Recombinant Human His6-USP10 Protein, CF (E-592) by R&D Systems, Part of Bio-Techne

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data-time_datapoints="-0.090286016464233" data-time_template="0.090286016464233" data-time_post_epcc="0.52575206756592" data-time_epcc="-0.090286016464233" data-render_time="Friday 22nd of November 2024 09:53:30 AM" data-fallback="FALSE" data-time_to_process_data="0.081122159957886" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Recombinant Human His6-USP10 Protein, CF </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">E-592</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="reviews_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="reviews_quicklink"> <a href="#reviews" 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Enzymes" data-caption="" data-badges='' data-id="0" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="alert_content" class=".alert .alert-success" > <div class="alert alert-success p-2" role="alert"> <div class="d-inline-flex p-1"> <div class="pr-2"> <span class="icon-note-important"></span> </div> <div> <p>Please inquire regarding future lot manufacture.</p> </div> </div> </div> </div> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="E-592"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="E-592-050" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">E-592-050</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> 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class="tab-content col-xl-9 mt-2"> <div data-section="section-base--nav-tabs-content"> <ul class="nav nav-tabs nav-fill" id="productTab" role="tablist"> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor active" href="#tab-technical_data" id="technical_data-tab" data-toggle="tab" data-target="#technical_data" type="button" role="tab" aria-controls="technical_data" aria-selected="true"> <p class="m-0">Technical Data</p> </a> </li> <li class="nav-item" role="presentation"> <a class="nav-link tab-anchor " href="#tab-citations_reviews" id="citations_reviews-tab" data-toggle="tab" data-target="#citations_reviews" type="button" role="tab" aria-controls="citations_reviews" aria-selected="false"> <p class="m-0">Citations & Reviews</p> </a> </li> </ul> <div id="ProductTabContent" class="tab-content mb-3"> <div class="tab-pane fade show active" id="technical_data" role="tabpanel" aria-labelledby="technical_data-tab"> <div data-section="section-base--technical-anchors"> <ul class="product-pages-anchors nav mt-2"> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor active" href="#product-specifications"> Product Specifications</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#preparation--storage"> Preparation & Storage</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#background"> Background</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#product-documents"> Product Documents</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#specific-notices"> Specific Notices</a> </li> </ul> </div> <div id="key_details" class="py-5 bt-pp-border-bottom" data-section="section-base--key-details"> <h3>Key Product Details</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Sf 21 (baculovirus) </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Accession #</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <a href="https://www.ncbi.nlm.nih.gov/protein/Q14694.2" class="a___link-external" target="_blank" rel="nofollow">Q14694.2</a> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Conjugate</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Unconjugated </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Bioactivity </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/usp10/proteins-enzymes">View all USP10 Proteins and Enzymes »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="E-592" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <em>Spodoptera frugiperda</em>, <em>Sf</em> 21 (baculovirus)-derived human USP10 protein<br>Met1 - Leu798 with a C-terminal 6-His tag </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Predicted Molecular Mass</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 89 kDa </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Activity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <p>Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-USP10 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates ((Catalog # <a class="NoLineLink" href="/p/proteins-enzymes/recombinant-human-ubiquitin-amc-protein-cf_u-550" target="_self">U-550</a> or <a class="NoLineLink" href="/p/proteins-enzymes/recombinant-human-ubiquitin-rhodamine-110-protein-cf_u-555" target="_self">U-555</a>)).</p> </div> </div> </div> <h4 class="mt-3">Reviewed Applications</h4> <p><span class="review_stars review_stars_5"></span> Read <a href="#reviews" class="reviews">1 review</a> rated 5 using E-592 in the following applications:</p> <ul class="list-unstyled"> <li><a href="#reviews" class="reviews" data-application="Enzymatic activity in vitro">Enzymatic activity in vitro (1 Review) </a></li> </ul> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-protein--preparation-storage"> <h3>Formulation, Preparation and Storage</h3> <div class="datasheet_section"> <div class="row px-3"> <div class="col-xs-12 "> <div class="border-left border-right border-top text-center"> <h5 class="m-0 p-3 bg-dark-bt-blue text-white"> E-592 </h5> </div> <table class="ds_table_html table-bordered tablesaw tablesaw-stack"> <tbody> <tr> <td class="font-weight-bold">Formulation</td> <td class="w-75">Supplied as a solution in HEPES, NaCl, TCEP, and Glycerol.</td> </tr> <tr> <td class="font-weight-bold">Shipping</td> <td class="w-75">The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.</td> </tr> <tr> <td class="font-weight-bold">Stability & Storage</td> <td class="w-75"><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>6 months from date of receipt, -70 °C as supplied.</LI><LI>3 months, -70 °C under sterile conditions after opening.</LI></UL></td> </tr> </tbody> </table> </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: USP10</h3> <article class="product-data-wrapper"> <P>Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a specialized cysteine protease with a predicted molecular weight of 87 kDa. USP10 is a member of the peptidase C19 family and the human protein shares 83% amino acid sequence identity with its mouse ortholog. Reported USP10 targets include p53, BECN1, SNX3 and CFTR. USP10 translocates to the nucleus in response to DNA damage and deubiquitinates p53, thus regulating the p53-dependent DNA damage response. USP10 deubiquitination of BECN1 leads to stabilization of PIK3C3/VPS34-containing complexes which drives autophagy. The small molecule Spautin-1 exerts its potent inhibitory effects on autophagy partially through the inhibition of USP10 activity.</P> </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>References</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <ol> <li>Boulkroun S., <em>et al.</em> (2008) Am. J. Physiol. <strong>295</strong>:F889.</li> <li>Liu J., <em>et al.</em> (2011) Cell <strong>147</strong>: 223.</li> <li>Yuan J., <em>et al.</em> (2010) Cell <strong>140</strong>: 384.</li></ol> </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Ubiquitin Specific Protease 10 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Ubiquitin Thiolesterase 10, UBPO, Uchrp </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Entrez Gene IDs</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.ncbi.nlm.nih.gov/gene?term=9100" class="a___link-external" target="_blank" rel="nofollow">9100</a> (Human); <a href="https://www.ncbi.nlm.nih.gov/gene?term=22224" class="a___link-external" target="_blank" rel="nofollow">22224</a> (Mouse); <a href="https://www.ncbi.nlm.nih.gov/gene?term=307905" class="a___link-external" target="_blank" rel="nofollow">307905</a> (Rat) </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Gene Symbol</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> USP10 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>UniProt</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.uniprot.org/uniprot/?query=Q14694.2" class="a___link-external" target="_blank" rel="nofollow">Q14694.2</a> </div> </div> </div> <h4 class="mt-3">Additional USP10 Products</h4> <ul class="list-unstyled"> <li><a href="/t/usp10" data-product="USP10">All Products for USP10 </a></li> <li><a href="/t/usp10/lysates" data-application="USP10 Lysates">USP10 Lysates </a></li> <li><a href="/t/usp10/antibodies" data-application="USP10 Primary Antibodies">USP10 Primary Antibodies </a></li> <li><a href="/t/usp10/proteins-enzymes" data-application="USP10 Proteins and Enzymes">USP10 Proteins and Enzymes </a></li> </ul> </div> <div id="product-documents" data-section="section-base--product-documents"> <div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3"> Product Documents for Recombinant Human His6-USP10 Protein, CF </h3> </div> <div class="vtab-section" data-section="Product Datasheets"> <div class="card-header rounded-top"> <a class="" data-toggle="collapse" data-target="#collapse_two" aria-expanded="true" aria-controls="collapse_two" id="product-datasheets-link"> <h5 class="mb-0"> Product Datasheets </h5> </a> </div> <div id="collapse_two" class="collapse show prod_datasheet_link" aria-labelledby="heading_two" data-parent="#accordion"> <div class="card-body"> <div class="row"> <div class="col-md-6"> <a href="https://resources.rndsystems.com/pdfs/datasheets/e-592.pdf" class="datasheet_link" target="_blank" rel="nofollow">Download Product Datasheets</a> </div> </div> </div> </div> </div> <div class="card-header"> <a data-toggle="collapse" data-target="#collapse_one" aria-expanded="false" class="collapsed" aria-controls="collapse_one"> <h5 class="mb-0"> COA </h5> </a> </div> <div id="collapse_one" class="collapse " aria-labelledby="heading_one" data-parent="#accordion"> <div class="card-body"> <form novalidate="novalidate" data-drupal-selector="product-coa-form" action="#coa-form" method="post" id="product-coa-form" accept-charset="UTF-8"> <div class="row"> <div class="col-12"> <h2>Certificate of Analysis</h2> <p>To download a Certificate of Analysis, please enter a lot number in the search box below.</p> <p><strong>Note:</strong> Certificate of Analysis not available for kit components.</p> </div> </div> <section class="row"> <div class="col-md-6"> <form novalidate="novalidate" data-drupal-selector="product-coa-form"> <div class="form-row align-items-center"> <div class="col-md-10 my-1"> <input data-drupal-selector="form-znepkz3dsxghavhp-tftiqt-bvebgwc5x2gljuewxxk" type="hidden" name="form_build_id" value="form-zNEpKz3dsxghAVhP-tftiQT-BveBgWc5X2GLJUEWXXk" /> <input data-drupal-selector="edit-product-coa-form" type="hidden" name="form_id" value="product_coa_form" /> <input data-drupal-selector="edit-product-code" type="hidden" name="product_code" value="E-592" /> <div class="js-form-item form-item pb-3 js-form-type-textfield form-item-lot-number js-form-item-lot-number form-no-label"> <input autocomplete="off" class="form-control cofa-lookup form-text" id="lot-number" placeholder="Search by lot number" data-drupal-selector="edit-lot-number" type="text" name="lot_number" value="" 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Recombinant Human His6-USP10 Protein, CF (E-592) by R&D Systems, Part of Bio-Techne