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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems.html">Protein Gel Electrophoresis Chamber Systems <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/power-supplies-protein-electrophoresis.html">Electrophoresis Power Supplies <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-staining-imaging.html">Protein Gel and Membrane Stains <span class="nav-list-arrow">&rsaquo;</span></a></li></ul><script> if ( $('#navListActive').children('ul').length > 0 ) { var activeNav = $j('#navListActive'); $j('#navList').prepend(activeNav); } </script></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><p><a id="top"></a>All Invitrogen precast protein gels are high-quality polyacrylamide gels designed to deliver excellent performance, reliability, and reproducibility. Our protein gels are offered in four different chemistries. Whether your protein is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native conditions or by isoelectric point—we have a solution for your application.</p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="brightcoveplayer section"><div data-video="28fc7506f414"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-28fc7506f414', 10); </script></div><div class="video-container video-single"><div id="span-video-28fc7506f414" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-28fc7506f414" class="brightcove-container"><script>document.createElement('video')</script><video id="video-28fc7506f414" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6362478679112" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "WedgeWell precast protein gels", "description": "WedgeWell precast protein gels double the sample loading capacity, making it easier to detect low-abundance or dilute proteins.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/a4e47cc9-4200-4a64-975d-752f08cef95e/8d70c2c7-c2f3-400d-a5f0-8b131661a946/160x90/match/image.jpg"], "uploadDate": "2024-09-26T06:23:08.695Z", "duration": "PT1M45.707S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6362478679112/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6362478679112", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6362478679112&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h3"> On this page: </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><a href="#separation">Four gel chemistries for tailored SDS-PAGE separation</a></li><li><a href="#throughput">High loading capacity meets high sample throughput</a></li><li><a href="#ief">IEF and specialized gels</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul><li><a href="#handcast">Handcast System</a></li><li><a href="#videos">How-to Videos</a></li><li><a href="#related-resources">Related resources</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="heading section"><h2 id="separation"> Four gel chemistries for tailored SDS-PAGE separation </h2></div><div class="text parbase section"><p>Exceptional protein gel electrophoresis is the cornerstone of a successful western blotting experiment. Invitrogen precast protein gels are offered in four different chemistries, allowing you to tailor the separation to your target protein's molecular weight. This helps obtain sharp, well-resolved bands, and results in efficient transfer and improved detection of your protein.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--926122671"><tbody><tr><td width="20%"> </td><td style="text-align: center; padding-left: 0; padding-right: 0;" width="20%"><img src="/content/dam/LifeTech/global/life-sciences/images/bolt-bis-tris-plus-gels-showcase.jpg" alt="Bis-Tris Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels.html">Bis-Tris Gels</a></td><td style="text-align: center; padding-left: 0; padding-right: 0;" width="20%"><img src="/content/dam/LifeTech/global/life-sciences/images/novex-tris-glycine-gels-showcase-22.jpg" alt="Tris-Glycine Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-tris-glycine-gels.html">Tris-Glycine Gels</a></td><td style="text-align: center; padding-left: 0; padding-right: 0;" width="20%"><img src="/content/dam/LifeTech/global/life-sciences/images/nupage-tris-acetate-showcase.jpg" alt="Tris-Acetate Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-tris-acetate-gels.html">Tris-Acetate Gels</a></td><td style="text-align: center; padding-left: 0; padding-right: 0;" width="20%"><img src="/content/dam/LifeTech/global/life-sciences/protein-biology/electrophoresis/tricine-protein-gels-270x195.jpg" alt="Tricine Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/tricine-protein-gels.html">Tricine Gels</a></td></tr><tr><td><b>Available precast gels</b></td><td>NuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus Gels</td><td>Novex Tris-Glycine</td><td>NuPAGE Tris-Acetate</td><td>Novex Tricine</td></tr><tr><td><b>Use for</b></td><td><p>Neutral pH environment helps prevent protein degradation </p><p>Faster run times </p><p>Dilute samples, low abundance proteins</p></td><td>Traditional Laemmli-style gel electrophoresis</td><td>Improved resolution of high molecular weight proteins</td><td>Improved resolution of low molecular weight proteins</td></tr><tr><td><a><b>Protein sample type</b></a></td><td>Broad range MW (6–400 kDa)</td><td>Broad range MW (6–400 kDa)</td><td>High range MW (40–500 kDa)</td><td>Low range MW (2.5–40 kDa)</td></tr><tr><td><b>WedgeWell format gels</b></td><td>Midi and mini (load up to 100 and 60 µL, respectively)</td><td>Midi and mini (load up to 100 and 60 µL, respectively)</td><td>Midi and mini (load up to 100 and 60 µL, respectively)</td><td><p style="text-align: center;">-</p></td></tr><tr><td><b>Average run time</b></td><td>22–45 min Bolt gels 35–50 min NuPAGE</td><td>90 min</td><td>60 min</td><td>90 min</td></tr><tr><td><b>Shelf life*</b></td><td>Up to 16 months at room temperature</td><td>Up to 12 months</td><td>Up to 8 months</td><td>1–2 months</td></tr></tbody></table></div></div><div class="text parbase section"><p>Get started with our money-saving <a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gels-welcome-packs.html">Protein Gel Welcome Packs</a></p><p>Restock with <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-resupply-packs.html" class="cta-link">Protein Gel Resupply Packs</a></p></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="throughput"> High loading capacity meets high sample throughput </h2></div><div class="text parbase section"><p>WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to <a href="#loading">100 µL</a> of sample to be loaded in the well. The WedgeWell design was introduced in 2012 to increase the sample loading capacity of Invitrogen precast mini gels.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_f418"><a class="image-enlarge-link" data-lightbox="image_f418" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/_jcr_content/MainParsys/image_f418/backgroundimg.img.jpg/1731006187663.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.320.low.jpg/1731006187642.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.320.medium.jpg/1731006187642.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.480.medium.jpg/1731006187642.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.620.high.jpg/1731006187642.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.full.high.jpg/1731006187642.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.320.high.jpg/1731006187642.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.480.high.jpg/1731006187642.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.320.medium.jpg/1731006187642.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.620.high.jpg/1731006187642.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.320.low.jpg/1731006187642.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_f418/foregroundimg.img.480.medium.jpg/1731006187642.jpg" alt='Diagram of Wedgewell format well next to conventional well' title='Wedgewell format well vs. conventional well'></picture><style> #cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_f418 img{ width:100%; max-width: 900px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_f418 .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_f418 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt7" comp-id="A2"><div class="text parbase section"><small><b>Figure 1.</b><b>Comparison of a Mini WedgeWell well and traditional Mini gel well shape.</b></small></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p>More than a thousand peer-reviewed articles demonstrate how WedgeWell format gels help produce publication-quality results and enable the study of low abundance proteins, reducing the need to enrich dilute samples before loading them onto the gel.</p></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><p><i>“WedgeWell gels hold up to 60 μL of sample/well making it feasible to load enough protein for dilute OMV suspensions.” <a href="#ref1">[1]</a></i></p><p><i>“The large wedgewells are excellent for loading large volumes.” <a href="#ref2">[2]</a></i></p></div></div></div></div></div></div><div class="text parbase section"><p>Midi gels with WedgeWell format wells allow the separation of up to 26 protein samples in the same run without compromising on sample loading volume.</p><p><b>Key benefits of WedgeWell format gels:</b></p><ul><li><b>Results you can trust</b>—rigorously tested to help ensure reliable, reproducible performance, lot after lot</li><li><b>Easy sample loading</b>—larger well openings make loading a breeze even with standard pipette tips, and reduce sample spill-over and cross well contamination</li><li><b>Increased sample loading capacity</b>—easy detection of dilute samples and low abundance proteins</li><li><b>Reduced cost</b>—lower cost per sample and reduced running buffer required per sample (7.3 mL of running buffer saved per sample, 140 mL saved for 20 samples)*</li><li><b>Faster run times</b>—up to 10 min faster than traditional gels</li></ul><p><b>Available in three different gel chemistries:</b></p><ul><li><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels.html">Bis- Tris</a></li><li><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-tris-glycine-gels.html">Tris-Glycine</a></li><li><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-tris-acetate-gels.html">Tris-Acetate</a></li></ul><p>All Invitrogen protein gels meet the highest standards of performance, reliability, and consistency.</p><p>See our <a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gels-performance-guarantee.html">Protein Gel Performance Guarantee</a></p><p><small>*Based on comparison between our most popular mini and midi well format options (12 and 20 well, respectively)</small></p></div><div class="heading section"><h3 id="loading"><br>Recommended sample loading volumes for mini and midi gels </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--786902786"><tbody><tr><td width="20%" style="font-weight: bold;">Well size</td><td colspan="3" style="text-align: center; font-weight: bold;">Maximum loading volume (µL)</td></tr><tr><td>  </td><th width="30%">WedgeWell format midi gels</th><th width="30%">Standard midi gels</th></tr><tr><td>12 + 2 (small wells) </td><td>100 + 35 </td><td>50 + 15 </td></tr><tr><td>20 </td><td>60 </td><td>30 </td></tr><tr><td>26 </td><td>40 </td><td>20 </td></tr><tr><td>  </td><th>WedgeWell format mini gels</th><th>Standard mini gels</th></tr><tr><td>10 </td><td>60 </td><td>25 </td></tr><tr><td>12 </td><td>45 </td><td>20 </td></tr><tr><td>15 </td><td>35 </td><td>15 </td></tr><tr><td>17 </td><td>30 </td><td>15 </td></tr></tbody></table></div><small>*1 mm thick gel</small></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="ief"> IEF and specialized gels </h2></div><div class="text parbase section"><p>For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/nativepage-bis-tris-gels.html">NativePAGE Bis-Tris</a>, <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-tris-glycine-gels.html">Novex Tris-Glycine</a> and <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-tris-acetate-gels.html">NuPAGE Tris-Acetate</a>. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.</p><p>For separation of proteins by isoelectroctric point (pI), use <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/novex-ief-gels.html">Novex IEF gels</a>. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-175525899"><tbody><tr><td> </td><td style="text-align: center;" width="21%"><img src="/content/dam/LifeTech/global/life-sciences/protein-biology/electrophoresis/CO211094-Bolt-Tris-gel-BG04120BOX(OK-to-use-for-NativePAGE-Bris)270x195.png" alt="NativePAGE Bis-Tris Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/nativepage-bis-tris-gels.html">NativePAGE Bis-Tris Gels</a><br></td><td style="text-align: center;" width="21%"><img src="/content/dam/LifeTech/global/life-sciences/protein-biology/electrophoresis/isoelectric-focusing-gels-270x195.jpg" alt="Isoelectric Focusing Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/novex-ief-gels.html">Isoelectric Focusing Gels</a><br></td><td style="text-align: center;" width="21%"><img src="/content/dam/LifeTech/global/life-sciences/protein-biology/electrophoresis/zymogram-gels-270x195.jpg" alt="Novex Zymogram Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/zymogram-gels.html">Novex Zymogram Gels</a><br></td><td style="text-align: center;" width="21%"><img src="/content/dam/LifeTech/global/life-sciences/images/epage-small.jpg" alt="E-PAGE Precast Gels" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/epage-high-throughput-gel-system.html">E-PAGE Precast<br> Gels</a><br></td></tr><tr><td><b>Gel chemistry</b></td><td>Bis-Tris</td><td>pH 3–7, pH 3–10</td><td>Tris-glycine with gelatin</td><td>8% or 6%</td></tr><tr><td><b>Protein sample type</b></td><td>15–10,000 kDa</td><td>pI 3.5–8.5</td><td>10–220 kDa</td><td>10–200 kDa</td></tr><tr><td><b>Product benefits</b></td><td>Separation of native proteins, neutral pH for high sensitivity applications</td><td>Separation of proteins based on isoelectric point</td><td>Separation of proteins based on size and activity</td><td>High-throughput gel system, that eliminates the need for buffers</td></tr></tbody></table></div></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="handcast"> Handcast System </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-603696372"><tbody><tr><td width="16%"> </td><td style="text-align: center;" width="28%"><img src="/content/dam/LifeTech/global/life-sciences/protein-biology/electrophoresis/surecast-gel-handcast-system-270x195.jpg" alt="SureCast Handcast System" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/surecast-gel-handcast-system.html">SureCast Handcast System</a><br></td><td style="text-align: center;" width="28%"><img src="/content/dam/LifeTech/global/life-sciences/images/novex-empty-casette-small.jpg" alt="Empty Gel Cassettes" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-casting-cassettes.html">Empty Gel Cassettes</a><br></td><td style="text-align: center;" width="28%"><img src="/content/dam/LifeTech/global/life-sciences/images/empty-cassettes-100x70.jpg" alt="Bolt Empty Cassettes" width="130"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-casting-cassettes.html">Bolt Empty Cassettes</a></td></tr><tr><td><b>Cassette type</b></td><td>Reusable, glass</td><td>Single use, pre-sealed</td><td>Single use, pre-sealed</td></tr><tr><td><b>Product benefits</b></td><td><ul><li>Leak-free design—minimize the number of failed gels, waste less time</li><li>Superior glass plate durability</li><li>Unique tilt feature—helps minimize spillage and confidently load acrylamide solutions</li><li>Simple component assembly—using a single-motion load-and-lock mechanism</li></ul></td><td><ul><li>Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)</li><li>Special coating provides uniform polymerization rate for a more consistent handcast gel</li></ul></td><td><ul><li>High capacity sample wells—load up to 60 µL per well</li><li>Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)</li><li>Special coating provides uniform polymerization rate for a more consistent handcast gel</li></ul></td></tr><tr><td><b>Gel dimensions</b></td><td>Mini: 8x8 cm (1 mm thick)</td><td>Mini: 8x8 cm (1 or 1.5 mm thick) Midi: 8x13 cm (1 mm thick)</td><td>Mini: 8x8 cm (1 mm thick)</td></tr><tr><td><b>Gel tank</b></td><td>Mini Gel Tank, Xcell SureLock Mini-Cell</td><td>Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with <a href="/order/catalog/product/WA0999">adapters</a> only)</td><td>Mini Gel Tank, Xcell SureLock Mini-Cell</td></tr><tr><td><b>Reagents</b></td><td>SureCast reagents, other popular polyacrylamide reagents</td><td>SureCast reagents, other popular polyacrylamide reagents</td><td>SureCast reagents, other popular polyacrylamide reagents</td></tr><tr><td><b>Combos available</b></td><td>1-, 2-, 10-, 12-, and 15-wells</td><td>1-, 2-, 5-, 10-, 12-, 15-, and 2D-wells</td><td>10- or 12-wells (high capacity wells)</td></tr></tbody></table></div></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="videos"> How-to videos </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="brightcoveplayer section"><div data-video="f9b5d6f075e5"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-f9b5d6f075e5', 10); </script></div><div class="video-container video-single"><div id="span-video-f9b5d6f075e5" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-f9b5d6f075e5" class="brightcove-container"><script>document.createElement('video')</script><video id="video-f9b5d6f075e5" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="5780093546001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Choosing the right protein gel for your research application", "description": "This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications and much more. ", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/c299fca3-0417-419d-9c2e-92239875e8ff/4960bcb7-55d9-4b5d-b671-5ba501a1f474/160x90/match/image.jpg"], "uploadDate": "2018-05-03T21:15:53.970Z", "duration": "PT44M21S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/5780093546001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5780093546001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5780093546001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="text parbase section"><h3>How to choose the right protein gel for your research application</h3><p>This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications, and how to introduce the right gel chemistry in your research workflow.</p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="brightcoveplayer section"><div data-video="233ddd293327"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-233ddd293327', 10); </script></div><div class="video-container video-single"><div id="span-video-233ddd293327" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-233ddd293327" class="brightcove-container"><script>document.createElement('video')</script><video id="video-233ddd293327" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6059955515001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to separate proteins using an Invitrogen precast SDS-PAGE gel", "description": "Learn how to separate proteins by using a Invitrogen Bolt Bis-Tris Plus SDS-PAGE precast gel and Mini Gel Tank", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/dc1e3135-8650-4350-800f-d1147dad47a6/f9858bbd-5b03-4a32-a8f0-ce483f56c371/160x90/match/image.jpg"], "uploadDate": "2019-07-16T19:58:31.270Z", "duration": "PT5M24.203S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6059955515001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6059955515001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6059955515001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="text parbase section"><h3>How to separate proteins using an Invitrogen precast SDS-PAGE gel</h3><p>This video will show you how to separate proteins by using Invitrogen Mini Gel Tank and Invitrogen precast SDS-PAGE gels.</p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="well-cmp section"><div class="well well-white well-mini"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2" id="related-resources"> Related resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_5af"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-selection-guide.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.full.high.png/1731006187968.png' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.full.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.320.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.480.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.320.medium.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.620.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.320.low.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.480.medium.png/1731006187968.png" alt='Protein Gel Selection Guide' title='Protein Gel Selection Guide'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_5af .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_5af .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="text parbase section"><p><b><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-selection-guide.html">Protein Gel Selection Guide</a></b></p><p>Find an excellent mini or midi gel for your protein electrophoresis experiment.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_e09d"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-conversion-tool.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.full.high.jpg/1731006188006.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.full.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.320.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.480.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.320.medium.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.620.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.320.low.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.480.medium.jpg/1731006188006.jpg" alt='Gel Selection Chart' title='Gel Selection Chart'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_e09d .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_e09d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="text parbase section"><p><b><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-conversion-tool.html">Protein Gel Conversion Tool</a></b></p><p>Find an exceptional Invitrogen mini or midi gel to replace your current precast gel from another supplier.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_2a38"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target='_blank'><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.full.high.jpg/1731006188053.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.full.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.320.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.480.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.320.medium.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.620.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.320.low.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.480.medium.jpg/1731006188053.jpg" alt='Protein Gel Options Brochure' title='Protein Gel Options Brochure'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_2a38 .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_2a38 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="C2"><div class="text parbase section"><p><b><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target="_blank">Gel Migration Chart</a></b></p><p>Download the Protein Gel Guide for a printable gel migration chart.</p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h4>Still unsure which precast gel works best for your research needs? Download the <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/precast-gel-selection-guide.pdf" target="_blank">Gel Selection Chart</a></h4></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_bb7f"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-selection-guide.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.full.high.png/1731006188091.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.full.high.png/1731006188091.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.320.high.png/1731006188091.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.480.high.png/1731006188091.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.320.medium.png/1731006188091.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.620.high.png/1731006188091.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.320.low.png/1731006188091.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/image_bb7f/foregroundimg.img.480.medium.png/1731006188091.png" alt='Protein Gel Selection Guide' title='Protein Gel Selection Guide'></picture><style> #cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_bb7f img{ width:100%; max-width: 200px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_bb7f .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsysimage_bb7f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_7440uipartextimage_797c"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.full.high.png/1731006188138.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.full.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.320.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.480.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.320.medium.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.620.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.320.low.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.480.medium.png/1731006188138.png" alt='Protein Gels Performance Guarantee' title='Protein Gels Performance Guarantee'></picture></div><style> #cq-textimage-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_7440uipartextimage_797c { max-width: 100%; } #cq-textimage-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_7440uipartextimage_797c img { width: 100%; max-width: 263px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3>Protein Gels Performance Guarantee</h3><p>We stand behind the performance of our protein gels.</p><p><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gels-performance-guarantee.html" class="cta-link">Learn more</a></p></div></div><div class="clearfix"></div></div></div></div></div></div></div><div class="heading section"><h2 id="references"> References </h2></div><div class="text parbase section"><ol><li><a id="ref1"></a>Libardo, M.D.J.; Durr, E.; Hernandez, L.D. A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable <i>Haemophilus influenzae</i>.<i>Methods Protoc.</i>2023,<i>6</i>, 42. <a href="https://doi.org/10.3390/mps6020042" target="_blank">https://doi.org/10.3390/mps6020042</a><span class="icon icon-outbound"></span></li><li><a id="ref2"></a>Horita, H., Law, A., Middleton, K. Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins. J. Vis. Exp. (131), e56912, doi:<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908451/" target="_blank">10.3791/56912</a> (2018).<b><i></i></b></li></ol></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt4"><div class="parsys_column cq-colctrl-lt4-c0"><div container-id="cq-colctrl-lt4" comp-id="A1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_4e94"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.full.high.jpg/1706109796911.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.full.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.320.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.480.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.320.medium.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.620.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.320.low.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.480.medium.jpg/1706109796911.jpg" alt='Protein Gel Guide' title='Protein Gel Guide'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_4e94 { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_4e94 img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Download: <a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target="_blank">Protein Gel Guide</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c1"><div container-id="cq-colctrl-lt4" comp-id="B1"><div class="textimage parbase textimage-left section"><div class="image" 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srcset="https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.full.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.320.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.480.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.320.medium.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.620.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.320.low.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.480.medium.jpg/1706109797029.jpg" alt='Protein gel electrophoresis technical handbook' title='Protein gel electrophoresis technical handbook'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_55c0 { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_55c0 img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Download: <a href="/sg/en/home/global/forms/protein-gel-electrophoresis-western-blotting-handbooks.html" target="_blank">Protein gel electrophoresis technical handbook</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c2"><div container-id="cq-colctrl-lt4" comp-id="C1"><div class="textimage parbase textimage-top 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alt='Western Detection Workflow Brochure' title='Western Detection Workflow Brochure'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_183d { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_183d img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Download: <a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/western-workflow-overview-brochure.pdf" target="_blank">Western Detection Workflow brochure</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c3"><div container-id="cq-colctrl-lt4" comp-id="D1"><div class="textimage parbase textimage-top section"><div class="image" 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alt='Protocols, tips &amp; tricks and electrophoresis troubleshooting' title='Protocols, tips &amp; tricks and electrophoresis troubleshooting'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_6ecc { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_6ecc img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Access: <a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-gel-electrophoresis-information.html">Protocols, tips & tricks, and electrophoresis troubleshooting</a></p></div></div><div class="clearfix"></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_d1d9 LTRawHTML"><style> /* Remove horizontal scroll on hero banner table */ .wellPageHeading .well-container .table-component-wrapper table {width:100% !important;} .content.wide-template .container-wrap.template-2-column .span3.sidebar > .container-leftnav {margin-right:20px;} /*.railsection {padding-right:10px;}*/ .textimage {margin-bottom:0px;} /* Label display for author */ .ux-tool-label { display: none; border-left: 50px #dcdcdc solid; padding-left: 10px; font-style: italic; font-size: 14px; } .cq-wcm-edit .ux-tool-label {display: block;} .wellPageHeading h1 b, .wellPageHeading h1 strong {font-weight:normal !important;} .wellPageHeading table td > * {text-shadow: none !important;} </style><div class="ux-tool-label ">Stylesheet for Classic Wide Template adjustments</div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_a06 LTRawHTML"><style> /* Hero Banner Dark Grandient */ .wellPageHeading .well-content { background-image: linear-gradient(-45deg,rgba(68,68,68,0),rgba(0,0,0,.686)); } /* Label display for author */ .ux-tool-label { display: none; border-left: 50px #dcdcdc solid; padding-left: 10px; font-style: italic; font-size: 14px; } .cq-wcm-edit .ux-tool-label {display: block;} </style><div class="ux-tool-label ">Hero banner dark gradient overlay</div></div></div></div><div class="LTRawHTML section"><h3>Find the right protein gel for your experiments</h3><table class="table table-cvc" id="cvc-table-id"><thead><tr><th></th><th>Pour your own gels with a handcast system</th><th>Value gels for screening and qualitative protein separation</th><th>Gels based on traditional Laemmli formula with 60uL sample volume capacity</th><th>Highest resolution, longest shelf-life and western optimized gels</th><th>Highest resolution and long shelf-life </th></tr></thead><tfoot><tr><th></th><td><a href="#handcast" class="btn btn-block">View all</a></td><td><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-value-tris-glycine-gels.html" class="btn btn-block">Order now</a></td><td><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-wedgewell-tris-glycine-gels.html?ICID=cvc-protein-gels-c2b1" class="btn btn-block">Order now</a></td><td><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/bolt-bis-tris-gels.html" class="btn btn-block">Order now</a></td><td><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-protein-gels.html?ICID=cvc-protein-gels-c4b1" class="btn btn-block">Order now</a></td></tr></tfoot><tbody><tr><th> </th><td><img alt="photo of gel cassette" src="/content/dam/LifeTech/Images/integration/gel-casette.gif"><br><a href="#handcast">View all handcast systems</a><br></td><td><img src="/content/dam/LifeTech/global/life-sciences/ProteinExpressionAnalysis/Images/0116/novex-value-cvc.png"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-value-tris-glycine-gels.html">Novex™ Value  Tris-Glycine gels</a></td><td><img alt="photo of Novex gel product" src="/content/dam/LifeTech/global/migrationimages/tg-gels-small.gif"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-wedgewell-tris-glycine-gels.html?icid=cvc-protein-gels-c2t1">Novex™ WedgeWell™ Tris-Glycine gels</a><br></td><td><img alt="photo of Bolt gel product" src="/content/dam/LifeTech/global/migrationimages/Bolt-gels-small.gif"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/bolt-bis-tris-gels.html">Bolt™ Gels</a><br></td><td><img alt="photo of NuPAGE gel product" src="/content/dam/LifeTech/global/migrationimages/nupage-bis-tris-small.gif"><br><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-protein-gels.html?icid=cvc-protein-gels-c4t1">NuPAGE™ Gels</a><br><br></td></tr><tr><th><span class="side">Citations in the last 10 years</span></th><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A (new product)</td><td>>20,000</td></tr><tr><th><span class="side">Protein resolution & integrity, lot consistency & band quality</span></th><td>Not applicable</td><td>Good</td><td>High</td><td>High</td><td><span class="on">High</span></td></tr><tr><th><span class="side">Average run time</span></th><td>90 min</td><td>46 min (225V)</td><td>60 min</td><td>35 min</td><td><span class="on">35 min</span></td></tr><tr><th><span class="side">Well format</span></th><td>Standard</td><td>Standard</td><td>New high-capacity wedge design</td><td><b>New high-capacity wedge design</b></td><td><span class="on">Standard</span></td></tr><tr><th><span class="side">Gel dimensions</span></th><td>Mini: 8x8 cm, 1 or 1.5 mm thick</td><td>Mini: 8x8 cm, 1 mm thick</td><td>Mini: 8x8 cm, 1 or 1.5 mm thick </td><td>Mini: 8x8.3 cm, 1 mm thick</td><td><span class="on">Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick</span></td></tr><tr><th><span class="side">Shelf life</span></th><td>N/A</td><td>Up to 12 months</td><td>Up to 12 months</td><td>>16 months</td><td><span class="on">8 months (Tris-Acetate) 16 months (Bis-Tris)</span></td></tr><tr><th><span class="side">Pack sizes</span></th><td>N/A</td><td>20 pack</td><td>2 & 10 packs</td><td>10 packs</td><td><span class="on">2 & 10 packs</span></td></tr></tbody></table><a name="handcast"></a><script> $j('#cvc-table-id').cvc({ active: 1 }); </script></div></div><div><div class="MainInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="MainInheritParsys iparsys parsys"><div 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