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li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:980px!important;margin:0}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{margin:20px 0;padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-transparent #Top_bar .menu > li > a{margin:5px 0}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-top.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-bottom.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span:not(.description){border-right-width:0}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank{padding-left:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:none;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{display:none}#Header_creative #Top_bar .top_bar_right_wrapper{top:0}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0!important}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto;bottom:0}#Header_creative #Top_bar a#header_cart{display:inline-block;float:none;top:3px}#Header_creative #Top_bar a#search_button{display:inline-block;float:none;top:3px}#Header_creative #Top_bar .wpml-languages{display:inline-block;float:none;top:0}#Header_creative #Top_bar .wpml-languages.enabled:hover a.active{padding-bottom:9px}#Header_creative #Top_bar a.button.action_button{display:inline-block;float:none;top:16px;margin:0}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl.nice-scroll #Header_creative{margin-right:10px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul li.hover a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1130px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;filter:alpha(opacity = 97);-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{max-width:1130px;left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}.layout-boxed.header-boxed.nice-scroll #Top_bar.is-sticky{margin-left:-5px}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none}#Top_bar.is-sticky .top_bar_right{top:-4px;height:auto}#Top_bar.is-sticky .top_bar_right_wrapper{top:15px}.header-plain #Top_bar.is-sticky .top_bar_right_wrapper{top:0}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right a{color:rgba(255,255,255,.5)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.3);border-color:rgba(0,0,0,0.1)}}@media only screen and (min-width:768px) and (max-width:1130px){.header_placeholder{height:0!important}}@media only screen and (max-width:1129px){#Top_bar #menu{display:none;height:auto;width:100%;bottom:auto;top:100%;right:1px;position:absolute;margin:0}#Top_bar a.responsive-menu-toggle{display:block}#Top_bar #menu > ul{width:100%;float:left}#Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}#Top_bar #menu ul li a{padding:0 25px;margin:0;display:block;height:auto;line-height:normal;border:none}#Top_bar #menu ul li a:after{display:none}#Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}#Top_bar #menu ul li a span.description{margin:0 0 0 5px}#Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:15px;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Top_bar #menu ul li.hover > 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ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/high-throughput-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">High-throughput Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26914 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26915" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26915 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220-plus-focused-ultrasonicator-500569"><span class="ubermenu-target-title ubermenu-target-text">LE220-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High-throughput, 1 to 96 tube capacity</span></a></li><li id="menu-item-26916" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26916 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220r-plus-focused-ultrasonicator-500578"><span class="ubermenu-target-title ubermenu-target-text">LE220R-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Robotic version of the LE220-plus</span></a></li><li id="menu-item-30065" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30065 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/le220rsc-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">LE220Rsc</span></a></li></ul></li><li id="menu-item-26917" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26917 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/on-deck-automation-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">On-Deck Automation Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26917 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26918" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26918 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/r230-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">R230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">On-deck, next generation Focused-ultrasonicator</span></a></li></ul></li><li id="menu-item-26919" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26919 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/cryoprep"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP<sup>®</sup> Extraction Systems</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26919 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26920" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26920 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp01-cryoprep-manual-dry-pulverizer-500230"><span class="ubermenu-target-title ubermenu-target-text">CP01 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 15 mg to 2 g</span></a></li><li id="menu-item-26921" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26921 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001"><span class="ubermenu-target-title ubermenu-target-text">CP02 Automated Dry Pulverizer</span></a></li><li id="menu-item-26922" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26922 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp03-cryoprep-manual-dry-pulverizer-500049"><span class="ubermenu-target-title ubermenu-target-text">CP03 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 2 to 5 g</span></a></li></ul></li><li id="menu-item-26923" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26923 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/sonolab-software"><span class="ubermenu-target-title ubermenu-target-text">SonoLab Software</span></a></li></ul></li><li id="menu-item-26927" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26927 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents"><span class="ubermenu-target-title ubermenu-target-text">Kits & Reagents</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26927 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-41083" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-41083 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truprep-protein/"><span class="ubermenu-target-title ubermenu-target-text">truPREP® Protein Kits</span></a></li><li id="menu-item-35108" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-35108 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truxtrac-ffpe-na-auto-96-kits"><span class="ubermenu-target-title ubermenu-target-text">truXTRAC® FFPE SMART Solutions</span></a></li><li id="menu-item-26931" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26931 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/cryoprep-0"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Starter Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cryoPREP Sample Tubes & Holders</span></a></li><li id="menu-item-26933" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26933 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/nucleic-acids-shearing-kits-and-buffers"><span class="ubermenu-target-title ubermenu-target-text">Nucleic Acids Shearing Kits and Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">truSHEAR, truPOP, DNA Verification Kits</span></a></li><li id="menu-item-26929" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26929 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-ffpe-kits"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> FFPE Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">FFPE Biomolecule Extraction Kits</span></a></li><li id="menu-item-26934" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26934 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-other"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cfDNA, DBS, MALDI-TOF</span></a></li><li id="menu-item-26935" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26935 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/protein-extraction-buffers"><span class="ubermenu-target-title ubermenu-target-text">Protein Extraction Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Native, Total Protein, & Detergent-free Buffers</span></a></li><li id="menu-item-26930" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26930 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truchip"><span class="ubermenu-target-title ubermenu-target-text">truChIP<sup>®</sup></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Chromatin Shearing Kits</span></a></li></ul></li><li id="menu-item-26936" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26936 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables"><span class="ubermenu-target-title ubermenu-target-text">Consumables – Tubes and Vials</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26936 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26938" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26938 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/afa-tube"><span class="ubermenu-target-title ubermenu-target-text">AFA-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Laser etched polymer vessel for high-throughput workflows</span></a></li><li id="menu-item-26939" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26939 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/microtube"><span class="ubermenu-target-title ubermenu-target-text">microTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Small sample volumes, 130 µl or less</span></a></li><li id="menu-item-26940" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26940 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/millitube"><span class="ubermenu-target-title ubermenu-target-text">milliTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">1 ml or 2 ml sample volume. Good for chromatin shearing</span></a></li><li id="menu-item-26942" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26942 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/other-afa-tubes"><span class="ubermenu-target-title ubermenu-target-text">Other AFA-TUBEs</span></a></li><li id="menu-item-26943" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26943 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/afa-process-vessels/"><span class="ubermenu-target-title ubermenu-target-text">AFA Process Vessels</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, low temperature processing</span></a></li><li id="menu-item-26944" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26944 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/g-tube"><span class="ubermenu-target-title ubermenu-target-text">g-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">DNA shearing fragments 6 to 20 kb</span></a></li></ul></li><li id="menu-item-26945" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26945 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Consumables</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26945 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26947" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26947 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables/tissuetube"><span class="ubermenu-target-title ubermenu-target-text">tissueTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Tissue processing devices</span></a></li></ul></li><li id="menu-item-26949" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26949 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories"><span class="ubermenu-target-title ubermenu-target-text">Accessories</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26949 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26952" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26952 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/automation-accessories"><span class="ubermenu-target-title ubermenu-target-text">Automation Accessories</span></a></li><li id="menu-item-41270" class="ubermenu-item ubermenu-item-type-post_type ubermenu-item-object-page ubermenu-item-41270 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/r230-accessories"><span class="ubermenu-target-title ubermenu-target-text">R230 Accessories</span></a></li><li id="menu-item-26953" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26953 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/m220-accessories"><span class="ubermenu-target-title ubermenu-target-text">M220 Accessories</span></a></li><li id="menu-item-26954" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26954 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/me220-accessories"><span class="ubermenu-target-title ubermenu-target-text">ME220 Accessories</span></a></li><li id="menu-item-30718" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30718 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/ml230-accessories"><span class="ubermenu-target-title ubermenu-target-text">ML230 Accessories</span></a></li><li id="menu-item-26955" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26955 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/s220-accessories"><span class="ubermenu-target-title ubermenu-target-text">S220 Accessories</span></a></li><li id="menu-item-26956" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26956 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-evolution-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i> Accessories</span></a></li><li id="menu-item-26957" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26957 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220 Accessories</span></a></li><li id="menu-item-26958" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26958 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/l-series-accessories"><span class="ubermenu-target-title ubermenu-target-text">L-Series Accessories</span></a></li><li id="menu-item-26959" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26959 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp01-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP01 Accessories</span></a></li><li id="menu-item-26960" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26960 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp02-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP02 Accessories</span></a></li><li id="menu-item-26961" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26961 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp03-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP03 Accessories</span></a></li><li id="menu-item-26963" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26963 ubermenu-item-auto 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!important;}.vc_custom_1714642258004{margin-bottom: 0px !important;}.vc_custom_1633618807684{margin-top: 13px !important;margin-bottom: 0px !important;}.vc_custom_1583492989135{padding-right: 20px !important;padding-left: 20px !important;}.vc_custom_1714639431512{margin-bottom: 8px !important;padding-bottom: 8px !important;}.vc_custom_1587016276581{margin-bottom: 50px !important;}.vc_custom_1618246760901{margin-bottom: 0px !important;}</style> <div id="Content" class="cms_mage_page" style="padding: 0px;"> <div class="content_wrapper clearfix"> <div class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid shearing_banner vc_custom_1583475962885 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner vc_custom_1583475591897"><div class="wpb_wrapper"><h1 style="color: #ffffff;text-align: center" class="vc_custom_heading vc_do_custom_heading vc_custom_1646289722418" >Sample Preparation for Proteomics Workflows</h1><div style="color: #ffffff;text-align: center" class="vc_custom_heading vc_do_custom_heading h3" >Covaris Adaptive Focused Acoustics® (AFA®) technology provides the most accurate and reproducible sample preparation solution for proteins, enabling non-contact, highly efficient extraction and processing for any downstream analysis method.</div></div></div></div></div><div class="vc_row-full-width vc_clearfix"></div><div class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1583489874470 vc_column-gap-20"><div class="common-pages-left-container PrdMenu clearfix wpb_column vc_column_container vc_col-sm-3 vc_col-has-fill"><div class="vc_column-inner vc_custom_1583479740629"><div class="wpb_wrapper"><h4 style="color: #ffffff;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h4 vc_custom_1719423879989" >Contents</h4> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <ul id="shearing-sidebar"> <li class="active"><a style="cursor: pointer; display: block; text-decoration: none;" href="#featured_article">Featured Article</a></li> <li><a style="cursor: pointer; display: block; text-decoration: none;" href="#overview">Overview</a></li> <li><a class="sidebar_anchor flex_list proteinExtraction" style="cursor: pointer; display: flex !important; text-decoration: none;" href="#protein">Protein Extraction and Processing For Mass Spectrometry<br /> </a></p> <ul id="ul_sidebar" class="sidebar_ul"> <li><a style="cursor: pointer; display: flex !important; text-decoration: none;" href="#fragment">Single Pot extraction using SP3 clean-up<br /> </a></li> <li><a style="cursor: pointer; display: flex !important; text-decoration: none;" href="#strap">Formalin Fixed Paraffin Embedded Scrolls using S-Trap<br /> </a></li> <li><a style="cursor: pointer; display: flex !important; text-decoration: none;" href="#trypsin">Enhanced trypsin digestion using AFA @ for improved recovery of membrane proteins<br /> </a></p> <ul class="sidebarUlChild" style="width: 100%;"> <li style="display: inline-block;"><a style="cursor: pointer; text-decoration: none; display: block; /* padding-left: 20px;" href="#proteinExtraction">Protein Extraction and Processing For SDS-PAGE and Antibody-based Assays<br /> </a></li> <li style="display: inline-block;"><a style="cursor: pointer; text-decoration: none; display: block;" href="#plants">Plants, Yeasts, Bacteria and other non-mamalian sample types<br /> </a></li> </ul> </li> </ul> </li> <li class=""><a style="cursor: pointer; display: block; text-decoration: none;" href="#featured_product">Featured Products</a></li> <li class=""><a style="cursor: pointer; display: grid; text-decoration: none;" href="#references">References</a></li> </ul> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1626784143318"><div class="wpb_wrapper"><section class="vc_cta3-container"><div class="vc_general vc_do_cta3 vc_cta3 h1 fw-bold vc_cta3-style-custom vc_cta3-shape-rounded vc_cta3-align-center vc_cta3-icon-size-md vc_cta3-actions-bottom" style="background-color:rgb(232,243,255);background-color:rgba(232,243,255,0.49);"><div class="vc_cta3_content-container"><div class="vc_cta3-content"><header class="vc_cta3-content-header"><h2 style="color:#0c0c0c;">Connect with an expert!</h2></header><p><a href="https://www.covaris.com/contact-us/">Learn what Covaris can do for your proteomics workflows.</a></p> </div><div class="vc_cta3-actions"><div class="vc_btn3-container vc_btn3-center vc_do_btn" ><a onmouseleave="this.style.borderColor='#015cb8'; this.style.backgroundColor='transparent'; this.style.color='#015cb8'" onmouseenter="this.style.borderColor='rgba(1,92,184,0.2)'; this.style.backgroundColor='rgba(1,92,184,0.2)'; this.style.color='#015cb8';" style="border-color:#015cb8; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-outline-custom" href="https://www.covaris.com/contact-us/" title="SPEAK NOW" target="_blank">CONNECT NOW</a></div></div></div></div></section><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h2" >Featured Article</div><div class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1626778528063 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <p><img class="" src="https://www.covaris.com/wp/wp-content/uploads/Untitled-1.jpg" width="511" height="446" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading h6" >Fixed tissues (e.g. with Formalin or OCT) display a valuable depository of clinically relevant primary samples, whether they are embedded in paraffin (FFPE) or fresh. These samples provide great information to elucidate proteome changes during different scenarios such as treatment response, disease courses, remission etc.</div><div class="vc_btn3-container vc_btn3-inline vc_custom_1627330537754 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020153.pdf" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-right"></i></a></div></div></div></div></div><div id="overview" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1620314714633"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Reproducible, Hands-free Protein Sample Preparation with Covaris AFA®</h2> <div class="wpb_text_column wpb_content_element vc_custom_1714639645775 instrument_bullet_list" > <div class="wpb_wrapper"> <p class="custom_h5">Sample preparation can significantly impact the quality of data in both research and clinical fields. This is especially true in relation to proteomics sample preparation, as proteins are an extremely diverse community of macromolecules, present in an extraordinarily wide range of concentrations, and they cannot be amplified.</p> <p class="custom_h5">Many methods and protocols to isolate proteins are available, but none have the same capabilities as AFA for:</p> <ul> <li>Covering the diversity of samples (FFPE, LCM, fresh tissue, cells, bacteria, yeast...)</li> <li>Reducing human error</li> <li>Decreasing hands-on time</li> <li>Increasing speed and throughput</li> <li>Significantly improving reproducibility and reliability</li> </ul> </div> </div> </div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1_quote"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583730045887" > <div class="wpb_wrapper"> <p><img class="aligncenter" src="/wp/wp-content/uploads/2020/02/quote.png"" /></p> </div> </div> <div style="color: #2c2c2c;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" id="em"><em>“With the Covaris LE220Rsc we can now fully perform sample preparation for challenging samples, such as fresh frozen and FFPE tissue, in 96-well format. This dramatically improves our throughput to hundreds of thousands of samples per year for true large scale proteomics.”</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" ><em>Dr. Lukas Reiter</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" ><em>Chief Technology Officer - Biognosys</em></div></div></div></div></div><div id="testimonial" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1619803558328"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1714641614470 h6" > <div class="wpb_wrapper"> <p class="custom_h5">Covaris AFA protein sample preparation can accommodate every sample type for any downstream application in a variety of volumes and throughputs, while still leaving the choice of buffers and clean-up methods open like SP3 (single pot solid phase sample preparation, [1]). Many laboratories need automated solutions for protein sample preparation with better efficiency and higher quality results and Covaris <a href="/catalogsearch/result/?q=Focused-ultrasonicator">Focused-ultrasonicators</a> are proving to be an extremely robust and flexible solution with <a href="https://www.covaris.com/news/press-release-univ-copenhagen-proteomics/" target="_blank" rel="noopener noreferrer">growing adoption</a> in the proteomics space.</p> </div> </div> <div class="wpb_video_widget wpb_content_element vc_clearfix vc_custom_1583915206082 vc_video-aspect-ratio-169 vc_video-el-width-100 vc_video-align-center" > <div class="wpb_wrapper"> <div class="wpb_video_wrapper"><iframe title="Reproducible & Automated Sample Preparation for Clinical Proteomics with Focused-ultrasonication" width="500" height="281" src="https://www.youtube.com/embed/GM8wMZskLlo?feature=oembed" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" referrerpolicy="strict-origin-when-cross-origin" allowfullscreen></iframe></div> </div> </div> <div class="wpb_text_column wpb_content_element vc_custom_1591166618070 custom_h5" > <div class="wpb_wrapper"> <p style="text-align: center;">Watch the video testimonial from Dr. Fabian Coscia at the Novo Nordisk Foundation Center for Protein Research to learn how they developed a reproducible & automated sample preparation solution for clinical proteomics with Covaris Focused-ultrasonicator.</p> </div> </div> </div></div></div></div><div id="protein" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583734617284"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h2" >Protein Extraction and Processing For Mass Spectrometry</div> <div class="wpb_text_column wpb_content_element vc_custom_1714641643427 h6" > <div class="wpb_wrapper"> <p>Covaris has developed workflows and protocols with world leading laboratories looking for improved extraction, automated parallel processing and sample input decrease. Mass spectrometry is the downstream analytical method of choice, but the workflows could be applied to other applications such as western blotting, ELISA or multiplex assays.</p> </div> </div> </div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1_quote"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1626780586146" > <div class="wpb_wrapper"> <p><img class="aligncenter" src="/wp/wp-content/uploads/2020/02/quote.png"" /></p> </div> </div> <div style="color: #2c2c2c;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" ><em>“We are very impressed with our Covaris AFA systems. AFA has shown to be ideal for our facility as we deal with a very wide range of different sample types. This single technology has been successful at effectively disrupting and solubilising every sample we have given it. They are professionally designed, easy to use and highly controllable equipment and our LE220-plus is now one of the most essential and highly used systems in our facility.”</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Dr. David Knight</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Biological Mass Spectrometry Facility Manager, The University of Manchester</em></div></div></div></div></div><div id="fragment" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h2 vc_custom_1714644759279" >Single Pot extraction using SP3 clean-up</div> <div class="wpb_text_column wpb_content_element vc_custom_1729079375073 h6" > <div class="wpb_wrapper"> <p>In this workflow used on mammalian cells (1,000 to 400,000) and fresh frozen tissues below 10mg, samples are collected and transferred into a Covaris vessel where they are lysed in the desired buffer using AFA. Following lysis, alkylation and reduction reagents are added; AFA can be used again for efficient mixing and enhanced preparation for digestion. SP3 (Single Pot Solid Phase Sample Preparation) beads are added for the washing steps[1]. Ultimately, trypsin or any appropriate enzyme can be added for digestion.</p> <p>The exact protocol described above was developed in the publication <a href="http://bit.ly/3awz4kT" target="_blank" rel="noopener noreferrer">Automated sample preparation with SP3 for low‐input clinical proteomics</a> by Mueller et al. [2] on their <a href="https://www.covaris.com/le220r-plus-focused-ultrasonicator-500578"><u>LE220R-plus</u></a>. This protocol will work identically on <a href="https://www.covaris.com/le220rsc-focused-ultrasonicator">LE220Rsc</a> and <a href="https://www.covaris.com/le220-plus-focused-ultrasonicator-500569">LE220-plus</a> [3] for <a href="https://www.covaris.com/catalogsearch/result/?q=AFA-TUBE">AFA-TUBE strips and plates</a>. It has been adapted for other instruments using the same consumables like the <a href="https://www.covaris.com/r230-focused-ultrasonicator-500620">R230</a> or the <a href="https://www.covaris.com/ml230-focused-ultrasonicator-500656">ML230</a>. To learn more about developing this protocol, check our proteomics <a href="https://covaris.com/wp/wp-content/uploads/resources_pdf/pn_010542.pdf">quick guide </a>or contact <a href="/cdn-cgi/l/email-protection#3b5a4b4b5752585a4f525455484e4b4b54494f7b58544d5a49524815585456"><span class="__cf_email__" data-cfemail="472637372b2e2426332e2829343237372835330724283126352e346924282a">[email protected]</span></a>.</p> <p> </p> <p><img class="alignnone wp-image-32679 size-full" src="https://www.covaris.com/wp/wp-content/uploads/Proteomics_v7.jpg" alt="Proteomics Sample Preparation | SP3 Proteomics" width="881" height="259" /></p> </div> </div> </div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1_quote"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583730045887" > <div class="wpb_wrapper"> <p><img class="aligncenter" src="/wp/wp-content/uploads/2020/02/quote.png"" /></p> </div> </div> <div style="color: #2c2c2c;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" ><em>“AFA based sonication with SP3 provides a very streamlined, end-to-end workflow for proteomic sample preparation all the way from tissue to peptides with minimal manual intervention, establishing a really standardized workflow with low CVs. It accepts low amounts of samples producing quite nice mass spec data. This can be done from any type of tissue, either fresh frozen, FFPE or cell pellets, in a reproducible manner.”</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Professor Jeroen Krijgsveld Division head - Proteomics of Stem Cells and Cancer</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Deutsches Krebsforschungszentrum (DKFZ)</em></div></div></div></div></div><div id="SP3" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h2 vc_custom_1714644680231" >A Workflow for Formalin Fixed Paraffin Embedded Scrolls using AFA and SP3 Magnetic Beads</div> <div class="wpb_text_column wpb_content_element vc_custom_1729079542956 h6" > <div class="wpb_wrapper"> <p>As in the previous workflow, samples are processed in a single Covaris vessel. This workflow has been designed and optimized in the AFA-TUBE™ TPX plate. It consists of a first step of paraffin removal without any solvent and this unique AFA-assisted emulsification is patented. Then the sample is reverse cross-linked before a second homogenization step under AFA.</p> <p> </p> <p><img class="alignnone wp-image-32434 size-full" src="https://www.covaris.com/wp/wp-content/uploads/Proteomics_v3.jpg" alt="proteomics applications | proteomics workflow | proteomic analysis" width="661" height="496" /></p> <p>This workflow uses the <a href="https://www.covaris.com/truxtrac-proteins-tissue-lysis-buffer">TLB buffer</a> and has been specifically developed in collaboration with Matthias Mann's research groups for FFPE scrolls, read more in our <u><a href="https://www.covaris.com/wp/wp-content/uploads/2020/06/M020141.pdf">Application Note. </a></u>It works with magnetic beads for downstream clean-up, similarly to the above SP3 protocol. To learn more about developing these protocols, contact <u><a href="/cdn-cgi/l/email-protection#95f4e5e5f9fcf6f4e1fcfafbe6e0e5e5fae7e1d5f6fae3f4e7fce6bbf6faf8"><span class="__cf_email__" data-cfemail="5839282834313b392c3137362b2d2828372a2c183b372e392a312b763b3735">[email protected]</span></a></u>.</p> </div> </div> </div></div></div></div><div id="lcm" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h2 vc_custom_1714646410503" >Automated Collection and Processing of Laser Capture Microdissection using AFA and Magnetic Beads</div> <div class="wpb_text_column wpb_content_element vc_custom_1729079717872 h6" > <div class="wpb_wrapper"> <p>AFA can also be used with delicate samples like LCM. It works in AFA-TUBE TPX plate or strips and thus is directly compatible with SP3 if clean-up is used.</p> <p>When needed, slides will be first deparaffinized with an appropriate method. Then the regions of interest are selected, laser dissected and <b>directly transferred into the Covaris plate</b>. This procedure can be automated and samples collected directly in the plate when working <a href="https://downloads.leica-microsystems.com/Leica%20LMD7/Brochures/Leica_LMD6_7-TechnicalBrochure_en_2020.10.26.pdf">with a Leica LMD6 or 7</a>, using the corresponding rack. Solutions also exist for collection and processing in a strip. Please contact <a href="/cdn-cgi/l/email-protection#7a1b0a0a1613191b0e131514090f0a0a15080e3a19150c1b08130954191517"><span class="__cf_email__" data-cfemail="5938292935303a382d3036372a2c2929362b2d193a362f382b302a773a3634">[email protected]</span></a> for more information.</p> <p>After cell lysis with AFA treatment, the samples can be cleaned-up using SP3 like in the previous example. Other methods like S-Trap can also be used [4].</p> <p> </p> <p><img class="alignnone wp-image-32460 size-full" src="https://www.covaris.com/wp/wp-content/uploads/LC-MS-AFA-V2.jpg" alt="s-trap proteomics | SP3 clean up | proteomics instruments" width="1920" height="1080" /></p> <p style="text-align: center;">Adapted from Grosserueschkamp <em>et al</em>., 2017</p> </div> </div> </div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1_quote"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583730045887" > <div class="wpb_wrapper"> <p><img class="aligncenter" src="/wp/wp-content/uploads/2020/02/quote.png"" /></p> </div> </div> <div style="color: #2c2c2c;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" ><em>“We have tested this specific platform extensively in our laboratory at the Max Planck Institute of Biochemistry, Munich. Using our materials and setup, we can now prepare hundreds of biological and clinical samples in a highly streamlined manner.”</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Professor Matthias Mann Director at the Max Planck Institute of Biochemistry</em></div><p style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Head of the Clinical Proteomics group at the Novo Nordisk Center for Protein Research</em></p></div></div></div></div><div id="strap" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Formalin Fixed Paraffin Embedded (FFPE) Scrolls using AFA and S-Trap</h2> <div class="wpb_text_column wpb_content_element vc_custom_1729079814684 h6" > <div class="wpb_wrapper"> <p>Our <a href="https://www.covaris.com/ffpe-extraction/">truXTRAC FFPE</a> products offer solutions for DNA, RNA and protein extraction. For proteomics applications, scrolls (or curls) are lysed in a <a href="https://www.covaris.com/products-services/consumables">Covaris tube</a> with 5% SDS. The combination of AFA and SDS will streamline paraffin removal and emulsification while efficiently solubilizing all types of proteins. Decrosslinking is performed in the same tube. When the last AFA step is performed, the sample is transferred onto the an S-Trap column, where proteins are reduced and digested. Due to the particular design of the column, SDS is fully removed prior to MS analysis.</p> <p> </p> <p><a class="image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/06/FFPE_Protein-1024x494.jpg"><img class="alignnone wp-image-27687 size-large" src="https://www.covaris.com/wp/wp-content/uploads/2020/06/FFPE_Protein-1024x494.jpg" alt="Diagram showing Formalin Fixed Paraffin Embedded (FFPE) Scrolls using AFA and S-Trap" width="1024" height="494" /></a></p> </div> </div> </div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1_quote"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583730045887" > <div class="wpb_wrapper"> <p><img class="aligncenter" src="/wp/wp-content/uploads/2020/02/quote.png"" /></p> </div> </div> <div style="color: #2c2c2c;text-align: center" class="vc_custom_heading vc_do_custom_heading h5" ><em>“Deparaffinization of FFPE tissue in the Covaris E220 allows us to avoid the use of xylene in our small laboratory space. Paraffin is efficiently removed from FFPE samples, removing any concerns of clogging columns, contaminating instrumentation or suppressing ionization.”</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Dr. Jessica Chapman</em></div><div style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading h6" ><em>Laboratory Director, Memorial Sloan Kettering</em></div></div></div></div></div><div id="trypsin" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Enhanced trypsin digestion using AFA® for improved recovery of membrane proteins</h2> <div class="wpb_text_column wpb_content_element vc_custom_1729079919904 h6" > <div class="wpb_wrapper"> <p>AFA can be used for highly controlled dissociation of membranes followed by enhanced digestion of the membrane-associated proteins. To learn more about developing this protocol, contact<a href="/cdn-cgi/l/email-protection#620312120e0b0103160b0d0c111712120d101622010d1403100b114c010d0f"> <span class="__cf_email__" data-cfemail="462736362a2f2527322f2928353336362934320625293027342f356825292b">[email protected]</span></a></p> <p> </p> <p><img class="alignnone wp-image-32924 size-full" src="https://www.covaris.com/wp/wp-content/uploads/Proteomics_v2.jpg" alt="Diagram showing highly controlled dissociation of membranes with AFA technology" width="744" height="185" /></p> </div> </div> </div></div></div></div><div id="other" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" id="downstream">AFA for other proteomics downstream applications</h2> <div class="wpb_text_column wpb_content_element vc_custom_1714641371332 h6" > <div class="wpb_wrapper"> <p>AFA has shown to be efficient in a wide variety of downstream analytical methods with different starting materials. Plants, bacteria, yeast, and hard mammalian tissue like muscle have been successfully processed with Focused-ultrasonicators. The controlled and precise energy delivery makes AFA perfect for the preparation and preservation of low abundance or fragile subpopulations of proteins like phosphoproteins[68] <span class="cf0">, or for native protein extraction [9].</span></p> <p>Some examples are given below - please refer to our <a href="https://covaris.com/wp/wp-content/uploads/resources_pdf/M020103.pdf" target="_blank" rel="noopener noreferrer">Cell Lysis Brochure</a> for a more comprehensive overview, including extraction of other molecules of interest like metabolites or lipids.</p> </div> </div> <div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h4 vc_custom_1714646711685" id="proteinExtraction">1. Protein Extraction and Processing for SDS-PAGE and Antibody-based Assays</div> <div class="wpb_text_column wpb_content_element vc_custom_1714641743362 h6" > <div class="wpb_wrapper"> <p>For SDS-PAGE applications, including western blotting, cells can be processed as already described or collected directly in gel loading buffers like Laemmli and transferred to AFA vessels for acoustic disruption. The process can be adapted to work for different volumes based on the starting material (6-well to 96-well plates). The resulting lysate can then be loaded directly onto the gel. For ELISA and other antibody-based multiplexed assays, cells can be lysed in aqueous buffers or detergent free buffers, for full compatibility with the required downstream reagents.</p> </div> </div> <div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h4 vc_custom_1714646655540" id="plants">2. Plants, Yeasts, Bacteria and other non-mammalian sample types</div> <div class="wpb_text_column wpb_content_element vc_custom_1714641756653 h6" > <div class="wpb_wrapper"> <p>Sample preparation is always about optimization and there are a significant number of parameters that can affect the efficiency of protein recovery. In addition, some organisms have very rigid membrane constituents, others can have a cell wall on top of their membrane, and the insolubility of some components can drastically decrease the quantity of desired biomolecules. AFA has been shown efficiently process difficult materials such as plants [7,8,10], bacteria [11,12] or yeast [13,14].</p> <h5></h5> </div> </div> </div></div></div></div></div></div></div></div><div id="featured_product" class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1583494558716"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h2 vc_custom_1714646245711" >Featured Products</div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583737926712" > <div class="wpb_wrapper"> <p><img class="alignnone size-full wp-image-15216" src="https://www.covaris.com/wp/wp-content/uploads/2020/02/LE220R-plus.png" alt="" width="200" height="155" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h3 vc_custom_1714641889984" >LE220R-plus Focused-ultrasonicator</div><div style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading h6" >High-throughput, 1 to 96 tube capacity Focused-ultrasonicator with opening for robotic arm powered by Covaris AFA technology.</div><div class="vc_btn3-container vc_btn3-inline vc_custom_1583737887236 vc_do_btn" ><a style="background-color:#ededed; 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color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="https://d24ci5y4j5ezt1.cloudfront.net/wp/wp-content/uploads/resources_pdf/m020156.pdf" title="">LEARN MORE</a></div></div></div></div></div></div></div></div></div><div id="references" class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1587016866357"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1583492989135"><div class="wpb_wrapper"><div style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading h1 vc_custom_1714639431512" >References</div><div class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1587016276581"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1618246760901 custom_h5" > <div class="wpb_wrapper"> <ol> <li>Ultrasensitive proteome analysis using paramagnetic bead technology. CS Hughes et al., Mol Syst Biol. 2014;10:757. DOI: <u><a href="https://www.embopress.org/doi/full/10.15252/msb.20145625">10.15252/msb.</a></u><u><a href="https://www.embopress.org/doi/full/10.15252/msb.20145625">20145625</a></u></li> <li>Automated sample preparation with SP3 for low-input clinical proteomics. Mueller <i>et al.,</i> Mol Syst Biol (2020)16:e9111. DOI: 10.15252/msb.20199111</li> <li>A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease, Niu et al., biorxiv 2020. DOI: 10.1101/2020.10.16.337592</li> <li>Laser capture microdissection coupled mass spectrometry (LCM-MS) for spatially resolved analysis of formalin-fixed and stained human lung tissues. Herrera <i>et al.,</i> <i>Clin </i><i>Proteom</i><i> (2020) 17:24 DOI: https://doi.org/10.1186/s12014-020-09287-6</i></li> <li>HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics, Marchione et al., J. Proteome Res. 2020, 19, 2, 973–983. DOI: 10.1021/acs.jproteome.9b00686</li> <li>Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV. Stukalov <i>et al.</i> , bioRxiv 2020 - <u><a href="https://doi.org/10.1101/2020.06.17.156455">https://doi.org/10.1101/2020.06.17.156455</a></u></li> <li>Probing the global kinome and phosphoproteome in Chlamydomonas reinhardtii via sequential enrichment and quantitative proteomics. E Werth et al., The Plant Journal (2017) 89, 416–426 DOI:<u><a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13384"> 10.1111/tpj.13384</a></u></li> <li>The phosphorylated redox proteome of Chlamydomonas reinhardtii: Revealing novel means for regulation of protein structure and function. McConnell et al., Redox Biology Volume 17, July 2018, Pages 35-46 DOI: <u><a href="https://doi.org/10.1016/j.redox.2018.04.003">doi.org/10.1016/j.redox.2018.04.003</a></u></li> <li>A high-efficiency cellular extraction system for biological proteomics. Dhabaria et al., J of Proteome Res. 2015 August 7; 14(8): 3403–340 DOI: 10.1021/acs.jproteome.5b00547</li> <li>Acoustic Technology for High-Performance Disruption and Extraction of Plant Proteins. M Toorchi et al., Journal of Proteome Research 2008, 7, 3035–3041. <u><a href="https://pubs.acs.org/doi/abs/10.1021/pr800012c">DOI: 10.1021/pr800012c</a></u></li> <li>The Role of Cadaverine Synthesis on Pneumococcal Capsule and Protein Expression MF Nakamya et al., Med Sci (Basel). 2018 Jan 19;6(1).<u><a href="https://www.ncbi.nlm.nih.gov/pubmed/29351189"> DOI: 10.3390/medsci6010008</a></u></li> <li>An ultra scale-down approach to study the interaction of fermentation, homogenisation and centrifugation for antibody fragment recovery from rec E. coli. Q Li et al., Biotechnology and Bioengineering, 2013 Aug;110(8):2150-60<u><a href="https://europepmc.org/article/med/23475508"> DOI: 10.1002/bit.24891</a></u></li> <li>A Microscale Yeast Cell Disruption Technique for Integrated Process Development Strategies. MD Wenger et al., Biotechnol. Prog. 2008, 24, 606−614. <u><a href="https://aiche.onlinelibrary.wiley.com/doi/full/10.1021/bp070359s">DOI:10.1021/bp070359s</a></u></li> <li>Development of a high-throughput microscale cell disruption platform for Pichia pastoris in rapid bioprocess design. 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