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overflow: hidden; text-overflow: ellipsis; -webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">immunoglobulin G</h1><div class="u-tcGrayDark">75 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>immunoglobulin G</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/immunoglobulin_G">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/immunoglobulin_G/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/immunoglobulin_G/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/immunoglobulin_G/MostRecent">Newest Papers</a></li><li><a class="" href="https://www.academia.edu/People/immunoglobulin_G">People</a></li></ul></div><style type="text/css">ul.nav{flex-direction:row}@media(max-width: 567px){ul.nav{flex-direction:column}.TabbedNavigation li{max-width:100%}.TabbedNavigation li.active{background-color:var(--background-grey, #dddde2)}.TabbedNavigation li.active:before,.TabbedNavigation li.active:after{display:none}}</style></div></div></div><div class="container"><div class="row"><div class="col-xs-12"><div class="u-displayFlex"><div class="u-flexGrow1"><div class="works"><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24101187" data-work_id="24101187" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24101187/In_Vivo_Inhibition_of_Anti_Hepatitis_B_Virus_Core_Antigen_HBcAg_Immunoglobulin_G_Production_by_HBcAg_Specific_CD4_Th1_Type_T_Cell_Clones_in_a_hu_PBL_NOD_SCID_Mouse_Model">In Vivo Inhibition of Anti-Hepatitis B Virus Core Antigen (HBcAg) Immunoglobulin G Production by HBcAg-Specific CD4+ Th1-Type T-Cell Clones in a hu-PBL-NOD/SCID Mouse Model</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">؉ T-cell responses are believed to play an important role in the control of human HBV infection. In the present study, HBcAg-specific, HLA-DR13*-restricted CD4 ؉ Th1-type T-cell clones were generated which secreted both gamma interferon... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24101187" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">؉ T-cell responses are believed to play an important role in the control of human HBV infection. In the present study, HBcAg-specific, HLA-DR13*-restricted CD4 ؉ Th1-type T-cell clones were generated which secreted both gamma interferon and tumor necrosis factor alpha after in vitro antigen stimulation. These HBcAg-specific CD4 ؉ Th1-type T cells were able to lyse HBc peptide-loaded Epstein-Barr virus-transformed lymphoblastoid target cells in vitro. To examine whether these HLA-DR13*-restricted human CD4 ؉ Th1 T cells also display the same cytotoxic effects in vivo, we transferred peripheral blood leukocytes (PBL) derived from HBV-infected donors or an HBV-naïve donor sharing the DR13*, together with the HBcAg-specific CD4 ؉ Th1-type T</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24101187" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0fa3cde418f5510c53c70b10ed6d31bf" rel="nofollow" data-download="{"attachment_id":44465449,"asset_id":24101187,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44465449/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33770610" href="https://independent.academia.edu/IsabelleDesombere">Isabelle Desombere</a><script data-card-contents-for-user="33770610" type="text/json">{"id":33770610,"first_name":"Isabelle","last_name":"Desombere","domain_name":"independent","page_name":"IsabelleDesombere","display_name":"Isabelle Desombere","profile_url":"https://independent.academia.edu/IsabelleDesombere?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24101187 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24101187"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24101187, container: ".js-paper-rank-work_24101187", }); 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In the present study, HBcAg-specific, HLA-DR13*-restricted CD4 ؉ Th1-type T-cell clones were generated which secreted both gamma interferon and tumor necrosis factor alpha after in vitro antigen stimulation. These HBcAg-specific CD4 ؉ Th1-type T cells were able to lyse HBc peptide-loaded Epstein-Barr virus-transformed lymphoblastoid target cells in vitro. To examine whether these HLA-DR13*-restricted human CD4 ؉ Th1 T cells also display the same cytotoxic effects in vivo, we transferred peripheral blood leukocytes (PBL) derived from HBV-infected donors or an HBV-naïve donor sharing the DR13*, together with the HBcAg-specific CD4 ؉ Th1-type T","downloadable_attachments":[{"id":44465449,"asset_id":24101187,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33770610,"first_name":"Isabelle","last_name":"Desombere","domain_name":"independent","page_name":"IsabelleDesombere","display_name":"Isabelle Desombere","profile_url":"https://independent.academia.edu/IsabelleDesombere?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=1716403","nofollow":false},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology?f_ri=1716403","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false},{"id":63331,"name":"Hepatitis B","url":"https://www.academia.edu/Documents/in/Hepatitis_B?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403"},{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=1716403"},{"id":315624,"name":"Epstein-Barr virus","url":"https://www.academia.edu/Documents/in/Epstein-Barr_virus?f_ri=1716403"},{"id":323415,"name":"Mouse Model","url":"https://www.academia.edu/Documents/in/Mouse_Model?f_ri=1716403"},{"id":816379,"name":"Virus infection","url":"https://www.academia.edu/Documents/in/Virus_infection?f_ri=1716403"},{"id":825565,"name":"Hepatitis B virus","url":"https://www.academia.edu/Documents/in/Hepatitis_B_virus?f_ri=1716403"},{"id":855510,"name":"Liver Disease","url":"https://www.academia.edu/Documents/in/Liver_Disease?f_ri=1716403"},{"id":1180667,"name":"Optimality Condition","url":"https://www.academia.edu/Documents/in/Optimality_Condition?f_ri=1716403"},{"id":1255525,"name":"Chronic Liver Disease","url":"https://www.academia.edu/Documents/in/Chronic_Liver_Disease?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_73284106" data-work_id="73284106" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/73284106/Evaluation_of_a_Western_Blot_Method_for_the_Detection_of_Yersinia_Antibodies_Evidence_of_Serological_Cross_Reactivity_between_Yersinia_Outer_Membrane_Proteins_and_Borrelia_burgdorferi">Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Yersinia enterocolitica and Yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. We evaluated a Western blot assay which uses Yersinia outer membrane proteins as antigens for the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_73284106" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Yersinia enterocolitica and Yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. We evaluated a Western blot assay which uses Yersinia outer membrane proteins as antigens for the detection of Yersinia antibodies as a replacement for the complement fixation (CF) assay. Clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the CF assay, Western blot assay, and enzyme-linked immunosorbent assay (ELISA). The CF assay and ELISA were compared to the Western blot assay, which was the reference method used in this study. Sera with antibodies that could potentially cross-react with Yersinia were also tested by the Western blot assay. The agreement, sensitivity, and specificity of the CF method were 61%, 26%, and 95%, respectively; and those for the ELISA were 89%, 95%, and 82%, respectively. The prevalences of Yersinia antibodies in 50 healthy donors were 6% for immunoglobul...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/73284106" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="15e7c44fdecf43100869bd33b265401d" rel="nofollow" data-download="{"attachment_id":81863697,"asset_id":73284106,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/81863697/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="140511393" href="https://independent.academia.edu/Berkta%C5%9FMustafa">Mustafa Berktaş</a><script data-card-contents-for-user="140511393" type="text/json">{"id":140511393,"first_name":"Mustafa","last_name":"Berktaş","domain_name":"independent","page_name":"BerktaşMustafa","display_name":"Mustafa Berktaş","profile_url":"https://independent.academia.edu/Berkta%C5%9FMustafa?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_73284106 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="73284106"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 73284106, container: ".js-paper-rank-work_73284106", }); 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$(".js-view-count[data-work-id=73284106]").text(description); $(".js-view-count-work_73284106").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_73284106").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="73284106"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="34107" href="https://www.academia.edu/Documents/in/Chlamydia_pneumoniae">Chlamydia pneumoniae</a>, <script data-card-contents-for-ri="34107" type="text/json">{"id":34107,"name":"Chlamydia pneumoniae","url":"https://www.academia.edu/Documents/in/Chlamydia_pneumoniae?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="37834" href="https://www.academia.edu/Documents/in/Western_blotting">Western blotting</a>, <script data-card-contents-for-ri="37834" type="text/json">{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="98134" href="https://www.academia.edu/Documents/in/United_States">United States</a><script data-card-contents-for-ri="98134" type="text/json">{"id":98134,"name":"United States","url":"https://www.academia.edu/Documents/in/United_States?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=73284106]'), work: {"id":73284106,"title":"Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi","created_at":"2022-03-07T20:07:18.353-08:00","url":"https://www.academia.edu/73284106/Evaluation_of_a_Western_Blot_Method_for_the_Detection_of_Yersinia_Antibodies_Evidence_of_Serological_Cross_Reactivity_between_Yersinia_Outer_Membrane_Proteins_and_Borrelia_burgdorferi?f_ri=1716403","dom_id":"work_73284106","summary":"Yersinia enterocolitica and Yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. We evaluated a Western blot assay which uses Yersinia outer membrane proteins as antigens for the detection of Yersinia antibodies as a replacement for the complement fixation (CF) assay. Clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the CF assay, Western blot assay, and enzyme-linked immunosorbent assay (ELISA). The CF assay and ELISA were compared to the Western blot assay, which was the reference method used in this study. Sera with antibodies that could potentially cross-react with Yersinia were also tested by the Western blot assay. The agreement, sensitivity, and specificity of the CF method were 61%, 26%, and 95%, respectively; and those for the ELISA were 89%, 95%, and 82%, respectively. The prevalences of Yersinia antibodies in 50 healthy donors were 6% for immunoglobul...","downloadable_attachments":[{"id":81863697,"asset_id":73284106,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":140511393,"first_name":"Mustafa","last_name":"Berktaş","domain_name":"independent","page_name":"BerktaşMustafa","display_name":"Mustafa Berktaş","profile_url":"https://independent.academia.edu/Berkta%C5%9FMustafa?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":34107,"name":"Chlamydia pneumoniae","url":"https://www.academia.edu/Documents/in/Chlamydia_pneumoniae?f_ri=1716403","nofollow":false},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=1716403","nofollow":false},{"id":98134,"name":"United States","url":"https://www.academia.edu/Documents/in/United_States?f_ri=1716403","nofollow":false},{"id":128172,"name":"Borrelia burgdorferi","url":"https://www.academia.edu/Documents/in/Borrelia_burgdorferi?f_ri=1716403"},{"id":335965,"name":"Gram-negative bacteria","url":"https://www.academia.edu/Documents/in/Gram-negative_bacteria?f_ri=1716403"},{"id":435727,"name":"Major histocompatibility complex","url":"https://www.academia.edu/Documents/in/Major_histocompatibility_complex?f_ri=1716403"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot?f_ri=1716403"},{"id":537759,"name":"Gastrointestinal Tract","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Tract?f_ri=1716403"},{"id":538047,"name":"Young Children","url":"https://www.academia.edu/Documents/in/Young_Children?f_ri=1716403"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=1716403"},{"id":1180273,"name":"Outer Membrane Protein","url":"https://www.academia.edu/Documents/in/Outer_Membrane_Protein?f_ri=1716403"},{"id":1180715,"name":"Abdominal Pain","url":"https://www.academia.edu/Documents/in/Abdominal_Pain?f_ri=1716403"},{"id":1222799,"name":"Soil Water","url":"https://www.academia.edu/Documents/in/Soil_Water?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1995876,"name":"Yersinia Enterocolitica","url":"https://www.academia.edu/Documents/in/Yersinia_Enterocolitica?f_ri=1716403"},{"id":2056410,"name":"Complement fixation test","url":"https://www.academia.edu/Documents/in/Complement_fixation_test?f_ri=1716403"},{"id":3990429,"name":"Yersinia","url":"https://www.academia.edu/Documents/in/Yersinia?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_64209050" data-work_id="64209050" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/64209050/Apport_du_traitement_substitutif_par_les_immunoglobulines_au_cours_des_agammaglobulin%C3%A9mies_%C3%A0_propos_de_10_cas">Apport du traitement substitutif par les immunoglobulines au cours des agammaglobulinémies : à propos de 10 cas</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">L'administration par voie intraveineuse d'immunoglobulines polyvalentes (Ig IV) est utilisée, depuis de nombreuses années, dans le traitement substitutif des déficits immunitaires primitifs avec défaut de production d'anticorps. Dans... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_64209050" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">L'administration par voie intraveineuse d'immunoglobulines polyvalentes (Ig IV) est utilisée, depuis de nombreuses années, dans le traitement substitutif des déficits immunitaires primitifs avec défaut de production d'anticorps. Dans cette étude, nous avons évalué le devenir d'enfants atteints par ces maladies ayant reçu ce type de traitement de façon prolongée. Patients et méthodes.-Il s'agit d'une étude rétrospective évaluant les manifestations cliniques avant puis pendant le traitement par les Ig IV en fonction du taux des IgG résiduelles chez 10 enfants atteints d'agammaglobulinémie, dont 9 garçons et 1 fille. Le taux des Ig sériques a été dosé par néphélémétrie. Les lymphocytes B circulants, les lymphocytes T et les sous-populations lymphocytaires T ont été comptés en utilisant des anticorps monoclonaux. La fonction des lymphocytes T a été évaluée en utilisant des tests de prolifération induits par les mitogènes (phytohémagglutinine) et les antigènes (tuberculine, anatoxine tétanique). À chaque séance de traitement par Ig IV, l'état clinique des patients était évalué, les épisodes infectieux et le traitement antibiotique reçu notés dans le dossier médical et un prélèvement bactériologique effectué au niveau du point d'appel en cas d'infection. Résultats.-Le nombre cumulatif des mois de traitement était de 866 mois avec une durée médiane du traitement de 108 mois. Ce traitement a été systématiquement administré en moyenne 1 fois toutes les 4 semaines. La médiane des doses administrées était de 500 mg/kg par mois (300-900 mg/kg/mois). Durant le traitement par les Ig IV, les taux moyens d'IgG résiduelles par patient variaient de 2,4 à 6,2 g/l (moyenne = 3,9 g/l). Avant l'administration d'Ig IV, les patients avaient eu un total de 99 infections bactériennes à prédominance respiratoire (48,5 %). Quatre patients avaient au moment de l'instauration du traitement une dilatation des bronches. Le nombre global d'infections a été réduit à 25 pendant le traitement par les Ig IV, et le taux d'infection par patient et par année a chuté de façon significative. Un patient a eu une méningoencéphalite à Echovirus 27 durant le traitement. Conclusion.-Des taux moyens d'IgG résiduelles de 3,9 g/l ont permis de réduire la fréquence des infections bactériennes de façon significative. Le traitement des patients par Ig IV, leur mise sous antibiothérapie spécifique et les séances de kinésithérapie respiratoire ont permis une réduction importante des infections respiratoires, et la stabilisation des lésions de dilatation des bronches. En revanche, ce traitement ne protège pas des méningoencéphalites virales.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/64209050" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="661f0315e8bb85626e6992a5bc95d5ef" rel="nofollow" data-download="{"attachment_id":76349736,"asset_id":64209050,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/76349736/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="52094736" href="https://independent.academia.edu/FethiMellouli">Fethi Mellouli</a><script data-card-contents-for-user="52094736" type="text/json">{"id":52094736,"first_name":"Fethi","last_name":"Mellouli","domain_name":"independent","page_name":"FethiMellouli","display_name":"Fethi Mellouli","profile_url":"https://independent.academia.edu/FethiMellouli?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_64209050 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="64209050"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 64209050, container: ".js-paper-rank-work_64209050", }); 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$(".js-view-count[data-work-id=64209050]").text(description); $(".js-view-count-work_64209050").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_64209050").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="64209050"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="134346" href="https://www.academia.edu/Documents/in/Infant">Infant</a>, <script data-card-contents-for-ri="134346" type="text/json">{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="469105" href="https://www.academia.edu/Documents/in/Retrospective_Studies">Retrospective Studies</a>, <script data-card-contents-for-ri="469105" type="text/json">{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="587528" href="https://www.academia.edu/Documents/in/Bacterial_infections">Bacterial infections</a><script data-card-contents-for-ri="587528" type="text/json">{"id":587528,"name":"Bacterial infections","url":"https://www.academia.edu/Documents/in/Bacterial_infections?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=64209050]'), work: {"id":64209050,"title":"Apport du traitement substitutif par les immunoglobulines au cours des agammaglobulinémies : à propos de 10 cas","created_at":"2021-12-15T00:09:09.611-08:00","url":"https://www.academia.edu/64209050/Apport_du_traitement_substitutif_par_les_immunoglobulines_au_cours_des_agammaglobulin%C3%A9mies_%C3%A0_propos_de_10_cas?f_ri=1716403","dom_id":"work_64209050","summary":"L'administration par voie intraveineuse d'immunoglobulines polyvalentes (Ig IV) est utilisée, depuis de nombreuses années, dans le traitement substitutif des déficits immunitaires primitifs avec défaut de production d'anticorps. Dans cette étude, nous avons évalué le devenir d'enfants atteints par ces maladies ayant reçu ce type de traitement de façon prolongée. Patients et méthodes.-Il s'agit d'une étude rétrospective évaluant les manifestations cliniques avant puis pendant le traitement par les Ig IV en fonction du taux des IgG résiduelles chez 10 enfants atteints d'agammaglobulinémie, dont 9 garçons et 1 fille. Le taux des Ig sériques a été dosé par néphélémétrie. Les lymphocytes B circulants, les lymphocytes T et les sous-populations lymphocytaires T ont été comptés en utilisant des anticorps monoclonaux. La fonction des lymphocytes T a été évaluée en utilisant des tests de prolifération induits par les mitogènes (phytohémagglutinine) et les antigènes (tuberculine, anatoxine tétanique). À chaque séance de traitement par Ig IV, l'état clinique des patients était évalué, les épisodes infectieux et le traitement antibiotique reçu notés dans le dossier médical et un prélèvement bactériologique effectué au niveau du point d'appel en cas d'infection. Résultats.-Le nombre cumulatif des mois de traitement était de 866 mois avec une durée médiane du traitement de 108 mois. Ce traitement a été systématiquement administré en moyenne 1 fois toutes les 4 semaines. La médiane des doses administrées était de 500 mg/kg par mois (300-900 mg/kg/mois). Durant le traitement par les Ig IV, les taux moyens d'IgG résiduelles par patient variaient de 2,4 à 6,2 g/l (moyenne = 3,9 g/l). Avant l'administration d'Ig IV, les patients avaient eu un total de 99 infections bactériennes à prédominance respiratoire (48,5 %). Quatre patients avaient au moment de l'instauration du traitement une dilatation des bronches. Le nombre global d'infections a été réduit à 25 pendant le traitement par les Ig IV, et le taux d'infection par patient et par année a chuté de façon significative. Un patient a eu une méningoencéphalite à Echovirus 27 durant le traitement. Conclusion.-Des taux moyens d'IgG résiduelles de 3,9 g/l ont permis de réduire la fréquence des infections bactériennes de façon significative. Le traitement des patients par Ig IV, leur mise sous antibiothérapie spécifique et les séances de kinésithérapie respiratoire ont permis une réduction importante des infections respiratoires, et la stabilisation des lésions de dilatation des bronches. En revanche, ce traitement ne protège pas des méningoencéphalites virales.","downloadable_attachments":[{"id":76349736,"asset_id":64209050,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":52094736,"first_name":"Fethi","last_name":"Mellouli","domain_name":"independent","page_name":"FethiMellouli","display_name":"Fethi Mellouli","profile_url":"https://independent.academia.edu/FethiMellouli?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false},{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=1716403","nofollow":false},{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=1716403","nofollow":false},{"id":587528,"name":"Bacterial infections","url":"https://www.academia.edu/Documents/in/Bacterial_infections?f_ri=1716403","nofollow":false},{"id":990198,"name":"Retrospective Study","url":"https://www.academia.edu/Documents/in/Retrospective_Study?f_ri=1716403"},{"id":1426712,"name":"Immunoglobulin","url":"https://www.academia.edu/Documents/in/Immunoglobulin?f_ri=1716403"},{"id":1489223,"name":"Intravenous Immunoglobulin","url":"https://www.academia.edu/Documents/in/Intravenous_Immunoglobulin?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1917237,"name":"Agammaglobulinemia","url":"https://www.academia.edu/Documents/in/Agammaglobulinemia?f_ri=1716403"},{"id":2489700,"name":"Child preschool","url":"https://www.academia.edu/Documents/in/Child_preschool?f_ri=1716403"},{"id":3789883,"name":"Paediatrics and reproductive medicine","url":"https://www.academia.edu/Documents/in/Paediatrics_and_reproductive_medicine?f_ri=1716403"}]}, }) 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patients with lymphoid malignancies: influence of antibody isotype</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The CAMPATH-1 family of antibodies recognize an abundant glycoprotein expressed on virtually all human lymphocytes. All rat IgM and IgG antibodies of this specificity are lytic with human complement, but only IgG2b is active in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_51674755" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The CAMPATH-1 family of antibodies recognize an abundant glycoprotein expressed on virtually all human lymphocytes. All rat IgM and IgG antibodies of this specificity are lytic with human complement, but only IgG2b is active in antibody-dependent cell-mediated cytotoxicity (ADCC). We compared the ability of IgM, IgG2a, and IgG2b to deplete lymphocytes in vivo in two patients with prolymphocytic transformation of B-cell chronic lymphocytic leukemia (CLL). The IgM (CAMPATH-1M) produced transient depletion of blood lymphocytes with consumption of complement but had no effect on solid masses or bone marrow. Similar transient depletion of blood lymphocytes was noted with the IgG2a (YTH34.5). In contrast, the IgG2b (CAMPATH-1G) produced long-lasting depletion of lymphocytes from blood and marrow and improvement in splenomegaly but no detectable changes in complement levels. These differences probably reflect the importance of Fc receptor binding for effective clearance of target cells in ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/51674755" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="357d1d8f30fa1cb23999b4a9bb0bdd04" rel="nofollow" data-download="{"attachment_id":69294621,"asset_id":51674755,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/69294621/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="55958776" href="https://independent.academia.edu/MartinDyer">Martin Dyer</a><script data-card-contents-for-user="55958776" type="text/json">{"id":55958776,"first_name":"Martin","last_name":"Dyer","domain_name":"independent","page_name":"MartinDyer","display_name":"Martin Dyer","profile_url":"https://independent.academia.edu/MartinDyer?f_ri=1716403","photo":"https://0.academia-photos.com/55958776/20163095/19903372/s65_martin.dyer.jpg"}</script></span></span></li><li class="js-paper-rank-work_51674755 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="51674755"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 51674755, container: ".js-paper-rank-work_51674755", }); 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All rat IgM and IgG antibodies of this specificity are lytic with human complement, but only IgG2b is active in antibody-dependent cell-mediated cytotoxicity (ADCC). We compared the ability of IgM, IgG2a, and IgG2b to deplete lymphocytes in vivo in two patients with prolymphocytic transformation of B-cell chronic lymphocytic leukemia (CLL). The IgM (CAMPATH-1M) produced transient depletion of blood lymphocytes with consumption of complement but had no effect on solid masses or bone marrow. Similar transient depletion of blood lymphocytes was noted with the IgG2a (YTH34.5). In contrast, the IgG2b (CAMPATH-1G) produced long-lasting depletion of lymphocytes from blood and marrow and improvement in splenomegaly but no detectable changes in complement levels. These differences probably reflect the importance of Fc receptor binding for effective clearance of target cells in ...","downloadable_attachments":[{"id":69294621,"asset_id":51674755,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":55958776,"first_name":"Martin","last_name":"Dyer","domain_name":"independent","page_name":"MartinDyer","display_name":"Martin Dyer","profile_url":"https://independent.academia.edu/MartinDyer?f_ri=1716403","photo":"https://0.academia-photos.com/55958776/20163095/19903372/s65_martin.dyer.jpg"}],"research_interests":[{"id":100732,"name":"Blood","url":"https://www.academia.edu/Documents/in/Blood?f_ri=1716403","nofollow":false},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403","nofollow":false},{"id":244814,"name":"Clinical 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imunológicos</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">O objetivo do estudo foi examinar a influência de dois diferentes protocolos de treinamento de força sobre parâmetros antropométricos (peso, IMC, massa gorda), funcionais (teste de 1-RM e teste de repetições máximas) e relacionados ao... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_41675929" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">O objetivo do estudo foi examinar a influência de dois diferentes protocolos de treinamento de força sobre parâmetros antropométricos (peso, IMC, massa gorda), funcionais (teste de 1-RM e teste de repetições máximas) e relacionados ao sistema endócrino (concentração de testosterona e de cortisol) e ao sistema imunológico (concentração de glutamina e de IgG). Participaram do estudo 12 homens treinados (27,4 ± 4,8 anos). Esses indivíduos foram aleatoriamente divididos em dois grupos, que posteriormente foram submetidos a dois protocolos de treinamento distintos: Múltiplas séries (MS) e Tri-set (TS). Amostras de sangue foram coletadas antes e depois de uma sessão de exercício de força, no início e no final do período de oito semanas de treinamento. Não foram observadas alterações nos parâmetros morfo-funcionais (com exceção do teste de repetições máximas para o agachamento). Com relação aos parâmetros endócrinos, foi observado que o TS provocou aumento significativo do cortisol, imedia...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/41675929" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="13d382f9970c7e94babb32c0c4a5074e" rel="nofollow" data-download="{"attachment_id":61831186,"asset_id":41675929,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/61831186/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="142599739" href="https://independent.academia.edu/FrankBacurau">Frank Bacurau</a><script data-card-contents-for-user="142599739" type="text/json">{"id":142599739,"first_name":"Frank","last_name":"Bacurau","domain_name":"independent","page_name":"FrankBacurau","display_name":"Frank Bacurau","profile_url":"https://independent.academia.edu/FrankBacurau?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_41675929 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="41675929"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 41675929, container: ".js-paper-rank-work_41675929", }); 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Participaram do estudo 12 homens treinados (27,4 ± 4,8 anos). Esses indivíduos foram aleatoriamente divididos em dois grupos, que posteriormente foram submetidos a dois protocolos de treinamento distintos: Múltiplas séries (MS) e Tri-set (TS). Amostras de sangue foram coletadas antes e depois de uma sessão de exercício de força, no início e no final do período de oito semanas de treinamento. Não foram observadas alterações nos parâmetros morfo-funcionais (com exceção do teste de repetições máximas para o agachamento). Com relação aos parâmetros endócrinos, foi observado que o TS provocou aumento significativo do cortisol, imedia...","downloadable_attachments":[{"id":61831186,"asset_id":41675929,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":142599739,"first_name":"Frank","last_name":"Bacurau","domain_name":"independent","page_name":"FrankBacurau","display_name":"Frank Bacurau","profile_url":"https://independent.academia.edu/FrankBacurau?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":58431,"name":"Cortisol","url":"https://www.academia.edu/Documents/in/Cortisol?f_ri=1716403","nofollow":false},{"id":103298,"name":"Blood sampling","url":"https://www.academia.edu/Documents/in/Blood_sampling?f_ri=1716403","nofollow":false},{"id":112275,"name":"Resistance Training","url":"https://www.academia.edu/Documents/in/Resistance_Training?f_ri=1716403","nofollow":false},{"id":353163,"name":"Fat Mass","url":"https://www.academia.edu/Documents/in/Fat_Mass?f_ri=1716403","nofollow":false},{"id":1529398,"name":"Palabras Clave: BIM","url":"https://www.academia.edu/Documents/in/Palabras_Clave_BIM?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_30038171" data-work_id="30038171" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/30038171/Bidirectional_FCRN_Dependent_Igg_Transport_in_a_Polarized_Human_Intestinalepithelial_Cell_Line">Bidirectional FCRN-Dependent Igg Transport in a Polarized Human Intestinalepithelial Cell Line</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Steady-state levels of IgG in the blood of adult mice, and likely all mammals, depend on IgG catabolism mediated in part by the MHC class I-related Fc receptor, FcRn (1). FcRn also mediates vectorial transport of IgG across certain... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_30038171" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Steady-state levels of IgG in the blood of adult mice, and likely all mammals, depend on IgG catabolism mediated in part by the MHC class I-related Fc receptor, FcRn (1). FcRn also mediates vectorial transport of IgG across certain epithelial barriers. In suckling mice and rats, intestinal absorption of maternal IgG from breast milk into the systemic circulation depends on FcRn (2). In humans, maternofetal transfer of IgG across the placenta also likely depends on FcRn (3). Thus, FcRn plays critical and well-documented roles in the regulation of IgG metabolism in adults and in the acquisition of humoral immunity in early life. These effects on the physiology of IgG in vivo result from the action of FcRn as an intracellular trafficking receptor (4).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/30038171" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1d238601daa6c43bdf56c599c5488e54" rel="nofollow" data-download="{"attachment_id":50490430,"asset_id":30038171,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50490430/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38620949" href="https://independent.academia.edu/JeremyAhouse">Jeremy Ahouse</a><script data-card-contents-for-user="38620949" type="text/json">{"id":38620949,"first_name":"Jeremy","last_name":"Ahouse","domain_name":"independent","page_name":"JeremyAhouse","display_name":"Jeremy Ahouse","profile_url":"https://independent.academia.edu/JeremyAhouse?f_ri=1716403","photo":"https://0.academia-photos.com/38620949/11335391/12645463/s65_jeremy.ahouse.jpg"}</script></span></span></li><li class="js-paper-rank-work_30038171 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="30038171"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 30038171, container: ".js-paper-rank-work_30038171", }); 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$(".js-view-count[data-work-id=30038171]").text(description); $(".js-view-count-work_30038171").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_30038171").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="30038171"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="57808" href="https://www.academia.edu/Documents/in/Cell_line">Cell line</a>, <script data-card-contents-for-ri="57808" type="text/json">{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62550" href="https://www.academia.edu/Documents/in/Pregnancy">Pregnancy</a>, <script data-card-contents-for-ri="62550" type="text/json">{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71471" href="https://www.academia.edu/Documents/in/Intestinal_Mucosa">Intestinal Mucosa</a>, <script data-card-contents-for-ri="71471" type="text/json">{"id":71471,"name":"Intestinal Mucosa","url":"https://www.academia.edu/Documents/in/Intestinal_Mucosa?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a><script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=30038171]'), work: {"id":30038171,"title":"Bidirectional FCRN-Dependent Igg Transport in a Polarized Human Intestinalepithelial Cell Line","created_at":"2016-11-22T18:01:13.341-08:00","url":"https://www.academia.edu/30038171/Bidirectional_FCRN_Dependent_Igg_Transport_in_a_Polarized_Human_Intestinalepithelial_Cell_Line?f_ri=1716403","dom_id":"work_30038171","summary":"Steady-state levels of IgG in the blood of adult mice, and likely all mammals, depend on IgG catabolism mediated in part by the MHC class I-related Fc receptor, FcRn (1). FcRn also mediates vectorial transport of IgG across certain epithelial barriers. In suckling mice and rats, intestinal absorption of maternal IgG from breast milk into the systemic circulation depends on FcRn (2). In humans, maternofetal transfer of IgG across the placenta also likely depends on FcRn (3). Thus, FcRn plays critical and well-documented roles in the regulation of IgG metabolism in adults and in the acquisition of humoral immunity in early life. These effects on the physiology of IgG in vivo result from the action of FcRn as an intracellular trafficking receptor (4).","downloadable_attachments":[{"id":50490430,"asset_id":30038171,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38620949,"first_name":"Jeremy","last_name":"Ahouse","domain_name":"independent","page_name":"JeremyAhouse","display_name":"Jeremy Ahouse","profile_url":"https://independent.academia.edu/JeremyAhouse?f_ri=1716403","photo":"https://0.academia-photos.com/38620949/11335391/12645463/s65_jeremy.ahouse.jpg"}],"research_interests":[{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=1716403","nofollow":false},{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=1716403","nofollow":false},{"id":71471,"name":"Intestinal Mucosa","url":"https://www.academia.edu/Documents/in/Intestinal_Mucosa?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical?f_ri=1716403"},{"id":122569,"name":"Cell Polarity","url":"https://www.academia.edu/Documents/in/Cell_Polarity?f_ri=1716403"},{"id":288383,"name":"Intestinal absorption","url":"https://www.academia.edu/Documents/in/Intestinal_absorption?f_ri=1716403"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=1716403"},{"id":1144154,"name":"Clinical Investigation","url":"https://www.academia.edu/Documents/in/Clinical_Investigation?f_ri=1716403"},{"id":1653420,"name":"Maternal-fetal exchange","url":"https://www.academia.edu/Documents/in/Maternal-fetal_exchange?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2468093,"name":"Cell Membrane","url":"https://www.academia.edu/Documents/in/Cell_Membrane?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10863275" data-work_id="10863275" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10863275/Serum_immunoglobulins_in_non_Hodgkins_lymphoma_patients">Serum immunoglobulins in non-Hodgkin's lymphoma patients</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Out of them 96 (81%) patients had B non-Hodgkin lymphoma (B-NHL), and 23 (19%) T-NHL. Indolent type of NHL was more frequent (77 patients, 65%), then aggressive type of NHL (42 patients, 35%). Most patients had normal serum protein... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_10863275" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Out of them 96 (81%) patients had B non-Hodgkin lymphoma (B-NHL), and 23 (19%) T-NHL. Indolent type of NHL was more frequent (77 patients, 65%), then aggressive type of NHL (42 patients, 35%). Most patients had normal serum protein concentration, the increased protein concentration was seen in 17% of patients while decreased concentration was noticed in 7% of patients. Hypoalbuminaemia was more frequent (43%) then hyperalbuminaemia (1%). In contrast to albumin, low levels of other protein fractions (alpha1-, alpha2-, and beta-globulin) were rather rare (0.6%, 4%, and 3% of patients, respectively) and high levels were frequent (23%, 37%, and 8%, respectively). Polyclonal hyperimmunoglobulinaemia was more frequent finding than hypoimmunoglobulinaemia. In 29% patients higher IgG level and in 25% patients higher IgA level were found. IgM hypoimmunoglobulinaemia (22%) was more frequent than IgG (11%) and IgA (8%) hypoimmunoglobulinaemia. M-spike in serum protein electrophoresis was found in 11 (7%) patients. The statistically significant association was not found between serum Ig concentration and lymphoma malignancy grade as well as between serum Ig concentration and immunologic origin of lymphoma. T-NHL patients have more often IgA concentration level above or under normal values than B-NHL patients (p<0.05).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10863275" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d69cc39539dcd55e25a89b6e475eaee8" rel="nofollow" data-download="{"attachment_id":47068129,"asset_id":10863275,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47068129/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="26388638" href="https://independent.academia.edu/BJaksic">Branimir Jaksic</a><script data-card-contents-for-user="26388638" type="text/json">{"id":26388638,"first_name":"Branimir","last_name":"Jaksic","domain_name":"independent","page_name":"BJaksic","display_name":"Branimir Jaksic","profile_url":"https://independent.academia.edu/BJaksic?f_ri=1716403","photo":"https://0.academia-photos.com/26388638/119838079/109159283/s65_branimir.jaksic.jpeg"}</script></span></span></li><li class="js-paper-rank-work_10863275 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10863275"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10863275, container: ".js-paper-rank-work_10863275", }); 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$(".js-view-count[data-work-id=10863275]").text(description); $(".js-view-count-work_10863275").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_10863275").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="10863275"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="767" href="https://www.academia.edu/Documents/in/Anthropology">Anthropology</a>, <script data-card-contents-for-ri="767" type="text/json">{"id":767,"name":"Anthropology","url":"https://www.academia.edu/Documents/in/Anthropology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19753" href="https://www.academia.edu/Documents/in/Serum_Proteins">Serum Proteins</a>, <script data-card-contents-for-ri="19753" type="text/json">{"id":19753,"name":"Serum Proteins","url":"https://www.academia.edu/Documents/in/Serum_Proteins?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="469105" href="https://www.academia.edu/Documents/in/Retrospective_Studies">Retrospective Studies</a>, <script data-card-contents-for-ri="469105" type="text/json">{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1312086" href="https://www.academia.edu/Documents/in/Immunoglobulins">Immunoglobulins</a><script data-card-contents-for-ri="1312086" type="text/json">{"id":1312086,"name":"Immunoglobulins","url":"https://www.academia.edu/Documents/in/Immunoglobulins?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=10863275]'), work: {"id":10863275,"title":"Serum immunoglobulins in non-Hodgkin's lymphoma patients","created_at":"2015-02-17T03:39:51.540-08:00","url":"https://www.academia.edu/10863275/Serum_immunoglobulins_in_non_Hodgkins_lymphoma_patients?f_ri=1716403","dom_id":"work_10863275","summary":"Out of them 96 (81%) patients had B non-Hodgkin lymphoma (B-NHL), and 23 (19%) T-NHL. Indolent type of NHL was more frequent (77 patients, 65%), then aggressive type of NHL (42 patients, 35%). Most patients had normal serum protein concentration, the increased protein concentration was seen in 17% of patients while decreased concentration was noticed in 7% of patients. Hypoalbuminaemia was more frequent (43%) then hyperalbuminaemia (1%). In contrast to albumin, low levels of other protein fractions (alpha1-, alpha2-, and beta-globulin) were rather rare (0.6%, 4%, and 3% of patients, respectively) and high levels were frequent (23%, 37%, and 8%, respectively). Polyclonal hyperimmunoglobulinaemia was more frequent finding than hypoimmunoglobulinaemia. In 29% patients higher IgG level and in 25% patients higher IgA level were found. IgM hypoimmunoglobulinaemia (22%) was more frequent than IgG (11%) and IgA (8%) hypoimmunoglobulinaemia. M-spike in serum protein electrophoresis was found in 11 (7%) patients. The statistically significant association was not found between serum Ig concentration and lymphoma malignancy grade as well as between serum Ig concentration and immunologic origin of lymphoma. T-NHL patients have more often IgA concentration level above or under normal values than B-NHL patients (p\u003c0.05).","downloadable_attachments":[{"id":47068129,"asset_id":10863275,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":26388638,"first_name":"Branimir","last_name":"Jaksic","domain_name":"independent","page_name":"BJaksic","display_name":"Branimir Jaksic","profile_url":"https://independent.academia.edu/BJaksic?f_ri=1716403","photo":"https://0.academia-photos.com/26388638/119838079/109159283/s65_branimir.jaksic.jpeg"}],"research_interests":[{"id":767,"name":"Anthropology","url":"https://www.academia.edu/Documents/in/Anthropology?f_ri=1716403","nofollow":false},{"id":19753,"name":"Serum Proteins","url":"https://www.academia.edu/Documents/in/Serum_Proteins?f_ri=1716403","nofollow":false},{"id":469105,"name":"Retrospective 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u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13093997/Clinicopathological_definition_of_Waldenstroms_macroglobulinemia_Consensus_Panel_Recommendations_from_the_Second_International_Workshop_on_Waldenstroms_Macroglobulinemia">Clinicopathological definition of Waldenstrom's macroglobulinemia: Consensus Panel Recommendations from the Second International Workshop on Waldenstrom's Macroglobulinemia</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13093997" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a 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class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13093997; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_13093997"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13093997 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13093997"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13093997; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13093997]").text(description); $(".js-view-count-work_13093997").attr('title', 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type="text/json">{"id":50991,"name":"Cytogenetics","url":"https://www.academia.edu/Documents/in/Cytogenetics?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="55269" href="https://www.academia.edu/Documents/in/Lymphoma">Lymphoma</a>, <script data-card-contents-for-ri="55269" type="text/json">{"id":55269,"name":"Lymphoma","url":"https://www.academia.edu/Documents/in/Lymphoma?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="159239" href="https://www.academia.edu/Documents/in/Bone_marrow">Bone marrow</a><script data-card-contents-for-ri="159239" type="text/json">{"id":159239,"name":"Bone marrow","url":"https://www.academia.edu/Documents/in/Bone_marrow?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13093997]'), work: {"id":13093997,"title":"Clinicopathological definition of Waldenstrom's macroglobulinemia: Consensus Panel Recommendations from the Second International Workshop on Waldenstrom's Macroglobulinemia","created_at":"2015-06-19T00:25:23.227-07:00","url":"https://www.academia.edu/13093997/Clinicopathological_definition_of_Waldenstroms_macroglobulinemia_Consensus_Panel_Recommendations_from_the_Second_International_Workshop_on_Waldenstroms_Macroglobulinemia?f_ri=1716403","dom_id":"work_13093997","summary":null,"downloadable_attachments":[{"id":45674191,"asset_id":13093997,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32343055,"first_name":"Jesús","last_name":"Miguel","domain_name":"independent","page_name":"JesúsMiguel5","display_name":"Jesús Miguel","profile_url":"https://independent.academia.edu/Jes%C3%BAsMiguel5?f_ri=1716403","photo":"https://0.academia-photos.com/32343055/9684916/10786568/s65_jes_s.miguel.jpg"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=1716403","nofollow":false},{"id":50991,"name":"Cytogenetics","url":"https://www.academia.edu/Documents/in/Cytogenetics?f_ri=1716403","nofollow":false},{"id":55269,"name":"Lymphoma","url":"https://www.academia.edu/Documents/in/Lymphoma?f_ri=1716403","nofollow":false},{"id":159239,"name":"Bone marrow","url":"https://www.academia.edu/Documents/in/Bone_marrow?f_ri=1716403","nofollow":false},{"id":179800,"name":"Definition","url":"https://www.academia.edu/Documents/in/Definition?f_ri=1716403"},{"id":341842,"name":"Seminars","url":"https://www.academia.edu/Documents/in/Seminars?f_ri=1716403"},{"id":521881,"name":"World Health Organization","url":"https://www.academia.edu/Documents/in/World_Health_Organization?f_ri=1716403"},{"id":571886,"name":"Immunophenotyping","url":"https://www.academia.edu/Documents/in/Immunophenotyping?f_ri=1716403"},{"id":1198608,"name":"Immunophenotype","url":"https://www.academia.edu/Documents/in/Immunophenotype?f_ri=1716403"},{"id":1312086,"name":"Immunoglobulins","url":"https://www.academia.edu/Documents/in/Immunoglobulins?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24943333 coauthored" data-work_id="24943333" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24943333/Homologous_and_Heterologous_Immune_Reactions_between_Venezuelan_Geographic_Isolates_of_Anaplasma_marginale">Homologous and Heterologous Immune Reactions between Venezuelan Geographic Isolates of Anaplasma marginale</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24943333" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9c7c075aeffb4e9d7798ae5553c49c01" rel="nofollow" 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type="text/json">{"id":277709000,"first_name":"Alfredo","last_name":"Coronado","domain_name":"independent","page_name":"AlfredoCoronado29","display_name":"Alfredo Coronado","profile_url":"https://independent.academia.edu/AlfredoCoronado29?f_ri=1716403","photo":"https://0.academia-photos.com/277709000/126582117/115958492/s65_alfredo.coronado.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-24943333">+1</span><div class="hidden js-additional-users-24943333"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://usb.academia.edu/PedroAso">Pedro Aso</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-24943333'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-24943333').html(); } } new 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container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24943333 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24943333"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24943333; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24943333]").text(description); $(".js-view-count-work_24943333").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24943333").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24943333"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="261" href="https://www.academia.edu/Documents/in/Geography">Geography</a>, <script data-card-contents-for-ri="261" type="text/json">{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="11969" href="https://www.academia.edu/Documents/in/Venezuela">Venezuela</a>, <script data-card-contents-for-ri="11969" type="text/json">{"id":11969,"name":"Venezuela","url":"https://www.academia.edu/Documents/in/Venezuela?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="260829" href="https://www.academia.edu/Documents/in/Cattle">Cattle</a><script data-card-contents-for-ri="260829" type="text/json">{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24943333]'), work: {"id":24943333,"title":"Homologous and Heterologous Immune Reactions between Venezuelan Geographic Isolates of Anaplasma marginale","created_at":"2016-05-02T05:36:20.218-07:00","url":"https://www.academia.edu/24943333/Homologous_and_Heterologous_Immune_Reactions_between_Venezuelan_Geographic_Isolates_of_Anaplasma_marginale?f_ri=1716403","dom_id":"work_24943333","summary":null,"downloadable_attachments":[{"id":45274929,"asset_id":24943333,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":277709000,"first_name":"Alfredo","last_name":"Coronado","domain_name":"independent","page_name":"AlfredoCoronado29","display_name":"Alfredo Coronado","profile_url":"https://independent.academia.edu/AlfredoCoronado29?f_ri=1716403","photo":"https://0.academia-photos.com/277709000/126582117/115958492/s65_alfredo.coronado.png"},{"id":48090379,"first_name":"Pedro","last_name":"Aso","domain_name":"usb","page_name":"PedroAso","display_name":"Pedro Aso","profile_url":"https://usb.academia.edu/PedroAso?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography?f_ri=1716403","nofollow":false},{"id":11969,"name":"Venezuela","url":"https://www.academia.edu/Documents/in/Venezuela?f_ri=1716403","nofollow":false},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1716403","nofollow":false},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=1716403","nofollow":false},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=1716403"},{"id":521909,"name":"Anaplasmosis","url":"https://www.academia.edu/Documents/in/Anaplasmosis?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13753101" data-work_id="13753101" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13753101/Correlation_of_clinical_magnetic_resonance_imaging_and_cerebrospinal_fluid_findings_in_optic_neuritis">Correlation of clinical, magnetic resonance imaging, and cerebrospinal fluid findings in optic neuritis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We found 42 of 7 4 patients (57%) with isolated monosymptomatic optic neuritis to have 1 to 20 brain lesions, by magnetic resonance imaging (MRI). All of the brain lesions were clinically silent and had characteristics consistent with... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13753101" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We found 42 of 7 4 patients (57%) with isolated monosymptomatic optic neuritis to have 1 to 20 brain lesions, by magnetic resonance imaging (MRI). All of the brain lesions were clinically silent and had characteristics consistent with multiple sclerosis (MS). None of the patients had ever experienced neurologic symptoms prior to the episode of optic neuritis. During 5.6 years of follow-up, 21 patients (28%) developed definite MS on clinical grounds. Sixteen of the 21 converting patients (76%) had abnormal MRIs; the other 5 (24%) had MRIs that were normal initially (when they had optic neuritis only) and when repeated after they had developed clinical MS in 4 of the 5. Of the 53 patients who have not developed clinically definite MS, 26 (49%) have abnormal MRIs and 27 (51%) have normal MRIs. The finding of an abnormal MRI at the time of optic neuritis was significantly related to the subsequent development of MS on clinical grounds, but interpretation of the strength of that relationship must be tempered by the fact that some of the converting patients had normal MFUs and approximately half of the patients who did not develop clinical MS had abnormal MRIs.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13753101" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="055e8f216851c61ed38b9cef0cc7a6cf" rel="nofollow" data-download="{"attachment_id":44992455,"asset_id":13753101,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44992455/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32871407" href="https://spanalumni.academia.edu/LawrenceJacobs">Lawrence Jacobs</a><script data-card-contents-for-user="32871407" type="text/json">{"id":32871407,"first_name":"Lawrence","last_name":"Jacobs","domain_name":"spanalumni","page_name":"LawrenceJacobs","display_name":"Lawrence Jacobs","profile_url":"https://spanalumni.academia.edu/LawrenceJacobs?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13753101 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13753101"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13753101, container: ".js-paper-rank-work_13753101", }); });</script></li><li class="js-percentile-work_13753101 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13753101; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_13753101"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13753101 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13753101"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13753101; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13753101]").text(description); $(".js-view-count-work_13753101").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13753101").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13753101"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3097" href="https://www.academia.edu/Documents/in/Multiple_sclerosis">Multiple sclerosis</a>, <script data-card-contents-for-ri="3097" type="text/json">{"id":3097,"name":"Multiple sclerosis","url":"https://www.academia.edu/Documents/in/Multiple_sclerosis?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6200" href="https://www.academia.edu/Documents/in/Magnetic_Resonance_Imaging">Magnetic Resonance Imaging</a>, <script data-card-contents-for-ri="6200" type="text/json">{"id":6200,"name":"Magnetic Resonance Imaging","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Imaging?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a>, <script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="60971" href="https://www.academia.edu/Documents/in/Cerebrospinal_Fluid">Cerebrospinal Fluid</a><script data-card-contents-for-ri="60971" type="text/json">{"id":60971,"name":"Cerebrospinal Fluid","url":"https://www.academia.edu/Documents/in/Cerebrospinal_Fluid?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13753101]'), work: {"id":13753101,"title":"Correlation of clinical, magnetic resonance imaging, and cerebrospinal fluid findings in optic neuritis","created_at":"2015-07-07T08:55:54.862-07:00","url":"https://www.academia.edu/13753101/Correlation_of_clinical_magnetic_resonance_imaging_and_cerebrospinal_fluid_findings_in_optic_neuritis?f_ri=1716403","dom_id":"work_13753101","summary":"We found 42 of 7 4 patients (57%) with isolated monosymptomatic optic neuritis to have 1 to 20 brain lesions, by magnetic resonance imaging (MRI). All of the brain lesions were clinically silent and had characteristics consistent with multiple sclerosis (MS). None of the patients had ever experienced neurologic symptoms prior to the episode of optic neuritis. During 5.6 years of follow-up, 21 patients (28%) developed definite MS on clinical grounds. Sixteen of the 21 converting patients (76%) had abnormal MRIs; the other 5 (24%) had MRIs that were normal initially (when they had optic neuritis only) and when repeated after they had developed clinical MS in 4 of the 5. Of the 53 patients who have not developed clinically definite MS, 26 (49%) have abnormal MRIs and 27 (51%) have normal MRIs. The finding of an abnormal MRI at the time of optic neuritis was significantly related to the subsequent development of MS on clinical grounds, but interpretation of the strength of that relationship must be tempered by the fact that some of the converting patients had normal MFUs and approximately half of the patients who did not develop clinical MS had abnormal MRIs.","downloadable_attachments":[{"id":44992455,"asset_id":13753101,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32871407,"first_name":"Lawrence","last_name":"Jacobs","domain_name":"spanalumni","page_name":"LawrenceJacobs","display_name":"Lawrence Jacobs","profile_url":"https://spanalumni.academia.edu/LawrenceJacobs?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3097,"name":"Multiple sclerosis","url":"https://www.academia.edu/Documents/in/Multiple_sclerosis?f_ri=1716403","nofollow":false},{"id":6200,"name":"Magnetic Resonance Imaging","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Imaging?f_ri=1716403","nofollow":false},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=1716403","nofollow":false},{"id":60971,"name":"Cerebrospinal Fluid","url":"https://www.academia.edu/Documents/in/Cerebrospinal_Fluid?f_ri=1716403","nofollow":false},{"id":61474,"name":"Brain","url":"https://www.academia.edu/Documents/in/Brain?f_ri=1716403"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=1716403"},{"id":65615,"name":"Cerebellum","url":"https://www.academia.edu/Documents/in/Cerebellum?f_ri=1716403"},{"id":137516,"name":"Follow-up studies","url":"https://www.academia.edu/Documents/in/Follow-up_studies?f_ri=1716403"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403"},{"id":315168,"name":"Optic Neuritis","url":"https://www.academia.edu/Documents/in/Optic_Neuritis?f_ri=1716403"},{"id":710565,"name":"Annals","url":"https://www.academia.edu/Documents/in/Annals?f_ri=1716403"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2426000,"name":"Brain stem","url":"https://www.academia.edu/Documents/in/Brain_stem?f_ri=1716403"},{"id":2439414,"name":"Magnetic resonance image","url":"https://www.academia.edu/Documents/in/Magnetic_resonance_image?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20249037" data-work_id="20249037" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20249037/A_human_autoantibody_to_renal_collecting_duct_cells_associated_with_thyroid_and_gastric_autoimmunity_and_possibly_renal_tubular_acidosis">A human autoantibody to renal collecting duct cells associated with thyroid and gastric autoimmunity and possibly renal tubular acidosis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A complement fixing autoantibody reacting with certain renal collecting duct cells, possibly so called dark cells, is described in 113 patients. Clinically, the antibody was strongly associated with thyroid disorders and pernicious... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20249037" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A complement fixing autoantibody reacting with certain renal collecting duct cells, possibly so called dark cells, is described in 113 patients. Clinically, the antibody was strongly associated with thyroid disorders and pernicious anaemia. The patients also showed a markedly increased frequency of thyroid antibodies and antibodies to gastric parietal cells and to intrinsic factor. One of the patients had a distal renal tubular acidosis (RTA) and pernicious anaemia. The antibody was also found in all of three other patients with either latent or manifest RTA. RTA is associated with various immunological diseases, and the renal collecting duct cell antibody may turn out to be a marker of this disorder, either involved in its pathogenesis or representing a secondary phenomenon.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20249037" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c4cda0fdf75694d5aa2a4e18c69cfd5e" rel="nofollow" data-download="{"attachment_id":41106430,"asset_id":20249037,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41106430/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34941942" href="https://universitetetioslo.academia.edu/HansErikHeier">Hans Erik Heier</a><script data-card-contents-for-user="34941942" type="text/json">{"id":34941942,"first_name":"Hans Erik","last_name":"Heier","domain_name":"universitetetioslo","page_name":"HansErikHeier","display_name":"Hans Erik Heier","profile_url":"https://universitetetioslo.academia.edu/HansErikHeier?f_ri=1716403","photo":"https://0.academia-photos.com/34941942/18730464/18691448/s65_hans_erik.heier.jpg"}</script></span></span></li><li class="js-paper-rank-work_20249037 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20249037"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20249037, container: ".js-paper-rank-work_20249037", }); 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$(".js-view-count[data-work-id=20249037]").text(description); $(".js-view-count-work_20249037").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20249037").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20249037"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="76078" href="https://www.academia.edu/Documents/in/Thyroid_gland">Thyroid gland</a>, <script data-card-contents-for-ri="76078" type="text/json">{"id":76078,"name":"Thyroid gland","url":"https://www.academia.edu/Documents/in/Thyroid_gland?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="323597" href="https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique">Fluorescent Antibody Technique</a>, <script data-card-contents-for-ri="323597" type="text/json">{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="532052" href="https://www.academia.edu/Documents/in/Thyroid_Diseases">Thyroid Diseases</a><script data-card-contents-for-ri="532052" type="text/json">{"id":532052,"name":"Thyroid Diseases","url":"https://www.academia.edu/Documents/in/Thyroid_Diseases?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20249037]'), work: {"id":20249037,"title":"A human autoantibody to renal collecting duct cells associated with thyroid and gastric autoimmunity and possibly renal tubular acidosis","created_at":"2016-01-14T01:10:15.319-08:00","url":"https://www.academia.edu/20249037/A_human_autoantibody_to_renal_collecting_duct_cells_associated_with_thyroid_and_gastric_autoimmunity_and_possibly_renal_tubular_acidosis?f_ri=1716403","dom_id":"work_20249037","summary":"A complement fixing autoantibody reacting with certain renal collecting duct cells, possibly so called dark cells, is described in 113 patients. Clinically, the antibody was strongly associated with thyroid disorders and pernicious anaemia. The patients also showed a markedly increased frequency of thyroid antibodies and antibodies to gastric parietal cells and to intrinsic factor. One of the patients had a distal renal tubular acidosis (RTA) and pernicious anaemia. The antibody was also found in all of three other patients with either latent or manifest RTA. RTA is associated with various immunological diseases, and the renal collecting duct cell antibody may turn out to be a marker of this disorder, either involved in its pathogenesis or representing a secondary phenomenon.","downloadable_attachments":[{"id":41106430,"asset_id":20249037,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34941942,"first_name":"Hans Erik","last_name":"Heier","domain_name":"universitetetioslo","page_name":"HansErikHeier","display_name":"Hans Erik Heier","profile_url":"https://universitetetioslo.academia.edu/HansErikHeier?f_ri=1716403","photo":"https://0.academia-photos.com/34941942/18730464/18691448/s65_hans_erik.heier.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":76078,"name":"Thyroid gland","url":"https://www.academia.edu/Documents/in/Thyroid_gland?f_ri=1716403","nofollow":false},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique?f_ri=1716403","nofollow":false},{"id":532052,"name":"Thyroid Diseases","url":"https://www.academia.edu/Documents/in/Thyroid_Diseases?f_ri=1716403","nofollow":false},{"id":1030661,"name":"Stomach","url":"https://www.academia.edu/Documents/in/Stomach?f_ri=1716403"},{"id":1482586,"name":"Autoantibodies","url":"https://www.academia.edu/Documents/in/Autoantibodies?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17525641" data-work_id="17525641" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17525641/Chronic_human_parvovirus_B19_infection_associated_with_interstitial_lung_disease">Chronic human parvovirus B19 infection associated with interstitial lung disease</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17525641" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="71bf72d8cdada862f0ab5aba19822680" rel="nofollow" data-download="{"attachment_id":39558924,"asset_id":17525641,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button 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class="u-positionAbsolute" data-has-card-for-ri-list="17525641"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="37826" href="https://www.academia.edu/Documents/in/Biopsy">Biopsy</a>, <script data-card-contents-for-ri="37826" type="text/json">{"id":37826,"name":"Biopsy","url":"https://www.academia.edu/Documents/in/Biopsy?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64933" href="https://www.academia.edu/Documents/in/Child">Child</a>, <script data-card-contents-for-ri="64933" type="text/json">{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="97269" href="https://www.academia.edu/Documents/in/Chronic_Disease">Chronic Disease</a>, <script data-card-contents-for-ri="97269" type="text/json">{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="118339" href="https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction">Polymerase Chain Reaction</a><script data-card-contents-for-ri="118339" type="text/json">{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17525641]'), work: {"id":17525641,"title":"Chronic human parvovirus B19 infection associated with interstitial lung 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Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease?f_ri=1716403","nofollow":false},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1716403","nofollow":false},{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=1716403"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=1716403"},{"id":458182,"name":"Consanguinity","url":"https://www.academia.edu/Documents/in/Consanguinity?f_ri=1716403"},{"id":546419,"name":"Age Factors","url":"https://www.academia.edu/Documents/in/Age_Factors?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70464653" data-work_id="70464653" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/70464653/ADAMTS13_autoantibodies_in_patients_with_thrombotic_microangiopathies_and_other_immunomediated_diseases">ADAMTS13 autoantibodies in patients with thrombotic microangiopathies and other immunomediated diseases</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Autoantibodies neutralizing human ADAMTS13 (a disintegrin-like and metalloproteinase with thrombospondin type 1 motif), the metalloprotease that physiologically cleaves von Willebrand factor, are a major cause of severe deficiency of the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_70464653" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Autoantibodies neutralizing human ADAMTS13 (a disintegrin-like and metalloproteinase with thrombospondin type 1 motif), the metalloprotease that physiologically cleaves von Willebrand factor, are a major cause of severe deficiency of the protease and of acquired thrombotic ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70464653" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ff5648c013ae9403209d2c5905d441da" rel="nofollow" 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$(".js-view-count-work_70464653").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="70464653"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="66614" href="https://www.academia.edu/Documents/in/Systemic_Lupus_Erythematosus">Systemic Lupus Erythematosus</a>, <script data-card-contents-for-ri="66614" type="text/json">{"id":66614,"name":"Systemic Lupus Erythematosus","url":"https://www.academia.edu/Documents/in/Systemic_Lupus_Erythematosus?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71304" href="https://www.academia.edu/Documents/in/Autoimmune_diseases">Autoimmune diseases</a>, <script data-card-contents-for-ri="71304" type="text/json">{"id":71304,"name":"Autoimmune diseases","url":"https://www.academia.edu/Documents/in/Autoimmune_diseases?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="80043" href="https://www.academia.edu/Documents/in/von_Willebrand_factor">von Willebrand factor</a><script data-card-contents-for-ri="80043" type="text/json">{"id":80043,"name":"von Willebrand factor","url":"https://www.academia.edu/Documents/in/von_Willebrand_factor?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=70464653]'), work: {"id":70464653,"title":"ADAMTS13 autoantibodies in patients with thrombotic microangiopathies and other immunomediated diseases","created_at":"2022-02-04T15:46:25.533-08:00","url":"https://www.academia.edu/70464653/ADAMTS13_autoantibodies_in_patients_with_thrombotic_microangiopathies_and_other_immunomediated_diseases?f_ri=1716403","dom_id":"work_70464653","summary":"Autoantibodies neutralizing human ADAMTS13 (a disintegrin-like and metalloproteinase with thrombospondin type 1 motif), the metalloprotease that physiologically cleaves von Willebrand factor, are a major cause of severe deficiency of the protease and of acquired thrombotic ...","downloadable_attachments":[{"id":80207108,"asset_id":70464653,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44932601,"first_name":"Bernhard","last_name":"Lämmle","domain_name":"independent","page_name":"BernhardLämmle","display_name":"Bernhard Lämmle","profile_url":"https://independent.academia.edu/BernhardL%C3%A4mmle?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false},{"id":66614,"name":"Systemic Lupus Erythematosus","url":"https://www.academia.edu/Documents/in/Systemic_Lupus_Erythematosus?f_ri=1716403","nofollow":false},{"id":71304,"name":"Autoimmune diseases","url":"https://www.academia.edu/Documents/in/Autoimmune_diseases?f_ri=1716403","nofollow":false},{"id":80043,"name":"von Willebrand factor","url":"https://www.academia.edu/Documents/in/von_Willebrand_factor?f_ri=1716403","nofollow":false},{"id":100732,"name":"Blood","url":"https://www.academia.edu/Documents/in/Blood?f_ri=1716403"},{"id":138603,"name":"Thrombocytopenia","url":"https://www.academia.edu/Documents/in/Thrombocytopenia?f_ri=1716403"},{"id":227282,"name":"Primary Antiphospholipid Syndrome","url":"https://www.academia.edu/Documents/in/Primary_Antiphospholipid_Syndrome?f_ri=1716403"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403"},{"id":289330,"name":"Prevalence","url":"https://www.academia.edu/Documents/in/Prevalence?f_ri=1716403"},{"id":917176,"name":"Thrombotic Microangiopathy","url":"https://www.academia.edu/Documents/in/Thrombotic_Microangiopathy?f_ri=1716403"},{"id":1028649,"name":"Thrombotic Thrombocytopenic Purpura","url":"https://www.academia.edu/Documents/in/Thrombotic_Thrombocytopenic_Purpura?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1482586,"name":"Autoantibodies","url":"https://www.academia.edu/Documents/in/Autoantibodies?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1819399,"name":"Case Control Studies","url":"https://www.academia.edu/Documents/in/Case_Control_Studies?f_ri=1716403"},{"id":2538635,"name":"Antiphospholipid Syndrome","url":"https://www.academia.edu/Documents/in/Antiphospholipid_Syndrome?f_ri=1716403"},{"id":2892963,"name":"immunoglobulin M","url":"https://www.academia.edu/Documents/in/immunoglobulin_M?f_ri=1716403"},{"id":3789879,"name":"Cardiovascular medicine and haematology","url":"https://www.academia.edu/Documents/in/Cardiovascular_medicine_and_haematology?f_ri=1716403"},{"id":3789883,"name":"Paediatrics and reproductive medicine","url":"https://www.academia.edu/Documents/in/Paediatrics_and_reproductive_medicine?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70175241" data-work_id="70175241" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/70175241/Targeting_pro_inflammatory_cytokines_following_joint_injury_acute_intra_articular_inhibition_of_interleukin_1_following_knee_injury_prevents_post_traumatic_arthritis">Targeting pro-inflammatory cytokines following joint injury: acute intra-articular inhibition of interleukin-1 following knee injury prevents post-traumatic arthritis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Introduction: Post-traumatic arthritis (PTA) is a progressive, degenerative response to joint injury, such as articular fracture. The pro-inflammatory cytokines, interleukin 1(IL-1) and tumor necrosis factor alpha (TNF-α), are acutely... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_70175241" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Introduction: Post-traumatic arthritis (PTA) is a progressive, degenerative response to joint injury, such as articular fracture. The pro-inflammatory cytokines, interleukin 1(IL-1) and tumor necrosis factor alpha (TNF-α), are acutely elevated following joint injury and remain elevated for prolonged periods post-injury. To investigate the role of local and systemic inflammation in the development of post-traumatic arthritis, we targeted both the initial acute local inflammatory response and a prolonged 4 week systemic inflammatory response by inhibiting IL-1 or TNF-α following articular fracture in the mouse knee. Methods: Anti-cytokine agents, IL-1 receptor antagonist (IL-1Ra) or soluble TNF receptor II (sTNFRII), were administered either locally via an acute intra-articular injection or systemically for a prolonged 4 week period following articular fracture of the knee in C57BL/6 mice. The severity of arthritis was then assessed at 8 weeks post-injury in joint tissues via histology and micro computed tomography, and systemic and local biomarkers were assessed in serum and synovial fluid. Results: Intra-articular inhibition of IL-1 significantly reduced cartilage degeneration, synovial inflammation, and did not alter bone morphology following articular fracture. However, systemic inhibition of IL-1, and local or systemic inhibition of TNF provided no benefit or conversely led to increased arthritic changes in the joint tissues. Conclusion: These results show that intra-articular IL-1, rather than TNF-α, plays a critical role in the acute inflammatory phase of joint injury and can be inhibited locally to reduce post-traumatic arthritis following a closed articular fracture. Targeted local inhibition of IL-1 following joint injury may represent a novel treatment option for PTA.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70175241" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="59fbe192da4a9f24b8b6dd7cb47bb0f1" rel="nofollow" data-download="{"attachment_id":80023918,"asset_id":70175241,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/80023918/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44224693" href="https://independent.academia.edu/StevenOlson4">Steven Olson</a><script data-card-contents-for-user="44224693" type="text/json">{"id":44224693,"first_name":"Steven","last_name":"Olson","domain_name":"independent","page_name":"StevenOlson4","display_name":"Steven Olson","profile_url":"https://independent.academia.edu/StevenOlson4?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_70175241 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="70175241"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 70175241, container: ".js-paper-rank-work_70175241", }); });</script></li><li class="js-percentile-work_70175241 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 70175241; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_70175241"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_70175241 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="70175241"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 70175241; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=70175241]").text(description); $(".js-view-count-work_70175241").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_70175241").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="70175241"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12426" href="https://www.academia.edu/Documents/in/Treatment_Outcome">Treatment Outcome</a>, <script data-card-contents-for-ri="12426" type="text/json">{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a><script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=70175241]'), work: {"id":70175241,"title":"Targeting pro-inflammatory cytokines following joint injury: acute intra-articular inhibition of interleukin-1 following knee injury prevents post-traumatic arthritis","created_at":"2022-01-31T11:46:08.716-08:00","url":"https://www.academia.edu/70175241/Targeting_pro_inflammatory_cytokines_following_joint_injury_acute_intra_articular_inhibition_of_interleukin_1_following_knee_injury_prevents_post_traumatic_arthritis?f_ri=1716403","dom_id":"work_70175241","summary":"Introduction: Post-traumatic arthritis (PTA) is a progressive, degenerative response to joint injury, such as articular fracture. The pro-inflammatory cytokines, interleukin 1(IL-1) and tumor necrosis factor alpha (TNF-α), are acutely elevated following joint injury and remain elevated for prolonged periods post-injury. To investigate the role of local and systemic inflammation in the development of post-traumatic arthritis, we targeted both the initial acute local inflammatory response and a prolonged 4 week systemic inflammatory response by inhibiting IL-1 or TNF-α following articular fracture in the mouse knee. Methods: Anti-cytokine agents, IL-1 receptor antagonist (IL-1Ra) or soluble TNF receptor II (sTNFRII), were administered either locally via an acute intra-articular injection or systemically for a prolonged 4 week period following articular fracture of the knee in C57BL/6 mice. The severity of arthritis was then assessed at 8 weeks post-injury in joint tissues via histology and micro computed tomography, and systemic and local biomarkers were assessed in serum and synovial fluid. Results: Intra-articular inhibition of IL-1 significantly reduced cartilage degeneration, synovial inflammation, and did not alter bone morphology following articular fracture. However, systemic inhibition of IL-1, and local or systemic inhibition of TNF provided no benefit or conversely led to increased arthritic changes in the joint tissues. Conclusion: These results show that intra-articular IL-1, rather than TNF-α, plays a critical role in the acute inflammatory phase of joint injury and can be inhibited locally to reduce post-traumatic arthritis following a closed articular fracture. Targeted local inhibition of IL-1 following joint injury may represent a novel treatment option for PTA.","downloadable_attachments":[{"id":80023918,"asset_id":70175241,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44224693,"first_name":"Steven","last_name":"Olson","domain_name":"independent","page_name":"StevenOlson4","display_name":"Steven Olson","profile_url":"https://independent.academia.edu/StevenOlson4?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1716403","nofollow":false},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1716403","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=1716403"},{"id":423007,"name":"Knee injuries","url":"https://www.academia.edu/Documents/in/Knee_injuries?f_ri=1716403"},{"id":474029,"name":"Tumor necrosis factor-alpha","url":"https://www.academia.edu/Documents/in/Tumor_necrosis_factor-alpha?f_ri=1716403"},{"id":964185,"name":"Synovitis","url":"https://www.academia.edu/Documents/in/Synovitis?f_ri=1716403"},{"id":994186,"name":"X Ray Microtomography","url":"https://www.academia.edu/Documents/in/X_Ray_Microtomography?f_ri=1716403"},{"id":1145265,"name":"Knee Joint","url":"https://www.academia.edu/Documents/in/Knee_Joint?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin?f_ri=1716403"},{"id":2477047,"name":"articular fracture","url":"https://www.academia.edu/Documents/in/articular_fracture?f_ri=1716403"},{"id":4073093,"name":"Inflammation Mediators","url":"https://www.academia.edu/Documents/in/Inflammation_Mediators?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70003648" data-work_id="70003648" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/70003648/Site_directed_in_vitro_immunization_leads_to_a_complete_human_monoclonal_IgG4%CE%BB_that_binds_specifically_to_the_CDR2_region_of_CTLA_4_CD152_without_interfering_the_engagement_of_natural_ligands">Site-directed in vitro immunization leads to a complete human monoclonal IgG4λ that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: The ability to acquire fully human monoclonal antibodies (mAbs) with pre-defined specificities is critical to the development of molecular tags for the analysis of receptor function in addition to promising immunotherapeutics.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_70003648" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: The ability to acquire fully human monoclonal antibodies (mAbs) with pre-defined specificities is critical to the development of molecular tags for the analysis of receptor function in addition to promising immunotherapeutics. Yet most of the arriving affinity maturated and complete human immunoglobulin G (IgG) molecules, which are actually derived from single human B cells, have not widely been used to study the conserved self antigens (Ags) such as CD152 (cytotoxic T lymphocyte antigen-4, CTLA-4) because proper hosts are lacking. Results: Here we developed an optimized protocol for site-directed in vitro immunizing peripheral blood mononuclear cells (PBMC) by using a selected epitope of human CD152, an essential receptor involved in down-regulation of T cell activation. The resultant stable trioma cell lines constantly produce anti-CD152 mAb (γ4λhuCD152), which contains variable (V) regions of the heavy chain and the light chain derived from the VH3 and Vλ human germline genes, respectively, and yet displays an unusual IgG4 isotype. Interestingly, γ4λhuCD152 has a basic pI not commonly found in myeloid monoclonal IgG4λs as revealed by the isoelectric focusing (IEF) analysis. Furthermore, γ4λhuCD152 binds specifically, with nanomolar affinity, to an extracellular constituency encompassing the putative second complementarity determining region (CDR2) of CD152, whereby it can react to activated CD3 + cells. Conclusion: In a context of specific cell depletion and conditioned medium,in vitro induction of human Abs against a conserved self Ag was successfully acquired and a relatively basic mAb, γ4λhuCD152, with high affinity to CDR2 of CD152 was thus obtained. Application of such a human IgG4λ mAb with designated CDR2 specificity may impact upon and prefer for CD152 labeling both in situ and ex situ, as it does not affect the binding of endogenous B7 ligands and can localize into the confined immunological synapse which may otherwise prevent the access of whole IgG1 molecules.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70003648" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f0bddf71e7728ce4bcdddfafea2c90a7" rel="nofollow" data-download="{"attachment_id":79894638,"asset_id":70003648,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/79894638/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="67581861" href="https://independent.academia.edu/%E7%AB%8B%E5%BE%B7%E9%87%91">立德金</a><script data-card-contents-for-user="67581861" type="text/json">{"id":67581861,"first_name":"立德","last_name":"金","domain_name":"independent","page_name":"立德金","display_name":"立德金","profile_url":"https://independent.academia.edu/%E7%AB%8B%E5%BE%B7%E9%87%91?f_ri=1716403","photo":"https://0.academia-photos.com/67581861/26883802/25347002/s65__._.jpg"}</script></span></span></li><li class="js-paper-rank-work_70003648 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="70003648"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 70003648, container: ".js-paper-rank-work_70003648", }); 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$(".js-view-count[data-work-id=70003648]").text(description); $(".js-view-count-work_70003648").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_70003648").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="70003648"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="6599" href="https://www.academia.edu/Documents/in/Flow_Cytometry">Flow Cytometry</a>, <script data-card-contents-for-ri="6599" type="text/json">{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=70003648]'), work: {"id":70003648,"title":"Site-directed in vitro immunization leads to a complete human monoclonal IgG4λ that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands","created_at":"2022-01-29T23:33:41.895-08:00","url":"https://www.academia.edu/70003648/Site_directed_in_vitro_immunization_leads_to_a_complete_human_monoclonal_IgG4%CE%BB_that_binds_specifically_to_the_CDR2_region_of_CTLA_4_CD152_without_interfering_the_engagement_of_natural_ligands?f_ri=1716403","dom_id":"work_70003648","summary":"Background: The ability to acquire fully human monoclonal antibodies (mAbs) with pre-defined specificities is critical to the development of molecular tags for the analysis of receptor function in addition to promising immunotherapeutics. Yet most of the arriving affinity maturated and complete human immunoglobulin G (IgG) molecules, which are actually derived from single human B cells, have not widely been used to study the conserved self antigens (Ags) such as CD152 (cytotoxic T lymphocyte antigen-4, CTLA-4) because proper hosts are lacking. Results: Here we developed an optimized protocol for site-directed in vitro immunizing peripheral blood mononuclear cells (PBMC) by using a selected epitope of human CD152, an essential receptor involved in down-regulation of T cell activation. The resultant stable trioma cell lines constantly produce anti-CD152 mAb (γ4λhuCD152), which contains variable (V) regions of the heavy chain and the light chain derived from the VH3 and Vλ human germline genes, respectively, and yet displays an unusual IgG4 isotype. Interestingly, γ4λhuCD152 has a basic pI not commonly found in myeloid monoclonal IgG4λs as revealed by the isoelectric focusing (IEF) analysis. Furthermore, γ4λhuCD152 binds specifically, with nanomolar affinity, to an extracellular constituency encompassing the putative second complementarity determining region (CDR2) of CD152, whereby it can react to activated CD3 + cells. Conclusion: In a context of specific cell depletion and conditioned medium,in vitro induction of human Abs against a conserved self Ag was successfully acquired and a relatively basic mAb, γ4λhuCD152, with high affinity to CDR2 of CD152 was thus obtained. Application of such a human IgG4λ mAb with designated CDR2 specificity may impact upon and prefer for CD152 labeling both in situ and ex situ, as it does not affect the binding of endogenous B7 ligands and can localize into the confined immunological synapse which may otherwise prevent the access of whole IgG1 molecules.","downloadable_attachments":[{"id":79894638,"asset_id":70003648,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":67581861,"first_name":"立德","last_name":"金","domain_name":"independent","page_name":"立德金","display_name":"立德金","profile_url":"https://independent.academia.edu/%E7%AB%8B%E5%BE%B7%E9%87%91?f_ri=1716403","photo":"https://0.academia-photos.com/67581861/26883802/25347002/s65__._.jpg"}],"research_interests":[{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=1716403","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1716403","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=1716403"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody?f_ri=1716403"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=1716403"},{"id":1159038,"name":"Epitopes","url":"https://www.academia.edu/Documents/in/Epitopes?f_ri=1716403"},{"id":1222191,"name":"Ligands","url":"https://www.academia.edu/Documents/in/Ligands?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1312081,"name":"Light chain","url":"https://www.academia.edu/Documents/in/Light_chain?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1865795,"name":"Hybridomas","url":"https://www.academia.edu/Documents/in/Hybridomas?f_ri=1716403"},{"id":2054788,"name":"Isoelectric point","url":"https://www.academia.edu/Documents/in/Isoelectric_point?f_ri=1716403"},{"id":2058817,"name":"Immunoblotting","url":"https://www.academia.edu/Documents/in/Immunoblotting?f_ri=1716403"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=1716403"},{"id":2971099,"name":"Heavy Chain","url":"https://www.academia.edu/Documents/in/Heavy_Chain?f_ri=1716403"},{"id":3729223,"name":"Conditioned medium","url":"https://www.academia.edu/Documents/in/Conditioned_medium?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_66472620 coauthored" data-work_id="66472620" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66472620/Development_of_a_fluorescent_label_tool_based_on_lanthanide_nanophosphors_for_viral_biomedical_application">Development of a fluorescent label tool based on lanthanide nanophosphors for viral biomedical application</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We report for the first time the preparation of luminescent lanthanide nanomaterial (LLN) linked bioconjugates and their application as a label tool for recognizing virus in the processing line of vaccine industrial fabrication. Several... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_66472620" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We report for the first time the preparation of luminescent lanthanide nanomaterial (LLN) linked bioconjugates and their application as a label tool for recognizing virus in the processing line of vaccine industrial fabrication. Several LLNs with the nanostructure forms of particles or rods/wires with europium (III) and terbium (III) ions in lattices of vanadate, phosphate and metal organic complex were prepared to develop novel fluorescent conjugates able to be applied as labels in fluorescence immunoassay analysis of virus/vaccine. With regard to the LLNs, we have successfully synthesized nanoparticles around 10 nm of YVO 4 : Eu(III), with high emission in the red spectral region, nanorod and nanowire of TbPO 4 • H 2 O and Eu 1−x Tb x PO 4 • H 2 O, width 5-7 nm and length 300 nm, showing very bright luminescence in green, and core/shell nanosized Eu(III) and Tb(III)/Eu(III) complexes with naphthoyl trifluoroacetone and tri-n-octylphosphineoxide (Eu.NTA.TOPO@PVP, Eu X Tb 1−X .NTA.TOPO). The appropriated core/shell structures can play a double role, one for enhancing luminescence efficiency and another for providing nanophosphors with better stability in water media for facilitating the penetration of nanophosphor core into a biomedical environment. The organic functionalizations of the obtained LLNs were done through their surface encapsulation with a functional polysiloxane including active groups such as amine (NH 2), thiocyanate (SCN) or mecarpto (SH). The properties of functional sol-gel matrix have great influence on the luminescence properties, especially luminescence intensity of YVO 4 : Eu(III), Eu.NTA.TOPO@PVP, TbPO 4 • H 2 O and Eu x Tb 1−x PO 4 • H 2 O. Bioconjugation processes of the functionalized LLNs have been studied with some bioactive molecules such as biotin, protein immunoglobulin G (IgG) or bovine serum albumin (BSA). The results of LLN-bioconjugate linking with IgG for recognizing virus (vaccine) will be presented in brief. It is consistent to state that the LLN bioconjugates prepared from YVO 4 : Eu(III)-nanoparticles, TbPO 4 • H 2 O nanorod or wire and EuNTA.TOPO@PVP nanosized core/shell complex could be used as labels for recognizing virus in diagnosis or in vaccine production by use of the fluorescence immunoassay (FIA) method. The fluorescence images of the incubated specimens consisting of LLN bioconjugate and vaccine fabricate could be obtained well in terms of sharpness, reproductivity and stability.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66472620" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4881a98cfcc05eb2e3c8f994ddfb4eb0" rel="nofollow" data-download="{"attachment_id":77650424,"asset_id":66472620,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77650424/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="101570307" href="https://independent.academia.edu/LeQuocMinh">Le Quoc Minh</a><script data-card-contents-for-user="101570307" type="text/json">{"id":101570307,"first_name":"Le Quoc","last_name":"Minh","domain_name":"independent","page_name":"LeQuocMinh","display_name":"Le Quoc Minh","profile_url":"https://independent.academia.edu/LeQuocMinh?f_ri=1716403","photo":"https://0.academia-photos.com/101570307/22527471/21722529/s65_le_quoc.minh.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-66472620">+1</span><div class="hidden js-additional-users-66472620"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/KimTran195">Kim Tran</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-66472620'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-66472620').html(); 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Several LLNs with the nanostructure forms of particles or rods/wires with europium (III) and terbium (III) ions in lattices of vanadate, phosphate and metal organic complex were prepared to develop novel fluorescent conjugates able to be applied as labels in fluorescence immunoassay analysis of virus/vaccine. With regard to the LLNs, we have successfully synthesized nanoparticles around 10 nm of YVO 4 : Eu(III), with high emission in the red spectral region, nanorod and nanowire of TbPO 4 • H 2 O and Eu 1−x Tb x PO 4 • H 2 O, width 5-7 nm and length 300 nm, showing very bright luminescence in green, and core/shell nanosized Eu(III) and Tb(III)/Eu(III) complexes with naphthoyl trifluoroacetone and tri-n-octylphosphineoxide (Eu.NTA.TOPO@PVP, Eu X Tb 1−X .NTA.TOPO). The appropriated core/shell structures can play a double role, one for enhancing luminescence efficiency and another for providing nanophosphors with better stability in water media for facilitating the penetration of nanophosphor core into a biomedical environment. The organic functionalizations of the obtained LLNs were done through their surface encapsulation with a functional polysiloxane including active groups such as amine (NH 2), thiocyanate (SCN) or mecarpto (SH). The properties of functional sol-gel matrix have great influence on the luminescence properties, especially luminescence intensity of YVO 4 : Eu(III), Eu.NTA.TOPO@PVP, TbPO 4 • H 2 O and Eu x Tb 1−x PO 4 • H 2 O. Bioconjugation processes of the functionalized LLNs have been studied with some bioactive molecules such as biotin, protein immunoglobulin G (IgG) or bovine serum albumin (BSA). The results of LLN-bioconjugate linking with IgG for recognizing virus (vaccine) will be presented in brief. It is consistent to state that the LLN bioconjugates prepared from YVO 4 : Eu(III)-nanoparticles, TbPO 4 • H 2 O nanorod or wire and EuNTA.TOPO@PVP nanosized core/shell complex could be used as labels for recognizing virus in diagnosis or in vaccine production by use of the fluorescence immunoassay (FIA) method. The fluorescence images of the incubated specimens consisting of LLN bioconjugate and vaccine fabricate could be obtained well in terms of sharpness, reproductivity and stability.","downloadable_attachments":[{"id":77650424,"asset_id":66472620,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":101570307,"first_name":"Le Quoc","last_name":"Minh","domain_name":"independent","page_name":"LeQuocMinh","display_name":"Le Quoc Minh","profile_url":"https://independent.academia.edu/LeQuocMinh?f_ri=1716403","photo":"https://0.academia-photos.com/101570307/22527471/21722529/s65_le_quoc.minh.jpg"},{"id":212766885,"first_name":"Kim","last_name":"Tran","domain_name":"independent","page_name":"KimTran195","display_name":"Kim Tran","profile_url":"https://independent.academia.edu/KimTran195?f_ri=1716403","photo":"https://0.academia-photos.com/212766885/108885155/98120054/s65_kim.tran.png"}],"research_interests":[{"id":511,"name":"Materials Science","url":"https://www.academia.edu/Documents/in/Materials_Science?f_ri=1716403","nofollow":false},{"id":50628,"name":"Nanowires","url":"https://www.academia.edu/Documents/in/Nanowires?f_ri=1716403","nofollow":false},{"id":162813,"name":"Fluorescence Imaging","url":"https://www.academia.edu/Documents/in/Fluorescence_Imaging?f_ri=1716403","nofollow":false},{"id":422724,"name":"Shell Structure","url":"https://www.academia.edu/Documents/in/Shell_Structure?f_ri=1716403","nofollow":false},{"id":881608,"name":"Bovine Serum Albumin","url":"https://www.academia.edu/Documents/in/Bovine_Serum_Albumin?f_ri=1716403"},{"id":1267724,"name":"Biomedical Application","url":"https://www.academia.edu/Documents/in/Biomedical_Application?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_63944081" data-work_id="63944081" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/63944081/Serosurveillance_of_varicella_and_hepatitis_B_infection_after_reported_cases_in_medical_students_and_the_relationship_between_past_varicella_disease_history_and_immunity_status">Serosurveillance of varicella and hepatitis B infection after reported cases in medical students and the relationship between past varicella disease history and immunity status</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Objective: To identify seroprevalence of varicella and the relationship with their histories of experiences of varicella diseases and to provide appropriate immunization against varicella, mumps, measles, rubella and hepatitis B to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_63944081" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Objective: To identify seroprevalence of varicella and the relationship with their histories of experiences of varicella diseases and to provide appropriate immunization against varicella, mumps, measles, rubella and hepatitis B to medical students. Material and method: All of the medical students were eligible for participation after informed consents. Immunization history against varicella, mumps, measles, rubella (MMR) and hepatitis B were obtained from a questionnaire. A blood sample was obtained from each student for IgG antibody against VZV by ELISA. Medical students with an uncertain history or no documentation of hepatitis B vaccination were tested for HBsAg and anti-HBcIgG by ELISA. Results: There were 383 medical students enrolled. The mean age at enrollment was 21.6 years (median 21.4 years; range 18-25.8 years). Of 383 medical students, 372 (97.2%) had documents of receiving MMR immunizations. The blood samples were obtained from 374 of 383 (97.6%) medical students to id...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/63944081" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f7e56a08f3906f4dd1959a4a8dd27824" rel="nofollow" data-download="{"attachment_id":76200471,"asset_id":63944081,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/76200471/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="76538070" href="https://independent.academia.edu/PhutsapongSrisawat">Phutsapong Srisawat</a><script data-card-contents-for-user="76538070" type="text/json">{"id":76538070,"first_name":"Phutsapong","last_name":"Srisawat","domain_name":"independent","page_name":"PhutsapongSrisawat","display_name":"Phutsapong Srisawat","profile_url":"https://independent.academia.edu/PhutsapongSrisawat?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_63944081 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="63944081"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 63944081, container: ".js-paper-rank-work_63944081", }); 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$(".js-view-count[data-work-id=63944081]").text(description); $(".js-view-count-work_63944081").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_63944081").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="63944081"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="10096" href="https://www.academia.edu/Documents/in/Thailand">Thailand</a>, <script data-card-contents-for-ri="10096" type="text/json">{"id":10096,"name":"Thailand","url":"https://www.academia.edu/Documents/in/Thailand?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62445" href="https://www.academia.edu/Documents/in/Medical_Students">Medical Students</a>, <script data-card-contents-for-ri="62445" type="text/json">{"id":62445,"name":"Medical Students","url":"https://www.academia.edu/Documents/in/Medical_Students?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="63331" href="https://www.academia.edu/Documents/in/Hepatitis_B">Hepatitis B</a><script data-card-contents-for-ri="63331" type="text/json">{"id":63331,"name":"Hepatitis B","url":"https://www.academia.edu/Documents/in/Hepatitis_B?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=63944081]'), work: {"id":63944081,"title":"Serosurveillance of varicella and hepatitis B infection after reported cases in medical students and the relationship between past varicella disease history and immunity status","created_at":"2021-12-12T23:23:30.858-08:00","url":"https://www.academia.edu/63944081/Serosurveillance_of_varicella_and_hepatitis_B_infection_after_reported_cases_in_medical_students_and_the_relationship_between_past_varicella_disease_history_and_immunity_status?f_ri=1716403","dom_id":"work_63944081","summary":"Objective: To identify seroprevalence of varicella and the relationship with their histories of experiences of varicella diseases and to provide appropriate immunization against varicella, mumps, measles, rubella and hepatitis B to medical students. Material and method: All of the medical students were eligible for participation after informed consents. Immunization history against varicella, mumps, measles, rubella (MMR) and hepatitis B were obtained from a questionnaire. A blood sample was obtained from each student for IgG antibody against VZV by ELISA. Medical students with an uncertain history or no documentation of hepatitis B vaccination were tested for HBsAg and anti-HBcIgG by ELISA. Results: There were 383 medical students enrolled. The mean age at enrollment was 21.6 years (median 21.4 years; range 18-25.8 years). Of 383 medical students, 372 (97.2%) had documents of receiving MMR immunizations. The blood samples were obtained from 374 of 383 (97.6%) medical students to id...","downloadable_attachments":[{"id":76200471,"asset_id":63944081,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":76538070,"first_name":"Phutsapong","last_name":"Srisawat","domain_name":"independent","page_name":"PhutsapongSrisawat","display_name":"Phutsapong Srisawat","profile_url":"https://independent.academia.edu/PhutsapongSrisawat?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":10096,"name":"Thailand","url":"https://www.academia.edu/Documents/in/Thailand?f_ri=1716403","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false},{"id":62445,"name":"Medical Students","url":"https://www.academia.edu/Documents/in/Medical_Students?f_ri=1716403","nofollow":false},{"id":63331,"name":"Hepatitis B","url":"https://www.academia.edu/Documents/in/Hepatitis_B?f_ri=1716403","nofollow":false},{"id":78113,"name":"Mumps","url":"https://www.academia.edu/Documents/in/Mumps?f_ri=1716403"},{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=1716403"},{"id":146049,"name":"Rubella Virus","url":"https://www.academia.edu/Documents/in/Rubella_Virus?f_ri=1716403"},{"id":327850,"name":"Questionnaires","url":"https://www.academia.edu/Documents/in/Questionnaires?f_ri=1716403"},{"id":584601,"name":"Chi Square Distribution","url":"https://www.academia.edu/Documents/in/Chi_Square_Distribution?f_ri=1716403"},{"id":762837,"name":"Chickenpox","url":"https://www.academia.edu/Documents/in/Chickenpox?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1318932,"name":"Predictive value of tests","url":"https://www.academia.edu/Documents/in/Predictive_value_of_tests?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1769512,"name":"Measles","url":"https://www.academia.edu/Documents/in/Measles?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_63071748" data-work_id="63071748" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/63071748/Increased_retinal_endothelial_cell_monolayer_permeability_induced_by_the_diabetic_milieu_role_of_advanced_non_enzymatic_glycation_and_polyol_pathway_activation">Increased retinal endothelial cell monolayer permeability induced by the diabetic milieu: role of advanced non-enzymatic glycation and polyol pathway activation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/63071748" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b8b94fe81b347a00d295e9931b22f265" rel="nofollow" data-download="{"attachment_id":75619855,"asset_id":63071748,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75619855/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="31900796" href="https://independent.academia.edu/FPricci">F. Pricci</a><script data-card-contents-for-user="31900796" type="text/json">{"id":31900796,"first_name":"F.","last_name":"Pricci","domain_name":"independent","page_name":"FPricci","display_name":"F. Pricci","profile_url":"https://independent.academia.edu/FPricci?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_63071748 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="63071748"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 63071748, container: ".js-paper-rank-work_63071748", }); });</script></li><li class="js-percentile-work_63071748 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 63071748; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_63071748"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_63071748 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="63071748"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 63071748; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=63071748]").text(description); $(".js-view-count-work_63071748").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_63071748").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="63071748"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2184" href="https://www.academia.edu/Documents/in/Electron_Microscopy">Electron Microscopy</a>, <script data-card-contents-for-ri="2184" type="text/json">{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4581" href="https://www.academia.edu/Documents/in/Diabetes">Diabetes</a>, <script data-card-contents-for-ri="4581" type="text/json">{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12981" href="https://www.academia.edu/Documents/in/Enzyme_Inhibitors">Enzyme Inhibitors</a>, <script data-card-contents-for-ri="12981" type="text/json">{"id":12981,"name":"Enzyme Inhibitors","url":"https://www.academia.edu/Documents/in/Enzyme_Inhibitors?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="21466" href="https://www.academia.edu/Documents/in/Polymers">Polymers</a><script data-card-contents-for-ri="21466" type="text/json">{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=63071748]'), work: {"id":63071748,"title":"Increased retinal endothelial cell monolayer permeability induced by the diabetic milieu: role of advanced non-enzymatic glycation and polyol pathway activation","created_at":"2021-12-03T00:47:17.692-08:00","url":"https://www.academia.edu/63071748/Increased_retinal_endothelial_cell_monolayer_permeability_induced_by_the_diabetic_milieu_role_of_advanced_non_enzymatic_glycation_and_polyol_pathway_activation?f_ri=1716403","dom_id":"work_63071748","summary":null,"downloadable_attachments":[{"id":75619855,"asset_id":63071748,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":31900796,"first_name":"F.","last_name":"Pricci","domain_name":"independent","page_name":"FPricci","display_name":"F. Pricci","profile_url":"https://independent.academia.edu/FPricci?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=1716403","nofollow":false},{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes?f_ri=1716403","nofollow":false},{"id":12981,"name":"Enzyme Inhibitors","url":"https://www.academia.edu/Documents/in/Enzyme_Inhibitors?f_ri=1716403","nofollow":false},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers?f_ri=1716403","nofollow":false},{"id":96573,"name":"Diabetic Retinopathy","url":"https://www.academia.edu/Documents/in/Diabetic_Retinopathy?f_ri=1716403"},{"id":117200,"name":"Retina","url":"https://www.academia.edu/Documents/in/Retina?f_ri=1716403"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=1716403"},{"id":382388,"name":"Nitric Oxide Synthase","url":"https://www.academia.edu/Documents/in/Nitric_Oxide_Synthase?f_ri=1716403"},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell?f_ri=1716403"},{"id":710903,"name":"Sorbitol","url":"https://www.academia.edu/Documents/in/Sorbitol?f_ri=1716403"},{"id":788667,"name":"ENDOTHELIUM","url":"https://www.academia.edu/Documents/in/ENDOTHELIUM?f_ri=1716403"},{"id":801416,"name":"Guanidines","url":"https://www.academia.edu/Documents/in/Guanidines?f_ri=1716403"},{"id":881608,"name":"Bovine Serum Albumin","url":"https://www.academia.edu/Documents/in/Bovine_Serum_Albumin?f_ri=1716403"},{"id":1703031,"name":"Mannitol","url":"https://www.academia.edu/Documents/in/Mannitol?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2628237,"name":"Cell membrane permeability","url":"https://www.academia.edu/Documents/in/Cell_membrane_permeability?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_57495880" data-work_id="57495880" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/57495880/Vasculitis_Associated_With_Tumor_Necrosis_Factor_%CE%B1_Inhibitors">Vasculitis Associated With Tumor Necrosis Factor-α Inhibitors</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Objective: To describe the clinical characteristics, histopathologic features, and outcomes of patients in whom vasculitis developed in association with use of tumor necrosis factor-␣ (TNF-␣) inhibitors. Patients and Methods: This is a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_57495880" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Objective: To describe the clinical characteristics, histopathologic features, and outcomes of patients in whom vasculitis developed in association with use of tumor necrosis factor-␣ (TNF-␣) inhibitors. Patients and Methods: This is a retrospective review of patients evaluated at Mayo Clinic, Rochester, Minnesota, from January 1, 1998, through March 31, 2011, with a diagnosis of vasculitis induced by anti-TNF-␣ therapy. Results: Of 8 patients with vasculitis associated with anti-TNF-␣ therapy (mean age, 48.5 years), 6 (75%) were female. Four (50%) had rheumatoid arthritis, 1 (13%) had Crohn disease, and 3 (38%) had ulcerative colitis. Five (63%) were treated with infliximab, 2 (25%) with etanercept, and 1 (13%) with adalimumab. The mean duration of treatment before development of vasculitis was 34.5 months. The skin was the predominant organ affected (5 patients [63%]), with the most common cutaneous lesion being palpable purpura (4 of 5 [80%]). Two organs involved in systemic vasculitis were the peripheral nervous system (4 patients [50%]) and kidney (1 patient [13%]). All cases of vasculitis were histopathologically confirmed. Seven of 8 patients improved with discontinuation of therapy (mean time to resolution, 6.9 months) and adjuvant treatment (all 8 received prednisone; another agent was also used in 7); rechallenge with anti-TNF-␣ therapy was not attempted in any patient. At last follow-up, no patients had experienced a recurrence of vasculitis after therapy discontinuation. Conclusion: Cutaneous small-vessel vasculitis was the most common finding, but systemic vasculitis, including peripheral nerve and renal vasculitis, was also frequently observed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/57495880" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a5e371670e96f4286b2916a42e1cc949" rel="nofollow" data-download="{"attachment_id":72371940,"asset_id":57495880,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72371940/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38646581" href="https://independent.academia.edu/WetterDavid">David Wetter</a><script data-card-contents-for-user="38646581" type="text/json">{"id":38646581,"first_name":"David","last_name":"Wetter","domain_name":"independent","page_name":"WetterDavid","display_name":"David Wetter","profile_url":"https://independent.academia.edu/WetterDavid?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_57495880 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="57495880"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 57495880, container: ".js-paper-rank-work_57495880", }); });</script></li><li class="js-percentile-work_57495880 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 57495880; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_57495880"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_57495880 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="57495880"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 57495880; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=57495880]").text(description); $(".js-view-count-work_57495880").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_57495880").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="57495880"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="12733" href="https://www.academia.edu/Documents/in/Rheumatoid_Arthritis">Rheumatoid Arthritis</a>, <script data-card-contents-for-ri="12733" type="text/json">{"id":12733,"name":"Rheumatoid Arthritis","url":"https://www.academia.edu/Documents/in/Rheumatoid_Arthritis?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="140057" href="https://www.academia.edu/Documents/in/Hematuria">Hematuria</a>, <script data-card-contents-for-ri="140057" type="text/json">{"id":140057,"name":"Hematuria","url":"https://www.academia.edu/Documents/in/Hematuria?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="147196" href="https://www.academia.edu/Documents/in/Monoclonal_Antibodies">Monoclonal Antibodies</a>, <script data-card-contents-for-ri="147196" type="text/json">{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="289271" href="https://www.academia.edu/Documents/in/Aged">Aged</a><script data-card-contents-for-ri="289271" type="text/json">{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=57495880]'), work: {"id":57495880,"title":"Vasculitis Associated With Tumor Necrosis Factor-α Inhibitors","created_at":"2021-10-13T05:07:32.523-07:00","url":"https://www.academia.edu/57495880/Vasculitis_Associated_With_Tumor_Necrosis_Factor_%CE%B1_Inhibitors?f_ri=1716403","dom_id":"work_57495880","summary":"Objective: To describe the clinical characteristics, histopathologic features, and outcomes of patients in whom vasculitis developed in association with use of tumor necrosis factor-␣ (TNF-␣) inhibitors. Patients and Methods: This is a retrospective review of patients evaluated at Mayo Clinic, Rochester, Minnesota, from January 1, 1998, through March 31, 2011, with a diagnosis of vasculitis induced by anti-TNF-␣ therapy. Results: Of 8 patients with vasculitis associated with anti-TNF-␣ therapy (mean age, 48.5 years), 6 (75%) were female. Four (50%) had rheumatoid arthritis, 1 (13%) had Crohn disease, and 3 (38%) had ulcerative colitis. Five (63%) were treated with infliximab, 2 (25%) with etanercept, and 1 (13%) with adalimumab. The mean duration of treatment before development of vasculitis was 34.5 months. The skin was the predominant organ affected (5 patients [63%]), with the most common cutaneous lesion being palpable purpura (4 of 5 [80%]). Two organs involved in systemic vasculitis were the peripheral nervous system (4 patients [50%]) and kidney (1 patient [13%]). All cases of vasculitis were histopathologically confirmed. Seven of 8 patients improved with discontinuation of therapy (mean time to resolution, 6.9 months) and adjuvant treatment (all 8 received prednisone; another agent was also used in 7); rechallenge with anti-TNF-␣ therapy was not attempted in any patient. At last follow-up, no patients had experienced a recurrence of vasculitis after therapy discontinuation. Conclusion: Cutaneous small-vessel vasculitis was the most common finding, but systemic vasculitis, including peripheral nerve and renal vasculitis, was also frequently observed.","downloadable_attachments":[{"id":72371940,"asset_id":57495880,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38646581,"first_name":"David","last_name":"Wetter","domain_name":"independent","page_name":"WetterDavid","display_name":"David Wetter","profile_url":"https://independent.academia.edu/WetterDavid?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":12733,"name":"Rheumatoid Arthritis","url":"https://www.academia.edu/Documents/in/Rheumatoid_Arthritis?f_ri=1716403","nofollow":false},{"id":140057,"name":"Hematuria","url":"https://www.academia.edu/Documents/in/Hematuria?f_ri=1716403","nofollow":false},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403","nofollow":false},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403","nofollow":false},{"id":438985,"name":"Methotrexate","url":"https://www.academia.edu/Documents/in/Methotrexate?f_ri=1716403"},{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=1716403"},{"id":777616,"name":"Proteinuria","url":"https://www.academia.edu/Documents/in/Proteinuria?f_ri=1716403"},{"id":1198588,"name":"Cyclophosphamide","url":"https://www.academia.edu/Documents/in/Cyclophosphamide?f_ri=1716403"},{"id":1205432,"name":"Mayo Clinic Proceedings","url":"https://www.academia.edu/Documents/in/Mayo_Clinic_Proceedings?f_ri=1716403"},{"id":1293308,"name":"Glucocorticoids","url":"https://www.academia.edu/Documents/in/Glucocorticoids?f_ri=1716403"},{"id":1351088,"name":"Crohn Disease","url":"https://www.academia.edu/Documents/in/Crohn_Disease?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1740531,"name":"Azathioprine","url":"https://www.academia.edu/Documents/in/Azathioprine?f_ri=1716403"},{"id":3483787,"name":"Prednisone","url":"https://www.academia.edu/Documents/in/Prednisone?f_ri=1716403"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_55699174" data-work_id="55699174" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/55699174/Fast_immobilization_of_probe_beads_by_dielectrophoresis_controlled_adhesion_in_a_versatile_microfluidic_platform_for_affinity_assay">Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay The use of probe beads for lab-on-chip affinity assays is very interesting from a practical point of view.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_55699174" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay The use of probe beads for lab-on-chip affinity assays is very interesting from a practical point of view. It is easier to handle and trap beads than molecules in microfluidic systems. We present a method for the immobilization of probe beads at defined areas on a chip using dielectrophoresis (DEP)-controlled adhesion. The method is fast, i.e., it takes between 10 and 120 s-depending on the protocol-to functionalize a chip surface at defined areas. The method is versatile, i.e., it works for beads with different types of probe molecule coatings. The immobilization is irreversible, i.e., the retained beads are able to withstand high flow velocities in a flow-through device even after the DEP voltage is turned off, thus allowing the use of conventional high-conductivity analyte buffers in the following assay procedure. We demonstrate the on-chip immobilization of fluorescent beads coated with biotin, protein A, and goat-antimouse immunoglobulin G (IgG). The number of immobilized beads at an electrode array can be determined from their fluorescence signal. Further, we use this method to demonstrate the detection of streptavidin and mouse IgG. Finally, we demonstrate the feasibility of the parallel detection of different analyte molecules on the same chip.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/55699174" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2fb0a20474f1bda397fb2f5796890469" rel="nofollow" data-download="{"attachment_id":71447706,"asset_id":55699174,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/71447706/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="122244394" href="https://independent.academia.edu/ThomasStaubli">Thomas Staubli</a><script data-card-contents-for-user="122244394" type="text/json">{"id":122244394,"first_name":"Thomas","last_name":"Staubli","domain_name":"independent","page_name":"ThomasStaubli","display_name":"Thomas Staubli","profile_url":"https://independent.academia.edu/ThomasStaubli?f_ri=1716403","photo":"https://0.academia-photos.com/122244394/30557471/28305186/s65_thomas.staubli.jpg"}</script></span></span></li><li class="js-paper-rank-work_55699174 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="55699174"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 55699174, container: ".js-paper-rank-work_55699174", }); 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$(".js-view-count[data-work-id=55699174]").text(description); $(".js-view-count-work_55699174").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_55699174").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="55699174"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="72" href="https://www.academia.edu/Documents/in/Chemical_Engineering">Chemical Engineering</a>, <script data-card-contents-for-ri="72" type="text/json">{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="524" href="https://www.academia.edu/Documents/in/Analytical_Chemistry">Analytical Chemistry</a>, <script data-card-contents-for-ri="524" type="text/json">{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2721" href="https://www.academia.edu/Documents/in/Microfluidics">Microfluidics</a>, <script data-card-contents-for-ri="2721" type="text/json">{"id":2721,"name":"Microfluidics","url":"https://www.academia.edu/Documents/in/Microfluidics?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="27372" href="https://www.academia.edu/Documents/in/Dielectrophoresis">Dielectrophoresis</a><script data-card-contents-for-ri="27372" type="text/json">{"id":27372,"name":"Dielectrophoresis","url":"https://www.academia.edu/Documents/in/Dielectrophoresis?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=55699174]'), work: {"id":55699174,"title":"Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay","created_at":"2021-10-05T12:03:14.457-07:00","url":"https://www.academia.edu/55699174/Fast_immobilization_of_probe_beads_by_dielectrophoresis_controlled_adhesion_in_a_versatile_microfluidic_platform_for_affinity_assay?f_ri=1716403","dom_id":"work_55699174","summary":"Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay The use of probe beads for lab-on-chip affinity assays is very interesting from a practical point of view. It is easier to handle and trap beads than molecules in microfluidic systems. We present a method for the immobilization of probe beads at defined areas on a chip using dielectrophoresis (DEP)-controlled adhesion. The method is fast, i.e., it takes between 10 and 120 s-depending on the protocol-to functionalize a chip surface at defined areas. The method is versatile, i.e., it works for beads with different types of probe molecule coatings. The immobilization is irreversible, i.e., the retained beads are able to withstand high flow velocities in a flow-through device even after the DEP voltage is turned off, thus allowing the use of conventional high-conductivity analyte buffers in the following assay procedure. We demonstrate the on-chip immobilization of fluorescent beads coated with biotin, protein A, and goat-antimouse immunoglobulin G (IgG). The number of immobilized beads at an electrode array can be determined from their fluorescence signal. Further, we use this method to demonstrate the detection of streptavidin and mouse IgG. Finally, we demonstrate the feasibility of the parallel detection of different analyte molecules on the same chip.","downloadable_attachments":[{"id":71447706,"asset_id":55699174,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":122244394,"first_name":"Thomas","last_name":"Staubli","domain_name":"independent","page_name":"ThomasStaubli","display_name":"Thomas Staubli","profile_url":"https://independent.academia.edu/ThomasStaubli?f_ri=1716403","photo":"https://0.academia-photos.com/122244394/30557471/28305186/s65_thomas.staubli.jpg"}],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=1716403","nofollow":false},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=1716403","nofollow":false},{"id":2721,"name":"Microfluidics","url":"https://www.academia.edu/Documents/in/Microfluidics?f_ri=1716403","nofollow":false},{"id":27372,"name":"Dielectrophoresis","url":"https://www.academia.edu/Documents/in/Dielectrophoresis?f_ri=1716403","nofollow":false},{"id":201209,"name":"Biotin","url":"https://www.academia.edu/Documents/in/Biotin?f_ri=1716403"},{"id":201213,"name":"Streptavidin","url":"https://www.academia.edu/Documents/in/Streptavidin?f_ri=1716403"},{"id":371425,"name":"Electrophoresis","url":"https://www.academia.edu/Documents/in/Electrophoresis?f_ri=1716403"},{"id":501187,"name":"Miniaturization","url":"https://www.academia.edu/Documents/in/Miniaturization?f_ri=1716403"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres?f_ri=1716403"},{"id":1275015,"name":"Bead","url":"https://www.academia.edu/Documents/in/Bead?f_ri=1716403"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2550937,"name":"Molecular probes","url":"https://www.academia.edu/Documents/in/Molecular_probes?f_ri=1716403"},{"id":2816680,"name":"Microfluidic Analytical Techniques","url":"https://www.academia.edu/Documents/in/Microfluidic_Analytical_Techniques?f_ri=1716403"},{"id":3211364,"name":"Staphylococcal protein A","url":"https://www.academia.edu/Documents/in/Staphylococcal_protein_A?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_53021774" data-work_id="53021774" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/53021774/Diphtheria_toxin_IgG_levels_in_military_and_civilian_blood_donors_in_Rio_de_Janeiro_Brazil">Diphtheria toxin IgG levels in military and civilian blood donors in Rio de Janeiro, Brazil</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Serologic data on diseases that are preventable by vaccines are necessary to evaluate the success of immunization programs and to identify susceptible subgroups. In the present study, we determined serum IgG levels against diphtheria... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_53021774" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Serologic data on diseases that are preventable by vaccines are necessary to evaluate the success of immunization programs and to identify susceptible subgroups. In the present study, we determined serum IgG levels against diphtheria toxin of military and civilian blood donors (N = 75; 69.3% males and 30.7% females) aged 18-64 years, from the Brazilian Army Biology Institute, Rio de Janeiro, using a commercial diphtheria kit (Diphtheria IgG ELISA; IBL, Germany). Most (63%) unprotected military donors were from the older age group of 41 to 64 years. In contrast, the majority (71%) of young military donors (18 to 30 years) were fully protected. About half of the military donors aged 31 to 40 years were protected against diphtheria. Among the civilians, about 50% of persons aged 18 to 30 years and 31 to 40 years had protective antibody levels against diphtheria as also did 64% of individuals aged 41 to 64 years. All civilians had a similar antibody response (geometric mean = 0.55 IU/mL) independent of age group. Military donors aged 18-30 years had higher IgG levels (geometric mean = 0.82 IU/mL) than military donors of 41-64 years (geometric mean = 0.51 IU/mL; P > 0.05). In conclusion, the existence of a considerable proportion of susceptible adults supports the position that reliable data on the immune status of the population should be maintained routinely and emphasizes the importance of adequate immunization during adulthood.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/53021774" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="eadee3c2ad67f51aeafbf9e1f04d865f" rel="nofollow" data-download="{"attachment_id":70001599,"asset_id":53021774,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/70001599/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="161614727" href="https://uerj.academia.edu/LucimarMilagres">Lucimar Milagres</a><script data-card-contents-for-user="161614727" type="text/json">{"id":161614727,"first_name":"Lucimar","last_name":"Milagres","domain_name":"uerj","page_name":"LucimarMilagres","display_name":"Lucimar Milagres","profile_url":"https://uerj.academia.edu/LucimarMilagres?f_ri=1716403","photo":"https://0.academia-photos.com/161614727/79498885/68065096/s65_lucimar.milagres.jpeg"}</script></span></span></li><li class="js-paper-rank-work_53021774 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="53021774"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 53021774, container: ".js-paper-rank-work_53021774", }); });</script></li><li class="js-percentile-work_53021774 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53021774; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_53021774"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_53021774 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="53021774"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53021774; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53021774]").text(description); $(".js-view-count-work_53021774").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_53021774").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="53021774"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="9821" href="https://www.academia.edu/Documents/in/Brazil">Brazil</a>, <script data-card-contents-for-ri="9821" type="text/json">{"id":9821,"name":"Brazil","url":"https://www.academia.edu/Documents/in/Brazil?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a>, <script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="104217" href="https://www.academia.edu/Documents/in/Rio_de_Janeiro">Rio de Janeiro</a>, <script data-card-contents-for-ri="104217" type="text/json">{"id":104217,"name":"Rio de Janeiro","url":"https://www.academia.edu/Documents/in/Rio_de_Janeiro?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="133057" href="https://www.academia.edu/Documents/in/Young_Adult">Young Adult</a><script data-card-contents-for-ri="133057" type="text/json">{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=53021774]'), work: {"id":53021774,"title":"Diphtheria toxin IgG levels in military and civilian blood donors in Rio de Janeiro, Brazil","created_at":"2021-09-20T10:25:51.531-07:00","url":"https://www.academia.edu/53021774/Diphtheria_toxin_IgG_levels_in_military_and_civilian_blood_donors_in_Rio_de_Janeiro_Brazil?f_ri=1716403","dom_id":"work_53021774","summary":"Serologic data on diseases that are preventable by vaccines are necessary to evaluate the success of immunization programs and to identify susceptible subgroups. In the present study, we determined serum IgG levels against diphtheria toxin of military and civilian blood donors (N = 75; 69.3% males and 30.7% females) aged 18-64 years, from the Brazilian Army Biology Institute, Rio de Janeiro, using a commercial diphtheria kit (Diphtheria IgG ELISA; IBL, Germany). Most (63%) unprotected military donors were from the older age group of 41 to 64 years. In contrast, the majority (71%) of young military donors (18 to 30 years) were fully protected. About half of the military donors aged 31 to 40 years were protected against diphtheria. Among the civilians, about 50% of persons aged 18 to 30 years and 31 to 40 years had protective antibody levels against diphtheria as also did 64% of individuals aged 41 to 64 years. All civilians had a similar antibody response (geometric mean = 0.55 IU/mL) independent of age group. Military donors aged 18-30 years had higher IgG levels (geometric mean = 0.82 IU/mL) than military donors of 41-64 years (geometric mean = 0.51 IU/mL; P \u003e 0.05). In conclusion, the existence of a considerable proportion of susceptible adults supports the position that reliable data on the immune status of the population should be maintained routinely and emphasizes the importance of adequate immunization during adulthood.","downloadable_attachments":[{"id":70001599,"asset_id":53021774,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":161614727,"first_name":"Lucimar","last_name":"Milagres","domain_name":"uerj","page_name":"LucimarMilagres","display_name":"Lucimar Milagres","profile_url":"https://uerj.academia.edu/LucimarMilagres?f_ri=1716403","photo":"https://0.academia-photos.com/161614727/79498885/68065096/s65_lucimar.milagres.jpeg"}],"research_interests":[{"id":9821,"name":"Brazil","url":"https://www.academia.edu/Documents/in/Brazil?f_ri=1716403","nofollow":false},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=1716403","nofollow":false},{"id":104217,"name":"Rio de Janeiro","url":"https://www.academia.edu/Documents/in/Rio_de_Janeiro?f_ri=1716403","nofollow":false},{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=1716403","nofollow":false},{"id":483419,"name":"Brazilian","url":"https://www.academia.edu/Documents/in/Brazilian?f_ri=1716403"},{"id":679066,"name":"Blood Donors","url":"https://www.academia.edu/Documents/in/Blood_Donors?f_ri=1716403"},{"id":1268687,"name":"Military Personnel","url":"https://www.academia.edu/Documents/in/Military_Personnel?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1817270,"name":"Diphtheria","url":"https://www.academia.edu/Documents/in/Diphtheria?f_ri=1716403"},{"id":1944474,"name":"Diphtheria Toxin","url":"https://www.academia.edu/Documents/in/Diphtheria_Toxin?f_ri=1716403"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=1716403"},{"id":3947089,"name":"Blood Donor","url":"https://www.academia.edu/Documents/in/Blood_Donor?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_26078846" data-work_id="26078846" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/26078846/Oligoclonal_fingerprint_of_CSF_IgG_in_multiple_sclerosis_patients_is_not_modified_following_intrathecal_administration_of_natural_beta_interferon">Oligoclonal "fingerprint" of CSF IgG in multiple sclerosis patients is not modified following intrathecal administration of natural beta-interferon</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The IgG pattern in CSF was studied in 11 patients with multiple sclerosis who exhibited an oligoclonal banding upon thin-layer polyacrylamide gel isoelectric focusing followed by silver stain of unconcentrated CSF. Each patient received... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_26078846" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The IgG pattern in CSF was studied in 11 patients with multiple sclerosis who exhibited an oligoclonal banding upon thin-layer polyacrylamide gel isoelectric focusing followed by silver stain of unconcentrated CSF. Each patient received beta-interferon intrathecally during a 2 month period. No modification was observed over a 6 month period. In addition, the oligoclonal pattern was remarkably unique for each individual representing a typical "fingerprint" which allowed the identification of any single CSF.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/26078846" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1fa22b04109ce2c187164c764c89cfe9" rel="nofollow" data-download="{"attachment_id":46430191,"asset_id":26078846,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46430191/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="49977186" href="https://independent.academia.edu/ColetteChapuisCellier">Colette Chapuis-Cellier</a><script data-card-contents-for-user="49977186" type="text/json">{"id":49977186,"first_name":"Colette","last_name":"Chapuis-Cellier","domain_name":"independent","page_name":"ColetteChapuisCellier","display_name":"Colette Chapuis-Cellier","profile_url":"https://independent.academia.edu/ColetteChapuisCellier?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_26078846 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="26078846"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 26078846, container: ".js-paper-rank-work_26078846", }); 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Each patient received beta-interferon intrathecally during a 2 month period. No modification was observed over a 6 month period. In addition, the oligoclonal pattern was remarkably unique for each individual representing a typical \"fingerprint\" which allowed the identification of any single CSF.","downloadable_attachments":[{"id":46430191,"asset_id":26078846,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":49977186,"first_name":"Colette","last_name":"Chapuis-Cellier","domain_name":"independent","page_name":"ColetteChapuisCellier","display_name":"Colette Chapuis-Cellier","profile_url":"https://independent.academia.edu/ColetteChapuisCellier?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3097,"name":"Multiple sclerosis","url":"https://www.academia.edu/Documents/in/Multiple_sclerosis?f_ri=1716403","nofollow":false},{"id":193464,"name":"Sodium Dodecyl Sulfate-Polyacrylamide Gel 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href="https://www.academia.edu/24792670/Exploring_the_repertoire_of_IgE_binding_self_antigens_associated_with_atopic_eczema">Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24792670" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="73f39051c31353ff33b06d190ba104b8" rel="nofollow" data-download="{"attachment_id":45120996,"asset_id":24792670,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45120996/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35910352" href="https://independent.academia.edu/RetoCrameri">Reto Crameri</a><script data-card-contents-for-user="35910352" type="text/json">{"id":35910352,"first_name":"Reto","last_name":"Crameri","domain_name":"independent","page_name":"RetoCrameri","display_name":"Reto 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class="u-positionAbsolute" data-has-card-for-ri-list="24792670"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62896" href="https://www.academia.edu/Documents/in/Allergy">Allergy</a>, <script data-card-contents-for-ri="62896" type="text/json">{"id":62896,"name":"Allergy","url":"https://www.academia.edu/Documents/in/Allergy?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="176180" href="https://www.academia.edu/Documents/in/Immunoglobulin_E">Immunoglobulin E</a>, <script data-card-contents-for-ri="176180" type="text/json">{"id":176180,"name":"Immunoglobulin E","url":"https://www.academia.edu/Documents/in/Immunoglobulin_E?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="213343" href="https://www.academia.edu/Documents/in/Superoxide_Dismutase">Superoxide Dismutase</a><script data-card-contents-for-ri="213343" type="text/json">{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24792670]'), work: {"id":24792670,"title":"Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema","created_at":"2016-04-26T22:03:46.538-07:00","url":"https://www.academia.edu/24792670/Exploring_the_repertoire_of_IgE_binding_self_antigens_associated_with_atopic_eczema?f_ri=1716403","dom_id":"work_24792670","summary":null,"downloadable_attachments":[{"id":45120996,"asset_id":24792670,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35910352,"first_name":"Reto","last_name":"Crameri","domain_name":"independent","page_name":"RetoCrameri","display_name":"Reto Crameri","profile_url":"https://independent.academia.edu/RetoCrameri?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":62896,"name":"Allergy","url":"https://www.academia.edu/Documents/in/Allergy?f_ri=1716403","nofollow":false},{"id":176180,"name":"Immunoglobulin E","url":"https://www.academia.edu/Documents/in/Immunoglobulin_E?f_ri=1716403","nofollow":false},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=1716403","nofollow":false},{"id":279546,"name":"Association","url":"https://www.academia.edu/Documents/in/Association?f_ri=1716403"},{"id":374916,"name":"IGE","url":"https://www.academia.edu/Documents/in/IGE?f_ri=1716403"},{"id":591601,"name":"Atopic Dermatitis","url":"https://www.academia.edu/Documents/in/Atopic_Dermatitis?f_ri=1716403"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins?f_ri=1716403"},{"id":1385358,"name":"Translation initiation","url":"https://www.academia.edu/Documents/in/Translation_initiation?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1826493,"name":"Clinical Allergy and Immunology","url":"https://www.academia.edu/Documents/in/Clinical_Allergy_and_Immunology?f_ri=1716403"},{"id":1864827,"name":"Autoantigens","url":"https://www.academia.edu/Documents/in/Autoantigens?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23520894" data-work_id="23520894" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23520894/Amino_acid_differences_in_the_n_terminus_of_CH2_influence_the_relative_abilities_of_IgG2_AND_IgG3_to_activate_complement">Amino acid differences in the n-terminus of CH2 influence the relative abilities of IgG2 AND IgG3 to activate complement</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The four human IgG isotypes are highly conserved in amino acid sequence, but show differential ability to activate complement (C'): IgG3 and IgGl are very active. IgG2 is active under certain conditions, and IgG4 is inactive. Although the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23520894" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The four human IgG isotypes are highly conserved in amino acid sequence, but show differential ability to activate complement (C'): IgG3 and IgGl are very active. IgG2 is active under certain conditions, and IgG4 is inactive. Although the second constant domain (C,2) is critical for C' activation, the individual amino acids that confer isotype-specific activity have not been identified. We have generated a series of mutants between IgG2 and IgG3, resulting in the exchange of the four N-terminal and six C-terminal polymorphic residues within C"2. Mutants containing the N-terminus of the C,2 of IgG3 were as effective as wildtype IgG3 in Clq binding, Cl activation and terminal complex (MAC) formation, but had reduced ability to effect C/-mediated lysis. IgG2 and mutants containing the N-terminal portion of the CH2 of IgG2 were reduced compared to IgG3 in activating Cl, binding Clq and inducing assembly of the MAC, and were inactive in mediating lysis of target cells. Thus, the amino acid sequence differences in the N-terminus of C,,2 play a critical role in determining the relative abilities of IgG2 and IgG3 to bind Clq and activate the C' cascade although additional residues of C,2 must be involved in mediating optimal target cells lysis. The sequence of the N-terminus of C,2 was less critical in determining C4 and C3 binding. Characterization of domain exchange mutants suggests that intermediate steps may be partly dependent on domains other than C,2. IgGs that do not direct target cell lysis nevertheless activate intermediate steps in the pathway, which may contribute to immune complex-associated disorders. pi;, 1997 Elsevier Science Ltd. All rights reserved.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23520894" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1d55221a4f5df0d4ecd49637978936a4" rel="nofollow" data-download="{"attachment_id":43956290,"asset_id":23520894,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43956290/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="7101847" href="https://independent.academia.edu/SherieLMorrison">Sherie L. Morrison</a><script data-card-contents-for-user="7101847" type="text/json">{"id":7101847,"first_name":"Sherie L.","last_name":"Morrison","domain_name":"independent","page_name":"SherieLMorrison","display_name":"Sherie L. Morrison","profile_url":"https://independent.academia.edu/SherieLMorrison?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23520894 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23520894"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23520894, container: ".js-paper-rank-work_23520894", }); });</script></li><li class="js-percentile-work_23520894 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23520894; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_23520894"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_23520894 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="23520894"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23520894; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23520894]").text(description); $(".js-view-count-work_23520894").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_23520894").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="23520894"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2705" href="https://www.academia.edu/Documents/in/Complement_activation">Complement activation</a>, <script data-card-contents-for-ri="2705" type="text/json">{"id":2705,"name":"Complement activation","url":"https://www.academia.edu/Documents/in/Complement_activation?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57896" href="https://www.academia.edu/Documents/in/DNS">DNS</a>, <script data-card-contents-for-ri="57896" type="text/json">{"id":57896,"name":"DNS","url":"https://www.academia.edu/Documents/in/DNS?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="63332" href="https://www.academia.edu/Documents/in/Molecular_Immunology">Molecular Immunology</a><script data-card-contents-for-ri="63332" type="text/json">{"id":63332,"name":"Molecular Immunology","url":"https://www.academia.edu/Documents/in/Molecular_Immunology?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23520894]'), work: {"id":23520894,"title":"Amino acid differences in the n-terminus of CH2 influence the relative abilities of IgG2 AND IgG3 to activate complement","created_at":"2016-03-21T09:47:01.582-07:00","url":"https://www.academia.edu/23520894/Amino_acid_differences_in_the_n_terminus_of_CH2_influence_the_relative_abilities_of_IgG2_AND_IgG3_to_activate_complement?f_ri=1716403","dom_id":"work_23520894","summary":"The four human IgG isotypes are highly conserved in amino acid sequence, but show differential ability to activate complement (C'): IgG3 and IgGl are very active. IgG2 is active under certain conditions, and IgG4 is inactive. Although the second constant domain (C,2) is critical for C' activation, the individual amino acids that confer isotype-specific activity have not been identified. We have generated a series of mutants between IgG2 and IgG3, resulting in the exchange of the four N-terminal and six C-terminal polymorphic residues within C\"2. Mutants containing the N-terminus of the C,2 of IgG3 were as effective as wildtype IgG3 in Clq binding, Cl activation and terminal complex (MAC) formation, but had reduced ability to effect C/-mediated lysis. IgG2 and mutants containing the N-terminal portion of the CH2 of IgG2 were reduced compared to IgG3 in activating Cl, binding Clq and inducing assembly of the MAC, and were inactive in mediating lysis of target cells. Thus, the amino acid sequence differences in the N-terminus of C,,2 play a critical role in determining the relative abilities of IgG2 and IgG3 to bind Clq and activate the C' cascade although additional residues of C,2 must be involved in mediating optimal target cells lysis. The sequence of the N-terminus of C,2 was less critical in determining C4 and C3 binding. Characterization of domain exchange mutants suggests that intermediate steps may be partly dependent on domains other than C,2. IgGs that do not direct target cell lysis nevertheless activate intermediate steps in the pathway, which may contribute to immune complex-associated disorders. pi;, 1997 Elsevier Science Ltd. All rights reserved.","downloadable_attachments":[{"id":43956290,"asset_id":23520894,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":7101847,"first_name":"Sherie L.","last_name":"Morrison","domain_name":"independent","page_name":"SherieLMorrison","display_name":"Sherie L. Morrison","profile_url":"https://independent.academia.edu/SherieLMorrison?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":2705,"name":"Complement activation","url":"https://www.academia.edu/Documents/in/Complement_activation?f_ri=1716403","nofollow":false},{"id":57896,"name":"DNS","url":"https://www.academia.edu/Documents/in/DNS?f_ri=1716403","nofollow":false},{"id":63332,"name":"Molecular Immunology","url":"https://www.academia.edu/Documents/in/Molecular_Immunology?f_ri=1716403","nofollow":false},{"id":67401,"name":"Mutagenesis","url":"https://www.academia.edu/Documents/in/Mutagenesis?f_ri=1716403"},{"id":80694,"name":"Silver","url":"https://www.academia.edu/Documents/in/Silver?f_ri=1716403"},{"id":233880,"name":"MAC","url":"https://www.academia.edu/Documents/in/MAC?f_ri=1716403"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile?f_ri=1716403"},{"id":588623,"name":"Complement","url":"https://www.academia.edu/Documents/in/Complement?f_ri=1716403"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=1716403"},{"id":1185612,"name":"H","url":"https://www.academia.edu/Documents/in/H?f_ri=1716403"},{"id":1188997,"name":"C","url":"https://www.academia.edu/Documents/in/C?f_ri=1716403"},{"id":1192453,"name":"L","url":"https://www.academia.edu/Documents/in/L?f_ri=1716403"},{"id":1426712,"name":"Immunoglobulin","url":"https://www.academia.edu/Documents/in/Immunoglobulin?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16313459 coauthored" data-work_id="16313459" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16313459/Immunoglobulin_heavy_light_chain_analysis_enhances_the_detection_of_residual_disease_and_monitoring_of_multiple_myeloma_patients">Immunoglobulin heavy/light chain analysis enhances the detection of residual disease and monitoring of multiple myeloma patients</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To evaluate the clinical utility of incorporating a novel heavy/light chain immunoassay (HLC) into the existing methods for the assessment of multiple myeloma (MM) patients. Convenience sera samples from 90 previously treated IgG and IgA... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16313459" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To evaluate the clinical utility of incorporating a novel heavy/light chain immunoassay (HLC) into the existing methods for the assessment of multiple myeloma (MM) patients. Convenience sera samples from 90 previously treated IgG and IgA MM patients in different disease stages were analyzed. The study was conducted in Clinical Hospital Center Zagreb between 2011 and 2013. The collected sera were analyzed by standard laboratory techniques (serum protein electrophoresis, quantification of total immunoglobulins, serum immunofixation, serum free light chain [FLC] assay) and HLC assay. HLC ratios outside the normal range were found in 58 of 90 patients, including 28 out of 61 patients with total immunoglobulin measurements within the normal range and 5 out of 23 patients in complete response. Both elevated HLC isotype level and abnormal HLC ratio correlated with the parameters of tumor burden, including percentage of plasma cells in the bone marrow (P&lt;0.001 and P=0.002, respectively) ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16313459" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c15219b5d815ba8c9a1758c2e34db425" rel="nofollow" data-download="{"attachment_id":42533716,"asset_id":16313459,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42533716/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35505618" href="https://independent.academia.edu/RadmanI">I. Radman</a><script data-card-contents-for-user="35505618" type="text/json">{"id":35505618,"first_name":"I.","last_name":"Radman","domain_name":"independent","page_name":"RadmanI","display_name":"I. Radman","profile_url":"https://independent.academia.edu/RadmanI?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-16313459">+1</span><div class="hidden js-additional-users-16313459"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/KlaraDubravcic">Klara Dubravcic</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-16313459'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-16313459').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_16313459 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16313459"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16313459, container: ".js-paper-rank-work_16313459", }); });</script></li><li class="js-percentile-work_16313459 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16313459; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16313459"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16313459 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16313459"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16313459; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16313459]").text(description); $(".js-view-count-work_16313459").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16313459").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16313459"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="111110" href="https://www.academia.edu/Documents/in/Multiple_Myeloma">Multiple Myeloma</a>, <script data-card-contents-for-ri="111110" type="text/json">{"id":111110,"name":"Multiple Myeloma","url":"https://www.academia.edu/Documents/in/Multiple_Myeloma?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="192721" href="https://www.academia.edu/Documents/in/Risk_factors">Risk factors</a>, <script data-card-contents-for-ri="192721" type="text/json">{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="289271" href="https://www.academia.edu/Documents/in/Aged">Aged</a>, <script data-card-contents-for-ri="289271" type="text/json">{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="489727" href="https://www.academia.edu/Documents/in/Prognosis">Prognosis</a><script data-card-contents-for-ri="489727" type="text/json">{"id":489727,"name":"Prognosis","url":"https://www.academia.edu/Documents/in/Prognosis?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16313459]'), work: {"id":16313459,"title":"Immunoglobulin heavy/light chain analysis enhances the detection of residual disease and monitoring of multiple myeloma patients","created_at":"2015-09-30T00:36:56.008-07:00","url":"https://www.academia.edu/16313459/Immunoglobulin_heavy_light_chain_analysis_enhances_the_detection_of_residual_disease_and_monitoring_of_multiple_myeloma_patients?f_ri=1716403","dom_id":"work_16313459","summary":"To evaluate the clinical utility of incorporating a novel heavy/light chain immunoassay (HLC) into the existing methods for the assessment of multiple myeloma (MM) patients. Convenience sera samples from 90 previously treated IgG and IgA MM patients in different disease stages were analyzed. The study was conducted in Clinical Hospital Center Zagreb between 2011 and 2013. The collected sera were analyzed by standard laboratory techniques (serum protein electrophoresis, quantification of total immunoglobulins, serum immunofixation, serum free light chain [FLC] assay) and HLC assay. HLC ratios outside the normal range were found in 58 of 90 patients, including 28 out of 61 patients with total immunoglobulin measurements within the normal range and 5 out of 23 patients in complete response. Both elevated HLC isotype level and abnormal HLC ratio correlated with the parameters of tumor burden, including percentage of plasma cells in the bone marrow (P\u0026lt;0.001 and P=0.002, respectively) ...","downloadable_attachments":[{"id":42533716,"asset_id":16313459,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35505618,"first_name":"I.","last_name":"Radman","domain_name":"independent","page_name":"RadmanI","display_name":"I. Radman","profile_url":"https://independent.academia.edu/RadmanI?f_ri=1716403","photo":"/images/s65_no_pic.png"},{"id":35421753,"first_name":"Klara","last_name":"Dubravcic","domain_name":"independent","page_name":"KlaraDubravcic","display_name":"Klara Dubravcic","profile_url":"https://independent.academia.edu/KlaraDubravcic?f_ri=1716403","photo":"https://0.academia-photos.com/35421753/31150486/28720242/s65_klara.dubravcic.jpg"}],"research_interests":[{"id":111110,"name":"Multiple Myeloma","url":"https://www.academia.edu/Documents/in/Multiple_Myeloma?f_ri=1716403","nofollow":false},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=1716403","nofollow":false},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403","nofollow":false},{"id":489727,"name":"Prognosis","url":"https://www.academia.edu/Documents/in/Prognosis?f_ri=1716403","nofollow":false},{"id":511535,"name":"Immunoassay","url":"https://www.academia.edu/Documents/in/Immunoassay?f_ri=1716403"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20336627" data-work_id="20336627" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20336627/Immune_response_induced_by_recombinant_BCG_expressing_merozoite_surface_antigen_2_from_Plasmodium_falciparum">Immune response induced by recombinant BCG expressing merozoite surface antigen 2 from Plasmodium falciparum</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Mycobacterium bovis bacillus Calmette-Guerin (BCG) has been used as a live bacterial vaccine to immunize >3 billion people against tuberculosis. In an attempt to use this vaccinal strain as a vehicle for protective antigens, the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20336627" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Mycobacterium bovis bacillus Calmette-Guerin (BCG) has been used as a live bacterial vaccine to immunize >3 billion people against tuberculosis. In an attempt to use this vaccinal strain as a vehicle for protective antigens, the recombinant BCG (rBCG), expressing merozoite surface antigen 2 (MSA2) from Plasmodium falciparum under the control of an expression cassette carrying the promoter of heat shock protein 70 (HSP70) from M. tuberculosis, was constructed and used to immunize BABL/c mice. The administration of rBCG producing MSA2 (BCG-MSA2) resulted in the induction of a strong humoral and cellular response directed against MSA2. These results encourage the further protection testing of BCG-MSA2 vaccines in primate models.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20336627" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a847b697a82125f5e06c90037de63300" rel="nofollow" data-download="{"attachment_id":41295374,"asset_id":20336627,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41295374/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41635340" href="https://independent.academia.edu/ChunfuZheng">Chunfu Zheng</a><script data-card-contents-for-user="41635340" type="text/json">{"id":41635340,"first_name":"Chunfu","last_name":"Zheng","domain_name":"independent","page_name":"ChunfuZheng","display_name":"Chunfu Zheng","profile_url":"https://independent.academia.edu/ChunfuZheng?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_20336627 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20336627"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20336627, container: ".js-paper-rank-work_20336627", }); });</script></li><li class="js-percentile-work_20336627 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20336627; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_20336627"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_20336627 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="20336627"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20336627; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20336627]").text(description); $(".js-view-count-work_20336627").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20336627").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20336627"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2702" href="https://www.academia.edu/Documents/in/Immune_response">Immune response</a>, <script data-card-contents-for-ri="2702" type="text/json">{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="150847" href="https://www.academia.edu/Documents/in/Vaccine">Vaccine</a><script data-card-contents-for-ri="150847" type="text/json">{"id":150847,"name":"Vaccine","url":"https://www.academia.edu/Documents/in/Vaccine?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20336627]'), work: {"id":20336627,"title":"Immune response induced by recombinant BCG expressing merozoite surface antigen 2 from Plasmodium falciparum","created_at":"2016-01-17T16:12:00.075-08:00","url":"https://www.academia.edu/20336627/Immune_response_induced_by_recombinant_BCG_expressing_merozoite_surface_antigen_2_from_Plasmodium_falciparum?f_ri=1716403","dom_id":"work_20336627","summary":"Mycobacterium bovis bacillus Calmette-Guerin (BCG) has been used as a live bacterial vaccine to immunize \u003e3 billion people against tuberculosis. In an attempt to use this vaccinal strain as a vehicle for protective antigens, the recombinant BCG (rBCG), expressing merozoite surface antigen 2 (MSA2) from Plasmodium falciparum under the control of an expression cassette carrying the promoter of heat shock protein 70 (HSP70) from M. tuberculosis, was constructed and used to immunize BABL/c mice. The administration of rBCG producing MSA2 (BCG-MSA2) resulted in the induction of a strong humoral and cellular response directed against MSA2. These results encourage the further protection testing of BCG-MSA2 vaccines in primate models.","downloadable_attachments":[{"id":41295374,"asset_id":20336627,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41635340,"first_name":"Chunfu","last_name":"Zheng","domain_name":"independent","page_name":"ChunfuZheng","display_name":"Chunfu Zheng","profile_url":"https://independent.academia.edu/ChunfuZheng?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=1716403","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false},{"id":150847,"name":"Vaccine","url":"https://www.academia.edu/Documents/in/Vaccine?f_ri=1716403","nofollow":false},{"id":454230,"name":"Plasmodium falciparum","url":"https://www.academia.edu/Documents/in/Plasmodium_falciparum?f_ri=1716403"},{"id":744838,"name":"Protozoan Proteins","url":"https://www.academia.edu/Documents/in/Protozoan_Proteins?f_ri=1716403"},{"id":765872,"name":"Heat Shock Protein","url":"https://www.academia.edu/Documents/in/Heat_Shock_Protein?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1895642,"name":"Malaria Vaccines","url":"https://www.academia.edu/Documents/in/Malaria_Vaccines?f_ri=1716403"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin?f_ri=1716403"},{"id":2058663,"name":"Interferon gamma","url":"https://www.academia.edu/Documents/in/Interferon_gamma?f_ri=1716403"},{"id":2212745,"name":"BCG vaccine","url":"https://www.academia.edu/Documents/in/BCG_vaccine?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20163174 coauthored" data-work_id="20163174" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20163174/Effects_of_the_administration_of_cholera_toxin_as_a_mucosal_adjuvant_on_the_immune_and_protective_response_induced_by_Proteus_mirabilis_MrpA_fimbrial_protein_in_the_urinary_tract">Effects of the administration of cholera toxin as a mucosal adjuvant on the immune and protective response induced by Proteus mirabilis MrpA fimbrial protein in the urinary tract</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Proteus mirabilis is commonly associated with complicated UTI and expresses several virulence factors, including MR/P fimbriae. In the present study mice were immunised nasally with MrpA, the structural subunit of MR/P, with or without CT... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20163174" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Proteus mirabilis is commonly associated with complicated UTI and expresses several virulence factors, including MR/P fimbriae. In the present study mice were immunised nasally with MrpA, the structural subunit of MR/P, with or without CT as a mucosal adjuvant. The animals were then challenged with P. mirabilis and induction of specific serum and urine IgG and IgA, IFN-γ production and bacterial kidney and bladder colonization were assessed. MrpA-immunised mice exhibited significant induction of serum IgA and urine IgA and IgG. MrpA/CT-immunised mice showed both significant serum and urine IgA and IgG production. Only this group showed significant IFN-γ production. Both groups of animals had significant decrease in bacterial colonization of kidneys but not of bladders. No correlation between specific antibody induction in serum and CFU decrease was observed in any group of animals. Our results suggest that a mucosal adjuvant (CT) in the urinary tract enhanced humoral and cytokine response although it did not influence the degree of protection against UTI provided by MrpA. Further studies are necessary to understand immune modulation in the urinary tract.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20163174" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6fa655230a72a16a149887aaf297cb9e" rel="nofollow" data-download="{"attachment_id":41983768,"asset_id":20163174,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41983768/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41278404" href="https://iibce.academia.edu/PaolaScavone">Paola Scavone</a><script data-card-contents-for-user="41278404" type="text/json">{"id":41278404,"first_name":"Paola","last_name":"Scavone","domain_name":"iibce","page_name":"PaolaScavone","display_name":"Paola Scavone","profile_url":"https://iibce.academia.edu/PaolaScavone?f_ri=1716403","photo":"https://0.academia-photos.com/41278404/11201538/12499054/s65_paola.scavone.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-20163174">+1</span><div class="hidden js-additional-users-20163174"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://udelar.academia.edu/Anal%C3%ADaRial">Analía Rial</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-20163174'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-20163174').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_20163174 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20163174"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20163174, container: ".js-paper-rank-work_20163174", }); });</script></li><li class="js-percentile-work_20163174 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20163174; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_20163174"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_20163174 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="20163174"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20163174; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20163174]").text(description); $(".js-view-count-work_20163174").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20163174").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20163174"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71294" href="https://www.academia.edu/Documents/in/Kidney">Kidney</a><script data-card-contents-for-ri="71294" type="text/json">{"id":71294,"name":"Kidney","url":"https://www.academia.edu/Documents/in/Kidney?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20163174]'), work: {"id":20163174,"title":"Effects of the administration of cholera toxin as a mucosal adjuvant on the immune and protective response induced by Proteus mirabilis MrpA fimbrial protein in the urinary tract","created_at":"2016-01-11T06:13:42.670-08:00","url":"https://www.academia.edu/20163174/Effects_of_the_administration_of_cholera_toxin_as_a_mucosal_adjuvant_on_the_immune_and_protective_response_induced_by_Proteus_mirabilis_MrpA_fimbrial_protein_in_the_urinary_tract?f_ri=1716403","dom_id":"work_20163174","summary":"Proteus mirabilis is commonly associated with complicated UTI and expresses several virulence factors, including MR/P fimbriae. In the present study mice were immunised nasally with MrpA, the structural subunit of MR/P, with or without CT as a mucosal adjuvant. The animals were then challenged with P. mirabilis and induction of specific serum and urine IgG and IgA, IFN-γ production and bacterial kidney and bladder colonization were assessed. MrpA-immunised mice exhibited significant induction of serum IgA and urine IgA and IgG. MrpA/CT-immunised mice showed both significant serum and urine IgA and IgG production. Only this group showed significant IFN-γ production. Both groups of animals had significant decrease in bacterial colonization of kidneys but not of bladders. No correlation between specific antibody induction in serum and CFU decrease was observed in any group of animals. Our results suggest that a mucosal adjuvant (CT) in the urinary tract enhanced humoral and cytokine response although it did not influence the degree of protection against UTI provided by MrpA. Further studies are necessary to understand immune modulation in the urinary tract.","downloadable_attachments":[{"id":41983768,"asset_id":20163174,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41278404,"first_name":"Paola","last_name":"Scavone","domain_name":"iibce","page_name":"PaolaScavone","display_name":"Paola Scavone","profile_url":"https://iibce.academia.edu/PaolaScavone?f_ri=1716403","photo":"https://0.academia-photos.com/41278404/11201538/12499054/s65_paola.scavone.jpg"},{"id":59024408,"first_name":"Analía","last_name":"Rial","domain_name":"udelar","page_name":"AnalíaRial","display_name":"Analía Rial","profile_url":"https://udelar.academia.edu/Anal%C3%ADaRial?f_ri=1716403","photo":"https://0.academia-photos.com/59024408/15972843/16466294/s65_anal_a.rial.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1716403","nofollow":false},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1716403","nofollow":false},{"id":71294,"name":"Kidney","url":"https://www.academia.edu/Documents/in/Kidney?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403"},{"id":158884,"name":"Urinary Tract Infections","url":"https://www.academia.edu/Documents/in/Urinary_Tract_Infections?f_ri=1716403"},{"id":184383,"name":"Microbiology and Immunology","url":"https://www.academia.edu/Documents/in/Microbiology_and_Immunology?f_ri=1716403"},{"id":363553,"name":"Urinary Tract","url":"https://www.academia.edu/Documents/in/Urinary_Tract?f_ri=1716403"},{"id":719914,"name":"Cholera toxin-b","url":"https://www.academia.edu/Documents/in/Cholera_toxin-b?f_ri=1716403"},{"id":1463687,"name":"Proteus Mirabilis","url":"https://www.academia.edu/Documents/in/Proteus_Mirabilis?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1902592,"name":"Urinary Bladder","url":"https://www.academia.edu/Documents/in/Urinary_Bladder?f_ri=1716403"},{"id":2058663,"name":"Interferon gamma","url":"https://www.academia.edu/Documents/in/Interferon_gamma?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19750256" data-work_id="19750256" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19750256/Safety_of_intra_articular_injection_of_etanercept_in_small_joint_arthritis_an_uncontrolled_pilot_study_with_independent_imaging_assessment">Safety of intra-articular injection of etanercept in small-joint arthritis: an uncontrolled, pilot-study with independent imaging assessment</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This study was conducted to test the safety of intra-articular tumor necrosis factor alpha (TNF-α) antagonists in small joints with arthritis. A dose of 2-8 mg etanercept was given intra-articularly guided by ultrasonography (US) in 26... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19750256" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This study was conducted to test the safety of intra-articular tumor necrosis factor alpha (TNF-α) antagonists in small joints with arthritis. A dose of 2-8 mg etanercept was given intra-articularly guided by ultrasonography (US) in 26 patients with a flare of arthritis in a particular joint (16 wrists, two elbows, two ankles, six finger joints). Primary end points were imaging analyses by independent investigators: US-Doppler measurements were performed in all patients before and after the injection and MRI before and after were obtained in nine patients. The only adverse event was a case of swelling of the hand lasting 2 days after a wrist injection. Two patients had a supplementary glucocorticoid injection and were excluded from efficacy analysis after 4 days and 3 weeks, respectively. VAS for pain decreased after 1 week in 23 of 25 patients (median 0.62), and after 1 month in 14 of 24 patients (median 0.60). No significant changes were seen in erythrocyte sedimentation rate (ESR) or C-reactive protein (CRP). On MRI, all nine cases tested had a reduction in synovial thickness (P = 0.008) and US Doppler signals diminished after treatment (resistance index (RI) P = 0.02, pixels P = 0.09). In conclusion, intra-articular injection of etanercept gave no noticeable adverse events.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19750256" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f27700a180c05e387419d42b6ff50ebe" rel="nofollow" data-download="{"attachment_id":40822468,"asset_id":19750256,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40822468/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="40417907" href="https://independent.academia.edu/LeneTerslev">Lene Terslev</a><script data-card-contents-for-user="40417907" type="text/json">{"id":40417907,"first_name":"Lene","last_name":"Terslev","domain_name":"independent","page_name":"LeneTerslev","display_name":"Lene Terslev","profile_url":"https://independent.academia.edu/LeneTerslev?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19750256 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19750256"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19750256, container: ".js-paper-rank-work_19750256", }); 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$(".js-view-count[data-work-id=19750256]").text(description); $(".js-view-count-work_19750256").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19750256").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19750256"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="6200" href="https://www.academia.edu/Documents/in/Magnetic_Resonance_Imaging">Magnetic Resonance Imaging</a>, <script data-card-contents-for-ri="6200" type="text/json">{"id":6200,"name":"Magnetic Resonance Imaging","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Imaging?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15639" href="https://www.academia.edu/Documents/in/Ultrasound">Ultrasound</a>, <script data-card-contents-for-ri="15639" type="text/json">{"id":15639,"name":"Ultrasound","url":"https://www.academia.edu/Documents/in/Ultrasound?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="90702" href="https://www.academia.edu/Documents/in/Ultrasonography">Ultrasonography</a>, <script data-card-contents-for-ri="90702" type="text/json">{"id":90702,"name":"Ultrasonography","url":"https://www.academia.edu/Documents/in/Ultrasonography?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="137516" href="https://www.academia.edu/Documents/in/Follow-up_studies">Follow-up studies</a><script data-card-contents-for-ri="137516" type="text/json">{"id":137516,"name":"Follow-up studies","url":"https://www.academia.edu/Documents/in/Follow-up_studies?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19750256]'), work: {"id":19750256,"title":"Safety of intra-articular injection of etanercept in small-joint arthritis: an uncontrolled, pilot-study with independent imaging assessment","created_at":"2015-12-20T00:55:15.769-08:00","url":"https://www.academia.edu/19750256/Safety_of_intra_articular_injection_of_etanercept_in_small_joint_arthritis_an_uncontrolled_pilot_study_with_independent_imaging_assessment?f_ri=1716403","dom_id":"work_19750256","summary":"This study was conducted to test the safety of intra-articular tumor necrosis factor alpha (TNF-α) antagonists in small joints with arthritis. A dose of 2-8 mg etanercept was given intra-articularly guided by ultrasonography (US) in 26 patients with a flare of arthritis in a particular joint (16 wrists, two elbows, two ankles, six finger joints). Primary end points were imaging analyses by independent investigators: US-Doppler measurements were performed in all patients before and after the injection and MRI before and after were obtained in nine patients. The only adverse event was a case of swelling of the hand lasting 2 days after a wrist injection. Two patients had a supplementary glucocorticoid injection and were excluded from efficacy analysis after 4 days and 3 weeks, respectively. VAS for pain decreased after 1 week in 23 of 25 patients (median 0.62), and after 1 month in 14 of 24 patients (median 0.60). No significant changes were seen in erythrocyte sedimentation rate (ESR) or C-reactive protein (CRP). On MRI, all nine cases tested had a reduction in synovial thickness (P = 0.008) and US Doppler signals diminished after treatment (resistance index (RI) P = 0.02, pixels P = 0.09). In conclusion, intra-articular injection of etanercept gave no noticeable adverse events.","downloadable_attachments":[{"id":40822468,"asset_id":19750256,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":40417907,"first_name":"Lene","last_name":"Terslev","domain_name":"independent","page_name":"LeneTerslev","display_name":"Lene Terslev","profile_url":"https://independent.academia.edu/LeneTerslev?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":6200,"name":"Magnetic Resonance Imaging","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Imaging?f_ri=1716403","nofollow":false},{"id":15639,"name":"Ultrasound","url":"https://www.academia.edu/Documents/in/Ultrasound?f_ri=1716403","nofollow":false},{"id":90702,"name":"Ultrasonography","url":"https://www.academia.edu/Documents/in/Ultrasonography?f_ri=1716403","nofollow":false},{"id":137516,"name":"Follow-up studies","url":"https://www.academia.edu/Documents/in/Follow-up_studies?f_ri=1716403","nofollow":false},{"id":155231,"name":"Wrist joint","url":"https://www.academia.edu/Documents/in/Wrist_joint?f_ri=1716403"},{"id":191096,"name":"Arthritis","url":"https://www.academia.edu/Documents/in/Arthritis?f_ri=1716403"},{"id":227334,"name":"Resistive index","url":"https://www.academia.edu/Documents/in/Resistive_index?f_ri=1716403"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403"},{"id":295452,"name":"C reactive protein","url":"https://www.academia.edu/Documents/in/C_reactive_protein?f_ri=1716403"},{"id":308908,"name":"Pilot study","url":"https://www.academia.edu/Documents/in/Pilot_study?f_ri=1716403"},{"id":352624,"name":"Elbow Joint","url":"https://www.academia.edu/Documents/in/Elbow_Joint?f_ri=1716403"},{"id":425873,"name":"Adverse Event","url":"https://www.academia.edu/Documents/in/Adverse_Event?f_ri=1716403"},{"id":474029,"name":"Tumor necrosis factor-alpha","url":"https://www.academia.edu/Documents/in/Tumor_necrosis_factor-alpha?f_ri=1716403"},{"id":740993,"name":"Ankle Joint","url":"https://www.academia.edu/Documents/in/Ankle_Joint?f_ri=1716403"},{"id":903017,"name":"Erythrocyte Sedimentation Rate","url":"https://www.academia.edu/Documents/in/Erythrocyte_Sedimentation_Rate?f_ri=1716403"},{"id":945595,"name":"Pilot Projects","url":"https://www.academia.edu/Documents/in/Pilot_Projects?f_ri=1716403"},{"id":1293308,"name":"Glucocorticoids","url":"https://www.academia.edu/Documents/in/Glucocorticoids?f_ri=1716403"},{"id":1418396,"name":"Finger Joint","url":"https://www.academia.edu/Documents/in/Finger_Joint?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_65910014" data-work_id="65910014" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/65910014/Combination_of_the_two_schistosomal_antigens_Sm14_and_Sm29_elicits_significant_protection_against_experimental_Schistosoma_mansoni_infection">Combination of the two schistosomal antigens Sm14 and Sm29 elicits significant protection against experimental Schistosoma mansoni infection</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">h i g h l i g h t s Combination of Sm14 and Sm29 antigens was tested in S. mansoni infection model. Significant reduction of S. mansoni adult worms and tissue egg burden was attained. Reduced liver histopathology and granuloma size was... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_65910014" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">h i g h l i g h t s Combination of Sm14 and Sm29 antigens was tested in S. mansoni infection model. Significant reduction of S. mansoni adult worms and tissue egg burden was attained. Reduced liver histopathology and granuloma size was also achieved. Combining antigens is a realistic approach for vaccine development against S. mansoni.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/65910014" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e9b103fbbf7c8957066fb34be1d3ea75" rel="nofollow" data-download="{"attachment_id":77303734,"asset_id":65910014,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77303734/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41990123" href="https://independent.academia.edu/ShereenMossallam">Shereen Mossallam</a><script data-card-contents-for-user="41990123" type="text/json">{"id":41990123,"first_name":"Shereen","last_name":"Mossallam","domain_name":"independent","page_name":"ShereenMossallam","display_name":"Shereen Mossallam","profile_url":"https://independent.academia.edu/ShereenMossallam?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_65910014 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="65910014"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 65910014, container: ".js-paper-rank-work_65910014", }); });</script></li><li class="js-percentile-work_65910014 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 65910014; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_65910014"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_65910014 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="65910014"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 65910014; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=65910014]").text(description); $(".js-view-count-work_65910014").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_65910014").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="65910014"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4365" href="https://www.academia.edu/Documents/in/Vaccines">Vaccines</a>, <script data-card-contents-for-ri="4365" type="text/json">{"id":4365,"name":"Vaccines","url":"https://www.academia.edu/Documents/in/Vaccines?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a><script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=65910014]'), work: {"id":65910014,"title":"Combination of the two schistosomal antigens Sm14 and Sm29 elicits significant protection against experimental Schistosoma mansoni infection","created_at":"2021-12-24T22:16:51.949-08:00","url":"https://www.academia.edu/65910014/Combination_of_the_two_schistosomal_antigens_Sm14_and_Sm29_elicits_significant_protection_against_experimental_Schistosoma_mansoni_infection?f_ri=1716403","dom_id":"work_65910014","summary":"h i g h l i g h t s Combination of Sm14 and Sm29 antigens was tested in S. mansoni infection model. Significant reduction of S. mansoni adult worms and tissue egg burden was attained. Reduced liver histopathology and granuloma size was also achieved. Combining antigens is a realistic approach for vaccine development against S. mansoni.","downloadable_attachments":[{"id":77303734,"asset_id":65910014,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41990123,"first_name":"Shereen","last_name":"Mossallam","domain_name":"independent","page_name":"ShereenMossallam","display_name":"Shereen Mossallam","profile_url":"https://independent.academia.edu/ShereenMossallam?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1716403","nofollow":false},{"id":4365,"name":"Vaccines","url":"https://www.academia.edu/Documents/in/Vaccines?f_ri=1716403","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1716403","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1716403","nofollow":false},{"id":53307,"name":"Experimental parasitology","url":"https://www.academia.edu/Documents/in/Experimental_parasitology?f_ri=1716403"},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=1716403"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines?f_ri=1716403"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning?f_ri=1716403"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences?f_ri=1716403"},{"id":777305,"name":"Schistosoma Mansoni","url":"https://www.academia.edu/Documents/in/Schistosoma_Mansoni?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation?f_ri=1716403"},{"id":2734701,"name":"Biomphalaria","url":"https://www.academia.edu/Documents/in/Biomphalaria?f_ri=1716403"},{"id":3214915,"name":"Cricetinae","url":"https://www.academia.edu/Documents/in/Cricetinae?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62961392" data-work_id="62961392" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62961392/Tandem_Immobilized_Metal_Ion_Affinity_Chromatography_Immunoaffinity_Purification_of_His_tagged_Proteins_Evaluation_of_Two_Anti_His_Tag_Monoclonal_Antibodies">Tandem Immobilized Metal-Ion Affinity Chromatography/Immunoaffinity Purification of His-tagged Proteins— Evaluation of Two Anti-His-Tag Monoclonal Antibodies</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A tag comprising four to six histidines genetically fused to the protein of interest (His-tag) has been widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). Here we report the utilization of the same tag... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_62961392" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A tag comprising four to six histidines genetically fused to the protein of interest (His-tag) has been widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). Here we report the utilization of the same tag twice in series, first for IMAC and subsequently for immunoaffinity purification. Both steps are based on completely different physical principles and can therefore remove different contaminants. Two anti-His-tag antibodies (3D5 and PentaHis) were characterized for their binding and elution properties using the BIAcore surface plasmon resonance biosensor. The dissociation constant of the PentaHis antibody was determined to be 1 ؋ 10 ؊8 M and for the 3D5 antibody 3.4 ؋ 10 ؊7 M at pH 7.4. Imidazole in the sample did interfere with binding, whereas chelating agents such as EDTA and high salt did not. The antibody 3D5 was coupled to a column matrix and used for a coupled two-step purification, in which the IMAC column is eluted with EDTA and the eluent is loaded directly on the immunoaffinity column. This method may constitute a very general procedure to purify proteins to near homogeneity without the need to tailor conditions individually, and it may thus be very attractive for high-throughput screening programs and for developing general protocols for clinical grade material.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62961392" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f26704bef60fa4ebc6f73ae2c6492325" rel="nofollow" data-download="{"attachment_id":75553763,"asset_id":62961392,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75553763/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38454442" href="https://uni-potsdam.academia.edu/KatjaArndt">Katja Arndt</a><script data-card-contents-for-user="38454442" type="text/json">{"id":38454442,"first_name":"Katja","last_name":"Arndt","domain_name":"uni-potsdam","page_name":"KatjaArndt","display_name":"Katja Arndt","profile_url":"https://uni-potsdam.academia.edu/KatjaArndt?f_ri=1716403","photo":"https://0.academia-photos.com/38454442/32565879/29388339/s65_katja.arndt.jpg"}</script></span></span></li><li class="js-paper-rank-work_62961392 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="62961392"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 62961392, container: ".js-paper-rank-work_62961392", }); 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Here we report the utilization of the same tag twice in series, first for IMAC and subsequently for immunoaffinity purification. Both steps are based on completely different physical principles and can therefore remove different contaminants. Two anti-His-tag antibodies (3D5 and PentaHis) were characterized for their binding and elution properties using the BIAcore surface plasmon resonance biosensor. The dissociation constant of the PentaHis antibody was determined to be 1 ؋ 10 ؊8 M and for the 3D5 antibody 3.4 ؋ 10 ؊7 M at pH 7.4. Imidazole in the sample did interfere with binding, whereas chelating agents such as EDTA and high salt did not. The antibody 3D5 was coupled to a column matrix and used for a coupled two-step purification, in which the IMAC column is eluted with EDTA and the eluent is loaded directly on the immunoaffinity column. This method may constitute a very general procedure to purify proteins to near homogeneity without the need to tailor conditions individually, and it may thus be very attractive for high-throughput screening programs and for developing general protocols for clinical grade material.","downloadable_attachments":[{"id":75553763,"asset_id":62961392,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38454442,"first_name":"Katja","last_name":"Arndt","domain_name":"uni-potsdam","page_name":"KatjaArndt","display_name":"Katja Arndt","profile_url":"https://uni-potsdam.academia.edu/KatjaArndt?f_ri=1716403","photo":"https://0.academia-photos.com/38454442/32565879/29388339/s65_katja.arndt.jpg"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry?f_ri=1716403","nofollow":false},{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=1716403","nofollow":false},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=1716403","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403"},{"id":88745,"name":"High throughput screening","url":"https://www.academia.edu/Documents/in/High_throughput_screening?f_ri=1716403"},{"id":201241,"name":"Affinity chromatography","url":"https://www.academia.edu/Documents/in/Affinity_chromatography?f_ri=1716403"},{"id":255058,"name":"Metals","url":"https://www.academia.edu/Documents/in/Metals?f_ri=1716403"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight?f_ri=1716403"},{"id":806387,"name":"Analytical Biochemistry","url":"https://www.academia.edu/Documents/in/Analytical_Biochemistry?f_ri=1716403"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=1716403"},{"id":1365896,"name":"Histidine","url":"https://www.academia.edu/Documents/in/Histidine?f_ri=1716403"},{"id":1384644,"name":"Immunoaffinity Chromatography","url":"https://www.academia.edu/Documents/in/Immunoaffinity_Chromatography?f_ri=1716403"},{"id":1451595,"name":"Edetic Acid","url":"https://www.academia.edu/Documents/in/Edetic_Acid?f_ri=1716403"},{"id":1453163,"name":"Dissociation Constant","url":"https://www.academia.edu/Documents/in/Dissociation_Constant?f_ri=1716403"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=1716403"},{"id":2845067,"name":"High Salt","url":"https://www.academia.edu/Documents/in/High_Salt?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48188843" data-work_id="48188843" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48188843/Immunogenicity_and_cross_reactivity_against_Mycobacterium_tuberculosis_of_proteoliposomes_derived_from_Mycobacterium_bovis_BCG">Immunogenicity and cross-reactivity against Mycobacterium tuberculosis of proteoliposomes derived from Mycobacterium bovis BCG</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The only currently available vaccine against tuberculosis (TB) is Mycobacterium bovis Bacille Calmette-Guerin (BCG), which has inconsistent efficacy to protect against the disease in adults. M. tuberculosis (MTB) cell wall components have... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48188843" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The only currently available vaccine against tuberculosis (TB) is Mycobacterium bovis Bacille Calmette-Guerin (BCG), which has inconsistent efficacy to protect against the disease in adults. M. tuberculosis (MTB) cell wall components have been implicated in the pathogenicity of TB and therefore have been a prime target for the identification and characterization of cell wall proteins with potential application in vaccine development. In this regard, proteoliposomes (PLs) derived from mycobacteria containing lipids and cell wall proteins could be potential vaccine candidates against TB. In the present study PLs derived from BCG were prepared. These homogeneous population of spherical microparticles was then immunized into Balb/c mice. Sera of immunized animals showed high IgG response and strong cross-reactivity against different MTB antigens.These results showed that BCG PLs could be potential vaccine candidates against TB.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48188843" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7cf7cd5fc714ca7ece811e40d043f199" rel="nofollow" data-download="{"attachment_id":66931062,"asset_id":48188843,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66931062/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="14476740" href="https://uh-cu.academia.edu/ReinierBorrero">Reinier Borrero</a><script data-card-contents-for-user="14476740" type="text/json">{"id":14476740,"first_name":"Reinier","last_name":"Borrero","domain_name":"uh-cu","page_name":"ReinierBorrero","display_name":"Reinier Borrero","profile_url":"https://uh-cu.academia.edu/ReinierBorrero?f_ri=1716403","photo":"https://0.academia-photos.com/14476740/3946112/4612675/s65_reinier.borrero.jpg"}</script></span></span></li><li class="js-paper-rank-work_48188843 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48188843"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48188843, container: ".js-paper-rank-work_48188843", }); 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$(".js-view-count[data-work-id=48188843]").text(description); $(".js-view-count-work_48188843").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48188843").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48188843"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62235" href="https://www.academia.edu/Documents/in/Tuberculosis">Tuberculosis</a>, <script data-card-contents-for-ri="62235" type="text/json">{"id":62235,"name":"Tuberculosis","url":"https://www.academia.edu/Documents/in/Tuberculosis?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="68318" href="https://www.academia.edu/Documents/in/Mycobacterium_tuberculosis">Mycobacterium tuberculosis</a>, <script data-card-contents-for-ri="68318" type="text/json">{"id":68318,"name":"Mycobacterium tuberculosis","url":"https://www.academia.edu/Documents/in/Mycobacterium_tuberculosis?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a><script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48188843]'), work: {"id":48188843,"title":"Immunogenicity and cross-reactivity against Mycobacterium tuberculosis of proteoliposomes derived from Mycobacterium bovis BCG","created_at":"2021-05-04T04:10:06.510-07:00","url":"https://www.academia.edu/48188843/Immunogenicity_and_cross_reactivity_against_Mycobacterium_tuberculosis_of_proteoliposomes_derived_from_Mycobacterium_bovis_BCG?f_ri=1716403","dom_id":"work_48188843","summary":"The only currently available vaccine against tuberculosis (TB) is Mycobacterium bovis Bacille Calmette-Guerin (BCG), which has inconsistent efficacy to protect against the disease in adults. M. tuberculosis (MTB) cell wall components have been implicated in the pathogenicity of TB and therefore have been a prime target for the identification and characterization of cell wall proteins with potential application in vaccine development. In this regard, proteoliposomes (PLs) derived from mycobacteria containing lipids and cell wall proteins could be potential vaccine candidates against TB. In the present study PLs derived from BCG were prepared. These homogeneous population of spherical microparticles was then immunized into Balb/c mice. Sera of immunized animals showed high IgG response and strong cross-reactivity against different MTB antigens.These results showed that BCG PLs could be potential vaccine candidates against TB.","downloadable_attachments":[{"id":66931062,"asset_id":48188843,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":14476740,"first_name":"Reinier","last_name":"Borrero","domain_name":"uh-cu","page_name":"ReinierBorrero","display_name":"Reinier Borrero","profile_url":"https://uh-cu.academia.edu/ReinierBorrero?f_ri=1716403","photo":"https://0.academia-photos.com/14476740/3946112/4612675/s65_reinier.borrero.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":62235,"name":"Tuberculosis","url":"https://www.academia.edu/Documents/in/Tuberculosis?f_ri=1716403","nofollow":false},{"id":68318,"name":"Mycobacterium tuberculosis","url":"https://www.academia.edu/Documents/in/Mycobacterium_tuberculosis?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false},{"id":154672,"name":"Membrane Lipids","url":"https://www.academia.edu/Documents/in/Membrane_Lipids?f_ri=1716403"},{"id":837135,"name":"Cell Wall","url":"https://www.academia.edu/Documents/in/Cell_Wall?f_ri=1716403"},{"id":1141665,"name":"Mycobacterium bovis","url":"https://www.academia.edu/Documents/in/Mycobacterium_bovis?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2212745,"name":"BCG vaccine","url":"https://www.academia.edu/Documents/in/BCG_vaccine?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_29439902" data-work_id="29439902" itemscope="itemscope" 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}); });</script></span><script>$(function() { $(".js-view-count-work_29439902").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="29439902"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="173" href="https://www.academia.edu/Documents/in/Zoology">Zoology</a>, <script data-card-contents-for-ri="173" type="text/json">{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="74780" href="https://www.academia.edu/Documents/in/Mutation">Mutation</a>, <script data-card-contents-for-ri="74780" type="text/json">{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a><script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=29439902]'), work: {"id":29439902,"title":"Comparison of Different Attenuation Strategies in Development of a Salmonella hadar Vaccine","created_at":"2016-10-26T08:48:26.861-07:00","url":"https://www.academia.edu/29439902/Comparison_of_Different_Attenuation_Strategies_in_Development_of_a_Salmonella_hadar_Vaccine?f_ri=1716403","dom_id":"work_29439902","summary":null,"downloadable_attachments":[{"id":49885670,"asset_id":29439902,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":49628001,"first_name":"Donata","last_name":"Sizemore","domain_name":"independent","page_name":"DonataSizemore","display_name":"Donata Sizemore","profile_url":"https://independent.academia.edu/DonataSizemore?f_ri=1716403","photo":"https://0.academia-photos.com/49628001/17869036/17888287/s65_donata.sizemore.jpeg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1716403","nofollow":false},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=1716403","nofollow":false},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation?f_ri=1716403","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=1716403","nofollow":false},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids?f_ri=1716403"},{"id":257897,"name":"Salmonella","url":"https://www.academia.edu/Documents/in/Salmonella?f_ri=1716403"},{"id":366620,"name":"POULTRY DISEASES","url":"https://www.academia.edu/Documents/in/POULTRY_DISEASES?f_ri=1716403"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens?f_ri=1716403"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences?f_ri=1716403"},{"id":770676,"name":"Avian Diseases","url":"https://www.academia.edu/Documents/in/Avian_Diseases?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5649971" data-work_id="5649971" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5649971/Immunoglobulin_producing_tumours_in_dogs_and_cats">Immunoglobulin-producing tumours in dogs and cats</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Turnouts with a plasmacytoid pattern taken from 32 dogs and finn" cats were examined for the presence Jimmunoglobulins, which would allow them to be designated as B-cell Iymphomas. Within a total ot" 19 immunogIobulinpositive turnouts,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5649971" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Turnouts with a plasmacytoid pattern taken from 32 dogs and finn" cats were examined for the presence Jimmunoglobulins, which would allow them to be designated as B-cell Iymphomas. Within a total ot" 19 immunogIobulinpositive turnouts, three types could be distinguished: extramedullary plasmacytoma (15), multiple myeloma (two) and immunocytoma (two). These tumours occurred in 18 of" the dogs, and in one cat (extramedullary plasmacytoma). The characteristics o[" the immunoglobulin-producing tumours were investigated by light and electron-mlcroscopy as well as by immunohistochemical rnethods. Seventeen of the l g tumom's expressed ~.-type light chains and one turnout K-type light chains. Heavy chains were also synthesized by five tuinoul's.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5649971" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="944f67e20c6ea70fc8c11200e0df771a" rel="nofollow" data-download="{"attachment_id":49197788,"asset_id":5649971,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49197788/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="8129176" href="https://independent.academia.edu/StefanPlatz">Stefan Platz</a><script data-card-contents-for-user="8129176" type="text/json">{"id":8129176,"first_name":"Stefan","last_name":"Platz","domain_name":"independent","page_name":"StefanPlatz","display_name":"Stefan Platz","profile_url":"https://independent.academia.edu/StefanPlatz?f_ri=1716403","photo":"https://0.academia-photos.com/8129176/172474979/162478435/s65_stefan.platz.png"}</script></span></span></li><li class="js-paper-rank-work_5649971 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5649971"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5649971, container: ".js-paper-rank-work_5649971", }); });</script></li><li class="js-percentile-work_5649971 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 5649971; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_5649971"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_5649971 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="5649971"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 5649971; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=5649971]").text(description); $(".js-view-count-work_5649971").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_5649971").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="5649971"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2184" href="https://www.academia.edu/Documents/in/Electron_Microscopy">Electron Microscopy</a>, <script data-card-contents-for-ri="2184" type="text/json">{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="33208" href="https://www.academia.edu/Documents/in/Comparative">Comparative</a>, <script data-card-contents-for-ri="33208" type="text/json">{"id":33208,"name":"Comparative","url":"https://www.academia.edu/Documents/in/Comparative?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="41088" href="https://www.academia.edu/Documents/in/Cats">Cats</a><script data-card-contents-for-ri="41088" type="text/json">{"id":41088,"name":"Cats","url":"https://www.academia.edu/Documents/in/Cats?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=5649971]'), work: {"id":5649971,"title":"Immunoglobulin-producing tumours in dogs and cats","created_at":"2014-01-08T17:35:41.687-08:00","url":"https://www.academia.edu/5649971/Immunoglobulin_producing_tumours_in_dogs_and_cats?f_ri=1716403","dom_id":"work_5649971","summary":"Turnouts with a plasmacytoid pattern taken from 32 dogs and finn\" cats were examined for the presence Jimmunoglobulins, which would allow them to be designated as B-cell Iymphomas. Within a total ot\" 19 immunogIobulinpositive turnouts, three types could be distinguished: extramedullary plasmacytoma (15), multiple myeloma (two) and immunocytoma (two). These tumours occurred in 18 of\" the dogs, and in one cat (extramedullary plasmacytoma). The characteristics o[\" the immunoglobulin-producing tumours were investigated by light and electron-mlcroscopy as well as by immunohistochemical rnethods. Seventeen of the l g tumom's expressed ~.-type light chains and one turnout K-type light chains. Heavy chains were also synthesized by five tuinoul's.","downloadable_attachments":[{"id":49197788,"asset_id":5649971,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":8129176,"first_name":"Stefan","last_name":"Platz","domain_name":"independent","page_name":"StefanPlatz","display_name":"Stefan Platz","profile_url":"https://independent.academia.edu/StefanPlatz?f_ri=1716403","photo":"https://0.academia-photos.com/8129176/172474979/162478435/s65_stefan.platz.png"}],"research_interests":[{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=1716403","nofollow":false},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=1716403","nofollow":false},{"id":33208,"name":"Comparative","url":"https://www.academia.edu/Documents/in/Comparative?f_ri=1716403","nofollow":false},{"id":41088,"name":"Cats","url":"https://www.academia.edu/Documents/in/Cats?f_ri=1716403","nofollow":false},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs?f_ri=1716403"},{"id":111110,"name":"Multiple Myeloma","url":"https://www.academia.edu/Documents/in/Multiple_Myeloma?f_ri=1716403"},{"id":541328,"name":"Diffuse Large B-Cell Lymphoma","url":"https://www.academia.edu/Documents/in/Diffuse_Large_B-Cell_Lymphoma?f_ri=1716403"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences?f_ri=1716403"},{"id":1312086,"name":"Immunoglobulins","url":"https://www.academia.edu/Documents/in/Immunoglobulins?f_ri=1716403"},{"id":1426712,"name":"Immunoglobulin","url":"https://www.academia.edu/Documents/in/Immunoglobulin?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2483297,"name":"Immunoglobulin A","url":"https://www.academia.edu/Documents/in/Immunoglobulin_A?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6322137" data-work_id="6322137" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6322137/Virus_inactivation_by_pepsin_treatment_at_pH_4_of_IgG_solutions_factors_affecting_the_rate_of_virus_inactivation">Virus inactivation by pepsin treatment at pH 4 of IgG solutions: factors affecting the rate of virus inactivation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: IgG preparations have rarely transmitted infectious diseases; however, because such transmission has occurred a few times, manufacturers are required to present experimental proof that their specific production process removes... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6322137" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: IgG preparations have rarely transmitted infectious diseases; however, because such transmission has occurred a few times, manufacturers are required to present experimental proof that their specific production process removes and/or inactivates viruses that may be present in the starting material. Study Design and Methods: The kinetics of virus inactivation mediated by pepsin treatment at pH 4 during the production of intravenous immunoglobulin was assessed with spiking experiments using human immunodeficiency virus, bovine viral diarrhea virus, Semliki Forest virus, and pseudorabies virus. The influence of various factors on the rate of virus inactivation also was studied by modifying the composition of the IgG solutions with respect to IgG, sucrose, and NaCl content. Results: Virus inactivation at 37°C was extremely rapid and resulted in a complete loss of infectivity within 5 minutes to 1 hour. Inactivation was much slower at lower temperatures. Furthermore, inactivation was dependent on the solute composition. Increasing the sucrose content from 0 to 15 percent reduced the rate of inactivation of pseudorabies virus but did not affect the rate of inactivation of Semliki Forest virus. In contrast, increasing the NaCl content from 0 to 150 mM resulted in a reduction in the rate of inactivation of Semliki Forest virus, whereas the rate of inactivation of pseudorabies virus remained unaffected. Moreover, increasing the IgG concentration from 0 to 10 percent resulted in an increased rate of inactivation of pseudorabies virus but a decreased rate of inactivation of Semliki Forest virus. Conclusion: Inactivation of viruses by pepsin treatment at pH 4 essentially is temperature-dependent, and the reaction rate is selectively influenced by the solute composition of the IgG solution. This has to be taken into account when safety data for different products are compared. Abbreviations: BVDV = bovine viral diarrhea virus; HCV = hepatitis C virus; HIV = human immunodeficiency virus; pH Upepsin = pepsin treatment at pH 4; PRV = pseudorabies virus; SFV = Semliki Forest virus; TCID,, = median tissue culture-Infective doses.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6322137" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="587effa57830c32ad55d07f7bf590495" rel="nofollow" data-download="{"attachment_id":48917768,"asset_id":6322137,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48917768/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="9831816" href="https://independent.academia.edu/kempfc">christoph kempf</a><script data-card-contents-for-user="9831816" type="text/json">{"id":9831816,"first_name":"christoph","last_name":"kempf","domain_name":"independent","page_name":"kempfc","display_name":"christoph kempf","profile_url":"https://independent.academia.edu/kempfc?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_6322137 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6322137"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6322137, container: ".js-paper-rank-work_6322137", }); });</script></li><li class="js-percentile-work_6322137 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 6322137; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_6322137"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_6322137 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="6322137"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6322137; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=6322137]").text(description); $(".js-view-count-work_6322137").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6322137").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6322137"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39978" href="https://www.academia.edu/Documents/in/HIV">HIV</a>, <script data-card-contents-for-ri="39978" type="text/json">{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="133177" href="https://www.academia.edu/Documents/in/Temperature">Temperature</a>, <script data-card-contents-for-ri="133177" type="text/json">{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="244814" href="https://www.academia.edu/Documents/in/Clinical_Sciences">Clinical Sciences</a><script data-card-contents-for-ri="244814" type="text/json">{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6322137]'), work: {"id":6322137,"title":"Virus inactivation by pepsin treatment at pH 4 of IgG solutions: factors affecting the rate of virus inactivation","created_at":"2014-03-06T23:34:53.423-08:00","url":"https://www.academia.edu/6322137/Virus_inactivation_by_pepsin_treatment_at_pH_4_of_IgG_solutions_factors_affecting_the_rate_of_virus_inactivation?f_ri=1716403","dom_id":"work_6322137","summary":"Background: IgG preparations have rarely transmitted infectious diseases; however, because such transmission has occurred a few times, manufacturers are required to present experimental proof that their specific production process removes and/or inactivates viruses that may be present in the starting material. Study Design and Methods: The kinetics of virus inactivation mediated by pepsin treatment at pH 4 during the production of intravenous immunoglobulin was assessed with spiking experiments using human immunodeficiency virus, bovine viral diarrhea virus, Semliki Forest virus, and pseudorabies virus. The influence of various factors on the rate of virus inactivation also was studied by modifying the composition of the IgG solutions with respect to IgG, sucrose, and NaCl content. Results: Virus inactivation at 37°C was extremely rapid and resulted in a complete loss of infectivity within 5 minutes to 1 hour. Inactivation was much slower at lower temperatures. Furthermore, inactivation was dependent on the solute composition. Increasing the sucrose content from 0 to 15 percent reduced the rate of inactivation of pseudorabies virus but did not affect the rate of inactivation of Semliki Forest virus. In contrast, increasing the NaCl content from 0 to 150 mM resulted in a reduction in the rate of inactivation of Semliki Forest virus, whereas the rate of inactivation of pseudorabies virus remained unaffected. Moreover, increasing the IgG concentration from 0 to 10 percent resulted in an increased rate of inactivation of pseudorabies virus but a decreased rate of inactivation of Semliki Forest virus. Conclusion: Inactivation of viruses by pepsin treatment at pH 4 essentially is temperature-dependent, and the reaction rate is selectively influenced by the solute composition of the IgG solution. This has to be taken into account when safety data for different products are compared. Abbreviations: BVDV = bovine viral diarrhea virus; HCV = hepatitis C virus; HIV = human immunodeficiency virus; pH Upepsin = pepsin treatment at pH 4; PRV = pseudorabies virus; SFV = Semliki Forest virus; TCID,, = median tissue culture-Infective doses.","downloadable_attachments":[{"id":48917768,"asset_id":6322137,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":9831816,"first_name":"christoph","last_name":"kempf","domain_name":"independent","page_name":"kempfc","display_name":"christoph kempf","profile_url":"https://independent.academia.edu/kempfc?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV?f_ri=1716403","nofollow":false},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1716403","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403","nofollow":false},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=1716403"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=1716403"},{"id":1307860,"name":"Transfusion","url":"https://www.academia.edu/Documents/in/Transfusion?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6739370" data-work_id="6739370" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6739370/The_majority_of_natural_immunoglobulin_secreting_cells_are_short_lived_and_the_progeny_of_cycling_lymphocytes">The majority of “natural” immunoglobulin-secreting cells are short-lived and the progeny of cycling lymphocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Treatment of mice with hydroxyurea to selectively kill all cycling cells has been used to study population dynamics and life expectancy of “natural” immunoglobulin-secreting cells in the bone marrow and spleen of nonimmunized animals. The... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6739370" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Treatment of mice with hydroxyurea to selectively kill all cycling cells has been used to study population dynamics and life expectancy of “natural” immunoglobulin-secreting cells in the bone marrow and spleen of nonimmunized animals. The results show that 50 to 90% of those cells are eliminated 2 to 3 days after one cycle of hydroxyurea administration, demonstrating their recent origin from cycling precursors. Using a protocol of long-term hydroxyurea treatment which abrogates cell production from the cycling precursors compartments, it was shown that “natural” immunoglobulin-secreting cells have a very short half renewal time, in the range of 15–60 h.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6739370" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="61d894b2be46eb23dc25bc5d28497ec6" rel="nofollow" data-download="{"attachment_id":48738456,"asset_id":6739370,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48738456/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="11076299" href="https://independent.academia.edu/Ant%C3%B3nioFreitas4">António Freitas</a><script data-card-contents-for-user="11076299" type="text/json">{"id":11076299,"first_name":"António","last_name":"Freitas","domain_name":"independent","page_name":"AntónioFreitas4","display_name":"António Freitas","profile_url":"https://independent.academia.edu/Ant%C3%B3nioFreitas4?f_ri=1716403","photo":"https://0.academia-photos.com/11076299/3869864/4526730/s65_ant_nio.freitas.jpg"}</script></span></span></li><li class="js-paper-rank-work_6739370 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6739370"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6739370, container: ".js-paper-rank-work_6739370", }); });</script></li><li class="js-percentile-work_6739370 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 6739370; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_6739370"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_6739370 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="6739370"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6739370; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=6739370]").text(description); $(".js-view-count-work_6739370").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6739370").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6739370"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="3642" href="https://www.academia.edu/Documents/in/Stem_Cells">Stem Cells</a>, <script data-card-contents-for-ri="3642" type="text/json">{"id":3642,"name":"Stem Cells","url":"https://www.academia.edu/Documents/in/Stem_Cells?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6791" href="https://www.academia.edu/Documents/in/Aging">Aging</a>, <script data-card-contents-for-ri="6791" type="text/json">{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9113" href="https://www.academia.edu/Documents/in/Cell_Cycle">Cell Cycle</a><script data-card-contents-for-ri="9113" type="text/json">{"id":9113,"name":"Cell Cycle","url":"https://www.academia.edu/Documents/in/Cell_Cycle?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6739370]'), work: {"id":6739370,"title":"The majority of “natural” immunoglobulin-secreting cells are short-lived and the progeny of cycling lymphocytes","created_at":"2014-04-12T20:05:58.701-07:00","url":"https://www.academia.edu/6739370/The_majority_of_natural_immunoglobulin_secreting_cells_are_short_lived_and_the_progeny_of_cycling_lymphocytes?f_ri=1716403","dom_id":"work_6739370","summary":"Treatment of mice with hydroxyurea to selectively kill all cycling cells has been used to study population dynamics and life expectancy of “natural” immunoglobulin-secreting cells in the bone marrow and spleen of nonimmunized animals. The results show that 50 to 90% of those cells are eliminated 2 to 3 days after one cycle of hydroxyurea administration, demonstrating their recent origin from cycling precursors. Using a protocol of long-term hydroxyurea treatment which abrogates cell production from the cycling precursors compartments, it was shown that “natural” immunoglobulin-secreting cells have a very short half renewal time, in the range of 15–60 h.","downloadable_attachments":[{"id":48738456,"asset_id":6739370,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":11076299,"first_name":"António","last_name":"Freitas","domain_name":"independent","page_name":"AntónioFreitas4","display_name":"António Freitas","profile_url":"https://independent.academia.edu/Ant%C3%B3nioFreitas4?f_ri=1716403","photo":"https://0.academia-photos.com/11076299/3869864/4526730/s65_ant_nio.freitas.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":3642,"name":"Stem Cells","url":"https://www.academia.edu/Documents/in/Stem_Cells?f_ri=1716403","nofollow":false},{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=1716403","nofollow":false},{"id":9113,"name":"Cell Cycle","url":"https://www.academia.edu/Documents/in/Cell_Cycle?f_ri=1716403","nofollow":false},{"id":49018,"name":"Spleen","url":"https://www.academia.edu/Documents/in/Spleen?f_ri=1716403"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403"},{"id":279027,"name":"European","url":"https://www.academia.edu/Documents/in/European?f_ri=1716403"},{"id":678853,"name":"B Lymphocytes","url":"https://www.academia.edu/Documents/in/B_Lymphocytes?f_ri=1716403"},{"id":731153,"name":"Hydroxyurea","url":"https://www.academia.edu/Documents/in/Hydroxyurea?f_ri=1716403"},{"id":868560,"name":"Lymphocytes","url":"https://www.academia.edu/Documents/in/Lymphocytes?f_ri=1716403"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival?f_ri=1716403"},{"id":1426712,"name":"Immunoglobulin","url":"https://www.academia.edu/Documents/in/Immunoglobulin?f_ri=1716403"},{"id":1664707,"name":"Bone Marrow Cells","url":"https://www.academia.edu/Documents/in/Bone_Marrow_Cells?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1757315,"name":"Injections","url":"https://www.academia.edu/Documents/in/Injections?f_ri=1716403"},{"id":2079216,"name":"Lymph nodes","url":"https://www.academia.edu/Documents/in/Lymph_nodes?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_7847420" data-work_id="7847420" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/7847420/Specificity_of_IgG_and_IgE_antibody_responses_to_Haemophilus_somnus_infection_of_calves">Specificity of IgG and IgE antibody responses to Haemophilus somnus infection of calves</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Haemophilus somnus is an important cause of bovine respiratory disease and septicemia with all it's sequelae. The role of immune responses in protection and immunopathogenesis is not well understood. We showed that infection with bovine... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_7847420" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Haemophilus somnus is an important cause of bovine respiratory disease and septicemia with all it's sequelae. The role of immune responses in protection and immunopathogenesis is not well understood. We showed that infection with bovine respiratory syncytial virus (BRSV) 6 days before H. somnus increased clinical scores and levels of IgE antibody to H. somnus over that of infection with H. somnus alone. To determine whether antigenic specificity of IgE responses differed from IgG responses, Western blots were done with sera from the infected calves, at 0 time and at 21 days post infection. Thus each calf was its own control. IgG antibodies recognized primarily a 40 kDa outer membrane protein (OMP) in whole cell H. somnus preparations and a 270 kDa immunoglobulin binding protein (IgBPs) in culture supernatants but generally not the 41 kDa major OMP (MOMP). IgE antibodies recognized primarily the 41 kDa MOMP in whole cell pellet preparations. Results were consistent among calves. With culture supernatants, IgE antibodies recognized both the 270 kDa IgBPs and the MOMP. Since some H. somnus strains from asymptomatic carriers (including strain 129Pt), do not have IgBPs and express a truncated MOMP (33 kDa rather than 41 kDa), reaction of strain 129Pt cells with serum from calves infected with H. somnus or BRSV and H. somnus was studied. IgE did not react with the truncated MOMP even at much lower (1:100) dilutions than in Western blots with virulent strain 2336 (serum dilution of 1:500). Reactions of IgE with the 40 and 78 kDa antigens in strain 129Pt were noted but since the major reactivities with the IgBPs and the MOMP were not detected, this strain may be useful for inducing protective rather than immunopathogenic responses. #</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/7847420" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f0d3f9074410222efc02536a5be43342" rel="nofollow" data-download="{"attachment_id":48317807,"asset_id":7847420,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48317807/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="14607673" href="https://independent.academia.edu/KimballRichard">Richard Kimball</a><script data-card-contents-for-user="14607673" type="text/json">{"id":14607673,"first_name":"Richard","last_name":"Kimball","domain_name":"independent","page_name":"KimballRichard","display_name":"Richard Kimball","profile_url":"https://independent.academia.edu/KimballRichard?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_7847420 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="7847420"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 7847420, container: ".js-paper-rank-work_7847420", }); });</script></li><li class="js-percentile-work_7847420 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 7847420; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_7847420"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_7847420 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="7847420"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 7847420; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=7847420]").text(description); $(".js-view-count-work_7847420").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_7847420").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="7847420"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="173" href="https://www.academia.edu/Documents/in/Zoology">Zoology</a>, <script data-card-contents-for-ri="173" type="text/json">{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2702" href="https://www.academia.edu/Documents/in/Immune_response">Immune response</a>, <script data-card-contents-for-ri="2702" type="text/json">{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="37834" href="https://www.academia.edu/Documents/in/Western_blotting">Western blotting</a>, <script data-card-contents-for-ri="37834" type="text/json">{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="176180" href="https://www.academia.edu/Documents/in/Immunoglobulin_E">Immunoglobulin E</a><script data-card-contents-for-ri="176180" type="text/json">{"id":176180,"name":"Immunoglobulin E","url":"https://www.academia.edu/Documents/in/Immunoglobulin_E?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=7847420]'), work: {"id":7847420,"title":"Specificity of IgG and IgE antibody responses to Haemophilus somnus infection of calves","created_at":"2014-08-02T00:58:47.637-07:00","url":"https://www.academia.edu/7847420/Specificity_of_IgG_and_IgE_antibody_responses_to_Haemophilus_somnus_infection_of_calves?f_ri=1716403","dom_id":"work_7847420","summary":"Haemophilus somnus is an important cause of bovine respiratory disease and septicemia with all it's sequelae. The role of immune responses in protection and immunopathogenesis is not well understood. We showed that infection with bovine respiratory syncytial virus (BRSV) 6 days before H. somnus increased clinical scores and levels of IgE antibody to H. somnus over that of infection with H. somnus alone. To determine whether antigenic specificity of IgE responses differed from IgG responses, Western blots were done with sera from the infected calves, at 0 time and at 21 days post infection. Thus each calf was its own control. IgG antibodies recognized primarily a 40 kDa outer membrane protein (OMP) in whole cell H. somnus preparations and a 270 kDa immunoglobulin binding protein (IgBPs) in culture supernatants but generally not the 41 kDa major OMP (MOMP). IgE antibodies recognized primarily the 41 kDa MOMP in whole cell pellet preparations. Results were consistent among calves. With culture supernatants, IgE antibodies recognized both the 270 kDa IgBPs and the MOMP. Since some H. somnus strains from asymptomatic carriers (including strain 129Pt), do not have IgBPs and express a truncated MOMP (33 kDa rather than 41 kDa), reaction of strain 129Pt cells with serum from calves infected with H. somnus or BRSV and H. somnus was studied. IgE did not react with the truncated MOMP even at much lower (1:100) dilutions than in Western blots with virulent strain 2336 (serum dilution of 1:500). Reactions of IgE with the 40 and 78 kDa antigens in strain 129Pt were noted but since the major reactivities with the IgBPs and the MOMP were not detected, this strain may be useful for inducing protective rather than immunopathogenic responses. #","downloadable_attachments":[{"id":48317807,"asset_id":7847420,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":14607673,"first_name":"Richard","last_name":"Kimball","domain_name":"independent","page_name":"KimballRichard","display_name":"Richard Kimball","profile_url":"https://independent.academia.edu/KimballRichard?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=1716403","nofollow":false},{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=1716403","nofollow":false},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=1716403","nofollow":false},{"id":176180,"name":"Immunoglobulin E","url":"https://www.academia.edu/Documents/in/Immunoglobulin_E?f_ri=1716403","nofollow":false},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=1716403"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot?f_ri=1716403"},{"id":509782,"name":"Respiratory Tract Infections","url":"https://www.academia.edu/Documents/in/Respiratory_Tract_Infections?f_ri=1716403"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences?f_ri=1716403"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding?f_ri=1716403"},{"id":1159038,"name":"Epitopes","url":"https://www.academia.edu/Documents/in/Epitopes?f_ri=1716403"},{"id":1180273,"name":"Outer Membrane Protein","url":"https://www.academia.edu/Documents/in/Outer_Membrane_Protein?f_ri=1716403"},{"id":1361057,"name":"Respiratory Syncytial Virus Infections Industry","url":"https://www.academia.edu/Documents/in/Respiratory_Syncytial_Virus_Infections_Industry?f_ri=1716403"},{"id":1426712,"name":"Immunoglobulin","url":"https://www.academia.edu/Documents/in/Immunoglobulin?f_ri=1716403"},{"id":1533489,"name":"Haemophilus Infections Market","url":"https://www.academia.edu/Documents/in/Haemophilus_Infections_Market?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27321866" data-work_id="27321866" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27321866/Antibody_orientation_enhanced_by_selective_polymer_protein_noncovalent_interactions">Antibody orientation enhanced by selective polymer–protein noncovalent interactions</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A unique interaction has been found between protein G' (a truncated recombinant bacterial "alphabet" protein which aligns by noncovalent attachment to the antibody stem) and poly(methyl methacrylate), a thermoplastic polymer substrate,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_27321866" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A unique interaction has been found between protein G' (a truncated recombinant bacterial "alphabet" protein which aligns by noncovalent attachment to the antibody stem) and poly(methyl methacrylate), a thermoplastic polymer substrate, which can be easily fabricated using high-rate processes. Significantly improved orientation efficiency with traditional passive adsorption for this system (termed ALYGNSA) has been achieved as compared to the same assay performed on a polystyrene substrate with protein G'. Results were consistent with an average alignment of 80% of the human immunoglobulin G capture antibody which translated into a 30% to 50% improved alignment over an array of industry standards tested. Laser scanning confocal microscopy confirmed the immunological results. Studies of additional poly(methyl methacrylate) polymer derivatives and protein biolinker (A and AG) combinations have been conducted and have revealed different degrees of antibody alignment. These findings may lead to additional novel noncovalent methods of antibody orientation and greater sensitivity in immunological assays.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27321866" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b283300773556273f518a70a158e630a" rel="nofollow" data-download="{"attachment_id":47577902,"asset_id":27321866,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47577902/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51431890" href="https://independent.academia.edu/LisaJoClarizia">Lisa-Jo Clarizia</a><script data-card-contents-for-user="51431890" type="text/json">{"id":51431890,"first_name":"Lisa-Jo","last_name":"Clarizia","domain_name":"independent","page_name":"LisaJoClarizia","display_name":"Lisa-Jo Clarizia","profile_url":"https://independent.academia.edu/LisaJoClarizia?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_27321866 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27321866"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27321866, container: ".js-paper-rank-work_27321866", }); });</script></li><li class="js-percentile-work_27321866 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27321866; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27321866"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27321866 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27321866"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27321866; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27321866]").text(description); $(".js-view-count-work_27321866").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27321866").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27321866"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7698" href="https://www.academia.edu/Documents/in/Fluorescence">Fluorescence</a>, <script data-card-contents-for-ri="7698" type="text/json">{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="69424" href="https://www.academia.edu/Documents/in/Protein_Kinases">Protein Kinases</a><script data-card-contents-for-ri="69424" type="text/json">{"id":69424,"name":"Protein Kinases","url":"https://www.academia.edu/Documents/in/Protein_Kinases?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27321866]'), work: {"id":27321866,"title":"Antibody orientation enhanced by selective polymer–protein noncovalent interactions","created_at":"2016-07-27T18:57:33.095-07:00","url":"https://www.academia.edu/27321866/Antibody_orientation_enhanced_by_selective_polymer_protein_noncovalent_interactions?f_ri=1716403","dom_id":"work_27321866","summary":"A unique interaction has been found between protein G' (a truncated recombinant bacterial \"alphabet\" protein which aligns by noncovalent attachment to the antibody stem) and poly(methyl methacrylate), a thermoplastic polymer substrate, which can be easily fabricated using high-rate processes. Significantly improved orientation efficiency with traditional passive adsorption for this system (termed ALYGNSA) has been achieved as compared to the same assay performed on a polystyrene substrate with protein G'. Results were consistent with an average alignment of 80% of the human immunoglobulin G capture antibody which translated into a 30% to 50% improved alignment over an array of industry standards tested. Laser scanning confocal microscopy confirmed the immunological results. Studies of additional poly(methyl methacrylate) polymer derivatives and protein biolinker (A and AG) combinations have been conducted and have revealed different degrees of antibody alignment. These findings may lead to additional novel noncovalent methods of antibody orientation and greater sensitivity in immunological assays.","downloadable_attachments":[{"id":47577902,"asset_id":27321866,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51431890,"first_name":"Lisa-Jo","last_name":"Clarizia","domain_name":"independent","page_name":"LisaJoClarizia","display_name":"Lisa-Jo Clarizia","profile_url":"https://independent.academia.edu/LisaJoClarizia?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=1716403","nofollow":false},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=1716403","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false},{"id":69424,"name":"Protein Kinases","url":"https://www.academia.edu/Documents/in/Protein_Kinases?f_ri=1716403","nofollow":false},{"id":80221,"name":"Lasers","url":"https://www.academia.edu/Documents/in/Lasers?f_ri=1716403"},{"id":132569,"name":"Analytical","url":"https://www.academia.edu/Documents/in/Analytical?f_ri=1716403"},{"id":216315,"name":"Laser Scanning Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Laser_Scanning_Confocal_Microscopy?f_ri=1716403"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=1716403"},{"id":621932,"name":"Analytical and Bioanalytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_and_Bioanalytical_Chemistry?f_ri=1716403"},{"id":1391697,"name":"Polymethyl Methacrylate (PMMA)","url":"https://www.academia.edu/Documents/in/Polymethyl_Methacrylate_PMMA_?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_12959062" data-work_id="12959062" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/12959062/Removing_IgG_antibodies_from_intact_red_cells_comparison_of_acid_and_EDTA_heat_and_chloroquine_elution_methods">Removing IgG antibodies from intact red cells: comparison of acid and EDTA, heat, and chloroquine elution methods</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To accurately phenotype red cells from patients with a positive direct antiglobulin test (DAT), nonlytic elution procedures were assessed for their ability to dissociate IgG from antibody-coated red cells without altering red cell antigen... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_12959062" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To accurately phenotype red cells from patients with a positive direct antiglobulin test (DAT), nonlytic elution procedures were assessed for their ability to dissociate IgG from antibody-coated red cells without altering red cell antigen expression. STUDY DESIGN AND METHODS: Antibodies coating red cells that were sensitized in vivo (warm-reactive autoantibodies: 8 patients) or in vitro were eluted by using glycine-HCI and EDTA (acid/ EDTA), heat (56"C, 10 min), or chloroquine method. RESULTS: Acid/EDTA elution gave the best results, reducing DAT positivity to microscopic levels or rendering the DAT negative in 48 of 50 instances, whereas 4 samples remained resistant to heat elution and 24 to chloroquine. Standard DAT agglutination scores demonstrated that both acid/EDTA and heat elution were superior to the chloroquine method (p<O.OOOl). With the gel low-ionic-strength saline indirect antiglobulin test, acid/ EDTA was superior to heat (p<O.OOl). Overall, acid/ EDTA elution dissociated more antibodies than heat (p<O.OOOl), especially for Kell system (K, k, Kpa, Kpb) alloantibodies. Common red cell antigens, other than Kell system antigens, were unaffected by acid/EDTA elution. In contrast, the expression of most blood group antigens was diminished after heat elution. However, it was possible to type red cell antigens by using gel low-ionicstrength saline indirect antiglobulin tests or tube agglutination methods.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12959062" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5cb841d8c09015cb9eef5f3c22070539" rel="nofollow" data-download="{"attachment_id":45822380,"asset_id":12959062,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45822380/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32152387" href="https://independent.academia.edu/NicolasBurindesRoziers">Nicolas Burin des Roziers</a><script data-card-contents-for-user="32152387" type="text/json">{"id":32152387,"first_name":"Nicolas","last_name":"Burin des Roziers","domain_name":"independent","page_name":"NicolasBurindesRoziers","display_name":"Nicolas Burin des Roziers","profile_url":"https://independent.academia.edu/NicolasBurindesRoziers?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_12959062 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="12959062"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 12959062, container: ".js-paper-rank-work_12959062", }); 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$(".js-view-count[data-work-id=12959062]").text(description); $(".js-view-count-work_12959062").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_12959062").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="12959062"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="51789" href="https://www.academia.edu/Documents/in/Antibodies">Antibodies</a>, <script data-card-contents-for-ri="51789" type="text/json">{"id":51789,"name":"Antibodies","url":"https://www.academia.edu/Documents/in/Antibodies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="54344" href="https://www.academia.edu/Documents/in/Methods">Methods</a>, <script data-card-contents-for-ri="54344" type="text/json">{"id":54344,"name":"Methods","url":"https://www.academia.edu/Documents/in/Methods?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="244814" href="https://www.academia.edu/Documents/in/Clinical_Sciences">Clinical Sciences</a><script data-card-contents-for-ri="244814" type="text/json">{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=12959062]'), work: {"id":12959062,"title":"Removing IgG antibodies from intact red cells: comparison of acid and EDTA, heat, and chloroquine elution methods","created_at":"2015-06-13T04:29:55.562-07:00","url":"https://www.academia.edu/12959062/Removing_IgG_antibodies_from_intact_red_cells_comparison_of_acid_and_EDTA_heat_and_chloroquine_elution_methods?f_ri=1716403","dom_id":"work_12959062","summary":"To accurately phenotype red cells from patients with a positive direct antiglobulin test (DAT), nonlytic elution procedures were assessed for their ability to dissociate IgG from antibody-coated red cells without altering red cell antigen expression. STUDY DESIGN AND METHODS: Antibodies coating red cells that were sensitized in vivo (warm-reactive autoantibodies: 8 patients) or in vitro were eluted by using glycine-HCI and EDTA (acid/ EDTA), heat (56\"C, 10 min), or chloroquine method. RESULTS: Acid/EDTA elution gave the best results, reducing DAT positivity to microscopic levels or rendering the DAT negative in 48 of 50 instances, whereas 4 samples remained resistant to heat elution and 24 to chloroquine. Standard DAT agglutination scores demonstrated that both acid/EDTA and heat elution were superior to the chloroquine method (p\u003cO.OOOl). With the gel low-ionic-strength saline indirect antiglobulin test, acid/ EDTA was superior to heat (p\u003cO.OOl). Overall, acid/ EDTA elution dissociated more antibodies than heat (p\u003cO.OOOl), especially for Kell system (K, k, Kpa, Kpb) alloantibodies. Common red cell antigens, other than Kell system antigens, were unaffected by acid/EDTA elution. In contrast, the expression of most blood group antigens was diminished after heat elution. However, it was possible to type red cell antigens by using gel low-ionicstrength saline indirect antiglobulin tests or tube agglutination methods.","downloadable_attachments":[{"id":45822380,"asset_id":12959062,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32152387,"first_name":"Nicolas","last_name":"Burin des Roziers","domain_name":"independent","page_name":"NicolasBurindesRoziers","display_name":"Nicolas Burin des Roziers","profile_url":"https://independent.academia.edu/NicolasBurindesRoziers?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":51789,"name":"Antibodies","url":"https://www.academia.edu/Documents/in/Antibodies?f_ri=1716403","nofollow":false},{"id":54344,"name":"Methods","url":"https://www.academia.edu/Documents/in/Methods?f_ri=1716403","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403","nofollow":false},{"id":919274,"name":"Chloroquine","url":"https://www.academia.edu/Documents/in/Chloroquine?f_ri=1716403"},{"id":982534,"name":"Erythrocytes","url":"https://www.academia.edu/Documents/in/Erythrocytes?f_ri=1716403"},{"id":1256875,"name":"Acids","url":"https://www.academia.edu/Documents/in/Acids?f_ri=1716403"},{"id":1307860,"name":"Transfusion","url":"https://www.academia.edu/Documents/in/Transfusion?f_ri=1716403"},{"id":1451595,"name":"Edetic Acid","url":"https://www.academia.edu/Documents/in/Edetic_Acid?f_ri=1716403"},{"id":1482586,"name":"Autoantibodies","url":"https://www.academia.edu/Documents/in/Autoantibodies?f_ri=1716403"},{"id":1491514,"name":"*Hot Temperature","url":"https://www.academia.edu/Documents/in/_Hot_Temperature?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25158478" data-work_id="25158478" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25158478/Anti_GD1a_ganglioside_antibodies_in_peripheral_motor_syndromes">Anti-GD1a ganglioside antibodies in peripheral motor syndromes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">High titers of anti-GD,, antibodies have been found in patients with Guillain-Bar& syndrome or motor neuropathy. To determine the possible diagnostic relevance of these antibodies, we measured serum anti-GD,, IgG and IgM antibodies by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_25158478" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">High titers of anti-GD,, antibodies have been found in patients with Guillain-Bar& syndrome or motor neuropathy. To determine the possible diagnostic relevance of these antibodies, we measured serum anti-GD,, IgG and IgM antibodies by enzyme-linked immunosorbent assay in 195 patients with different motor syndromes and in 335 control subjects. Moderately high antibody titers (I/ 1,280-1/5,120) were occasionally found in patients with chronic inflammatory demyelinating polyneuropathy (5%), multifocal motor neuropathy (18%), lower motor neuron disease (3.8%), or amyotrophic lateral sclerosis (1.8%) and in immunological control subjects (1.2%), while titers of 1/20,480 or higher were only found in 2 patients with Guillain-Barri! syndrome (IgG in both) and 2 with motor neuropathy and IgMX monoclonal gammopathy improving with immunotherapy. In both motor neuropathy patients and the Guillain-Bar& syndrome patient who were retested during recovery, anti-GD,, titers decreased concomitantly with clinical improvement. High anti-GD,, antibody titers may be found in several motor syndromes but only markedly increased anti-GD,, titers are strictly associated with potentially treatable dysimmune neuropathies. Carpo M, Nobile-Orazio E, Meucci N, Camba M, Barbieri S, Allaria S, Scarlato G. Anti-GD,, ganglioside antibodies in peripheral motor syndromes. Ann Neurol 1996;33:539-543 High titers of serum antibodies to the ganglioside GD,, were demonstrated recently in some patients with Guillain-Bark syndrome (GBS) [l-51 and in a patient with motor neuropathy (MN) associated with IgM monoclonal gammopathy [6], whereas lower antibody levels were detected in patients with motor neuron disease (MND) [7, 81. The anti-GD,, antibodies were mostly IgG in patients with GBS and IgM in those with MN or M N D and the titers, at least in GBS patients [l, 21, decreased concomitantly with clinical improve-From the</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25158478" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="59b85b578bfa9ffc8aa7ab4ade57030f" rel="nofollow" data-download="{"attachment_id":45476664,"asset_id":25158478,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45476664/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="48450310" href="https://unimi.academia.edu/SergioBarbieri">Sergio Barbieri</a><script data-card-contents-for-user="48450310" type="text/json">{"id":48450310,"first_name":"Sergio","last_name":"Barbieri","domain_name":"unimi","page_name":"SergioBarbieri","display_name":"Sergio Barbieri","profile_url":"https://unimi.academia.edu/SergioBarbieri?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_25158478 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25158478"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25158478, container: ".js-paper-rank-work_25158478", }); 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$(".js-view-count[data-work-id=25158478]").text(description); $(".js-view-count-work_25158478").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_25158478").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="25158478"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="51789" href="https://www.academia.edu/Documents/in/Antibodies">Antibodies</a>, <script data-card-contents-for-ri="51789" type="text/json">{"id":51789,"name":"Antibodies","url":"https://www.academia.edu/Documents/in/Antibodies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="244814" href="https://www.academia.edu/Documents/in/Clinical_Sciences">Clinical Sciences</a>, <script data-card-contents-for-ri="244814" type="text/json">{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="289271" href="https://www.academia.edu/Documents/in/Aged">Aged</a>, <script data-card-contents-for-ri="289271" type="text/json">{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="527580" href="https://www.academia.edu/Documents/in/Neuromuscular_diseases">Neuromuscular diseases</a><script data-card-contents-for-ri="527580" type="text/json">{"id":527580,"name":"Neuromuscular diseases","url":"https://www.academia.edu/Documents/in/Neuromuscular_diseases?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=25158478]'), work: {"id":25158478,"title":"Anti-GD1a ganglioside antibodies in peripheral motor syndromes","created_at":"2016-05-09T05:22:30.315-07:00","url":"https://www.academia.edu/25158478/Anti_GD1a_ganglioside_antibodies_in_peripheral_motor_syndromes?f_ri=1716403","dom_id":"work_25158478","summary":"High titers of anti-GD,, antibodies have been found in patients with Guillain-Bar\u0026 syndrome or motor neuropathy. To determine the possible diagnostic relevance of these antibodies, we measured serum anti-GD,, IgG and IgM antibodies by enzyme-linked immunosorbent assay in 195 patients with different motor syndromes and in 335 control subjects. Moderately high antibody titers (I/ 1,280-1/5,120) were occasionally found in patients with chronic inflammatory demyelinating polyneuropathy (5%), multifocal motor neuropathy (18%), lower motor neuron disease (3.8%), or amyotrophic lateral sclerosis (1.8%) and in immunological control subjects (1.2%), while titers of 1/20,480 or higher were only found in 2 patients with Guillain-Barri! syndrome (IgG in both) and 2 with motor neuropathy and IgMX monoclonal gammopathy improving with immunotherapy. In both motor neuropathy patients and the Guillain-Bar\u0026 syndrome patient who were retested during recovery, anti-GD,, titers decreased concomitantly with clinical improvement. High anti-GD,, antibody titers may be found in several motor syndromes but only markedly increased anti-GD,, titers are strictly associated with potentially treatable dysimmune neuropathies. Carpo M, Nobile-Orazio E, Meucci N, Camba M, Barbieri S, Allaria S, Scarlato G. Anti-GD,, ganglioside antibodies in peripheral motor syndromes. Ann Neurol 1996;33:539-543 High titers of serum antibodies to the ganglioside GD,, were demonstrated recently in some patients with Guillain-Bark syndrome (GBS) [l-51 and in a patient with motor neuropathy (MN) associated with IgM monoclonal gammopathy [6], whereas lower antibody levels were detected in patients with motor neuron disease (MND) [7, 81. The anti-GD,, antibodies were mostly IgG in patients with GBS and IgM in those with MN or M N D and the titers, at least in GBS patients [l, 21, decreased concomitantly with clinical improve-From the","downloadable_attachments":[{"id":45476664,"asset_id":25158478,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":48450310,"first_name":"Sergio","last_name":"Barbieri","domain_name":"unimi","page_name":"SergioBarbieri","display_name":"Sergio Barbieri","profile_url":"https://unimi.academia.edu/SergioBarbieri?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":51789,"name":"Antibodies","url":"https://www.academia.edu/Documents/in/Antibodies?f_ri=1716403","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403","nofollow":false},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403","nofollow":false},{"id":527580,"name":"Neuromuscular diseases","url":"https://www.academia.edu/Documents/in/Neuromuscular_diseases?f_ri=1716403","nofollow":false},{"id":710565,"name":"Annals","url":"https://www.academia.edu/Documents/in/Annals?f_ri=1716403"},{"id":888739,"name":"Quntitative Thin Layer Chromatography","url":"https://www.academia.edu/Documents/in/Quntitative_Thin_Layer_Chromatography?f_ri=1716403"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=1716403"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=1716403"},{"id":1334442,"name":"Gangliosides","url":"https://www.academia.edu/Documents/in/Gangliosides?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15534296" data-work_id="15534296" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15534296/Detection_of_immunoglobulin_G_to_measles_virus_rubella_virus_and_mumps_virus_in_serum_samples_and_in_microquantities_of_whole_blood_dried_on_filter_paper">Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Immunity to measles virus, rubella virus, and mumps virus was determined by EIA in serum samples and in dried whole blood specimens spotted on Whatman filter paper (5 mm in diameter). Both specimens were obtained from each patient by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15534296" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Immunity to measles virus, rubella virus, and mumps virus was determined by EIA in serum samples and in dried whole blood specimens spotted on Whatman filter paper (5 mm in diameter). Both specimens were obtained from each patient by venepuncture and finger prick. Ten microliters of whole blood is enough to detect antibodies to these three different viruses. The comparison of the results obtained by EIA from 227 serum and whole blood samples have demonstrated close agreement: 98.6% for measles virus, 99. I o/o for rubella virus, and 96.0%</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15534296" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ed08e60473c3ff4cd43259a376ac2075" rel="nofollow" data-download="{"attachment_id":43114270,"asset_id":15534296,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43114270/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34689799" href="https://independent.academia.edu/AStivala">A. 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In the first... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21476720" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A multicenter effort was begun in 1994 to characterize the pathophysiology of dengue using a study design that minimized patient selection bias by offering enrollment to all children with undifferentiated fever for õ72 h. In the first year, 189 children were enrolled (age range, 8 months to 14 years). Thirty-two percent of these children had dengue infections (60 volunteers). The percentage of children with a secondary dengue infection was 93%, with only 4 (7%) having a primary dengue infection. The virus isolation rate from the plasma of children with dengue was 98%. Viremia correlated highly with temperature. All four dengue virus serotypes were isolated at both study sites. This study demonstrates that all four serotypes of dengue virus can cause dengue hemorrhagic fever, that all dengue patients as defined by serology experience viremia during the febrile phase, and that as fever subsides, so does viremia.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21476720" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="92a6b380afd4fd242c07e9acc3acf169" rel="nofollow" data-download="{"attachment_id":41900623,"asset_id":21476720,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41900623/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42555915" href="https://independent.academia.edu/FrancisEnnis">Francis Ennis</a><script data-card-contents-for-user="42555915" type="text/json">{"id":42555915,"first_name":"Francis","last_name":"Ennis","domain_name":"independent","page_name":"FrancisEnnis","display_name":"Francis Ennis","profile_url":"https://independent.academia.edu/FrancisEnnis?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21476720 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21476720"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21476720, container: ".js-paper-rank-work_21476720", }); 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This study demonstrates that all four serotypes of dengue virus can cause dengue hemorrhagic fever, that all dengue patients as defined by serology experience viremia during the febrile phase, and that as fever subsides, so does viremia.","downloadable_attachments":[{"id":41900623,"asset_id":21476720,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42555915,"first_name":"Francis","last_name":"Ennis","domain_name":"independent","page_name":"FrancisEnnis","display_name":"Francis Ennis","profile_url":"https://independent.academia.edu/FrancisEnnis?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":10096,"name":"Thailand","url":"https://www.academia.edu/Documents/in/Thailand?f_ri=1716403","nofollow":false},{"id":17960,"name":"Infectious 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Nishida</a><script data-card-contents-for-user="42329335" type="text/json">{"id":42329335,"first_name":"Seigo","last_name":"Nishida","domain_name":"independent","page_name":"SeigoNishida","display_name":"Seigo Nishida","profile_url":"https://independent.academia.edu/SeigoNishida?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21140800 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21140800"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21140800, container: ".js-paper-rank-work_21140800", }); });</script></li><li class="js-percentile-work_21140800 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x 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}); });</script></span><script>$(function() { $(".js-view-count-work_21140800").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21140800"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="10610" href="https://www.academia.edu/Documents/in/Survival_Analysis">Survival Analysis</a>, <script data-card-contents-for-ri="10610" type="text/json">{"id":10610,"name":"Survival Analysis","url":"https://www.academia.edu/Documents/in/Survival_Analysis?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12426" href="https://www.academia.edu/Documents/in/Treatment_Outcome">Treatment Outcome</a>, <script data-card-contents-for-ri="12426" type="text/json">{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="108409" href="https://www.academia.edu/Documents/in/Organ_Transplantation">Organ Transplantation</a>, <script data-card-contents-for-ri="108409" type="text/json">{"id":108409,"name":"Organ Transplantation","url":"https://www.academia.edu/Documents/in/Organ_Transplantation?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="134346" href="https://www.academia.edu/Documents/in/Infant">Infant</a><script data-card-contents-for-ri="134346" type="text/json">{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21140800]'), work: {"id":21140800,"title":"Intestinal transplantation in children: a summary of clinical outcomes and prognostic factors in 108 patients from a single center","created_at":"2016-01-29T07:08:31.105-08:00","url":"https://www.academia.edu/21140800/Intestinal_transplantation_in_children_a_summary_of_clinical_outcomes_and_prognostic_factors_in_108_patients_from_a_single_center?f_ri=1716403","dom_id":"work_21140800","summary":null,"downloadable_attachments":[{"id":41733162,"asset_id":21140800,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42329335,"first_name":"Seigo","last_name":"Nishida","domain_name":"independent","page_name":"SeigoNishida","display_name":"Seigo Nishida","profile_url":"https://independent.academia.edu/SeigoNishida?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":10610,"name":"Survival Analysis","url":"https://www.academia.edu/Documents/in/Survival_Analysis?f_ri=1716403","nofollow":false},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1716403","nofollow":false},{"id":108409,"name":"Organ Transplantation","url":"https://www.academia.edu/Documents/in/Organ_Transplantation?f_ri=1716403","nofollow":false},{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=1716403","nofollow":false},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403"},{"id":155901,"name":"Liver Transplantation","url":"https://www.academia.edu/Documents/in/Liver_Transplantation?f_ri=1716403"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1716403"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines?f_ri=1716403"},{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=1716403"},{"id":487159,"name":"Group","url":"https://www.academia.edu/Documents/in/Group?f_ri=1716403"},{"id":489727,"name":"Prognosis","url":"https://www.academia.edu/Documents/in/Prognosis?f_ri=1716403"},{"id":708740,"name":"Hazard Analysis","url":"https://www.academia.edu/Documents/in/Hazard_Analysis?f_ri=1716403"},{"id":893785,"name":"Graft Rejection","url":"https://www.academia.edu/Documents/in/Graft_Rejection?f_ri=1716403"},{"id":922329,"name":"Hazard Rate","url":"https://www.academia.edu/Documents/in/Hazard_Rate?f_ri=1716403"},{"id":972948,"name":"Stepwise Regression","url":"https://www.academia.edu/Documents/in/Stepwise_Regression?f_ri=1716403"},{"id":1198588,"name":"Cyclophosphamide","url":"https://www.academia.edu/Documents/in/Cyclophosphamide?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_18425350" data-work_id="18425350" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/18425350/Cyclosporine_Sparing_with_Mycophenolate_Mofetil_Daclizumab_and_Corticosteroids_in_Renal_Allograft_Recipients_The_CAESAR_Study">Cyclosporine Sparing with Mycophenolate Mofetil, Daclizumab and Corticosteroids in Renal Allograft Recipients: The CAESAR Study</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Although the calcineurin inhibitors (CNI) cyclosporine (CsA) and tacrolimus are highly effective immunosuppressants, they are associated with serious side effects. There is great interest in immunosuppressive regimens that permit... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18425350" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Although the calcineurin inhibitors (CNI) cyclosporine (CsA) and tacrolimus are highly effective immunosuppressants, they are associated with serious side effects. There is great interest in immunosuppressive regimens that permit reduction or elimination of CNIs, while maintaining adequate immunosuppression and acceptable acute rejection rates. Patients (n = 536) receiving their first renal allograft were randomized to one of three immunosuppressant regimens: daclizumab, mycophenolate mofetil (MMF), corticosteroids (CS) and low-dose CsA (target trough levels of 50-100 ng/mL), weaned from month 4 and withdrawn by month 6; daclizumab, MMF, CS and low-dose CsA; or MMF, CS and standard-dose CsA. Mean GFR 12 months after transplantation (primary end point) was not statistically different in the CsA withdrawal and low-dose CsA groups (both 50.9 mL/min/1.73 m(2)) vs. the standard-dose CsA group (48.6 mL/min/1.73 m(2)). At 12 months, the incidence of biopsy-proven acute rejection was significantly higher in the CsA withdrawal group (38%) vs. the low- or standard-dose CsA groups (25.4% and 27.5%, respectively; p &amp;lt; 0.05). In summary, a regimen of continuous low-dose CsA with MMF, CS and daclizumab induction is a clinically safe and effective immunosuppressive regimen in renal transplant recipients.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18425350" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="809a7aa14a3fad20d25fc807639b5777" rel="nofollow" data-download="{"attachment_id":42170176,"asset_id":18425350,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42170176/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38431552" href="https://independent.academia.edu/JosepGrinyo">Josep Grinyo</a><script data-card-contents-for-user="38431552" type="text/json">{"id":38431552,"first_name":"Josep","last_name":"Grinyo","domain_name":"independent","page_name":"JosepGrinyo","display_name":"Josep Grinyo","profile_url":"https://independent.academia.edu/JosepGrinyo?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_18425350 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="18425350"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 18425350, container: ".js-paper-rank-work_18425350", }); });</script></li><li class="js-percentile-work_18425350 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18425350; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_18425350"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_18425350 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="18425350"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18425350; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18425350]").text(description); $(".js-view-count-work_18425350").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_18425350").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="18425350"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="9003" href="https://www.academia.edu/Documents/in/Kidney_transplantation">Kidney transplantation</a>, <script data-card-contents-for-ri="9003" type="text/json">{"id":9003,"name":"Kidney transplantation","url":"https://www.academia.edu/Documents/in/Kidney_transplantation?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12426" href="https://www.academia.edu/Documents/in/Treatment_Outcome">Treatment Outcome</a>, <script data-card-contents-for-ri="12426" type="text/json">{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62112" href="https://www.academia.edu/Documents/in/Prospective_studies">Prospective studies</a>, <script data-card-contents-for-ri="62112" type="text/json">{"id":62112,"name":"Prospective studies","url":"https://www.academia.edu/Documents/in/Prospective_studies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="118540" href="https://www.academia.edu/Documents/in/Immunosuppression">Immunosuppression</a><script data-card-contents-for-ri="118540" type="text/json">{"id":118540,"name":"Immunosuppression","url":"https://www.academia.edu/Documents/in/Immunosuppression?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=18425350]'), work: {"id":18425350,"title":"Cyclosporine Sparing with Mycophenolate Mofetil, Daclizumab and Corticosteroids in Renal Allograft Recipients: The CAESAR Study","created_at":"2015-11-16T02:02:31.173-08:00","url":"https://www.academia.edu/18425350/Cyclosporine_Sparing_with_Mycophenolate_Mofetil_Daclizumab_and_Corticosteroids_in_Renal_Allograft_Recipients_The_CAESAR_Study?f_ri=1716403","dom_id":"work_18425350","summary":"Although the calcineurin inhibitors (CNI) cyclosporine (CsA) and tacrolimus are highly effective immunosuppressants, they are associated with serious side effects. There is great interest in immunosuppressive regimens that permit reduction or elimination of CNIs, while maintaining adequate immunosuppression and acceptable acute rejection rates. Patients (n = 536) receiving their first renal allograft were randomized to one of three immunosuppressant regimens: daclizumab, mycophenolate mofetil (MMF), corticosteroids (CS) and low-dose CsA (target trough levels of 50-100 ng/mL), weaned from month 4 and withdrawn by month 6; daclizumab, MMF, CS and low-dose CsA; or MMF, CS and standard-dose CsA. Mean GFR 12 months after transplantation (primary end point) was not statistically different in the CsA withdrawal and low-dose CsA groups (both 50.9 mL/min/1.73 m(2)) vs. the standard-dose CsA group (48.6 mL/min/1.73 m(2)). At 12 months, the incidence of biopsy-proven acute rejection was significantly higher in the CsA withdrawal group (38%) vs. the low- or standard-dose CsA groups (25.4% and 27.5%, respectively; p \u0026amp;lt; 0.05). In summary, a regimen of continuous low-dose CsA with MMF, CS and daclizumab induction is a clinically safe and effective immunosuppressive regimen in renal transplant recipients.","downloadable_attachments":[{"id":42170176,"asset_id":18425350,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38431552,"first_name":"Josep","last_name":"Grinyo","domain_name":"independent","page_name":"JosepGrinyo","display_name":"Josep Grinyo","profile_url":"https://independent.academia.edu/JosepGrinyo?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9003,"name":"Kidney transplantation","url":"https://www.academia.edu/Documents/in/Kidney_transplantation?f_ri=1716403","nofollow":false},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1716403","nofollow":false},{"id":62112,"name":"Prospective studies","url":"https://www.academia.edu/Documents/in/Prospective_studies?f_ri=1716403","nofollow":false},{"id":118540,"name":"Immunosuppression","url":"https://www.academia.edu/Documents/in/Immunosuppression?f_ri=1716403","nofollow":false},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403"},{"id":151448,"name":"American","url":"https://www.academia.edu/Documents/in/American?f_ri=1716403"},{"id":168352,"name":"Cyclosporine","url":"https://www.academia.edu/Documents/in/Cyclosporine?f_ri=1716403"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403"},{"id":572282,"name":"Combination drug therapy","url":"https://www.academia.edu/Documents/in/Combination_drug_therapy?f_ri=1716403"},{"id":893785,"name":"Graft Rejection","url":"https://www.academia.edu/Documents/in/Graft_Rejection?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1740530,"name":"Mycophenolate mofetil","url":"https://www.academia.edu/Documents/in/Mycophenolate_mofetil?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_32907587" data-work_id="32907587" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/32907587/Protein_binding_properties_of_surface_modified_porous_polyethylene_membranes">Protein binding properties of surface-modified porous polyethylene membranes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In this study, we quantified the adsorption of immunoglobulin G (IgG) protein onto several polyelectrolyte-modified sintered porous polyethylene (PPE) membranes. The polymer surfaces had both cationic and anionic charges obtained via the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_32907587" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In this study, we quantified the adsorption of immunoglobulin G (IgG) protein onto several polyelectrolyte-modified sintered porous polyethylene (PPE) membranes. The polymer surfaces had both cationic and anionic charges obtained via the adsorption of polyethylenimine (PEI) and polyacrylic acid (PAA), respectively, onto plasma-activated PPE. The amount of IgG adsorption was determined by measuring the gamma radiation emitted by [ 125 I]-IgG radio labeled protein. By studying the impact of pH and ionic strength on IgG adsorption, we attempted to characterize the role and nature of the electrostatic interactions involved in the adsorption process to better understand how these interactions were influenced by the charge and structure of immobilized polyelectrolyte complexes at modified membrane surfaces. We were able to show that surface modification of PPE membranes with adsorbed PEI monolayers and PEI-PAA bilayers can greatly improve the IgG binding ability of the membrane under optimized conditions. We also showed that the observed improvement in the IgG binding is derived from electrostatic interactions between IgG and the polyelectrolyte surface. In addition, we found that the greatest IgG adsorption occurred when the IgG and the surface possessed predominantly opposite charges, rather than when the surface possessed the greatest electrostatic charge. Finally, we have found that the molecular weight of the terminating polyelectrolyte has a noticeable effect upon the electrostatic interactions between IgG and the PEI-PAA bilayer-modified PPE surfaces. r</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32907587" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="eb1f752e084b5edef3b0311f739a857c" rel="nofollow" data-download="{"attachment_id":53045015,"asset_id":32907587,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/53045015/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="63967033" href="https://sbsuny.academia.edu/RinaTannenbaum">Rina Tannenbaum</a><script data-card-contents-for-user="63967033" type="text/json">{"id":63967033,"first_name":"Rina","last_name":"Tannenbaum","domain_name":"sbsuny","page_name":"RinaTannenbaum","display_name":"Rina Tannenbaum","profile_url":"https://sbsuny.academia.edu/RinaTannenbaum?f_ri=1716403","photo":"https://0.academia-photos.com/63967033/22811530/21969287/s65_rina.tannenbaum.jpg"}</script></span></span></li><li class="js-paper-rank-work_32907587 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="32907587"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 32907587, container: ".js-paper-rank-work_32907587", }); 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$(".js-view-count[data-work-id=32907587]").text(description); $(".js-view-count-work_32907587").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_32907587").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="32907587"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">31</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2698" href="https://www.academia.edu/Documents/in/Biomaterials">Biomaterials</a>, <script data-card-contents-for-ri="2698" type="text/json">{"id":2698,"name":"Biomaterials","url":"https://www.academia.edu/Documents/in/Biomaterials?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2701" href="https://www.academia.edu/Documents/in/Protein_adsorption">Protein adsorption</a>, <script data-card-contents-for-ri="2701" type="text/json">{"id":2701,"name":"Protein adsorption","url":"https://www.academia.edu/Documents/in/Protein_adsorption?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4319" href="https://www.academia.edu/Documents/in/Polyelectrolytes">Polyelectrolytes</a>, <script data-card-contents-for-ri="4319" type="text/json">{"id":4319,"name":"Polyelectrolytes","url":"https://www.academia.edu/Documents/in/Polyelectrolytes?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="21466" href="https://www.academia.edu/Documents/in/Polymers">Polymers</a><script data-card-contents-for-ri="21466" type="text/json">{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32907587]'), work: {"id":32907587,"title":"Protein binding properties of surface-modified porous polyethylene membranes","created_at":"2017-05-08T08:52:21.464-07:00","url":"https://www.academia.edu/32907587/Protein_binding_properties_of_surface_modified_porous_polyethylene_membranes?f_ri=1716403","dom_id":"work_32907587","summary":"In this study, we quantified the adsorption of immunoglobulin G (IgG) protein onto several polyelectrolyte-modified sintered porous polyethylene (PPE) membranes. The polymer surfaces had both cationic and anionic charges obtained via the adsorption of polyethylenimine (PEI) and polyacrylic acid (PAA), respectively, onto plasma-activated PPE. The amount of IgG adsorption was determined by measuring the gamma radiation emitted by [ 125 I]-IgG radio labeled protein. By studying the impact of pH and ionic strength on IgG adsorption, we attempted to characterize the role and nature of the electrostatic interactions involved in the adsorption process to better understand how these interactions were influenced by the charge and structure of immobilized polyelectrolyte complexes at modified membrane surfaces. We were able to show that surface modification of PPE membranes with adsorbed PEI monolayers and PEI-PAA bilayers can greatly improve the IgG binding ability of the membrane under optimized conditions. We also showed that the observed improvement in the IgG binding is derived from electrostatic interactions between IgG and the polyelectrolyte surface. In addition, we found that the greatest IgG adsorption occurred when the IgG and the surface possessed predominantly opposite charges, rather than when the surface possessed the greatest electrostatic charge. Finally, we have found that the molecular weight of the terminating polyelectrolyte has a noticeable effect upon the electrostatic interactions between IgG and the PEI-PAA bilayer-modified PPE surfaces. r","downloadable_attachments":[{"id":53045015,"asset_id":32907587,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":63967033,"first_name":"Rina","last_name":"Tannenbaum","domain_name":"sbsuny","page_name":"RinaTannenbaum","display_name":"Rina Tannenbaum","profile_url":"https://sbsuny.academia.edu/RinaTannenbaum?f_ri=1716403","photo":"https://0.academia-photos.com/63967033/22811530/21969287/s65_rina.tannenbaum.jpg"}],"research_interests":[{"id":2698,"name":"Biomaterials","url":"https://www.academia.edu/Documents/in/Biomaterials?f_ri=1716403","nofollow":false},{"id":2701,"name":"Protein adsorption","url":"https://www.academia.edu/Documents/in/Protein_adsorption?f_ri=1716403","nofollow":false},{"id":4319,"name":"Polyelectrolytes","url":"https://www.academia.edu/Documents/in/Polyelectrolytes?f_ri=1716403","nofollow":false},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers?f_ri=1716403","nofollow":false},{"id":22998,"name":"Gamma Rays","url":"https://www.academia.edu/Documents/in/Gamma_Rays?f_ri=1716403"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1716403"},{"id":39752,"name":"Adsorption","url":"https://www.academia.edu/Documents/in/Adsorption?f_ri=1716403"},{"id":79275,"name":"Surface modification","url":"https://www.academia.edu/Documents/in/Surface_modification?f_ri=1716403"},{"id":168694,"name":"Polyethylene","url":"https://www.academia.edu/Documents/in/Polyethylene?f_ri=1716403"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=1716403"},{"id":263252,"name":"Anions","url":"https://www.academia.edu/Documents/in/Anions?f_ri=1716403"},{"id":373249,"name":"Electrolytes","url":"https://www.academia.edu/Documents/in/Electrolytes?f_ri=1716403"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=1716403"},{"id":604754,"name":"Acrylic Resins","url":"https://www.academia.edu/Documents/in/Acrylic_Resins?f_ri=1716403"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight?f_ri=1716403"},{"id":758900,"name":"Lipid bilayers","url":"https://www.academia.edu/Documents/in/Lipid_bilayers?f_ri=1716403"},{"id":892969,"name":"Materials Testing","url":"https://www.academia.edu/Documents/in/Materials_Testing?f_ri=1716403"},{"id":907131,"name":"Polyethylenes","url":"https://www.academia.edu/Documents/in/Polyethylenes?f_ri=1716403"},{"id":970774,"name":"Ionic Strength","url":"https://www.academia.edu/Documents/in/Ionic_Strength?f_ri=1716403"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding?f_ri=1716403"},{"id":1013858,"name":"Cations","url":"https://www.academia.edu/Documents/in/Cations?f_ri=1716403"},{"id":1031067,"name":"Biocompatible Materials","url":"https://www.academia.edu/Documents/in/Biocompatible_Materials?f_ri=1716403"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=1716403"},{"id":1180667,"name":"Optimality Condition","url":"https://www.academia.edu/Documents/in/Optimality_Condition?f_ri=1716403"},{"id":1257974,"name":"Ions","url":"https://www.academia.edu/Documents/in/Ions?f_ri=1716403"},{"id":1266470,"name":"Polyethylenimine","url":"https://www.academia.edu/Documents/in/Polyethylenimine?f_ri=1716403"},{"id":1426712,"name":"Immunoglobulin","url":"https://www.academia.edu/Documents/in/Immunoglobulin?f_ri=1716403"},{"id":1438957,"name":"Polyethyleneimine","url":"https://www.academia.edu/Documents/in/Polyethyleneimine?f_ri=1716403"},{"id":1451660,"name":"Static Electricity","url":"https://www.academia.edu/Documents/in/Static_Electricity?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1997650,"name":"Gamma radiation","url":"https://www.academia.edu/Documents/in/Gamma_radiation?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_69342757" data-work_id="69342757" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/69342757/C_reactive_protein_mediates_the_effect_of_apolipoprotein_E_on_cytomegalovirus_infection">C-reactive protein mediates the effect of apolipoprotein E on cytomegalovirus infection</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background. Although the apolipoprotein (APOE)-4 allele has been shown to determine the outcome of several infections, its relationship with cytomegalovirus (CMV) has not been explored. We examine whether APOE determines CMV and herpes... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_69342757" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background. Although the apolipoprotein (APOE)-4 allele has been shown to determine the outcome of several infections, its relationship with cytomegalovirus (CMV) has not been explored. We examine whether APOE determines CMV and herpes simplex virus type 1 (HSV-1) antibody levels and assess whether C-reactive protein (CRP) mediates any observed relationships. Methods. We conducted a cross-sectional analysis of a randomly selected subset (n ϭ 1561/1789) of participants aged 60-101 in the Sacramento Area Latino Study on Aging. Blood samples were tested for APOE genotype, CRP, and immunoglobulin G antibodies to CMV and HSV-1. Multivariate logistic regression was used to examine the association between 4 and CMV and HSV antibody levels. We also assessed whether CRP mediates the effects of any observed associations between 4 and viral antibody levels. Results. CMV antibody and CRP levels varied significantly by APOE genotype. The association between CRP and CMV antibody was strengthened in the presence of 4. In contrast, this effect was not observed in HSV-1. We found that APOE-4 carriers had significantly lower levels of CRP yet significantly higher levels of CMV antibodies, suggesting a mediating pathway. Conclusions. APOE-4 carriers may experience immunological aberrations that lead to lower levels of CRP and correspondingly higher CMV antibody levels.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/69342757" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="eaf8acc8112cd4ad2d47537317d16a2c" rel="nofollow" data-download="{"attachment_id":79475953,"asset_id":69342757,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/79475953/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="58058515" href="https://independent.academia.edu/HoangoanhNguyen4">Hoangoanh Nguyen</a><script data-card-contents-for-user="58058515" type="text/json">{"id":58058515,"first_name":"Hoangoanh","last_name":"Nguyen","domain_name":"independent","page_name":"HoangoanhNguyen4","display_name":"Hoangoanh Nguyen","profile_url":"https://independent.academia.edu/HoangoanhNguyen4?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_69342757 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="69342757"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 69342757, container: ".js-paper-rank-work_69342757", }); });</script></li><li class="js-percentile-work_69342757 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 69342757; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_69342757"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_69342757 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="69342757"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 69342757; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=69342757]").text(description); $(".js-view-count-work_69342757").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_69342757").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="69342757"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="586" href="https://www.academia.edu/Documents/in/Health_Sciences">Health Sciences</a>, <script data-card-contents-for-ri="586" type="text/json">{"id":586,"name":"Health Sciences","url":"https://www.academia.edu/Documents/in/Health_Sciences?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16288" href="https://www.academia.edu/Documents/in/Public_Health">Public Health</a>, <script data-card-contents-for-ri="16288" type="text/json">{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="32433" href="https://www.academia.edu/Documents/in/Logistic_Regression">Logistic Regression</a>, <script data-card-contents-for-ri="32433" type="text/json">{"id":32433,"name":"Logistic Regression","url":"https://www.academia.edu/Documents/in/Logistic_Regression?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=69342757]'), work: {"id":69342757,"title":"C-reactive protein mediates the effect of apolipoprotein E on cytomegalovirus infection","created_at":"2022-01-24T03:54:57.323-08:00","url":"https://www.academia.edu/69342757/C_reactive_protein_mediates_the_effect_of_apolipoprotein_E_on_cytomegalovirus_infection?f_ri=1716403","dom_id":"work_69342757","summary":"Background. Although the apolipoprotein (APOE)-4 allele has been shown to determine the outcome of several infections, its relationship with cytomegalovirus (CMV) has not been explored. We examine whether APOE determines CMV and herpes simplex virus type 1 (HSV-1) antibody levels and assess whether C-reactive protein (CRP) mediates any observed relationships. Methods. We conducted a cross-sectional analysis of a randomly selected subset (n ϭ 1561/1789) of participants aged 60-101 in the Sacramento Area Latino Study on Aging. Blood samples were tested for APOE genotype, CRP, and immunoglobulin G antibodies to CMV and HSV-1. Multivariate logistic regression was used to examine the association between 4 and CMV and HSV antibody levels. We also assessed whether CRP mediates the effects of any observed associations between 4 and viral antibody levels. Results. CMV antibody and CRP levels varied significantly by APOE genotype. The association between CRP and CMV antibody was strengthened in the presence of 4. In contrast, this effect was not observed in HSV-1. We found that APOE-4 carriers had significantly lower levels of CRP yet significantly higher levels of CMV antibodies, suggesting a mediating pathway. Conclusions. APOE-4 carriers may experience immunological aberrations that lead to lower levels of CRP and correspondingly higher CMV antibody levels.","downloadable_attachments":[{"id":79475953,"asset_id":69342757,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":58058515,"first_name":"Hoangoanh","last_name":"Nguyen","domain_name":"independent","page_name":"HoangoanhNguyen4","display_name":"Hoangoanh Nguyen","profile_url":"https://independent.academia.edu/HoangoanhNguyen4?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":586,"name":"Health Sciences","url":"https://www.academia.edu/Documents/in/Health_Sciences?f_ri=1716403","nofollow":false},{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health?f_ri=1716403","nofollow":false},{"id":32433,"name":"Logistic Regression","url":"https://www.academia.edu/Documents/in/Logistic_Regression?f_ri=1716403","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1716403","nofollow":false},{"id":49753,"name":"Herpes Simplex","url":"https://www.academia.edu/Documents/in/Herpes_Simplex?f_ri=1716403"},{"id":82145,"name":"Virus","url":"https://www.academia.edu/Documents/in/Virus?f_ri=1716403"},{"id":187810,"name":"Apolipoprotein E","url":"https://www.academia.edu/Documents/in/Apolipoprotein_E?f_ri=1716403"},{"id":210665,"name":"Infectious","url":"https://www.academia.edu/Documents/in/Infectious?f_ri=1716403"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=1716403"},{"id":295452,"name":"C reactive protein","url":"https://www.academia.edu/Documents/in/C_reactive_protein?f_ri=1716403"},{"id":404000,"name":"Cross Section","url":"https://www.academia.edu/Documents/in/Cross_Section?f_ri=1716403"},{"id":699649,"name":"Cytomegalovirus Infections","url":"https://www.academia.edu/Documents/in/Cytomegalovirus_Infections?f_ri=1716403"},{"id":1034181,"name":"Cross Sectional Studies","url":"https://www.academia.edu/Documents/in/Cross_Sectional_Studies?f_ri=1716403"},{"id":1294607,"name":"Logistic Models","url":"https://www.academia.edu/Documents/in/Logistic_Models?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_63219470" data-work_id="63219470" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/63219470/A_novel_chemistry_for_conjugating_pneumococcal_polysaccharides_to_Luminex_microspheres">A novel chemistry for conjugating pneumococcal polysaccharides to Luminex microspheres</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Here we describe a novel method to conjugate pneumococcal polysaccharides (PnPS) to Luminex microspheres for use in serological assays. 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium (DMTMM) modification of PnPS and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_63219470" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Here we describe a novel method to conjugate pneumococcal polysaccharides (PnPS) to Luminex microspheres for use in serological assays. 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium (DMTMM) modification of PnPS and conjugation to carboxyl functional groups on Luminex microspheres (COOH-DMTMM method) was shown to be a reproducible chemistry that efficiently conjugated PnPS to Luminex microspheres without affecting the antigenicity of a broad set of PnPS. The COOH-DMTMM method was compared to three other methods for robustness, reproducibility and effect on PnPS antigenicity in a multiplexed assay format. The other methods examined included adsorption of the unmodified PnPS to Luminex microspheres, oxidation of the PnPS to conjugate them to amino-modified microspheres using carbodiimide chemistry and poly-l-lysine modification of the PnPS before conjugating to carboxy Luminex microspheres using carbodiimide chemistry. Of the four methods, the COOH-DMTMM chemistry was shown to be a robust methodology, producing stable PnPS coupled microspheres with a 4-log dynamic range and low cross-reactivity when used in a PnPS-specific IgG serology assay. This novel chemistry should be useful for developing serological assays to measure antibodies to polysaccharides for use in vaccine and epidemiology studies.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/63219470" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6e0b1af103bc9dcd627d45da7bda1b3a" rel="nofollow" data-download="{"attachment_id":75717534,"asset_id":63219470,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75717534/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43640867" href="https://independent.academia.edu/MarkEsser">Mark Esser</a><script data-card-contents-for-user="43640867" type="text/json">{"id":43640867,"first_name":"Mark","last_name":"Esser","domain_name":"independent","page_name":"MarkEsser","display_name":"Mark Esser","profile_url":"https://independent.academia.edu/MarkEsser?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_63219470 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="63219470"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 63219470, container: ".js-paper-rank-work_63219470", }); 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$(".js-view-count[data-work-id=63219470]").text(description); $(".js-view-count-work_63219470").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_63219470").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="63219470"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28920" href="https://www.academia.edu/Documents/in/Streptococcus_pneumoniae">Streptococcus pneumoniae</a>, <script data-card-contents-for-ri="28920" type="text/json">{"id":28920,"name":"Streptococcus pneumoniae","url":"https://www.academia.edu/Documents/in/Streptococcus_pneumoniae?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="359001" href="https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology">Optometry and Ophthalmology</a>, <script data-card-contents-for-ri="359001" type="text/json">{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="511535" href="https://www.academia.edu/Documents/in/Immunoassay">Immunoassay</a><script data-card-contents-for-ri="511535" type="text/json">{"id":511535,"name":"Immunoassay","url":"https://www.academia.edu/Documents/in/Immunoassay?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=63219470]'), work: {"id":63219470,"title":"A novel chemistry for conjugating pneumococcal polysaccharides to Luminex microspheres","created_at":"2021-12-04T16:30:30.002-08:00","url":"https://www.academia.edu/63219470/A_novel_chemistry_for_conjugating_pneumococcal_polysaccharides_to_Luminex_microspheres?f_ri=1716403","dom_id":"work_63219470","summary":"Here we describe a novel method to conjugate pneumococcal polysaccharides (PnPS) to Luminex microspheres for use in serological assays. 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium (DMTMM) modification of PnPS and conjugation to carboxyl functional groups on Luminex microspheres (COOH-DMTMM method) was shown to be a reproducible chemistry that efficiently conjugated PnPS to Luminex microspheres without affecting the antigenicity of a broad set of PnPS. The COOH-DMTMM method was compared to three other methods for robustness, reproducibility and effect on PnPS antigenicity in a multiplexed assay format. The other methods examined included adsorption of the unmodified PnPS to Luminex microspheres, oxidation of the PnPS to conjugate them to amino-modified microspheres using carbodiimide chemistry and poly-l-lysine modification of the PnPS before conjugating to carboxy Luminex microspheres using carbodiimide chemistry. Of the four methods, the COOH-DMTMM chemistry was shown to be a robust methodology, producing stable PnPS coupled microspheres with a 4-log dynamic range and low cross-reactivity when used in a PnPS-specific IgG serology assay. This novel chemistry should be useful for developing serological assays to measure antibodies to polysaccharides for use in vaccine and epidemiology studies.","downloadable_attachments":[{"id":75717534,"asset_id":63219470,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":43640867,"first_name":"Mark","last_name":"Esser","domain_name":"independent","page_name":"MarkEsser","display_name":"Mark Esser","profile_url":"https://independent.academia.edu/MarkEsser?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1716403","nofollow":false},{"id":28920,"name":"Streptococcus pneumoniae","url":"https://www.academia.edu/Documents/in/Streptococcus_pneumoniae?f_ri=1716403","nofollow":false},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology?f_ri=1716403","nofollow":false},{"id":511535,"name":"Immunoassay","url":"https://www.academia.edu/Documents/in/Immunoassay?f_ri=1716403","nofollow":false},{"id":519926,"name":"Functional Group","url":"https://www.academia.edu/Documents/in/Functional_Group?f_ri=1716403"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres?f_ri=1716403"},{"id":1557770,"name":"Pneumococcal Vaccines","url":"https://www.academia.edu/Documents/in/Pneumococcal_Vaccines?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1956581,"name":"Dynamic Range","url":"https://www.academia.edu/Documents/in/Dynamic_Range?f_ri=1716403"},{"id":2637271,"name":"Reference standards","url":"https://www.academia.edu/Documents/in/Reference_standards?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_55284200" data-work_id="55284200" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/55284200/Increased_phagocytosis_of_platelets_from_patients_with_secondary_dengue_virus_infection_by_human_macrophages">Increased phagocytosis of platelets from patients with secondary dengue virus infection by human macrophages</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_55284200" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the convalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P = 0.011) and the levels of PAIgG (P = 0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/55284200" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f853566392c3052628503408bfe8e41d" rel="nofollow" data-download="{"attachment_id":71226998,"asset_id":55284200,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/71226998/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="63965461" href="https://independent.academia.edu/ShingoInoue">Shingo Inoue</a><script data-card-contents-for-user="63965461" type="text/json">{"id":63965461,"first_name":"Shingo","last_name":"Inoue","domain_name":"independent","page_name":"ShingoInoue","display_name":"Shingo Inoue","profile_url":"https://independent.academia.edu/ShingoInoue?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_55284200 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="55284200"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 55284200, container: ".js-paper-rank-work_55284200", }); 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$(".js-view-count[data-work-id=55284200]").text(description); $(".js-view-count-work_55284200").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_55284200").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="55284200"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="6599" href="https://www.academia.edu/Documents/in/Flow_Cytometry">Flow Cytometry</a>, <script data-card-contents-for-ri="6599" type="text/json">{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="8241" href="https://www.academia.edu/Documents/in/Tropical_Medicine">Tropical Medicine</a>, <script data-card-contents-for-ri="8241" type="text/json">{"id":8241,"name":"Tropical Medicine","url":"https://www.academia.edu/Documents/in/Tropical_Medicine?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="17491" href="https://www.academia.edu/Documents/in/Macrophages">Macrophages</a>, <script data-card-contents-for-ri="17491" type="text/json">{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a><script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=55284200]'), work: {"id":55284200,"title":"Increased phagocytosis of platelets from patients with secondary dengue virus infection by human macrophages","created_at":"2021-10-03T19:32:40.330-07:00","url":"https://www.academia.edu/55284200/Increased_phagocytosis_of_platelets_from_patients_with_secondary_dengue_virus_infection_by_human_macrophages?f_ri=1716403","dom_id":"work_55284200","summary":"The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the convalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P = 0.011) and the levels of PAIgG (P = 0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in...","downloadable_attachments":[{"id":71226998,"asset_id":55284200,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":63965461,"first_name":"Shingo","last_name":"Inoue","domain_name":"independent","page_name":"ShingoInoue","display_name":"Shingo Inoue","profile_url":"https://independent.academia.edu/ShingoInoue?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=1716403","nofollow":false},{"id":8241,"name":"Tropical Medicine","url":"https://www.academia.edu/Documents/in/Tropical_Medicine?f_ri=1716403","nofollow":false},{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages?f_ri=1716403","nofollow":false},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=1716403","nofollow":false},{"id":52136,"name":"Platelet","url":"https://www.academia.edu/Documents/in/Platelet?f_ri=1716403"},{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=1716403"},{"id":157268,"name":"Dengue","url":"https://www.academia.edu/Documents/in/Dengue?f_ri=1716403"},{"id":376644,"name":"Phagocytosis","url":"https://www.academia.edu/Documents/in/Phagocytosis?f_ri=1716403"},{"id":1281380,"name":"Platelet Count","url":"https://www.academia.edu/Documents/in/Platelet_Count?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":1819399,"name":"Case Control Studies","url":"https://www.academia.edu/Documents/in/Case_Control_Studies?f_ri=1716403"},{"id":1863718,"name":"The American","url":"https://www.academia.edu/Documents/in/The_American?f_ri=1716403"},{"id":3187114,"name":"Blood platelets","url":"https://www.academia.edu/Documents/in/Blood_platelets?f_ri=1716403"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_49383824" data-work_id="49383824" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/49383824/Large_scale_production_and_purification_of_paraquat_and_desipramine_monoclonal_antibodies_and_their_Fab_fragments">Large scale production and purification of paraquat and desipramine monoclonal antibodies and their Fab fragments</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We describe the rapid, large scale purification of Fab fragments from mouse monoclonal antibodies. Antibodies against two clinically important and often fatal toxins, paraquat and desipramine, were isolated from mouse ascites fluid by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_49383824" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We describe the rapid, large scale purification of Fab fragments from mouse monoclonal antibodies. Antibodies against two clinically important and often fatal toxins, paraquat and desipramine, were isolated from mouse ascites fluid by preparative high performance hydroxylapatite (HPHT) or ion exchange (DEAE) high performance liquid chromatography. A competitive inhibition ELISA was used to determine the cross-reactivity of the antibody with analogs of the antigens. Papain digests of the IgGs were subjected to further HPHT followed by Sephadex G-100 chromatography to yield homogeneous Fab fragment preparations. The high purity of these preparations, demonstrated by SDS polyacrylamide gel electrophoresis, has only been achieved previously by affinity chromatography. Intrinsic association constants for the intact IgG and the Fab fragment--antigen interactions, determined by competitive inhibition ELISA, were similar. This indicates that antigen-binding activity was conserved during the...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/49383824" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c308c4df1ead5054d90be2787a72174b" rel="nofollow" data-download="{"attachment_id":67752730,"asset_id":49383824,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67752730/download_file?st=MTczMzkxNjYzMSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="23558036" href="https://independent.academia.edu/MarlinBowles">Marlin Bowles</a><script data-card-contents-for-user="23558036" type="text/json">{"id":23558036,"first_name":"Marlin","last_name":"Bowles","domain_name":"independent","page_name":"MarlinBowles","display_name":"Marlin Bowles","profile_url":"https://independent.academia.edu/MarlinBowles?f_ri=1716403","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_49383824 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="49383824"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 49383824, container: ".js-paper-rank-work_49383824", }); 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$(".js-view-count[data-work-id=49383824]").text(description); $(".js-view-count-work_49383824").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_49383824").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="49383824"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="26699" href="https://www.academia.edu/Documents/in/Immunopharmacology">Immunopharmacology</a>, <script data-card-contents-for-ri="26699" type="text/json">{"id":26699,"name":"Immunopharmacology","url":"https://www.academia.edu/Documents/in/Immunopharmacology?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="147196" href="https://www.academia.edu/Documents/in/Monoclonal_Antibodies">Monoclonal Antibodies</a>, <script data-card-contents-for-ri="147196" type="text/json">{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="213340" href="https://www.academia.edu/Documents/in/Paraquat">Paraquat</a><script data-card-contents-for-ri="213340" type="text/json">{"id":213340,"name":"Paraquat","url":"https://www.academia.edu/Documents/in/Paraquat?f_ri=1716403","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=49383824]'), work: {"id":49383824,"title":"Large scale production and purification of paraquat and desipramine monoclonal antibodies and their Fab fragments","created_at":"2021-06-25T21:53:18.189-07:00","url":"https://www.academia.edu/49383824/Large_scale_production_and_purification_of_paraquat_and_desipramine_monoclonal_antibodies_and_their_Fab_fragments?f_ri=1716403","dom_id":"work_49383824","summary":"We describe the rapid, large scale purification of Fab fragments from mouse monoclonal antibodies. Antibodies against two clinically important and often fatal toxins, paraquat and desipramine, were isolated from mouse ascites fluid by preparative high performance hydroxylapatite (HPHT) or ion exchange (DEAE) high performance liquid chromatography. A competitive inhibition ELISA was used to determine the cross-reactivity of the antibody with analogs of the antigens. Papain digests of the IgGs were subjected to further HPHT followed by Sephadex G-100 chromatography to yield homogeneous Fab fragment preparations. The high purity of these preparations, demonstrated by SDS polyacrylamide gel electrophoresis, has only been achieved previously by affinity chromatography. Intrinsic association constants for the intact IgG and the Fab fragment--antigen interactions, determined by competitive inhibition ELISA, were similar. This indicates that antigen-binding activity was conserved during the...","downloadable_attachments":[{"id":67752730,"asset_id":49383824,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":23558036,"first_name":"Marlin","last_name":"Bowles","domain_name":"independent","page_name":"MarlinBowles","display_name":"Marlin Bowles","profile_url":"https://independent.academia.edu/MarlinBowles?f_ri=1716403","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":26699,"name":"Immunopharmacology","url":"https://www.academia.edu/Documents/in/Immunopharmacology?f_ri=1716403","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1716403","nofollow":false},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=1716403","nofollow":false},{"id":213340,"name":"Paraquat","url":"https://www.academia.edu/Documents/in/Paraquat?f_ri=1716403","nofollow":false},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=1716403"},{"id":758278,"name":"Large Scale","url":"https://www.academia.edu/Documents/in/Large_Scale?f_ri=1716403"},{"id":798093,"name":"Hydroxyapatites","url":"https://www.academia.edu/Documents/in/Hydroxyapatites?f_ri=1716403"},{"id":945593,"name":"Durapatite","url":"https://www.academia.edu/Documents/in/Durapatite?f_ri=1716403"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G?f_ri=1716403"},{"id":2673056,"name":"desipramine","url":"https://www.academia.edu/Documents/in/desipramine?f_ri=1716403"}]}, }) } })();</script></ul></li></ul></div></div></div><div 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