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<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24"><path class="cls-1" d="M20.79,19.66l-4.58-4.58a7.53,7.53,0,1,0-1.49,1.34l4.66,4.66ZM6.54,14.11a5.56,5.56,0,1,1,3.93,1.62A5.52,5.52,0,0,1,6.54,14.11Z"/> </svg> </span> </div>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_10"); </script> <div class="info-container" id="commerce-bar"> <ul>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_11"); </script> <li id="order-status-1" class="order-status" data-util-index="1" data-analytics-text="Order Status"> <a class="user-utility-link cloud-button" href="/store/orders/solu">Order Status</a> </li>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_12"); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_13"); </script> 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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. Please try again later.", "MINICART_ERROR_2" :"Please enter a quantity in the range of 1 to 999.", "MINICART_ERROR_3" :"Could not add item(s) to cart.", "MINICART_ERROR_4" :"Could not remove item from cart.", "MINICART_ERROR_5" :"Could not create/restore cart.", "MINICART_ERROR_6" :"Unable to retrieve cart details. 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$j('#navList').prepend(activeNav); } </script></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_68b0"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.320.high.jpg/1678381663836.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.480.high.jpg/1678381663836.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.full.high.jpg/1678381663836.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.320.high.jpg/1678381663836.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.480.high.jpg/1678381663836.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.320.medium.jpg/1678381663836.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.620.high.jpg/1678381663836.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.320.low.jpg/1678381663836.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_68b0/foregroundimg.img.480.medium.jpg/1678381663836.jpg" alt='Two lidded-12 well plates with Nunc UpCell surface' title='Nunc UpCell Multiwell Plate'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_68b0 img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_68b0 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_68b0 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p><a href="#nunc-upcell-applications" class="btn btn-featured">Nunc UpCell surface applications</a></p></div><div class="text parbase section"><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/5-step-guide-multilayer-cell-models-upcell-app-note.pdf" target="_blank" class="cta-link"><span class="label label-info">NEW</span> Step-by-step guide on how to create multilayer cell models using Nunc UpCell</a></p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_f947"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.320.low.JPG/1678381663865.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.320.medium.JPG/1678381663865.JPG' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.480.medium.JPG/1678381663865.JPG' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.620.high.JPG/1678381663865.JPG' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.full.high.JPG/1678381663865.JPG' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.full.high.JPG/1678381663865.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.320.high.JPG/1678381663865.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.480.high.JPG/1678381663865.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.320.medium.JPG/1678381663865.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.620.high.JPG/1678381663865.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.320.low.JPG/1678381663865.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_f947/foregroundimg.img.480.medium.JPG/1678381663865.JPG" alt='Three plates at different temperatures. At 37°C, cells adhere to the dish, at 20°C, cells are available for harvesting' title='UpCell surface for non-enzymatic cell dissociation'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_f947 img{ width:100%; max-width: 1676px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_f947 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_f947 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><h2>Why use UpCell dishes for adherent cell culture?</h2><p><b>With UpCell surfaces:</b></p><ul><li>There’s no need for trypsin—UpCell non-enzymatic surfaces mean better preservation of cell surface proteins</li><li>There’s no need for scraping—UpCell surfaces promote improved cell viability</li><li>Plastics are designed for culture passaging, single-cell analyses, and cell transplantation research</li><li>With supportive membrane options, UpCell surfaces enable harvesting of cell sheets and the creation of 3D tissue models</li></ul></div></div></div></div><div style="clear:both"></div><div class="accordion section"><div class="accordion" id="bce09a14760d4e43a25fc088ca3fe532" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#bce09a14760d4e43a25fc088ca3fe532" href="#nuncupcell1"><span class="caret"></span> Nunc UpCell surface-mediated dissociation compared to trypsin </a></div><div class="accordion-body collapse " id="nuncupcell1"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><h3>UpCell surfaces and trypsin: cell viability</h3><p>Despite longer dissociation time, UpCell surfaces exhibit minimal impact on cell viability. These right-hand graphs show mesenchymal stem cells (MSCs) differential viability retention when MSCs, treated with either trypsin on the Nunclon Delta Surface or dissociated by temperature shift on the Nunc UpCell surface, were left until completely detached.</p><p><b>(A) </b>Dissociation time was noted.<br><b>(B) </b>Cell viability was measured using the <a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Countess II Automated Cell Counter.</a></p><div class="alert alert-info"><p><b>Please note:</b> Individual experiments were done in duplicate, and data are represented as the mean ± SEM. ns: not significant, *: P < 0.05 (two-tailed unpaired t-test)</p></div><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/cell-harvesting-nunc-upcell-surface-brochure.pdf" class="cta-link" target="_blank"><span class="icon-lt-pdf"></span> Explore more comparative results between trypsinization and UpCell</a></p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2a17"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.320.medium.JPG/1678381663959.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.480.high.JPG/1678381663959.JPG' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.620.high.JPG/1678381663959.JPG' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.full.high.JPG/1678381663959.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.320.high.JPG/1678381663959.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.480.high.JPG/1678381663959.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.320.medium.JPG/1678381663959.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.620.high.JPG/1678381663959.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.320.low.JPG/1678381663959.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2a17/foregroundimg.img.480.medium.JPG/1678381663959.JPG" alt='A bar chart in reds describing Nunc UpCell surface’s longer cell dissociation time' title='Comparative time taken to dissociate cells'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2a17 img{ width:100%; max-width: 683px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2a17 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2a17 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt7" comp-id="B2"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2926"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2926/foregroundimg.img.320.low.JPG/1678381663979.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2926/foregroundimg.img.320.medium.JPG/1678381663979.JPG' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2926/foregroundimg.img.480.medium.JPG/1678381663979.JPG' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_b469/itemParsys/image_2926/foregroundimg.img.620.high.JPG/1678381663979.JPG' media="(min-width: 630px)"><source 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alt='A bar chart in reds describing Nunc UpCell surface’s superior ability for cell viability retention even as harvesting takes longer by UpCell surface.' title='Comparative cell viability'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2926 img{ width:100%; max-width: 1115px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2926 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_2926 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><h3>UpCell surfaces and trypsin: protein expression</h3><p>With measurements of CD44, CD105, and CD13 fluorescence intensity in MSCs after antibody staining, results indicate that UpCell surfaces have milder effects on cultured MSCs, preserving cell-surface antigens and maintaining high viability when compared to trypsinization.</p><p><b>(C)</b> Representative flow cytometry histograms showing expression of CD44 following dissociation using 0.25% trypsin or the Nunc UpCell Surface.<br><b>(D)</b> Median fluorescence intensities of surface antigens are demonstrated as bar graphs.</p><div class="alert alert-info"><p><b>Please note:</b> Individual experiments were done in duplicate, and data are represented as the mean ± SEM. ns: not significant, **: P < 0.005, *: P < 0.05 (two-tailed unpaired t-test).</p></div><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/cell-harvesting-nunc-upcell-surface-brochure.pdf" class="cta-link" target="_blank"><span class="icon-lt-pdf"></span> Explore comparative results for CD105 and CD13</a></p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_f64e"><picture><source 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alt='Histogram of count vs. log fluorescence intensity for CD44 staining following trypsinization vs UpCell surface' title='Flow cytometry'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_f64e img{ width:100%; max-width: 565px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_f64e .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_f64e .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text 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alt='A log fluorescence intensity assay describing superior retention of CD44 antigens in MSCs after dissociation by UpCell Surface.' title='Fluorescence assay for CD44 on UpCell surface'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_193 img{ width:100%; max-width: 696px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_193 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_b469itemParsysimage_193 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#bce09a14760d4e43a25fc088ca3fe532" href="#nuncupcell2"><span class="caret"></span> Nunc UpCell surface-mediated dissociation compared to cell scraping </a></div><div class="accordion-body collapse " id="nuncupcell2"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><h3>UpCell surfaces and scraping: cell recovery</h3><p>Recovery ratios of mouse peritoneal macrophages harvested from the Nunc UpCell surfaces were compared with recovery ratios of these cells harvested by either enzymatic (trypsinization) or mechanical (scraping) methods.</p><p>The recovery of cells from the Nunc UpCell surface was significantly higher than the recovery of cells harvested from traditional cultureware by trypsinization or scraping (see figure).</p><div class="alert alert-info"><p><b>Please note:</b> Mean and SD are shown.</p></div><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/cell-harvesting-nunc-upcell-surface-brochure.pdf" class="cta-link" target="_blank"><span class="icon-lt-pdf"></span> Explore more comparative results between cell scraping and UpCell</a></p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_1066itemParsysimage_f909"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_1066/itemParsys/image_f909/foregroundimg.img.320.low.JPG/1678381664116.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_644b/itemspar/accordionitem_1066/itemParsys/image_f909/foregroundimg.img.320.medium.JPG/1678381664116.JPG' media="(min-width: 320px)"><source 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alt='A bar chart of recovery ratio for UpCell surface compared to trypsinization or cell scraping' title='Differential cell recovery via cell harvesting methods'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_1066itemParsysimage_f909 img{ width:100%; max-width: 1239px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_1066itemParsysimage_f909 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysaccordion_644bitemsparaccordionitem_1066itemParsysimage_f909 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#bce09a14760d4e43a25fc088ca3fe532').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#bce09a14760d4e43a25fc088ca3fe532').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><p><a href="#surface-plates-dishes" class="cta-link">Explore Nunc UpCell cultureware</a></p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><h2>How the Nunc UpCell surface works</h2><p>The Nunc UpCell surface is designed to be temperature responsive, enabling non-enzymatic cell dissociation. This surface is composed of a covalently immobilized polymer, PIPAAm. PIPAAm forms a thin, even coating over the cultureware and, being slightly hydrophobic at 37°C, allows cells to attach and grow. However, when the cultureware’s temperature reduces below 32°C, this same layer becomes hydrophilic. In this state, PIPAAm binds water, swells, and results in adherent cell release.</p><p><a href="#order" class="btn btn-primary">Order Nunc UpCell surface cultureware</a></p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_71a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_71a/foregroundimg.img.320.low.JPG/1678381664157.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/image_71a/foregroundimg.img.320.medium.JPG/1678381664157.JPG' media="(min-width: 320px)"><source 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alt='At 37°C, UpCell surfaces are hydrophobic, allowing cell adhesion, below 32°C, the UpCell surface becomes hydrophilic, binds water, swells, and releases cells.' title='How Nunc UpCell Surfaces work'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_71a img{ width:100%; max-width: 1584px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_71a .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsysimage_71a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2 id="nunc-upcell-applications"><br>Nunc UpCell surface applications </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-cont1">Cell-surface<br>protein preservation</a></li><li><a data-toggle="tab" href="#container-cont2">Single-cell<br>harvesting</a></li><li><a data-toggle="tab" href="#container-cont3">Cell sheet<br>harvesting</a></li><li><a data-toggle="tab" href="#container-cont4">Cell sheet<br>engineering</a></li><li><a data-toggle="tab" href="#container-cont5">Quality<br>assurance</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-cont1" class="tab-pane active"><div class="container-cont1 tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><h3>Maximize cell-surface protein preservation</h3><p>While many surface proteins are critical for the cell’s response to extracellular matrices, to other cells, and to growth factors, as well as other soluble mediators, enzymatic cell harvesting such as trypsinization compromises these proteins’ integrity.</p><p>The right-shifting of gray peaks (see figure) indicate that CD140a, a cell-surface molecule, is preserved through temperature-reduction harvesting on the Nunc UpCell surface as compared with trypsinization.</p><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/advantages-upcell-surface-over-trypsin-app-note.pdf" class="cta-link" target="_blank"><span class="icon-lt-pdf"></span> Read more about UpCell surface's cell-surface-protein preservation</a></p></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.low.JPG/1678381664528.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.medium.JPG/1678381664528.JPG' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.480.medium.JPG/1678381664528.JPG' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.620.high.JPG/1678381664528.JPG' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.full.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.480.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.medium.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.620.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.low.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.480.medium.JPG/1678381664528.JPG" alt=': four histograms describing how CD140a, a cell surface molecule, is preserved through UpCell-surface-mediated harvesting as compared with trypsinization.' title='UpCell surfaces for protein retention'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2 img{ width:100%; max-width: 836px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div><div id="container-cont2" class="tab-pane"><div class="container-cont2 tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><h4>How to use UpCell surfaces for single-cell harvesting</h4><p>Nunc UpCell enables high-viability harvesting, keeping cell surface receptors and antigens intact. This includes finicky cells, like primary cells, which prove difficult to passage by other methods.</p><p><b>Single-cell suspensions harvested from cultureware with UpCell surfaces can be utilized by: </b></p><ul><li>Analysis <i>in vitro</i> (i.e., by flow cytometry)</li><li>Manipulation to purify certain cell types</li><li>Re-seeding in cultureware with UpCell surfaces or traditional cultureware as a part of passaging procedures</li><li>Researching cell transplantation</li></ul><p><b>To harvest single cells from UpCell surface-treated cultureware:</b></p><ol><li>Seed cells in your UpCell surface-treated cultureware</li><li>Plate at 37°C</li><li>Grow cells up to 70% confluence (depending on cell type)</li><li>Place cultureware in 20–25°C environment for 10–40 minutes (depending on cell type)</li><li>And harvest your cells!</li></ol></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.full.high.png/1678381664459.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.full.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.320.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.480.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.320.medium.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.620.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.320.low.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.480.medium.png/1678381664459.png" alt='Step by step instructional on how to harvest single cells with UpCell cultureware' title='UpCell surfaces for single cell harvesting'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d img{ width:100%; max-width: 211px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h3>Citations for single-cell suspension harvesting with UpCell surfaces</h3><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" class="rte-table" id="table--1689924513"><tbody><tr><th scope="col">Cell type</th><th scope="col">Application</th><th scope="col">Reference</th></tr><tr><td>Microglia (rat)</td><td width="208" valign="top"><p>Analysis (detachment and function)</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/11438391/" target="_blank">Nakajima et al., 2001</a></td></tr><tr><td>Monocytes and macrophages (human)</td><td>Re-seeding/passaging</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/11920674/" target="_blank">Collier et al., 2002</a></td></tr><tr><td>Monocytes and macrophages (human)</td><td>Activation and analysis (structural)</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16533776/" target="_blank">Gordon and Freedman, 2006</a></td></tr><tr><td>Basophilic cell line RBL-2H3 (rat)</td><td>Antigen-mediated degranulation measured by surface plasmon resonance</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16621506/" target="_blank">Yanase et al., 2007</a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-cont3" class="tab-pane"><div class="container-cont3 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3>How to use UpCell surfaces for cell-sheet harvesting</h3><p>Anchorage-dependent cells are ordinarily held together by a deposited subcellular matrix as well as by cell junctions attached to medium-derived and cultureware-absorbed proteins. When using traditional enzymatic and/or mechanical harvesting methods, these cell-to-cell, and cell-to-matrix contacts, as well as the subcellular matrix itself, are disrupted—their polarization destroyed.</p></div><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><p>However, with Nunc UpCell surface and supplied support membranes, cells can be detached as contiguous sheets, avoiding this disruption. Membranes are supplied with <a href="/order/catalog/product/174901">Nunc Multidishes with UpCell Surface (Cat. No. 174901)</a> and <a href="/order/catalog/product/174904">Nunc Dishes with UpCell Surface (Cat. No. 174904)</a> only. </p><p><b>Cell sheets harvested from UpCell surfaces can be utilized by:</b></p><ul><li>Analysis <i>in vitro</i> (i.e., electron microscopy)</li><li>Re-plating into cultureware with UpCell surfaces or into traditional cultureware</li><li>Transplantation models</li><li>Cell sheet stacking/engineering</li></ul><p><b>To harvest cell sheets from UpCell surfaces:</b></p><ol><li>Grow cells to confluence on UpCell surface</li><li>Aspirate/add fresh medium</li><li>Place provided membrane on top of cell layer</li><li>Place cultureware in 20–25°C environment for 5–6 minutes</li><li>Manually detach cell layer around membrane edge</li><li>Withdraw membrane (with cell layer)</li><li>Transfer membrane (with cell layer) to new cultureware/graft site</li><li>Remove membrane</li></ol></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.full.high.png/1678381664385.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.full.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.320.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.480.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.320.medium.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.620.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.320.low.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.480.medium.png/1678381664385.png" alt='Step by step instructional on how to harvest cell sheets with UpCell cultureware' title='UpCell surfaces for cell-sheet harvesting'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9 img{ width:100%; max-width: 247px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h3>Citations for cell sheet harvesting with UpCell surfaces</h3><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" class="rte-table" id="table-1334176229"><tbody><tr><th scope="col">Cell type</th><th scope="col">Application</th><th scope="col">Reference</th></tr><tr><td>Urothelial cells (human)</td><td width="208" valign="top"><p>Analysis (electron microscopy)</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/14633384/" target="_blank">Shiroyanagi et al., 2003 <span class="icon icon-outbound"></span></a><br></td></tr><tr><td>Retinal pigment epithelial cell line ARPE-19 (human)</td><td>Analysis (light microscopy)</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16545450/" target="_blank">Kubota et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Kidney epithelial cells (human and dog)</td><td>Re-plating to traditional cultureware and analysis (electron and fluorescence microscopy)</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16088852/" target="_blank">Kushida et al., 2005 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Mesenchymal stem cells and skin fibroblasts (rat)</td><td>Analysis (structural and functional) and transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16582917/" target="_blank">Miyahara et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Corneal endothelial cells (human)</td><td>Analysis (structural and functional) and transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16339916/" target="_blank">Sumide et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Oral mucosal epithelial cells (dog)</td><td width="208" valign="top"><p>Analysis (structural) and transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16709659/" target="_blank">Ohki et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Tracheal epithelial cells (rabbit)</td><td width="208" valign="top"><p>Transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16771640/" target="_blank">Kanzaki et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Periodontal ligament cells (human)</td><td width="208" valign="top"><p>Transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/15869425/" target="_blank">Hasegawa et al., 2005 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Mesenchymal stem cells (human)</td><td width="208" valign="top"><p>Transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/32069701/" target="_blank">Cho et al., 2020 <span class="icon icon-outbound"></span></a><br></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-cont4" class="tab-pane"><div class="container-cont4 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3><a>How to use UpCell surfaces for 3D tissue models</a></h3><p>Ordinarily, 3D tissue models or transplants are prepared by seeding cell suspensions on prefabricated scaffolds. These scaffold’s materials are not ordinarily cell produced. Instead, foreign, or xenogeneic materials, such as polylactic acid (PLA), polyglycolic acid (PGA), alginate, gelatin, and collagen are often utilized.</p></div><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><p>With this procedure, problems such as uneven cell distribution, special distribution control difficulties, host inflammatory reactions, or fibrous tissue formation can arise.</p><p>To mitigate these risks, UpCell surfaces enable harvested cell sheets to be stacked to form 3D tissue models—sans xenogeneic scaffold.</p><p><b>These Nunc UpCell surface cell sheet constructs can be:</b></p><ul><li>Analyzed <i>in vitro</i> (i.e., by functional tissue-specific tests)</li><li>Cultivated <i>in vitro</i> (i.e., as 3D co-cultures)</li><li>Used in different transplantation models, where cell sheets are stacked before or during the transplantation procedure</li></ul><p><b>To engineer cell sheets with UpCell surfaces:</b></p><ol><li>Transfer UpCell surface–harvested membrane (with cell sheet) to a confluent culture with aspirated medium</li><li>Add fresh medium</li><li>Place cultureware in a 37°C environment for ~30 minutes</li><li>Withdraw membrane (without cell layer)</li><li>Add fresh medium and incubate at 37°C</li></ol><p><b>OR</b></p><p>Aspirate medium and transfer another cell sheet to construct.</p></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.full.high.png/1678381664308.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.full.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.320.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.480.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.320.medium.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.620.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.320.low.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.480.medium.png/1678381664308.png" alt='Step by step instructional on how to engineer cell sheets with UpCell cultureware' title='UpCell surfaces for cell-sheet engineering'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b img{ width:100%; max-width: 239px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h3><a>Citations for cell sheet engineering with UpCell surfaces</a></h3><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" class="rte-table" id="table-1736499051"><tbody><tr><th scope="col">Cell type</th><th scope="col">Application</th><th scope="col">Reference</th></tr><tr><td>Aortic endothelial cells (human)</td><td width="208" valign="top"><p>Cultivation (3D co-culture) & analysis (structural)</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/12209933/" target="_blank">Harimoto et al., 2002 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Hepatocytes (mouse and human)</td><td>Analysis (structural and functional) & stacking during transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/17572687/" target="_blank">Ohashi et al., 2007 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Skeletal myoblast (dog)</td><td>Analysis (structural) & stacking during transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/17000305/" target="_blank">Hata et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Lung and skin fibroblasts (rat)</td><td>Analysis (structural) & stacking during transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/17602737/" target="_blank">Kanzaki et al., 2007 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Cardiomyocytes (rat)</td><td>Cultivation & analysis (structural and functional) & stacking before transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16434025/" target="_blank">Sekiya et al., 2006 <span class="icon icon-outbound"></span></a>, <a></a><a href="https://pubmed.ncbi.nlm.nih.gov/11861428/" target="_blank">Shimizu et al., 2002 <span class="icon icon-outbound"></span></a>, and <a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16579683/" target="_blank">Shimizu et al., 2006 <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-cont5" class="tab-pane"><div class="container-cont5 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3>Quality is inherent in our culture</h3><p>From product development and sourcing raw materials to manufacturing and customer service, quality is reflected in every Nunc product.</p><p>A certificate of quality is packed in each box of cultureware with Nunc UpCell surface. This certificate is your confirmation that the product has been validated according to the following tests:</p><h3>Cell growth</h3><p>Each manufacturing lot is sampled and subjected to performance testing for growth with the 3T3-Swiss Albino cell line (derived from a mouse embryo fibroblast) in accordance with standard operating procedures.</p><p>Acceptance level: minimum of 80% confluence.</p><h3>Cell detachment</h3><p>The manufacturing lot is sampled and subjected to performance testing for cell detachment by temperature reduction with the 3T3-Swiss Albino cell line in accordance with standard operating procedures. Adherent cells are detached by temperature decreasing treatment (below 32°C) and the degree of detachment is measured.</p><p>Acceptance level: detachment of 50% or more of the cells.</p><h3>Sterility</h3><p>Sterility is obtained by using ethylene oxide gas according to ISO 11135-1 guidelines:<br>(Sterilization of health care products, Ethylene oxide, Part 1: Requirements for development, validation, and routine control of a sterilization process for medical devices).</p></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> Cell-surface<br>protein preservation </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-cont1"><div class="container-cont1 tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><h3>Maximize cell-surface protein preservation</h3><p>While many surface proteins are critical for the cell’s response to extracellular matrices, to other cells, and to growth factors, as well as other soluble mediators, enzymatic cell harvesting such as trypsinization compromises these proteins’ integrity.</p><p>The right-shifting of gray peaks (see figure) indicate that CD140a, a cell-surface molecule, is preserved through temperature-reduction harvesting on the Nunc UpCell surface as compared with trypsinization.</p><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/advantages-upcell-surface-over-trypsin-app-note.pdf" class="cta-link" target="_blank"><span class="icon-lt-pdf"></span> Read more about UpCell surface's cell-surface-protein preservation</a></p></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.low.JPG/1678381664528.JPG' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.medium.JPG/1678381664528.JPG' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.480.medium.JPG/1678381664528.JPG' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.620.high.JPG/1678381664528.JPG' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.full.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.480.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.medium.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.620.high.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.320.low.JPG/1678381664528.JPG,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont1/tab/image_7fb2/foregroundimg.img.480.medium.JPG/1678381664528.JPG" alt=': four histograms describing how CD140a, a cell surface molecule, is preserved through UpCell-surface-mediated harvesting as compared with trypsinization.' title='UpCell surfaces for protein retention'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2 img{ width:100%; max-width: 836px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont1tabimage_7fb2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> Single-cell<br>harvesting </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-cont2"><div class="container-cont2 tabstep"><div class="start"></div><div class="tab parsys"><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><h4>How to use UpCell surfaces for single-cell harvesting</h4><p>Nunc UpCell enables high-viability harvesting, keeping cell surface receptors and antigens intact. This includes finicky cells, like primary cells, which prove difficult to passage by other methods.</p><p><b>Single-cell suspensions harvested from cultureware with UpCell surfaces can be utilized by: </b></p><ul><li>Analysis <i>in vitro</i> (i.e., by flow cytometry)</li><li>Manipulation to purify certain cell types</li><li>Re-seeding in cultureware with UpCell surfaces or traditional cultureware as a part of passaging procedures</li><li>Researching cell transplantation</li></ul><p><b>To harvest single cells from UpCell surface-treated cultureware:</b></p><ol><li>Seed cells in your UpCell surface-treated cultureware</li><li>Plate at 37°C</li><li>Grow cells up to 70% confluence (depending on cell type)</li><li>Place cultureware in 20–25°C environment for 10–40 minutes (depending on cell type)</li><li>And harvest your cells!</li></ol></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.full.high.png/1678381664459.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.full.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.320.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.480.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.320.medium.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.620.high.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.320.low.png/1678381664459.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont2/tab/image_4f6d/foregroundimg.img.480.medium.png/1678381664459.png" alt='Step by step instructional on how to harvest single cells with UpCell cultureware' title='UpCell surfaces for single cell harvesting'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d img{ width:100%; max-width: 211px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont2tabimage_4f6d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h3>Citations for single-cell suspension harvesting with UpCell surfaces</h3><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" class="rte-table" id="table--674166634"><tbody><tr><th scope="col">Cell type</th><th scope="col">Application</th><th scope="col">Reference</th></tr><tr><td>Microglia (rat)</td><td width="208" valign="top"><p>Analysis (detachment and function)</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/11438391/" target="_blank">Nakajima et al., 2001</a></td></tr><tr><td>Monocytes and macrophages (human)</td><td>Re-seeding/passaging</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/11920674/" target="_blank">Collier et al., 2002</a></td></tr><tr><td>Monocytes and macrophages (human)</td><td>Activation and analysis (structural)</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16533776/" target="_blank">Gordon and Freedman, 2006</a></td></tr><tr><td>Basophilic cell line RBL-2H3 (rat)</td><td>Antigen-mediated degranulation measured by surface plasmon resonance</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16621506/" target="_blank">Yanase et al., 2007</a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> Cell sheet<br>harvesting </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-cont3"><div class="container-cont3 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3>How to use UpCell surfaces for cell-sheet harvesting</h3><p>Anchorage-dependent cells are ordinarily held together by a deposited subcellular matrix as well as by cell junctions attached to medium-derived and cultureware-absorbed proteins. When using traditional enzymatic and/or mechanical harvesting methods, these cell-to-cell, and cell-to-matrix contacts, as well as the subcellular matrix itself, are disrupted—their polarization destroyed.</p></div><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><p>However, with Nunc UpCell surface and supplied support membranes, cells can be detached as contiguous sheets, avoiding this disruption. Membranes are supplied with <a href="/order/catalog/product/174901">Nunc Multidishes with UpCell Surface (Cat. No. 174901)</a> and <a href="/order/catalog/product/174904">Nunc Dishes with UpCell Surface (Cat. No. 174904)</a> only. </p><p><b>Cell sheets harvested from UpCell surfaces can be utilized by:</b></p><ul><li>Analysis <i>in vitro</i> (i.e., electron microscopy)</li><li>Re-plating into cultureware with UpCell surfaces or into traditional cultureware</li><li>Transplantation models</li><li>Cell sheet stacking/engineering</li></ul><p><b>To harvest cell sheets from UpCell surfaces:</b></p><ol><li>Grow cells to confluence on UpCell surface</li><li>Aspirate/add fresh medium</li><li>Place provided membrane on top of cell layer</li><li>Place cultureware in 20–25°C environment for 5–6 minutes</li><li>Manually detach cell layer around membrane edge</li><li>Withdraw membrane (with cell layer)</li><li>Transfer membrane (with cell layer) to new cultureware/graft site</li><li>Remove membrane</li></ol></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.full.high.png/1678381664385.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.full.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.320.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.480.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.320.medium.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.620.high.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.320.low.png/1678381664385.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont3/tab/image_7da9/foregroundimg.img.480.medium.png/1678381664385.png" alt='Step by step instructional on how to harvest cell sheets with UpCell cultureware' title='UpCell surfaces for cell-sheet harvesting'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9 img{ width:100%; max-width: 247px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9 .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont3tabimage_7da9 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h3>Citations for cell sheet harvesting with UpCell surfaces</h3><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" class="rte-table" id="table--1622545727"><tbody><tr><th scope="col">Cell type</th><th scope="col">Application</th><th scope="col">Reference</th></tr><tr><td>Urothelial cells (human)</td><td width="208" valign="top"><p>Analysis (electron microscopy)</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/14633384/" target="_blank">Shiroyanagi et al., 2003 <span class="icon icon-outbound"></span></a><br></td></tr><tr><td>Retinal pigment epithelial cell line ARPE-19 (human)</td><td>Analysis (light microscopy)</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16545450/" target="_blank">Kubota et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Kidney epithelial cells (human and dog)</td><td>Re-plating to traditional cultureware and analysis (electron and fluorescence microscopy)</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16088852/" target="_blank">Kushida et al., 2005 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Mesenchymal stem cells and skin fibroblasts (rat)</td><td>Analysis (structural and functional) and transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16582917/" target="_blank">Miyahara et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Corneal endothelial cells (human)</td><td>Analysis (structural and functional) and transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16339916/" target="_blank">Sumide et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Oral mucosal epithelial cells (dog)</td><td width="208" valign="top"><p>Analysis (structural) and transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16709659/" target="_blank">Ohki et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Tracheal epithelial cells (rabbit)</td><td width="208" valign="top"><p>Transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16771640/" target="_blank">Kanzaki et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Periodontal ligament cells (human)</td><td width="208" valign="top"><p>Transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/15869425/" target="_blank">Hasegawa et al., 2005 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Mesenchymal stem cells (human)</td><td width="208" valign="top"><p>Transplantation</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/32069701/" target="_blank">Cho et al., 2020 <span class="icon icon-outbound"></span></a><br></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainer" href="#collapse4"><span class="caret"></span><strong> Cell sheet<br>engineering </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-cont4"><div class="container-cont4 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3><a>How to use UpCell surfaces for 3D tissue models</a></h3><p>Ordinarily, 3D tissue models or transplants are prepared by seeding cell suspensions on prefabricated scaffolds. These scaffold’s materials are not ordinarily cell produced. Instead, foreign, or xenogeneic materials, such as polylactic acid (PLA), polyglycolic acid (PGA), alginate, gelatin, and collagen are often utilized.</p></div><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="text parbase section"><p>With this procedure, problems such as uneven cell distribution, special distribution control difficulties, host inflammatory reactions, or fibrous tissue formation can arise.</p><p>To mitigate these risks, UpCell surfaces enable harvested cell sheets to be stacked to form 3D tissue models—sans xenogeneic scaffold.</p><p><b>These Nunc UpCell surface cell sheet constructs can be:</b></p><ul><li>Analyzed <i>in vitro</i> (i.e., by functional tissue-specific tests)</li><li>Cultivated <i>in vitro</i> (i.e., as 3D co-cultures)</li><li>Used in different transplantation models, where cell sheets are stacked before or during the transplantation procedure</li></ul><p><b>To engineer cell sheets with UpCell surfaces:</b></p><ol><li>Transfer UpCell surface–harvested membrane (with cell sheet) to a confluent culture with aspirated medium</li><li>Add fresh medium</li><li>Place cultureware in a 37°C environment for ~30 minutes</li><li>Withdraw membrane (without cell layer)</li><li>Add fresh medium and incubate at 37°C</li></ol><p><b>OR</b></p><p>Aspirate medium and transfer another cell sheet to construct.</p></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="image parbase section"><div id="cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.full.high.png/1678381664308.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.full.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.320.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.480.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.320.medium.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.620.high.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.320.low.png/1678381664308.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/tabworkflowcontainer/container-cont4/tab/image_cb0b/foregroundimg.img.480.medium.png/1678381664308.png" alt='Step by step instructional on how to engineer cell sheets with UpCell cultureware' title='UpCell surfaces for cell-sheet engineering'></picture><style> #cq-image-jsp-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b img{ width:100%; max-width: 239px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b .image-contain" + "," + "#cq-image-jsp-"+"c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainercontainer-cont4tabimage_cb0b .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h3><a>Citations for cell sheet engineering with UpCell surfaces</a></h3><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" class="rte-table" id="table--763584053"><tbody><tr><th scope="col">Cell type</th><th scope="col">Application</th><th scope="col">Reference</th></tr><tr><td>Aortic endothelial cells (human)</td><td width="208" valign="top"><p>Cultivation (3D co-culture) & analysis (structural)</p></td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/12209933/" target="_blank">Harimoto et al., 2002 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Hepatocytes (mouse and human)</td><td>Analysis (structural and functional) & stacking during transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/17572687/" target="_blank">Ohashi et al., 2007 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Skeletal myoblast (dog)</td><td>Analysis (structural) & stacking during transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/17000305/" target="_blank">Hata et al., 2006 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Lung and skin fibroblasts (rat)</td><td>Analysis (structural) & stacking during transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/17602737/" target="_blank">Kanzaki et al., 2007 <span class="icon icon-outbound"></span></a></td></tr><tr><td>Cardiomyocytes (rat)</td><td>Cultivation & analysis (structural and functional) & stacking before transplantation</td><td><a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16434025/" target="_blank">Sekiya et al., 2006 <span class="icon icon-outbound"></span></a>, <a></a><a href="https://pubmed.ncbi.nlm.nih.gov/11861428/" target="_blank">Shimizu et al., 2002 <span class="icon icon-outbound"></span></a>, and <a></a><a href="https://pubmed.ncbi.nlm.nih.gov/16579683/" target="_blank">Shimizu et al., 2006 <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-c29a5851-33dd-44c3-8c6f-6ae2bc7f74e6MainParsystabworkflowcontainer" href="#collapse5"><span class="caret"></span><strong> Quality<br>assurance </strong></a></div><div id="collapse5" class="accordion-body collapse "><div class="accordion-inner container-cont5"><div class="container-cont5 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3>Quality is inherent in our culture</h3><p>From product development and sourcing raw materials to manufacturing and customer service, quality is reflected in every Nunc product.</p><p>A certificate of quality is packed in each box of cultureware with Nunc UpCell surface. This certificate is your confirmation that the product has been validated according to the following tests:</p><h3>Cell growth</h3><p>Each manufacturing lot is sampled and subjected to performance testing for growth with the 3T3-Swiss Albino cell line (derived from a mouse embryo fibroblast) in accordance with standard operating procedures.</p><p>Acceptance level: minimum of 80% confluence.</p><h3>Cell detachment</h3><p>The manufacturing lot is sampled and subjected to performance testing for cell detachment by temperature reduction with the 3T3-Swiss Albino cell line in accordance with standard operating procedures. Adherent cells are detached by temperature decreasing treatment (below 32°C) and the degree of detachment is measured.</p><p>Acceptance level: detachment of 50% or more of the cells.</p><h3>Sterility</h3><p>Sterility is obtained by using ethylene oxide gas according to ISO 11135-1 guidelines:<br>(Sterilization of health care products, Ethylene oxide, Part 1: Requirements for development, validation, and routine control of a sterilization process for medical devices).</p></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="order"><br>Order Nunc UpCell surface plates and dishes </h2></div><div class="accordion section"><div class="accordion" id="415f50df00454612b979b60af2a2f4aa" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#415f50df00454612b979b60af2a2f4aa" href="#dishes1"><span class="caret"></span> Order Nunc UpCell dishes </a></div><div class="accordion-body collapse " id="dishes1"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">174902,174903,174904,174905,174906</div><div id='ltajax-7055980455702249727'></div><script type="text/javascript">(function() {$('#ltajax-7055980455702249727').load('/sg/en/home/life-science/cell-culture/cell-culture-plastics/nunc-upcell-surfaces-adherent-cell-culture/jcr:content/MainParsys/accordion_cc79/itemspar/accordionitem_1db2/itemParsys/productlist_438e.ajax.html?skulist=');})(); 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