Search results for: Yoshihisa Hagihara

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Hagihara</title> <meta name="description" content="Search results for: Yoshihisa Hagihara"> <meta name="keywords" content="Yoshihisa Hagihara"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Yoshihisa Hagihara"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Yoshihisa Hagihara</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Introduction of PMMA-Tag to VHH for Improving Recovery and Immobilization Rate of VHHS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bongmun%20Kang">Bongmun Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=Kagnari%20Yamakawa"> Kagnari Yamakawa</a>, <a href="https://publications.waset.org/abstracts/search?q=Yoshihisa%20Hagihara"> Yoshihisa Hagihara</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuji%20Ito"> Yuji Ito</a>, <a href="https://publications.waset.org/abstracts/search?q=Michimasa%20Kishimoto"> Michimasa Kishimoto</a>, <a href="https://publications.waset.org/abstracts/search?q=Yoichi%20Kumada"> Yoichi Kumada</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The PMMA-tag was genetically fused with the C-terminal region of VHH molecules. This antibody, VHH, is known as a single-chain domain, which is devoid of light chains. The PMMA-tag, which could affect the isoelectric point (pI) changeable with a charge of amino acid in VHHs were closely related to the solubility of VHH molecules during refolding. The genetic fusion of PMMA-tag to C-terminal region of VHHs significantly affects the recovery of their soluble protein during refolding by 50 mM TAPS at pH 8.5. It could be refolded with a recovery of more than 95% by dialysis at pH 8.5. A marked difference in the antigen-binding activities in the adsorption state was significantly high in VHH-PM compared to the wild type of VHH. There are approximately 8-fold differences in the antigen-binding activities in the adsorption state between VHH-PM and VHH. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=VHH" title="VHH">VHH</a>, <a href="https://publications.waset.org/abstracts/search?q=PMMA-tag" title=" PMMA-tag"> PMMA-tag</a>, <a href="https://publications.waset.org/abstracts/search?q=isoelectric%20point" title=" isoelectric point"> isoelectric point</a>, <a href="https://publications.waset.org/abstracts/search?q=pH" title=" pH"> pH</a>, <a href="https://publications.waset.org/abstracts/search?q=Solubility" title=" Solubility"> Solubility</a>, <a href="https://publications.waset.org/abstracts/search?q=refolding" title=" refolding"> refolding</a>, <a href="https://publications.waset.org/abstracts/search?q=immobilization" title=" immobilization"> immobilization</a>, <a href="https://publications.waset.org/abstracts/search?q=ELISA" title=" ELISA"> ELISA</a> </p> <a href="https://publications.waset.org/abstracts/19089/introduction-of-pmma-tag-to-vhh-for-improving-recovery-and-immobilization-rate-of-vhhs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19089.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">419</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Experimental and Numerical Studies on Hydrogen Behavior in a Small-Scale Container with Passive Autocatalytic Recombiner</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kazuyuki%20Takase">Kazuyuki Takase</a>, <a href="https://publications.waset.org/abstracts/search?q=Yoshihisa%20Hiraki"> Yoshihisa Hiraki</a>, <a href="https://publications.waset.org/abstracts/search?q=Gaku%20Takase"> Gaku Takase</a>, <a href="https://publications.waset.org/abstracts/search?q=Isamu%20Kudo"> Isamu Kudo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of the most important issue is to ensure the safety of long-term waste storage containers in which fuel debris and radioactive materials are accumulated. In this case, hydrogen generated by water decomposition by radiation is accumulated in the container for a long period of time, so it is necessary to reduce the concentration of hydrogen in the container. In addition, a condition that any power supplies from the outside of the container are unnecessary is requested. Then, radioactive waste storage containers with the passive autocatalytic recombiner (PAR) would be effective. The radioactive waste storage container with PAR was used for moving the fuel debris of the Three Mile Island Unit 2 to the storage location. However, the effect of PAR is not described in detail. Moreover, the reduction of hydrogen concentration during the long-term storage period was performed by the venting system, which was installed on the top of the container. Therefore, development of a long-term storage container with PAR was started with the aim of safely storing fuel debris picked up at the Fukushima Daiichi Nuclear Power Plant for a long period of time. A fundamental experiment for reducing the concentration of hydrogen which generates in a nuclear waste long-term storage container was carried out using a small-scale container with PAR. Moreover, the circulation flow behavior of hydrogen in the small-scale container resulting from the natural convection by the decay heat was clarified. In addition, preliminary numerical analyses were performed to predict the experimental results regarding the circulation flow behavior and the reduction of hydrogen concentration in the small-scale container. From the results of the present study, the validity of the container with PAR was experimentally confirmed on the reduction of hydrogen concentration. In addition, it was predicted numerically that the circulation flow behavior of hydrogen in the small-scale container is blocked by steam which generates by chemical reaction of hydrogen and oxygen. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hydrogen%20behavior" title="hydrogen behavior">hydrogen behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=reduction%20of%20concentration" title=" reduction of concentration"> reduction of concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=long-term%20storage%20container" title=" long-term storage container"> long-term storage container</a>, <a href="https://publications.waset.org/abstracts/search?q=small-scale" title=" small-scale"> small-scale</a>, <a href="https://publications.waset.org/abstracts/search?q=PAR" title=" PAR"> PAR</a>, <a href="https://publications.waset.org/abstracts/search?q=experiment" title=" experiment"> experiment</a>, <a href="https://publications.waset.org/abstracts/search?q=analysis" title=" analysis"> analysis</a> </p> <a href="https://publications.waset.org/abstracts/89008/experimental-and-numerical-studies-on-hydrogen-behavior-in-a-small-scale-container-with-passive-autocatalytic-recombiner" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89008.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">163</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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Search results for: Yoshihisa Hagihara