Primer Design for the Detection of Secondary Metabolite Biosynthetic Pathways in Metagenomic Data

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</div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Primer Design for the Detection of Secondary Metabolite Biosynthetic Pathways in Metagenomic Data</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jeisson%20Alejandro%20Triana">Jeisson Alejandro Triana</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Fernanda%20Quiceno%20Vallejo"> Maria Fernanda Quiceno Vallejo</a>, <a href="https://publications.waset.org/abstracts/search?q=Patricia%20del%20Portillo"> Patricia del Portillo</a>, <a href="https://publications.waset.org/abstracts/search?q=Juan%20Manuel%20Anzola"> Juan Manuel Anzola</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Most of the known antimicrobials so far discovered are secondary metabolites. The potential for new natural products of this category increases as new microbial genomes and metagenomes are being sequenced. Despite the advances, there is no systematic way to interrogate metagenomic clones for their potential to contain clusters of genes related to these pathways. Here we analyzed 52 biosynthetic pathways from the AntiSMASH database at the protein domain level in order to identify domains of high specificity and sensitivity with respect to specific biosynthetic pathways. These domains turned out to have various degrees of divergence at the DNA level. We propose PCR assays targetting such domains in-silico and corroborated one by Sanger sequencing. <iframe src="https://publications.waset.org/abstracts/144241.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioinformatic" title="bioinformatic">bioinformatic</a>, <a href="https://publications.waset.org/abstracts/search?q=anti%20smash" title=" anti smash"> anti smash</a>, <a href="https://publications.waset.org/abstracts/search?q=antibiotics" title=" antibiotics"> antibiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=secondary%20metabolites" title=" secondary metabolites"> secondary metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20products" title=" natural products"> natural products</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20domains" title=" protein domains"> protein domains</a> </p> <a href="https://publications.waset.org/abstracts/144241/primer-design-for-the-detection-of-secondary-metabolite-biosynthetic-pathways-in-metagenomic-data" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144241.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">179</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational 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Primer Design for the Detection of Secondary Metabolite Biosynthetic Pathways in Metagenomic Data