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class="content-sidebar-wrap"><main class="content"><article class="post-15430 post type-post status-publish format-standard has-post-thumbnail category-forensics tag-forensics entry" aria-label="Pushing the Boundaries in Forensic DNA Analysis with NGS"><header class="entry-header"><h1 class="entry-title">Pushing the Boundaries in Forensic DNA Analysis with NGS</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/behind-the-bench-staff/" title="Posts by Behind The Bench Staff" rel="author">Behind The Bench Staff</a> </span> <span class="post-info-date"> 09.13.2021 </span> </header><div class="entry-content"><p><a href="https://events.labroots.com/event/hids-2021/en-us#!/Home" target="_blank" rel="noopener"><span class="s1">HIDS virtual 2021</span></a> showcased two presentations using NGS in forensic applications. NGS can help you solve those tough cases, and cold cases, by getting more information from challenging samples. <a href="https://events.labroots.com/event/hids-2021/en-us#!/Forensic_Lab" target="_blank" rel="noopener"><span class="s1">Hear from these two groups</span></a> on how they are developing these applications.</p> <p><strong>Forensic Phenotyping Challenged by Single-Cell Genomics</strong></p> <p><img decoding="async" src="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2021/09/pushing20the20boundaries-3.png" style="margin: 5px" alt="A graphic shows the challenges of single-cell genomics" title="Pushing the Boundaries in Forensic DNA analysis with NGS" width="1000" height="584" /></p> <p>Single cell sequencing is not commonly used in forensics. The team at the University of Munich tested groups of 5, 10 and 20 cells and performed ancestry and phenotype predictions based on consensus profiles generated with the community designed Ion AmpliSeq PhenoTrivium Panel. <a href="https://events.labroots.com/event/hids-2021/en-us/contents/684232/share?" target="_blank" rel="noopener"><span class="s1">In this presentation, Marta Diepenbroek discusses the results<span class="Apple-converted-space"></span></span></a> that suggest a great potential of using single cell sequencing in deconvoluting mixed traces submitted for forensic phenotyping.</p> <p><strong>The Whys and Why-Nots of Body Fluid Identification</strong></p> <p><img decoding="async" src="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2021/09/the20whys20and20why20nots20-3.png" style="margin: 5px" alt="A graphic shows the challenges of body fluid identification" title="The Whys and Why Nots of Body Fluid Identification" width="1000" height="584" /></p> <p><a href="https://events.labroots.com/event/hids-2021/en-us/contents/684230/share?" target="_blank" rel="noopener"><span class="s1">This informal discussion with Jack Ballantyne</span></a> from the University of Central Florida, explores why <span class="s2">Body Fluid Identification (</span>BFID) has an important role to play in forensic investigations, especially in providing some context as to how a DNA profile could (or could not) have been deposited at the crime scene and might continue to have applications in triaging evidence items for DNA testing. The principal subject of the discussion is mRNA profiling via NGS, but other next-gen BFID methods will be mentioned. Case scenarios will be used to exemplify how mRNA profiling for BFID could be used in real cases, indicating the reasons why CE or NGS based methods would be recommended.<span class="Apple-converted-space"> </span></p> <p>You can <a href="https://www.thermofisher.com/us/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis/next-generation-sequencing-ngs-forensics.html" target="_blank" rel="noopener"><span class="s3">learn more</span></a> about targeted NGS solutions using the Precision ID System for HID and how these technologies can assist in your most challenging cases.</p> <p>For research, forensic or paternity use only. 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