IC50 value of 3.23 卤 0.28 碌g/mL and 3.41 卤 0.34 碌g/mL, respectively, after a 72-hours treatment period. In contrast, they did not show significant anti-proliferative effect towards MCF-10A normal breast cells and WRL-68 normal liver cells. We found a perturbation in mitochondrial membrane potential and increased cytochrome c release from the mitochondria to the cytosol, suggesting an activation of apoptosis by compounds, which was confirmed by activation of the initiator caspase-9 and the executioner caspases-3/7. (1) was also able to trigger extrinsic pathway via activation of caspase-8 and inhibition of NF-魏B translocation. The acute toxicity test showed no toxicity effect of the compounds in rats. Our results showed that the selected synthesized compounds are highly potent to induce apoptosis in MCF-7 cells via either intrinsic or extrinsic mitochondrial pathway. <iframe src="https://publications.waset.org/abstracts/13587.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Quinazoline%20Schiff%20base" title="Quinazoline Schiff base">Quinazoline Schiff base</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a>, <a href="https://publications.waset.org/abstracts/search?q=MCF-7%20human%20breast%20cancer%20cell%20line" title=" MCF-7 human breast cancer cell line"> MCF-7 human breast cancer cell line</a>, <a href="https://publications.waset.org/abstracts/search?q=caspase" title=" caspase"> caspase</a>, <a href="https://publications.waset.org/abstracts/search?q=NF-%CE%BAB%20translocation" title=" NF-魏B translocation"> NF-魏B translocation</a> </p> <a href="https://publications.waset.org/abstracts/13587/synthesis-and-cytotoxic-activity-of-new-quinazolinone-based-compounds-against-human-breast-cancer-cell-line-mcf-7" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13587.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">491</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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Synthesis and Cytotoxic Activity of New Quinazolinone-Based Compounds against Human Breast Cancer Cell Line MCF-7