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variants</title> <meta name="description" content="Search results for: non-coding variants"> <meta name="keywords" content="non-coding variants"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="non-coding variants"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 343</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: non-coding variants</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">343</span> Identification of Genomic Mutations in Prostate Cancer and Cancer Stem Cells By Single Cell RNAseq Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wen-Yang%20Hu">Wen-Yang Hu</a>, <a href="https://publications.waset.org/abstracts/search?q=Ranli%20Lu"> Ranli Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Maienschein-Cline"> Mark Maienschein-Cline</a>, <a href="https://publications.waset.org/abstracts/search?q=Danping%20Hu"> Danping Hu</a>, <a href="https://publications.waset.org/abstracts/search?q=Larisa%20Nonn"> Larisa Nonn</a>, <a href="https://publications.waset.org/abstracts/search?q=Toshi%20Shioda"> Toshi Shioda</a>, <a href="https://publications.waset.org/abstracts/search?q=Gail%20S.%20Prins"> Gail S. Prins</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Genetic mutations are highly associated with increased prostate cancer risk. In addition to whole genome sequencing, somatic mutations can be identified by aligning transcriptome sequences to the human genome. Here we analyzed bulk RNAseq and single cell RNAseq data of human prostate cancer cells and their matched non-cancer cells in benign regions from 4 individual patients. Methods: Sequencing raw reads were aligned to the reference genome hg38 using STAR. Variants were annotated using Annovar with respect to overlap gene annotation information, effect on gene and protein sequence, and SIFT annotation of nonsynonymous variant effect. We determined cancer-specific novel alleles by comparing variant calls in cancer cells to matched benign cells from the same individual by selecting unique alleles that were only detected in the cancer samples. Results: In bulk RNAseq data from 3 patients, the most common variants were the noncoding mutations at UTR3/UTR5, and the major variant types were single-nucleotide polymorphisms (SNP) including frameshift mutations. C>T transversion is the most frequently presented substitution of SNP. A total of 222 genes carrying unique exonic or UTR variants were revealed in cancer cells across 3 patients but not in benign cells. Among them, transcriptome levels of 7 genes (CITED2, YOD1, MCM4, HNRNPA2B1, KIF20B, DPYSL2, NR4A1) were significantly up or down regulated in cancer stem cells. Out of the 222 commonly mutated genes in cancer, 19 have nonsynonymous variants and 11 are damaged genes with variants including SIFT, frameshifts, stop gain/loss, and insertions/deletions (indels). Two damaged genes, activating transcription factor 6 (ATF6) and histone demethylase KDM3A are of particular interest; the former is a survival factor for certain cancer cells while the later positively activates androgen receptor target genes in prostate cancer. Further, single cell RNAseq data of cancer cells and their matched non-cancer benign cells from both primary 2D and 3D tumoroid cultures were analyzed. Similar to the bulk RNAseq data, single cell RNAseq in cancer demonstrated that the exonic mutations are less common than noncoding variants, with SNPs including frameshift mutations the most frequently presented types in cancer. Compared to cancer stem cell enriched-3D tumoroids, 2D cancer cells carried 3-times higher variants, 8-times more coding mutations and 10-times more nonsynonymous SNP. Finally, in both 2D primary and 3D tumoroid cultures, cancer stem cells exhibited fewer coding mutations and noncoding SNP or insertions/deletions than non-stem cancer cells. Summary: Our study demonstrates the usefulness of bulk and single cell RNAseaq data in identifying somatic mutations in prostate cancer, providing an alternative method in screening candidate genes for prostate cancer diagnosis and potential therapeutic targets. Cancer stem cells carry fewer somatic mutations than non-stem cancer cells due to their inherited immortal stand DNA from parental stem cells that explains their long-lived characteristics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=prostate%20cancer" title="prostate cancer">prostate cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=stem%20cell" title=" stem cell"> stem cell</a>, <a href="https://publications.waset.org/abstracts/search?q=genomic%20mutation" title=" genomic mutation"> genomic mutation</a>, <a href="https://publications.waset.org/abstracts/search?q=RNAseq" title=" RNAseq"> RNAseq</a> </p> <a href="https://publications.waset.org/abstracts/193081/identification-of-genomic-mutations-in-prostate-cancer-and-cancer-stem-cells-by-single-cell-rnaseq-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193081.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">18</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">342</span> Role of Long Noncoding RNA HULC on Colorectal Carcinoma Progression through Epigenetically Repressing NKD2 Expression</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shu-Jun%20Li">Shu-Jun Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheng-Cao%20Sun"> Cheng-Cao Sun</a>, <a href="https://publications.waset.org/abstracts/search?q=De-Jia%20Li"> De-Jia Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recently, long noncoding RNAs (lncRNAs) have been emerged as crucial regulators of human diseases and prognostic markers in numerous of cancers, including colorectal carcinoma (CRC). Here, we identified an oncogenetic lncRNA HULC, which may promote colorectal tumorigenesis. HULC has been found to be up-regulated and acts as oncogene in gastric cancer and hepatocellular carcinoma, but its expression pattern, biological function and underlying mechanism in CRC is still undetermined. Here, we reported that HULC expression is also over-expressed in CRC, and its increased level is associated with poor prognosis and shorter survival. Knockdown of HULC impaired CRC cells proliferation, migration and invasion, facilitated cell apoptosis in vitro, and inhibited tumorigenicity of CRC cells in vivo. Mechanistically, RNA immunoprecipitation (RIP) and RNA pull-down experiment demonstrated that HULC could simultaneously interact with EZH2 to repress underlying targets NKD2 transcription. In addition, rescue experiments determined that HULC oncogenic function is partly dependent on repressing NKD2. Taken together, our findings expound how HULC over-expression endows an oncogenic function in CRC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=long%20noncoding%20RNA" title="long noncoding RNA">long noncoding RNA</a>, <a href="https://publications.waset.org/abstracts/search?q=HULC" title=" HULC"> HULC</a>, <a href="https://publications.waset.org/abstracts/search?q=NKD2" title=" NKD2"> NKD2</a>, <a href="https://publications.waset.org/abstracts/search?q=colorectal%20carcinoma" title=" colorectal carcinoma"> colorectal carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=proliferation" title=" proliferation"> proliferation</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a> </p> <a href="https://publications.waset.org/abstracts/54941/role-of-long-noncoding-rna-hulc-on-colorectal-carcinoma-progression-through-epigenetically-repressing-nkd2-expression" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54941.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">225</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">341</span> Systematic Identification of Noncoding Cancer Driver Somatic Mutations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zohar%20Manber">Zohar Manber</a>, <a href="https://publications.waset.org/abstracts/search?q=Ran%20Elkon"> Ran Elkon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Accumulation of somatic mutations (SMs) in the genome is a major driving force of cancer development. Most SMs in the tumor's genome are functionally neutral; however, some cause damage to critical processes and provide the tumor with a selective growth advantage (termed cancer driver mutations). Current research on functional significance of SMs is mainly focused on finding alterations in protein coding sequences. However, the exome comprises only 3% of the human genome, and thus, SMs in the noncoding genome significantly outnumber those that map to protein-coding regions. Although our understanding of noncoding driver SMs is very rudimentary, it is likely that disruption of regulatory elements in the genome is an important, yet largely underexplored mechanism by which somatic mutations contribute to cancer development. The expression of most human genes is controlled by multiple enhancers, and therefore, it is conceivable that regulatory SMs are distributed across different enhancers of the same target gene. Yet, to date, most statistical searches for regulatory SMs have considered each regulatory element individually, which may reduce statistical power. The first challenge in considering the cumulative activity of all the enhancers of a gene as a single unit is to map enhancers to their target promoters. Such mapping defines for each gene its set of regulating enhancers (termed "set of regulatory elements" (SRE)). Considering multiple enhancers of each gene as one unit holds great promise for enhancing the identification of driver regulatory SMs. However, the success of this approach is greatly dependent on the availability of comprehensive and accurate enhancer-promoter (E-P) maps. To date, the discovery of driver regulatory SMs has been hindered by insufficient sample sizes and statistical analyses that often considered each regulatory element separately. In this study, we analyzed more than 2,500 whole-genome sequence (WGS) samples provided by The Cancer Genome Atlas (TCGA) and The International Cancer Genome Consortium (ICGC) in order to identify such driver regulatory SMs. Our analyses took into account the combinatorial aspect of gene regulation by considering all the enhancers that control the same target gene as one unit, based on E-P maps from three genomics resources. The identification of candidate driver noncoding SMs is based on their recurrence. We searched for SREs of genes that are "hotspots" for SMs (that is, they accumulate SMs at a significantly elevated rate). To test the statistical significance of recurrence of SMs within a gene's SRE, we used both global and local background mutation rates. Using this approach, we detected - in seven different cancer types - numerous "hotspots" for SMs. To support the functional significance of these recurrent noncoding SMs, we further examined their association with the expression level of their target gene (using gene expression data provided by the ICGC and TCGA for samples that were also analyzed by WGS). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cancer%20genomics" title="cancer genomics">cancer genomics</a>, <a href="https://publications.waset.org/abstracts/search?q=enhancers" title=" enhancers"> enhancers</a>, <a href="https://publications.waset.org/abstracts/search?q=noncoding%20genome" title=" noncoding genome"> noncoding genome</a>, <a href="https://publications.waset.org/abstracts/search?q=regulatory%20elements" title=" regulatory elements "> regulatory elements </a> </p> <a href="https://publications.waset.org/abstracts/121517/systematic-identification-of-noncoding-cancer-driver-somatic-mutations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/121517.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">340</span> Identification of COVID-SARS Variants Based on Lactate Test Results</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zoltan%20Horvath">Zoltan Horvath</a>, <a href="https://publications.waset.org/abstracts/search?q=Dora%20Nagy"> Dora Nagy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this research, it was examined whether individual COVID variants cause differences in the lactate curve of cyclists. After all, the virus variants attacked different organs in our body during the infections. During our tests, we used a traditional lactate step test, the results of which were compared with the values before the infection. In the tests, it has been proven that different virus variants show unique lactate curves. In this way, based on the lactate curve, it is possible to identify which variant caused the disease. Thanks to this, it has been shorten the return time, because we can apply the best return protocol after infection to the competitors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=COVID-Sars19" title="COVID-Sars19">COVID-Sars19</a>, <a href="https://publications.waset.org/abstracts/search?q=lactate" title=" lactate"> lactate</a>, <a href="https://publications.waset.org/abstracts/search?q=virus%20mutation" title=" virus mutation"> virus mutation</a>, <a href="https://publications.waset.org/abstracts/search?q=lactate%20profile" title=" lactate profile"> lactate profile</a> </p> <a href="https://publications.waset.org/abstracts/171056/identification-of-covid-sars-variants-based-on-lactate-test-results" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171056.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">66</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">339</span> Production Plan and Technological Variants Optimization by Goal Programming Methods</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tunjo%20Peri%C4%87">Tunjo Perić</a>, <a href="https://publications.waset.org/abstracts/search?q=Franjo%20Brati%C4%87"> Franjo Bratić</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper the goal programming methodology for solving multiple objective problem of the technological variants and production plan optimization has been applied. The optimization criteria are determined and the multiple objective linear programming model for solving a problem of the technological variants and production plan optimization is formed and solved. Then the obtained results are analysed. The obtained results point out to the possibility of efficient application of the goal programming methodology in solving the problem of the technological variants and production plan optimization. The paper points out on the advantages of the application of the goal programming methodolohy compare to the Surrogat Worth Trade-off method in solving this problem. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=goal%20programming" title="goal programming">goal programming</a>, <a href="https://publications.waset.org/abstracts/search?q=multi%20objective%20programming" title=" multi objective programming"> multi objective programming</a>, <a href="https://publications.waset.org/abstracts/search?q=production%20plan" title=" production plan"> production plan</a>, <a href="https://publications.waset.org/abstracts/search?q=SWT%20method" title=" SWT method"> SWT method</a>, <a href="https://publications.waset.org/abstracts/search?q=technological%20variants" title=" technological variants"> technological variants</a> </p> <a href="https://publications.waset.org/abstracts/31123/production-plan-and-technological-variants-optimization-by-goal-programming-methods" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31123.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">379</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">338</span> Determination of Strain Rate Sensitivity (SRS) for Grain Size Variants on Nanocrystalline Materials Produced by ARB and ECAP</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20B.%20Sob">P. B. Sob</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20B.%20Tengen"> T. B. Tengen</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Alugongo"> A. A. Alugongo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mechanical behavior of 6082T6 aluminum is investigated at different temperatures. The strain rate sensitivity is investigated at different temperatures on the grain size variants. The sensitivity of the measured grain size variants on 3-D grain is discussed. It is shown that the strain rate sensitivities are negative for the grain size variants during the deformation of nanostructured materials. It is also observed that the strain rate sensitivities vary in different ways with the equivalent radius, semi minor axis radius, semi major axis radius and major axis radius. From the obtained results, it is shown that the variation of strain rate sensitivity with temperature suggests that the strain rate sensitivity at the low and the high temperature ends of the 6082T6 aluminum range is different. The obtained results revealed transition at different temperature from negative strain rate sensitivity as temperature increased on the grain size variants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanostructured%20materials" title="nanostructured materials">nanostructured materials</a>, <a href="https://publications.waset.org/abstracts/search?q=grain%20size%20variants" title=" grain size variants"> grain size variants</a>, <a href="https://publications.waset.org/abstracts/search?q=temperature" title=" temperature"> temperature</a>, <a href="https://publications.waset.org/abstracts/search?q=yield%20stress" title=" yield stress"> yield stress</a>, <a href="https://publications.waset.org/abstracts/search?q=strain%20rate%20sensitivity" title=" strain rate sensitivity"> strain rate sensitivity</a> </p> <a href="https://publications.waset.org/abstracts/36624/determination-of-strain-rate-sensitivity-srs-for-grain-size-variants-on-nanocrystalline-materials-produced-by-arb-and-ecap" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/36624.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">287</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">337</span> Mutation Profiling of Paediatric Solid Tumours in a Cohort of South African Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=L.%20Lamola">L. Lamola</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Manolas"> E. Manolas</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Krause"> A. Krause</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The incidence of childhood cancer incidence is increasing gradually in low-middle income countries, such as South Africa. Globally, there is an extensive range of familial- and hereditary-cancer syndromes, where underlying germline variants increase the likelihood of developing cancer in childhood. Next-Generation Sequencing (NGS) technologies have been key in determining the occurrence and genetic contribution of germline variants to paediatric cancer development. We aimed to design and evaluate a candidate gene panel specific to inherited cancer-predisposing genes to provide a comprehensive insight into the contribution of germline variants to childhood cancer. Methods: 32 paediatric patients (aged 0-18 years) diagnosed with a malignant tumour were recruited, and biological samples were obtained. After quality control, DNA was sequenced using an ion Ampliseq 50 candidate gene panel design and Ion Torrent S5 technologies. Sequencing variants were called using Ion Torrent Suite software and were subsequently annotated using Ion Reporter and Ensembl's VEP. High priority variants were manually analysed using tools such as MutationTaster, SIFT-INDEL and VarSome. Putative identified candidates were validated via Sanger Sequencing. Results: The patients studied had a variety of cancers, the most common being nephroblastoma (13), followed by osteosarcoma (4) and astrocytoma (3). We identified 10 pathogenic / likely pathogenic variants in 10 patients, most of which were novel. Conclusions: According to the literature, we expected ~10% of our patient population to harbour pathogenic or likely pathogenic germline variants, however, we reported about 3 times (~30%) more than we expected. Majority of the identified variants are novel; this may be because this is the first study of its kind in an understudied South African population. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Africa" title="Africa">Africa</a>, <a href="https://publications.waset.org/abstracts/search?q=genetics" title=" genetics"> genetics</a>, <a href="https://publications.waset.org/abstracts/search?q=germline-variants" title=" germline-variants"> germline-variants</a>, <a href="https://publications.waset.org/abstracts/search?q=paediatric-cancer" title=" paediatric-cancer"> paediatric-cancer</a> </p> <a href="https://publications.waset.org/abstracts/144450/mutation-profiling-of-paediatric-solid-tumours-in-a-cohort-of-south-african-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144450.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">336</span> Effects of Drought on Microbial Activity in Rhizosphere, Soil Hydrophobicity and Leaching of Mineral Nitrogen from Arable Soil Depending on Method of Fertilization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jakub%20Elbl">Jakub Elbl</a>, <a href="https://publications.waset.org/abstracts/search?q=Luk%C3%A1%C5%A1%20Plo%C5%A1ek"> Lukáš Plošek</a>, <a href="https://publications.waset.org/abstracts/search?q=Anton%C3%ADn%20Kintl"> Antonín Kintl</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaroslav%20Hyn%C5%A1t"> Jaroslav Hynšt</a>, <a href="https://publications.waset.org/abstracts/search?q=So%C5%88a%20Javorekov%C3%A1"> Soňa Javoreková</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaroslav%20Z%C3%A1hora"> Jaroslav Záhora</a>, <a href="https://publications.waset.org/abstracts/search?q=Libor%20Kalhotka"> Libor Kalhotka</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Urb%C3%A1nkov%C3%A1"> Olga Urbánková</a>, <a href="https://publications.waset.org/abstracts/search?q=Ivana%20Charousov%C3%A1"> Ivana Charousová </a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work presents the first results from the long-term laboratory experiment dealing with impact of drought on soil properties. Three groups of the treatment (A, B and C) with different regime of irrigation were prepared. The soil water content was maintained at 70 % of soil water holding capacity in group A, at 40 % in group B. In group C, soil water regime was maintained in the range of wilting point. Each group of the experiment was divided into three variants (A1 = B1, C1; A2 = B2, C2 etc.) with three repetitions: Variants A1 (B1, C1) were controls without addition of another fertilizer. Variants A2 (B2, C2) were fertilized with mineral nitrogen fertilizer DAM 390 (0.140 Mg of N per ha) and variants A3 (B3, C3) contained 45 g of Cp per a pot. The significant differences (ANOVA, P<0.05) in the leaching of mineral nitrogen and values of saturated hydraulic conductivity (Ksat) were found. The highest values of Ksat were found in variants (within each group) with addition of compost (A3, B3, C3). Conversely, the lowest values of Ksat were found in variants with addition of mineral nitrogen. Low values of Ksat indicate an increased level of hydrophobicity in individual groups of the experiment. Moreover, all variants with compost addition showed lower amount of mineral nitrogen leaching and high level of microbial activity than variants without. This decrease of mineral nitrogen leaching was about 200 % in comparison with the control variant and about 300 % with variant, where mineral nitrogen was added. Based on these results, we can conclude that changes of soil water content directly have impact on microbial activity, soil hydrophobicity and loss of mineral nitrogen from the soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drought" title="drought">drought</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20activity" title=" microbial activity"> microbial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=mineral%20nitrogen" title=" mineral nitrogen"> mineral nitrogen</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20hydrophobicity" title=" soil hydrophobicity"> soil hydrophobicity</a> </p> <a href="https://publications.waset.org/abstracts/7657/effects-of-drought-on-microbial-activity-in-rhizosphere-soil-hydrophobicity-and-leaching-of-mineral-nitrogen-from-arable-soil-depending-on-method-of-fertilization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/7657.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">383</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">335</span> Variation in Italian Specialized Economic Texts</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdelmagid%20Basyouny%20Sakr">Abdelmagid Basyouny Sakr</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Terminological variation is a reality and it is now recognized by terminologists. This paper investigates the terminological variation in the context of specialized economic texts in Italian. It aims to find whether certain patterns or tendencies can be derived from the analysis of these texts. Term variants pose two different kinds of difficulties. The first one is being able to recognize linguistic expressions that denote the same concept in running text. Another one lies in knowing which variant should be considered and for what purpose. This would help to differentiate between variants that could be candidates for inclusion in terminological resources and the ones which are synonyms or contextual variants. New insights about terminological variation in specialized texts could contribute to improve specialized dictionaries which will better account for the different ways in which a given thought is expressed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=corpus%20linguistics" title="corpus linguistics">corpus linguistics</a>, <a href="https://publications.waset.org/abstracts/search?q=specialized%20communication" title=" specialized communication"> specialized communication</a>, <a href="https://publications.waset.org/abstracts/search?q=terms%20and%20concepts" title=" terms and concepts"> terms and concepts</a>, <a href="https://publications.waset.org/abstracts/search?q=terminological%20variation" title=" terminological variation"> terminological variation</a> </p> <a href="https://publications.waset.org/abstracts/147949/variation-in-italian-specialized-economic-texts" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147949.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">334</span> Whole Exome Sequencing Data Analysis of Rare Diseases: Non-Coding Variants and Copy Number Variations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Fahiminiya">S. Fahiminiya</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Nadaf"> J. Nadaf</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Rauch"> F. Rauch</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Jerome-Majewska"> L. Jerome-Majewska</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Majewski"> J. Majewski </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Sequencing of protein coding regions of human genome (Whole Exome Sequencing; WES), has demonstrated a great success in the identification of causal mutations for several rare genetic disorders in human. Generally, most of WES studies have focused on rare variants in coding exons and splicing-sites where missense substitutions lead to the alternation of protein product. Although focusing on this category of variants has revealed the mystery behind many inherited genetic diseases in recent years, a subset of them remained still inconclusive. Here, we present the result of our WES studies where analyzing only rare variants in coding regions was not conclusive but further investigation revealed the involvement of non-coding variants and copy number variations (CNV) in etiology of the diseases. Methods: Whole exome sequencing was performed using our standard protocols at Genome Quebec Innovation Center, Montreal, Canada. All bioinformatics analyses were done using in-house WES pipeline. Results: To date, we successfully identified several disease causing mutations within gene coding regions (e.g. SCARF2: Van den Ende-Gupta syndrome and SNAP29: 22q11.2 deletion syndrome) by using WES. In addition, we showed that variants in non-coding regions and CNV have also important value and should not be ignored and/or filtered out along the way of bioinformatics analysis on WES data. For instance, in patients with osteogenesis imperfecta type V and in patients with glucocorticoid deficiency, we identified variants in 5'UTR, resulting in the production of longer or truncating non-functional proteins. Furthermore, CNVs were identified as the main cause of the diseases in patients with metaphyseal dysplasia with maxillary hypoplasia and brachydactyly and in patients with osteogenesis imperfecta type VII. Conclusions: Our study highlights the importance of considering non-coding variants and CNVs during interpretation of WES data, as they can be the only cause of disease under investigation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=whole%20exome%20sequencing%20data" title="whole exome sequencing data">whole exome sequencing data</a>, <a href="https://publications.waset.org/abstracts/search?q=non-coding%20variants" title=" non-coding variants"> non-coding variants</a>, <a href="https://publications.waset.org/abstracts/search?q=copy%20number%20variations" title=" copy number variations"> copy number variations</a>, <a href="https://publications.waset.org/abstracts/search?q=rare%20diseases" title=" rare diseases"> rare diseases</a> </p> <a href="https://publications.waset.org/abstracts/24069/whole-exome-sequencing-data-analysis-of-rare-diseases-non-coding-variants-and-copy-number-variations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24069.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">419</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">333</span> Variants of Fat Mass Obesity Associated rs 9939609 Associated with Obesity and Eating Behavior in Adolescent of Minangkabau Ethnic</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Susmiati">Susmiati</a>, <a href="https://publications.waset.org/abstracts/search?q=Ingrid%20S.%20Surono"> Ingrid S. Surono</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamsari"> Jamsari</a>, <a href="https://publications.waset.org/abstracts/search?q=Nur%20Indrawati%20Lipoeto"> Nur Indrawati Lipoeto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There are two contradicting opinions on the relationship between fat mass obesity associated (FTO) rs 9939609 variants and obesity on various ethnics and races. The first opinion agrees that there is an association between the two variables, yet another one disagree. Minangkabau ethnic had a different dietary pattern with other ethnics in Indonesia. They had higher fat and low fiber intakes compared to the other ethnics groups. There is little research in genetic factors that influence eating behavior (food preference or food selection). The objective of this study was to investigate the association between FTO rs 9939609 variants with obesity and eating behavior in adolescent girls of Minangkabau Ethnic. The research design was case control study. A total of 275 adolescent girls aged 12-15 years old (130 obese and 145 normal) were randomly chosen from four districts at West Sumatera (Padang, Padang Pariaman, Padang Panjang and Tanah Datar). Genetic variants of FTO rs 9939609 were analyzed with Tetra-primer Amplification Refractory Mutation System-Polimerase Chain Reaction (AMRS PCR), eating behavior were gathered using eating habits questionnaire, and Body Mass Index (BMI) was calculated according to BMI Z-score (WHO). The result showed that genetic variants of FTO rs 9939609 (TT, TA and AA genotype) had associated with obesity (p = 0,013), whereas subject with An Allele was significantly associated with obesity (odds ratio 1,62 [95% confidential interval, 1,00-2,60]). Subjects with An Allele carrier reported a higher consumption of fried food (p < 0.05) as compared to TT genotypes carriers. There is no association between genetic variants and meal frequency, fruit and fiber intakes p > 0.05. The genetic variants of FTO rs 9939609 are associated with obesity and eating behavior in adolescent of Minangkabau Ethics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=FTO%20rs9939609" title="FTO rs9939609">FTO rs9939609</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=eating%20behavior" title=" eating behavior"> eating behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=adolescents" title=" adolescents"> adolescents</a> </p> <a href="https://publications.waset.org/abstracts/69118/variants-of-fat-mass-obesity-associated-rs-9939609-associated-with-obesity-and-eating-behavior-in-adolescent-of-minangkabau-ethnic" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69118.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">173</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">332</span> Biological Significance of Long Intergenic Noncoding RNA LINC00273 in Lung Cancer Cell Metastasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ipsita%20Biswas">Ipsita Biswas</a>, <a href="https://publications.waset.org/abstracts/search?q=Arnab%20Sarkar"> Arnab Sarkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashikur%20Rahaman"> Ashikur Rahaman</a>, <a href="https://publications.waset.org/abstracts/search?q=Gopeswar%20Mukherjee"> Gopeswar Mukherjee</a>, <a href="https://publications.waset.org/abstracts/search?q=Subhrangsu%20Chatterjee"> Subhrangsu Chatterjee</a>, <a href="https://publications.waset.org/abstracts/search?q=Shamee%20Bhattacharjee"> Shamee Bhattacharjee</a>, <a href="https://publications.waset.org/abstracts/search?q=Deba%20Prasad%20Mandal"> Deba Prasad Mandal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of the major reasons for the high mortality rate of lung cancer is the substantial delays in disease detection at late metastatic stages. It is of utmost importance to understand the detailed molecular signaling and detect the molecular markers that can be used for the early diagnosis of cancer. Several studies explored the emerging roles of long noncoding RNAs (lncRNAs) in various cancers as well as lung cancer. A long non-coding RNA LINC00273 was recently discovered to promote cancer cell migration and invasion, and its positive correlation with the pathological stages of metastasis may prove it to be a potential target for inhibiting cancer cell metastasis. Comparing real-time expression of LINC00273 in various human clinical cancer tissue samples with normal tissue samples revealed significantly higher expression in cancer tissues. This long intergenic noncoding RNA was found to be highly expressed in human liver tumor-initiating cells, human gastric adenocarcinoma AGS cell line, as well as human non-small cell lung cancer A549 cell line. SiRNA and shRNA-induced knockdown of LINC00273 in both in vitro and in vivo nude mice significantly subsided AGS and A549 cancer cell migration and invasion. LINC00273 knockdown also reduced TGF-β induced SNAIL, SLUG, VIMENTIN, ZEB1 expression, and metastasis in A549 cells. Plenty of reports have suggested the role of microRNAs of the miR200 family in reversing epithelial to mesenchymal transition (EMT) by inhibiting ZEB transcription factors. In this study, hsa-miR-200a-3p was predicted via IntaRNA-Freiburg RNA tools to be a potential target of LINC00273 with a negative free binding energy of −8.793 kcal/mol, and this interaction was verified as a confirmed target of LINC00273 by RNA pulldown, real-time PCR and luciferase assay. Mechanistically, LINC00273 accelerated TGF-β induced EMT by sponging hsa-miR-200a-3p which in turn liberated ZEB1 and promoted prometastatic functions in A549 cells in vitro as verified by real-time PCR and western blotting. The similar expression patterns of these EMT regulatory pathway molecules, viz. LINC00273, hsa-miR-200a-3p, ZEB1 and TGF-β, were also detected in various clinical samples like breast cancer tissues, oral cancer tissues, lung cancer tissues, etc. Overall, this LINC00273 mediated EMT regulatory signaling can serve as a potential therapeutic target for the prevention of lung cancer metastasis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=epithelial%20to%20mesenchymal%20transition" title="epithelial to mesenchymal transition">epithelial to mesenchymal transition</a>, <a href="https://publications.waset.org/abstracts/search?q=long%20noncoding%20RNA" title=" long noncoding RNA"> long noncoding RNA</a>, <a href="https://publications.waset.org/abstracts/search?q=microRNA" title=" microRNA"> microRNA</a>, <a href="https://publications.waset.org/abstracts/search?q=non-small-cell%20lung%20carcinoma" title=" non-small-cell lung carcinoma"> non-small-cell lung carcinoma</a> </p> <a href="https://publications.waset.org/abstracts/143383/biological-significance-of-long-intergenic-noncoding-rna-linc00273-in-lung-cancer-cell-metastasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143383.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">156</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">331</span> The Effect of Deformation Activation Volume, Strain Rate Sensitivity and Processing Temperature of Grain Size Variants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20B.%20Sob">P. B. Sob</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Alugongo"> A. A. Alugongo</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20B.%20Tengen"> T. B. Tengen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The activation volume of 6082T6 aluminum is investigated at different temperatures on grain size variants. The deformation activation volume was computed on the basis of the relationship between the Boltzmann’s constant k, the testing temperatures, the material strain rate sensitivity and the material yield stress of grain size variants. The material strain rate sensitivity is computed as a function of yield stress and strain rate of grain size variants. The effect of the material strain rate sensitivity and the deformation activation volume of 6082T6 aluminum at different temperatures of 3-D grain are discussed. It is shown that the strain rate sensitivities and activation volume are negative for the grain size variants during the deformation of nanostructured materials. It is also observed that the activation volume vary in different ways with the equivalent radius, semi minor axis radius, semi major axis radius and major axis radius. From the obtained results it is shown that the variation of activation volume increased and decreased with the testing temperature. It was revealed that, increased in strain rate sensitivity led to decrease in activation volume whereas increased in activation volume led to decrease in strain rate sensitivity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanostructured%20materials" title="nanostructured materials">nanostructured materials</a>, <a href="https://publications.waset.org/abstracts/search?q=grain%20size%20variants" title=" grain size variants"> grain size variants</a>, <a href="https://publications.waset.org/abstracts/search?q=temperature" title=" temperature"> temperature</a>, <a href="https://publications.waset.org/abstracts/search?q=yield%20stress" title=" yield stress"> yield stress</a>, <a href="https://publications.waset.org/abstracts/search?q=strain%20rate%20sensitivity" title=" strain rate sensitivity"> strain rate sensitivity</a>, <a href="https://publications.waset.org/abstracts/search?q=activation%20volume" title=" activation volume"> activation volume</a> </p> <a href="https://publications.waset.org/abstracts/39079/the-effect-of-deformation-activation-volume-strain-rate-sensitivity-and-processing-temperature-of-grain-size-variants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39079.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">251</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">330</span> Whole Coding Genome Inter-Clade Comparison to Predict Global Cancer-Protecting Variants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lamis%20Naddaf">Lamis Naddaf</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuval%20Tabach"> Yuval Tabach</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this research, we identified the missense genetic variants that have the potential to enhance resistance against cancer. Such field has not been widely explored, as researchers tend to investigate mutations that cause diseases, in response to the suffering of patients, rather than those mutations that protect from them. In conjunction with the genomic revolution, and the advances in genetic engineering and synthetic biology, identifying the protective variants will increase the power of genotype-phenotype predictions and can have significant implications on improved risk estimation, diagnostics, prognosis and even for personalized therapy and drug discovery. To approach our goal, we systematically investigated the sites of the coding genomes and picked up the alleles that showed a correlation with the species’ cancer resistance. We predicted 250 protecting variants (PVs) with a 0.01 false discovery rate and more than 20 thousand PVs with a 0.25 false discovery rate. Cancer resistance in Mammals and reptiles was significantly predicted by the number of PVs a species has. Moreover, Genes enriched with the protecting variants are enriched in pathways relevant to tumor suppression like pathways of Hedgehog signaling and silencing, which its improper activation is associated with the most common form of cancer malignancy. We also showed that the PVs are more abundant in healthy people compared to cancer patients within different human races. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=comparative%20genomics" title="comparative genomics">comparative genomics</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer%20resistance" title=" cancer resistance"> cancer resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer-protecting%20alleles" title="cancer-protecting alleles">cancer-protecting alleles</a> </p> <a href="https://publications.waset.org/abstracts/150807/whole-coding-genome-inter-clade-comparison-to-predict-global-cancer-protecting-variants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/150807.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">97</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">329</span> Frequent Pattern Mining for Digenic Human Traits</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Atsuko%20Okazaki">Atsuko Okazaki</a>, <a href="https://publications.waset.org/abstracts/search?q=Jurg%20Ott"> Jurg Ott</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Some genetic diseases (‘digenic traits’) are due to the interaction between two DNA variants. For example, certain forms of Retinitis Pigmentosa (a genetic form of blindness) occur in the presence of two mutant variants, one in the ROM1 gene and one in the RDS gene, while the occurrence of only one of these mutant variants leads to a completely normal phenotype. Detecting such digenic traits by genetic methods is difficult. A common approach to finding disease-causing variants is to compare 100,000s of variants between individuals with a trait (cases) and those without the trait (controls). Such genome-wide association studies (GWASs) have been very successful but hinge on genetic effects of single variants, that is, there should be a difference in allele or genotype frequencies between cases and controls at a disease-causing variant. Frequent pattern mining (FPM) methods offer an avenue at detecting digenic traits even in the absence of single-variant effects. The idea is to enumerate pairs of genotypes (genotype patterns) with each of the two genotypes originating from different variants that may be located at very different genomic positions. What is needed is for genotype patterns to be significantly more common in cases than in controls. Let Y = 2 refer to cases and Y = 1 to controls, with X denoting a specific genotype pattern. We are seeking association rules, ‘X → Y’, with high confidence, P(Y = 2|X), significantly higher than the proportion of cases, P(Y = 2) in the study. Clearly, generally available FPM methods are very suitable for detecting disease-associated genotype patterns. We use fpgrowth as the basic FPM algorithm and built a framework around it to enumerate high-frequency digenic genotype patterns and to evaluate their statistical significance by permutation analysis. Application to a published dataset on opioid dependence furnished results that could not be found with classical GWAS methodology. There were 143 cases and 153 healthy controls, each genotyped for 82 variants in eight genes of the opioid system. The aim was to find out whether any of these variants were disease-associated. The single-variant analysis did not lead to significant results. Application of our FPM implementation resulted in one significant (p < 0.01) genotype pattern with both genotypes in the pattern being heterozygous and originating from two variants on different chromosomes. This pattern occurred in 14 cases and none of the controls. Thus, the pattern seems quite specific to this form of substance abuse and is also rather predictive of disease. An algorithm called Multifactor Dimension Reduction (MDR) was developed some 20 years ago and has been in use in human genetics ever since. This and our algorithms share some similar properties, but they are also very different in other respects. The main difference seems to be that our algorithm focuses on patterns of genotypes while the main object of inference in MDR is the 3 × 3 table of genotypes at two variants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=digenic%20traits" title="digenic traits">digenic traits</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20variants" title=" DNA variants"> DNA variants</a>, <a href="https://publications.waset.org/abstracts/search?q=epistasis" title=" epistasis"> epistasis</a>, <a href="https://publications.waset.org/abstracts/search?q=statistical%20genetics" title=" statistical genetics"> statistical genetics</a> </p> <a href="https://publications.waset.org/abstracts/135746/frequent-pattern-mining-for-digenic-human-traits" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/135746.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">122</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">328</span> The Impact of P108L Genetic Variant on Calcium Release and Malignant Hyperthermia Susceptibility</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20Althobiti">Mohammed Althobiti</a>, <a href="https://publications.waset.org/abstracts/search?q=Patrick%20Booms"> Patrick Booms</a>, <a href="https://publications.waset.org/abstracts/search?q=Dorota%20Fiszer"> Dorota Fiszer</a>, <a href="https://publications.waset.org/abstracts/search?q=Philip%20Hopkins"> Philip Hopkins</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Malignant hyperthermia (MH) is a pharmacogenetic disorder of skeletal muscle. MH results from anaesthetics induced breakdown of calcium homeostasis. RYR1 and CACN1AS mutations represent the aetiology in ~70% of the MH population. Previous studies indicate that up to 25% of MH patients carry no variants in these genes. Therefore, the aim of this study is to investigate the relationships between MH susceptibility and genes encoding skeletal muscle Ca2+ channels as well as accessory proteins. The JSRP, encoding JP-45, was previously sequenced and novel genetic variants were identified. The variant p.P108L (c.323C > T) was identified in exon 4 and encodes a change from a proline at amino acid 108 to leucine residue. The variant P108L was detected in two patients out of 50 with 4% frequency in the sample population. The alignment of DNA sequences in different species indicates highly conserved proline sequences involved in the substitution of the P108L variant. In this study, the variant P108L co-segregates with the SNP p.V92A (c.275T > C) at the same exon, both variants being inherited in the same two patients only. This indicates that the two variants may represent a haplotype. Therefore, a set of single nucleotide polymorphisms and statistical analysis will be used to investigate the effects of haplotypes on MH susceptibility. Furthermore, investigating the effect of the P108L variant in combination with RYR1 mutations or other genetic variants in other genes as a combination of two or more genetic variants, haplotypes may then provide stronger genetic evidence indicating that JSRP1 is associated with MH susceptibility. In conclusion, these preliminary results lend a potential modifier role of the variant P108L in JSRP1 in MH susceptibility and further investigations are suggested to confirm these results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=JSRP1" title="JSRP1">JSRP1</a>, <a href="https://publications.waset.org/abstracts/search?q=malignant%20hyperthermia" title=" malignant hyperthermia"> malignant hyperthermia</a>, <a href="https://publications.waset.org/abstracts/search?q=RyR1" title=" RyR1"> RyR1</a>, <a href="https://publications.waset.org/abstracts/search?q=skeletal%20muscle" title=" skeletal muscle"> skeletal muscle</a> </p> <a href="https://publications.waset.org/abstracts/35641/the-impact-of-p108l-genetic-variant-on-calcium-release-and-malignant-hyperthermia-susceptibility" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35641.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">335</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">327</span> Copy Number Variants in Children with Non-Syndromic Congenital Heart Diseases from Mexico</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maria%20Lopez-Ibarra">Maria Lopez-Ibarra</a>, <a href="https://publications.waset.org/abstracts/search?q=Ana%20Velazquez-Wong"> Ana Velazquez-Wong</a>, <a href="https://publications.waset.org/abstracts/search?q=Lucelli%20Ya%C3%B1ez-Gutierrez"> Lucelli Yañez-Gutierrez</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Araujo-Solis"> Maria Araujo-Solis</a>, <a href="https://publications.waset.org/abstracts/search?q=Fabio%20Salamanca-Gomez"> Fabio Salamanca-Gomez</a>, <a href="https://publications.waset.org/abstracts/search?q=Alfonso%20Mendez-Tenorio"> Alfonso Mendez-Tenorio</a>, <a href="https://publications.waset.org/abstracts/search?q=Hayde%C3%A9%20Rosas-Vargas"> Haydeé Rosas-Vargas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Congenital heart diseases (CHD) are the most common congenital abnormalities. These conditions can occur as both an element of distinct chromosomal malformation syndromes or as non-syndromic forms. Their etiology is not fully understood. Genetic variants such copy number variants have been associated with CHD. The aim of our study was to analyze these genomic variants in peripheral blood from Mexican children diagnosed with non-syndromic CHD. We included 16 children with atrial and ventricular septal defects and 5 healthy subjects without heart malformations as controls. To exclude the most common heart disease-associated syndrome alteration, we performed a fluorescence in situ hybridization test to identify the 22q11.2, responsible for congenital heart abnormalities associated with Di-George Syndrome. Then, a microarray based comparative genomic hybridization was used to identify global copy number variants. The identification of copy number variants resulted from the comparison and analysis between our results and data from main genetic variation databases. We identified copy number variants gain in three chromosomes regions from pediatric patients, 4q13.2 (31.25%), 9q34.3 (25%) and 20q13.33 (50%), where several genes associated with cellular, biosynthetic, and metabolic processes are located, UGT2B15, UGT2B17, SNAPC4, SDCCAG3, PMPCA, INPP6E, C9orf163, NOTCH1, C20orf166, and SLCO4A1. In addition, after a hierarchical cluster analysis based on the fluorescence intensity ratios from the comparative genomic hybridization, two congenital heart disease groups were generated corresponding to children with atrial or ventricular septal defects. Further analysis with a larger sample size is needed to corroborate these copy number variants as possible biomarkers to differentiate between heart abnormalities. Interestingly, the 20q13.33 gain was present in 50% of children with these CHD which could suggest that alterations in both coding and non-coding elements within this chromosomal region may play an important role in distinct heart conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aCGH" title="aCGH">aCGH</a>, <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title=" bioinformatics"> bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=congenital%20heart%20diseases" title=" congenital heart diseases"> congenital heart diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=copy%20number%20variants" title=" copy number variants"> copy number variants</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence%20in%20situ%20hybridization" title=" fluorescence in situ hybridization"> fluorescence in situ hybridization</a> </p> <a href="https://publications.waset.org/abstracts/53721/copy-number-variants-in-children-with-non-syndromic-congenital-heart-diseases-from-mexico" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53721.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">326</span> Numerical Studies for Standard Bi-Conjugate Gradient Stabilized Method and the Parallel Variants for Solving Linear Equations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kuniyoshi%20Abe">Kuniyoshi Abe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bi-conjugate gradient (Bi-CG) is a well-known method for solving linear equations Ax = b, for x, where A is a given n-by-n matrix, and b is a given n-vector. Typically, the dimension of the linear equation is high and the matrix is sparse. A number of hybrid Bi-CG methods such as conjugate gradient squared (CGS), Bi-CG stabilized (Bi-CGSTAB), BiCGStab2, and BiCGstab(l) have been developed to improve the convergence of Bi-CG. Bi-CGSTAB has been most often used for efficiently solving the linear equation, but we have seen the convergence behavior with a long stagnation phase. In such cases, it is important to have Bi-CG coefficients that are as accurate as possible, and the stabilization strategy, which stabilizes the computation of the Bi-CG coefficients, has been proposed. It may avoid stagnation and lead to faster computation. Motivated by a large number of processors in present petascale high-performance computing hardware, the scalability of Krylov subspace methods on parallel computers has recently become increasingly prominent. The main bottleneck for efficient parallelization is the inner products which require a global reduction. The resulting global synchronization phases cause communication overhead on parallel computers. The parallel variants of Krylov subspace methods reducing the number of global communication phases and hiding the communication latency have been proposed. However, the numerical stability, specifically, the convergence speed of the parallel variants of Bi-CGSTAB may become worse than that of the standard Bi-CGSTAB. In this paper, therefore, we compare the convergence speed between the standard Bi-CGSTAB and the parallel variants by numerical experiments and show that the convergence speed of the standard Bi-CGSTAB is faster than the parallel variants. Moreover, we propose the stabilization strategy for the parallel variants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bi-conjugate%20gradient%20stabilized%20method" title="bi-conjugate gradient stabilized method">bi-conjugate gradient stabilized method</a>, <a href="https://publications.waset.org/abstracts/search?q=convergence%20speed" title=" convergence speed"> convergence speed</a>, <a href="https://publications.waset.org/abstracts/search?q=Krylov%20subspace%20methods" title=" Krylov subspace methods"> Krylov subspace methods</a>, <a href="https://publications.waset.org/abstracts/search?q=linear%20equations" title=" linear equations"> linear equations</a>, <a href="https://publications.waset.org/abstracts/search?q=parallel%20variant" title=" parallel variant"> parallel variant</a> </p> <a href="https://publications.waset.org/abstracts/96373/numerical-studies-for-standard-bi-conjugate-gradient-stabilized-method-and-the-parallel-variants-for-solving-linear-equations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96373.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">164</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">325</span> Over Expression of Mapk8ip3 Patient Variants in Zebrafish to Establish a Spectrum of Phenotypes in a Rare-Neurodevelopmental Disorder</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kinnsley%20Travis">Kinnsley Travis</a>, <a href="https://publications.waset.org/abstracts/search?q=Camerron%20M.%20Crowder"> Camerron M. Crowder</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mapk8ip3 (Mitogen-Activated Protein Kinase 8 Interacting Protein 3) is a gene that codes for the JIP3 protein, which is a part of the JIP scaffolding protein family. This protein is involved in axonal vesicle transport, elongation and regeneration. Variants in the Mapk8ip3 gene are associated with a rare-genetic condition that results in a neurodevelopmental disorder that can cause a range of phenotypes including global developmental delay and intellectual disability. Currently, there are 18 known individuals diagnosed to have sequenced confirmed Mapk8ip3 genetic disorders. This project focuses on examining the impact of a subset of missense patient variants on the Jip3 protein function by overexpressing the mRNA of these variants in a zebrafish knockout model for Jip3. Plasmids containing cDNA with individual missense variants were reverse transcribed, purified, and injected into single-cell zebrafish embryos (Wild Type, Jip3 -/+, and Jip3 -/-). At 6-days post mRNA microinjection, morphological, behavioral, and microscopic phenotypes were examined in zebrafish larvae. Morphologically, we compared the size and shape of the zebrafish during their development over a 5-day period. Total locomotive activity was assessed using the Microtracker assay and patterns of movement over time were examined using the DanioVision assay. Lastly, we used confocal microscopy to examine sensory axons for swelling and shortened length, which are phenotypes observed in the loss-of-function knockout Jip3 zebrafish model. Using these assays during embryonic development, we determined the impact of various missense variants on Jip3 protein function, compared to knockout and wild-type zebrafish embryo models. Variants in the gene Mapk8ip3 cause rare-neurodevelopmental disorders due to an essential role in axonal vesicle transport, elongation and regeneration. A subset of missense variants was examined by overexpressing the mRNA of these variants in a Jip3 knock-out zebrafish. Morphological, behavioral, and microscopic phenotypes were examined in zebrafish larvae. Using these assays, the spectrum of disorders can be phenotypically determined and the impact of variant location can be compared to knockout and wild-type zebrafish embryo models. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rare%20disease" title="rare disease">rare disease</a>, <a href="https://publications.waset.org/abstracts/search?q=neurodevelopmental%20disorders" title=" neurodevelopmental disorders"> neurodevelopmental disorders</a>, <a href="https://publications.waset.org/abstracts/search?q=mrna%20overexpression" title=" mrna overexpression"> mrna overexpression</a>, <a href="https://publications.waset.org/abstracts/search?q=zebrafish%20research" title=" zebrafish research"> zebrafish research</a> </p> <a href="https://publications.waset.org/abstracts/154287/over-expression-of-mapk8ip3-patient-variants-in-zebrafish-to-establish-a-spectrum-of-phenotypes-in-a-rare-neurodevelopmental-disorder" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/154287.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">116</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">324</span> Pharmacokinetic Model of Warfarin and Its Application in Personalized Medicine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vijay%20Kumar%20Kutala">Vijay Kumar Kutala</a>, <a href="https://publications.waset.org/abstracts/search?q=Addepalli%20Pavani"> Addepalli Pavani</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Amresh%20Rao"> M. Amresh Rao</a>, <a href="https://publications.waset.org/abstracts/search?q=Naushad%20Sm"> Naushad Sm</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we evaluated the impact of CYP2C9*2 and CYP2C9*3 variants on binding and hydroxylation of warfarin. In silico data revealed that warfarin forms two hydrogen bonds with protein backbone i.e. I205 and S209, one hydrogen bond with protein side chain i.e. T301 and stacking interaction with F100 in CYP2C9*1. In CYP2C9*2 and CYP2C9*3 variants, two hydrogen bonds with protein backbone are disrupted. In double variant, all the hydrogen bonds are disrupted. The distances between C7 of S-warfarin and Fe-O in CYP2C9*1, CYP2C9*2, CYP2C9*3 and CYP2C9*2/*3 were 5.81A°, 7.02A°, 7.43° and 10.07°, respectively. The glide scores (Kcal/mol) were -7.698, -7.380, -6.821 and -6.986, respectively. Increase in warfarin/7-hydroxy warfarin ratio was observed with increase in variant alleles. To conclude, CYP2C9*2 and CYP2C9*3 variants result in disruption of hydrogen bonding interactions with warfarin and longer distance between C7 and Fe-O thus impairing warfarin 7-hydroxylation due to lower binding affinity of warfarin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=warfarin" title="warfarin">warfarin</a>, <a href="https://publications.waset.org/abstracts/search?q=CYP2C9%20polymorphism" title=" CYP2C9 polymorphism"> CYP2C9 polymorphism</a>, <a href="https://publications.waset.org/abstracts/search?q=personalized%20medicine" title=" personalized medicine"> personalized medicine</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20Silico" title=" in Silico"> in Silico</a> </p> <a href="https://publications.waset.org/abstracts/24956/pharmacokinetic-model-of-warfarin-and-its-application-in-personalized-medicine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24956.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">322</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">323</span> Genetic Association and Functional Significance of Matrix Metalloproteinase-14 Promoter Variants rs1004030 and rs1003349 in Gallbladder Cancer Pathogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=J.%20Vinay">J. Vinay </a>, <a href="https://publications.waset.org/abstracts/search?q=Kusumbati%20Besra"> Kusumbati Besra</a>, <a href="https://publications.waset.org/abstracts/search?q=Niharika%20Pattnaik"> Niharika Pattnaik</a>, <a href="https://publications.waset.org/abstracts/search?q=Shivaram%20Prasad%20Singh"> Shivaram Prasad Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Manjusha%20Dixit"> Manjusha Dixit </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gallbladder cancer (GBC) is rare but highly malignant cancer; its prevalence is more in certain geographical regions and ethnic groups, which include the Northern and Eastern states of India. Previous studies in India have reported genetic predisposition as one of the risk factors in GBC pathogenesis. Although the matrix metalloproteinase-14 (MMP14) is a well-known modulator of the tumor microenvironment and tumorigenesis and TCGA data also suggests its upregulation yet, its role in the genetic predisposition for GBC is completely unknown. We elucidated the role of MMP14 promoter variants as genetic risk factors and their implications in expression modulation. We screened MMP14 promoter variants association with GBC using Sanger’s sequencing in approximately 300 GBC and 300 control subjects and 26 GBC tissue samples of Indian ethnicity. The immunohistochemistry was used to check the MMP14 protein expression in GBC tissue samples. The role of promoter variants on expression levels was elucidated using a luciferase reporter assay. The variants rs1004030 (p-value = 0.0001) and rs1003349 (p-value = 0.0008) were significantly associated with gallbladder cancer. The luciferase assay in two different cell lines, HEK-293 (p = 0.0006) and TGBC1TKB (p = 0.0036) showed a significant increase in relative luciferase activity in the presence of risk alleles for both the single nucleotide polymorphisms (SNPs). Similarly, genotype-phenotype correlation in patients samples confirmed that the presence of risk alleles at rs1004030 and rs1003349 increased MMP14 expression. Overall, this study unravels the genetic association of MMP14 promoter variants with gallbladder cancer, which may contribute to pathogenesis by increasing its expression. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gallbladder%20cancer" title="gallbladder cancer">gallbladder cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix%20metalloproteinase-14" title=" matrix metalloproteinase-14"> matrix metalloproteinase-14</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20nucleotide%20polymorphism" title=" single nucleotide polymorphism"> single nucleotide polymorphism</a>, <a href="https://publications.waset.org/abstracts/search?q=case%20control%20study" title=" case control study"> case control study</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20association%20study" title=" genetic association study"> genetic association study</a> </p> <a href="https://publications.waset.org/abstracts/134568/genetic-association-and-functional-significance-of-matrix-metalloproteinase-14-promoter-variants-rs1004030-and-rs1003349-in-gallbladder-cancer-pathogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/134568.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">179</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">322</span> Whole Coding Genome Inter-Clade Comparisons to Predict Global Cancer-Protecting Variants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lamis%20Naddaf">Lamis Naddaf</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuval%20Tabach"> Yuval Tabach</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We identified missense genetic variants with the potential to enhance resistance against cancer. Such a field has not been widely explored as researchers tend to investigate the mutations that cause diseases, in response to the suffering of patients, rather than those mutations that protect from them. In conjunction with the genomic revolution and the advances in genetic engineering and synthetic biology, identifying the protective variants will increase the power of genotype-phenotype predictions and have significant implications for improved risk estimation, diagnostics, prognosis, and even personalized therapy and drug discovery. To approach our goal, we systematically investigated the sites of the coding genomes and selected the alleles that showed a correlation with the species’ cancer resistance. Interestingly, we found several amino acids that are more generally preferred (like the Proline) or avoided (like the Cysteine) by the resistant species. Furthermore, Cancer resistance in mammals and reptiles is significantly predicted by the number of the predicted protecting variants (PVs) a species has. Moreover, PVs-enriched-genes are enriched in pathways relevant to tumor suppression. For example, they are enriched in the Hedgehog signaling and silencing pathways, which its improper activation is associated with the most common form of cancer malignancy. We also showed that the PVs are mostly more abundant in healthy people compared to cancer patients within different human races. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cancer%20resistance" title="cancer resistance">cancer resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=protecting%20variant" title=" protecting variant"> protecting variant</a>, <a href="https://publications.waset.org/abstracts/search?q=naked%20mole%20rat" title=" naked mole rat"> naked mole rat</a>, <a href="https://publications.waset.org/abstracts/search?q=comparative%20genomics" title=" comparative genomics"> comparative genomics</a> </p> <a href="https://publications.waset.org/abstracts/158145/whole-coding-genome-inter-clade-comparisons-to-predict-global-cancer-protecting-variants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/158145.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">111</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">321</span> Genetic Determinants of Ovarian Response to Gonadotropin Stimulation in Women Undergoing Assisted Reproductive Treatment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20Tohlob">D. Tohlob</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Abo%20Hashem"> E. Abo Hashem</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Ghareeb"> N. Ghareeb</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Ghanem"> M. Ghanem</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Elfarahaty"> R. Elfarahaty</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20A.%20Roberts"> S. A. Roberts</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Pemberton"> P. Pemberton</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Mohiyiddeen"> L. Mohiyiddeen</a>, <a href="https://publications.waset.org/abstracts/search?q=W.%20G.%20Newman"> W. G. Newman </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gonadotropin stimulation is used in females undergoing assisted reproductive treatment for ovulation induction, but ovarian response is variable and unpredictable in these women. More effective protocols and individualization of treatment are needed to increase the success rate of IVF/ICSI cycles. We genotyped seven variants reported in previous studies to be associated with ovarian response (number of ova retrieved and total gonadotropin dose) in women undergoing IVF treatment including FSHR variants Asn 680 Ser (c.2039 A > G), Thr 307 Ala (c. 919 > A), -29 G > A, HRG c.610 C > T gene, BMP15 -9 C > G, AMH Ile 49 Ser (c.146 G > T), and AMHR -489A˃G in 118 Egyptian females attending Mansoura Integrated Fertility Center in Egypt, these females were undergoing their first cycle of controlled ovarian hyper stimulation for IVF/ICSI treatment. They were analyzed by TaqMan allelic discrimination assay in Manchester Center of Genomic Medicine. We found no evidence of any significant difference (p value < 0.05) in the number of eggs retrieved or the gonadotropin dose used between individuals in all genotypes except for HRG c.610 C > T gene polymorphism where regression analysis gives a p value of 0.04 with a fewer eggs number in TT genotyped females. These results indicate that these variants do not provide sufficient clinically relevant data to individualize the treatment protocols. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=controlled%20ovarian%20hyperstimulation" title="controlled ovarian hyperstimulation">controlled ovarian hyperstimulation</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20variants" title=" gene variants"> gene variants</a>, <a href="https://publications.waset.org/abstracts/search?q=ovarian%20response" title=" ovarian response"> ovarian response</a>, <a href="https://publications.waset.org/abstracts/search?q=assisted%20reproduction" title=" assisted reproduction"> assisted reproduction</a> </p> <a href="https://publications.waset.org/abstracts/37258/genetic-determinants-of-ovarian-response-to-gonadotropin-stimulation-in-women-undergoing-assisted-reproductive-treatment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37258.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">319</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">320</span> Association of Nuclear – Mitochondrial Epistasis with BMI in Type 1 Diabetes Mellitus Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Agnieszka%20H.%20Ludwig-Slomczynska">Agnieszka H. Ludwig-Slomczynska</a>, <a href="https://publications.waset.org/abstracts/search?q=Michal%20T.%20Seweryn"> Michal T. Seweryn</a>, <a href="https://publications.waset.org/abstracts/search?q=Przemyslaw%20Kapusta"> Przemyslaw Kapusta</a>, <a href="https://publications.waset.org/abstracts/search?q=Ewelina%20Pitera"> Ewelina Pitera</a>, <a href="https://publications.waset.org/abstracts/search?q=Katarzyna%20Cyganek"> Katarzyna Cyganek</a>, <a href="https://publications.waset.org/abstracts/search?q=Urszula%20Mantaj"> Urszula Mantaj</a>, <a href="https://publications.waset.org/abstracts/search?q=Lucja%20Dobrucka"> Lucja Dobrucka</a>, <a href="https://publications.waset.org/abstracts/search?q=Ewa%20Wender-Ozegowska"> Ewa Wender-Ozegowska</a>, <a href="https://publications.waset.org/abstracts/search?q=Maciej%20T.%20Malecki"> Maciej T. Malecki</a>, <a href="https://publications.waset.org/abstracts/search?q=Pawel%20Wolkow"> Pawel Wolkow</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Obesity results from an imbalance between energy intake and its expenditure. Genome-Wide Association Study (GWAS) analyses have led to discovery of only about 100 variants influencing body mass index (BMI), which explain only a small portion of genetic variability. Analysis of gene epistasis gives a chance to discover another part. Since it was shown that interaction and communication between nuclear and mitochondrial genome are indispensable for normal cell function, we have looked for epistatic interactions between the two genomes to find their correlation with BMI. Methods: The analysis was performed on 366 T1DM patients using Illumina Infinium OmniExpressExome-8 chip and followed by imputation on Michigan Imputation Server. Only genes which influence mitochondrial functioning (listed in Human MitoCarta 2.0) were included in the analysis – variants of nuclear origin (MAF > 5%) in 1140 genes and 42 mitochondrial variants (MAF > 1%). Gene expression analysis was performed on GTex data. Association analysis between genetic variants and BMI was performed with the use of Linear Mixed Models as implemented in the package 'GENESIS' in R. Analysis of association between mRNA expression and BMI was performed with the use of linear models and standard significance tests in R. Results: Among variants involved in epistasis between mitochondria and nucleus we have identified one in mitochondrial transcription factor, TFB2M (rs6701836). It interacted with mitochondrial variants localized to MT-RNR1 (p=0.0004, MAF=15%), MT-ND2 (p=0.07, MAF=5%) and MT-ND4 (p=0.01, MAF=1.1%). Analysis of the interaction between nuclear variant rs6701836 (nuc) and rs3021088 localized to MT-ND2 mitochondrial gene (mito) has shown that the combination of the two led to BMI decrease (p=0.024). Each of the variants on its own does not correlate with higher BMI [p(nuc)=0.856, p(mito)=0.116)]. Although rs6701836 is intronic, it influences gene expression in the thyroid (p=0.000037). rs3021088 is a missense variant that leads to alanine to threonine substitution in the MT-ND2 gene which belongs to complex I of the electron transport chain. The analysis of the influence of genetic variants on gene expression has confirmed the trend explained above – the interaction of the two genes leads to BMI decrease (p=0.0308). Each of the mRNAs on its own is associated with higher BMI (p(mito)=0.0244 and p(nuc)=0.0269). Conclusıons: Our results show that nuclear-mitochondrial epistasis can influence BMI in T1DM patients. The correlation between transcription factor expression and mitochondrial genetic variants will be subject to further analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=body%20mass%20index" title="body mass index">body mass index</a>, <a href="https://publications.waset.org/abstracts/search?q=epistasis" title=" epistasis"> epistasis</a>, <a href="https://publications.waset.org/abstracts/search?q=mitochondria" title=" mitochondria"> mitochondria</a>, <a href="https://publications.waset.org/abstracts/search?q=type%201%20diabetes" title=" type 1 diabetes"> type 1 diabetes</a> </p> <a href="https://publications.waset.org/abstracts/90791/association-of-nuclear-mitochondrial-epistasis-with-bmi-in-type-1-diabetes-mellitus-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90791.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">175</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">319</span> Variants of Mathematical Induction as Strong Proof Techniques in Theory of Computing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Tarek">Ahmed Tarek</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Alveed"> Ahmed Alveed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the theory of computing, there are a wide variety of direct and indirect proof techniques. However, mathematical induction (MI) stands out to be one of the most powerful proof techniques for proving hypotheses, theorems, and new results. There are variations of mathematical induction-based proof techniques, which are broadly classified into three categories, such as structural induction (SI), weak induction (WI), and strong induction (SI). In this expository paper, several different variants of the mathematical induction techniques are explored, and the specific scenarios are discussed where a specific induction technique stands out to be more advantageous as compared to other induction strategies. Also, the essential difference among the variants of mathematical induction are explored. The points of separation among mathematical induction, recursion, and logical deduction are precisely analyzed, and the relationship among variations of recurrence relations, and mathematical induction are being explored. In this context, the application of recurrence relations, and mathematical inductions are considered together in a single framework for codewords over a given alphabet. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alphabet" title="alphabet">alphabet</a>, <a href="https://publications.waset.org/abstracts/search?q=codeword" title=" codeword"> codeword</a>, <a href="https://publications.waset.org/abstracts/search?q=deduction" title=" deduction"> deduction</a>, <a href="https://publications.waset.org/abstracts/search?q=mathematical" title=" mathematical"> mathematical</a>, <a href="https://publications.waset.org/abstracts/search?q=induction" title=" induction"> induction</a>, <a href="https://publications.waset.org/abstracts/search?q=recurrence%20relation" title=" recurrence relation"> recurrence relation</a>, <a href="https://publications.waset.org/abstracts/search?q=strong%20induction" title=" strong induction"> strong induction</a>, <a href="https://publications.waset.org/abstracts/search?q=structural%20induction" title=" structural induction"> structural induction</a>, <a href="https://publications.waset.org/abstracts/search?q=weak%20induction" title=" weak induction"> weak induction</a> </p> <a href="https://publications.waset.org/abstracts/116467/variants-of-mathematical-induction-as-strong-proof-techniques-in-theory-of-computing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116467.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">164</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">318</span> Determination of the CCR5Δ32 Frequency in Emiratis and Tunisians and Screening of the CCR5 Gene for Novel Alleles in Emiratis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20A.%20Al-Jaberi">Sara A. Al-Jaberi</a>, <a href="https://publications.waset.org/abstracts/search?q=Salma%20Ben-Salem"> Salma Ben-Salem</a>, <a href="https://publications.waset.org/abstracts/search?q=Meriam%20Messedi"> Meriam Messedi</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatma%20Ayadi"> Fatma Ayadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Lihadh%20Al-Gazali"> Lihadh Al-Gazali</a>, <a href="https://publications.waset.org/abstracts/search?q=Bassam%20R.%20Ali"> Bassam R. Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The chemokine receptor components play crucial roles in the immune system and some of them serve as co-receptors for the HIV virus. Several studies have documented those variants in chemokine receptors are correlated with susceptibility and resistance to infection with HIV virus. For example, mutations in the chemokine receptor 5 gene (CCR5) resulting in loss-of-function (such as the homozygous CCR5Δ32) confer high degree of resistance to HIV infection. Heterozygotes for these variants exhibit slow progression to AIDS. The prevalence of CCR5 polymorphisms varies among ethnic and geographical groups. For example, the CCR5 Δ32 variant is present in 10–15% of north Europeans but is rarely encountered among Africans. This study aims to identify the prevalence of some CCR5 variants in two geographically distant Arab populations (namely Emiratis and Tunisians). Methodology: The prevalence of CCR5 gene variants including CCR5Δ32, FS299, C101X, A29S and C178R has been determined using PCR and direct DNA sequencing. A total of 403 unrelated healthy individuals (253 Emiratis and 150 Tunisians) were genotyped for the CCR5Δ32 variant using PCR amplification and gel electrophoresis. In addition, 200 Emiratis have been screened for other SNPs using Sanger DNA sequencing. Results: Among Emiratis, the allele frequency of the CCR5Δ32 variant has been found to be 0.002. In addition, two variants L55Q and A159 were found at a frequency of 0.002.Moreover, the prevalence of the CCR5Δ32 variant in Tunisians was estimated to be 0.013 which is relatively higher than its frequency in Emiratis but lower than Europeans. Conclusion: We conclude that the allele frequency of the most critical CCR5 polymorphism (Δ32) is extremely low among Emiratis compared to other Arabs and North Europeans. In addition, very low allele frequencies of other CCR5 polymorphisms have been detected among Emiratis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chemokine%20receptors" title="chemokine receptors">chemokine receptors</a>, <a href="https://publications.waset.org/abstracts/search?q=CCR5%CE%9432" title=" CCR5Δ32"> CCR5Δ32</a>, <a href="https://publications.waset.org/abstracts/search?q=CCR5%20polymorphisms" title=" CCR5 polymorphisms"> CCR5 polymorphisms</a>, <a href="https://publications.waset.org/abstracts/search?q=Emiratis" title=" Emiratis"> Emiratis</a>, <a href="https://publications.waset.org/abstracts/search?q=Arab%20populations" title=" Arab populations"> Arab populations</a> </p> <a href="https://publications.waset.org/abstracts/5778/determination-of-the-ccr5d32-frequency-in-emiratis-and-tunisians-and-screening-of-the-ccr5-gene-for-novel-alleles-in-emiratis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5778.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">378</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">317</span> Genetic Diversity and Discovery of Unique SNPs in Five Country Cultivars of Sesamum indicum by Next-Generation Sequencing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nam-Kuk%20Kim">Nam-Kuk Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Jin%20Kim"> Jin Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Soomin%20Park"> Soomin Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Changhee%20Lee"> Changhee Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Mijin%20Chu"> Mijin Chu</a>, <a href="https://publications.waset.org/abstracts/search?q=Seong-Hun%20Lee"> Seong-Hun Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we conducted whole genome re-sequencing of 10 cultivars originated from five countries including Korea, China, India, Pakistan and Ethiopia with Sesamum indicum (Zhongzho No. 13) genome as a reference. Almost 80% of the whole genome sequences of the reference genome could be covered by sequenced reads. Numerous SNP and InDel were detected by bioinformatic analysis. Among these variants, 266,051 SNPs were identified as unique to countries. Pakistan and Ethiopia had high densities of SNPs compared to other countries. Three main clusters (cluster 1: Korea, cluster 2: Pakistan and India, cluster 3: Ethiopia and China) were recovered by neighbor-joining analysis using all variants. Interestingly, some variants were detected in DGAT1 (diacylglycerol O-acyltransferase 1) and FADS (fatty acid desaturase) genes, which are known to be related with fatty acid synthesis and metabolism. These results can provide useful information to understand the regional characteristics and develop DNA markers for origin discrimination of sesame. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sesamum%20indicum" title="Sesamum indicum">Sesamum indicum</a>, <a href="https://publications.waset.org/abstracts/search?q=NGS" title=" NGS"> NGS</a>, <a href="https://publications.waset.org/abstracts/search?q=SNP" title=" SNP"> SNP</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20marker" title=" DNA marker"> DNA marker</a> </p> <a href="https://publications.waset.org/abstracts/54776/genetic-diversity-and-discovery-of-unique-snps-in-five-country-cultivars-of-sesamum-indicum-by-next-generation-sequencing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54776.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">327</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">316</span> Functional Variants Detection by RNAseq</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Raffaele%20A.%20Calogero">Raffaele A. Calogero</a> </p> <p class="card-text"><strong>Abstract:</strong></p> RNAseq represents an attractive methodology for the detection of functional genomic variants. RNAseq results obtained from polyA+ RNA selection protocol (POLYA) and from exonic regions capturing protocol (ACCESS) indicate that ACCESS detects 10% more coding SNV/INDELs with respect to POLYA. ACCESS requires less reads for coding SNV detection with respect to POLYA. However, if the analysis aims at identifying SNV/INDELs also in the 5’ and 3’ UTRs, POLYA is definitively the preferred method. No particular advantage comes from ACCESS or POLYA in the detection of fusion transcripts. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fusion%20transcripts" title="fusion transcripts">fusion transcripts</a>, <a href="https://publications.waset.org/abstracts/search?q=INDEL" title=" INDEL"> INDEL</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA-seq" title=" RNA-seq"> RNA-seq</a>, <a href="https://publications.waset.org/abstracts/search?q=WES" title=" WES"> WES</a>, <a href="https://publications.waset.org/abstracts/search?q=SNV" title=" SNV"> SNV</a> </p> <a href="https://publications.waset.org/abstracts/57993/functional-variants-detection-by-rnaseq" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57993.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">287</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">315</span> Alternative Splicing of an Arabidopsis Gene, At2g24600, Encoding Ankyrin-Repeat Protein</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20Sakamoto">H. Sakamoto</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Kurosawa"> S. Kurosawa</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Suzuki"> M. Suzuki</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Oguri"> S. Oguri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In Arabidopsis, several genes encoding proteins with ankyrin repeats and trans-membrane domains (AtANKTM) have been identified as mediators of biotic and abiotic stress responses. It has been known that the expression of an AtANKTM gene, At2g24600, is induced in response to abiotic stress and that there are four splicing variants derived from this locus. In this study, by RT-PCR and sequencing analysis, an unknown splicing variant of the At2g24600 transcript was identified. Based on differences in the predicted amino acid sequences, the five splicing variants are divided into three groups. The three predicted proteins are highly homologous, yet have different numbers of ankyrin repeats and trans-membrane domains. It is generally considered that ankyrin repeats mediate protein-protein interaction and that the number of trans-membrane domains affects membrane topology of proteins. The protein variants derived from the At2g24600 locus may have different molecular functions each other. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alternative%20splicing" title="alternative splicing">alternative splicing</a>, <a href="https://publications.waset.org/abstracts/search?q=ankyrin%20repeats" title=" ankyrin repeats"> ankyrin repeats</a>, <a href="https://publications.waset.org/abstracts/search?q=trans-membrane%20domains" title=" trans-membrane domains"> trans-membrane domains</a>, <a href="https://publications.waset.org/abstracts/search?q=arabidopsis" title=" arabidopsis"> arabidopsis</a> </p> <a href="https://publications.waset.org/abstracts/6374/alternative-splicing-of-an-arabidopsis-gene-at2g24600-encoding-ankyrin-repeat-protein" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/6374.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">374</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">314</span> An Approach to Make an Adaptive Immunoassay to Detect an Unknown Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Josselyn%20Mata%20Calidonio">Josselyn Mata Calidonio</a>, <a href="https://publications.waset.org/abstracts/search?q=Arianna%20I.%20Maddox"> Arianna I. Maddox</a>, <a href="https://publications.waset.org/abstracts/search?q=Kimberly%20Hamad-Schifferli"> Kimberly Hamad-Schifferli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapid diagnostics are critical infectious disease tools that are designed to detect a known biomarker using antibodies specific to that biomarker. However, a way to detect unknown viruses has not yet been achieved in a paper test format. We describe here a route to make an adaptable paper immunoassay that can detect an unknown biomarker, demonstrating it on SARS-CoV-2 variants. The immunoassay repurposes cross-reactive antibodies raised against the alpha variant. Gold nanoparticles of two different colors conjugated to two different antibodies create a colorimetric signal, and machine learning of the resulting colorimetric pattern is used to train the assay to discriminate between variants of alpha and Omicron BA.5. By using principal component analysis, the colorimetric test patterns can pick up and discriminate an unknown that it has not encountered before, Omicron BA.1. The test has an accuracy of 100% and a potential calculated discriminatory power of 900. We show that it can be used adaptively and that it can be used to pick up emerging variants without the need to raise new antibodies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adaptive%20immunoassay" title="adaptive immunoassay">adaptive immunoassay</a>, <a href="https://publications.waset.org/abstracts/search?q=detecting%20unknown%20viruses" title=" detecting unknown viruses"> detecting unknown viruses</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20nanoparticles" title=" gold nanoparticles"> gold nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=paper%20immunoassay" title=" paper immunoassay"> paper immunoassay</a>, <a href="https://publications.waset.org/abstracts/search?q=repurposing%20antibodies" title=" repurposing antibodies"> repurposing antibodies</a> </p> <a 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