HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers</title> <meta name="description" content="HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers"> <meta name="keywords" content="Biomarkers, fingerprint profiling, gallic acid, HPTLC, Protorhus longifolia."> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <meta name="citation_title" content="HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers"> <meta name="citation_author" content="P. S. Seboletswe"> <meta name="citation_author" content="Z. Mkhize"> <meta name="citation_author" content="L. M. Katata-Seru"> <meta name="citation_publication_date" content="2019/11/02"> <meta name="citation_journal_title" content="International Journal of Materials and Metallurgical Engineering"> <meta name="citation_volume" content="13"> <meta name="citation_issue" content="12"> <meta name="citation_firstpage" content="553"> <meta name="citation_lastpage" content="557"> <meta name="citation_pdf_url" content="https://publications.waset.org/10010946/pdf"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value=""> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 33093</div> </div> </div> </div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=P.%20S.%20Seboletswe">P. S. Seboletswe</a>, <a href="https://publications.waset.org/search?q=Z.%20Mkhize"> Z. Mkhize</a>, <a href="https://publications.waset.org/search?q=L.%20M.%20Katata-Seru"> L. M. Katata-Seru</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p><em>Protorhus longifolia </em>is known as a medicinal plant that has been used traditionally to treat various ailments such as hemiplegic paralysis, blood clotting related diseases, diarrhoea, heartburn, etc. The study reports a High-Performance Thin Layer Chromatography (HPTLC) fingerprint profile of <em>Protorhus longifolia</em> methanolic extract and its qualitative analysis of gallic acid, rutin, and quercetin. HPTLC analysis was achieved using CAMAG HPTLC system equipped with CAMAG automatic TLC sampler 4, CAMAG Automatic Developing Chamber 2 (ADC2), CAMAG visualizer 2, CAMAG Thin Layer Chromatography (TLC) scanner and visionCATS CAMAG HPTLC software. Mobile phase comprising toluene, ethyl acetate, formic acid (21:15:3) was used for qualitative analysis of gallic acid and revealed eight peaks while the mobile phase containing ethyl acetate, water, glacial acetic acid, formic acid (100:26:11:11) for qualitative analysis of rutin and quercetin revealed six peaks. HPTLC sillica gel 60 F254 glass plates (10 × 10) were used as the stationary phase. Gallic acid was detected at the R<sub>f</sub> = 0.35; while rutin and quercetin were not evident in the extract. Further studies will be performed to quantify gallic acid in <em>Protorhus longifolia</em> leaves and also identify other biomarkers.</p> <iframe src="https://publications.waset.org/10010946.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Biomarkers" title="Biomarkers">Biomarkers</a>, <a href="https://publications.waset.org/search?q=fingerprint%20profiling" title=" fingerprint profiling"> fingerprint profiling</a>, <a href="https://publications.waset.org/search?q=gallic%20acid" title=" gallic acid"> gallic acid</a>, <a href="https://publications.waset.org/search?q=HPTLC" title=" HPTLC"> HPTLC</a>, <a href="https://publications.waset.org/search?q=Protorhus%20longifolia." title=" Protorhus longifolia."> Protorhus longifolia.</a> </p> <p class="card-text"><strong>Digital Object Identifier (DOI):</strong> <a href="https://doi.org/10.5281/zenodo.3593224" target="_blank">doi.org/10.5281/zenodo.3593224</a> </p> <a href="https://publications.waset.org/10010946/hptlc-fingerprint-profiling-of-protorhus-longifolia-methanolic-leaf-extract-and-qualitative-analysis-of-common-biomarkers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10010946/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10010946/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10010946/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10010946/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10010946/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10010946/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10010946/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10010946/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10010946/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10010946/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10010946.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">844</span> </span> <p class="card-text"><strong>References:</strong></p> <br>[1] R. A. Street and G. Prinsloo, “Commercially important medicinal plants of South Africa”, J. Chem. vol. 2013, pp. 1-16, November 2012. <br>[2] M. Saxena, J. Saxena, R Nema, D. Singh and A. Gupta “Phytochemistry of medicinal plants”, J Pharmacogn Phytochem. vol. 1, pp. 168-182, March 2013. <br>[3] Y. A. Barku, Wound Healing-Current Perspectives. IntechOpen, 2019, ch.5. <br>[4] M. F. Mahomoodally, “Traditional medicines in Africa: An appraisal of ten potent African medicinal plants”, Evid Based Complement Alternat Med. vol. 2013, pp. 1-14, October 2013. <br>[5] S. Van Vuuren and D. Holl, "Antimicrobial natural product research: a review from a South African perspective for the years 2009–2016”, J. Ethnopharmacol. vol. 208, pp. 236-252, August 2017. <br>[6] L. V. Buwa-Komoren, B. Mayekiso, Z. Mhinana, and A. L. Adeniran, “An ethnobotanical and ethnomedicinal survey of traditionally used medicinal plants in Seymour, South Africa: An attempt toward digitization and preservation of ethnic knowledge”, Pharmacogn. Mag. vol. 15, pp. 115-123, January 2019. <br>[7] S. H. Nile and Park, S. W, “HPTLC analysis, antioxidant and antigout activity of Indian plants. Iran J Pharm Res. vol. 13, pp. 531-539, September 2014. <br>[8] L. Seasotiya, P. Siwach, A. Malik, S. Bai, P. Bharti and S. Dalal, “ Phytochemical evaluation and HPTLC fingerprint profile of Cassia fistula”, Int. J. Adv. Pharm., Biol. Chem. vol. 3, pp. 604-611, September 2014. <br>[9] A. K. Chandrakar, “Phytochemical fingerprinting through computerized HPTLC System for quality control of herbal drugs”, nt. j. res. appl. sci. eng. technol. vol. 6, pp. 733-738, January 2019. <br>[10] D. Gomathi, G. Ravikumar, M. Kalaiselvi, B. Vidya and C. Uma, “HPTLC fingerprinting analysis of Evolvulus alsinoides (L.) L.”, J. Acute Med. vol. 3, pp. 77-82, September 2012. <br>[11] D. Penduka, N. P. Gasa, M. S. Hlongwane, R. A. Mosa, F. O. Osunsanmi, and A. R. Opoku, “The antibacterial activities of some plant-derived triterpenes” afr j tradit complem. vol. 12, pp. 180-188, September 2015. <br>[12] R. A. Mosa, Nhleko, M.L., Dladla, T.V. and Opoku, A.R, “Antibacterial activity of two triterpenes from stem bark of Protorhus longifolia”, J. Med. Plant Res. vol. 8, pp. 686-702, May 2014. <br>[13] M. M. Suleiman, L. I. McGaw, V. Naidoo and J. Eloff, “Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species” AFR J TRADIT COMPLEM. vol. 7, pp. 64-78, October 2009. <br>[14] R. A. Mosa, G. G. Lazarus, P. E. Gwala, A. O. Oyedeji, and A. R. Opoku, “In vitro anti-platelet aggregation, antioxidant and cytotoxic activity of extracts of some Zulu medicinal plants”, J Nat Prod. vol. 4, pp. 136-146, June 2011. <br>[15] C. I. Sajeeth, “Quantitative estimation of gallic acid, rutin and quercetin in certain herbal plants by HPTLC method”, Der Chemica Sinica. vol. 1, pp. 80-85, 2010. <br>[16] J. Bora, D. Syiem and S. Bhan, “Quantitative analysis of total phenolic, flavonoid”, J. Pharmacognosy Phytother. vol. 8, pp. 906-911, April 2019. <br>[17] H. Imtara, et al, “Chemical analysis and cytotoxic and cytostatic Effects of twelve honey samples collected from different regions in morocco and Palestine”, Evid Based Complement Alternat Med. vol. 2019, pp. 1-11, May 2019. </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>

CINXE.COM

HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers